Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24988.t1 | RHN68985.1 | 67 | 94 | 15 | 2 | 1 | 92 | 1 | 80 | 2.10E-24 | 122.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24988.t1 | A0A396IY39 | 67.0 | 94 | 15 | 2 | 1 | 92 | 1 | 80 | 1.5e-24 | 122.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052813 | MS.gene24988 | 0.81889 | 1.55E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24988.t1 | MTR_4g108770 | 64.894 | 94 | 22 | 2 | 1 | 92 | 1 | 85 | 2.86e-28 | 100 |
| MS.gene24988.t1 | MTR_7g099240 | 76.667 | 60 | 12 | 2 | 36 | 93 | 65 | 124 | 1.43e-24 | 95.1 |
| MS.gene24988.t1 | MTR_3g062480 | 53.125 | 64 | 16 | 1 | 1 | 64 | 1 | 50 | 3.70e-15 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGGATGATAGCTTGAAAT+TGG | 0.231205 | 4.1:+82205440 | None:intergenic |
| CGTACCTCATCTTGGATGTT+AGG | 0.257541 | 4.1:+82205422 | None:intergenic |
| CCTCCTCATCACGACTTCTT+TGG | 0.267013 | 4.1:+82204434 | None:intergenic |
| GTACGTAAAGCTTACCTTAA+AGG | 0.274677 | 4.1:-82205404 | MS.gene24988:CDS |
| GGGATGGATCATTGCTCTCT+TGG | 0.301522 | 4.1:-82204494 | MS.gene24988:CDS |
| TAGGTTTATAGCGACCTTTA+AGG | 0.343333 | 4.1:+82205390 | None:intergenic |
| AGTTGTTGATTACAATTTGT+TGG | 0.345936 | 4.1:-82205486 | MS.gene24988:CDS |
| GGATGGATCATTGCTCTCTT+GGG | 0.360508 | 4.1:-82204493 | MS.gene24988:CDS |
| CTTGGATGTCGAGTATTTGA+TGG | 0.385550 | 4.1:+82205557 | None:intergenic |
| TTTAGTGATGATGAACCGTT+TGG | 0.389714 | 4.1:-82204515 | MS.gene24988:CDS |
| AAGCTTTACGTACCTCATCT+TGG | 0.412556 | 4.1:+82205414 | None:intergenic |
| ATACCCTCATTGGCCGAGTT+AGG | 0.430938 | 4.1:+82205512 | None:intergenic |
| TAGAGACAAAGCTAGTTCTT+CGG | 0.451946 | 4.1:-82205591 | MS.gene24988:CDS |
| TGATTACAATTTGTTGGAAA+CGG | 0.453503 | 4.1:-82205480 | MS.gene24988:CDS |
| AATTTGTTGGAAACGGATCC+AGG | 0.463546 | 4.1:-82205473 | MS.gene24988:CDS |
| GTTTACCCTTGGTCTGATCA+TGG | 0.465118 | 4.1:-82205359 | MS.gene24988:intron |
| CTAGCAATTTCGTTTACCCT+TGG | 0.471488 | 4.1:-82205370 | MS.gene24988:CDS |
| TTTATTATCACCTCATCCTC+AGG | 0.489201 | 4.1:+82204403 | None:intergenic |
| GAAATTGGAGACCTAAGTCC+TGG | 0.495267 | 4.1:+82205455 | None:intergenic |
| GGGTACTACTCCTGAGGATG+AGG | 0.496888 | 4.1:-82204413 | MS.gene24988:CDS |
| TGGAAACGGATCCAGGACTT+AGG | 0.504481 | 4.1:-82205466 | MS.gene24988:CDS |
| AACAACTTGAATACCCTCAT+TGG | 0.512185 | 4.1:+82205502 | None:intergenic |
| CAAGGGTAAACGAAATTGCT+AGG | 0.534788 | 4.1:+82205371 | None:intergenic |
| AGCTTTGTCTCTAGAAAGCA+CGG | 0.558118 | 4.1:+82205601 | None:intergenic |
| TTAGTGATGATGAACCGTTT+GGG | 0.566769 | 4.1:-82204514 | MS.gene24988:CDS |
| CCAAAGAAGTCGTGATGAGG+AGG | 0.574893 | 4.1:-82204434 | MS.gene24988:CDS |
| CATCACGACTTCTTTGGACA+CGG | 0.575920 | 4.1:+82204440 | None:intergenic |
| GAGCAATGATCCATCCCAAA+CGG | 0.582044 | 4.1:+82204500 | None:intergenic |
| TACACCTAACTCGGCCAATG+AGG | 0.584724 | 4.1:-82205516 | MS.gene24988:CDS |
| TGATGATGAACCGTTTGGGA+TGG | 0.586248 | 4.1:-82204510 | MS.gene24988:CDS |
| AAATGAAGTTACACCTAACT+CGG | 0.604714 | 4.1:-82205525 | MS.gene24988:CDS |
| ACACCTAACTCGGCCAATGA+GGG | 0.613513 | 4.1:-82205515 | MS.gene24988:CDS |
| GTCTACCATGATCAGACCAA+GGG | 0.631510 | 4.1:+82205354 | None:intergenic |
| TGTCCAAAGAAGTCGTGATG+AGG | 0.632393 | 4.1:-82204437 | MS.gene24988:CDS |
| CAAAGAAGTCGTGATGAGGA+GGG | 0.645753 | 4.1:-82204433 | MS.gene24988:CDS |
| TCATCCTAACATCCAAGATG+AGG | 0.653385 | 4.1:-82205426 | MS.gene24988:CDS |
| TGTCTACCATGATCAGACCA+AGG | 0.681573 | 4.1:+82205353 | None:intergenic |
| GCTTTGTCTCTAGAAAGCAC+GGG | 0.696977 | 4.1:+82205602 | None:intergenic |
| TGAGGAGGGTACTACTCCTG+AGG | 0.705059 | 4.1:-82204419 | MS.gene24988:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTATCATTATCATTTTAA+AGG | - | chr4.1:82205062-82205081 | MS.gene24988:intron | 15.0% |
| !!! | ATTCATTAGTTTTAAAAGAT+TGG | + | chr4.1:82205447-82205466 | None:intergenic | 15.0% |
| !!! | ATTGGTTTAATTTGTATGAT+TGG | - | chr4.1:82204703-82204722 | MS.gene24988:intron | 20.0% |
| !!! | GATAACTTCTTTTTCAATAA+TGG | + | chr4.1:82205049-82205068 | None:intergenic | 20.0% |
| !!! | TCTATTTGTCTTTTATAAAG+AGG | - | chr4.1:82204890-82204909 | MS.gene24988:intron | 20.0% |
| ! | GATAATTCAAACATGTATAC+TGG | - | chr4.1:82205114-82205133 | MS.gene24988:intron | 25.0% |
| ! | TAAAGGAATATGTGATGATA+TGG | - | chr4.1:82205079-82205098 | MS.gene24988:intron | 25.0% |
| ! | TTTCAACTTTATCTCGTATA+TGG | + | chr4.1:82205256-82205275 | None:intergenic | 25.0% |
| !! | AGTTGTTGATTACAATTTGT+TGG | - | chr4.1:82204527-82204546 | MS.gene24988:CDS | 25.0% |
| !!! | AACTTCTTTTTCAATAATGG+TGG | + | chr4.1:82205046-82205065 | None:intergenic | 25.0% |
| !!! | AAGTTTTAGACTTATTAGAC+TGG | - | chr4.1:82204992-82205011 | MS.gene24988:intron | 25.0% |
| !!! | TGATTACAATTTGTTGGAAA+CGG | - | chr4.1:82204533-82204552 | MS.gene24988:intron | 25.0% |
| AAATGAAGTTACACCTAACT+CGG | - | chr4.1:82204488-82204507 | MS.gene24988:CDS | 30.0% | |
| ATTCAAACATGTATACTGGT+TGG | - | chr4.1:82205118-82205137 | MS.gene24988:intron | 30.0% | |
| GATTGAGTATAGTGAATCTA+AGG | - | chr4.1:82204738-82204757 | MS.gene24988:intron | 30.0% | |
| GTATGATTGGATTGTATGAT+TGG | - | chr4.1:82204716-82204735 | MS.gene24988:intron | 30.0% | |
| TAGTATTTGTATACGAGTTC+AGG | - | chr4.1:82205195-82205214 | MS.gene24988:intron | 30.0% | |
| TTAGGATGATAGCTTGAAAT+TGG | + | chr4.1:82204576-82204595 | None:intergenic | 30.0% | |
| ! | GACGTAGATTTTGTAAGAAT+TGG | - | chr4.1:82204685-82204704 | MS.gene24988:intron | 30.0% |
| ! | TCTTGCAAACAAAGCATTTA+AGG | - | chr4.1:82205282-82205301 | MS.gene24988:intron | 30.0% |
| ! | TTAGGTGTAACTTCATTTTG+TGG | + | chr4.1:82204486-82204505 | None:intergenic | 30.0% |
| AACAACTTGAATACCCTCAT+TGG | + | chr4.1:82204514-82204533 | None:intergenic | 35.0% | |
| AGTTTCTCAGAAAGATGAGA+AGG | - | chr4.1:82204969-82204988 | MS.gene24988:intron | 35.0% | |
| ATCCAGACTTTCATTATACG+TGG | + | chr4.1:82204781-82204800 | None:intergenic | 35.0% | |
| CGGTTCATCATCACTAAAAA+TGG | + | chr4.1:82205496-82205515 | None:intergenic | 35.0% | |
| GTACGTAAAGCTTACCTTAA+AGG | - | chr4.1:82204609-82204628 | MS.gene24988:intron | 35.0% | |
| TACGTGGAGCTAAAATTTCT+AGG | + | chr4.1:82204765-82204784 | None:intergenic | 35.0% | |
| TAGGTTTATAGCGACCTTTA+AGG | + | chr4.1:82204626-82204645 | None:intergenic | 35.0% | |
| TTTATTATCACCTCATCCTC+AGG | + | chr4.1:82205613-82205632 | None:intergenic | 35.0% | |
| TTTCTTTCCAAACTCAGTCT+CGG | - | chr4.1:82205382-82205401 | MS.gene24988:CDS | 35.0% | |
| ! | ACTTCATTTTGTGGTTCACT+TGG | + | chr4.1:82204477-82204496 | None:intergenic | 35.0% |
| ! | AGAGAGTAGTATTGAATGAG+TGG | - | chr4.1:82204918-82204937 | MS.gene24988:intron | 35.0% |
| ! | CTGAATTTTCTATAACCGTG+TGG | + | chr4.1:82204945-82204964 | None:intergenic | 35.0% |
| ! | TAGAGACAAAGCTAGTTCTT+CGG | - | chr4.1:82204422-82204441 | MS.gene24988:CDS | 35.0% |
| ! | TTAGTGATGATGAACCGTTT+GGG | - | chr4.1:82205499-82205518 | MS.gene24988:CDS | 35.0% |
| ! | TTTAGTGATGATGAACCGTT+TGG | - | chr4.1:82205498-82205517 | MS.gene24988:CDS | 35.0% |
| !! | AGTTCAGGCTTTAAACTGTA+AGG | - | chr4.1:82205210-82205229 | MS.gene24988:intron | 35.0% |
| AAGCTTTACGTACCTCATCT+TGG | + | chr4.1:82204602-82204621 | None:intergenic | 40.0% | |
| AGTCCTATTCAACGCTTTGT+CGG | - | chr4.1:82205157-82205176 | MS.gene24988:intron | 40.0% | |
| ATGTAAACCGAGACTGAGTT+TGG | + | chr4.1:82205392-82205411 | None:intergenic | 40.0% | |
| ATTCAAACACCTCTCATCAG+GGG | + | chr4.1:82204807-82204826 | None:intergenic | 40.0% | |
| CAAGGGTAAACGAAATTGCT+AGG | + | chr4.1:82204645-82204664 | None:intergenic | 40.0% | |
| CATTCAAACACCTCTCATCA+GGG | + | chr4.1:82204808-82204827 | None:intergenic | 40.0% | |
| CTAGCAATTTCGTTTACCCT+TGG | - | chr4.1:82204643-82204662 | MS.gene24988:intron | 40.0% | |
| CTCCACGTATAATGAAAGTC+TGG | - | chr4.1:82204776-82204795 | MS.gene24988:intron | 40.0% | |
| CTTGGATGTCGAGTATTTGA+TGG | + | chr4.1:82204459-82204478 | None:intergenic | 40.0% | |
| TATTGAATGAGTGGTCCACA+CGG | - | chr4.1:82204927-82204946 | MS.gene24988:intron | 40.0% | |
| TCAGTCTCGGTTTACATTGA+GGG | - | chr4.1:82205395-82205414 | MS.gene24988:CDS | 40.0% | |
| TCATCCTAACATCCAAGATG+AGG | - | chr4.1:82204587-82204606 | MS.gene24988:intron | 40.0% | |
| TCATTCAAACACCTCTCATC+AGG | + | chr4.1:82204809-82204828 | None:intergenic | 40.0% | |
| ! | AGCTTTGTCTCTAGAAAGCA+CGG | + | chr4.1:82204415-82204434 | None:intergenic | 40.0% |
| !! | AATTTGTTGGAAACGGATCC+AGG | - | chr4.1:82204540-82204559 | MS.gene24988:intron | 40.0% |
| !! | ACAAAGCGTTGAATAGGACT+GGG | + | chr4.1:82205157-82205176 | None:intergenic | 40.0% |
| CAAAGAAGTCGTGATGAGGA+GGG | - | chr4.1:82205580-82205599 | MS.gene24988:CDS | 45.0% | |
| CATCACGACTTCTTTGGACA+CGG | + | chr4.1:82205576-82205595 | None:intergenic | 45.0% | |
| CGTACCTCATCTTGGATGTT+AGG | + | chr4.1:82204594-82204613 | None:intergenic | 45.0% | |
| CTCAGTCTCGGTTTACATTG+AGG | - | chr4.1:82205394-82205413 | MS.gene24988:CDS | 45.0% | |
| GAAATTGGAGACCTAAGTCC+TGG | + | chr4.1:82204561-82204580 | None:intergenic | 45.0% | |
| GAGCAATGATCCATCCCAAA+CGG | + | chr4.1:82205516-82205535 | None:intergenic | 45.0% | |
| GGATGGATCATTGCTCTCTT+GGG | - | chr4.1:82205520-82205539 | MS.gene24988:CDS | 45.0% | |
| GTCTACCATGATCAGACCAA+GGG | + | chr4.1:82204662-82204681 | None:intergenic | 45.0% | |
| GTTTACCCTTGGTCTGATCA+TGG | - | chr4.1:82204654-82204673 | MS.gene24988:intron | 45.0% | |
| TGTCCAAAGAAGTCGTGATG+AGG | - | chr4.1:82205576-82205595 | MS.gene24988:CDS | 45.0% | |
| TGTCTACCATGATCAGACCA+AGG | + | chr4.1:82204663-82204682 | None:intergenic | 45.0% | |
| ! | GCTTTGTCTCTAGAAAGCAC+GGG | + | chr4.1:82204414-82204433 | None:intergenic | 45.0% |
| ! | TGATGATGAACCGTTTGGGA+TGG | - | chr4.1:82205503-82205522 | MS.gene24988:CDS | 45.0% |
| !! | ACTCCGACAAAGCGTTGAAT+AGG | + | chr4.1:82205163-82205182 | None:intergenic | 45.0% |
| !! | GACAAAGCGTTGAATAGGAC+TGG | + | chr4.1:82205158-82205177 | None:intergenic | 45.0% |
| ACACCTAACTCGGCCAATGA+GGG | - | chr4.1:82204498-82204517 | MS.gene24988:CDS | 50.0% | |
| ATACCCTCATTGGCCGAGTT+AGG | + | chr4.1:82204504-82204523 | None:intergenic | 50.0% | |
| CCAAAGAAGTCGTGATGAGG+AGG | - | chr4.1:82205579-82205598 | MS.gene24988:CDS | 50.0% | |
| CCTCCTCATCACGACTTCTT+TGG | + | chr4.1:82205582-82205601 | None:intergenic | 50.0% | |
| GGGATGGATCATTGCTCTCT+TGG | - | chr4.1:82205519-82205538 | MS.gene24988:CDS | 50.0% | |
| TACACCTAACTCGGCCAATG+AGG | - | chr4.1:82204497-82204516 | MS.gene24988:CDS | 50.0% | |
| TGGAAACGGATCCAGGACTT+AGG | - | chr4.1:82204547-82204566 | MS.gene24988:intron | 50.0% | |
| CAAACACCTCTCATCAGGGG+CGG | + | chr4.1:82204804-82204823 | None:intergenic | 55.0% | |
| GGGTACTACTCCTGAGGATG+AGG | - | chr4.1:82205600-82205619 | MS.gene24988:CDS | 55.0% | |
| !! | TGAGGAGGGTACTACTCCTG+AGG | - | chr4.1:82205594-82205613 | MS.gene24988:CDS | 55.0% |
| CTGGATCCGCCCCTGATGAG+AGG | - | chr4.1:82204795-82204814 | MS.gene24988:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 82204396 | 82205639 | 82204396 | ID=MS.gene24988 |
| chr4.1 | mRNA | 82204396 | 82205639 | 82204396 | ID=MS.gene24988.t1;Parent=MS.gene24988 |
| chr4.1 | exon | 82205360 | 82205639 | 82205360 | ID=MS.gene24988.t1.exon1;Parent=MS.gene24988.t1 |
| chr4.1 | CDS | 82205360 | 82205639 | 82205360 | ID=cds.MS.gene24988.t1;Parent=MS.gene24988.t1 |
| chr4.1 | exon | 82204396 | 82204547 | 82204396 | ID=MS.gene24988.t1.exon2;Parent=MS.gene24988.t1 |
| chr4.1 | CDS | 82204396 | 82204547 | 82204396 | ID=cds.MS.gene24988.t1;Parent=MS.gene24988.t1 |
| Gene Sequence |
| Protein sequence |
