Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25519.t1 | XP_004510012.1 | 92.3 | 155 | 12 | 0 | 1 | 155 | 144 | 298 | 5.60E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25519.t1 | E9Q349 | 37.5 | 152 | 89 | 3 | 8 | 155 | 264 | 413 | 7.5e-24 | 111.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25519.t1 | A0A1S2YU82 | 92.3 | 155 | 12 | 0 | 1 | 155 | 144 | 298 | 4.0e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061040 | MS.gene25519 | -0.802536 | 5.35E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25519.t1 | MTR_8g467040 | 90.323 | 155 | 15 | 0 | 1 | 155 | 140 | 294 | 6.66e-101 | 296 |
| MS.gene25519.t1 | MTR_1g072740 | 31.081 | 148 | 88 | 4 | 1 | 144 | 314 | 451 | 3.75e-18 | 80.5 |
| MS.gene25519.t1 | MTR_4g102520 | 31.507 | 146 | 93 | 3 | 1 | 142 | 315 | 457 | 5.12e-18 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25519.t1 | AT5G54520 | 71.613 | 155 | 44 | 0 | 1 | 155 | 181 | 335 | 1.67e-78 | 240 |
| MS.gene25519.t1 | AT1G10580 | 33.108 | 148 | 85 | 4 | 1 | 144 | 303 | 440 | 2.00e-18 | 81.3 |
Find 31 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTGCTGGGATGGACAATT+TGG | 0.150720 | 8.2:-42448504 | None:intergenic |
| AATTCAAATATCTTCCTTTC+TGG | 0.153427 | 8.2:-42448263 | MS.gene25519:CDS |
| CATAACTATAATCGAAATTT+AGG | 0.181559 | 8.2:-42448188 | MS.gene25519:CDS |
| AGGAAGATATTTGAATTATC+TGG | 0.183670 | 8.2:+42448269 | None:intergenic |
| CAAAGGGACAAATCAAACTA+TGG | 0.288706 | 8.2:-42448235 | MS.gene25519:CDS |
| GTGAGAATTCTATTATCGTT+TGG | 0.344806 | 8.2:-42448088 | MS.gene25519:CDS |
| TGAGAATTCTATTATCGTTT+GGG | 0.353439 | 8.2:-42448087 | MS.gene25519:CDS |
| ATTCAAATATCTTCCTTTCT+GGG | 0.354275 | 8.2:-42448262 | MS.gene25519:CDS |
| ATTTCTTCTAGTGACGTATC+TGG | 0.385677 | 8.2:-42448122 | MS.gene25519:CDS |
| TGGACAATTTGGTTTGCATA+TGG | 0.393563 | 8.2:-42448493 | MS.gene25519:CDS |
| CAGCAGTGAAAGATGTAAAA+TGG | 0.401012 | 8.2:-42448418 | MS.gene25519:CDS |
| ACAAGTACCGTTGTCTAATC+AGG | 0.413649 | 8.2:-42448054 | MS.gene25519:CDS |
| AGGTTGGTTGATGTTGAAAA+GGG | 0.433049 | 8.2:-42448347 | MS.gene25519:CDS |
| GGAAGATATTTGAATTATCT+GGG | 0.436810 | 8.2:+42448270 | None:intergenic |
| AAGGTTGGTTGATGTTGAAA+AGG | 0.438941 | 8.2:-42448348 | MS.gene25519:CDS |
| TGGTTTGCATATGGAACGTA+TGG | 0.473476 | 8.2:-42448484 | MS.gene25519:CDS |
| TTCAAAGAAGATCAAATGGC+TGG | 0.481015 | 8.2:-42448308 | MS.gene25519:CDS |
| GGGAACTTCTTGCTTTCTTG+TGG | 0.481727 | 8.2:-42448386 | MS.gene25519:CDS |
| TTCAAATATCTTCCTTTCTG+GGG | 0.504458 | 8.2:-42448261 | MS.gene25519:CDS |
| TGAAAAGGGGATGGAAACTC+AGG | 0.548181 | 8.2:-42448333 | MS.gene25519:CDS |
| AAAGGGACAAATCAAACTAT+GGG | 0.552104 | 8.2:-42448234 | MS.gene25519:CDS |
| TCAAATATCTTCCTTTCTGG+GGG | 0.557329 | 8.2:-42448260 | MS.gene25519:CDS |
| GGTTGATGTTGAAAAGGGGA+TGG | 0.571477 | 8.2:-42448342 | MS.gene25519:CDS |
| CATCTTTCACTGCTGCATTG+TGG | 0.587898 | 8.2:+42448426 | None:intergenic |
| GTGGATATGATTGCACATCA+AGG | 0.600456 | 8.2:-42448367 | MS.gene25519:CDS |
| ATATGATTGCACATCAAGGT+TGG | 0.628197 | 8.2:-42448363 | MS.gene25519:CDS |
| GATGTAAAATGGTCTCAACA+AGG | 0.630392 | 8.2:-42448407 | MS.gene25519:CDS |
| ATGTAAAATGGTCTCAACAA+GGG | 0.656310 | 8.2:-42448406 | MS.gene25519:CDS |
| GGTTGGTTGATGTTGAAAAG+GGG | 0.664123 | 8.2:-42448346 | MS.gene25519:CDS |
| AGATATTTGAATTATCTGGG+TGG | 0.709902 | 8.2:+42448273 | None:intergenic |
| TATCGTACCTGATTAGACAA+CGG | 0.715508 | 8.2:+42448047 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTTGAGTTTACAAAAAAT+GGG | - | chr8.2:42448386-42448405 | MS.gene25519:CDS | 20.0% |
| !! | CATAACTATAATCGAAATTT+AGG | - | chr8.2:42448352-42448371 | MS.gene25519:CDS | 20.0% |
| ! | AATTCAAATATCTTCCTTTC+TGG | - | chr8.2:42448277-42448296 | MS.gene25519:CDS | 25.0% |
| ! | AGGAAGATATTTGAATTATC+TGG | + | chr8.2:42448274-42448293 | None:intergenic | 25.0% |
| ! | ATTCAAATATCTTCCTTTCT+GGG | - | chr8.2:42448278-42448297 | MS.gene25519:CDS | 25.0% |
| ! | GATGTTGAGTTTACAAAAAA+TGG | - | chr8.2:42448385-42448404 | MS.gene25519:CDS | 25.0% |
| ! | GGAAGATATTTGAATTATCT+GGG | + | chr8.2:42448273-42448292 | None:intergenic | 25.0% |
| ! | TGAGAATTCTATTATCGTTT+GGG | - | chr8.2:42448453-42448472 | MS.gene25519:CDS | 25.0% |
| AAAGGGACAAATCAAACTAT+GGG | - | chr8.2:42448306-42448325 | MS.gene25519:CDS | 30.0% | |
| AGATATTTGAATTATCTGGG+TGG | + | chr8.2:42448270-42448289 | None:intergenic | 30.0% | |
| ATGTAAAATGGTCTCAACAA+GGG | - | chr8.2:42448134-42448153 | MS.gene25519:CDS | 30.0% | |
| GTGAGAATTCTATTATCGTT+TGG | - | chr8.2:42448452-42448471 | MS.gene25519:CDS | 30.0% | |
| TTCAAATATCTTCCTTTCTG+GGG | - | chr8.2:42448279-42448298 | MS.gene25519:CDS | 30.0% | |
| ! | GGTTTTCAAAGAAGATCAAA+TGG | - | chr8.2:42448228-42448247 | MS.gene25519:CDS | 30.0% |
| ATATGATTGCACATCAAGGT+TGG | - | chr8.2:42448177-42448196 | MS.gene25519:CDS | 35.0% | |
| ATTTCTTCTAGTGACGTATC+TGG | - | chr8.2:42448418-42448437 | MS.gene25519:CDS | 35.0% | |
| CAAAGGGACAAATCAAACTA+TGG | - | chr8.2:42448305-42448324 | MS.gene25519:CDS | 35.0% | |
| CAGCAGTGAAAGATGTAAAA+TGG | - | chr8.2:42448122-42448141 | MS.gene25519:CDS | 35.0% | |
| GATGTAAAATGGTCTCAACA+AGG | - | chr8.2:42448133-42448152 | MS.gene25519:CDS | 35.0% | |
| TATCGTACCTGATTAGACAA+CGG | + | chr8.2:42448496-42448515 | None:intergenic | 35.0% | |
| TCAAATATCTTCCTTTCTGG+GGG | - | chr8.2:42448280-42448299 | MS.gene25519:CDS | 35.0% | |
| TTCAAAGAAGATCAAATGGC+TGG | - | chr8.2:42448232-42448251 | MS.gene25519:CDS | 35.0% | |
| ! | AAGGTTGGTTGATGTTGAAA+AGG | - | chr8.2:42448192-42448211 | MS.gene25519:CDS | 35.0% |
| ! | AGGTTGGTTGATGTTGAAAA+GGG | - | chr8.2:42448193-42448212 | MS.gene25519:CDS | 35.0% |
| !! | TGGACAATTTGGTTTGCATA+TGG | - | chr8.2:42448047-42448066 | MS.gene25519:CDS | 35.0% |
| GTGGATATGATTGCACATCA+AGG | - | chr8.2:42448173-42448192 | MS.gene25519:CDS | 40.0% | |
| ! | ACAAGTACCGTTGTCTAATC+AGG | - | chr8.2:42448486-42448505 | MS.gene25519:CDS | 40.0% |
| ! | GGTTGGTTGATGTTGAAAAG+GGG | - | chr8.2:42448194-42448213 | MS.gene25519:CDS | 40.0% |
| ! | TGGTTTGCATATGGAACGTA+TGG | - | chr8.2:42448056-42448075 | MS.gene25519:CDS | 40.0% |
| CATCTTTCACTGCTGCATTG+TGG | + | chr8.2:42448117-42448136 | None:intergenic | 45.0% | |
| GGGAACTTCTTGCTTTCTTG+TGG | - | chr8.2:42448154-42448173 | MS.gene25519:CDS | 45.0% | |
| TGAAAAGGGGATGGAAACTC+AGG | - | chr8.2:42448207-42448226 | MS.gene25519:CDS | 45.0% | |
| ! | GGTTGATGTTGAAAAGGGGA+TGG | - | chr8.2:42448198-42448217 | MS.gene25519:CDS | 45.0% |
| ! | TTCCTTTCTGGGGGATCAAA+GGG | - | chr8.2:42448289-42448308 | MS.gene25519:CDS | 45.0% |
| ! | CTTCCTTTCTGGGGGATCAA+AGG | - | chr8.2:42448288-42448307 | MS.gene25519:CDS | 50.0% |
| GTCCCTTTGATCCCCCAGAA+AGG | + | chr8.2:42448294-42448313 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 42448046 | 42448516 | 42448046 | ID=MS.gene25519 |
| chr8.2 | mRNA | 42448046 | 42448516 | 42448046 | ID=MS.gene25519.t1;Parent=MS.gene25519 |
| chr8.2 | exon | 42448046 | 42448516 | 42448046 | ID=MS.gene25519.t1.exon1;Parent=MS.gene25519.t1 |
| chr8.2 | CDS | 42448046 | 42448516 | 42448046 | ID=cds.MS.gene25519.t1;Parent=MS.gene25519.t1 |
| Gene Sequence |
| Protein sequence |
