AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25863


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25863.t1 MTR_3g479520 76.687 163 14 1 1 139 1 163 8.36e-85 256
MS.gene25863.t1 MTR_3g479620 66.875 160 29 1 12 147 3 162 7.27e-74 218
MS.gene25863.t1 MTR_3g079480 63.855 166 35 2 7 147 12 177 7.32e-69 216
MS.gene25863.t1 MTR_3g479490 60.625 160 39 1 12 147 3 162 7.98e-63 200
MS.gene25863.t1 MTR_3g479470 58.642 162 42 2 12 149 10 170 1.44e-56 184
MS.gene25863.t1 MTR_5g464810 48.447 161 58 2 10 145 7 167 7.40e-47 158
MS.gene25863.t1 MTR_5g064240 48.447 161 58 2 10 145 7 167 7.40e-47 158
MS.gene25863.t1 MTR_5g464760 48.750 160 57 2 11 145 8 167 1.07e-46 157
MS.gene25863.t1 MTR_0036s0220 46.584 161 61 2 10 145 7 167 2.54e-45 154
MS.gene25863.t1 MTR_2g008210 44.966 149 61 2 12 139 8 156 5.60e-42 145
MS.gene25863.t1 MTR_6g014195 42.484 153 59 2 10 133 5 157 1.40e-37 127
MS.gene25863.t1 MTR_6g014040 40.462 173 72 3 7 149 2 173 5.29e-37 132
MS.gene25863.t1 MTR_4g094220 41.176 153 64 1 13 139 5 157 8.73e-37 131
MS.gene25863.t1 MTR_6g014250 42.282 149 60 2 11 133 6 154 1.93e-36 130
MS.gene25863.t1 MTR_6g014270 42.763 152 56 2 10 130 2 153 6.87e-36 129
MS.gene25863.t1 MTR_0475s0020 57.895 95 37 1 7 98 2 96 8.44e-36 123
MS.gene25863.t1 MTR_6g014290 38.710 155 69 2 11 139 6 160 8.86e-36 129
MS.gene25863.t1 MTR_3g008010 41.176 153 64 2 13 139 8 160 2.00e-35 127
MS.gene25863.t1 MTR_6g014186 39.216 153 64 2 10 133 2 154 3.39e-35 126
MS.gene25863.t1 MTR_0199s0010 40.645 155 65 3 11 139 6 159 1.25e-34 122
MS.gene25863.t1 MTR_6g014220 42.667 150 60 2 11 134 6 155 5.90e-34 124
MS.gene25863.t1 MTR_0288s0020 38.462 169 73 3 11 149 6 173 1.89e-33 122
MS.gene25863.t1 MTR_8g007585 40.645 155 65 3 11 139 6 159 3.70e-33 120
MS.gene25863.t1 MTR_6g014020 36.310 168 78 2 7 145 2 169 1.04e-31 117
MS.gene25863.t1 MTR_6g014150 38.462 169 74 3 10 149 2 169 2.10e-31 117
MS.gene25863.t1 MTR_6g013440 40.506 158 65 2 11 139 7 164 2.76e-31 116
MS.gene25863.t1 MTR_8g059425 36.145 166 79 1 7 145 2 167 6.36e-31 115
MS.gene25863.t1 MTR_6g014187 40.645 155 63 2 11 136 6 160 8.16e-31 115
MS.gene25863.t1 MTR_6g014310 41.667 156 62 2 13 139 9 164 1.91e-30 114
MS.gene25863.t1 MTR_6g014170 41.333 150 62 2 11 134 6 155 2.84e-30 114
MS.gene25863.t1 MTR_6g013960 38.854 157 67 2 7 134 2 158 3.06e-30 114
MS.gene25863.t1 MTR_6g014200 40.237 169 71 3 10 149 2 169 4.41e-30 113
MS.gene25863.t1 MTR_6g014300 38.365 159 69 2 9 138 6 164 7.08e-30 112
MS.gene25863.t1 MTR_7g011470 41.830 153 60 2 10 133 5 157 9.86e-29 109
MS.gene25863.t1 MTR_6g014240 41.135 141 68 3 12 137 4 144 1.05e-28 109
MS.gene25863.t1 MTR_7g011470 41.830 153 60 2 10 133 5 157 1.18e-28 109
MS.gene25863.t1 MTR_6g013450 54.255 94 41 1 7 98 2 95 5.12e-28 107
MS.gene25863.t1 MTR_6g011340 40.127 157 65 2 7 134 2 158 6.37e-27 104
MS.gene25863.t1 MTR_6g014050 57.955 88 35 1 13 98 9 96 8.39e-27 104
MS.gene25863.t1 MTR_6g013990 56.667 90 37 1 11 98 6 95 1.46e-26 103
MS.gene25863.t1 MTR_5g058320 51.064 94 44 1 7 98 2 95 6.21e-26 102
MS.gene25863.t1 MTR_6g013920 36.842 152 68 2 7 130 2 153 1.11e-20 87.4
MS.gene25863.t1 MTR_6g013900 34.839 155 67 2 7 130 2 153 1.72e-19 84.0
MS.gene25863.t1 MTR_5g031860 34.356 163 82 5 8 147 5 165 3.77e-19 83.2
MS.gene25863.t1 MTR_7g013220 35.135 148 67 4 14 132 4 151 1.84e-18 80.9
MS.gene25863.t1 MTR_5g031830 33.333 153 77 5 6 135 3 153 1.03e-17 79.0
MS.gene25863.t1 MTR_7g013200 34.043 141 67 4 14 132 4 140 1.07e-17 79.0
MS.gene25863.t1 MTR_5g031840 35.135 148 70 6 12 135 2 147 3.32e-17 77.4
MS.gene25863.t1 MTR_7g013260 33.333 141 67 3 14 132 5 140 6.24e-16 73.9
MS.gene25863.t1 MTR_7g013240 32.624 141 68 3 14 132 5 140 6.74e-16 73.6
MS.gene25863.t1 MTR_7g013180 28.000 150 84 4 14 139 4 153 3.62e-14 68.9
MS.gene25863.t1 MTR_3g069690 29.371 143 71 4 17 138 10 143 4.19e-12 62.8
MS.gene25863.t1 MTR_6g038240 27.465 142 75 3 11 130 7 142 7.19e-12 62.0
MS.gene25863.t1 MTR_5g098960 31.613 155 81 5 12 145 4 154 3.35e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25863.t1 AT1G22380 46.875 160 61 1 10 145 8 167 1.93e-47 160
MS.gene25863.t1 AT1G22360 46.753 154 58 1 9 138 4 157 5.73e-47 158
MS.gene25863.t1 AT1G22360 46.753 154 58 1 9 138 4 157 5.75e-47 158
MS.gene25863.t1 AT1G22370 47.020 151 56 1 12 138 10 160 1.17e-46 157
MS.gene25863.t1 AT1G22400 44.099 161 66 1 9 145 7 167 1.52e-46 157
MS.gene25863.t1 AT1G22340 42.857 161 68 1 9 145 7 167 5.94e-43 148
MS.gene25863.t1 AT1G78270 41.463 164 71 2 10 149 8 170 9.47e-42 145
MS.gene25863.t1 AT3G02100 30.323 155 82 3 5 133 3 157 4.69e-17 77.0
MS.gene25863.t1 AT3G46660 37.500 144 58 5 11 129 7 143 1.12e-16 75.9
MS.gene25863.t1 AT3G46650 34.014 147 68 4 11 135 6 145 4.52e-15 71.2
MS.gene25863.t1 AT5G38010 31.034 145 66 2 11 129 6 142 1.33e-14 70.1
MS.gene25863.t1 AT3G21560 29.448 163 76 3 14 143 11 167 1.44e-14 70.1
MS.gene25863.t1 AT3G46680 33.566 143 63 5 11 129 5 139 1.86e-13 66.6
MS.gene25863.t1 AT3G46720 34.286 140 62 4 12 129 6 137 4.42e-13 65.9
MS.gene25863.t1 AT2G36970 28.221 163 85 5 11 143 6 166 5.04e-13 65.5
MS.gene25863.t1 AT3G46670 32.432 148 67 5 11 133 2 141 5.48e-13 65.5
MS.gene25863.t1 AT2G28080 31.126 151 76 5 19 143 22 170 5.80e-13 65.5
MS.gene25863.t1 AT3G46690 32.857 140 65 5 12 129 6 138 7.69e-13 65.1
MS.gene25863.t1 AT4G15490 31.373 153 81 4 15 143 8 160 1.18e-12 64.7
MS.gene25863.t1 AT5G59580 33.566 143 65 5 13 133 7 141 2.00e-12 63.9
MS.gene25863.t1 AT5G59590 31.724 145 68 4 12 133 7 143 8.90e-12 62.0
MS.gene25863.t1 AT3G11340 27.389 157 85 4 11 147 5 152 1.92e-11 60.8
MS.gene25863.t1 AT2G26480 32.061 131 63 3 17 129 10 132 2.48e-11 60.8
MS.gene25863.t1 AT4G15500 30.000 150 85 4 14 143 8 157 5.68e-11 59.7

Find 30 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGAACTGCGGGCTTTGC+TGG 0.244090 3.2:-69905160 MS.gene25863:CDS
TGGAAGAGTGGCAAAAGATT+TGG 0.286279 3.2:-69905140 MS.gene25863:CDS
TGCTTCTGCTTGTGGTTTGT+TGG 0.295992 3.2:-69905092 MS.gene25863:CDS
GGAAGAGTGGCAAAAGATTT+GGG 0.303715 3.2:-69905139 MS.gene25863:CDS
ACCTTTGCAACGAAAGAGTT+TGG 0.313608 3.2:+69905440 None:intergenic
TACGAGATGAGCTTGTTAAC+AGG 0.360862 3.2:-69905058 MS.gene25863:CDS
GGATTCAAGAACTAGTGTTT+TGG 0.364043 3.2:-69905117 MS.gene25863:CDS
ATAGTCTCAAACTGAAAATC+TGG 0.364820 3.2:+69905336 None:intergenic
GCTTCTGCTTGTGGTTTGTT+GGG 0.367296 3.2:-69905091 MS.gene25863:CDS
AAACGTTTGATTAAATCTCT+TGG 0.397744 3.2:-69905381 MS.gene25863:CDS
ACATGACCTTGTACTGGAAA+TGG 0.407592 3.2:+69905483 None:intergenic
GAGATTTAATCAAACGTTTG+TGG 0.448636 3.2:+69905385 None:intergenic
TCAGATTCGGGTAAACCATC+AGG 0.456156 3.2:+69905312 None:intergenic
CTTGGAGCAGAGTTTCTAAA+GGG 0.476848 3.2:-69905363 MS.gene25863:CDS
AGTTTGGCAAGTTGCATGAA+AGG 0.495243 3.2:+69905456 None:intergenic
TCTTGGAGCAGAGTTTCTAA+AGG 0.495785 3.2:-69905364 MS.gene25863:CDS
GCCAAACTCTTTCGTTGCAA+AGG 0.497840 3.2:-69905441 MS.gene25863:CDS
TTTGTACCATTTCCAGTACA+AGG 0.498943 3.2:-69905489 MS.gene25863:CDS
CAGTTTGAGACTATTCCTGA+TGG 0.505144 3.2:-69905327 MS.gene25863:CDS
GTTACTTGCATAATTGCTGA+TGG 0.513827 3.2:-69905178 MS.gene25863:CDS
GTTTGGCAAGTTGCATGAAA+GGG 0.520091 3.2:+69905457 None:intergenic
GGGTTCACATGACCTTGTAC+TGG 0.527592 3.2:+69905477 None:intergenic
ATAATTGCTGATGGAACTGC+GGG 0.534443 3.2:-69905169 MS.gene25863:CDS
TGCGGGCTTTGCTGGAAGAG+TGG 0.571532 3.2:-69905152 MS.gene25863:CDS
ACGAGATGAGCTTGTTAACA+GGG 0.590282 3.2:-69905057 MS.gene25863:CDS
CATAATTGCTGATGGAACTG+CGG 0.630778 3.2:-69905170 MS.gene25863:CDS
AATGGTACAAATACAGCATG+AGG 0.637882 3.2:+69905501 None:intergenic
GAAGAGTGGCAAAAGATTTG+GGG 0.639147 3.2:-69905138 MS.gene25863:CDS
GTTGAATTCAGTGTTCACAA+AGG 0.652579 3.2:+69905407 None:intergenic
CGAGATGAGCTTGTTAACAG+GGG 0.695031 3.2:-69905056 MS.gene25863:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAACGTTTGATTAAATCTCT+TGG - chr3.2:69905201-69905220 MS.gene25863:intron 25.0%
!! TGTTGCATTTTTATCAGATT+CGG + chr3.2:69905286-69905305 None:intergenic 25.0%
!!! TTTTTCACAAGCTTTTTCAA+AGG + chr3.2:69905356-69905375 None:intergenic 25.0%
ATAGTCTCAAACTGAAAATC+TGG + chr3.2:69905249-69905268 None:intergenic 30.0%
GAGATTTAATCAAACGTTTG+TGG + chr3.2:69905200-69905219 None:intergenic 30.0%
! GTTGCATTTTTATCAGATTC+GGG + chr3.2:69905285-69905304 None:intergenic 30.0%
AATGGTACAAATACAGCATG+AGG + chr3.2:69905084-69905103 None:intergenic 35.0%
GTTACTTGCATAATTGCTGA+TGG - chr3.2:69905404-69905423 MS.gene25863:CDS 35.0%
GTTGAATTCAGTGTTCACAA+AGG + chr3.2:69905178-69905197 None:intergenic 35.0%
TATCGTTATGTGAAGCAAGT+AGG - chr3.2:69905316-69905335 MS.gene25863:CDS 35.0%
TCAGCAATTATGCAAGTAAC+TGG + chr3.2:69905404-69905423 None:intergenic 35.0%
TTTGTACCATTTCCAGTACA+AGG - chr3.2:69905093-69905112 MS.gene25863:CDS 35.0%
! GGATTCAAGAACTAGTGTTT+TGG - chr3.2:69905465-69905484 MS.gene25863:CDS 35.0%
ACATGACCTTGTACTGGAAA+TGG + chr3.2:69905102-69905121 None:intergenic 40.0%
ACCTTTGCAACGAAAGAGTT+TGG + chr3.2:69905145-69905164 None:intergenic 40.0%
AGTTTGGCAAGTTGCATGAA+AGG + chr3.2:69905129-69905148 None:intergenic 40.0%
ATAATTGCTGATGGAACTGC+GGG - chr3.2:69905413-69905432 MS.gene25863:CDS 40.0%
CATAATTGCTGATGGAACTG+CGG - chr3.2:69905412-69905431 MS.gene25863:CDS 40.0%
CTTGGAGCAGAGTTTCTAAA+GGG - chr3.2:69905219-69905238 MS.gene25863:intron 40.0%
GAAGAGTGGCAAAAGATTTG+GGG - chr3.2:69905444-69905463 MS.gene25863:CDS 40.0%
GGAAGAGTGGCAAAAGATTT+GGG - chr3.2:69905443-69905462 MS.gene25863:CDS 40.0%
GTTTGGCAAGTTGCATGAAA+GGG + chr3.2:69905128-69905147 None:intergenic 40.0%
TCTTGGAGCAGAGTTTCTAA+AGG - chr3.2:69905218-69905237 MS.gene25863:intron 40.0%
TGGAAGAGTGGCAAAAGATT+TGG - chr3.2:69905442-69905461 MS.gene25863:CDS 40.0%
! ATACAGCATGAGGCTTTTGT+TGG + chr3.2:69905074-69905093 None:intergenic 40.0%
! CAGTTTGAGACTATTCCTGA+TGG - chr3.2:69905255-69905274 MS.gene25863:intron 40.0%
! TACAGCATGAGGCTTTTGTT+GGG + chr3.2:69905073-69905092 None:intergenic 40.0%
!! ACGAGATGAGCTTGTTAACA+GGG - chr3.2:69905525-69905544 MS.gene25863:CDS 40.0%
!! TACGAGATGAGCTTGTTAAC+AGG - chr3.2:69905524-69905543 MS.gene25863:CDS 40.0%
GCCAAACTCTTTCGTTGCAA+AGG - chr3.2:69905141-69905160 MS.gene25863:CDS 45.0%
TCAGATTCGGGTAAACCATC+AGG + chr3.2:69905273-69905292 None:intergenic 45.0%
! TGAGGCTTTTGTTGGGTATG+CGG + chr3.2:69905066-69905085 None:intergenic 45.0%
! TGCTTCTGCTTGTGGTTTGT+TGG - chr3.2:69905490-69905509 MS.gene25863:CDS 45.0%
! TTTTGGACTGCTTCTGCTTG+TGG - chr3.2:69905482-69905501 MS.gene25863:CDS 45.0%
!! CGAGATGAGCTTGTTAACAG+GGG - chr3.2:69905526-69905545 MS.gene25863:CDS 45.0%
!! GCTTCTGCTTGTGGTTTGTT+GGG - chr3.2:69905491-69905510 MS.gene25863:CDS 45.0%
GGGTTCACATGACCTTGTAC+TGG + chr3.2:69905108-69905127 None:intergenic 50.0%
! CTTTTGTTGGGTATGCGGAG+AGG + chr3.2:69905061-69905080 None:intergenic 50.0%
GATGGAACTGCGGGCTTTGC+TGG - chr3.2:69905422-69905441 MS.gene25863:CDS 60.0%
TGCGGGCTTTGCTGGAAGAG+TGG - chr3.2:69905430-69905449 MS.gene25863:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 69905042 69905562 69905042 ID=MS.gene25863
chr3.2 mRNA 69905042 69905562 69905042 ID=MS.gene25863.t1;Parent=MS.gene25863
chr3.2 exon 69905271 69905562 69905271 ID=MS.gene25863.t1.exon1;Parent=MS.gene25863.t1
chr3.2 CDS 69905271 69905562 69905271 ID=cds.MS.gene25863.t1;Parent=MS.gene25863.t1
chr3.2 exon 69905042 69905199 69905042 ID=MS.gene25863.t1.exon2;Parent=MS.gene25863.t1
chr3.2 CDS 69905042 69905199 69905042 ID=cds.MS.gene25863.t1;Parent=MS.gene25863.t1
Gene Sequence

>MS.gene25863

ATGGACTCTTATTCTACCTCTCCGCATACCCAACAAAAGCCTCATGCTGTATTTGTACCATTTCCAGTACAAGGTCATGTGAACCCTTTCATGCAACTTGCCAAACTCTTTCGTTGCAAAGGTTTTCACGTAACCTTTGTGAACACTGAATTCAACCACAAACGTTTGATTAAATCTCTTGGAGCAGAGTTTCTAAAGGGTCTGCCAGATTTTCAGTTTGAGACTATTCCTGATGGTTTACCCGAATCTGATAAAAATGCAACACAAGATGTTGTATCGTTATGTGAAGCAAGTAGGAAAAACTTTTTGCTCCTTTGAAAAAGCTTGTGAAAAAGCTCAACACTTCTTCACTACATATTCCAGTTACTTGCATAATTGCTGATGGAACTGCGGGCTTTGCTGGAAGAGTGGCAAAAGATTTGGGGATTCAAGAACTAGTGTTTTGGACTGCTTCTGCTTGTGGTTTGTTGGGATATTTACAATACGAGATGAGCTTGTTAACAGGGGAATTATTCCCTTGA

Protein sequence

>MS.gene25863.t1

MDSYSTSPHTQQKPHAVFVPFPVQGHVNPFMQLAKLFRCKGFHVTFVNTEFNHKRLIKSLGAEFLKGLPDFQFETIPDGLPESDKNATQDVVSLCEAITCIIADGTAGFAGRVAKDLGIQELVFWTASACGLLGYLQYEMSLLTGELFP