Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24592.t1 | XP_013468213.1 | 90.1 | 467 | 39 | 1 | 1 | 467 | 17 | 476 | 6.50E-240 | 839.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24592.t1 | A0A072VVY5 | 90.1 | 467 | 39 | 1 | 1 | 467 | 17 | 476 | 4.7e-240 | 839.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059865 | MS.gene24592 | -0.805539 | 1.27E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24592.t1 | MTR_1g064690 | 91.239 | 468 | 34 | 1 | 1 | 468 | 17 | 477 | 0.0 | 849 |
| MS.gene24592.t1 | MTR_7g101130 | 49.687 | 479 | 188 | 18 | 3 | 457 | 17 | 466 | 1.02e-133 | 394 |
| MS.gene24592.t1 | MTR_4g013290 | 23.557 | 433 | 260 | 14 | 79 | 457 | 94 | 509 | 1.14e-20 | 95.1 |
| MS.gene24592.t1 | MTR_3g031200 | 23.837 | 516 | 300 | 20 | 1 | 456 | 11 | 493 | 3.59e-20 | 93.6 |
| MS.gene24592.t1 | MTR_3g100550 | 23.939 | 330 | 176 | 14 | 145 | 457 | 147 | 418 | 3.09e-13 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24592.t1 | AT3G53400 | 41.614 | 471 | 236 | 13 | 3 | 457 | 17 | 464 | 3.28e-105 | 321 |
| MS.gene24592.t1 | AT5G03190 | 38.854 | 471 | 234 | 15 | 3 | 457 | 17 | 449 | 9.73e-99 | 304 |
| MS.gene24592.t1 | AT5G03190 | 38.771 | 472 | 235 | 15 | 2 | 457 | 40 | 473 | 1.05e-98 | 305 |
| MS.gene24592.t1 | AT1G24480 | 28.177 | 181 | 114 | 5 | 63 | 236 | 62 | 233 | 1.74e-12 | 67.4 |
Find 62 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAAATTCTCATTCAATTT+TGG | 0.100198 | 1.3:+46634927 | None:intergenic |
| CACTTATTTGCCGAAGTTTA+AGG | 0.149063 | 1.3:-46635129 | MS.gene24592:CDS |
| CTTTCAGATATATATGAAAA+AGG | 0.205406 | 1.3:-46634759 | MS.gene24592:CDS |
| TGGAATTGGTGCTTTGCTTT+TGG | 0.223055 | 1.3:-46635456 | MS.gene24592:CDS |
| TTCTTCAAGTAAGAAGAATT+TGG | 0.288457 | 1.3:-46635102 | MS.gene24592:CDS |
| AATTTGGTTTATGTTAATAT+TGG | 0.311724 | 1.3:-46635086 | MS.gene24592:CDS |
| TTGAAGTCTTTCTTATCAAT+TGG | 0.335179 | 1.3:+46635026 | None:intergenic |
| GAACGTATTCTTAAACCTAA+TGG | 0.351263 | 1.3:-46635476 | MS.gene24592:CDS |
| GCAGTCATGATCATAGGTTT+TGG | 0.351442 | 1.3:+46635889 | None:intergenic |
| ATTCTTAAACCTAATGGAAT+TGG | 0.357672 | 1.3:-46635470 | MS.gene24592:CDS |
| GAAGCAGAAACTCTTTGTAT+TGG | 0.379951 | 1.3:-46635692 | MS.gene24592:CDS |
| AGAGGACAAAGAGTTTGATT+TGG | 0.392534 | 1.3:-46634865 | MS.gene24592:CDS |
| TTTCGCAGCAACGCTGAAAT+CGG | 0.404649 | 1.3:+46635368 | None:intergenic |
| GATGATGCAGTCATGATCAT+AGG | 0.409066 | 1.3:+46635883 | None:intergenic |
| AGCAAAGCACCAATTCCATT+AGG | 0.409665 | 1.3:+46635461 | None:intergenic |
| ATGGTTAAGATGAATATTGC+AGG | 0.417602 | 1.3:-46634801 | MS.gene24592:CDS |
| TAAGAATACGTTCAACTTCA+AGG | 0.424190 | 1.3:+46635486 | None:intergenic |
| AGTGTTGTCAAAGAACTAAA+AGG | 0.425355 | 1.3:-46635734 | MS.gene24592:CDS |
| TTTCTTAGCTGCTCAGATAG+TGG | 0.431797 | 1.3:-46634711 | MS.gene24592:CDS |
| GAAGAAACATCCTTAAACTT+CGG | 0.466732 | 1.3:+46635119 | None:intergenic |
| AAGAGTTTGATTTGGTTGCT+TGG | 0.475847 | 1.3:-46634857 | MS.gene24592:CDS |
| GTTAGTCTTGTCAATAATCA+TGG | 0.484833 | 1.3:-46635329 | MS.gene24592:CDS |
| AGTGAAGGAAACACCATATG+AGG | 0.490263 | 1.3:-46635159 | MS.gene24592:CDS |
| GTTTCCACACTGAAGAAACT+TGG | 0.492186 | 1.3:-46635650 | MS.gene24592:CDS |
| GTGAAGATGAGGTTGGTGAT+TGG | 0.495931 | 1.3:-46635064 | MS.gene24592:CDS |
| ACTTCAAGGACAACCAATGC+AGG | 0.500181 | 1.3:+46635500 | None:intergenic |
| AGCTCTTTGTTCTATCATCA+AGG | 0.501727 | 1.3:-46635248 | MS.gene24592:CDS |
| TTATCAATTGGATAAGATTG+TGG | 0.505859 | 1.3:+46635038 | None:intergenic |
| GATGAAACTAAGGTTAATGT+GGG | 0.515313 | 1.3:-46634903 | MS.gene24592:CDS |
| AAAAGTTGCTGTCCCTGCAT+TGG | 0.518228 | 1.3:-46635513 | MS.gene24592:CDS |
| GTGGAGATGGTGAAAGAGAG+AGG | 0.519129 | 1.3:-46634692 | MS.gene24592:CDS |
| CTCTGGAACAAATAAGAACC+TGG | 0.520480 | 1.3:+46635827 | None:intergenic |
| TACAAGGGTCTTAAAAGCAA+TGG | 0.520701 | 1.3:-46634657 | MS.gene24592:CDS |
| GGATGAAACTAAGGTTAATG+TGG | 0.526389 | 1.3:-46634904 | MS.gene24592:CDS |
| ATGGTGTCTTTGTTCATCAA+TGG | 0.529795 | 1.3:-46634638 | MS.gene24592:CDS |
| TGGTGAAAGAGAGAGGTGCA+TGG | 0.537641 | 1.3:-46634685 | MS.gene24592:CDS |
| ATTATGTTGTCGCACGTGAA+AGG | 0.538596 | 1.3:-46634975 | MS.gene24592:CDS |
| AGCTGCTCAGATAGTGGAGA+TGG | 0.559669 | 1.3:-46634705 | MS.gene24592:CDS |
| AATCGAGCCGCCACTAGTGA+AGG | 0.565391 | 1.3:-46635174 | MS.gene24592:CDS |
| GAAACCAAGTTTCTTCAGTG+TGG | 0.567718 | 1.3:+46635646 | None:intergenic |
| GGTGTTTCCTTCACTAGTGG+CGG | 0.572347 | 1.3:+46635167 | None:intergenic |
| AACTGTTTAAGACTCTACTC+TGG | 0.577491 | 1.3:+46635810 | None:intergenic |
| TATGGTGTTTCCTTCACTAG+TGG | 0.578954 | 1.3:+46635164 | None:intergenic |
| TCTTCACTCACTACCTACCA+TGG | 0.580333 | 1.3:-46635921 | MS.gene24592:CDS |
| TGCTTGGTTTAAAGAAGCTG+TGG | 0.582519 | 1.3:-46634841 | MS.gene24592:CDS |
| GAGGTGCATGGATATTTACA+AGG | 0.586229 | 1.3:-46634673 | MS.gene24592:CDS |
| TCAAACCTCACTTTAACACC+AGG | 0.590536 | 1.3:-46635845 | MS.gene24592:CDS |
| TGAGTGAAGAAGAGAAACGA+TGG | 0.592739 | 1.3:+46635934 | None:intergenic |
| AATATTGGTGAAGATGAGGT+TGG | 0.595070 | 1.3:-46635071 | MS.gene24592:CDS |
| AAGAACCTGGTGTTAAAGTG+AGG | 0.599372 | 1.3:+46635840 | None:intergenic |
| GTGTCTTTGTTCATCAATGG+TGG | 0.604469 | 1.3:-46634635 | MS.gene24592:CDS |
| TTGGGAGCTAAATCCATGGT+AGG | 0.606124 | 1.3:+46635908 | None:intergenic |
| TGTTAATATTGGTGAAGATG+AGG | 0.620594 | 1.3:-46635075 | MS.gene24592:CDS |
| TGAAACTAAGGTTAATGTGG+GGG | 0.625759 | 1.3:-46634901 | MS.gene24592:CDS |
| TCAAAGAACTAAAAGGTGAA+CGG | 0.636645 | 1.3:-46635727 | MS.gene24592:CDS |
| ATGAAACTAAGGTTAATGTG+GGG | 0.644701 | 1.3:-46634902 | MS.gene24592:CDS |
| CTTCAAGGACAACCAATGCA+GGG | 0.669621 | 1.3:+46635501 | None:intergenic |
| AGAAACGATGGAAATAGCAG+AGG | 0.677899 | 1.3:+46635946 | None:intergenic |
| AGGTGCATGGATATTTACAA+GGG | 0.710220 | 1.3:-46634672 | MS.gene24592:CDS |
| CAAAGTCGAACGATTCATCG+TGG | 0.733200 | 1.3:+46635555 | None:intergenic |
| TGTTGAATGAGAAAACACGA+TGG | 0.736414 | 1.3:+46635609 | None:intergenic |
| TGATAAATAAATGTAACACG+CGG | 0.745811 | 1.3:+46634951 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCAGATATATATGAAAAA+GGG | - | chr1.3:46635824-46635843 | MS.gene24592:CDS | 15.0% |
| !!! | AATTTGGTTTATGTTAATAT+TGG | - | chr1.3:46635496-46635515 | MS.gene24592:CDS | 15.0% |
| !! | CTTTCAGATATATATGAAAA+AGG | - | chr1.3:46635823-46635842 | MS.gene24592:CDS | 20.0% |
| !!! | AAAATTGAATGAGAATTTTG+AGG | - | chr1.3:46635657-46635676 | MS.gene24592:CDS | 20.0% |
| !!! | ATTTTTTCTTGAAAACAACA+AGG | + | chr1.3:46635279-46635298 | None:intergenic | 20.0% |
| !!! | TCAAAATTCTCATTCAATTT+TGG | + | chr1.3:46635658-46635677 | None:intergenic | 20.0% |
| ! | ATTCTTAAACCTAATGGAAT+TGG | - | chr1.3:46635112-46635131 | MS.gene24592:CDS | 25.0% |
| ! | TGATAAATAAATGTAACACG+CGG | + | chr1.3:46635634-46635653 | None:intergenic | 25.0% |
| ! | TTATCAATTGGATAAGATTG+TGG | + | chr1.3:46635547-46635566 | None:intergenic | 25.0% |
| ! | TTCTTCAAGTAAGAAGAATT+TGG | - | chr1.3:46635480-46635499 | MS.gene24592:CDS | 25.0% |
| ! | TTGAAGTCTTTCTTATCAAT+TGG | + | chr1.3:46635559-46635578 | None:intergenic | 25.0% |
| ! | TTGCAAAAAAGAAATCAACT+TGG | - | chr1.3:46634820-46634839 | MS.gene24592:CDS | 25.0% |
| !! | TAGAGTCTTAAACAGTTTTT+GGG | - | chr1.3:46634778-46634797 | MS.gene24592:CDS | 25.0% |
| !! | TTAACACTTTTACAATCTTC+TGG | + | chr1.3:46635364-46635383 | None:intergenic | 25.0% |
| !!! | TAAGTGATTTTTTCCTCATA+TGG | + | chr1.3:46635439-46635458 | None:intergenic | 25.0% |
| !!! | TTTGTTTTGTCTAAGGATTT+GGG | - | chr1.3:46635046-46635065 | MS.gene24592:CDS | 25.0% |
| AGTGTTGTCAAAGAACTAAA+AGG | - | chr1.3:46634848-46634867 | MS.gene24592:CDS | 30.0% | |
| ATGAAACTAAGGTTAATGTG+GGG | - | chr1.3:46635680-46635699 | MS.gene24592:CDS | 30.0% | |
| ATGGTTAAGATGAATATTGC+AGG | - | chr1.3:46635781-46635800 | MS.gene24592:CDS | 30.0% | |
| GAACGTATTCTTAAACCTAA+TGG | - | chr1.3:46635106-46635125 | MS.gene24592:CDS | 30.0% | |
| GAAGAAACATCCTTAAACTT+CGG | + | chr1.3:46635466-46635485 | None:intergenic | 30.0% | |
| GATGAAACTAAGGTTAATGT+GGG | - | chr1.3:46635679-46635698 | MS.gene24592:CDS | 30.0% | |
| TAAGAATACGTTCAACTTCA+AGG | + | chr1.3:46635099-46635118 | None:intergenic | 30.0% | |
| TCAAAGAACTAAAAGGTGAA+CGG | - | chr1.3:46634855-46634874 | MS.gene24592:CDS | 30.0% | |
| TGTTAATATTGGTGAAGATG+AGG | - | chr1.3:46635507-46635526 | MS.gene24592:CDS | 30.0% | |
| ! | AGAGTCTTAAACAGTTTTTG+GGG | - | chr1.3:46634779-46634798 | MS.gene24592:CDS | 30.0% |
| ! | GTAGAGTCTTAAACAGTTTT+TGG | - | chr1.3:46634777-46634796 | MS.gene24592:CDS | 30.0% |
| !! | GAATTTTGAGGATGAAACTA+AGG | - | chr1.3:46635669-46635688 | MS.gene24592:CDS | 30.0% |
| !! | GTTAGTCTTGTCAATAATCA+TGG | - | chr1.3:46635253-46635272 | MS.gene24592:CDS | 30.0% |
| !!! | CTTTGTTTTGTCTAAGGATT+TGG | - | chr1.3:46635045-46635064 | MS.gene24592:CDS | 30.0% |
| AATATTGGTGAAGATGAGGT+TGG | - | chr1.3:46635511-46635530 | MS.gene24592:CDS | 35.0% | |
| AGAGGACAAAGAGTTTGATT+TGG | - | chr1.3:46635717-46635736 | MS.gene24592:CDS | 35.0% | |
| AGCTCTTTGTTCTATCATCA+AGG | - | chr1.3:46635334-46635353 | MS.gene24592:CDS | 35.0% | |
| AGGTGCATGGATATTTACAA+GGG | - | chr1.3:46635910-46635929 | MS.gene24592:CDS | 35.0% | |
| ATGGTGTCTTTGTTCATCAA+TGG | - | chr1.3:46635944-46635963 | MS.gene24592:CDS | 35.0% | |
| CACTTATTTGCCGAAGTTTA+AGG | - | chr1.3:46635453-46635472 | MS.gene24592:CDS | 35.0% | |
| GAAGCAGAAACTCTTTGTAT+TGG | - | chr1.3:46634890-46634909 | MS.gene24592:CDS | 35.0% | |
| GGATGAAACTAAGGTTAATG+TGG | - | chr1.3:46635678-46635697 | MS.gene24592:CDS | 35.0% | |
| TACAAGGGTCTTAAAAGCAA+TGG | - | chr1.3:46635925-46635944 | MS.gene24592:CDS | 35.0% | |
| TGAAACTAAGGTTAATGTGG+GGG | - | chr1.3:46635681-46635700 | MS.gene24592:CDS | 35.0% | |
| TGTTGAATGAGAAAACACGA+TGG | + | chr1.3:46634976-46634995 | None:intergenic | 35.0% | |
| ! | AACTGTTTAAGACTCTACTC+TGG | + | chr1.3:46634775-46634794 | None:intergenic | 35.0% |
| ! | CAGTCATGATCATAGGTTTT+GGG | + | chr1.3:46634695-46634714 | None:intergenic | 35.0% |
| !! | AAGAGTTTGATTTGGTTGCT+TGG | - | chr1.3:46635725-46635744 | MS.gene24592:CDS | 35.0% |
| !!! | GGAGAATGCTGATTTTGTTA+TGG | - | chr1.3:46635762-46635781 | MS.gene24592:CDS | 35.0% |
| !!! | GTTCGACTTTGTTTTGTCTA+AGG | - | chr1.3:46635039-46635058 | MS.gene24592:CDS | 35.0% |
| AGAAACGATGGAAATAGCAG+AGG | + | chr1.3:46634639-46634658 | None:intergenic | 40.0% | |
| AGTGAAGGAAACACCATATG+AGG | - | chr1.3:46635423-46635442 | MS.gene24592:CDS | 40.0% | |
| ATTATGTTGTCGCACGTGAA+AGG | - | chr1.3:46635607-46635626 | MS.gene24592:CDS | 40.0% | |
| GAAACCAAGTTTCTTCAGTG+TGG | + | chr1.3:46634939-46634958 | None:intergenic | 40.0% | |
| GAGGTGCATGGATATTTACA+AGG | - | chr1.3:46635909-46635928 | MS.gene24592:CDS | 40.0% | |
| GATGATGCAGTCATGATCAT+AGG | + | chr1.3:46634702-46634721 | None:intergenic | 40.0% | |
| GTGTCTTTGTTCATCAATGG+TGG | - | chr1.3:46635947-46635966 | MS.gene24592:CDS | 40.0% | |
| GTTTCCACACTGAAGAAACT+TGG | - | chr1.3:46634932-46634951 | MS.gene24592:CDS | 40.0% | |
| TATGGTGTTTCCTTCACTAG+TGG | + | chr1.3:46635421-46635440 | None:intergenic | 40.0% | |
| TCAAACCTCACTTTAACACC+AGG | - | chr1.3:46634737-46634756 | MS.gene24592:CDS | 40.0% | |
| TGAGTGAAGAAGAGAAACGA+TGG | + | chr1.3:46634651-46634670 | None:intergenic | 40.0% | |
| TGCTTGGTTTAAAGAAGCTG+TGG | - | chr1.3:46635741-46635760 | MS.gene24592:CDS | 40.0% | |
| TTTCTTAGCTGCTCAGATAG+TGG | - | chr1.3:46635871-46635890 | MS.gene24592:CDS | 40.0% | |
| ! | AAGAACCTGGTGTTAAAGTG+AGG | + | chr1.3:46634745-46634764 | None:intergenic | 40.0% |
| ! | AGCAAAGCACCAATTCCATT+AGG | + | chr1.3:46635124-46635143 | None:intergenic | 40.0% |
| ! | GCAGTCATGATCATAGGTTT+TGG | + | chr1.3:46634696-46634715 | None:intergenic | 40.0% |
| !! | CTCTGGAACAAATAAGAACC+TGG | + | chr1.3:46634758-46634777 | None:intergenic | 40.0% |
| !! | TGGAATTGGTGCTTTGCTTT+TGG | - | chr1.3:46635126-46635145 | MS.gene24592:CDS | 40.0% |
| AAAAGTTGCTGTCCCTGCAT+TGG | - | chr1.3:46635069-46635088 | MS.gene24592:CDS | 45.0% | |
| ACTTCAAGGACAACCAATGC+AGG | + | chr1.3:46635085-46635104 | None:intergenic | 45.0% | |
| CAAAGTCGAACGATTCATCG+TGG | + | chr1.3:46635030-46635049 | None:intergenic | 45.0% | |
| CTTCAAGGACAACCAATGCA+GGG | + | chr1.3:46635084-46635103 | None:intergenic | 45.0% | |
| TCTTCACTCACTACCTACCA+TGG | - | chr1.3:46634661-46634680 | MS.gene24592:CDS | 45.0% | |
| TTGGGAGCTAAATCCATGGT+AGG | + | chr1.3:46634677-46634696 | None:intergenic | 45.0% | |
| TTTCGCAGCAACGCTGAAAT+CGG | + | chr1.3:46635217-46635236 | None:intergenic | 45.0% | |
| !! | GTGAAGATGAGGTTGGTGAT+TGG | - | chr1.3:46635518-46635537 | MS.gene24592:CDS | 45.0% |
| !!! | GGTTTTGGGAGCTAAATCCA+TGG | + | chr1.3:46634681-46634700 | None:intergenic | 45.0% |
| GGTGTTTCCTTCACTAGTGG+CGG | + | chr1.3:46635418-46635437 | None:intergenic | 50.0% | |
| GTGGAGATGGTGAAAGAGAG+AGG | - | chr1.3:46635890-46635909 | MS.gene24592:CDS | 50.0% | |
| TAATGTGGGGGATGCAGATG+TGG | - | chr1.3:46635693-46635712 | MS.gene24592:CDS | 50.0% | |
| ! | TGGTGAAAGAGAGAGGTGCA+TGG | - | chr1.3:46635897-46635916 | MS.gene24592:CDS | 50.0% |
| !! | AGCTGCTCAGATAGTGGAGA+TGG | - | chr1.3:46635877-46635896 | MS.gene24592:CDS | 50.0% |
| AATCGAGCCGCCACTAGTGA+AGG | - | chr1.3:46635408-46635427 | MS.gene24592:CDS | 55.0% | |
| GGGGGATGCAGATGTGGAAG+AGG | - | chr1.3:46635699-46635718 | MS.gene24592:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 46634599 | 46636005 | 46634599 | ID=MS.gene24592 |
| chr1.3 | mRNA | 46634599 | 46636005 | 46634599 | ID=MS.gene24592.t1;Parent=MS.gene24592 |
| chr1.3 | exon | 46634599 | 46636005 | 46634599 | ID=MS.gene24592.t1.exon1;Parent=MS.gene24592.t1 |
| chr1.3 | CDS | 46634599 | 46636005 | 46634599 | ID=cds.MS.gene24592.t1;Parent=MS.gene24592.t1 |
| Gene Sequence |
| Protein sequence |
