Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26195.t1 | XP_017227796.1 | 50.5 | 214 | 98 | 3 | 1 | 212 | 1 | 208 | 1.20E-51 | 213.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26195.t1 | A0A1U8KII2 | 49.5 | 210 | 100 | 2 | 1 | 209 | 1 | 205 | 2.7e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052311 | MS.gene26195 | 0.806831 | 6.79E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26195.t1 | MTR_7g062720 | 40.541 | 148 | 80 | 3 | 1 | 146 | 1 | 142 | 2.36e-33 | 118 |
| MS.gene26195.t1 | MTR_5g061150 | 33.333 | 111 | 74 | 0 | 40 | 150 | 26 | 136 | 9.82e-19 | 80.1 |
| MS.gene26195.t1 | MTR_4g112730 | 56.338 | 71 | 30 | 1 | 143 | 213 | 2 | 71 | 1.83e-18 | 77.8 |
| MS.gene26195.t1 | MTR_4g037355 | 48.193 | 83 | 32 | 1 | 155 | 237 | 1 | 72 | 1.94e-18 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATTATTGCCCTCATTAA+TGG | 0.192737 | 5.4:-57820791 | MS.gene26195:CDS |
| TGGATCAATGGAACAAATTA+AGG | 0.196151 | 5.4:-57820771 | MS.gene26195:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 5.4:+57820475 | None:intergenic |
| CAAAAGATCCCAAGATCTTA+TGG | 0.237847 | 5.4:-57820849 | MS.gene26195:CDS |
| GACAAAATTAAAGCAAGATT+TGG | 0.252197 | 5.4:-57820826 | MS.gene26195:CDS |
| GATTCTTGAGGGAGACGATT+TGG | 0.271296 | 5.4:-57820968 | MS.gene26195:CDS |
| CTCAATTAAGTTGTTGTTCC+AGG | 0.274617 | 5.4:+57821017 | None:intergenic |
| ATTTACTGAATACTCTGATT+TGG | 0.325598 | 5.4:-57820581 | MS.gene26195:CDS |
| ATTCTTGAGGGAGACGATTT+GGG | 0.354692 | 5.4:-57820967 | MS.gene26195:CDS |
| CGTAGTGGCTATGCTCGTTT+TGG | 0.359061 | 5.4:-57820415 | MS.gene26195:CDS |
| CATAGCCACTACGTCCTCTT+TGG | 0.391081 | 5.4:+57820425 | None:intergenic |
| ACAAAATTAAAGCAAGATTT+GGG | 0.407053 | 5.4:-57820825 | MS.gene26195:CDS |
| TGCCCTCATTAATGGATCAA+TGG | 0.432677 | 5.4:-57820783 | MS.gene26195:CDS |
| TATAACTGAAGTGGAAAAGT+TGG | 0.441180 | 5.4:-57820668 | MS.gene26195:CDS |
| AAAAGATCCCAAGATCTTAT+GGG | 0.458748 | 5.4:-57820848 | MS.gene26195:CDS |
| AACCACCAGTTGCGTCCTAC+AGG | 0.478404 | 5.4:-57820511 | MS.gene26195:CDS |
| TTGTTCCTGTAGGACGCAAC+TGG | 0.480977 | 5.4:+57820506 | None:intergenic |
| ATCAAGCACCACATCGATGA+TGG | 0.494611 | 5.4:-57820895 | MS.gene26195:CDS |
| CTGGCGACAACTACATAACC+TGG | 0.502097 | 5.4:-57821035 | MS.gene26195:CDS |
| GTTCCATTGATCCATTAATG+AGG | 0.509644 | 5.4:+57820780 | None:intergenic |
| GGACTAGACAAGATTCTTGA+GGG | 0.521095 | 5.4:-57820979 | MS.gene26195:CDS |
| CGTGGACGAGGTAGAAATCA+TGG | 0.522365 | 5.4:-57820388 | MS.gene26195:CDS |
| TGCAACAACAATATAGAATG+AGG | 0.523271 | 5.4:-57820606 | MS.gene26195:CDS |
| TTCCATTGATCCATTAATGA+GGG | 0.533283 | 5.4:+57820781 | None:intergenic |
| AATTGAGTATCTTGCATGTG+AGG | 0.565013 | 5.4:-57821001 | MS.gene26195:CDS |
| ATTGAGTATCTTGCATGTGA+GGG | 0.575966 | 5.4:-57821000 | MS.gene26195:CDS |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 5.4:+57820496 | None:intergenic |
| TTTGGTTGCAGCTCTTCTAG+TGG | 0.579996 | 5.4:-57820563 | MS.gene26195:CDS |
| TTCCTGTAGGACGCAACTGG+TGG | 0.593030 | 5.4:+57820509 | None:intergenic |
| GCAACAACAATATAGAATGA+GGG | 0.604762 | 5.4:-57820605 | MS.gene26195:CDS |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 5.4:+57820462 | None:intergenic |
| GGGACTAGACAAGATTCTTG+AGG | 0.613207 | 5.4:-57820980 | MS.gene26195:CDS |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 5.4:-57820439 | MS.gene26195:CDS |
| GTTGCGTCCTACAGGAACAA+TGG | 0.661047 | 5.4:-57820503 | MS.gene26195:CDS |
| AATCGCCAAAGAGGACGTAG+TGG | 0.674563 | 5.4:-57820430 | MS.gene26195:CDS |
| TTTGTAATCCATCATCGATG+TGG | 0.703544 | 5.4:+57820887 | None:intergenic |
| CGATTTGGGAGATGTCCCAG+AGG | 0.725435 | 5.4:-57820953 | MS.gene26195:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATTAAAGCAAGATTT+GGG | - | chr5.4:57820634-57820653 | MS.gene26195:CDS | 20.0% |
| !!! | TTCAAAATTTTAAGCATAAC+TGG | - | chr5.4:57820405-57820424 | MS.gene26195:CDS | 20.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | - | chr5.4:57820878-57820897 | MS.gene26195:CDS | 25.0% |
| ! | GACAAAATTAAAGCAAGATT+TGG | - | chr5.4:57820633-57820652 | MS.gene26195:CDS | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | - | chr5.4:57820762-57820781 | MS.gene26195:CDS | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | + | chr5.4:57820716-57820735 | None:intergenic | 25.0% |
| AAAAGATCCCAAGATCTTAT+GGG | - | chr5.4:57820611-57820630 | MS.gene26195:CDS | 30.0% | |
| AGTATTATTGCCCTCATTAA+TGG | - | chr5.4:57820668-57820687 | MS.gene26195:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | - | chr5.4:57820854-57820873 | MS.gene26195:CDS | 30.0% | |
| TATAACTGAAGTGGAAAAGT+TGG | - | chr5.4:57820791-57820810 | MS.gene26195:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | - | chr5.4:57820853-57820872 | MS.gene26195:CDS | 30.0% | |
| TGGAAAAACTATAACTGAAG+TGG | - | chr5.4:57820782-57820801 | MS.gene26195:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | - | chr5.4:57820688-57820707 | MS.gene26195:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | + | chr5.4:57820681-57820700 | None:intergenic | 30.0% | |
| ! | AATTTTGTCCCATAAGATCT+TGG | + | chr5.4:57820622-57820641 | None:intergenic | 30.0% |
| ! | ATTTTGTCCCATAAGATCTT+GGG | + | chr5.4:57820621-57820640 | None:intergenic | 30.0% |
| ! | TTTTCTTTGCTAATTCCTCT+GGG | + | chr5.4:57820524-57820543 | None:intergenic | 30.0% |
| ! | TTTTTCTTTGCTAATTCCTC+TGG | + | chr5.4:57820525-57820544 | None:intergenic | 30.0% |
| AAAGTTGTGGCGTTTATTTC+AGG | + | chr5.4:57820987-57821006 | None:intergenic | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | - | chr5.4:57820458-57820477 | MS.gene26195:CDS | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | - | chr5.4:57820459-57820478 | MS.gene26195:CDS | 35.0% | |
| CAAAAGATCCCAAGATCTTA+TGG | - | chr5.4:57820610-57820629 | MS.gene26195:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | + | chr5.4:57820682-57820701 | None:intergenic | 35.0% | |
| TTTGTAATCCATCATCGATG+TGG | + | chr5.4:57820575-57820594 | None:intergenic | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | - | chr5.4:57820993-57821012 | MS.gene26195:CDS | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | - | chr5.4:57820994-57821013 | MS.gene26195:CDS | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | + | chr5.4:57820445-57820464 | None:intergenic | 35.0% |
| ACGCCCTTGATTAAAAGTTG+TGG | + | chr5.4:57821000-57821019 | None:intergenic | 40.0% | |
| ATTCTTGAGGGAGACGATTT+GGG | - | chr5.4:57820492-57820511 | MS.gene26195:CDS | 40.0% | |
| GGACTAGACAAGATTCTTGA+GGG | - | chr5.4:57820480-57820499 | MS.gene26195:CDS | 40.0% | |
| ! | AACTTTTCATGCATCTCAGG+TGG | - | chr5.4:57820827-57820846 | MS.gene26195:CDS | 40.0% |
| ! | ACAACTTTTAATCAAGGGCG+TGG | - | chr5.4:57820999-57821018 | MS.gene26195:CDS | 40.0% |
| ! | CTCAACTTTTCATGCATCTC+AGG | - | chr5.4:57820824-57820843 | MS.gene26195:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | - | chr5.4:57820676-57820695 | MS.gene26195:CDS | 40.0% |
| ATCAAGCACCACATCGATGA+TGG | - | chr5.4:57820564-57820583 | MS.gene26195:CDS | 45.0% | |
| GATTCTTGAGGGAGACGATT+TGG | - | chr5.4:57820491-57820510 | MS.gene26195:CDS | 45.0% | |
| GGATATGCCATTGTTCCTGT+AGG | + | chr5.4:57820966-57820985 | None:intergenic | 45.0% | |
| GGGACTAGACAAGATTCTTG+AGG | - | chr5.4:57820479-57820498 | MS.gene26195:CDS | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | - | chr5.4:57821020-57821039 | MS.gene26195:CDS | 45.0% | |
| TTTGGTTGCAGCTCTTCTAG+TGG | - | chr5.4:57820896-57820915 | MS.gene26195:CDS | 45.0% | |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | - | chr5.4:57821002-57821021 | MS.gene26195:CDS | 45.0% |
| AATCGCCAAAGAGGACGTAG+TGG | - | chr5.4:57821029-57821048 | MS.gene26195:CDS | 50.0% | |
| CATAGCCACTACGTCCTCTT+TGG | + | chr5.4:57821037-57821056 | None:intergenic | 50.0% | |
| CGTGGACGAGGTAGAAATCA+TGG | - | chr5.4:57821071-57821090 | MS.gene26195:CDS | 50.0% | |
| CTGGCGACAACTACATAACC+TGG | - | chr5.4:57820424-57820443 | MS.gene26195:CDS | 50.0% | |
| GTTGCGTCCTACAGGAACAA+TGG | - | chr5.4:57820956-57820975 | MS.gene26195:CDS | 50.0% | |
| TTGTTCCTGTAGGACGCAAC+TGG | + | chr5.4:57820956-57820975 | None:intergenic | 50.0% | |
| ! | AGTGGCTATGCTCGTTTTGG+TGG | - | chr5.4:57821047-57821066 | MS.gene26195:CDS | 50.0% |
| ! | CGTAGTGGCTATGCTCGTTT+TGG | - | chr5.4:57821044-57821063 | MS.gene26195:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | - | chr5.4:57821053-57821072 | MS.gene26195:CDS | 50.0% |
| AACCACCAGTTGCGTCCTAC+AGG | - | chr5.4:57820948-57820967 | MS.gene26195:CDS | 55.0% | |
| TTCCTGTAGGACGCAACTGG+TGG | + | chr5.4:57820953-57820972 | None:intergenic | 55.0% | |
| ! | CGATTTGGGAGATGTCCCAG+AGG | - | chr5.4:57820506-57820525 | MS.gene26195:CDS | 55.0% |
| !! | CGTTTTGGTGGTCGTGGACG+AGG | - | chr5.4:57821059-57821078 | MS.gene26195:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 57820384 | 57821097 | 57820384 | ID=MS.gene26195 |
| chr5.4 | mRNA | 57820384 | 57821097 | 57820384 | ID=MS.gene26195.t1;Parent=MS.gene26195 |
| chr5.4 | exon | 57820384 | 57821097 | 57820384 | ID=MS.gene26195.t1.exon1;Parent=MS.gene26195.t1 |
| chr5.4 | CDS | 57820384 | 57821097 | 57820384 | ID=cds.MS.gene26195.t1;Parent=MS.gene26195.t1 |
| Gene Sequence |
| Protein sequence |
