Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26496.t1 | XP_013454922.1 | 76.8 | 552 | 95 | 2 | 1 | 552 | 1 | 519 | 1.10E-148 | 537 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26496.t1 | Q7XJK8 | 28.3 | 237 | 156 | 4 | 6 | 233 | 2 | 233 | 1.2e-15 | 86.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26496.t1 | A0A072USW1 | 76.8 | 552 | 95 | 2 | 1 | 552 | 1 | 519 | 7.6e-149 | 537.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene26496.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05456 | MS.gene26496 | 0.812743 | 3.64E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26496.t1 | MTR_4g019670 | 80.143 | 559 | 64 | 3 | 1 | 552 | 1 | 519 | 0.0 | 823 |
| MS.gene26496.t1 | MTR_7g106510 | 79.249 | 559 | 53 | 5 | 1 | 552 | 1 | 503 | 0.0 | 810 |
| MS.gene26496.t1 | MTR_3g093900 | 47.160 | 581 | 267 | 12 | 1 | 552 | 1 | 570 | 6.13e-149 | 440 |
| MS.gene26496.t1 | MTR_8g467500 | 50.896 | 279 | 129 | 2 | 13 | 287 | 13 | 287 | 1.77e-67 | 220 |
| MS.gene26496.t1 | MTR_3g466980 | 32.727 | 165 | 107 | 2 | 1 | 164 | 1 | 162 | 1.04e-23 | 100 |
| MS.gene26496.t1 | MTR_5g075380 | 33.974 | 156 | 101 | 2 | 7 | 161 | 4 | 158 | 7.13e-23 | 95.9 |
| MS.gene26496.t1 | MTR_4g032620 | 36.066 | 122 | 78 | 0 | 6 | 127 | 3 | 124 | 2.61e-21 | 93.2 |
| MS.gene26496.t1 | MTR_8g036130 | 38.168 | 131 | 79 | 1 | 4 | 132 | 1 | 131 | 3.30e-21 | 93.6 |
| MS.gene26496.t1 | MTR_3g466830 | 33.333 | 150 | 99 | 1 | 6 | 154 | 2 | 151 | 9.66e-20 | 86.7 |
| MS.gene26496.t1 | MTR_4g031910 | 41.667 | 132 | 74 | 1 | 1 | 132 | 1 | 129 | 1.94e-19 | 87.8 |
| MS.gene26496.t1 | MTR_2g016210 | 30.345 | 145 | 100 | 1 | 7 | 150 | 4 | 148 | 1.30e-18 | 85.9 |
| MS.gene26496.t1 | MTR_3g065100 | 33.113 | 151 | 100 | 1 | 6 | 155 | 3 | 153 | 9.88e-18 | 83.2 |
| MS.gene26496.t1 | MTR_1g090710 | 32.174 | 115 | 77 | 1 | 6 | 120 | 3 | 116 | 1.40e-17 | 80.5 |
| MS.gene26496.t1 | MTR_3g466890 | 30.065 | 153 | 106 | 1 | 6 | 157 | 2 | 154 | 1.97e-17 | 80.1 |
| MS.gene26496.t1 | MTR_4g032260 | 41.053 | 95 | 56 | 0 | 38 | 132 | 35 | 129 | 2.08e-17 | 80.1 |
| MS.gene26496.t1 | MTR_5g047580 | 34.167 | 120 | 75 | 2 | 1 | 120 | 1 | 116 | 1.97e-16 | 77.0 |
| MS.gene26496.t1 | MTR_4g032290 | 31.613 | 155 | 87 | 3 | 38 | 175 | 35 | 187 | 2.55e-16 | 79.0 |
| MS.gene26496.t1 | MTR_2g035610 | 36.800 | 125 | 76 | 1 | 1 | 125 | 1 | 122 | 2.87e-16 | 75.5 |
| MS.gene26496.t1 | MTR_3g031100 | 29.915 | 117 | 82 | 0 | 6 | 122 | 3 | 119 | 3.50e-16 | 79.0 |
| MS.gene26496.t1 | MTR_3g031240 | 27.350 | 117 | 85 | 0 | 6 | 122 | 3 | 119 | 1.85e-15 | 75.1 |
| MS.gene26496.t1 | MTR_4g028800 | 42.857 | 70 | 38 | 1 | 7 | 76 | 4 | 71 | 2.24e-15 | 71.6 |
| MS.gene26496.t1 | MTR_4g063790 | 28.750 | 160 | 103 | 4 | 1 | 155 | 1 | 154 | 2.62e-15 | 74.3 |
| MS.gene26496.t1 | MTR_1g090783 | 25.517 | 145 | 107 | 1 | 6 | 150 | 2 | 145 | 5.71e-15 | 73.2 |
| MS.gene26496.t1 | MTR_1g090697 | 33.333 | 117 | 73 | 2 | 1 | 115 | 1 | 114 | 7.78e-15 | 72.8 |
| MS.gene26496.t1 | MTR_3g466930 | 33.065 | 124 | 82 | 1 | 35 | 157 | 2 | 125 | 7.81e-15 | 72.0 |
| MS.gene26496.t1 | MTR_1g077390 | 30.328 | 122 | 83 | 2 | 6 | 126 | 3 | 123 | 1.53e-14 | 71.6 |
| MS.gene26496.t1 | MTR_3g466900 | 31.452 | 124 | 84 | 1 | 35 | 157 | 2 | 125 | 2.60e-14 | 70.5 |
| MS.gene26496.t1 | MTR_1g077300 | 32.800 | 125 | 79 | 2 | 6 | 126 | 3 | 126 | 2.71e-13 | 68.2 |
| MS.gene26496.t1 | MTR_4g028720 | 34.694 | 147 | 92 | 3 | 6 | 149 | 3 | 148 | 2.89e-13 | 68.2 |
| MS.gene26496.t1 | MTR_1g084950 | 37.097 | 124 | 70 | 3 | 1 | 120 | 1 | 120 | 3.30e-13 | 67.8 |
| MS.gene26496.t1 | MTR_1g077320 | 34.167 | 120 | 75 | 2 | 1 | 120 | 1 | 116 | 8.41e-13 | 67.0 |
| MS.gene26496.t1 | MTR_7g011950 | 32.237 | 152 | 96 | 4 | 1 | 149 | 1 | 148 | 2.04e-12 | 65.9 |
| MS.gene26496.t1 | MTR_3g467080 | 26.190 | 126 | 89 | 2 | 6 | 127 | 3 | 128 | 8.41e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26496.t1 | AT1G65300 | 29.437 | 231 | 135 | 6 | 6 | 220 | 2 | 220 | 2.11e-20 | 92.0 |
| MS.gene26496.t1 | AT2G40210 | 28.743 | 167 | 116 | 2 | 4 | 170 | 1 | 164 | 8.54e-19 | 89.0 |
| MS.gene26496.t1 | AT3G05860 | 32.576 | 132 | 85 | 2 | 1 | 131 | 1 | 129 | 4.99e-17 | 81.6 |
| MS.gene26496.t1 | AT3G05860 | 32.576 | 132 | 85 | 2 | 1 | 131 | 1 | 129 | 5.09e-17 | 81.3 |
| MS.gene26496.t1 | AT5G26650 | 32.456 | 114 | 77 | 0 | 7 | 120 | 2 | 115 | 1.22e-16 | 82.4 |
| MS.gene26496.t1 | AT3G05860 | 32.576 | 132 | 85 | 2 | 1 | 131 | 1 | 129 | 1.62e-16 | 79.0 |
| MS.gene26496.t1 | AT1G31630 | 33.043 | 115 | 76 | 1 | 6 | 120 | 2 | 115 | 2.56e-16 | 80.9 |
| MS.gene26496.t1 | AT5G27960 | 30.370 | 135 | 86 | 1 | 7 | 133 | 2 | 136 | 3.48e-16 | 80.1 |
| MS.gene26496.t1 | AT5G06500 | 29.861 | 144 | 100 | 1 | 6 | 149 | 3 | 145 | 2.63e-15 | 76.3 |
| MS.gene26496.t1 | AT1G31640 | 31.304 | 115 | 78 | 1 | 6 | 120 | 2 | 115 | 2.64e-15 | 79.0 |
| MS.gene26496.t1 | AT2G28700 | 31.624 | 117 | 80 | 0 | 4 | 120 | 1 | 117 | 5.41e-15 | 76.6 |
| MS.gene26496.t1 | AT1G65330 | 32.743 | 113 | 76 | 0 | 8 | 120 | 4 | 116 | 9.82e-15 | 75.1 |
| MS.gene26496.t1 | AT5G26630 | 33.708 | 178 | 109 | 5 | 1 | 177 | 1 | 170 | 1.17e-14 | 73.9 |
| MS.gene26496.t1 | AT5G48670 | 35.043 | 117 | 76 | 0 | 6 | 122 | 3 | 119 | 1.91e-14 | 75.1 |
| MS.gene26496.t1 | AT2G15660 | 28.846 | 156 | 104 | 5 | 40 | 194 | 30 | 179 | 3.85e-13 | 70.1 |
| MS.gene26496.t1 | AT1G22590 | 26.606 | 109 | 77 | 1 | 6 | 114 | 3 | 108 | 3.86e-13 | 68.2 |
| MS.gene26496.t1 | AT5G27810 | 31.034 | 87 | 60 | 0 | 34 | 120 | 3 | 89 | 5.48e-11 | 60.5 |
Find 118 sgRNAs with CRISPR-Local
Find 145 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGCTAATCAAGCTAATTT+TGG | 0.158650 | 7.3:-7684196 | MS.gene26496:CDS |
| TTGCTTCCTCAAGAAAATTT+TGG | 0.166377 | 7.3:-7683707 | MS.gene26496:CDS |
| AACTTTGGTGGCAATATTAA+TGG | 0.198018 | 7.3:-7683359 | MS.gene26496:CDS |
| TTGCCTCCTCAAGGATATTT+TGG | 0.199048 | 7.3:-7683929 | MS.gene26496:CDS |
| TTGACTCAAACTTGGTTATT+AGG | 0.210241 | 7.3:+7684707 | None:intergenic |
| CAAAATCTTCAAAGTTAAAT+TGG | 0.211371 | 7.3:+7683681 | None:intergenic |
| TTGTCTCATCAAGGAAATTT+TGG | 0.233564 | 7.3:-7683590 | MS.gene26496:CDS |
| GATTGTCACATGCTCCATTT+TGG | 0.244676 | 7.3:+7684816 | None:intergenic |
| CCAACAACCCTTGTCAGATT+TGG | 0.245325 | 7.3:-7684320 | MS.gene26496:CDS |
| GTTTCTTCCTCGAGGAAATC+TGG | 0.271699 | 7.3:-7684026 | MS.gene26496:CDS |
| ACGGTGATGGGGTGCATAAC+GGG | 0.286961 | 7.3:-7684369 | MS.gene26496:CDS |
| GAAAGGGTTGTTCACCAAAA+TGG | 0.296295 | 7.3:-7684830 | MS.gene26496:CDS |
| AGAAAATGAAGAGACTTTAA+TGG | 0.308700 | 7.3:-7684587 | MS.gene26496:CDS |
| AACTTTGTTGACAATAATTA+CGG | 0.325383 | 7.3:-7683311 | MS.gene26496:CDS |
| GAAGTTCAAGAAAACTTTAG+AGG | 0.330050 | 7.3:-7683473 | MS.gene26496:CDS |
| GAGGCATGTGCGGTTGTGTT+TGG | 0.339289 | 7.3:-7684781 | MS.gene26496:CDS |
| CAACAACCCTTGTCAGATTT+GGG | 0.353572 | 7.3:-7684319 | MS.gene26496:CDS |
| CAAGTTTGAGTCAATGTCAT+TGG | 0.377334 | 7.3:-7684698 | MS.gene26496:CDS |
| AGGCATGTGCGGTTGTGTTT+GGG | 0.389221 | 7.3:-7684780 | MS.gene26496:CDS |
| TTTGAAGGTCAAGTAAACTT+TGG | 0.391480 | 7.3:-7683797 | MS.gene26496:CDS |
| GTGATACAAAACCATCAATA+TGG | 0.392745 | 7.3:-7684753 | MS.gene26496:CDS |
| GCAGGAGATGGCCATATTGA+TGG | 0.394229 | 7.3:+7684742 | None:intergenic |
| CTTCCACTTGCAGATCTTAG+TGG | 0.402967 | 7.3:-7684235 | MS.gene26496:CDS |
| AAACCACCACTAGAATATTC+AGG | 0.410956 | 7.3:+7684142 | None:intergenic |
| GAAAATGAAGAGACTTTAAT+GGG | 0.430958 | 7.3:-7684586 | MS.gene26496:CDS |
| AACAATATGGGTAGCAACAT+TGG | 0.431457 | 7.3:-7683992 | MS.gene26496:CDS |
| GATGTCTAGGCATTTAGAAA+AGG | 0.433553 | 7.3:-7684452 | MS.gene26496:CDS |
| ATGTCTAGGCATTTAGAAAA+GGG | 0.436802 | 7.3:-7684451 | MS.gene26496:CDS |
| AACGGTGATGGGGTGCATAA+CGG | 0.439629 | 7.3:-7684370 | MS.gene26496:CDS |
| TGTGCGGTTGTGTTTGGGCC+AGG | 0.446264 | 7.3:-7684775 | MS.gene26496:CDS |
| CAATGACATTGACTCAAACT+TGG | 0.455302 | 7.3:+7684699 | None:intergenic |
| CCAAATCTGACAAGGGTTGT+TGG | 0.456247 | 7.3:+7684320 | None:intergenic |
| TTTCTTCCTCGAGGAAATCT+GGG | 0.457179 | 7.3:-7684025 | MS.gene26496:CDS |
| TTTCATCAAAACAACTTTGG+TGG | 0.457377 | 7.3:-7683371 | MS.gene26496:CDS |
| GGTGGCAATATTAATGGAGA+TGG | 0.459529 | 7.3:-7683353 | MS.gene26496:CDS |
| TTACTTTACACAACGGTGAT+GGG | 0.461878 | 7.3:-7684381 | MS.gene26496:CDS |
| GGAGGTCAAAGCAACTTTCA+AGG | 0.462180 | 7.3:-7683908 | MS.gene26496:CDS |
| CATAACGGGGTTAACATGTA+TGG | 0.462868 | 7.3:-7684355 | MS.gene26496:CDS |
| TTGTGTAAAGTAAAAGATAT+TGG | 0.463560 | 7.3:+7684391 | None:intergenic |
| ATACCACCACATACAAATCT+TGG | 0.466401 | 7.3:-7683632 | MS.gene26496:CDS |
| ACTTTGTTGACAATAATTAC+GGG | 0.467038 | 7.3:-7683310 | MS.gene26496:CDS |
| ATATCAACACCACCATTGCT+TGG | 0.469029 | 7.3:+7683401 | None:intergenic |
| ATATTAATGGAGATGGGATG+TGG | 0.470498 | 7.3:-7683346 | MS.gene26496:CDS |
| TACCACATGCAAATCTGAAT+GGG | 0.471278 | 7.3:-7683850 | MS.gene26496:CDS |
| TTTACTTTACACAACGGTGA+TGG | 0.473941 | 7.3:-7684382 | MS.gene26496:CDS |
| ACAATATGGGTAGCAACATT+GGG | 0.477464 | 7.3:-7683991 | MS.gene26496:CDS |
| CTACCACATGCAAATCTGAA+TGG | 0.479159 | 7.3:-7683851 | MS.gene26496:CDS |
| TACGGGAGCAACTTCAACCC+TGG | 0.503874 | 7.3:-7683293 | MS.gene26496:CDS |
| GATATTGGTGCCAACGGTGG+AGG | 0.504265 | 7.3:+7684406 | None:intergenic |
| CTTGCAGATCTTAGTGGTGG+TGG | 0.505108 | 7.3:-7684229 | MS.gene26496:CDS |
| ATTAGGTCTTCAGCCACTGC+AGG | 0.513329 | 7.3:+7684724 | None:intergenic |
| AACAACCTTGGAAGTAGCAT+GGG | 0.513933 | 7.3:-7684262 | MS.gene26496:CDS |
| ATTGGTGCCAACGGTGGAGG+AGG | 0.517191 | 7.3:+7684409 | None:intergenic |
| ATACCACCTCATACAAATCC+TGG | 0.520709 | 7.3:-7683536 | MS.gene26496:CDS |
| TGAGAATAAAACTCGAAGAA+AGG | 0.533789 | 7.3:-7684872 | MS.gene26496:CDS |
| GACATTACAGGTAGCAACAC+AGG | 0.536241 | 7.3:-7683878 | MS.gene26496:CDS |
| GGTGGAGGAGGATTATTAAG+AGG | 0.544411 | 7.3:+7684421 | None:intergenic |
| GTGGCAATATTAATGGAGAT+GGG | 0.552269 | 7.3:-7683352 | MS.gene26496:CDS |
| CCAACACCAGGATTTGTATG+AGG | 0.556800 | 7.3:+7683530 | None:intergenic |
| TCTTCAGCCACTGCAGGAGA+TGG | 0.560210 | 7.3:+7684730 | None:intergenic |
| TGCTATAAGAGAAGATTGAA+AGG | 0.560605 | 7.3:-7684847 | MS.gene26496:CDS |
| TGGTGGTGGTTGTGATAACT+TGG | 0.567708 | 7.3:-7684215 | MS.gene26496:CDS |
| AGATCACTAGAAAGATGTCT+AGG | 0.570407 | 7.3:-7684465 | MS.gene26496:CDS |
| TAACAACCTTGGAAGTAGCA+TGG | 0.574908 | 7.3:-7684263 | MS.gene26496:CDS |
| TACTTTACACAACGGTGATG+GGG | 0.579959 | 7.3:-7684380 | MS.gene26496:CDS |
| GTAGGTGTGTTGCCTCCTCA+AGG | 0.582180 | 7.3:-7683938 | MS.gene26496:CDS |
| TAATAATCCTCCTCCACCGT+TGG | 0.582273 | 7.3:-7684416 | MS.gene26496:CDS |
| AGTGGTTTCAATAACAACCT+TGG | 0.589310 | 7.3:-7684274 | MS.gene26496:CDS |
| CCACTAGAATATTCAGGTGA+TGG | 0.589851 | 7.3:+7684148 | None:intergenic |
| GTATCCCCATGCTACTTCCA+AGG | 0.590776 | 7.3:+7684257 | None:intergenic |
| CATGATATGTTTCTTCCTCG+AGG | 0.591879 | 7.3:-7684034 | MS.gene26496:CDS |
| GTAAAAGATATTGGTGCCAA+CGG | 0.592046 | 7.3:+7684400 | None:intergenic |
| CGGTGATGGGGTGCATAACG+GGG | 0.594137 | 7.3:-7684368 | MS.gene26496:CDS |
| GACAATCTTATGTGGTATTG+AGG | 0.594613 | 7.3:-7684800 | MS.gene26496:CDS |
| GCTATAAGAGAAGATTGAAA+GGG | 0.594807 | 7.3:-7684846 | MS.gene26496:CDS |
| TTTATTCAGAAGGAAATCCA+GGG | 0.595152 | 7.3:+7683276 | None:intergenic |
| TTTCTCGCCCAAATCTGACA+AGG | 0.596335 | 7.3:+7684312 | None:intergenic |
| CCACATACAAATCTTGGTGT+TGG | 0.598589 | 7.3:-7683626 | MS.gene26496:CDS |
| ATAATATATGTAGCAACATG+TGG | 0.610672 | 7.3:-7683442 | MS.gene26496:CDS |
| CCACTTGCAGATCTTAGTGG+TGG | 0.610771 | 7.3:-7684232 | MS.gene26496:CDS |
| CATGAAAATCCAAGCAATGG+TGG | 0.610991 | 7.3:-7683410 | MS.gene26496:CDS |
| TTCTCGCCCAAATCTGACAA+GGG | 0.612284 | 7.3:+7684313 | None:intergenic |
| CCACATGAAAATCCAAGCAA+TGG | 0.614633 | 7.3:-7683413 | MS.gene26496:CDS |
| TCACCTGAATATTCTAGTGG+TGG | 0.615267 | 7.3:-7684145 | MS.gene26496:CDS |
| AAAGATATTGGTGCCAACGG+TGG | 0.617732 | 7.3:+7684403 | None:intergenic |
| ACAACACATGTAGCGGCATG+TGG | 0.617861 | 7.3:-7683763 | MS.gene26496:CDS |
| CCTCATACAAATCCTGGTGT+TGG | 0.617961 | 7.3:-7683530 | MS.gene26496:CDS |
| AAAGTTGATCAAATGAGCAG+TGG | 0.618596 | 7.3:-7684292 | MS.gene26496:CDS |
| CCATCACATGAAAATCATAG+TGG | 0.621754 | 7.3:-7684064 | MS.gene26496:CDS |
| TGTCTAGGCATTTAGAAAAG+GGG | 0.623976 | 7.3:-7684450 | MS.gene26496:CDS |
| GAAAATCCTAGTGGTGGTGT+AGG | 0.627650 | 7.3:-7683956 | MS.gene26496:CDS |
| CCATCACCTGAATATTCTAG+TGG | 0.635359 | 7.3:-7684148 | MS.gene26496:CDS |
| GTTGATGTGTTGTCTCATCA+AGG | 0.635762 | 7.3:-7683503 | MS.gene26496:CDS |
| GTTGATGTGTTGTCTCATCA+AGG | 0.635762 | 7.3:-7683599 | MS.gene26496:CDS |
| ATGCATCAAATTGAAAACGA+AGG | 0.641960 | 7.3:-7684556 | MS.gene26496:CDS |
| TTTGGAGTTGATAACAACAC+AGG | 0.642445 | 7.3:-7683572 | MS.gene26496:CDS |
| CCACATTCAAATTCGAGTGG+TGG | 0.643895 | 7.3:-7683737 | MS.gene26496:CDS |
| TTCTTCCTCGAGGAAATCTG+GGG | 0.645397 | 7.3:-7684024 | MS.gene26496:CDS |
| ACACCAAGATTTGTATGTGG+TGG | 0.650459 | 7.3:+7683629 | None:intergenic |
| AACACACCTACACCACCACT+AGG | 0.650603 | 7.3:+7683950 | None:intergenic |
| ACATCAACATCACCAACACC+AGG | 0.652418 | 7.3:+7683518 | None:intergenic |
| TTTGATGACAACACATGTAG+CGG | 0.654217 | 7.3:-7683770 | MS.gene26496:CDS |
| AATATGGCCATCTCCTGCAG+TGG | 0.655817 | 7.3:-7684737 | MS.gene26496:CDS |
| CCTCCAAAATATCCTTGAGG+AGG | 0.656666 | 7.3:+7683926 | None:intergenic |
| CCAACACCAAGATTTGTATG+TGG | 0.658592 | 7.3:+7683626 | None:intergenic |
| ATGTGGTATTGAGGCATGTG+CGG | 0.658684 | 7.3:-7684791 | MS.gene26496:CDS |
| TGACCTCCAAAATATCCTTG+AGG | 0.660910 | 7.3:+7683923 | None:intergenic |
| TTCCCATTCAGATTTGCATG+TGG | 0.661550 | 7.3:+7683848 | None:intergenic |
| CCACCACTCGAATTTGAATG+TGG | 0.662521 | 7.3:+7683737 | None:intergenic |
| GAGCATGTGACAATCTTATG+TGG | 0.668241 | 7.3:-7684808 | MS.gene26496:CDS |
| ACACCAGGATTTGTATGAGG+TGG | 0.668736 | 7.3:+7683533 | None:intergenic |
| CCACCACTAAGATCTGCAAG+TGG | 0.669203 | 7.3:+7684232 | None:intergenic |
| ACATTACAGGTAGCAACACA+GGG | 0.671623 | 7.3:-7683877 | MS.gene26496:CDS |
| TCACATGAAAATCCTAGTGG+TGG | 0.674534 | 7.3:-7683962 | MS.gene26496:CDS |
| CAATCACATGAAAATCCTAG+TGG | 0.684612 | 7.3:-7683965 | MS.gene26496:CDS |
| ACAACCTTGGAAGTAGCATG+GGG | 0.697964 | 7.3:-7684261 | MS.gene26496:CDS |
| ATACCACATTCAAATTCGAG+TGG | 0.714863 | 7.3:-7683740 | MS.gene26496:CDS |
| AAATGCCCCAGATTTCCTCG+AGG | 0.761109 | 7.3:+7684019 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTTTGTTGACAATAATTA+CGG | - | chr7.3:7684879-7684898 | MS.gene26496:CDS | 20.0% |
| !! | CAAAATCTTCAAAGTTAAAT+TGG | + | chr7.3:7684512-7684531 | None:intergenic | 20.0% |
| !! | TTGTGTAAAGTAAAAGATAT+TGG | + | chr7.3:7683802-7683821 | None:intergenic | 20.0% |
| !!! | AGTTTTATTCTCAATCAATT+TGG | + | chr7.3:7683311-7683330 | None:intergenic | 20.0% |
| !!! | ATATCTTTTACTTTACACAA+CGG | - | chr7.3:7683802-7683821 | MS.gene26496:CDS | 20.0% |
| !!! | TTTCTTTGTTGATCTTTTTA+AGG | + | chr7.3:7683588-7683607 | None:intergenic | 20.0% |
| ! | ACTTTGTTGACAATAATTAC+GGG | - | chr7.3:7684880-7684899 | MS.gene26496:CDS | 25.0% |
| ! | AGAAAATGAAGAGACTTTAA+TGG | - | chr7.3:7683603-7683622 | MS.gene26496:CDS | 25.0% |
| ! | ATAATATATGTAGCAACATG+TGG | - | chr7.3:7684748-7684767 | MS.gene26496:CDS | 25.0% |
| ! | GAAAATGAAGAGACTTTAAT+GGG | - | chr7.3:7683604-7683623 | MS.gene26496:CDS | 25.0% |
| !! | ACTTTTCATCAAAACAACTT+TGG | - | chr7.3:7684816-7684835 | MS.gene26496:CDS | 25.0% |
| !! | GGGCATTTTATTAACAATAT+GGG | - | chr7.3:7684186-7684205 | MS.gene26496:CDS | 25.0% |
| !!! | GGAAAATTTTGATAGTATCA+TGG | - | chr7.3:7684098-7684117 | MS.gene26496:CDS | 25.0% |
| ATGCATCAAATTGAAAACGA+AGG | - | chr7.3:7683634-7683653 | MS.gene26496:CDS | 30.0% | |
| ATGTCTAGGCATTTAGAAAA+GGG | - | chr7.3:7683739-7683758 | MS.gene26496:CDS | 30.0% | |
| GAAGTTCAAGAAAACTTTAG+AGG | - | chr7.3:7684717-7684736 | MS.gene26496:CDS | 30.0% | |
| GCTATAAGAGAAGATTGAAA+GGG | - | chr7.3:7683344-7683363 | MS.gene26496:CDS | 30.0% | |
| GTGATACAAAACCATCAATA+TGG | - | chr7.3:7683437-7683456 | MS.gene26496:CDS | 30.0% | |
| TATCCTCCAAAAAAGTCAAT+TGG | + | chr7.3:7683543-7683562 | None:intergenic | 30.0% | |
| TGAGAATAAAACTCGAAGAA+AGG | - | chr7.3:7683318-7683337 | MS.gene26496:CDS | 30.0% | |
| TGCTATAAGAGAAGATTGAA+AGG | - | chr7.3:7683343-7683362 | MS.gene26496:CDS | 30.0% | |
| TTGACTCAAACTTGGTTATT+AGG | + | chr7.3:7683486-7683505 | None:intergenic | 30.0% | |
| TTTCATCAAAACAACTTTGG+TGG | - | chr7.3:7684819-7684838 | MS.gene26496:CDS | 30.0% | |
| TTTGAAGGTCAAGTAAACTT+TGG | - | chr7.3:7684393-7684412 | MS.gene26496:CDS | 30.0% | |
| ! | AACTTTGGTGGCAATATTAA+TGG | - | chr7.3:7684831-7684850 | MS.gene26496:CDS | 30.0% |
| ! | TGACCTTCAAAATTTTCTTG+AGG | + | chr7.3:7684384-7684403 | None:intergenic | 30.0% |
| ! | TTGCTTCCTCAAGAAAATTT+TGG | - | chr7.3:7684483-7684502 | MS.gene26496:CDS | 30.0% |
| ! | TTGGCTAATCAAGCTAATTT+TGG | - | chr7.3:7683994-7684013 | MS.gene26496:CDS | 30.0% |
| ! | TTGTCTCATCAAGGAAATTT+TGG | - | chr7.3:7684600-7684619 | MS.gene26496:CDS | 30.0% |
| !! | GAAGATTTTGATATCAACAC+AGG | - | chr7.3:7684522-7684541 | MS.gene26496:CDS | 30.0% |
| !! | GGGGCATTTTATTAACAATA+TGG | - | chr7.3:7684185-7684204 | MS.gene26496:CDS | 30.0% |
| !!! | AGCGTTTTTTCAAGACAATT+TGG | - | chr7.3:7684077-7684096 | MS.gene26496:CDS | 30.0% |
| !!! | ATTGACTTTTTTGGAGGATA+AGG | - | chr7.3:7683543-7683562 | MS.gene26496:CDS | 30.0% |
| !!! | GGTGGTTTTGATAATAGAAT+GGG | - | chr7.3:7684015-7684034 | MS.gene26496:CDS | 30.0% |
| !!! | GTGGTTTTGATAATAGAATG+GGG | - | chr7.3:7684016-7684035 | MS.gene26496:CDS | 30.0% |
| !!! | TGGTGGTTTTGATAATAGAA+TGG | - | chr7.3:7684014-7684033 | MS.gene26496:CDS | 30.0% |
| !!! | TTGACTTTTTTGGAGGATAA+GGG | - | chr7.3:7683544-7683563 | MS.gene26496:CDS | 30.0% |
| AAACCACCACTAGAATATTC+AGG | + | chr7.3:7684051-7684070 | None:intergenic | 35.0% | |
| AACAATATGGGTAGCAACAT+TGG | - | chr7.3:7684198-7684217 | MS.gene26496:CDS | 35.0% | |
| ACAATATGGGTAGCAACATT+GGG | - | chr7.3:7684199-7684218 | MS.gene26496:CDS | 35.0% | |
| AGATCACTAGAAAGATGTCT+AGG | - | chr7.3:7683725-7683744 | MS.gene26496:CDS | 35.0% | |
| AGTGGTTTCAATAACAACCT+TGG | - | chr7.3:7683916-7683935 | MS.gene26496:CDS | 35.0% | |
| ATACCACATTCAAATTCGAG+TGG | - | chr7.3:7684450-7684469 | MS.gene26496:CDS | 35.0% | |
| ATACCACCACATACAAATCT+TGG | - | chr7.3:7684558-7684577 | MS.gene26496:CDS | 35.0% | |
| ATATTAATGGAGATGGGATG+TGG | - | chr7.3:7684844-7684863 | MS.gene26496:CDS | 35.0% | |
| CAAGTTTGAGTCAATGTCAT+TGG | - | chr7.3:7683492-7683511 | MS.gene26496:CDS | 35.0% | |
| CAATCACATGAAAATCCTAG+TGG | - | chr7.3:7684225-7684244 | MS.gene26496:CDS | 35.0% | |
| CCATCACATGAAAATCATAG+TGG | - | chr7.3:7684126-7684145 | MS.gene26496:CDS | 35.0% | |
| GACAATCTTATGTGGTATTG+AGG | - | chr7.3:7683390-7683409 | MS.gene26496:CDS | 35.0% | |
| GATGTCTAGGCATTTAGAAA+AGG | - | chr7.3:7683738-7683757 | MS.gene26496:CDS | 35.0% | |
| GTGGCAATATTAATGGAGAT+GGG | - | chr7.3:7684838-7684857 | MS.gene26496:CDS | 35.0% | |
| TACCACATGCAAATCTGAAT+GGG | - | chr7.3:7684340-7684359 | MS.gene26496:CDS | 35.0% | |
| TGTCTAGGCATTTAGAAAAG+GGG | - | chr7.3:7683740-7683759 | MS.gene26496:CDS | 35.0% | |
| TTACTTTACACAACGGTGAT+GGG | - | chr7.3:7683809-7683828 | MS.gene26496:CDS | 35.0% | |
| TTTACTTTACACAACGGTGA+TGG | - | chr7.3:7683808-7683827 | MS.gene26496:CDS | 35.0% | |
| ! | AAAGTTGATCAAATGAGCAG+TGG | - | chr7.3:7683898-7683917 | MS.gene26496:CDS | 35.0% |
| ! | CCACTATGATTTTCATGTGA+TGG | + | chr7.3:7684129-7684148 | None:intergenic | 35.0% |
| ! | CCTCCTCAAGAAAATTTTGA+AGG | - | chr7.3:7684378-7684397 | MS.gene26496:CDS | 35.0% |
| ! | CCTTCAAAATTTTCTTGAGG+AGG | + | chr7.3:7684381-7684400 | None:intergenic | 35.0% |
| ! | CTTCCTCAAGAAAATTTTGG+AGG | - | chr7.3:7684486-7684505 | MS.gene26496:CDS | 35.0% |
| ! | GCTAATCAAGCTAATTTTGG+TGG | - | chr7.3:7683997-7684016 | MS.gene26496:CDS | 35.0% |
| ! | TTTGATGACAACACATGTAG+CGG | - | chr7.3:7684420-7684439 | MS.gene26496:CDS | 35.0% |
| !! | CAAGGTTTTGATGACATTAC+AGG | - | chr7.3:7684300-7684319 | MS.gene26496:CDS | 35.0% |
| !! | CAATGACATTGACTCAAACT+TGG | + | chr7.3:7683494-7683513 | None:intergenic | 35.0% |
| !! | GTAAAAGATATTGGTGCCAA+CGG | + | chr7.3:7683793-7683812 | None:intergenic | 35.0% |
| !! | TTTGGAGTTGATAACAACAC+AGG | - | chr7.3:7684618-7684637 | MS.gene26496:CDS | 35.0% |
| !!! | ATTGATGGTTTTGTATCACC+TGG | + | chr7.3:7683436-7683455 | None:intergenic | 35.0% |
| !!! | GACTGACCAATTGACTTTTT+TGG | - | chr7.3:7683534-7683553 | MS.gene26496:CDS | 35.0% |
| !!! | TGACCAATTGACTTTTTTGG+AGG | - | chr7.3:7683537-7683556 | MS.gene26496:CDS | 35.0% |
| AACAACCTTGGAAGTAGCAT+GGG | - | chr7.3:7683928-7683947 | MS.gene26496:CDS | 40.0% | |
| ACACCAAGATTTGTATGTGG+TGG | + | chr7.3:7684564-7684583 | None:intergenic | 40.0% | |
| ACATTACAGGTAGCAACACA+GGG | - | chr7.3:7684313-7684332 | MS.gene26496:CDS | 40.0% | |
| ATACCACCTCATACAAATCC+TGG | - | chr7.3:7684654-7684673 | MS.gene26496:CDS | 40.0% | |
| ATATCAACACCACCATTGCT+TGG | + | chr7.3:7684792-7684811 | None:intergenic | 40.0% | |
| CAACAACCCTTGTCAGATTT+GGG | - | chr7.3:7683871-7683890 | MS.gene26496:CDS | 40.0% | |
| CATGAAAATCCAAGCAATGG+TGG | - | chr7.3:7684780-7684799 | MS.gene26496:CDS | 40.0% | |
| CATGATATGTTTCTTCCTCG+AGG | - | chr7.3:7684156-7684175 | MS.gene26496:CDS | 40.0% | |
| CCAACACCAAGATTTGTATG+TGG | + | chr7.3:7684567-7684586 | None:intergenic | 40.0% | |
| CCACATGAAAATCCAAGCAA+TGG | - | chr7.3:7684777-7684796 | MS.gene26496:CDS | 40.0% | |
| CCACTAGAATATTCAGGTGA+TGG | + | chr7.3:7684045-7684064 | None:intergenic | 40.0% | |
| CCATCACCTGAATATTCTAG+TGG | - | chr7.3:7684042-7684061 | MS.gene26496:CDS | 40.0% | |
| CTACCACATGCAAATCTGAA+TGG | - | chr7.3:7684339-7684358 | MS.gene26496:CDS | 40.0% | |
| GAAAGGGTTGTTCACCAAAA+TGG | - | chr7.3:7683360-7683379 | MS.gene26496:CDS | 40.0% | |
| GAGCATGTGACAATCTTATG+TGG | - | chr7.3:7683382-7683401 | MS.gene26496:CDS | 40.0% | |
| GGTGGCAATATTAATGGAGA+TGG | - | chr7.3:7684837-7684856 | MS.gene26496:CDS | 40.0% | |
| GTTGATGTGTTGTCTCATCA+AGG | - | chr7.3:7684591-7684610 | MS.gene26496:CDS | 40.0% | |
| TAACAACCTTGGAAGTAGCA+TGG | - | chr7.3:7683927-7683946 | MS.gene26496:CDS | 40.0% | |
| TACTTTACACAACGGTGATG+GGG | - | chr7.3:7683810-7683829 | MS.gene26496:CDS | 40.0% | |
| TCACATGAAAATCCTAGTGG+TGG | - | chr7.3:7684228-7684247 | MS.gene26496:CDS | 40.0% | |
| TCACCTGAATATTCTAGTGG+TGG | - | chr7.3:7684045-7684064 | MS.gene26496:CDS | 40.0% | |
| TGACCTCCAAAATATCCTTG+AGG | + | chr7.3:7684270-7684289 | None:intergenic | 40.0% | |
| TTCCCATTCAGATTTGCATG+TGG | + | chr7.3:7684345-7684364 | None:intergenic | 40.0% | |
| TTTCTTCCTCGAGGAAATCT+GGG | - | chr7.3:7684165-7684184 | MS.gene26496:CDS | 40.0% | |
| ! | CATAACGGGGTTAACATGTA+TGG | - | chr7.3:7683835-7683854 | MS.gene26496:CDS | 40.0% |
| ! | CCACATACAAATCTTGGTGT+TGG | - | chr7.3:7684564-7684583 | MS.gene26496:CDS | 40.0% |
| ! | CCATTGCTTGGATTTTCATG+TGG | + | chr7.3:7684780-7684799 | None:intergenic | 40.0% |
| ! | GATTGTCACATGCTCCATTT+TGG | + | chr7.3:7683377-7683396 | None:intergenic | 40.0% |
| ! | TGGCCTCCAAAATTTTCTTG+AGG | + | chr7.3:7684492-7684511 | None:intergenic | 40.0% |
| ! | TTGCCTCCTCAAGGATATTT+TGG | - | chr7.3:7684261-7684280 | MS.gene26496:CDS | 40.0% |
| ! | TTGCTTGGATTTTCATGTGG+AGG | + | chr7.3:7684777-7684796 | None:intergenic | 40.0% |
| ACAACCTTGGAAGTAGCATG+GGG | - | chr7.3:7683929-7683948 | MS.gene26496:CDS | 45.0% | |
| ACACCAGGATTTGTATGAGG+TGG | + | chr7.3:7684660-7684679 | None:intergenic | 45.0% | |
| ACATCAACATCACCAACACC+AGG | + | chr7.3:7684579-7684598 | None:intergenic | 45.0% | |
| CCAAATCTGACAAGGGTTGT+TGG | + | chr7.3:7683873-7683892 | None:intergenic | 45.0% | |
| CCAACAACCCTTGTCAGATT+TGG | - | chr7.3:7683870-7683889 | MS.gene26496:CDS | 45.0% | |
| CCAACACCAGGATTTGTATG+AGG | + | chr7.3:7684663-7684682 | None:intergenic | 45.0% | |
| CCACATTCAAATTCGAGTGG+TGG | - | chr7.3:7684453-7684472 | MS.gene26496:CDS | 45.0% | |
| CCACCACTCGAATTTGAATG+TGG | + | chr7.3:7684456-7684475 | None:intergenic | 45.0% | |
| CCTCATACAAATCCTGGTGT+TGG | - | chr7.3:7684660-7684679 | MS.gene26496:CDS | 45.0% | |
| CCTCCAAAATATCCTTGAGG+AGG | + | chr7.3:7684267-7684286 | None:intergenic | 45.0% | |
| CTTCCACTTGCAGATCTTAG+TGG | - | chr7.3:7683955-7683974 | MS.gene26496:CDS | 45.0% | |
| GACATTACAGGTAGCAACAC+AGG | - | chr7.3:7684312-7684331 | MS.gene26496:CDS | 45.0% | |
| GGAGGTCAAAGCAACTTTCA+AGG | - | chr7.3:7684282-7684301 | MS.gene26496:CDS | 45.0% | |
| GGTGGAGGAGGATTATTAAG+AGG | + | chr7.3:7683772-7683791 | None:intergenic | 45.0% | |
| GTTTCTTCCTCGAGGAAATC+TGG | - | chr7.3:7684164-7684183 | MS.gene26496:CDS | 45.0% | |
| TAATAATCCTCCTCCACCGT+TGG | - | chr7.3:7683774-7683793 | MS.gene26496:CDS | 45.0% | |
| TTCTCGCCCAAATCTGACAA+GGG | + | chr7.3:7683880-7683899 | None:intergenic | 45.0% | |
| TTCTTCCTCGAGGAAATCTG+GGG | - | chr7.3:7684166-7684185 | MS.gene26496:CDS | 45.0% | |
| TTTCTCGCCCAAATCTGACA+AGG | + | chr7.3:7683881-7683900 | None:intergenic | 45.0% | |
| ! | ATGTGGTATTGAGGCATGTG+CGG | - | chr7.3:7683399-7683418 | MS.gene26496:CDS | 45.0% |
| ! | GAAAATCCTAGTGGTGGTGT+AGG | - | chr7.3:7684234-7684253 | MS.gene26496:CDS | 45.0% |
| !! | AAAGATATTGGTGCCAACGG+TGG | + | chr7.3:7683790-7683809 | None:intergenic | 45.0% |
| !! | CCTCCTCAAGGATATTTTGG+AGG | - | chr7.3:7684264-7684283 | MS.gene26496:CDS | 45.0% |
| !! | TGGTGGTGGTTGTGATAACT+TGG | - | chr7.3:7683975-7683994 | MS.gene26496:CDS | 45.0% |
| AAATGCCCCAGATTTCCTCG+AGG | + | chr7.3:7684174-7684193 | None:intergenic | 50.0% | |
| AACACACCTACACCACCACT+AGG | + | chr7.3:7684243-7684262 | None:intergenic | 50.0% | |
| AATATGGCCATCTCCTGCAG+TGG | - | chr7.3:7683453-7683472 | MS.gene26496:CDS | 50.0% | |
| ACAACACATGTAGCGGCATG+TGG | - | chr7.3:7684427-7684446 | MS.gene26496:CDS | 50.0% | |
| AGGCATGTGCGGTTGTGTTT+GGG | - | chr7.3:7683410-7683429 | MS.gene26496:CDS | 50.0% | |
| ATTAGGTCTTCAGCCACTGC+AGG | + | chr7.3:7683469-7683488 | None:intergenic | 50.0% | |
| CCACCACTAAGATCTGCAAG+TGG | + | chr7.3:7683961-7683980 | None:intergenic | 50.0% | |
| CCACTTGCAGATCTTAGTGG+TGG | - | chr7.3:7683958-7683977 | MS.gene26496:CDS | 50.0% | |
| CTTGCAGATCTTAGTGGTGG+TGG | - | chr7.3:7683961-7683980 | MS.gene26496:CDS | 50.0% | |
| GCAGGAGATGGCCATATTGA+TGG | + | chr7.3:7683451-7683470 | None:intergenic | 50.0% | |
| ! | GTATCCCCATGCTACTTCCA+AGG | + | chr7.3:7683936-7683955 | None:intergenic | 50.0% |
| !! | AACGGTGATGGGGTGCATAA+CGG | - | chr7.3:7683820-7683839 | MS.gene26496:CDS | 50.0% |
| GAGGCATGTGCGGTTGTGTT+TGG | - | chr7.3:7683409-7683428 | MS.gene26496:CDS | 55.0% | |
| GTAGGTGTGTTGCCTCCTCA+AGG | - | chr7.3:7684252-7684271 | MS.gene26496:CDS | 55.0% | |
| TACGGGAGCAACTTCAACCC+TGG | - | chr7.3:7684897-7684916 | MS.gene26496:CDS | 55.0% | |
| TCTTCAGCCACTGCAGGAGA+TGG | + | chr7.3:7683463-7683482 | None:intergenic | 55.0% | |
| !! | ACGGTGATGGGGTGCATAAC+GGG | - | chr7.3:7683821-7683840 | MS.gene26496:CDS | 55.0% |
| !! | GATATTGGTGCCAACGGTGG+AGG | + | chr7.3:7683787-7683806 | None:intergenic | 55.0% |
| TGTGCGGTTGTGTTTGGGCC+AGG | - | chr7.3:7683415-7683434 | MS.gene26496:CDS | 60.0% | |
| !! | ATTGGTGCCAACGGTGGAGG+AGG | + | chr7.3:7683784-7683803 | None:intergenic | 60.0% |
| !! | CGGTGATGGGGTGCATAACG+GGG | - | chr7.3:7683822-7683841 | MS.gene26496:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 7683277 | 7684935 | 7683277 | ID=MS.gene26496 |
| chr7.3 | mRNA | 7683277 | 7684935 | 7683277 | ID=MS.gene26496.t1;Parent=MS.gene26496 |
| chr7.3 | exon | 7683277 | 7684935 | 7683277 | ID=MS.gene26496.t1.exon1;Parent=MS.gene26496.t1 |
| chr7.3 | CDS | 7683277 | 7684935 | 7683277 | ID=cds.MS.gene26496.t1;Parent=MS.gene26496.t1 |
| Gene Sequence |
| Protein sequence |
