AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25034

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25034.t1 MTR_8g023400 95.268 317 15 0 3 319 5 321 0.0 558
MS.gene25034.t1 MTR_7g117540 41.036 251 112 6 15 261 12 230 3.72e-44 153
MS.gene25034.t1 MTR_4g120270 42.697 178 75 7 19 178 13 181 2.13e-32 121
MS.gene25034.t1 MTR_2g436100 37.143 175 93 5 17 179 23 192 4.64e-31 118
MS.gene25034.t1 MTR_5g064890 30.288 208 138 5 27 229 63 268 2.32e-23 99.0
MS.gene25034.t1 MTR_3g464780 37.931 174 89 4 26 180 25 198 3.07e-23 96.7
MS.gene25034.t1 MTR_4g106680 35.385 195 92 8 27 201 48 228 3.18e-22 95.1
MS.gene25034.t1 MTR_5g034790 35.507 138 80 4 29 158 49 185 4.94e-22 95.5
MS.gene25034.t1 MTR_5g071930 39.860 143 68 3 27 163 7 137 3.50e-21 89.7
MS.gene25034.t1 MTR_8g063660 32.639 144 72 4 28 157 4 136 1.24e-20 90.9
MS.gene25034.t1 MTR_4g129420 38.793 116 62 3 44 157 78 186 1.62e-20 92.0
MS.gene25034.t1 MTR_5g024730 31.288 163 90 3 28 186 33 177 1.71e-20 88.2
MS.gene25034.t1 MTR_3g110190 52.778 72 33 1 90 160 66 137 6.20e-20 89.4
MS.gene25034.t1 MTR_6g087150 31.980 197 102 6 27 217 42 212 1.24e-19 88.6
MS.gene25034.t1 MTR_4g093830 32.584 178 107 4 30 194 58 235 1.47e-19 88.2
MS.gene25034.t1 MTR_8g092840 46.835 79 39 2 90 166 113 190 7.23e-19 84.3
MS.gene25034.t1 MTR_6g007737 36.957 138 71 5 29 157 71 201 7.32e-19 86.7
MS.gene25034.t1 MTR_8g092960 46.835 79 39 2 90 166 103 180 7.68e-19 84.0
MS.gene25034.t1 MTR_3g116000 44.737 76 42 0 90 165 79 154 9.63e-19 83.2
MS.gene25034.t1 MTR_1g074190 33.824 136 75 4 36 164 53 180 9.92e-19 85.9
MS.gene25034.t1 MTR_5g066250 44.186 86 47 1 77 161 121 206 1.13e-18 86.3
MS.gene25034.t1 MTR_1g100737 47.297 74 33 1 90 157 92 165 1.73e-18 82.4
MS.gene25034.t1 MTR_8g089880 36.792 106 56 2 89 193 126 221 1.92e-18 84.0
MS.gene25034.t1 MTR_1g043410 35.461 141 79 5 30 162 34 170 3.50e-18 84.0
MS.gene25034.t1 MTR_7g073200 43.158 95 51 2 87 179 78 171 3.70e-18 80.9
MS.gene25034.t1 MTR_8g093010 41.935 93 50 3 76 166 94 184 4.12e-18 82.0
MS.gene25034.t1 MTR_3g094000 47.222 72 35 1 90 158 91 162 4.38e-18 80.9
MS.gene25034.t1 MTR_2g090950 35.669 157 86 3 12 160 19 168 6.80e-18 81.6
MS.gene25034.t1 MTR_3g071300 30.573 157 90 5 17 168 17 159 7.28e-18 80.1
MS.gene25034.t1 MTR_7g113800 33.333 132 72 5 36 160 44 166 8.22e-18 83.2
MS.gene25034.t1 MTR_4g063070 35.252 139 69 6 33 157 247 378 8.59e-18 83.6
MS.gene25034.t1 MTR_3g100400 48.000 75 38 1 90 163 130 204 9.17e-18 83.6
MS.gene25034.t1 MTR_2g096350 42.308 104 48 3 60 160 86 180 1.01e-17 80.9
MS.gene25034.t1 MTR_8g092930 44.304 79 41 2 90 166 112 189 1.44e-17 80.5
MS.gene25034.t1 MTR_8g092985 44.304 79 41 2 90 166 111 188 1.64e-17 80.1
MS.gene25034.t1 MTR_3g086730 32.000 175 83 5 27 186 1 154 1.77e-17 80.5
MS.gene25034.t1 MTR_3g099650 35.915 142 68 7 26 160 57 182 2.43e-17 82.0
MS.gene25034.t1 MTR_8g073120 41.250 80 44 1 90 166 70 149 2.62e-17 79.3
MS.gene25034.t1 MTR_3g091620 40.230 87 46 2 79 164 95 176 3.20e-17 81.6
MS.gene25034.t1 MTR_1g090480 32.593 135 80 3 29 158 17 145 3.59e-17 78.2
MS.gene25034.t1 MTR_3g086700 34.783 138 62 4 27 157 1 117 4.02e-17 78.6
MS.gene25034.t1 MTR_5g064990 35.252 139 68 5 26 157 62 185 4.13e-17 81.6
MS.gene25034.t1 MTR_3g095560 46.154 78 39 2 90 165 109 185 4.40e-17 79.3
MS.gene25034.t1 MTR_5g030010 31.387 137 89 3 28 160 58 193 5.10e-17 81.3
MS.gene25034.t1 MTR_1g082550 47.368 76 35 2 90 160 98 173 6.83e-17 80.9
MS.gene25034.t1 MTR_6g055030 46.914 81 39 2 90 166 97 177 7.89e-17 80.1
MS.gene25034.t1 MTR_7g076250 41.463 82 38 2 78 157 59 132 1.02e-16 77.4
MS.gene25034.t1 MTR_2g009310 43.038 79 44 1 80 157 125 203 1.33e-16 80.1
MS.gene25034.t1 MTR_2g090905 44.156 77 36 1 91 160 40 116 1.68e-16 75.1
MS.gene25034.t1 MTR_7g108855 37.143 140 68 5 68 203 47 170 2.33e-16 76.6
MS.gene25034.t1 MTR_5g082940 31.858 113 62 2 54 163 51 151 3.35e-16 75.5
MS.gene25034.t1 MTR_4g030070 46.479 71 34 3 89 157 84 152 4.87e-16 76.3
MS.gene25034.t1 MTR_2g055790 47.059 68 34 1 90 157 77 142 2.40e-15 73.6
MS.gene25034.t1 MTR_8g092990 52.830 53 24 1 114 166 140 191 2.97e-15 74.3
MS.gene25034.t1 MTR_8g092970 52.830 53 24 1 114 166 130 181 3.11e-15 73.9
MS.gene25034.t1 MTR_1g070140 41.892 74 41 1 90 161 103 176 3.62e-15 75.5
MS.gene25034.t1 MTR_4g094725 42.105 76 41 1 90 162 78 153 1.02e-14 70.9
MS.gene25034.t1 MTR_1g492830 29.412 136 85 3 24 157 4 130 1.51e-14 72.0
MS.gene25034.t1 MTR_3g012550 38.961 77 44 1 85 158 75 151 1.87e-14 70.9
MS.gene25034.t1 MTR_5g026730 30.405 148 83 4 16 157 3 136 2.17e-14 70.5
MS.gene25034.t1 MTR_6g007680 28.859 149 80 3 29 176 4 127 2.46e-14 69.7
MS.gene25034.t1 MTR_3g114500 45.333 75 39 1 90 164 48 120 3.24e-14 68.9
MS.gene25034.t1 MTR_5g064980 43.056 72 37 1 90 157 108 179 3.67e-14 72.8
MS.gene25034.t1 MTR_8g066780 46.667 75 37 2 89 160 113 187 4.99e-14 72.4
MS.gene25034.t1 MTR_8g066740 30.822 146 87 4 90 228 76 214 7.83e-14 71.2
MS.gene25034.t1 MTR_1g026070 40.244 82 47 1 90 171 48 127 8.55e-14 67.8
MS.gene25034.t1 MTR_7g116220 38.667 75 38 2 90 158 70 142 8.60e-14 68.9
MS.gene25034.t1 MTR_2g035800 42.466 73 39 2 90 160 76 147 1.23e-13 68.2
MS.gene25034.t1 MTR_5g026720 56.250 48 21 0 111 158 89 136 1.51e-13 67.8
MS.gene25034.t1 MTR_4g056590 41.096 73 41 2 90 162 100 170 2.15e-13 68.2
MS.gene25034.t1 MTR_7g096690 29.452 146 78 7 27 161 15 146 1.23e-12 67.8
MS.gene25034.t1 MTR_5g076990 29.221 154 87 6 27 175 27 163 1.77e-12 67.4
MS.gene25034.t1 MTR_7g079280 35.616 73 44 1 89 158 79 151 2.07e-12 65.1
MS.gene25034.t1 MTR_8g071815 46.875 64 30 1 115 174 90 153 2.44e-12 65.1
MS.gene25034.t1 MTR_7g116180 38.356 73 41 2 90 158 67 139 2.47e-12 64.7
MS.gene25034.t1 MTR_1g090460 44.776 67 32 1 98 159 73 139 2.71e-12 64.3
MS.gene25034.t1 MTR_3g078450 36.000 75 45 1 87 158 73 147 2.95e-12 65.5
MS.gene25034.t1 MTR_1g070425 38.356 73 41 2 90 158 68 140 3.90e-12 64.3
MS.gene25034.t1 MTR_8g028465 28.261 138 93 3 25 158 22 157 3.91e-12 64.7
MS.gene25034.t1 MTR_7g116200 36.765 68 43 0 90 157 68 135 4.70e-12 63.9
MS.gene25034.t1 MTR_5g022920 57.778 45 19 0 113 157 187 231 5.13e-12 65.1
MS.gene25034.t1 MTR_8g099495 51.111 45 22 0 115 159 347 391 5.33e-12 66.6
MS.gene25034.t1 MTR_2g041200 59.574 47 19 0 114 160 108 154 5.40e-12 63.9
MS.gene25034.t1 MTR_7g079290 35.897 78 41 2 90 160 125 200 5.69e-12 64.7
MS.gene25034.t1 MTR_6g055030 56.000 50 22 0 117 166 97 146 8.08e-12 65.1
MS.gene25034.t1 MTR_7g059360 45.902 61 31 1 103 161 144 204 8.13e-12 64.3
MS.gene25034.t1 MTR_6g007670 39.130 69 42 0 96 164 14 82 9.33e-12 64.7
MS.gene25034.t1 MTR_8g072020 33.333 99 55 4 79 167 400 497 1.22e-11 65.5
MS.gene25034.t1 MTR_3g078470 36.000 75 45 2 87 158 73 147 1.51e-11 63.5
MS.gene25034.t1 MTR_8g072020 33.333 99 55 4 79 167 361 458 1.51e-11 65.1
MS.gene25034.t1 MTR_3g081030 44.444 72 36 3 88 157 92 161 1.55e-11 63.2
MS.gene25034.t1 MTR_8g032690 53.488 43 20 0 115 157 19 61 1.61e-11 60.8
MS.gene25034.t1 MTR_4g108830 43.056 72 39 2 90 160 121 191 1.68e-11 62.8
MS.gene25034.t1 MTR_4g108830 42.857 70 38 2 90 158 149 217 1.81e-11 63.2
MS.gene25034.t1 MTR_4g022410 52.000 50 24 0 112 161 110 159 2.59e-11 62.0
MS.gene25034.t1 MTR_4g077570 49.020 51 22 2 110 157 6 55 4.20e-11 58.9
MS.gene25034.t1 MTR_1g096780 42.254 71 37 3 90 157 208 277 4.24e-11 63.9
MS.gene25034.t1 MTR_7g116210 35.294 68 44 0 90 157 10 77 4.27e-11 59.7
MS.gene25034.t1 MTR_5g009210 53.333 45 21 0 115 159 315 359 5.41e-11 63.2
MS.gene25034.t1 MTR_3g087900 40.506 79 43 3 114 191 68 143 7.19e-11 60.1
MS.gene25034.t1 MTR_5g009200 53.333 45 21 0 115 159 350 394 7.19e-11 63.2
MS.gene25034.t1 MTR_2g044570 53.191 47 22 0 114 160 82 128 7.84e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25034.t1 AT1G53820 45.545 303 126 10 22 302 19 304 4.38e-67 213
MS.gene25034.t1 AT1G72310 39.936 313 144 6 14 303 11 302 1.44e-65 209
MS.gene25034.t1 AT3G16720 35.593 295 146 9 18 309 21 274 5.61e-40 142
MS.gene25034.t1 AT3G62690 36.667 150 90 2 12 160 13 158 1.49e-25 103
MS.gene25034.t1 AT4G15975 42.241 116 55 3 45 160 18 121 6.91e-24 98.2
MS.gene25034.t1 AT1G23980 36.296 135 81 3 28 157 52 186 3.93e-23 98.6
MS.gene25034.t1 AT5G17600 33.939 165 101 4 30 193 59 216 1.30e-22 97.1
MS.gene25034.t1 AT3G14320 47.368 76 40 0 88 163 61 136 2.22e-22 93.2
MS.gene25034.t1 AT1G49220 47.170 106 45 4 68 166 84 185 2.72e-22 94.0
MS.gene25034.t1 AT2G47560 36.585 164 89 4 1 160 1 153 3.78e-22 93.2
MS.gene25034.t1 AT5G05280 36.306 157 83 4 4 157 13 155 3.89e-22 91.7
MS.gene25034.t1 AT2G42360 34.932 146 80 2 17 160 21 153 3.92e-21 90.5
MS.gene25034.t1 AT2G20030 39.437 142 72 4 23 163 44 172 5.36e-21 92.8
MS.gene25034.t1 AT1G04360 35.570 149 71 6 27 164 49 183 5.95e-21 92.8
MS.gene25034.t1 AT3G18773 41.584 101 56 2 90 188 103 202 2.61e-20 88.2
MS.gene25034.t1 AT3G48030 33.108 148 95 2 27 172 41 186 3.24e-20 89.0
MS.gene25034.t1 AT1G20823 33.766 154 85 5 17 165 20 161 5.07e-20 86.7
MS.gene25034.t1 AT4G40070 35.714 154 80 6 24 171 40 180 5.79e-20 89.0
MS.gene25034.t1 AT1G49210 50.633 79 36 2 90 166 108 185 7.03e-20 87.0
MS.gene25034.t1 AT3G48030 33.108 148 95 2 27 172 119 264 7.05e-20 89.4
MS.gene25034.t1 AT1G49200 48.810 84 40 2 85 166 104 186 7.16e-20 87.0
MS.gene25034.t1 AT5G43420 31.841 201 108 7 16 197 31 221 2.97e-19 87.8
MS.gene25034.t1 AT5G05810 52.000 75 35 1 90 163 120 194 3.28e-19 87.8
MS.gene25034.t1 AT1G76410 33.571 140 78 4 28 165 28 154 3.84e-19 84.0
MS.gene25034.t1 AT3G10910 44.565 92 48 2 90 179 88 178 5.01e-19 83.6
MS.gene25034.t1 AT4G28890 44.792 96 50 2 90 184 97 190 7.86e-19 87.0
MS.gene25034.t1 AT4G30400 42.157 102 52 3 61 157 77 176 1.58e-18 86.3
MS.gene25034.t1 AT1G72200 29.126 206 116 5 3 191 41 233 1.67e-18 85.9
MS.gene25034.t1 AT2G35000 30.882 204 119 8 28 220 51 243 1.92e-18 85.5
MS.gene25034.t1 AT5G40250 34.014 147 73 4 29 157 45 185 2.25e-18 85.1
MS.gene25034.t1 AT4G10150 30.769 130 88 1 28 157 26 153 7.78e-18 81.6
MS.gene25034.t1 AT5G10380 52.857 70 32 1 89 157 108 177 8.85e-18 82.4
MS.gene25034.t1 AT3G03550 38.182 110 55 2 91 187 134 243 9.55e-18 83.2
MS.gene25034.t1 AT2G18650 45.977 87 44 3 78 161 87 173 1.76e-17 82.8
MS.gene25034.t1 AT3G19140 41.176 85 44 1 79 157 37 121 2.01e-17 78.2
MS.gene25034.t1 AT5G01880 45.833 72 38 1 88 158 77 148 2.13e-17 78.6
MS.gene25034.t1 AT3G05200 37.500 104 56 3 90 184 99 202 3.28e-17 82.0
MS.gene25034.t1 AT2G27940 31.343 134 89 1 28 158 50 183 4.80e-17 79.3
MS.gene25034.t1 AT1G33480 40.698 86 48 1 72 157 60 142 8.52e-17 79.3
MS.gene25034.t1 AT4G33565 36.792 106 66 1 80 184 181 286 9.05e-17 80.5
MS.gene25034.t1 AT5G42200 31.544 149 80 4 15 157 14 146 9.71e-17 76.6
MS.gene25034.t1 AT4G10160 36.842 114 61 4 88 200 70 173 1.21e-16 78.2
MS.gene25034.t1 AT2G17450 40.777 103 48 3 71 165 55 152 1.27e-16 77.0
MS.gene25034.t1 AT1G72220 29.861 144 94 3 21 162 86 224 1.57e-16 80.1
MS.gene25034.t1 AT4G09110 32.374 139 72 3 29 163 51 171 1.59e-16 79.0
MS.gene25034.t1 AT4G17905 44.186 86 45 2 80 162 117 202 2.10e-16 79.0
MS.gene25034.t1 AT4G17905 44.186 86 45 2 80 162 143 228 2.67e-16 79.0
MS.gene25034.t1 AT1G49230 40.659 91 51 2 90 178 104 193 2.67e-16 77.0
MS.gene25034.t1 AT4G09120 38.144 97 55 2 71 163 76 171 2.82e-16 79.0
MS.gene25034.t1 AT2G42350 30.496 141 82 1 17 157 18 142 3.27e-16 76.6
MS.gene25034.t1 AT5G27420 41.463 82 44 2 90 167 95 176 6.76e-16 77.8
MS.gene25034.t1 AT1G32361 36.364 99 56 3 65 163 111 202 7.11e-16 77.4
MS.gene25034.t1 AT5G57750 30.464 151 86 5 17 158 25 165 7.95e-16 75.5
MS.gene25034.t1 AT2G34990 43.056 72 37 1 90 157 89 160 1.12e-15 77.0
MS.gene25034.t1 AT2G34990 43.056 72 37 1 90 157 67 138 1.17e-15 76.6
MS.gene25034.t1 AT4G09130 37.363 91 53 2 78 164 79 169 1.84e-15 76.6
MS.gene25034.t1 AT1G74410 42.500 80 44 2 83 160 142 221 2.16e-15 74.3
MS.gene25034.t1 AT2G35420 32.031 128 77 4 33 157 25 145 3.27e-15 74.7
MS.gene25034.t1 AT5G07040 39.189 74 42 1 88 158 64 137 3.48e-15 72.4
MS.gene25034.t1 AT4G17920 39.394 99 55 2 90 184 81 178 7.27e-15 73.9
MS.gene25034.t1 AT3G18930 36.559 93 47 2 80 160 109 201 1.45e-14 74.3
MS.gene25034.t1 AT3G18930 36.559 93 47 2 80 160 109 201 1.45e-14 74.3
MS.gene25034.t1 AT3G18930 36.559 93 47 2 80 160 109 201 1.45e-14 74.3
MS.gene25034.t1 AT1G22500 40.217 92 50 2 76 162 75 166 1.71e-14 73.9
MS.gene25034.t1 AT5G58580 39.286 112 60 3 90 193 111 222 3.54e-14 72.4
MS.gene25034.t1 AT4G09100 44.000 75 38 1 90 160 56 130 4.02e-14 68.6
MS.gene25034.t1 AT5G46650 34.507 142 72 7 28 157 25 157 4.47e-14 72.0
MS.gene25034.t1 AT1G35330 41.667 72 38 1 90 157 100 171 1.20e-13 70.9
MS.gene25034.t1 AT3G61550 31.034 116 60 4 56 158 71 179 5.55e-13 67.4
MS.gene25034.t1 AT4G24015 40.449 89 49 3 89 175 79 165 1.08e-12 65.9
MS.gene25034.t1 AT3G60220 38.372 86 46 2 80 158 75 160 1.17e-12 68.2
MS.gene25034.t1 AT2G46493 45.833 48 26 0 111 158 134 181 1.79e-12 65.5
MS.gene25034.t1 AT3G11110 30.000 150 88 3 17 164 22 156 2.05e-12 64.7
MS.gene25034.t1 AT2G46160 30.612 98 57 2 72 158 84 181 2.56e-12 65.5
MS.gene25034.t1 AT2G46160 30.612 98 57 2 72 158 84 181 2.56e-12 65.5
MS.gene25034.t1 AT4G35480 44.828 58 32 0 108 165 106 163 2.64e-12 65.5
MS.gene25034.t1 AT5G06490 34.615 78 43 1 89 158 95 172 3.18e-12 65.1
MS.gene25034.t1 AT2G44578 58.140 43 18 0 115 157 70 112 6.74e-12 62.8
MS.gene25034.t1 AT5G66070 54.348 46 21 0 113 158 174 219 7.66e-12 64.3
MS.gene25034.t1 AT5G66070 54.348 46 21 0 113 158 174 219 7.66e-12 64.3
MS.gene25034.t1 AT5G53110 36.735 98 54 3 69 158 276 373 8.32e-12 65.9
MS.gene25034.t1 AT2G46494 47.917 48 25 0 111 158 312 359 8.39e-12 65.9
MS.gene25034.t1 AT2G17730 40.278 72 39 1 90 157 167 238 1.15e-11 64.3
MS.gene25034.t1 AT5G66070 55.556 45 20 0 113 157 198 242 1.28e-11 63.9
MS.gene25034.t1 AT2G34000 33.333 99 58 2 69 159 37 135 1.35e-11 62.0
MS.gene25034.t1 AT1G28040 45.833 48 26 0 111 158 249 296 2.22e-11 63.9
MS.gene25034.t1 AT5G47610 41.270 63 35 1 100 160 90 152 2.32e-11 62.0
MS.gene25034.t1 AT3G60966 60.465 43 17 0 115 157 62 104 7.66e-11 59.7
MS.gene25034.t1 AT2G44581 53.488 43 20 0 115 157 70 112 8.03e-11 59.7
MS.gene25034.t1 AT2G46495 45.833 48 26 0 111 158 288 335 9.28e-11 62.4
MS.gene25034.t1 AT1G22670 36.047 86 51 3 90 172 205 289 9.44e-11 62.8
MS.gene25034.t1 AT3G20395 36.111 72 45 1 90 161 147 217 9.53e-11 61.2
MS.gene25034.t1 AT2G46495 45.833 48 26 0 111 158 316 363 9.76e-11 62.8

Find 53 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTTGAATCAGTACTATT+TGG 0.192512 8.1:-77356412 None:intergenic
TCTTAAGTCTTTGCCTGTTT+TGG 0.218884 8.1:+77356198 MS.gene25034:CDS
TGAAACCCATGATTGCATTT+TGG 0.226368 8.1:-77356307 None:intergenic
CGTCGACGACGGCGGTTTCT+TGG 0.237232 8.1:-77356103 None:intergenic
ATTCTACTTGTCCTCTTTGT+AGG 0.257676 8.1:+77356362 MS.gene25034:CDS
ATTTATATGCAAAGTGGTTT+TGG 0.278266 8.1:+77356050 MS.gene25034:CDS
TCCTTCAACTCCAAGTTCTA+TGG 0.291895 8.1:+77356828 MS.gene25034:CDS
AAATTTCCCTACAAATGTTT+TGG 0.305638 8.1:+77356504 MS.gene25034:CDS
TGAGGATTTCAAAGATGGTT+TGG 0.341504 8.1:+77356231 MS.gene25034:CDS
ACACAAGTTAGTTCTTCTTT+TGG 0.347982 8.1:+77356544 MS.gene25034:CDS
GGTCAAGATCCTGTTGTCTA+TGG 0.356257 8.1:+77356145 MS.gene25034:CDS
GTCTATGGAAGTCACCAAAT+TGG 0.362498 8.1:+77356160 MS.gene25034:CDS
AAAGGGTCAGGTGATGTATT+AGG 0.362982 8.1:+77355938 MS.gene25034:CDS
CAAGATTGAGATCATTTAAG+AGG 0.369842 8.1:+77356779 MS.gene25034:CDS
TCATCATCATCATCTACTTT+AGG 0.389863 8.1:+77356613 MS.gene25034:CDS
AGTAGTAGATGTAATGGAAA+TGG 0.393771 8.1:+77356652 MS.gene25034:CDS
CCCCAAACCAAAACATTTGT+AGG 0.395435 8.1:-77356511 None:intergenic
TCTAATACAGTTGAAATAAC+AGG 0.400393 8.1:+77355965 MS.gene25034:CDS
GCAACTGCAACTGCAACAAA+AGG 0.404413 8.1:+77355920 MS.gene25034:CDS
TCTACTTTAGGTAGTGATAA+TGG 0.408867 8.1:+77356625 MS.gene25034:CDS
CATTGTCACCTTCTGATGAT+AGG 0.417548 8.1:+77356722 MS.gene25034:CDS
TGTCTTTGTGATGTTGTTGA+AGG 0.438454 8.1:+77356265 MS.gene25034:CDS
TCCATAGAACTTGGAGTTGA+AGG 0.444903 8.1:-77356829 None:intergenic
ATTTAGATTGTATTGATATG+TGG 0.449698 8.1:+77356329 MS.gene25034:CDS
CGACGGCGGTTTCTTGGTTG+AGG 0.455007 8.1:-77356097 None:intergenic
GGTAATAGTAGTAGATGTAA+TGG 0.462276 8.1:+77356646 MS.gene25034:CDS
TGGTGACTTCCATAGACAAC+AGG 0.469321 8.1:-77356154 None:intergenic
TTTGGTGTTTCTTTGGAAGA+AGG 0.471878 8.1:+77356562 MS.gene25034:CDS
TGTTCCACATCCATAGAACT+TGG 0.475183 8.1:-77356838 None:intergenic
AACTCCAAGTTCTATGGATG+TGG 0.479911 8.1:+77356834 MS.gene25034:CDS
CCAAATAGTACTGATTCAAA+TGG 0.506743 8.1:+77356412 MS.gene25034:CDS
GTCACTAGCACTATTAGCTC+TGG 0.507539 8.1:-77356864 None:intergenic
GAAAATTTGAGTCATGTGAA+TGG 0.511787 8.1:+77356469 MS.gene25034:CDS
GAACTAAACTCAGTTGGTAA+TGG 0.517099 8.1:-77356694 None:intergenic
AAACAAACCGCCGTCGACGA+CGG 0.534947 8.1:-77356114 None:intergenic
CCCAAACCAAAACATTTGTA+GGG 0.536138 8.1:-77356510 None:intergenic
TGCTTCATTTATATGCAAAG+TGG 0.539115 8.1:+77356044 MS.gene25034:CDS
GCAACTGCAACAAAAGGGTC+AGG 0.546145 8.1:+77355926 MS.gene25034:CDS
CAACTGCAACTGCAACAAAA+GGG 0.562538 8.1:+77355921 MS.gene25034:CDS
GATCTCAATCTTGTTGCCAT+TGG 0.564352 8.1:-77356769 None:intergenic
GAAACTAAATTCCTACAAAG+AGG 0.564972 8.1:-77356373 None:intergenic
GAGGAAGAACTAAACTCAGT+TGG 0.568064 8.1:-77356700 None:intergenic
ATGTAATGGAAATGGAATGT+TGG 0.576201 8.1:+77356660 MS.gene25034:CDS
CAAGAAACCGCCGTCGTCGA+CGG 0.576823 8.1:+77356104 MS.gene25034:CDS
AGTTCTATGGATGTGGAACA+AGG 0.581073 8.1:+77356841 MS.gene25034:CDS
AGATGAAATGAAATCACCAA+TGG 0.606642 8.1:+77356753 MS.gene25034:CDS
ATCATCAGAAGGTGACAATG+AGG 0.643860 8.1:-77356719 None:intergenic
TCAACACACCTATCATCAGA+AGG 0.647870 8.1:-77356730 None:intergenic
AATGTGTTAGTCTCATCAGA+AGG 0.648849 8.1:+77356445 MS.gene25034:CDS
CAAACCGCCGTCGACGACGG+CGG 0.650391 8.1:-77356111 None:intergenic
GAAACCGCCGTCGTCGACGG+CGG 0.652134 8.1:+77356107 MS.gene25034:CDS
TAGACAACAGGATCTTGACC+AGG 0.656018 8.1:-77356142 None:intergenic
GATGATGATGATGTTGAACA+AGG 0.694801 8.1:-77356601 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCAATAATTTTTCTATTCA+TGG + chr8.1:77356003-77356022 MS.gene25034:CDS 20.0%
!!! ATTTAGATTGTATTGATATG+TGG + chr8.1:77356329-77356348 MS.gene25034:CDS 20.0%
! AAAAATGAGGATTTCAAAGA+TGG + chr8.1:77356226-77356245 MS.gene25034:CDS 25.0%
! TCTAATACAGTTGAAATAAC+AGG + chr8.1:77355965-77355984 MS.gene25034:CDS 25.0%
! TGAAATAACAGGAAAAATCA+TGG + chr8.1:77355976-77355995 MS.gene25034:CDS 25.0%
!! AAATTTCCCTACAAATGTTT+TGG + chr8.1:77356504-77356523 MS.gene25034:CDS 25.0%
!! ATTTATATGCAAAGTGGTTT+TGG + chr8.1:77356050-77356069 MS.gene25034:CDS 25.0%
!! TTTTACCAAAATGCAATCAT+GGG + chr8.1:77356302-77356321 MS.gene25034:CDS 25.0%
!!! TCATTTTTGAAAACCAAAAC+AGG - chr8.1:77356214-77356233 None:intergenic 25.0%
!!! TGTTTTGGTTTTCAAAAATG+AGG + chr8.1:77356213-77356232 MS.gene25034:CDS 25.0%
AGATGAAATGAAATCACCAA+TGG + chr8.1:77356753-77356772 MS.gene25034:CDS 30.0%
CAAGATTGAGATCATTTAAG+AGG + chr8.1:77356779-77356798 MS.gene25034:CDS 30.0%
CCATTTGAATCAGTACTATT+TGG - chr8.1:77356415-77356434 None:intergenic 30.0%
GAAAATTTGAGTCATGTGAA+TGG + chr8.1:77356469-77356488 MS.gene25034:CDS 30.0%
GAAACTAAATTCCTACAAAG+AGG - chr8.1:77356376-77356395 None:intergenic 30.0%
GGTAATAGTAGTAGATGTAA+TGG + chr8.1:77356646-77356665 MS.gene25034:CDS 30.0%
TCATCATCATCATCTACTTT+AGG + chr8.1:77356613-77356632 MS.gene25034:CDS 30.0%
TCTACTTTAGGTAGTGATAA+TGG + chr8.1:77356625-77356644 MS.gene25034:CDS 30.0%
TGCTTCATTTATATGCAAAG+TGG + chr8.1:77356044-77356063 MS.gene25034:CDS 30.0%
TTGTTGAAGGAGAAAAAACT+AGG + chr8.1:77356278-77356297 MS.gene25034:CDS 30.0%
! AGTAGTAGATGTAATGGAAA+TGG + chr8.1:77356652-77356671 MS.gene25034:CDS 30.0%
! ATGTAATGGAAATGGAATGT+TGG + chr8.1:77356660-77356679 MS.gene25034:CDS 30.0%
! CTTTTACCAAAATGCAATCA+TGG + chr8.1:77356301-77356320 MS.gene25034:CDS 30.0%
!! ACACAAGTTAGTTCTTCTTT+TGG + chr8.1:77356544-77356563 MS.gene25034:CDS 30.0%
!! CCAAATAGTACTGATTCAAA+TGG + chr8.1:77356412-77356431 MS.gene25034:CDS 30.0%
!!! TTCTTCTTTTGGTGTTTCTT+TGG + chr8.1:77356555-77356574 MS.gene25034:CDS 30.0%
!!! TTTTAGCTTCAAGTCCAATT+TGG - chr8.1:77356177-77356196 None:intergenic 30.0%
ATTCTACTTGTCCTCTTTGT+AGG + chr8.1:77356362-77356381 MS.gene25034:CDS 35.0%
CCCAAACCAAAACATTTGTA+GGG - chr8.1:77356513-77356532 None:intergenic 35.0%
TGAGGATTTCAAAGATGGTT+TGG + chr8.1:77356231-77356250 MS.gene25034:CDS 35.0%
TGTCTTTGTGATGTTGTTGA+AGG + chr8.1:77356265-77356284 MS.gene25034:CDS 35.0%
! AATGTGTTAGTCTCATCAGA+AGG + chr8.1:77356445-77356464 MS.gene25034:CDS 35.0%
! GATGATGATGATGTTGAACA+AGG - chr8.1:77356604-77356623 None:intergenic 35.0%
! TCTTAAGTCTTTGCCTGTTT+TGG + chr8.1:77356198-77356217 MS.gene25034:CDS 35.0%
! TGAAACCCATGATTGCATTT+TGG - chr8.1:77356310-77356329 None:intergenic 35.0%
!! GAACTAAACTCAGTTGGTAA+TGG - chr8.1:77356697-77356716 None:intergenic 35.0%
!! TTTGGTGTTTCTTTGGAAGA+AGG + chr8.1:77356562-77356581 MS.gene25034:CDS 35.0%
!!! CCCTACAAATGTTTTGGTTT+GGG + chr8.1:77356510-77356529 MS.gene25034:CDS 35.0%
!!! CCTACAAATGTTTTGGTTTG+GGG + chr8.1:77356511-77356530 MS.gene25034:CDS 35.0%
!!! CTACAAATGTTTTGGTTTGG+GGG + chr8.1:77356512-77356531 MS.gene25034:CDS 35.0%
!!! TATATGCAAAGTGGTTTTGG+TGG + chr8.1:77356053-77356072 MS.gene25034:CDS 35.0%
!!! TCCCTACAAATGTTTTGGTT+TGG + chr8.1:77356509-77356528 MS.gene25034:CDS 35.0%
AAAGGGTCAGGTGATGTATT+AGG + chr8.1:77355938-77355957 MS.gene25034:CDS 40.0%
AACTCCAAGTTCTATGGATG+TGG + chr8.1:77356834-77356853 MS.gene25034:CDS 40.0%
AGTTCTATGGATGTGGAACA+AGG + chr8.1:77356841-77356860 MS.gene25034:CDS 40.0%
ATCATCAGAAGGTGACAATG+AGG - chr8.1:77356722-77356741 None:intergenic 40.0%
CAACTGCAACTGCAACAAAA+GGG + chr8.1:77355921-77355940 MS.gene25034:CDS 40.0%
CATTGTCACCTTCTGATGAT+AGG + chr8.1:77356722-77356741 MS.gene25034:CDS 40.0%
CCCCAAACCAAAACATTTGT+AGG - chr8.1:77356514-77356533 None:intergenic 40.0%
GAGGAAGAACTAAACTCAGT+TGG - chr8.1:77356703-77356722 None:intergenic 40.0%
GATCTCAATCTTGTTGCCAT+TGG - chr8.1:77356772-77356791 None:intergenic 40.0%
GTCTATGGAAGTCACCAAAT+TGG + chr8.1:77356160-77356179 MS.gene25034:CDS 40.0%
TCAACACACCTATCATCAGA+AGG - chr8.1:77356733-77356752 None:intergenic 40.0%
TCCATAGAACTTGGAGTTGA+AGG - chr8.1:77356832-77356851 None:intergenic 40.0%
TCCTTCAACTCCAAGTTCTA+TGG + chr8.1:77356828-77356847 MS.gene25034:CDS 40.0%
TGTTCCACATCCATAGAACT+TGG - chr8.1:77356841-77356860 None:intergenic 40.0%
GCAACTGCAACTGCAACAAA+AGG + chr8.1:77355920-77355939 MS.gene25034:CDS 45.0%
GGTCAAGATCCTGTTGTCTA+TGG + chr8.1:77356145-77356164 MS.gene25034:CDS 45.0%
TAGACAACAGGATCTTGACC+AGG - chr8.1:77356145-77356164 None:intergenic 45.0%
TGGTGACTTCCATAGACAAC+AGG - chr8.1:77356157-77356176 None:intergenic 45.0%
GCAACTGCAACAAAAGGGTC+AGG + chr8.1:77355926-77355945 MS.gene25034:CDS 50.0%
!!! GTGGTTTTGGTGGCGTTTTG+AGG + chr8.1:77356063-77356082 MS.gene25034:CDS 50.0%
AAACAAACCGCCGTCGACGA+CGG - chr8.1:77356117-77356136 None:intergenic 55.0%
!!! CGGCGGTTTGTTTTTGCTCC+TGG + chr8.1:77356124-77356143 MS.gene25034:CDS 55.0%
CAAGAAACCGCCGTCGTCGA+CGG + chr8.1:77356104-77356123 MS.gene25034:CDS 60.0%
CGACGGCGGTTTCTTGGTTG+AGG - chr8.1:77356100-77356119 None:intergenic 60.0%
CGTCGACGACGGCGGTTTCT+TGG - chr8.1:77356106-77356125 None:intergenic 65.0%
CAAACCGCCGTCGACGACGG+CGG - chr8.1:77356114-77356133 None:intergenic 70.0%
GAAACCGCCGTCGTCGACGG+CGG + chr8.1:77356107-77356126 MS.gene25034:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 77355914 77356873 77355914 ID=MS.gene25034
chr8.1 mRNA 77355914 77356873 77355914 ID=MS.gene25034.t1;Parent=MS.gene25034
chr8.1 exon 77355914 77356873 77355914 ID=MS.gene25034.t1.exon1;Parent=MS.gene25034.t1
chr8.1 CDS 77355914 77356873 77355914 ID=cds.MS.gene25034.t1;Parent=MS.gene25034.t1
Gene Sequence

>MS.gene25034

ATGGGTGCAACTGCAACTGCAACAAAAGGGTCAGGTGATGTATTAGGTGATTCTAATACAGTTGAAATAACAGGAAAAATCATGGTTTTAGCAATAATTTTTCTATTCATGGTTGTTATGTTTGTTCTAATGCTTCATTTATATGCAAAGTGGTTTTGGTGGCGTTTTGAGGAAAGAACTGTTCCTCAACCAAGAAACCGCCGTCGTCGACGGCGGTTTGTTTTTGCTCCTGGTCAAGATCCTGTTGTCTATGGAAGTCACCAAATTGGACTTGAAGCTAAAATTCTTAAGTCTTTGCCTGTTTTGGTTTTCAAAAATGAGGATTTCAAAGATGGTTTGGAATGTGCTGTTTGTCTTTGTGATGTTGTTGAAGGAGAAAAAACTAGGCTTTTACCAAAATGCAATCATGGGTTTCATTTAGATTGTATTGATATGTGGTTTCAATCTCATTCTACTTGTCCTCTTTGTAGGAATTTAGTTTCTGTTGAATCATGTAAACCAAATAGTACTGATTCAAATGGTGAAGAGATGAATGTGTTAGTCTCATCAGAAGGTGAAAATTTGAGTCATGTGAATGGTTTAGAGTCTTCAAATTTCCCTACAAATGTTTTGGTTTGGGGGAATTCTCAAACACAAGTTAGTTCTTCTTTTGGTGTTTCTTTGGAAGAAGGAAGTTCTCATGAACAACCTTGTTCAACATCATCATCATCATCTACTTTAGGTAGTGATAATGGTAATAGTAGTAGATGTAATGGAAATGGAATGTTGGTGATTGATATACCATTACCAACTGAGTTTAGTTCTTCCTCATTGTCACCTTCTGATGATAGGTGTGTTGAAGATGAAATGAAATCACCAATGGCAACAAGATTGAGATCATTTAAGAGGCTTTTAAGTAGAGACAAAAAGTTTAGTCCTTCAACTCCAAGTTCTATGGATGTGGAACAAGGCCAGAGCTAA
Protein sequence

>MS.gene25034.t1

MGATATATKGSGDVLGDSNTVEITGKIMVLAIIFLFMVVMFVLMLHLYAKWFWWRFEERTVPQPRNRRRRRRFVFAPGQDPVVYGSHQIGLEAKILKSLPVLVFKNEDFKDGLECAVCLCDVVEGEKTRLLPKCNHGFHLDCIDMWFQSHSTCPLCRNLVSVESCKPNSTDSNGEEMNVLVSSEGENLSHVNGLESSNFPTNVLVWGNSQTQVSSSFGVSLEEGSSHEQPCSTSSSSSTLGSDNGNSSRCNGNGMLVIDIPLPTEFSSSSLSPSDDRCVEDEMKSPMATRLRSFKRLLSRDKKFSPSTPSSMDVEQGQS