Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24837.t1 | XP_013458429.1 | 94.8 | 347 | 13 | 2 | 1 | 346 | 1 | 343 | 9.30E-135 | 490 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24837.t1 | Q9M2B3 | 37.8 | 217 | 95 | 7 | 152 | 345 | 140 | 339 | 1.4e-22 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24837.t1 | A0A072V2W4 | 94.8 | 347 | 13 | 2 | 1 | 346 | 1 | 343 | 6.7e-135 | 490.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053536 | MS.gene24837 | 0.814801 | 1.28E-51 | -1.69E-46 |
| MS.gene053538 | MS.gene24837 | 0.808439 | 3.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24837.t1 | MTR_4g125620 | 94.236 | 347 | 15 | 2 | 1 | 346 | 1 | 343 | 0.0 | 599 |
| MS.gene24837.t1 | MTR_8g019660 | 32.518 | 409 | 197 | 20 | 3 | 345 | 8 | 403 | 1.86e-29 | 117 |
| MS.gene24837.t1 | MTR_3g104880 | 42.593 | 216 | 100 | 8 | 145 | 341 | 161 | 371 | 3.82e-23 | 99.4 |
| MS.gene24837.t1 | MTR_1g015275 | 33.056 | 360 | 193 | 10 | 15 | 335 | 17 | 367 | 5.51e-16 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24837.t1 | AT3G42800 | 35.083 | 362 | 188 | 12 | 7 | 345 | 2 | 339 | 5.23e-38 | 139 |
| MS.gene24837.t1 | AT3G13980 | 46.119 | 219 | 90 | 11 | 147 | 346 | 147 | 356 | 1.63e-35 | 132 |
| MS.gene24837.t1 | AT1G54200 | 37.673 | 361 | 175 | 12 | 13 | 342 | 24 | 365 | 1.68e-30 | 119 |
Find 61 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCTTATTGATTTCTAAAT+TGG | 0.180371 | 8.4:-9307631 | None:intergenic |
| GAATTGGATTCATATGTTTC+TGG | 0.232164 | 8.4:-9306984 | None:intergenic |
| ATGATCAACAAAATCGGTTT+TGG | 0.255049 | 8.4:-9306728 | None:intergenic |
| TTTCTGGGGTGGATTGTATT+TGG | 0.276683 | 8.4:-9306968 | None:intergenic |
| CACATTGTGAGAATTGAAAA+TGG | 0.279236 | 8.4:-9307178 | None:intergenic |
| TTGAGAGCTTTGAAAATCTA+TGG | 0.303700 | 8.4:+9307090 | MS.gene24837:CDS |
| AGAAGAAGAAGCAACAAGTT+TGG | 0.318823 | 8.4:+9307043 | MS.gene24837:CDS |
| TCTGAATTGCTATGATGAAT+TGG | 0.319780 | 8.4:-9307000 | None:intergenic |
| GAATATTAACACGGTTGTTA+TGG | 0.321951 | 8.4:+9307464 | MS.gene24837:CDS |
| ATCAATAAGGCCATTGCTAA+TGG | 0.341844 | 8.4:+9307642 | MS.gene24837:CDS |
| TCACCTAGGATTACGCTAAC+AGG | 0.349813 | 8.4:-9307369 | None:intergenic |
| GAAGAAGAAGCAACAAGTTT+GGG | 0.350251 | 8.4:+9307044 | MS.gene24837:CDS |
| TTTGAGCTTGATCATCTTGT+TGG | 0.353919 | 8.4:+9307567 | MS.gene24837:CDS |
| AAATTGGTAGTTTCATAAAC+TGG | 0.360593 | 8.4:-9307615 | None:intergenic |
| TAGTCTACTCCCTGGTGAAA+TGG | 0.366874 | 8.4:-9307139 | None:intergenic |
| GAAGGAGTTCTTCTCCTTTG+TGG | 0.369470 | 8.4:-9306658 | None:intergenic |
| AAGCAACAAGTTTGGGAAGA+TGG | 0.369581 | 8.4:+9307051 | MS.gene24837:CDS |
| TGCTTTGAGTTGTTCGAGTT+CGG | 0.377587 | 8.4:+9307539 | MS.gene24837:CDS |
| TGTTGGAGGTGGAAGGTATC+AGG | 0.383104 | 8.4:+9307584 | MS.gene24837:CDS |
| TACCAATTTAGAAATCAATA+AGG | 0.406180 | 8.4:+9307629 | MS.gene24837:CDS |
| AATATTCTTCAGCTCATTAA+CGG | 0.409083 | 8.4:-9307448 | None:intergenic |
| AATTGGATTCATATGTTTCT+GGG | 0.415683 | 8.4:-9306983 | None:intergenic |
| ACGAAGATTCATTCTCTGCT+TGG | 0.428357 | 8.4:-9306821 | None:intergenic |
| CGGTTTCTTCATATTCTAGA+AGG | 0.430753 | 8.4:+9307283 | MS.gene24837:CDS |
| GTAATCTTTCGAACAGTACT+AGG | 0.436399 | 8.4:-9307414 | None:intergenic |
| GTTTACATGATCAACAAAAT+CGG | 0.436424 | 8.4:-9306734 | None:intergenic |
| AGTCTACTCCCTGGTGAAAT+GGG | 0.441149 | 8.4:-9307138 | None:intergenic |
| ATCAATGGTGTCAAGAAGAA+TGG | 0.470483 | 8.4:-9306689 | None:intergenic |
| GAGCTTGATCATCTTGTTGG+AGG | 0.472372 | 8.4:+9307570 | MS.gene24837:CDS |
| AGCTCCTCAGAATGTAGCTC+TGG | 0.476023 | 8.4:+9306889 | MS.gene24837:CDS |
| CGGTTGTTATGGCTAAAGAA+AGG | 0.496287 | 8.4:+9307475 | MS.gene24837:CDS |
| AGAAGAATGGAGGAGAAAGA+AGG | 0.505994 | 8.4:-9306676 | None:intergenic |
| AAGCCGTGATGATAGAAGAT+TGG | 0.513145 | 8.4:+9306845 | MS.gene24837:CDS |
| CTTGATCATCTTGTTGGAGG+TGG | 0.520355 | 8.4:+9307573 | MS.gene24837:CDS |
| AAACAATGGTGTTAAAAGAT+CGG | 0.530728 | 8.4:+9307335 | MS.gene24837:CDS |
| ATCATCTTGTTGGAGGTGGA+AGG | 0.534714 | 8.4:+9307577 | MS.gene24837:CDS |
| GTGAAGCAACCCATTTCACC+AGG | 0.553164 | 8.4:+9307129 | MS.gene24837:CDS |
| AGAGAAACAACAGAAGAAGA+AGG | 0.564963 | 8.4:+9307020 | MS.gene24837:CDS |
| GGATTCATATGTTTCTGGGG+TGG | 0.565291 | 8.4:-9306979 | None:intergenic |
| CGTGATGATAGAAGATTGGA+TGG | 0.581024 | 8.4:+9306849 | MS.gene24837:CDS |
| AATGTAGCTAGTCTACTCCC+TGG | 0.582884 | 8.4:-9307147 | None:intergenic |
| TCCTCAGAATGTAGCTCTGG+TGG | 0.587097 | 8.4:+9306892 | MS.gene24837:CDS |
| ATAGGTTGATTGTAGAGACA+TGG | 0.588880 | 8.4:-9306763 | None:intergenic |
| TTCGTCGATAGAATGATCAA+TGG | 0.597673 | 8.4:-9306704 | None:intergenic |
| TACCCTGTTAGCGTAATCCT+AGG | 0.598810 | 8.4:+9307366 | MS.gene24837:CDS |
| AAAGCTAAAATGTGTTACGT+TGG | 0.599710 | 8.4:+9307204 | MS.gene24837:CDS |
| CATCCAATCTTCTATCATCA+CGG | 0.601460 | 8.4:-9306848 | None:intergenic |
| TCCACCAGAGCTACATTCTG+AGG | 0.607594 | 8.4:-9306893 | None:intergenic |
| GAGAGAGATCTTATCCACAA+AGG | 0.608634 | 8.4:+9306644 | MS.gene24837:CDS |
| CACCTAGGATTACGCTAACA+GGG | 0.609113 | 8.4:-9307368 | None:intergenic |
| GTTGTTCAGAATCCTCACCT+AGG | 0.616647 | 8.4:-9307383 | None:intergenic |
| TTAACACAAACCATTAGCAA+TGG | 0.617526 | 8.4:-9307652 | None:intergenic |
| GTAAGATGAATCTGTTTCTG+AGG | 0.625400 | 8.4:-9306926 | None:intergenic |
| AATGGTGTCAAGAAGAATGG+AGG | 0.631008 | 8.4:-9306686 | None:intergenic |
| GTTGATTGTAGAGACATGGT+TGG | 0.635301 | 8.4:-9306759 | None:intergenic |
| ATTGGATTCATATGTTTCTG+GGG | 0.644164 | 8.4:-9306982 | None:intergenic |
| TGTTAGCGTAATCCTAGGTG+AGG | 0.668825 | 8.4:+9307371 | MS.gene24837:CDS |
| ACACCTTCAAAAGCAAACAA+TGG | 0.669992 | 8.4:+9307321 | MS.gene24837:CDS |
| TCTAGAATATGAAGAAACCG+AGG | 0.699436 | 8.4:-9307280 | None:intergenic |
| TGAGCTGAAGAATATTAACA+CGG | 0.710175 | 8.4:+9307455 | MS.gene24837:CDS |
| TGAAGCAACCCATTTCACCA+GGG | 0.737178 | 8.4:+9307130 | MS.gene24837:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACCAATTTAGAAATCAATA+AGG | + | chr8.4:9307629-9307648 | MS.gene24837:CDS | 20.0% |
| !!! | AAAGAAAGGTTTTATGATAA+TGG | + | chr8.4:9307489-9307508 | MS.gene24837:CDS | 20.0% |
| ! | AATATTCTTCAGCTCATTAA+CGG | - | chr8.4:9307451-9307470 | None:intergenic | 25.0% |
| ! | AATTGGATTCATATGTTTCT+GGG | - | chr8.4:9306986-9307005 | None:intergenic | 25.0% |
| ! | GTTTACATGATCAACAAAAT+CGG | - | chr8.4:9306737-9306756 | None:intergenic | 25.0% |
| !! | AAACAATGGTGTTAAAAGAT+CGG | + | chr8.4:9307335-9307354 | MS.gene24837:CDS | 25.0% |
| !! | AAATTGGTAGTTTCATAAAC+TGG | - | chr8.4:9307618-9307637 | None:intergenic | 25.0% |
| !! | TTGAATTTGACGTGTTTTAT+AGG | - | chr8.4:9306784-9306803 | None:intergenic | 25.0% |
| AAAGCTAAAATGTGTTACGT+TGG | + | chr8.4:9307204-9307223 | MS.gene24837:CDS | 30.0% | |
| CACATTGTGAGAATTGAAAA+TGG | - | chr8.4:9307181-9307200 | None:intergenic | 30.0% | |
| GAATATTAACACGGTTGTTA+TGG | + | chr8.4:9307464-9307483 | MS.gene24837:CDS | 30.0% | |
| GAATTGGATTCATATGTTTC+TGG | - | chr8.4:9306987-9307006 | None:intergenic | 30.0% | |
| TCTGAATTGCTATGATGAAT+TGG | - | chr8.4:9307003-9307022 | None:intergenic | 30.0% | |
| TGAGCTGAAGAATATTAACA+CGG | + | chr8.4:9307455-9307474 | MS.gene24837:CDS | 30.0% | |
| TTAACACAAACCATTAGCAA+TGG | - | chr8.4:9307655-9307674 | None:intergenic | 30.0% | |
| ! | ATTGGATTCATATGTTTCTG+GGG | - | chr8.4:9306985-9307004 | None:intergenic | 30.0% |
| !! | ATGATCAACAAAATCGGTTT+TGG | - | chr8.4:9306731-9306750 | None:intergenic | 30.0% |
| !! | GGCCTTATTGATTTCTAAAT+TGG | - | chr8.4:9307634-9307653 | None:intergenic | 30.0% |
| !! | TTGAGAGCTTTGAAAATCTA+TGG | + | chr8.4:9307090-9307109 | MS.gene24837:CDS | 30.0% |
| ACACCTTCAAAAGCAAACAA+TGG | + | chr8.4:9307321-9307340 | MS.gene24837:CDS | 35.0% | |
| AGAAGAAGAAGCAACAAGTT+TGG | + | chr8.4:9307043-9307062 | MS.gene24837:CDS | 35.0% | |
| AGAGAAACAACAGAAGAAGA+AGG | + | chr8.4:9307020-9307039 | MS.gene24837:CDS | 35.0% | |
| ATCAATAAGGCCATTGCTAA+TGG | + | chr8.4:9307642-9307661 | MS.gene24837:CDS | 35.0% | |
| CATCCAATCTTCTATCATCA+CGG | - | chr8.4:9306851-9306870 | None:intergenic | 35.0% | |
| CGGTTTCTTCATATTCTAGA+AGG | + | chr8.4:9307283-9307302 | MS.gene24837:CDS | 35.0% | |
| GAAGAAGAAGCAACAAGTTT+GGG | + | chr8.4:9307044-9307063 | MS.gene24837:CDS | 35.0% | |
| GTAATCTTTCGAACAGTACT+AGG | - | chr8.4:9307417-9307436 | None:intergenic | 35.0% | |
| TTCGTCGATAGAATGATCAA+TGG | - | chr8.4:9306707-9306726 | None:intergenic | 35.0% | |
| TTTGAGCTTGATCATCTTGT+TGG | + | chr8.4:9307567-9307586 | MS.gene24837:CDS | 35.0% | |
| ! | ATCAATGGTGTCAAGAAGAA+TGG | - | chr8.4:9306692-9306711 | None:intergenic | 35.0% |
| ! | TCTAGAATATGAAGAAACCG+AGG | - | chr8.4:9307283-9307302 | None:intergenic | 35.0% |
| !! | ACACCATTGTTTGCTTTTGA+AGG | - | chr8.4:9307327-9307346 | None:intergenic | 35.0% |
| !! | ATAGGTTGATTGTAGAGACA+TGG | - | chr8.4:9306766-9306785 | None:intergenic | 35.0% |
| !! | GTAAGATGAATCTGTTTCTG+AGG | - | chr8.4:9306929-9306948 | None:intergenic | 35.0% |
| AAGCAACAAGTTTGGGAAGA+TGG | + | chr8.4:9307051-9307070 | MS.gene24837:CDS | 40.0% | |
| AAGCCGTGATGATAGAAGAT+TGG | + | chr8.4:9306845-9306864 | MS.gene24837:CDS | 40.0% | |
| ACGAAGATTCATTCTCTGCT+TGG | - | chr8.4:9306824-9306843 | None:intergenic | 40.0% | |
| AGAAGAATGGAGGAGAAAGA+AGG | - | chr8.4:9306679-9306698 | None:intergenic | 40.0% | |
| CGGTTGTTATGGCTAAAGAA+AGG | + | chr8.4:9307475-9307494 | MS.gene24837:CDS | 40.0% | |
| CGTGATGATAGAAGATTGGA+TGG | + | chr8.4:9306849-9306868 | MS.gene24837:CDS | 40.0% | |
| GAAACCGAGGAAGAAAAACA+AGG | - | chr8.4:9307270-9307289 | None:intergenic | 40.0% | |
| GAGAGAGATCTTATCCACAA+AGG | + | chr8.4:9306644-9306663 | MS.gene24837:CDS | 40.0% | |
| GTTGATTGTAGAGACATGGT+TGG | - | chr8.4:9306762-9306781 | None:intergenic | 40.0% | |
| ! | AATGGTGTCAAGAAGAATGG+AGG | - | chr8.4:9306689-9306708 | None:intergenic | 40.0% |
| !! | TGCTTTGAGTTGTTCGAGTT+CGG | + | chr8.4:9307539-9307558 | MS.gene24837:CDS | 40.0% |
| !! | TTTCTGGGGTGGATTGTATT+TGG | - | chr8.4:9306971-9306990 | None:intergenic | 40.0% |
| !!! | GTCACCTTGTTTTTCTTCCT+CGG | + | chr8.4:9307263-9307282 | MS.gene24837:CDS | 40.0% |
| AGTCTACTCCCTGGTGAAAT+GGG | - | chr8.4:9307141-9307160 | None:intergenic | 45.0% | |
| ATCATCTTGTTGGAGGTGGA+AGG | + | chr8.4:9307577-9307596 | MS.gene24837:CDS | 45.0% | |
| CACCTAGGATTACGCTAACA+GGG | - | chr8.4:9307371-9307390 | None:intergenic | 45.0% | |
| CTTGATCATCTTGTTGGAGG+TGG | + | chr8.4:9307573-9307592 | MS.gene24837:CDS | 45.0% | |
| GAAGGAGTTCTTCTCCTTTG+TGG | - | chr8.4:9306661-9306680 | None:intergenic | 45.0% | |
| GAGCTTGATCATCTTGTTGG+AGG | + | chr8.4:9307570-9307589 | MS.gene24837:CDS | 45.0% | |
| GTTGTTCAGAATCCTCACCT+AGG | - | chr8.4:9307386-9307405 | None:intergenic | 45.0% | |
| TACCCTGTTAGCGTAATCCT+AGG | + | chr8.4:9307366-9307385 | MS.gene24837:CDS | 45.0% | |
| TAGTCTACTCCCTGGTGAAA+TGG | - | chr8.4:9307142-9307161 | None:intergenic | 45.0% | |
| TCACCTAGGATTACGCTAAC+AGG | - | chr8.4:9307372-9307391 | None:intergenic | 45.0% | |
| TGAAGCAACCCATTTCACCA+GGG | + | chr8.4:9307130-9307149 | MS.gene24837:CDS | 45.0% | |
| TGTTAGCGTAATCCTAGGTG+AGG | + | chr8.4:9307371-9307390 | MS.gene24837:CDS | 45.0% | |
| ! | AATGTAGCTAGTCTACTCCC+TGG | - | chr8.4:9307150-9307169 | None:intergenic | 45.0% |
| !! | GGATTCATATGTTTCTGGGG+TGG | - | chr8.4:9306982-9307001 | None:intergenic | 45.0% |
| AGCTCCTCAGAATGTAGCTC+TGG | + | chr8.4:9306889-9306908 | MS.gene24837:CDS | 50.0% | |
| GTGAAGCAACCCATTTCACC+AGG | + | chr8.4:9307129-9307148 | MS.gene24837:CDS | 50.0% | |
| TCCACCAGAGCTACATTCTG+AGG | - | chr8.4:9306896-9306915 | None:intergenic | 50.0% | |
| !! | TCCTCAGAATGTAGCTCTGG+TGG | + | chr8.4:9306892-9306911 | MS.gene24837:CDS | 50.0% |
| !! | TGTTGGAGGTGGAAGGTATC+AGG | + | chr8.4:9307584-9307603 | MS.gene24837:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9306634 | 9307674 | 9306634 | ID=MS.gene24837 |
| chr8.4 | mRNA | 9306634 | 9307674 | 9306634 | ID=MS.gene24837.t1;Parent=MS.gene24837 |
| chr8.4 | exon | 9306634 | 9307674 | 9306634 | ID=MS.gene24837.t1.exon1;Parent=MS.gene24837.t1 |
| chr8.4 | CDS | 9306634 | 9307674 | 9306634 | ID=cds.MS.gene24837.t1;Parent=MS.gene24837.t1 |
| Gene Sequence |
| Protein sequence |
