Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24800.t1 | XP_003609992.1 | 97.9 | 190 | 4 | 0 | 1 | 190 | 1 | 190 | 1.90E-105 | 391.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24800.t1 | G7JT65 | 97.9 | 190 | 4 | 0 | 1 | 190 | 1 | 190 | 1.4e-105 | 391.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene24800 | 0.81242 | 4.28E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24800.t1 | MTR_4g125100 | 97.895 | 190 | 4 | 0 | 1 | 190 | 1 | 190 | 4.06e-139 | 385 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24800.t1 | AT1G19130 | 68.254 | 189 | 57 | 3 | 3 | 190 | 6 | 192 | 4.42e-90 | 262 |
Find 38 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGTTAAATTTACATGTCT+TGG | 0.348435 | 8.2:+7578983 | MS.gene24800:CDS |
| TCCACGTAAAATCCACCTTC+TGG | 0.366637 | 8.2:-7577951 | None:intergenic |
| GAAACTCATTACTCTCTTGT+AGG | 0.414889 | 8.2:+7579139 | MS.gene24800:CDS |
| ATATACTTTCTGCTTCCATC+TGG | 0.431150 | 8.2:+7578135 | MS.gene24800:CDS |
| ACACGGTGCCTCCGAATGTC+TGG | 0.437212 | 8.2:+7579038 | MS.gene24800:CDS |
| GTAGGATGCACTTGTGCACC+TGG | 0.437438 | 8.2:+7579157 | MS.gene24800:CDS |
| GTGCCTCCGAATGTCTGGTT+TGG | 0.451216 | 8.2:+7579043 | MS.gene24800:CDS |
| AACAAAGGAATTACGGTGAT+AGG | 0.454551 | 8.2:-7578219 | None:intergenic |
| GTTTAGCAAGTTCAAAATCC+TGG | 0.459347 | 8.2:-7579189 | None:intergenic |
| ATCAGAAAGAATGTCTTTGT+CGG | 0.473073 | 8.2:+7577890 | None:intergenic |
| TGATGCCATGTTTCAGAAGA+AGG | 0.497508 | 8.2:-7578183 | None:intergenic |
| AAAGAACCAAACCAGACATT+CGG | 0.516467 | 8.2:-7579049 | None:intergenic |
| GAAACATGGCATCATTACAT+TGG | 0.519533 | 8.2:+7578192 | MS.gene24800:CDS |
| TCTTGGATCTGATCTAACTA+AGG | 0.525817 | 8.2:+7579000 | MS.gene24800:CDS |
| CAGAAGAAGGAATATGATGA+AGG | 0.540545 | 8.2:-7578170 | None:intergenic |
| TAAAATCCACCTTCTGGATG+AGG | 0.547021 | 8.2:-7577945 | None:intergenic |
| TTTACTTACATTCAGGTGGA+AGG | 0.547646 | 8.2:-7578013 | None:intergenic |
| TTTGCTGTCTCTGCAGATGG+GGG | 0.555085 | 8.2:+7579085 | MS.gene24800:CDS |
| AAGTATATGCAGGTCGACAC+TGG | 0.558993 | 8.2:-7578120 | None:intergenic |
| ATATTCCTTCTTCTGAAACA+TGG | 0.566858 | 8.2:+7578178 | MS.gene24800:CDS |
| GTGACAAGAGACTCCAATTG+AGG | 0.568370 | 8.2:-7579235 | None:intergenic |
| TTGCTGTCTCTGCAGATGGG+GGG | 0.568555 | 8.2:+7579086 | MS.gene24800:CDS |
| TGCAGAGACAGCAAAATCAC+TGG | 0.576800 | 8.2:-7579077 | None:intergenic |
| ATTGAGGAAAACGTGAAATG+AGG | 0.583631 | 8.2:-7579219 | None:intergenic |
| TGGAAGCAGAAAGTATATGC+AGG | 0.591339 | 8.2:-7578130 | None:intergenic |
| TTCGGAGGCACCGTGTATTG+CGG | 0.594043 | 8.2:-7579031 | None:intergenic |
| AGCGAGCAGCAACATTACTC+AGG | 0.596950 | 8.2:-7579271 | None:intergenic |
| CTGCAACCTCATCCAGAAGG+TGG | 0.598332 | 8.2:+7577939 | MS.gene24800:CDS |
| GTGAAGGGTTCAGAAAGAGA+TGG | 0.604440 | 8.2:+7579115 | MS.gene24800:CDS |
| AAATCCTGGAACTGAAAACC+AGG | 0.610826 | 8.2:-7579175 | None:intergenic |
| GGATCAGACACCGCAATACA+CGG | 0.615581 | 8.2:+7579021 | MS.gene24800:CDS |
| AGGCGAGACATGGTACCAGA+TGG | 0.617797 | 8.2:-7578150 | None:intergenic |
| AATATGATGAAGGCGAGACA+TGG | 0.618034 | 8.2:-7578160 | None:intergenic |
| GAGACAGCAAAATCACTGGT+CGG | 0.647906 | 8.2:-7579073 | None:intergenic |
| AATCTGCAACCTCATCCAGA+AGG | 0.653089 | 8.2:+7577936 | MS.gene24800:CDS |
| ATATGCAGGTCGACACTGGT+CGG | 0.655426 | 8.2:-7578116 | None:intergenic |
| AAGTAGAAAGTTGAACAGAG+TGG | 0.677760 | 8.2:-7577986 | None:intergenic |
| GAACCAAACCAGACATTCGG+AGG | 0.677908 | 8.2:-7579046 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAATATGATTGATAAATA+AGG | - | chr8.2:7578529-7578548 | None:intergenic | 10.0% |
| !! | AAAAAAAAACAAAGGAATTA+CGG | - | chr8.2:7578229-7578248 | None:intergenic | 15.0% |
| !! | ATTACAAGATTAATTATATG+TGG | + | chr8.2:7578285-7578304 | MS.gene24800:intron | 15.0% |
| !! | GAAAAAGAAAAAAAAAACAA+AGG | - | chr8.2:7578237-7578256 | None:intergenic | 15.0% |
| !! | TTTGTTAGTATAATAATACT+TGG | + | chr8.2:7578916-7578935 | MS.gene24800:intron | 15.0% |
| !! | AGATTGTTAATTCAGTATAT+GGG | + | chr8.2:7578377-7578396 | MS.gene24800:intron | 20.0% |
| !! | TAGATTGTTAATTCAGTATA+TGG | + | chr8.2:7578376-7578395 | MS.gene24800:intron | 20.0% |
| !!! | ATTTGATTTTACTTACATTC+AGG | - | chr8.2:7578023-7578042 | None:intergenic | 20.0% |
| !!! | CATTAGATTTGAAACATATT+GGG | + | chr8.2:7578625-7578644 | MS.gene24800:intron | 20.0% |
| !!! | TCATTAGATTTGAAACATAT+TGG | + | chr8.2:7578624-7578643 | MS.gene24800:intron | 20.0% |
| ! | AGTGTTAAATTTACATGTCT+TGG | + | chr8.2:7578983-7579002 | MS.gene24800:CDS | 25.0% |
| ! | TGATGAAATTGAAAAGAAAG+AGG | - | chr8.2:7578052-7578071 | None:intergenic | 25.0% |
| !!! | GGGGTTTTGAAATTTAATTT+TGG | + | chr8.2:7578652-7578671 | MS.gene24800:intron | 25.0% |
| GTTGAATTAACCAAAAACTG+TGG | - | chr8.2:7578790-7578809 | None:intergenic | 30.0% | |
| TTTGCATGTGTTGGAAAATT+GGG | + | chr8.2:7578878-7578897 | MS.gene24800:intron | 30.0% | |
| ! | ATTTGAAACATATTGGGAGT+TGG | + | chr8.2:7578631-7578650 | MS.gene24800:intron | 30.0% |
| ! | TGATTTTACTTACATTCAGG+TGG | - | chr8.2:7578020-7578039 | None:intergenic | 30.0% |
| ! | TTTGAAACATATTGGGAGTT+GGG | + | chr8.2:7578632-7578651 | MS.gene24800:intron | 30.0% |
| ! | TTTTGCATGTGTTGGAAAAT+TGG | + | chr8.2:7578877-7578896 | MS.gene24800:intron | 30.0% |
| !! | ATATTCCTTCTTCTGAAACA+TGG | + | chr8.2:7578178-7578197 | MS.gene24800:CDS | 30.0% |
| !!! | ATTTGTTTTATTGTAGGTGG+TGG | + | chr8.2:7578940-7578959 | MS.gene24800:intron | 30.0% |
| !!! | CTTGGCATTTGTTTTATTGT+AGG | + | chr8.2:7578934-7578953 | MS.gene24800:intron | 30.0% |
| AAAGAACCAAACCAGACATT+CGG | - | chr8.2:7579052-7579071 | None:intergenic | 35.0% | |
| AACAAAGGAATTACGGTGAT+AGG | - | chr8.2:7578222-7578241 | None:intergenic | 35.0% | |
| AAGTAGAAAGTTGAACAGAG+TGG | - | chr8.2:7577989-7578008 | None:intergenic | 35.0% | |
| AGCAATACACTTTGTAGCAA+CGG | - | chr8.2:7578454-7578473 | None:intergenic | 35.0% | |
| ATATACTTTCTGCTTCCATC+TGG | + | chr8.2:7578135-7578154 | MS.gene24800:CDS | 35.0% | |
| ATTGAGGAAAACGTGAAATG+AGG | - | chr8.2:7579222-7579241 | None:intergenic | 35.0% | |
| CAGAAGAAGGAATATGATGA+AGG | - | chr8.2:7578173-7578192 | None:intergenic | 35.0% | |
| CGAAAAGATTTGAGCAAACA+TGG | + | chr8.2:7578675-7578694 | MS.gene24800:intron | 35.0% | |
| GAAACATGGCATCATTACAT+TGG | + | chr8.2:7578192-7578211 | MS.gene24800:CDS | 35.0% | |
| GAAACTCATTACTCTCTTGT+AGG | + | chr8.2:7579139-7579158 | MS.gene24800:CDS | 35.0% | |
| GGAACTGTATTAGACGATTT+CGG | - | chr8.2:7578701-7578720 | None:intergenic | 35.0% | |
| GTTCTTTAATGTGATCAACC+AGG | - | chr8.2:7578334-7578353 | None:intergenic | 35.0% | |
| GTTTAGCAAGTTCAAAATCC+TGG | - | chr8.2:7579192-7579211 | None:intergenic | 35.0% | |
| TCTTGGATCTGATCTAACTA+AGG | + | chr8.2:7579000-7579019 | MS.gene24800:CDS | 35.0% | |
| TTCCAACACATGCAAAATTG+TGG | - | chr8.2:7578874-7578893 | None:intergenic | 35.0% | |
| TTGAAACATATTGGGAGTTG+GGG | + | chr8.2:7578633-7578652 | MS.gene24800:intron | 35.0% | |
| TTTACTTACATTCAGGTGGA+AGG | - | chr8.2:7578016-7578035 | None:intergenic | 35.0% | |
| TTTGCTCCGTAAATACTGAT+TGG | + | chr8.2:7578550-7578569 | MS.gene24800:intron | 35.0% | |
| ! | CATTTCACGTTTTCCTCAAT+TGG | + | chr8.2:7579222-7579241 | MS.gene24800:CDS | 35.0% |
| ! | TTGCTCCGTAAATACTGATT+GGG | + | chr8.2:7578551-7578570 | MS.gene24800:intron | 35.0% |
| !!! | GGCATTTGTTTTATTGTAGG+TGG | + | chr8.2:7578937-7578956 | MS.gene24800:intron | 35.0% |
| AAACTAACTCACTACGCAAG+AGG | - | chr8.2:7578477-7578496 | None:intergenic | 40.0% | |
| AAATCCTGGAACTGAAAACC+AGG | - | chr8.2:7579178-7579197 | None:intergenic | 40.0% | |
| AATATGATGAAGGCGAGACA+TGG | - | chr8.2:7578163-7578182 | None:intergenic | 40.0% | |
| ATCACATCTATAGCTGCATC+AGG | - | chr8.2:7578722-7578741 | None:intergenic | 40.0% | |
| CACCTCCCAATCAGTATTTA+CGG | - | chr8.2:7578559-7578578 | None:intergenic | 40.0% | |
| GATTGCAATAGTTGCGATTG+TGG | + | chr8.2:7578422-7578441 | MS.gene24800:intron | 40.0% | |
| TAAAATCCACCTTCTGGATG+AGG | - | chr8.2:7577948-7577967 | None:intergenic | 40.0% | |
| TGATGCCATGTTTCAGAAGA+AGG | - | chr8.2:7578186-7578205 | None:intergenic | 40.0% | |
| ! | AGGTGTCGCAAGACAAAAAT+GGG | - | chr8.2:7578314-7578333 | None:intergenic | 40.0% |
| ! | GACCACAATTTTGCATGTGT+TGG | + | chr8.2:7578869-7578888 | MS.gene24800:intron | 40.0% |
| ! | TGGAAGCAGAAAGTATATGC+AGG | - | chr8.2:7578133-7578152 | None:intergenic | 40.0% |
| !! | ATTTTGCTGTCTCTGCAGAT+GGG | + | chr8.2:7579083-7579102 | MS.gene24800:CDS | 40.0% |
| AAGTATATGCAGGTCGACAC+TGG | - | chr8.2:7578123-7578142 | None:intergenic | 45.0% | |
| AATCTGCAACCTCATCCAGA+AGG | + | chr8.2:7577936-7577955 | MS.gene24800:CDS | 45.0% | |
| GAGACAGCAAAATCACTGGT+CGG | - | chr8.2:7579076-7579095 | None:intergenic | 45.0% | |
| GATTGAAGATCACACTGCAG+TGG | + | chr8.2:7578739-7578758 | MS.gene24800:intron | 45.0% | |
| GTGAAGGGTTCAGAAAGAGA+TGG | + | chr8.2:7579115-7579134 | MS.gene24800:CDS | 45.0% | |
| GTGACAAGAGACTCCAATTG+AGG | - | chr8.2:7579238-7579257 | None:intergenic | 45.0% | |
| TCCACGTAAAATCCACCTTC+TGG | - | chr8.2:7577954-7577973 | None:intergenic | 45.0% | |
| TGCAGAGACAGCAAAATCAC+TGG | - | chr8.2:7579080-7579099 | None:intergenic | 45.0% | |
| ! | CAGGTGTCGCAAGACAAAAA+TGG | - | chr8.2:7578315-7578334 | None:intergenic | 45.0% |
| ! | CATTTTTGTCTTGCGACACC+TGG | + | chr8.2:7578313-7578332 | MS.gene24800:intron | 45.0% |
| ! | CTCCGTAAATACTGATTGGG+AGG | + | chr8.2:7578554-7578573 | MS.gene24800:intron | 45.0% |
| !! | GATTTTGCTGTCTCTGCAGA+TGG | + | chr8.2:7579082-7579101 | MS.gene24800:CDS | 45.0% |
| !! | TCCAGAAGGTGGATTTTACG+TGG | + | chr8.2:7577950-7577969 | MS.gene24800:CDS | 45.0% |
| !! | TTTTGCTGTCTCTGCAGATG+GGG | + | chr8.2:7579084-7579103 | MS.gene24800:CDS | 45.0% |
| AGATGGGGGGTCATTTGTGA+AGG | + | chr8.2:7579099-7579118 | MS.gene24800:CDS | 50.0% | |
| ATATGCAGGTCGACACTGGT+CGG | - | chr8.2:7578119-7578138 | None:intergenic | 50.0% | |
| GAACCAAACCAGACATTCGG+AGG | - | chr8.2:7579049-7579068 | None:intergenic | 50.0% | |
| GATGGGGGGTCATTTGTGAA+GGG | + | chr8.2:7579100-7579119 | MS.gene24800:CDS | 50.0% | |
| GGATCAGACACCGCAATACA+CGG | + | chr8.2:7579021-7579040 | MS.gene24800:CDS | 50.0% | |
| TGAAGATCACACTGCAGTGG+CGG | + | chr8.2:7578742-7578761 | MS.gene24800:intron | 50.0% | |
| ! | GCAGAGGCTACCACAGTTTT+TGG | + | chr8.2:7578777-7578796 | MS.gene24800:intron | 50.0% |
| ! | TTTGCTGTCTCTGCAGATGG+GGG | + | chr8.2:7579085-7579104 | MS.gene24800:CDS | 50.0% |
| !! | TGCACCTGGTTTTCAGTTCC+AGG | + | chr8.2:7579171-7579190 | MS.gene24800:CDS | 50.0% |
| AGGCGAGACATGGTACCAGA+TGG | - | chr8.2:7578153-7578172 | None:intergenic | 55.0% | |
| CTGCAACCTCATCCAGAAGG+TGG | + | chr8.2:7577939-7577958 | MS.gene24800:CDS | 55.0% | |
| GTAGGATGCACTTGTGCACC+TGG | + | chr8.2:7579157-7579176 | MS.gene24800:CDS | 55.0% | |
| GTGCCTCCGAATGTCTGGTT+TGG | + | chr8.2:7579043-7579062 | MS.gene24800:CDS | 55.0% | |
| TTGCTGTCTCTGCAGATGGG+GGG | + | chr8.2:7579086-7579105 | MS.gene24800:CDS | 55.0% | |
| !! | TTCGGAGGCACCGTGTATTG+CGG | - | chr8.2:7579034-7579053 | None:intergenic | 55.0% |
| ACACGGTGCCTCCGAATGTC+TGG | + | chr8.2:7579038-7579057 | MS.gene24800:CDS | 60.0% | |
| GCGGTGCAATGCTGTTGCAG+AGG | + | chr8.2:7578761-7578780 | MS.gene24800:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7577900 | 7579279 | 7577900 | ID=MS.gene24800 |
| chr8.2 | mRNA | 7577900 | 7579279 | 7577900 | ID=MS.gene24800.t1;Parent=MS.gene24800 |
| chr8.2 | exon | 7577900 | 7578026 | 7577900 | ID=MS.gene24800.t1.exon1;Parent=MS.gene24800.t1 |
| chr8.2 | CDS | 7577900 | 7578026 | 7577900 | ID=cds.MS.gene24800.t1;Parent=MS.gene24800.t1 |
| chr8.2 | exon | 7578106 | 7578227 | 7578106 | ID=MS.gene24800.t1.exon2;Parent=MS.gene24800.t1 |
| chr8.2 | CDS | 7578106 | 7578227 | 7578106 | ID=cds.MS.gene24800.t1;Parent=MS.gene24800.t1 |
| chr8.2 | exon | 7578956 | 7579279 | 7578956 | ID=MS.gene24800.t1.exon3;Parent=MS.gene24800.t1 |
| chr8.2 | CDS | 7578956 | 7579279 | 7578956 | ID=cds.MS.gene24800.t1;Parent=MS.gene24800.t1 |
| Gene Sequence |
| Protein sequence |
