AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26221


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26221.t1 MTR_5g076170 95.057 263 8 1 1 258 1 263 0.0 500
MS.gene26221.t1 MTR_3g063310 62.791 258 89 3 1 258 2 252 1.49e-100 293
MS.gene26221.t1 MTR_1g077560 48.846 260 121 7 1 258 1 250 3.06e-69 214
MS.gene26221.t1 MTR_5g087760 38.424 203 118 3 59 258 56 254 3.08e-43 147
MS.gene26221.t1 MTR_3g078800 37.566 189 109 4 74 258 69 252 5.05e-35 126
MS.gene26221.t1 MTR_2g012550 35.751 193 119 3 69 258 74 264 1.21e-32 120
MS.gene26221.t1 MTR_2g011350 32.515 163 103 3 86 245 35 193 4.42e-22 90.9
MS.gene26221.t1 MTR_3g078820 33.113 151 94 3 93 240 44 190 6.25e-21 88.2
MS.gene26221.t1 MTR_3g074150 26.840 231 146 8 19 243 19 232 3.26e-19 84.3
MS.gene26221.t1 MTR_2g100060 34.545 165 104 3 70 232 60 222 9.03e-19 83.2
MS.gene26221.t1 MTR_3g078810 37.795 127 76 3 121 245 9 134 1.34e-18 80.1
MS.gene26221.t1 MTR_7g106000 31.250 192 118 6 54 236 20 206 3.03e-18 81.3
MS.gene26221.t1 MTR_2g090225 30.435 161 109 3 99 258 62 220 6.83e-18 80.1
MS.gene26221.t1 MTR_3g104460 27.363 201 133 5 67 258 57 253 1.23e-17 80.1
MS.gene26221.t1 MTR_1g057400 29.412 187 123 5 57 236 3 187 2.33e-17 78.6
MS.gene26221.t1 MTR_6g071410 31.847 157 95 4 68 217 2 153 3.37e-17 78.2
MS.gene26221.t1 MTR_6g070890 31.847 157 95 4 68 217 2 153 3.37e-17 78.2
MS.gene26221.t1 MTR_6g070900 34.395 157 91 7 68 217 2 153 4.05e-17 77.8
MS.gene26221.t1 MTR_6g071400 34.395 157 91 7 68 217 2 153 4.05e-17 77.8
MS.gene26221.t1 MTR_1g057270 27.746 173 115 5 92 257 74 243 1.55e-16 77.0
MS.gene26221.t1 MTR_7g105970 26.943 193 134 4 65 252 32 222 3.03e-16 75.9
MS.gene26221.t1 MTR_4g119428 32.979 188 114 7 65 244 41 224 6.18e-16 75.5
MS.gene26221.t1 MTR_2g086790 25.641 195 132 2 75 256 19 213 8.11e-16 74.7
MS.gene26221.t1 MTR_7g106050 29.379 177 124 1 76 252 22 197 1.18e-15 73.9
MS.gene26221.t1 MTR_6g471090 32.450 151 91 5 68 217 11 151 8.39e-15 71.6
MS.gene26221.t1 MTR_7g106120 33.065 124 76 3 61 178 4 126 7.39e-14 68.9
MS.gene26221.t1 MTR_8g016370 28.571 196 110 5 42 232 25 195 7.65e-14 69.3
MS.gene26221.t1 MTR_4g051880 30.769 169 106 4 98 256 44 211 8.42e-14 68.9
MS.gene26221.t1 MTR_7g106010 29.767 215 129 9 57 256 20 227 8.58e-14 68.9
MS.gene26221.t1 MTR_7g106030 28.571 203 134 7 50 244 16 215 4.11e-13 67.4
MS.gene26221.t1 MTR_8g090320 25.888 197 123 6 64 255 79 257 1.83e-11 63.2
MS.gene26221.t1 MTR_7g105870 32.335 167 110 3 72 236 40 205 6.18e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26221.t1 AT1G65690 52.453 265 106 3 1 258 1 252 2.00e-91 270
MS.gene26221.t1 AT5G36970 52.490 261 108 5 1 258 1 248 3.93e-88 262
MS.gene26221.t1 AT1G54540 45.349 258 122 3 1 258 1 239 1.17e-63 199
MS.gene26221.t1 AT2G27080 37.500 192 116 3 62 252 65 253 4.14e-40 139
MS.gene26221.t1 AT2G27080 37.500 192 116 3 62 252 65 253 4.14e-40 139
MS.gene26221.t1 AT5G21130 28.649 185 132 0 62 246 86 270 1.37e-26 104
MS.gene26221.t1 AT3G11650 32.418 182 119 3 65 244 45 224 5.01e-24 97.1
MS.gene26221.t1 AT5G06320 34.940 166 101 5 75 236 48 210 2.42e-23 95.1
MS.gene26221.t1 AT2G35980 35.260 173 103 5 65 232 31 199 5.06e-23 94.4
MS.gene26221.t1 AT2G35460 30.723 166 108 4 75 236 54 216 3.33e-20 87.0
MS.gene26221.t1 AT5G22870 28.333 180 126 2 73 252 27 203 1.96e-17 78.6
MS.gene26221.t1 AT3G52470 29.609 179 123 3 77 254 22 198 1.99e-17 78.6
MS.gene26221.t1 AT4G09590 30.319 188 119 4 73 254 20 201 2.29e-16 75.9
MS.gene26221.t1 AT3G11660 26.087 184 134 1 75 256 18 201 4.11e-16 75.1
MS.gene26221.t1 AT2G35970 30.319 188 119 4 73 254 20 201 1.38e-15 73.9
MS.gene26221.t1 AT2G35960 29.070 172 110 4 89 254 35 200 4.53e-15 72.4
MS.gene26221.t1 AT2G22180 27.094 203 123 5 60 257 108 290 2.12e-14 71.6
MS.gene26221.t1 AT1G17620 25.521 192 119 4 59 232 43 228 1.51e-13 68.9
MS.gene26221.t1 AT3G44220 30.769 156 100 3 84 232 23 177 4.02e-13 67.0
MS.gene26221.t1 AT5G53730 27.389 157 106 3 85 236 37 190 2.03e-12 65.1
MS.gene26221.t1 AT5G06330 28.082 146 103 1 94 237 39 184 3.24e-12 64.3
MS.gene26221.t1 AT4G05220 25.238 210 128 8 48 236 5 206 9.10e-12 63.5

Find 44 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAACCCTGAAATTAATTT+TGG 0.050421 5.4:+58248184 None:intergenic
AGTTCATCGACCGAGTATTT+TGG 0.182422 5.4:+58248551 None:intergenic
CTAATAATTCTATTAGAATT+AGG 0.234955 5.4:-58248133 MS.gene26221:CDS
GGAGCAGTAGTTGTAAATTT+AGG 0.237234 5.4:-58248112 MS.gene26221:CDS
TTGTGACCTTGATAGAATTT+AGG 0.240879 5.4:+58248365 None:intergenic
AGAAATCCAAACAAGAAAAT+TGG 0.264628 5.4:-58248458 MS.gene26221:CDS
AGCTTCCCAAAATTAATTTC+AGG 0.289656 5.4:-58248190 MS.gene26221:CDS
GGAGGACCATGTTGTTGTTC+AGG 0.329559 5.4:+58248752 None:intergenic
CCAAAATTAATTTCAGGGTT+AGG 0.341912 5.4:-58248184 MS.gene26221:CDS
TTTGATTGATTAATAGGAAT+AGG 0.373477 5.4:+58248701 None:intergenic
GGCAAAGTTTGTTGTGTACT+AGG 0.373858 5.4:+58248722 None:intergenic
TGTTTCACCTTCAATTTAAG+AGG 0.405248 5.4:+58248242 None:intergenic
GACATGTTTCTTGGTACTAA+AGG 0.406371 5.4:+58248788 None:intergenic
GTAGTTGTAAATTTAGGTTT+AGG 0.411847 5.4:-58248106 MS.gene26221:CDS
GTTGCTGCAAATTCTTCTGT+TGG 0.423745 5.4:-58248655 MS.gene26221:CDS
ATAGCACTTGGCATAACAAT+TGG 0.433295 5.4:-58248605 MS.gene26221:CDS
GTTCTTAGTATACCTTTGAC+AGG 0.440681 5.4:-58248332 MS.gene26221:CDS
GTTTGTTGTGTACTAGGAAA+AGG 0.446325 5.4:+58248728 None:intergenic
GCTTCCCAAAATTAATTTCA+GGG 0.450968 5.4:-58248189 MS.gene26221:CDS
TCTTTCAAACAACAACAGTT+TGG 0.462291 5.4:-58248510 MS.gene26221:CDS
TAGTGTTCCTCTTAAATTGA+AGG 0.472711 5.4:-58248249 MS.gene26221:CDS
GGACAAACACAGAATGCAAC+AGG 0.474658 5.4:-58248311 MS.gene26221:CDS
CAGGGTTAGGTGTACAATTG+TGG 0.500348 5.4:-58248171 MS.gene26221:CDS
AAAATTGGAATTGACTATAG+AGG 0.508164 5.4:-58248443 MS.gene26221:CDS
TTCTGTGTTTGTCCTGTCAA+AGG 0.510330 5.4:+58248320 None:intergenic
AGCCTGTGAGGATTAAGCTT+GGG 0.514646 5.4:-58248220 MS.gene26221:CDS
CTTGGTACTAAAGGTGCTGA+AGG 0.518344 5.4:+58248797 None:intergenic
TTCCCAAGCTTAATCCTCAC+AGG 0.520897 5.4:+58248218 None:intergenic
TGTTGTGTACTAGGAAAAGG+AGG 0.521253 5.4:+58248731 None:intergenic
TCATTACCTAAATTCTATCA+AGG 0.551476 5.4:-58248371 MS.gene26221:CDS
CAGCCTGTGAGGATTAAGCT+TGG 0.560227 5.4:-58248221 MS.gene26221:CDS
GTGGAAGTCACATAAGTGCT+TGG 0.572955 5.4:-58248421 MS.gene26221:CDS
CAGACACAAAATTATGTGAA+GGG 0.588380 5.4:-58248394 MS.gene26221:CDS
ACCAAAGATTCCAAAATACT+CGG 0.589951 5.4:-58248561 MS.gene26221:CDS
ATTGGAATTGACTATAGAGG+TGG 0.594568 5.4:-58248440 MS.gene26221:CDS
ACAGACACAAAATTATGTGA+AGG 0.595482 5.4:-58248395 MS.gene26221:CDS
GGTGCTGAAGGTTGTTGTTG+AGG 0.603656 5.4:+58248809 None:intergenic
CAGCAACAAGTTCAGTCAGA+GGG 0.607080 5.4:-58248272 MS.gene26221:CDS
GGTTAGGTGTACAATTGTGG+TGG 0.630853 5.4:-58248168 MS.gene26221:CDS
TGAAGGTGAAACAGCCTGTG+AGG 0.645437 5.4:-58248232 MS.gene26221:CDS
GCAGCAACAAGTTCAGTCAG+AGG 0.650897 5.4:-58248273 MS.gene26221:CDS
GGTGCTTCAACATCATGAAG+TGG 0.676498 5.4:+58248830 None:intergenic
TGTGTACTAGGAAAAGGAGG+AGG 0.681120 5.4:+58248734 None:intergenic
GATGATCCTGAACAACAACA+TGG 0.735600 5.4:-58248758 MS.gene26221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATAATTCTATTAGAATT+AGG - chr5.4:58248821-58248840 MS.gene26221:CDS 15.0%
!! TTTGATTGATTAATAGGAAT+AGG + chr5.4:58248256-58248275 None:intergenic 20.0%
! AAAATTGGAATTGACTATAG+AGG - chr5.4:58248511-58248530 MS.gene26221:CDS 25.0%
! AGAAATCCAAACAAGAAAAT+TGG - chr5.4:58248496-58248515 MS.gene26221:CDS 25.0%
! TCATTACCTAAATTCTATCA+AGG - chr5.4:58248583-58248602 MS.gene26221:CDS 25.0%
!! CTAACCCTGAAATTAATTTT+GGG + chr5.4:58248772-58248791 None:intergenic 25.0%
!! GTAGTTGTAAATTTAGGTTT+AGG - chr5.4:58248848-58248867 MS.gene26221:CDS 25.0%
!! TCAATTCCAATTTTCTTGTT+TGG + chr5.4:58248505-58248524 None:intergenic 25.0%
!!! ATATTTTTGATCATAGCACT+TGG - chr5.4:58248337-58248356 MS.gene26221:CDS 25.0%
ACAGACACAAAATTATGTGA+AGG - chr5.4:58248559-58248578 MS.gene26221:CDS 30.0%
ACCAAAGATTCCAAAATACT+CGG - chr5.4:58248393-58248412 MS.gene26221:CDS 30.0%
AGCTTCCCAAAATTAATTTC+AGG - chr5.4:58248764-58248783 MS.gene26221:CDS 30.0%
CAGACACAAAATTATGTGAA+GGG - chr5.4:58248560-58248579 MS.gene26221:CDS 30.0%
CCAAAATTAATTTCAGGGTT+AGG - chr5.4:58248770-58248789 MS.gene26221:CDS 30.0%
GAATCTTTGGTCTAAAAACA+AGG + chr5.4:58248384-58248403 None:intergenic 30.0%
GCTTCCCAAAATTAATTTCA+GGG - chr5.4:58248765-58248784 MS.gene26221:CDS 30.0%
TAGTGTTCCTCTTAAATTGA+AGG - chr5.4:58248705-58248724 MS.gene26221:CDS 30.0%
TCTTTCAAACAACAACAGTT+TGG - chr5.4:58248444-58248463 MS.gene26221:CDS 30.0%
TGTTTCACCTTCAATTTAAG+AGG + chr5.4:58248715-58248734 None:intergenic 30.0%
TTGTGACCTTGATAGAATTT+AGG + chr5.4:58248592-58248611 None:intergenic 30.0%
! CCTAACCCTGAAATTAATTT+TGG + chr5.4:58248773-58248792 None:intergenic 30.0%
!! TCAGATTTTGACATGTTTCT+TGG + chr5.4:58248178-58248197 None:intergenic 30.0%
!!! GGTGGTTTTGATTGATTAAT+AGG + chr5.4:58248262-58248281 None:intergenic 30.0%
ATAGCACTTGGCATAACAAT+TGG - chr5.4:58248349-58248368 MS.gene26221:CDS 35.0%
ATTGGAATTGACTATAGAGG+TGG - chr5.4:58248514-58248533 MS.gene26221:CDS 35.0%
GACATGTTTCTTGGTACTAA+AGG + chr5.4:58248169-58248188 None:intergenic 35.0%
GGAGCAGTAGTTGTAAATTT+AGG - chr5.4:58248842-58248861 MS.gene26221:CDS 35.0%
GTTCTTAGTATACCTTTGAC+AGG - chr5.4:58248622-58248641 MS.gene26221:CDS 35.0%
! CAACAGCTTCTTCTTTTCTT+TGG + chr5.4:58248283-58248302 None:intergenic 35.0%
!! GTTTGTTGTGTACTAGGAAA+AGG + chr5.4:58248229-58248248 None:intergenic 35.0%
!!! ACCGAGTATTTTGGAATCTT+TGG + chr5.4:58248397-58248416 None:intergenic 35.0%
GATGATCCTGAACAACAACA+TGG - chr5.4:58248196-58248215 MS.gene26221:CDS 40.0%
GTTGCTGCAAATTCTTCTGT+TGG - chr5.4:58248299-58248318 MS.gene26221:CDS 40.0%
TGTTGTGTACTAGGAAAAGG+AGG + chr5.4:58248226-58248245 None:intergenic 40.0%
TTCTGTGTTTGTCCTGTCAA+AGG + chr5.4:58248637-58248656 None:intergenic 40.0%
! AGTTCATCGACCGAGTATTT+TGG + chr5.4:58248406-58248425 None:intergenic 40.0%
! CAGCTTCTTCTTTTCTTTGG+TGG + chr5.4:58248280-58248299 None:intergenic 40.0%
!! GGCAAAGTTTGTTGTGTACT+AGG + chr5.4:58248235-58248254 None:intergenic 40.0%
AGCCTGTGAGGATTAAGCTT+GGG - chr5.4:58248734-58248753 MS.gene26221:CDS 45.0%
CAGCAACAAGTTCAGTCAGA+GGG - chr5.4:58248682-58248701 MS.gene26221:CDS 45.0%
CAGGGTTAGGTGTACAATTG+TGG - chr5.4:58248783-58248802 MS.gene26221:CDS 45.0%
GGACAAACACAGAATGCAAC+AGG - chr5.4:58248643-58248662 MS.gene26221:CDS 45.0%
GGTGCTTCAACATCATGAAG+TGG + chr5.4:58248127-58248146 None:intergenic 45.0%
GGTTAGGTGTACAATTGTGG+TGG - chr5.4:58248786-58248805 MS.gene26221:CDS 45.0%
GTGGAAGTCACATAAGTGCT+TGG - chr5.4:58248533-58248552 MS.gene26221:CDS 45.0%
TGTGTACTAGGAAAAGGAGG+AGG + chr5.4:58248223-58248242 None:intergenic 45.0%
TTCCCAAGCTTAATCCTCAC+AGG + chr5.4:58248739-58248758 None:intergenic 45.0%
!! CTTGGTACTAAAGGTGCTGA+AGG + chr5.4:58248160-58248179 None:intergenic 45.0%
CAGCCTGTGAGGATTAAGCT+TGG - chr5.4:58248733-58248752 MS.gene26221:CDS 50.0%
GCAGCAACAAGTTCAGTCAG+AGG - chr5.4:58248681-58248700 MS.gene26221:CDS 50.0%
TGAAGGTGAAACAGCCTGTG+AGG - chr5.4:58248722-58248741 MS.gene26221:CDS 50.0%
! GGAGGACCATGTTGTTGTTC+AGG + chr5.4:58248205-58248224 None:intergenic 50.0%
! GGTGCTGAAGGTTGTTGTTG+AGG + chr5.4:58248148-58248167 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 58248100 58248876 58248100 ID=MS.gene26221
chr5.4 mRNA 58248100 58248876 58248100 ID=MS.gene26221.t1;Parent=MS.gene26221
chr5.4 exon 58248100 58248876 58248100 ID=MS.gene26221.t1.exon1;Parent=MS.gene26221.t1
chr5.4 CDS 58248100 58248876 58248100 ID=cds.MS.gene26221.t1;Parent=MS.gene26221.t1
Gene Sequence

>MS.gene26221

ATGGCAGATAATCAAAGAATTCATCCACTTCATGATGTTGAAGCACCTCAACAACAACCTTCAGCACCTTTAGTACCAAGAAACATGTCAAAATCTGATGATCCTGAACAACAACATGGTCCTCCTCCTTTTCCTAGTACACAACAAACTTTGCCTATTCCTATTAATCAATCAAAACCACCAAAGAAAAGAAGAAGCTGTTGCTGCAAATTCTTCTGTTGGATTTTCAGCATACTAATATTTTTGATCATAGCACTTGGCATAACAATTGGAATACTCTTCCTTGTTTTTAGACCAAAGATTCCAAAATACTCGGTCGATGAACTACGTGTCACGCAATTCGATCTTTCAAACAACAACAGTTTGGCAGTCACATTCAACTTAACAATCACTGCAAGAAATCCAAACAAGAAAATTGGAATTGACTATAGAGGTGGAAGTCACATAAGTGCTTGGTATACAGACACAAAATTATGTGAAGGGTCATTACCTAAATTCTATCAAGGTCACAAAAATGTTACTGTTCTTAGTATACCTTTGACAGGACAAACACAGAATGCAACAGGTTTGAGAGATTCATTGCAGCAACAAGTTCAGTCAGAGGGTAGTGTTCCTCTTAAATTGAAGGTGAAACAGCCTGTGAGGATTAAGCTTGGGAAACTGAAGCTTCCCAAAATTAATTTCAGGGTTAGGTGTACAATTGTGGTGGATAGTCTTAGTGCTAATAATTCTATTAGAATTAGGAGCAGTAGTTGTAAATTTAGGTTTAGGCTTTGA

Protein sequence

>MS.gene26221.t1

MADNQRIHPLHDVEAPQQQPSAPLVPRNMSKSDDPEQQHGPPPFPSTQQTLPIPINQSKPPKKRRSCCCKFFCWIFSILIFLIIALGITIGILFLVFRPKIPKYSVDELRVTQFDLSNNNSLAVTFNLTITARNPNKKIGIDYRGGSHISAWYTDTKLCEGSLPKFYQGHKNVTVLSIPLTGQTQNATGLRDSLQQQVQSEGSVPLKLKVKQPVRIKLGKLKLPKINFRVRCTIVVDSLSANNSIRIRSSSCKFRFRL