AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22938

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22938.t1 AT3G42170 30.081 615 364 10 16 588 38 628 3.29e-92 298
MS.gene22938.t1 AT3G42170 30.081 615 364 10 16 588 60 650 5.09e-92 298
MS.gene22938.t1 AT1G18560 22.690 617 417 16 4 587 35 624 3.69e-29 123

Find 115 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTGATTTGCAAAGTTATT+TGG 0.184869 1.2:-58685226 MS.gene22938:CDS
ACCTCATACCGGAGTTAATT+TGG 0.197408 1.2:-58686219 MS.gene22938:CDS
TCAAGCGACTTCGCAGATTT+TGG 0.228777 1.2:-58685288 MS.gene22938:CDS
CTAAGTCTTCATATTTCTTA+TGG 0.231556 1.2:+58685187 None:intergenic
AAATTTAACCTTCATTCTTT+TGG 0.248075 1.2:+58685548 None:intergenic
AATATAGCCCATCAACTATA+AGG 0.258780 1.2:+58686066 None:intergenic
GAGAAGTATCTCGATGAAAA+TGG 0.274133 1.2:-58686713 MS.gene22938:CDS
TATTTCTTATGGTCAAGTTT+TGG 0.294624 1.2:+58685198 None:intergenic
TCCTCACTTTGATAATTTAT+CGG 0.298218 1.2:-58685769 MS.gene22938:CDS
GATGTTAAACAAATCTTTCC+TGG 0.303845 1.2:+58686353 None:intergenic
TTGATAGTGCTATCGGTCTA+AGG 0.315126 1.2:-58685821 MS.gene22938:CDS
ACTTAGATATCCTTGAATAT+TGG 0.317299 1.2:-58685170 MS.gene22938:CDS
TCTGATGCAACAGTGGTAAT+TGG 0.317328 1.2:+58685087 None:intergenic
AAAACTTGAAATGTTATTTA+CGG 0.321785 1.2:-58686382 MS.gene22938:CDS
AGGTTAAATTTGATAAATAC+TGG 0.324743 1.2:-58685536 MS.gene22938:CDS
GGACCTTCCCTTATAGTTGA+TGG 0.328939 1.2:-58686074 MS.gene22938:CDS
TCTTCACAAAGCTCTGTTAG+AGG 0.329113 1.2:-58685327 MS.gene22938:CDS
AATCGTGCATGAAGGTATTA+AGG 0.329425 1.2:-58686009 MS.gene22938:CDS
TTTCTTACACCCTCATGTTA+AGG 0.346975 1.2:-58686462 MS.gene22938:CDS
TACTAAGTCTGTTGAGAGAT+TGG 0.358442 1.2:-58686187 MS.gene22938:CDS
TTGAAGAGACACCCATCATT+TGG 0.365868 1.2:-58686095 MS.gene22938:CDS
CATTTGCTTTCCAATATTCA+AGG 0.377489 1.2:+58685160 None:intergenic
GATAAATACAGATCAATATA+TGG 0.380480 1.2:-58686315 MS.gene22938:CDS
ACTAAGTCTGTTGAGAGATT+GGG 0.382513 1.2:-58686186 MS.gene22938:CDS
TTCTTACACCCTCATGTTAA+GGG 0.383140 1.2:-58686461 MS.gene22938:CDS
ACTATCAAGCATCAAATAAC+TGG 0.386923 1.2:+58685836 None:intergenic
AGAATATCTCAAGTCCTCTA+AGG 0.396230 1.2:-58685364 MS.gene22938:CDS
TGAACCACTTATCTCCTTAG+AGG 0.397384 1.2:+58685350 None:intergenic
TGTTATTTACGGTCAATTCC+AGG 0.400629 1.2:-58686371 MS.gene22938:CDS
TCCGTCAAGACATGCCTATT+CGG 0.404416 1.2:-58685866 MS.gene22938:CDS
ACAAATTGTAACTTAAATCT+AGG 0.407030 1.2:+58685471 None:intergenic
CAACAGACTTAGTAACTTCT+CGG 0.418812 1.2:+58686196 None:intergenic
GATATCACAACTTTGTTCTC+GGG 0.422383 1.2:-58685693 MS.gene22938:CDS
GACCTTCCCTTATAGTTGAT+GGG 0.422938 1.2:-58686073 MS.gene22938:CDS
CAATTTGTTGAGTATTGCTA+TGG 0.423448 1.2:-58685456 MS.gene22938:CDS
TGATATCACAACTTTGTTCT+CGG 0.425432 1.2:-58685694 MS.gene22938:CDS
TAAGGCAGTAGGTGCTTCCT+TGG 0.429066 1.2:-58685991 MS.gene22938:CDS
TTGATCTAGTGGTGGATAAG+AGG 0.429575 1.2:+58686577 None:intergenic
TATTGCTATGGGAAGTTGTT+TGG 0.432386 1.2:-58685444 MS.gene22938:CDS
CGTGCCATCATAGCTACTTC+AGG 0.434074 1.2:+58685126 None:intergenic
CAATTTAAGCATCTTGCCCT+TGG 0.450065 1.2:+58686686 None:intergenic
TGAAAGAATAACACTGTAAG+AGG 0.455420 1.2:+58685512 None:intergenic
TGTATAGGAAATATGTCAAA+TGG 0.455993 1.2:-58686815 None:intergenic
ACCGAATAGGCATGTCTTGA+CGG 0.464850 1.2:+58685865 None:intergenic
TAACTGGAATTCCACCGAAT+AGG 0.476280 1.2:+58685852 None:intergenic
ATATAGCCCATCAACTATAA+GGG 0.478825 1.2:+58686067 None:intergenic
GGGAAGTTGTTTGGCAGCGA+TGG 0.482165 1.2:-58685435 MS.gene22938:CDS
GCCAAATTAACTCCGGTATG+AGG 0.484940 1.2:+58686218 None:intergenic
TCATGTTCAACAAATGCAAA+AGG 0.491745 1.2:+58686506 None:intergenic
TGTTGAACATGACGGAATTG+AGG 0.491872 1.2:-58686495 MS.gene22938:CDS
AAGTCGCTTGATGTAATTTG+TGG 0.492040 1.2:+58685300 None:intergenic
CTTTGATAATTTATCGGACG+AGG 0.492397 1.2:-58685763 MS.gene22938:CDS
ATAAGGGAAGGTCCAAATGA+TGG 0.495352 1.2:+58686083 None:intergenic
CACTTTCCACCACCTCATAC+CGG 0.496648 1.2:-58686230 MS.gene22938:CDS
TTGATGCTTGATAGTGCTAT+CGG 0.497524 1.2:-58685828 MS.gene22938:CDS
CACTAGATCAAGAAAAGTAC+AGG 0.497708 1.2:-58686565 MS.gene22938:CDS
GTGTGTGTGAAGTATATAAG+AGG 0.503464 1.2:-58685957 MS.gene22938:CDS
AAAGCTGTATAGAAAAGAAA+AGG 0.503976 1.2:-58686405 MS.gene22938:CDS
TCCACACACCACTAAAATAG+AGG 0.508049 1.2:+58685649 None:intergenic
TCAGAGTCATCGTTCAGTAT+AGG 0.511150 1.2:-58685069 MS.gene22938:CDS
ATATCCTGAAGTAGCTATGA+TGG 0.513630 1.2:-58685130 MS.gene22938:CDS
GAGGTTGGCGGTTGGATAAG+TGG 0.515344 1.2:+58685668 None:intergenic
AGTCGCTTGATGTAATTTGT+GGG 0.523372 1.2:+58685301 None:intergenic
ATCGGACGAGGAGTGGATTG+AGG 0.527724 1.2:-58685751 MS.gene22938:CDS
GGAAGCATTTACCCTCCTAC+AGG 0.531501 1.2:-58685799 MS.gene22938:CDS
TTGAATTTAATCGTGCATGA+AGG 0.532005 1.2:-58686017 MS.gene22938:CDS
AAAGTTGTGATATCATAAAA+CGG 0.532565 1.2:+58685702 None:intergenic
TGTTGAAATGTGTCGAAGGA+GGG 0.534694 1.2:-58686607 MS.gene22938:CDS
GAATATTGGAAAGCAAATGA+AGG 0.535758 1.2:-58685156 MS.gene22938:CDS
TTAACTCCGGTATGAGGTGG+TGG 0.539866 1.2:+58686224 None:intergenic
GAGAATTCAGATGAAAATCA+AGG 0.542293 1.2:-58685628 MS.gene22938:CDS
ATAATTTATCGGACGAGGAG+TGG 0.544212 1.2:-58685758 MS.gene22938:CDS
CTAAGTCTGTTGAGAGATTG+GGG 0.547500 1.2:-58686185 MS.gene22938:CDS
CTAAAATAGAGGTTGGCGGT+TGG 0.556005 1.2:+58685660 None:intergenic
TTGTTGAAATGTGTCGAAGG+AGG 0.556585 1.2:-58686608 MS.gene22938:CDS
GAGGATCAATATCCTGTAGG+AGG 0.560275 1.2:+58685787 None:intergenic
ATAGGAAATATGTCAAATGG+AGG 0.561206 1.2:-58686812 None:intergenic
GTACAGGGAGAAGATAGCTC+AGG 0.562417 1.2:-58686549 MS.gene22938:CDS
TAAGGGAAGGTCCAAATGAT+GGG 0.562550 1.2:+58686084 None:intergenic
TAGGAAATATGTCAAATGGA+GGG 0.563980 1.2:-58686811 None:intergenic
TTGCATGGCCAAAAGAATGA+AGG 0.567331 1.2:-58685556 MS.gene22938:CDS
TCTTACACCCTCATGTTAAG+GGG 0.574472 1.2:-58686460 MS.gene22938:CDS
CTAGAAATCCCCTTAACATG+AGG 0.576333 1.2:+58686452 None:intergenic
TGATAGTGCTATCGGTCTAA+GGG 0.578435 1.2:-58685820 MS.gene22938:CDS
AATATTGGAAAGCAAATGAA+GGG 0.581923 1.2:-58685155 MS.gene22938:CDS
CATGAAGGTATTAAGGCAGT+AGG 0.586618 1.2:-58686002 MS.gene22938:CDS
GTTGAAATGTGTCGAAGGAG+GGG 0.586766 1.2:-58686606 MS.gene22938:CDS
CACACCACTAAAATAGAGGT+TGG 0.588765 1.2:+58685653 None:intergenic
CGATGAAAATGGACAACCAA+GGG 0.591453 1.2:-58686702 MS.gene22938:CDS
TTTGCATTTGTTGAACATGA+CGG 0.591766 1.2:-58686503 MS.gene22938:CDS
TAGAAATCCCCTTAACATGA+GGG 0.592320 1.2:+58686453 None:intergenic
AAGTCCTCTAAGGAGATAAG+TGG 0.598076 1.2:-58685354 MS.gene22938:CDS
GAGTCATCGTTCAGTATAGG+TGG 0.599118 1.2:-58685066 MS.gene22938:CDS
TGCATCTAACAATGATGCAA+TGG 0.600721 1.2:-58686126 MS.gene22938:CDS
TCCGATAAATTATCAAAGTG+AGG 0.601110 1.2:+58685768 None:intergenic
AGGAAATATGTCAAATGGAG+GGG 0.603196 1.2:-58686810 None:intergenic
AGCCCATCAACTATAAGGGA+AGG 0.603377 1.2:+58686071 None:intergenic
TCAAGATCAAGTATGTTGCA+TGG 0.606681 1.2:-58685571 MS.gene22938:CDS
TTTGTTTAACATCTGACTTG+TGG 0.607895 1.2:-58686343 MS.gene22938:CDS
AGTGAGGATCAATATCCTGT+AGG 0.613873 1.2:+58685784 None:intergenic
AGATGCATTATCTAGAGTAA+TGG 0.615581 1.2:+58686142 None:intergenic
ACCACTAAAATAGAGGTTGG+CGG 0.616373 1.2:+58685656 None:intergenic
GTCAAGACATGCCTATTCGG+TGG 0.621491 1.2:-58685863 MS.gene22938:CDS
AGGTGGTGGAAAGTGAAAGA+AGG 0.624244 1.2:+58686238 None:intergenic
ACACACTCTGATCTTATCCA+AGG 0.627975 1.2:+58685974 None:intergenic
CATTTGTTGAAATGTGTCGA+AGG 0.632206 1.2:-58686611 MS.gene22938:CDS
AATTTGTTGAGTATTGCTAT+GGG 0.633359 1.2:-58685455 MS.gene22938:CDS
AAATTAACTCCGGTATGAGG+TGG 0.635735 1.2:+58686221 None:intergenic
AGGATCAATATCCTGTAGGA+GGG 0.640224 1.2:+58685788 None:intergenic
CTTCTCGGCCAAATTAACTC+CGG 0.643512 1.2:+58686211 None:intergenic
GAAGTATATAAGAGGCTCAG+AGG 0.671452 1.2:-58685949 MS.gene22938:CDS
CGATGACTCTGATGCAACAG+TGG 0.679734 1.2:+58685080 None:intergenic
AAATTTCAAAGTAAACTGTG+TGG 0.702647 1.2:-58685264 MS.gene22938:CDS
ACTAGATCAAGAAAAGTACA+GGG 0.709367 1.2:-58686564 MS.gene22938:CDS
TCGATGAAAATGGACAACCA+AGG 0.709707 1.2:-58686703 MS.gene22938:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAACTTGAAATGTTATTTA+CGG - chr1.2:58685468-58685487 MS.gene22938:CDS 15.0%
!! AAAGTTGTGATATCATAAAA+CGG + chr1.2:58686151-58686170 None:intergenic 20.0%
!! ACAAATTGTAACTTAAATCT+AGG + chr1.2:58686382-58686401 None:intergenic 20.0%
!! AGGTTAAATTTGATAAATAC+TGG - chr1.2:58686314-58686333 MS.gene22938:CDS 20.0%
!! GATAAATACAGATCAATATA+TGG - chr1.2:58685535-58685554 MS.gene22938:CDS 20.0%
!!! AAATTTAACCTTCATTCTTT+TGG + chr1.2:58686305-58686324 None:intergenic 20.0%
! AAAGCTGTATAGAAAAGAAA+AGG - chr1.2:58685445-58685464 MS.gene22938:CDS 25.0%
! AAATTTCAAAGTAAACTGTG+TGG - chr1.2:58686586-58686605 MS.gene22938:CDS 25.0%
! AATATTGGAAAGCAAATGAA+GGG - chr1.2:58686695-58686714 MS.gene22938:CDS 25.0%
! ACTTAGATATCCTTGAATAT+TGG - chr1.2:58686680-58686699 MS.gene22938:CDS 25.0%
! CTAAGTCTTCATATTTCTTA+TGG + chr1.2:58686666-58686685 None:intergenic 25.0%
!! TATATTTTCATGTACGATGT+GGG - chr1.2:58685801-58685820 MS.gene22938:CDS 25.0%
!! TATTTCTTATGGTCAAGTTT+TGG + chr1.2:58686655-58686674 None:intergenic 25.0%
!! TCCTCACTTTGATAATTTAT+CGG - chr1.2:58686081-58686100 MS.gene22938:CDS 25.0%
!!! AATTTGTTGAGTATTGCTAT+GGG - chr1.2:58686395-58686414 MS.gene22938:CDS 25.0%
!!! GTTTTTGGTTTTTTTGAACT+TGG + chr1.2:58685095-58685114 None:intergenic 25.0%
AAAAAGCTTTCAGCTTATCA+AGG + chr1.2:58686462-58686481 None:intergenic 30.0%
AACCAAAAACTTCAGTAGTT+TGG - chr1.2:58685105-58685124 MS.gene22938:CDS 30.0%
AATATAGCCCATCAACTATA+AGG + chr1.2:58685787-58685806 None:intergenic 30.0%
ACCAAAAACTTCAGTAGTTT+GGG - chr1.2:58685106-58685125 MS.gene22938:CDS 30.0%
ACTATCAAGCATCAAATAAC+TGG + chr1.2:58686017-58686036 None:intergenic 30.0%
ATATAGCCCATCAACTATAA+GGG + chr1.2:58685786-58685805 None:intergenic 30.0%
CATTTGCTTTCCAATATTCA+AGG + chr1.2:58686693-58686712 None:intergenic 30.0%
GAATATTGGAAAGCAAATGA+AGG - chr1.2:58686694-58686713 MS.gene22938:CDS 30.0%
GAGAATTCAGATGAAAATCA+AGG - chr1.2:58686222-58686241 MS.gene22938:CDS 30.0%
GATGTTAAACAAATCTTTCC+TGG + chr1.2:58685500-58685519 None:intergenic 30.0%
TCATGTTCAACAAATGCAAA+AGG + chr1.2:58685347-58685366 None:intergenic 30.0%
TCCGATAAATTATCAAAGTG+AGG + chr1.2:58686085-58686104 None:intergenic 30.0%
TGAAAGAATAACACTGTAAG+AGG + chr1.2:58686341-58686360 None:intergenic 30.0%
TGATATCACAACTTTGTTCT+CGG - chr1.2:58686156-58686175 MS.gene22938:CDS 30.0%
TTGAATTTAATCGTGCATGA+AGG - chr1.2:58685833-58685852 MS.gene22938:CDS 30.0%
! AAATCAGACTCTTTTCTTGA+CGG + chr1.2:58686613-58686632 None:intergenic 30.0%
! ACTAGATCAAGAAAAGTACA+GGG - chr1.2:58685286-58685305 MS.gene22938:CDS 30.0%
! AGATGCATTATCTAGAGTAA+TGG + chr1.2:58685711-58685730 None:intergenic 30.0%
! ATATTTTCATGTACGATGTG+GGG - chr1.2:58685802-58685821 MS.gene22938:CDS 30.0%
! CTATATTTTCATGTACGATG+TGG - chr1.2:58685800-58685819 MS.gene22938:CDS 30.0%
! GTCTGATTTGCAAAGTTATT+TGG - chr1.2:58686624-58686643 MS.gene22938:CDS 30.0%
! TTTGCATTTGTTGAACATGA+CGG - chr1.2:58685347-58685366 MS.gene22938:CDS 30.0%
! TTTGTTTAACATCTGACTTG+TGG - chr1.2:58685507-58685526 MS.gene22938:CDS 30.0%
!! CAATTTGTTGAGTATTGCTA+TGG - chr1.2:58686394-58686413 MS.gene22938:CDS 30.0%
AAGTCGCTTGATGTAATTTG+TGG + chr1.2:58686553-58686572 None:intergenic 35.0%
ACTAAGTCTGTTGAGAGATT+GGG - chr1.2:58685664-58685683 MS.gene22938:CDS 35.0%
AGAATATCTCAAGTCCTCTA+AGG - chr1.2:58686486-58686505 MS.gene22938:CDS 35.0%
ATATCCTGAAGTAGCTATGA+TGG - chr1.2:58686720-58686739 MS.gene22938:CDS 35.0%
CAACAGACTTAGTAACTTCT+CGG + chr1.2:58685657-58685676 None:intergenic 35.0%
GATATCACAACTTTGTTCTC+GGG - chr1.2:58686157-58686176 MS.gene22938:CDS 35.0%
GTGTGTGTGAAGTATATAAG+AGG - chr1.2:58685893-58685912 MS.gene22938:CDS 35.0%
TACGCTGCAAAAAATACTAG+TGG - chr1.2:58685197-58685216 MS.gene22938:CDS 35.0%
TACTAAGTCTGTTGAGAGAT+TGG - chr1.2:58685663-58685682 MS.gene22938:CDS 35.0%
TAGAAATCCCCTTAACATGA+GGG + chr1.2:58685400-58685419 None:intergenic 35.0%
TATTGCTATGGGAAGTTGTT+TGG - chr1.2:58686406-58686425 MS.gene22938:CDS 35.0%
TCAAGATCAAGTATGTTGCA+TGG - chr1.2:58686279-58686298 MS.gene22938:CDS 35.0%
TGCATCTAACAATGATGCAA+TGG - chr1.2:58685724-58685743 MS.gene22938:CDS 35.0%
TTCTTACACCCTCATGTTAA+GGG - chr1.2:58685389-58685408 MS.gene22938:CDS 35.0%
TTTCTTACACCCTCATGTTA+AGG - chr1.2:58685388-58685407 MS.gene22938:CDS 35.0%
! ACATTTTGTGAATAGGAGGT+GGG - chr1.2:58685568-58685587 MS.gene22938:CDS 35.0%
! AGTCGCTTGATGTAATTTGT+GGG + chr1.2:58686552-58686571 None:intergenic 35.0%
! CACTAGATCAAGAAAAGTAC+AGG - chr1.2:58685285-58685304 MS.gene22938:CDS 35.0%
! CATTTGTTGAAATGTGTCGA+AGG - chr1.2:58685239-58685258 MS.gene22938:CDS 35.0%
! CTGTACTTTTCTTGATCTAG+TGG + chr1.2:58685287-58685306 None:intergenic 35.0%
! CTTTGATAATTTATCGGACG+AGG - chr1.2:58686087-58686106 MS.gene22938:CDS 35.0%
! TACTTTTCTTGATCTAGTGG+TGG + chr1.2:58685284-58685303 None:intergenic 35.0%
! TCCCAAACTACTGAAGTTTT+TGG + chr1.2:58685110-58685129 None:intergenic 35.0%
! TGACTGCACATTTTGTGAAT+AGG - chr1.2:58685561-58685580 MS.gene22938:CDS 35.0%
! TGTTATTTACGGTCAATTCC+AGG - chr1.2:58685479-58685498 MS.gene22938:CDS 35.0%
!! AATCGTGCATGAAGGTATTA+AGG - chr1.2:58685841-58685860 MS.gene22938:CDS 35.0%
!! GAGAAGTATCTCGATGAAAA+TGG - chr1.2:58685137-58685156 MS.gene22938:CDS 35.0%
!! TTGATGCTTGATAGTGCTAT+CGG - chr1.2:58686022-58686041 MS.gene22938:CDS 35.0%
!!! ACTAGTATTTTTTGCAGCGT+AGG + chr1.2:58685198-58685217 None:intergenic 35.0%
AAATTAACTCCGGTATGAGG+TGG + chr1.2:58685632-58685651 None:intergenic 40.0%
ACACACTCTGATCTTATCCA+AGG + chr1.2:58685879-58685898 None:intergenic 40.0%
ACCACTAAAATAGAGGTTGG+CGG + chr1.2:58686197-58686216 None:intergenic 40.0%
ACCTCATACCGGAGTTAATT+TGG - chr1.2:58685631-58685650 MS.gene22938:CDS 40.0%
AGGATCAATATCCTGTAGGA+GGG + chr1.2:58686065-58686084 None:intergenic 40.0%
AGTGAGGATCAATATCCTGT+AGG + chr1.2:58686069-58686088 None:intergenic 40.0%
ATAAGGGAAGGTCCAAATGA+TGG + chr1.2:58685770-58685789 None:intergenic 40.0%
ATAATTTATCGGACGAGGAG+TGG - chr1.2:58686092-58686111 MS.gene22938:CDS 40.0%
CAATTTAAGCATCTTGCCCT+TGG + chr1.2:58685167-58685186 None:intergenic 40.0%
CACACCACTAAAATAGAGGT+TGG + chr1.2:58686200-58686219 None:intergenic 40.0%
CGATGAAAATGGACAACCAA+GGG - chr1.2:58685148-58685167 MS.gene22938:CDS 40.0%
CTAAGTCTGTTGAGAGATTG+GGG - chr1.2:58685665-58685684 MS.gene22938:CDS 40.0%
CTAGAAATCCCCTTAACATG+AGG + chr1.2:58685401-58685420 None:intergenic 40.0%
GAAGTATATAAGAGGCTCAG+AGG - chr1.2:58685901-58685920 MS.gene22938:CDS 40.0%
GACCTTCCCTTATAGTTGAT+GGG - chr1.2:58685777-58685796 MS.gene22938:CDS 40.0%
TAACTGGAATTCCACCGAAT+AGG + chr1.2:58686001-58686020 None:intergenic 40.0%
TAAGGGAAGGTCCAAATGAT+GGG + chr1.2:58685769-58685788 None:intergenic 40.0%
TCAGAGTCATCGTTCAGTAT+AGG - chr1.2:58686781-58686800 MS.gene22938:CDS 40.0%
TCCACACACCACTAAAATAG+AGG + chr1.2:58686204-58686223 None:intergenic 40.0%
TCGATGAAAATGGACAACCA+AGG - chr1.2:58685147-58685166 MS.gene22938:CDS 40.0%
TCTGATGCAACAGTGGTAAT+TGG + chr1.2:58686766-58686785 None:intergenic 40.0%
TCTTACACCCTCATGTTAAG+GGG - chr1.2:58685390-58685409 MS.gene22938:CDS 40.0%
TCTTCACAAAGCTCTGTTAG+AGG - chr1.2:58686523-58686542 MS.gene22938:CDS 40.0%
TGAACCACTTATCTCCTTAG+AGG + chr1.2:58686503-58686522 None:intergenic 40.0%
TGTTGAAATGTGTCGAAGGA+GGG - chr1.2:58685243-58685262 MS.gene22938:CDS 40.0%
TTGAAGAGACACCCATCATT+TGG - chr1.2:58685755-58685774 MS.gene22938:CDS 40.0%
TTGCATGGCCAAAAGAATGA+AGG - chr1.2:58686294-58686313 MS.gene22938:CDS 40.0%
TTGTTGAAATGTGTCGAAGG+AGG - chr1.2:58685242-58685261 MS.gene22938:CDS 40.0%
! AAGTCCTCTAAGGAGATAAG+TGG - chr1.2:58686496-58686515 MS.gene22938:CDS 40.0%
! CACATTTTGTGAATAGGAGG+TGG - chr1.2:58685567-58685586 MS.gene22938:CDS 40.0%
! CTGCACATTTTGTGAATAGG+AGG - chr1.2:58685564-58685583 MS.gene22938:CDS 40.0%
! GGAGTTAGAGAAAAAAGTGC+TGG - chr1.2:58685589-58685608 MS.gene22938:CDS 40.0%
! TGATAGTGCTATCGGTCTAA+GGG - chr1.2:58686030-58686049 MS.gene22938:CDS 40.0%
! TGTTGAACATGACGGAATTG+AGG - chr1.2:58685355-58685374 MS.gene22938:CDS 40.0%
!! CATGAAGGTATTAAGGCAGT+AGG - chr1.2:58685848-58685867 MS.gene22938:CDS 40.0%
!! TTGATAGTGCTATCGGTCTA+AGG - chr1.2:58686029-58686048 MS.gene22938:CDS 40.0%
!! TTGATCTAGTGGTGGATAAG+AGG + chr1.2:58685276-58685295 None:intergenic 40.0%
!!! ACCTCTATTTTAGTGGTGTG+TGG - chr1.2:58686200-58686219 MS.gene22938:CDS 40.0%
ACCGAATAGGCATGTCTTGA+CGG + chr1.2:58685988-58686007 None:intergenic 45.0%
AGCCCATCAACTATAAGGGA+AGG + chr1.2:58685782-58685801 None:intergenic 45.0%
CTAAAATAGAGGTTGGCGGT+TGG + chr1.2:58686193-58686212 None:intergenic 45.0%
CTTCTCGGCCAAATTAACTC+CGG + chr1.2:58685642-58685661 None:intergenic 45.0%
GAGGATCAATATCCTGTAGG+AGG + chr1.2:58686066-58686085 None:intergenic 45.0%
GAGTCATCGTTCAGTATAGG+TGG - chr1.2:58686784-58686803 MS.gene22938:CDS 45.0%
GCCAAATTAACTCCGGTATG+AGG + chr1.2:58685635-58685654 None:intergenic 45.0%
GGACCTTCCCTTATAGTTGA+TGG - chr1.2:58685776-58685795 MS.gene22938:CDS 45.0%
GTTGAAATGTGTCGAAGGAG+GGG - chr1.2:58685244-58685263 MS.gene22938:CDS 45.0%
TCCGTCAAGACATGCCTATT+CGG - chr1.2:58685984-58686003 MS.gene22938:CDS 45.0%
! TCAAGCGACTTCGCAGATTT+TGG - chr1.2:58686562-58686581 MS.gene22938:CDS 45.0%
!! ACCGCCAACCTCTATTTTAG+TGG - chr1.2:58686193-58686212 MS.gene22938:CDS 45.0%
!! AGGTGGTGGAAAGTGAAAGA+AGG + chr1.2:58685615-58685634 None:intergenic 45.0%
CACTTTCCACCACCTCATAC+CGG - chr1.2:58685620-58685639 MS.gene22938:CDS 50.0%
CGATGACTCTGATGCAACAG+TGG + chr1.2:58686773-58686792 None:intergenic 50.0%
CGTGCCATCATAGCTACTTC+AGG + chr1.2:58686727-58686746 None:intergenic 50.0%
GTACAGGGAGAAGATAGCTC+AGG - chr1.2:58685301-58685320 MS.gene22938:CDS 50.0%
GTCAAGACATGCCTATTCGG+TGG - chr1.2:58685987-58686006 MS.gene22938:CDS 50.0%
TAAGGCAGTAGGTGCTTCCT+TGG - chr1.2:58685859-58685878 MS.gene22938:CDS 50.0%
TTAACTCCGGTATGAGGTGG+TGG + chr1.2:58685629-58685648 None:intergenic 50.0%
!! GGAAGCATTTACCCTCCTAC+AGG - chr1.2:58686051-58686070 MS.gene22938:CDS 50.0%
GAGGTTGGCGGTTGGATAAG+TGG + chr1.2:58686185-58686204 None:intergenic 55.0%
GGGAAGTTGTTTGGCAGCGA+TGG - chr1.2:58686415-58686434 MS.gene22938:CDS 55.0%
!! ATCGGACGAGGAGTGGATTG+AGG - chr1.2:58686099-58686118 MS.gene22938:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 58685047 58686825 58685047 ID=MS.gene22938
chr1.2 mRNA 58685047 58686825 58685047 ID=MS.gene22938.t1;Parent=MS.gene22938
chr1.2 exon 58685047 58686825 58685047 ID=MS.gene22938.t1.exon1;Parent=MS.gene22938.t1
chr1.2 CDS 58685047 58686825 58685047 ID=cds.MS.gene22938.t1;Parent=MS.gene22938.t1
Gene Sequence

>MS.gene22938

ATGTCAAATGGAGGGGATGTCTCTGATGAAGTTGAAAGTTCAGTGCCAAGTTCAAAAAAACCAAAAACTTCAGTAGTTTGGGAGTTTTTTGAGAAGTATCTCGATGAAAATGGACAACCAAGGGCAAGATGCTTAAATTGTGCTACAACCTACGCTGCAAAAAATACTAGTGGTACGTCAAATTTGAAGTCACATTTGTTGAAATGTGTCGAAGGAGGGGATGTTTCCTCTTATCCACCACTAGATCAAGAAAAGTACAGGGAGAAGATAGCTCAGGTGATTGTCAAGAATAGTTATCCTTTTGCATTTGTTGAACATGACGGAATTGAGGATTTGCACTGTTTCTTACACCCTCATGTTAAGGGGATTTCTAGAAACACAGCAAAGTCAGATGTTCTAAAGCTGTATAGAAAAGAAAAGGAAAACTTGAAATGTTATTTACGGTCAATTCCAGGAAAGATTTGTTTAACATCTGACTTGTGGTCATCGATAAATACAGATCAATATATGGTGTTGACTGCACATTTTGTGAATAGGAGGTGGGAGTTAGAGAAAAAAGTGCTGGCCTTCTTTCACTTTCCACCACCTCATACCGGAGTTAATTTGGCCGAGAAGTTACTAAGTCTGTTGAGAGATTGGGGTATCGAGAAAAAGATATTCACCATTACTCTAGATAATGCATCTAACAATGATGCAATGGTGAATATTTTGAAGAGACACCCATCATTTGGACCTTCCCTTATAGTTGATGGGCTATATTTTCATGTACGATGTGGGGCACATGTTTTGAATTTAATCGTGCATGAAGGTATTAAGGCAGTAGGTGCTTCCTTGGATAAGATCAGAGTGTGTGTGAAGTATATAAGAGGCTCAGAGGCACGAAAGATGAAGTTCGCATCATGTTTAGAGCAACTCACACAAGTGACTTCTAAGCAACTCCGTCAAGACATGCCTATTCGGTGGAATTCCAGTTATTTGATGCTTGATAGTGCTATCGGTCTAAGGGAAGCATTTACCCTCCTACAGGATATTGATCCTCACTTTGATAATTTATCGGACGAGGAGTGGATTGAGGCACAAAAAATTGCTGAGTTTTTGAAGCCGTTTTATGATATCACAACTTTGTTCTCGGGTACCACTTATCCAACCGCCAACCTCTATTTTAGTGGTGTGTGGAGAATTCAGATGAAAATCAAGGAAGTTGCATGTGTTTTTCATAGTGAGTTTGAAAATCAAGATCAAGTATGTTGCATGGCCAAAAGAATGAAGGTTAAATTTGATAAATACTGGACCTCTTACAGTGTTATTCTTTCATTTGCTGTCATCTTAGATCCTAGATTTAAGTTACAATTTGTTGAGTATTGCTATGGGAAGTTGTTTGGCAGCGATGGAGAGAAACTTGCAAATGATATCCTTGATAAGCTGAAAGCTTTTTTTCAAGAATATCTCAAGTCCTCTAAGGAGATAAGTGGTTCATCTTCACAAAGCTCTGTTAGAGGTAGCCCACAAATTACATCAAGCGACTTCGCAGATTTTGGTAAATTTCAAAGTAAACTGTGTGGACCGTCAAGAAAAGAGTCTGATTTGCAAAGTTATTTGGATGAACCAAAACTTGACCATAAGAAATATGAAGACTTAGATATCCTTGAATATTGGAAAGCAAATGAAGGGAAATATCCTGAAGTAGCTATGATGGCACGTGATGTTTTGAGCATTCCAATTACCACTGTTGCATCAGAGTCATCGTTCAGTATAGGTGGACGTATAAGTTATGAATAA
Protein sequence

>MS.gene22938.t1

MSNGGDVSDEVESSVPSSKKPKTSVVWEFFEKYLDENGQPRARCLNCATTYAAKNTSGTSNLKSHLLKCVEGGDVSSYPPLDQEKYREKIAQVIVKNSYPFAFVEHDGIEDLHCFLHPHVKGISRNTAKSDVLKLYRKEKENLKCYLRSIPGKICLTSDLWSSINTDQYMVLTAHFVNRRWELEKKVLAFFHFPPPHTGVNLAEKLLSLLRDWGIEKKIFTITLDNASNNDAMVNILKRHPSFGPSLIVDGLYFHVRCGAHVLNLIVHEGIKAVGASLDKIRVCVKYIRGSEARKMKFASCLEQLTQVTSKQLRQDMPIRWNSSYLMLDSAIGLREAFTLLQDIDPHFDNLSDEEWIEAQKIAEFLKPFYDITTLFSGTTYPTANLYFSGVWRIQMKIKEVACVFHSEFENQDQVCCMAKRMKVKFDKYWTSYSVILSFAVILDPRFKLQFVEYCYGKLFGSDGEKLANDILDKLKAFFQEYLKSSKEISGSSSQSSVRGSPQITSSDFADFGKFQSKLCGPSRKESDLQSYLDEPKLDHKKYEDLDILEYWKANEGKYPEVAMMARDVLSIPITTVASESSFSIGGRISYE