AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25132


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25132.t1 MTR_8g020760 83.568 213 33 1 1 213 1 211 1.21e-121 343
MS.gene25132.t1 MTR_4g010340 75.355 211 46 2 3 213 1 205 6.54e-109 311
MS.gene25132.t1 MTR_8g020763 80.220 182 35 1 33 213 9 190 3.71e-101 291
MS.gene25132.t1 MTR_8g020630 60.563 213 80 2 1 213 1 209 4.53e-89 261
MS.gene25132.t1 MTR_2g019780 63.426 216 72 4 1 213 1 212 4.41e-86 253
MS.gene25132.t1 MTR_8g020650 58.216 213 85 1 1 213 1 209 8.68e-86 253
MS.gene25132.t1 MTR_2g044040 59.155 213 83 2 1 213 1 209 2.27e-85 251
MS.gene25132.t1 MTR_2g045100 61.033 213 79 2 1 213 1 209 2.00e-84 249
MS.gene25132.t1 MTR_3g073170 63.551 214 73 3 1 213 1 210 2.19e-84 249
MS.gene25132.t1 MTR_8g020770 59.070 215 83 4 1 213 1 212 1.19e-83 247
MS.gene25132.t1 MTR_2g036120 59.434 212 82 2 1 212 1 208 1.14e-82 244
MS.gene25132.t1 MTR_8g020670 57.971 207 84 1 7 213 6 209 1.88e-81 241
MS.gene25132.t1 MTR_0284s0010 58.216 213 85 2 1 213 1 209 9.37e-81 240
MS.gene25132.t1 MTR_8g020750 59.070 215 83 4 1 213 1 212 5.77e-79 235
MS.gene25132.t1 MTR_7g011340 61.702 188 72 0 26 213 22 209 6.87e-79 235
MS.gene25132.t1 MTR_3g019710 57.746 213 86 2 1 213 1 209 3.10e-78 233
MS.gene25132.t1 MTR_3g019680 55.238 210 90 1 4 213 3 208 4.74e-74 223
MS.gene25132.t1 MTR_3g019650 55.072 207 89 1 7 213 6 208 8.01e-74 222
MS.gene25132.t1 MTR_5g031460 53.991 213 94 2 1 213 1 209 2.26e-73 221
MS.gene25132.t1 MTR_8g020590 48.804 209 101 3 7 213 4 208 1.88e-65 201
MS.gene25132.t1 MTR_2g041570 47.907 215 105 3 1 213 1 210 9.79e-64 197
MS.gene25132.t1 MTR_2g041580 47.442 215 106 3 1 213 1 210 6.11e-63 194
MS.gene25132.t1 MTR_2g041550 47.442 215 106 3 1 213 1 210 1.71e-61 191
MS.gene25132.t1 MTR_8g020610 49.282 209 102 3 7 213 1 207 4.44e-61 190
MS.gene25132.t1 MTR_3g019810 48.544 206 96 3 1 206 107 302 1.22e-56 182
MS.gene25132.t1 MTR_4g027415 61.983 121 46 0 26 146 22 142 2.05e-45 147
MS.gene25132.t1 MTR_2g072560 56.198 121 45 2 1 119 1 115 4.04e-36 124
MS.gene25132.t1 MTR_1g079490 36.406 217 130 3 1 209 1 217 5.91e-35 123
MS.gene25132.t1 MTR_3g019610 54.955 111 42 2 1 109 1 105 5.93e-32 112
MS.gene25132.t1 MTR_2g031270 36.598 194 114 4 22 209 23 213 1.52e-31 114
MS.gene25132.t1 MTR_6g005330 36.111 216 130 4 1 209 1 215 5.62e-31 113
MS.gene25132.t1 MTR_6g005360 35.648 216 131 4 1 209 1 215 2.35e-30 111
MS.gene25132.t1 MTR_6g005310 36.792 212 124 5 7 209 5 215 1.00e-29 110
MS.gene25132.t1 MTR_4g017050 35.945 217 128 5 1 208 1 215 2.65e-29 108
MS.gene25132.t1 MTR_1g112200 35.938 192 110 7 26 212 27 210 2.80e-29 108
MS.gene25132.t1 MTR_5g046410 35.945 217 128 5 1 208 1 215 2.89e-29 108
MS.gene25132.t1 MTR_5g046430 35.945 217 128 5 1 208 1 215 2.89e-29 108
MS.gene25132.t1 MTR_4g017030 35.945 217 128 5 1 208 1 215 2.89e-29 108
MS.gene25132.t1 MTR_6g005340 36.321 212 125 5 7 209 5 215 5.13e-29 108
MS.gene25132.t1 MTR_1g085500 35.514 214 122 6 7 210 8 215 7.91e-29 107
MS.gene25132.t1 MTR_1g085140 37.696 191 111 4 26 210 28 216 1.41e-28 107
MS.gene25132.t1 MTR_1g023770 35.417 192 112 4 24 209 24 209 2.01e-28 106
MS.gene25132.t1 MTR_2g030855 37.634 186 106 4 23 204 23 202 2.05e-28 106
MS.gene25132.t1 MTR_7g111240 44.595 148 79 3 25 169 23 170 2.39e-28 106
MS.gene25132.t1 MTR_1g060120 31.163 215 139 3 4 209 7 221 3.04e-28 106
MS.gene25132.t1 MTR_7g111240 45.270 148 75 4 25 169 23 167 4.95e-28 105
MS.gene25132.t1 MTR_4g017040 35.023 217 130 5 1 208 1 215 5.57e-28 105
MS.gene25132.t1 MTR_8g007300 38.150 173 95 6 8 172 11 179 7.18e-28 105
MS.gene25132.t1 MTR_2g019250 35.023 217 130 5 1 208 1 215 1.78e-27 104
MS.gene25132.t1 MTR_6g005380 40.476 168 93 3 11 172 11 177 2.24e-27 103
MS.gene25132.t1 MTR_2g030895 35.465 172 104 4 45 209 7 178 4.54e-25 97.1
MS.gene25132.t1 MTR_6g005350 33.019 212 133 5 6 209 4 214 1.00e-23 94.4
MS.gene25132.t1 MTR_2g030865 35.465 172 104 4 45 209 7 178 1.82e-23 92.8
MS.gene25132.t1 MTR_5g036570 31.414 191 126 4 26 211 31 221 2.40e-23 93.6
MS.gene25132.t1 MTR_3g096330 33.146 178 102 3 41 203 598 773 2.70e-23 97.4
MS.gene25132.t1 MTR_2g031300 44.340 106 55 2 45 146 7 112 3.52e-20 83.6
MS.gene25132.t1 MTR_2g086620 31.373 204 129 5 15 209 3 204 1.93e-18 80.1
MS.gene25132.t1 MTR_2g086630 31.373 204 129 5 15 209 3 204 2.73e-18 79.7
MS.gene25132.t1 MTR_2g086640 29.412 204 135 5 14 209 1 203 3.06e-18 79.7
MS.gene25132.t1 MTR_2g096110 36.913 149 72 5 52 198 10 138 3.98e-18 77.8
MS.gene25132.t1 MTR_7g029500 71.698 53 15 0 154 206 38 90 5.84e-17 73.2
MS.gene25132.t1 MTR_4g052770 34.146 164 103 3 51 209 42 205 2.55e-16 74.7
MS.gene25132.t1 MTR_4g052780 34.146 164 103 3 51 209 42 205 6.53e-16 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25132.t1 AT1G72610 48.958 192 96 2 24 213 17 208 1.06e-60 189
MS.gene25132.t1 AT5G20630 45.327 214 111 3 1 212 1 210 2.36e-56 178
MS.gene25132.t1 AT5G38960 42.000 200 99 5 24 210 28 223 1.82e-37 130
MS.gene25132.t1 AT3G05950 39.614 207 117 4 11 209 15 221 8.81e-34 120
MS.gene25132.t1 AT3G04200 36.574 216 127 5 3 210 10 223 2.17e-32 117
MS.gene25132.t1 AT5G39190 36.232 207 124 4 12 210 14 220 2.35e-32 117
MS.gene25132.t1 AT5G39160 35.437 206 123 4 12 210 14 216 2.93e-32 116
MS.gene25132.t1 AT5G39160 35.749 207 125 4 12 210 14 220 5.70e-32 116
MS.gene25132.t1 AT5G39130 35.749 207 125 4 12 210 14 220 1.61e-31 115
MS.gene25132.t1 AT5G38940 38.144 194 112 4 24 209 28 221 7.36e-31 113
MS.gene25132.t1 AT5G38940 38.144 194 112 4 24 209 52 245 1.06e-30 113
MS.gene25132.t1 AT5G26700 36.181 199 118 4 17 210 16 210 1.46e-30 112
MS.gene25132.t1 AT3G05930 35.096 208 130 3 7 209 8 215 1.86e-30 112
MS.gene25132.t1 AT4G14630 37.209 215 118 5 8 209 12 222 3.60e-30 111
MS.gene25132.t1 AT5G39110 35.211 213 129 4 6 209 8 220 1.05e-29 110
MS.gene25132.t1 AT1G09560 41.615 161 92 2 26 184 28 188 1.46e-29 109
MS.gene25132.t1 AT1G02335 35.263 190 119 3 25 210 27 216 1.81e-29 109
MS.gene25132.t1 AT5G38910 35.349 215 123 5 7 209 9 219 2.88e-29 108
MS.gene25132.t1 AT5G61750 42.647 136 72 3 41 172 40 173 3.48e-29 108
MS.gene25132.t1 AT3G04150 32.536 209 133 4 13 213 17 225 4.72e-29 108
MS.gene25132.t1 AT5G38930 37.113 194 114 4 24 209 28 221 5.24e-29 108
MS.gene25132.t1 AT3G04190 35.204 196 119 4 15 202 19 214 1.14e-28 107
MS.gene25132.t1 AT1G18980 37.037 189 113 4 26 210 33 219 2.05e-28 106
MS.gene25132.t1 AT3G62020 34.742 213 124 6 9 210 8 216 2.54e-28 106
MS.gene25132.t1 AT3G04150 34.659 176 108 3 45 213 63 238 2.93e-28 107
MS.gene25132.t1 AT3G04180 34.975 203 124 4 15 209 19 221 4.44e-28 105
MS.gene25132.t1 AT5G39180 34.722 216 130 5 4 209 5 219 6.36e-28 105
MS.gene25132.t1 AT3G04170 35.849 212 115 8 11 209 15 218 1.38e-27 104
MS.gene25132.t1 AT5G39120 34.722 216 130 5 4 209 5 219 1.65e-27 104
MS.gene25132.t1 AT5G39150 34.722 216 130 5 4 209 5 219 1.82e-27 104
MS.gene25132.t1 AT3G10080 31.156 199 131 2 17 209 21 219 5.58e-26 100
MS.gene25132.t1 AT3G10080 33.503 197 111 5 26 209 101 290 1.17e-25 101
MS.gene25132.t1 AT1G18970 31.925 213 139 4 2 210 9 219 1.89e-25 99.0
MS.gene25132.t1 AT5G39190 32.195 205 113 3 12 210 14 198 2.21e-25 98.6
MS.gene25132.t1 AT3G62020 35.429 175 108 4 41 210 13 187 3.41e-25 97.8
MS.gene25132.t1 AT5G39160 31.707 205 114 3 12 210 14 198 5.46e-25 97.4
MS.gene25132.t1 AT1G10460 37.107 159 93 5 26 177 23 181 3.28e-24 95.9
MS.gene25132.t1 AT1G74820 42.857 133 72 3 27 157 41 171 1.13e-23 94.7
MS.gene25132.t1 AT5G38910 29.717 212 102 5 7 209 9 182 1.94e-15 71.6

Find 36 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACTTGATTATCTATTATT+TGG 0.155412 8.4:-72847437 MS.gene25132:CDS
ACCAGTTTACCTGGTCTTAA+TGG 0.209440 8.4:-72847725 MS.gene25132:CDS
ACAAAATGCAAAAGCCCTTC+TGG 0.264438 8.4:+72847527 None:intergenic
TCTGCTGGAAGAGGAGATTT+TGG 0.267961 8.4:-72847692 MS.gene25132:CDS
GAATTAGTTATCGTGGTTAA+AGG 0.280928 8.4:-72847626 MS.gene25132:CDS
GTGGTTAATCTAACATTAAA+AGG 0.285906 8.4:+72847758 None:intergenic
GTTGGAAATGCTATTGCTTT+TGG 0.309862 8.4:-72847491 MS.gene25132:CDS
GACAGAAGAAGTTGCAGTTT+GGG 0.342150 8.4:+72847907 None:intergenic
AAAGGGTCTATTGTGTTTCC+AGG 0.347883 8.4:+72847776 None:intergenic
TGACAGAAGAAGTTGCAGTT+TGG 0.368327 8.4:+72847906 None:intergenic
TCCATTAAGACCAGGTAAAC+TGG 0.370975 8.4:+72847724 None:intergenic
ATAAAGCTTAAGAATCAGTT+TGG 0.389749 8.4:-72847356 MS.gene25132:CDS
GGATAGCCTGAAGGAGTGTT+TGG 0.395462 8.4:+72847854 None:intergenic
GTGTTCTCTGGCTTTGTACC+TGG 0.400296 8.4:-72847794 MS.gene25132:CDS
CAGCTTCCAAACACTCCTTC+AGG 0.418699 8.4:-72847860 MS.gene25132:CDS
TTTGTAGTTAACTCTGGTGT+TGG 0.425062 8.4:-72847509 MS.gene25132:CDS
GCATTTGTCACCAGTTTACC+TGG 0.439631 8.4:-72847734 MS.gene25132:CDS
AAGAATCAGTTTGGTGGTAG+TGG 0.444437 8.4:-72847347 MS.gene25132:CDS
TAATAAATCCAGCAGTCAAT+TGG 0.460927 8.4:+72847603 None:intergenic
TTACCTGGTCTTAATGGACT+TGG 0.465576 8.4:-72847719 MS.gene25132:CDS
TAGTAGCTCAAACCCTAAAA+CGG 0.473073 8.4:-72847462 MS.gene25132:CDS
AATGGACTTGGTATATCTGC+TGG 0.482284 8.4:-72847707 MS.gene25132:CDS
ATAACTAATTCAGTTGCATC+AGG 0.492749 8.4:+72847638 None:intergenic
GTTAAAGGCCAATTGACTGC+TGG 0.522198 8.4:-72847611 MS.gene25132:CDS
ACACTTGTTTCTGACTTGCA+TGG 0.533166 8.4:+72847833 None:intergenic
CTTGGTATATCTGCTGGAAG+AGG 0.540629 8.4:-72847701 MS.gene25132:CDS
TGGTTAATCTAACATTAAAA+GGG 0.555953 8.4:+72847759 None:intergenic
TATTTGAAAACTGTTAAACC+TGG 0.562860 8.4:-72847566 MS.gene25132:CDS
AAGCTTAAGAATCAGTTTGG+TGG 0.578020 8.4:-72847353 MS.gene25132:CDS
TGCAACTGAATTAGTTATCG+TGG 0.584807 8.4:-72847633 MS.gene25132:CDS
AGAAGAAGTTGCAGTTTGGG+AGG 0.599718 8.4:+72847910 None:intergenic
GCATCAGGATGAGTGAGCAT+TGG 0.603045 8.4:+72847653 None:intergenic
GACTTGCATGGATAGCCTGA+AGG 0.608627 8.4:+72847845 None:intergenic
TTCTGTCAATGATTTCTGTG+TGG 0.614592 8.4:-72847891 MS.gene25132:CDS
ATACCAAGTCCATTAAGACC+AGG 0.671415 8.4:+72847716 None:intergenic
TAAACTGGTGACAAATGCAG+TGG 0.677227 8.4:+72847739 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTTGATTATCTATTATT+TGG - chr8.4:72847868-72847887 MS.gene25132:CDS 15.0%
!! TGGTTAATCTAACATTAAAA+GGG + chr8.4:72847549-72847568 None:intergenic 20.0%
!!! GTTTTCAAATAAACTTTTGT+TGG + chr8.4:72847730-72847749 None:intergenic 20.0%
! ATAAAGCTTAAGAATCAGTT+TGG - chr8.4:72847949-72847968 MS.gene25132:CDS 25.0%
! GTGGTTAATCTAACATTAAA+AGG + chr8.4:72847550-72847569 None:intergenic 25.0%
! TATTTGAAAACTGTTAAACC+TGG - chr8.4:72847739-72847758 MS.gene25132:CDS 25.0%
!! AATCAAGTATTTCCGTTTTA+GGG + chr8.4:72847858-72847877 None:intergenic 25.0%
!! ATATTGTAGTTTTTCCAGAA+GGG - chr8.4:72847764-72847783 MS.gene25132:CDS 25.0%
!! TAATCAAGTATTTCCGTTTT+AGG + chr8.4:72847859-72847878 None:intergenic 25.0%
ATAACTAATTCAGTTGCATC+AGG + chr8.4:72847670-72847689 None:intergenic 30.0%
GAATTAGTTATCGTGGTTAA+AGG - chr8.4:72847679-72847698 MS.gene25132:CDS 30.0%
TAATAAATCCAGCAGTCAAT+TGG + chr8.4:72847705-72847724 None:intergenic 30.0%
! GATATTGTAGTTTTTCCAGA+AGG - chr8.4:72847763-72847782 MS.gene25132:CDS 30.0%
! TTGCATTTTGTAGTTAACTC+TGG - chr8.4:72847790-72847809 MS.gene25132:CDS 30.0%
!! AGAGGAGATTTTGGTATAAA+TGG - chr8.4:72847622-72847641 MS.gene25132:CDS 30.0%
AAGCTTAAGAATCAGTTTGG+TGG - chr8.4:72847952-72847971 MS.gene25132:CDS 35.0%
GGAAAAACTACAATATCACC+AGG + chr8.4:72847760-72847779 None:intergenic 35.0%
TAGTAGCTCAAACCCTAAAA+CGG - chr8.4:72847843-72847862 MS.gene25132:CDS 35.0%
TGCAACTGAATTAGTTATCG+TGG - chr8.4:72847672-72847691 MS.gene25132:CDS 35.0%
TTCTGTCAATGATTTCTGTG+TGG - chr8.4:72847414-72847433 MS.gene25132:CDS 35.0%
TTTGTAGTTAACTCTGGTGT+TGG - chr8.4:72847796-72847815 MS.gene25132:CDS 35.0%
! GTTGGAAATGCTATTGCTTT+TGG - chr8.4:72847814-72847833 MS.gene25132:CDS 35.0%
AAAGGGTCTATTGTGTTTCC+AGG + chr8.4:72847532-72847551 None:intergenic 40.0%
ACAAAATGCAAAAGCCCTTC+TGG + chr8.4:72847781-72847800 None:intergenic 40.0%
ATACCAAGTCCATTAAGACC+AGG + chr8.4:72847592-72847611 None:intergenic 40.0%
GACAGAAGAAGTTGCAGTTT+GGG + chr8.4:72847401-72847420 None:intergenic 40.0%
TAAACTGGTGACAAATGCAG+TGG + chr8.4:72847569-72847588 None:intergenic 40.0%
TCCATTAAGACCAGGTAAAC+TGG + chr8.4:72847584-72847603 None:intergenic 40.0%
TGACAGAAGAAGTTGCAGTT+TGG + chr8.4:72847402-72847421 None:intergenic 40.0%
! AAGAATCAGTTTGGTGGTAG+TGG - chr8.4:72847958-72847977 MS.gene25132:CDS 40.0%
! AATGGACTTGGTATATCTGC+TGG - chr8.4:72847598-72847617 MS.gene25132:CDS 40.0%
! GCAGATGATTTTGTGTTCTC+TGG - chr8.4:72847499-72847518 MS.gene25132:CDS 40.0%
!! ACACTTGTTTCTGACTTGCA+TGG + chr8.4:72847475-72847494 None:intergenic 40.0%
!! ACCAGTTTACCTGGTCTTAA+TGG - chr8.4:72847580-72847599 MS.gene25132:CDS 40.0%
!! TTACCTGGTCTTAATGGACT+TGG - chr8.4:72847586-72847605 MS.gene25132:CDS 40.0%
AGAAGAAGTTGCAGTTTGGG+AGG + chr8.4:72847398-72847417 None:intergenic 45.0%
CTTGGTATATCTGCTGGAAG+AGG - chr8.4:72847604-72847623 MS.gene25132:CDS 45.0%
GCATTTGTCACCAGTTTACC+TGG - chr8.4:72847571-72847590 MS.gene25132:CDS 45.0%
GTTAAAGGCCAATTGACTGC+TGG - chr8.4:72847694-72847713 MS.gene25132:CDS 45.0%
! TCTGCTGGAAGAGGAGATTT+TGG - chr8.4:72847613-72847632 MS.gene25132:CDS 45.0%
CAGCTTCCAAACACTCCTTC+AGG - chr8.4:72847445-72847464 MS.gene25132:CDS 50.0%
GACTTGCATGGATAGCCTGA+AGG + chr8.4:72847463-72847482 None:intergenic 50.0%
GCATCAGGATGAGTGAGCAT+TGG + chr8.4:72847655-72847674 None:intergenic 50.0%
!! GGATAGCCTGAAGGAGTGTT+TGG + chr8.4:72847454-72847473 None:intergenic 50.0%
!! GTGTTCTCTGGCTTTGTACC+TGG - chr8.4:72847511-72847530 MS.gene25132:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 72847343 72847984 72847343 ID=MS.gene25132
chr8.4 mRNA 72847343 72847984 72847343 ID=MS.gene25132.t1;Parent=MS.gene25132
chr8.4 exon 72847343 72847984 72847343 ID=MS.gene25132.t1.exon1;Parent=MS.gene25132.t1
chr8.4 CDS 72847343 72847984 72847343 ID=cds.MS.gene25132.t1;Parent=MS.gene25132.t1
Gene Sequence

>MS.gene25132

ATGGGGATCATTCACATTATTCTTTTCTATTTTTCTCTTCTCTCATCGTATACCTCCCAAACTGCAACTTCTTCTGTCAATGATTTCTGTGTGGCAGACTTACAGCTTCCAAACACTCCTTCAGGCTATCCATGCAAGTCAGAAACAAGTGTAACAGCAGATGATTTTGTGTTCTCTGGCTTTGTACCTGGAAACACAATAGACCCTTTTAATGTTAGATTAACCACTGCATTTGTCACCAGTTTACCTGGTCTTAATGGACTTGGTATATCTGCTGGAAGAGGAGATTTTGGTATAAATGGAACTGTACCAATGCTCACTCATCCTGATGCAACTGAATTAGTTATCGTGGTTAAAGGCCAATTGACTGCTGGATTTATTACACCAACAAAAGTTTATTTGAAAACTGTTAAACCTGGTGATATTGTAGTTTTTCCAGAAGGGCTTTTGCATTTTGTAGTTAACTCTGGTGTTGGAAATGCTATTGCTTTTGGTTTTTTTAGTAGCTCAAACCCTAAAACGGAAATACTTGATTATCTATTATTTGGCAACGATTTATCAACTTCAATAATTGCAAACACTACTTTACTTGATGTGTCACAAATTATAAAGCTTAAGAATCAGTTTGGTGGTAGTGGCTAA

Protein sequence

>MS.gene25132.t1

MGIIHIILFYFSLLSSYTSQTATSSVNDFCVADLQLPNTPSGYPCKSETSVTADDFVFSGFVPGNTIDPFNVRLTTAFVTSLPGLNGLGISAGRGDFGINGTVPMLTHPDATELVIVVKGQLTAGFITPTKVYLKTVKPGDIVVFPEGLLHFVVNSGVGNAIAFGFFSSSNPKTEILDYLLFGNDLSTSIIANTTLLDVSQIIKLKNQFGGSG