AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25131


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25131.t1 MTR_8g020770 94.811 212 11 0 1 212 1 212 4.17e-147 408
MS.gene25131.t1 MTR_8g020750 66.667 213 69 2 1 212 1 212 1.82e-96 280
MS.gene25131.t1 MTR_4g010340 62.559 211 72 5 3 212 1 205 6.13e-84 248
MS.gene25131.t1 MTR_8g020630 53.774 212 95 2 1 212 1 209 5.68e-79 235
MS.gene25131.t1 MTR_8g020760 57.746 213 87 3 1 212 1 211 8.06e-79 235
MS.gene25131.t1 MTR_8g020650 57.143 189 79 2 24 212 23 209 1.97e-74 224
MS.gene25131.t1 MTR_0284s0010 52.830 212 97 2 1 212 1 209 4.37e-74 223
MS.gene25131.t1 MTR_2g044040 52.358 212 98 3 1 212 1 209 6.07e-74 223
MS.gene25131.t1 MTR_2g036120 53.081 211 96 3 1 211 1 208 4.73e-72 218
MS.gene25131.t1 MTR_7g011340 57.143 189 79 1 24 212 23 209 5.83e-72 218
MS.gene25131.t1 MTR_3g019710 51.887 212 99 3 1 212 1 209 1.11e-70 214
MS.gene25131.t1 MTR_3g073170 52.093 215 95 5 1 212 1 210 8.26e-70 212
MS.gene25131.t1 MTR_2g019780 52.535 217 93 5 1 212 1 212 1.16e-69 212
MS.gene25131.t1 MTR_8g020670 50.239 209 102 2 4 212 3 209 2.96e-69 211
MS.gene25131.t1 MTR_2g045100 51.456 206 98 2 7 212 6 209 1.52e-68 209
MS.gene25131.t1 MTR_5g031460 49.528 212 104 3 1 212 1 209 2.28e-68 208
MS.gene25131.t1 MTR_3g019650 50.943 212 100 3 1 212 1 208 6.43e-68 207
MS.gene25131.t1 MTR_8g020763 55.191 183 81 1 30 212 9 190 6.64e-68 206
MS.gene25131.t1 MTR_3g019680 51.415 212 99 3 1 212 1 208 9.63e-68 207
MS.gene25131.t1 MTR_8g020610 50.242 207 99 3 7 212 4 207 1.26e-64 199
MS.gene25131.t1 MTR_2g041570 46.009 213 111 3 1 212 1 210 1.72e-63 196
MS.gene25131.t1 MTR_2g041580 45.540 213 112 3 1 212 1 210 1.70e-62 193
MS.gene25131.t1 MTR_2g041550 46.479 213 110 3 1 212 1 210 3.95e-61 190
MS.gene25131.t1 MTR_8g020590 48.341 211 105 4 3 212 1 208 4.05e-61 190
MS.gene25131.t1 MTR_3g019810 46.117 206 102 3 1 206 107 303 3.34e-51 168
MS.gene25131.t1 MTR_4g027415 55.738 122 52 1 24 145 23 142 1.05e-40 135
MS.gene25131.t1 MTR_6g005330 42.135 178 90 4 3 170 1 175 1.37e-30 112
MS.gene25131.t1 MTR_6g005380 41.899 179 90 4 3 170 1 176 1.60e-30 112
MS.gene25131.t1 MTR_6g005310 41.989 181 86 5 3 170 1 175 2.36e-30 111
MS.gene25131.t1 MTR_1g085500 41.317 167 92 4 7 169 9 173 2.53e-30 111
MS.gene25131.t1 MTR_6g005360 41.989 181 86 5 3 170 1 175 2.68e-30 111
MS.gene25131.t1 MTR_7g111240 42.767 159 86 4 24 178 25 182 2.80e-30 111
MS.gene25131.t1 MTR_2g030855 37.245 196 113 4 16 208 19 207 3.07e-30 111
MS.gene25131.t1 MTR_6g005340 41.989 181 86 5 3 170 1 175 6.57e-30 110
MS.gene25131.t1 MTR_3g019610 52.336 107 49 1 4 110 3 107 6.91e-30 107
MS.gene25131.t1 MTR_1g085140 37.113 194 118 3 15 205 20 212 9.51e-30 110
MS.gene25131.t1 MTR_7g111240 46.269 134 67 3 24 156 25 154 1.55e-29 109
MS.gene25131.t1 MTR_5g046410 40.223 179 95 4 1 170 1 176 2.61e-29 108
MS.gene25131.t1 MTR_5g046430 40.223 179 95 4 1 170 1 176 2.61e-29 108
MS.gene25131.t1 MTR_4g017030 40.223 179 95 4 1 170 1 176 2.61e-29 108
MS.gene25131.t1 MTR_2g072560 50.435 115 55 2 4 118 3 115 2.77e-29 106
MS.gene25131.t1 MTR_2g031270 44.000 150 77 3 24 170 28 173 2.87e-29 108
MS.gene25131.t1 MTR_1g079490 39.594 197 108 5 1 188 1 195 2.88e-29 108
MS.gene25131.t1 MTR_4g017050 40.223 179 95 4 1 170 1 176 2.99e-29 108
MS.gene25131.t1 MTR_4g017040 39.665 179 96 4 1 170 1 176 3.78e-29 108
MS.gene25131.t1 MTR_2g019250 40.223 179 95 4 1 170 1 176 1.16e-28 107
MS.gene25131.t1 MTR_1g112200 31.579 209 136 3 4 211 8 210 1.00e-27 104
MS.gene25131.t1 MTR_1g023770 39.521 167 91 3 7 170 10 169 1.15e-27 104
MS.gene25131.t1 MTR_8g007300 44.030 134 72 2 38 170 47 178 2.81e-27 103
MS.gene25131.t1 MTR_1g060120 33.816 207 125 5 11 207 18 222 1.09e-26 102
MS.gene25131.t1 MTR_5g036570 31.937 191 125 4 24 210 32 221 3.44e-26 100
MS.gene25131.t1 MTR_6g005350 36.946 203 115 4 3 195 1 200 2.15e-25 99.0
MS.gene25131.t1 MTR_3g096330 35.754 179 104 3 1 170 553 729 3.43e-25 103
MS.gene25131.t1 MTR_2g030895 38.596 171 97 4 42 208 7 173 3.65e-25 97.1
MS.gene25131.t1 MTR_2g030865 43.609 133 70 3 42 170 7 138 2.26e-23 92.4
MS.gene25131.t1 MTR_2g031300 45.794 107 54 2 42 145 7 112 4.62e-20 83.2
MS.gene25131.t1 MTR_2g096110 34.437 151 79 3 47 197 8 138 1.82e-17 75.9
MS.gene25131.t1 MTR_2g086640 31.818 176 102 7 2 173 7 168 9.11e-17 75.9
MS.gene25131.t1 MTR_4g052770 32.919 161 93 5 34 180 28 187 2.10e-16 74.7
MS.gene25131.t1 MTR_4g052780 32.919 161 93 5 34 180 28 187 4.59e-16 73.9
MS.gene25131.t1 MTR_2g086620 35.172 145 86 5 34 173 28 169 1.19e-15 72.8
MS.gene25131.t1 MTR_2g086630 34.483 145 87 5 34 173 28 169 2.85e-15 71.6
MS.gene25131.t1 MTR_7g029500 52.830 53 25 0 153 205 38 90 2.66e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25131.t1 AT5G20630 49.057 212 105 3 1 211 1 210 1.11e-61 191
MS.gene25131.t1 AT1G72610 49.474 190 94 2 24 212 20 208 2.12e-56 178
MS.gene25131.t1 AT5G39160 39.091 220 119 6 1 209 1 216 7.54e-40 136
MS.gene25131.t1 AT5G39130 38.462 221 123 6 1 209 1 220 1.51e-38 133
MS.gene25131.t1 AT5G39190 38.462 221 123 6 1 209 1 220 1.54e-38 133
MS.gene25131.t1 AT5G39160 38.462 221 123 6 1 209 1 220 2.94e-38 132
MS.gene25131.t1 AT3G04200 38.356 219 124 5 1 209 6 223 1.17e-37 130
MS.gene25131.t1 AT5G38960 41.262 206 101 5 20 209 22 223 1.88e-37 130
MS.gene25131.t1 AT5G26700 42.328 189 102 5 24 209 26 210 4.76e-36 126
MS.gene25131.t1 AT3G05930 42.021 188 106 2 24 208 28 215 7.54e-36 125
MS.gene25131.t1 AT1G02335 38.967 213 118 6 6 209 7 216 5.05e-35 124
MS.gene25131.t1 AT5G39110 45.395 152 76 3 25 170 30 180 1.29e-34 122
MS.gene25131.t1 AT3G62020 42.775 173 88 4 9 175 8 175 1.14e-33 120
MS.gene25131.t1 AT3G05950 46.053 152 76 3 24 170 31 181 1.99e-33 120
MS.gene25131.t1 AT3G04150 44.079 152 79 3 24 170 31 181 1.41e-32 117
MS.gene25131.t1 AT3G04150 46.617 133 66 2 42 170 63 194 1.42e-32 118
MS.gene25131.t1 AT5G39120 40.437 183 90 6 1 170 3 179 1.89e-32 117
MS.gene25131.t1 AT5G39180 37.755 196 107 4 1 186 5 195 2.52e-32 117
MS.gene25131.t1 AT5G39150 39.444 180 94 4 1 170 5 179 7.68e-32 115
MS.gene25131.t1 AT3G04190 44.737 152 78 3 24 170 31 181 2.00e-31 114
MS.gene25131.t1 AT5G38940 39.583 192 108 4 24 208 31 221 3.38e-31 114
MS.gene25131.t1 AT5G38940 39.583 192 108 4 24 208 55 245 5.55e-31 114
MS.gene25131.t1 AT1G18980 36.493 211 128 4 2 209 12 219 6.50e-31 113
MS.gene25131.t1 AT5G39190 35.000 220 110 6 1 209 1 198 6.64e-31 112
MS.gene25131.t1 AT4G14630 45.860 157 70 4 24 170 31 182 8.91e-31 112
MS.gene25131.t1 AT5G39160 35.000 220 110 6 1 209 1 198 9.16e-31 112
MS.gene25131.t1 AT5G61750 37.989 179 95 4 1 170 1 172 5.86e-30 110
MS.gene25131.t1 AT3G62020 45.652 138 69 3 39 175 14 146 8.44e-30 109
MS.gene25131.t1 AT5G38910 37.788 217 117 6 4 208 9 219 2.80e-29 108
MS.gene25131.t1 AT5G38930 38.542 192 110 4 24 208 31 221 4.11e-29 108
MS.gene25131.t1 AT1G09560 39.429 175 101 3 16 186 18 191 1.19e-28 107
MS.gene25131.t1 AT3G04180 36.649 191 113 4 25 208 32 221 4.05e-28 106
MS.gene25131.t1 AT1G74820 44.776 134 70 3 24 156 41 171 5.05e-28 105
MS.gene25131.t1 AT1G18970 35.681 213 128 5 3 209 10 219 6.17e-28 105
MS.gene25131.t1 AT3G04170 42.581 155 74 4 24 170 31 178 5.01e-27 103
MS.gene25131.t1 AT1G10460 36.000 150 94 2 39 186 42 191 5.78e-26 100
MS.gene25131.t1 AT3G10080 35.673 171 103 3 41 208 53 219 8.83e-23 92.4
MS.gene25131.t1 AT3G10080 35.673 171 103 3 41 208 124 290 4.90e-22 91.7
MS.gene25131.t1 AT5G38910 31.308 214 98 6 4 208 9 182 1.68e-14 69.3

Find 31 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAGCTTAAGGTTAATTT+TGG 0.189584 8.4:-72844027 MS.gene25131:CDS
CACTTAATCCTGGTGATGTT+TGG 0.277337 8.4:-72844227 MS.gene25131:CDS
ACTTAATCCTGGTGATGTTT+GGG 0.337157 8.4:-72844226 MS.gene25131:CDS
GAGTTTGTCGACGTGTTTGC+AGG 0.363951 8.4:+72844450 None:intergenic
TCCACAAATTATGAAGCTTA+AGG 0.372687 8.4:-72844037 MS.gene25131:CDS
ATCGTTTCCAGACACCCCTT+CGG 0.376629 8.4:-72844538 MS.gene25131:CDS
TCGTTTCCAGACACCCCTTC+GGG 0.386894 8.4:-72844537 MS.gene25131:CDS
ACCTTAAGCTTCATAATTTG+TGG 0.389226 8.4:+72844036 None:intergenic
GGATAGCCCGAAGGGGTGTC+TGG 0.391633 8.4:+72844531 None:intergenic
ATCAGAATCAATAGCATTTG+AGG 0.419441 8.4:+72844581 None:intergenic
AGAATAGATCTTGGCTTCAA+TGG 0.435930 8.4:-72844354 MS.gene25131:CDS
TTTATACTTAACACTGGTCC+TGG 0.439650 8.4:-72844180 MS.gene25131:CDS
GAATAAGCAACAGCATTTCC+AGG 0.471369 8.4:+72844162 None:intergenic
TTTATGAAAACACTTAATCC+TGG 0.483307 8.4:-72844237 MS.gene25131:CDS
TCTGTAGCAAGAATAGATCT+TGG 0.497563 8.4:-72844363 MS.gene25131:CDS
ATTCAAGGTCAAATGACTGC+TGG 0.516733 8.4:-72844282 MS.gene25131:CDS
ATAAAATGCAAAAGTCCTGT+TGG 0.527650 8.4:+72844198 None:intergenic
GATAAGATACCAGGTCTCAA+TGG 0.527717 8.4:-72844396 MS.gene25131:CDS
ATTATAAATTCGCCTATGCC+AGG 0.531971 8.4:+72844309 None:intergenic
ATACCAGGTCTCAATGGACT+TGG 0.540360 8.4:-72844390 MS.gene25131:CDS
GAATTTATAATCGTCATTCA+AGG 0.543864 8.4:-72844297 MS.gene25131:CDS
GTACCAATGCACACTCATCC+TGG 0.556822 8.4:-72844327 MS.gene25131:CDS
TTAAGCTTCATAATTTGTGG+AGG 0.583672 8.4:+72844039 None:intergenic
ACTTGCATGGATAGCCCGAA+GGG 0.586847 8.4:+72844523 None:intergenic
GACTTGCATGGATAGCCCGA+AGG 0.590826 8.4:+72844522 None:intergenic
GTAAAAGTCGATAAGATACC+AGG 0.594356 8.4:-72844405 MS.gene25131:CDS
ATGCACACTCATCCTGGCAT+AGG 0.628928 8.4:-72844321 MS.gene25131:CDS
ATGCCAGGATGAGTGTGCAT+TGG 0.630970 8.4:+72844324 None:intergenic
TTCGGGCTATCCATGCAAGT+CGG 0.659661 8.4:-72844520 MS.gene25131:CDS
ATACCAAGTCCATTGAGACC+TGG 0.667082 8.4:+72844387 None:intergenic
CTTGCATGGATAGCCCGAAG+GGG 0.715273 8.4:+72844524 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAACTGATTTTTTGTTATT+TGG - chr8.4:72844536-72844555 MS.gene25131:CDS 15.0%
!!! GTTTTCATAAAAACTTTTGT+AGG + chr8.4:72844398-72844417 None:intergenic 20.0%
! GAATTTATAATCGTCATTCA+AGG - chr8.4:72844347-72844366 MS.gene25131:CDS 25.0%
! TTTATGAAAACACTTAATCC+TGG - chr8.4:72844407-72844426 MS.gene25131:CDS 25.0%
!! ATGAAGCTTAAGGTTAATTT+TGG - chr8.4:72844617-72844636 MS.gene25131:CDS 25.0%
!! TTGCATTTTATACTTAACAC+TGG - chr8.4:72844458-72844477 MS.gene25131:CDS 25.0%
ACCTTAAGCTTCATAATTTG+TGG + chr8.4:72844611-72844630 None:intergenic 30.0%
ATAAAATGCAAAAGTCCTGT+TGG + chr8.4:72844449-72844468 None:intergenic 30.0%
ATCAGAATCAATAGCATTTG+AGG + chr8.4:72844066-72844085 None:intergenic 30.0%
TCCACAAATTATGAAGCTTA+AGG - chr8.4:72844607-72844626 MS.gene25131:CDS 30.0%
! AAGCTTAAGGTTAATTTTGG+TGG - chr8.4:72844620-72844639 MS.gene25131:CDS 30.0%
! TTAAGCTTCATAATTTGTGG+AGG + chr8.4:72844608-72844627 None:intergenic 30.0%
!!! TATCTTATCGACTTTTACAG+TGG + chr8.4:72844237-72844256 None:intergenic 30.0%
AGAATAGATCTTGGCTTCAA+TGG - chr8.4:72844290-72844309 MS.gene25131:CDS 35.0%
ATTATAAATTCGCCTATGCC+AGG + chr8.4:72844338-72844357 None:intergenic 35.0%
GTAAAAGTCGATAAGATACC+AGG - chr8.4:72844239-72844258 MS.gene25131:CDS 35.0%
TCTGTAGCAAGAATAGATCT+TGG - chr8.4:72844281-72844300 MS.gene25131:CDS 35.0%
TTTATACTTAACACTGGTCC+TGG - chr8.4:72844464-72844483 MS.gene25131:CDS 35.0%
! AAGAGTAGTCTTTTGAACTG+TGG + chr8.4:72844579-72844598 None:intergenic 35.0%
! ACTTAATCCTGGTGATGTTT+GGG - chr8.4:72844418-72844437 MS.gene25131:CDS 35.0%
! GCTAATGATTTTGTGTTCTC+TGG - chr8.4:72844161-72844180 MS.gene25131:CDS 35.0%
ATTCAAGGTCAAATGACTGC+TGG - chr8.4:72844362-72844381 MS.gene25131:CDS 40.0%
GAATAAGCAACAGCATTTCC+AGG + chr8.4:72844485-72844504 None:intergenic 40.0%
GATAAGATACCAGGTCTCAA+TGG - chr8.4:72844248-72844267 MS.gene25131:CDS 40.0%
! CACTTAATCCTGGTGATGTT+TGG - chr8.4:72844417-72844436 MS.gene25131:CDS 40.0%
! GTCTTTTGAACTGTGGAAGT+TGG + chr8.4:72844572-72844591 None:intergenic 40.0%
!! AAGGTTAATTTTGGTGGCAG+TGG - chr8.4:72844626-72844645 MS.gene25131:CDS 40.0%
!! ACATTGTTTTCCGACTTGCA+TGG + chr8.4:72844137-72844156 None:intergenic 40.0%
!! GATGTTTGGGTTTTTCCAAC+AGG - chr8.4:72844431-72844450 MS.gene25131:CDS 40.0%
ATACCAAGTCCATTGAGACC+TGG + chr8.4:72844260-72844279 None:intergenic 45.0%
GGAAAAACCCAAACATCACC+AGG + chr8.4:72844428-72844447 None:intergenic 45.0%
! ATACCAGGTCTCAATGGACT+TGG - chr8.4:72844254-72844273 MS.gene25131:CDS 45.0%
ACTTGCATGGATAGCCCGAA+GGG + chr8.4:72844124-72844143 None:intergenic 50.0%
ATCGTTTCCAGACACCCCTT+CGG - chr8.4:72844106-72844125 MS.gene25131:CDS 50.0%
ATGCACACTCATCCTGGCAT+AGG - chr8.4:72844323-72844342 MS.gene25131:CDS 50.0%
ATGCCAGGATGAGTGTGCAT+TGG + chr8.4:72844323-72844342 None:intergenic 50.0%
GAGTTTGTCGACGTGTTTGC+AGG + chr8.4:72844197-72844216 None:intergenic 50.0%
GTACCAATGCACACTCATCC+TGG - chr8.4:72844317-72844336 MS.gene25131:CDS 50.0%
TTCGGGCTATCCATGCAAGT+CGG - chr8.4:72844124-72844143 MS.gene25131:CDS 50.0%
CTTGCATGGATAGCCCGAAG+GGG + chr8.4:72844123-72844142 None:intergenic 55.0%
GACTTGCATGGATAGCCCGA+AGG + chr8.4:72844125-72844144 None:intergenic 55.0%
TCGTTTCCAGACACCCCTTC+GGG - chr8.4:72844107-72844126 MS.gene25131:CDS 55.0%
!! GGATAGCCCGAAGGGGTGTC+TGG + chr8.4:72844116-72844135 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 72844014 72844652 72844014 ID=MS.gene25131
chr8.4 mRNA 72844014 72844652 72844014 ID=MS.gene25131.t1;Parent=MS.gene25131
chr8.4 exon 72844014 72844652 72844014 ID=MS.gene25131.t1.exon1;Parent=MS.gene25131.t1
chr8.4 CDS 72844014 72844652 72844014 ID=cds.MS.gene25131.t1;Parent=MS.gene25131.t1
Gene Sequence

>MS.gene25131

ATGAGGATGATTCAAACTATTCTTTTCTTTTTTGCTCTTATCTCATATACCTCAAATGCTATTGATTCTGATGATTTCTGTGTAGCAGACTTATCGTTTCCAGACACCCCTTCGGGCTATCCATGCAAGTCGGAAAACAATGTAACAGCTAATGATTTTGTGTTCTCTGGCTTAAATATACCTGCAAACACGTCGACAAACTCTTTTAAAGTTGCATTTACCACTGTAAAAGTCGATAAGATACCAGGTCTCAATGGACTTGGTATCTCTGTAGCAAGAATAGATCTTGGCTTCAATGGAAGTGTACCAATGCACACTCATCCTGGCATAGGCGAATTTATAATCGTCATTCAAGGTCAAATGACTGCTGGATTTATCACACCTACAAAAGTTTTTATGAAAACACTTAATCCTGGTGATGTTTGGGTTTTTCCAACAGGACTTTTGCATTTTATACTTAACACTGGTCCTGGAAATGCTGTTGCTTATTCTGCATATGCTAGCTCAAATTTTAACACTCATTTAACTGATTTTTTGTTATTTGGTAACAACTTACCAACTTCCACAGTTCAAAAGACTACTCTTCTTGATCCTCCACAAATTATGAAGCTTAAGGTTAATTTTGGTGGCAGTGGCTAG

Protein sequence

>MS.gene25131.t1

MRMIQTILFFFALISYTSNAIDSDDFCVADLSFPDTPSGYPCKSENNVTANDFVFSGLNIPANTSTNSFKVAFTTVKVDKIPGLNGLGISVARIDLGFNGSVPMHTHPGIGEFIIVIQGQMTAGFITPTKVFMKTLNPGDVWVFPTGLLHFILNTGPGNAVAYSAYASSNFNTHLTDFLLFGNNLPTSTVQKTTLLDPPQIMKLKVNFGGSG