Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26689.t1 | XP_024636232.1 | 94.6 | 186 | 10 | 0 | 1 | 186 | 1 | 186 | 4.60E-99 | 370.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26689.t1 | A0A2K3M5A0 | 79.7 | 192 | 38 | 1 | 1 | 191 | 179 | 370 | 2.5e-86 | 327.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052973 | MS.gene26689 | 0.889214 | 2.96E-73 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26689.t1 | MTR_8g073195 | 36.190 | 105 | 64 | 2 | 86 | 190 | 43 | 144 | 1.56e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26689.t1 | AT3G30525 | 42.276 | 123 | 65 | 2 | 1 | 118 | 37 | 158 | 2.70e-28 | 104 |
| MS.gene26689.t1 | AT5G35695 | 37.226 | 137 | 73 | 2 | 2 | 137 | 24 | 148 | 7.43e-27 | 102 |
| MS.gene26689.t1 | AT5G41980 | 29.843 | 191 | 115 | 4 | 3 | 186 | 185 | 363 | 8.67e-25 | 99.8 |
| MS.gene26689.t1 | AT1G43722 | 43.810 | 105 | 53 | 2 | 1 | 100 | 216 | 319 | 9.11e-25 | 99.0 |
| MS.gene26689.t1 | AT4G10890 | 42.857 | 91 | 51 | 1 | 14 | 104 | 71 | 160 | 7.73e-19 | 84.0 |
| MS.gene26689.t1 | AT4G10890 | 42.857 | 91 | 51 | 1 | 14 | 104 | 71 | 160 | 7.73e-19 | 84.0 |
| MS.gene26689.t1 | AT4G10890 | 42.857 | 91 | 51 | 1 | 14 | 104 | 71 | 160 | 7.73e-19 | 84.0 |
| MS.gene26689.t1 | AT5G28730 | 43.077 | 65 | 36 | 1 | 1 | 65 | 150 | 213 | 7.64e-13 | 65.9 |
Find 32 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTCAATGACATTTCTA+AGG | 0.228551 | 8.4:+9738526 | None:intergenic |
| TCAAATAGCCACTAAGTTAT+TGG | 0.230945 | 8.4:-9738241 | MS.gene26689:CDS |
| GATAGATATCCTTTCATGTT+TGG | 0.305736 | 8.4:+9738642 | None:intergenic |
| CGAGCATATATCCTTGGATT+TGG | 0.329097 | 8.4:+9738352 | None:intergenic |
| TTTGTTGTTGTTGGCTGGCC+TGG | 0.350368 | 8.4:-9738887 | MS.gene26689:CDS |
| TCTTGTTTGATTAGTGGAAA+TGG | 0.370259 | 8.4:+9738456 | None:intergenic |
| TTGAAAGAACAATTGGTGTT+TGG | 0.373420 | 8.4:-9738512 | MS.gene26689:CDS |
| CTCGTTCGAGCATATATCCT+TGG | 0.409241 | 8.4:+9738346 | None:intergenic |
| AATGTCATTGAAAGAACAAT+TGG | 0.424218 | 8.4:-9738519 | MS.gene26689:CDS |
| AAGATGAGGAATTCAACAAA+TGG | 0.440534 | 8.4:-9738377 | MS.gene26689:CDS |
| TGACTGCCATCTCTAAAATC+AGG | 0.452335 | 8.4:+9738591 | None:intergenic |
| CAATTGGTGTTTGGAAGAAA+AGG | 0.453010 | 8.4:-9738503 | MS.gene26689:CDS |
| TGATATCTTTCACCTTTGTA+TGG | 0.453127 | 8.4:+9738618 | None:intergenic |
| AAGTATTACTTGGTTGATGC+AGG | 0.461050 | 8.4:-9738669 | MS.gene26689:CDS |
| CATAACTTCATTCGAATATG+TGG | 0.472793 | 8.4:-9738405 | MS.gene26689:CDS |
| ATTTCTAGGAAAGTATTACT+TGG | 0.498326 | 8.4:-9738679 | MS.gene26689:intron |
| ATCGTACCTTCAGGAGGATG+AGG | 0.518203 | 8.4:+9738806 | None:intergenic |
| AGATGAGGAATTCAACAAAT+GGG | 0.524003 | 8.4:-9738376 | MS.gene26689:CDS |
| TGTGTGTCATGCGCAGTACC+AGG | 0.524579 | 8.4:+9738869 | None:intergenic |
| TCTTTCAATGACATTTCTAA+GGG | 0.531707 | 8.4:+9738527 | None:intergenic |
| TATAATTTAAGAGTACAAGA+TGG | 0.540136 | 8.4:-9738288 | MS.gene26689:CDS |
| ATAATTTAAGAGTACAAGAT+GGG | 0.578232 | 8.4:-9738287 | MS.gene26689:CDS |
| AGTACAAGATGGGAGCTACA+TGG | 0.581291 | 8.4:-9738277 | MS.gene26689:CDS |
| AACAAATGGGACCAAATCCA+AGG | 0.586140 | 8.4:-9738363 | MS.gene26689:CDS |
| GTGTTCCCTCATCCTCCTGA+AGG | 0.587543 | 8.4:-9738812 | MS.gene26689:intron |
| GCAGGATATCCAAACATGAA+AGG | 0.592144 | 8.4:-9738651 | MS.gene26689:CDS |
| TTGGTGTTTGGAAGAAAAGG+TGG | 0.597185 | 8.4:-9738500 | MS.gene26689:CDS |
| AATATGTGGCGTTCAAGATG+AGG | 0.609133 | 8.4:-9738391 | MS.gene26689:CDS |
| TCGTACCTTCAGGAGGATGA+GGG | 0.629341 | 8.4:+9738807 | None:intergenic |
| AGAGATGGCAGTCAAGCACA+AGG | 0.648134 | 8.4:-9738582 | MS.gene26689:CDS |
| AGATTAATCGTACCTTCAGG+AGG | 0.657477 | 8.4:+9738800 | None:intergenic |
| GGATATCTATCACCATACAA+AGG | 0.686019 | 8.4:-9738630 | MS.gene26689:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTGATTTATATAGTATTACT+TGG | - | chr8.4:9738362-9738381 | MS.gene26689:CDS | 20.0% |
| !!! | ATAATTTAAGAGTACAAGAT+GGG | - | chr8.4:9738827-9738846 | MS.gene26689:CDS | 20.0% |
| !!! | TATAATTTAAGAGTACAAGA+TGG | - | chr8.4:9738826-9738845 | MS.gene26689:CDS | 20.0% |
| ! | AATGTCATTGAAAGAACAAT+TGG | - | chr8.4:9738595-9738614 | MS.gene26689:CDS | 25.0% |
| ! | TCTTTCAATGACATTTCTAA+GGG | + | chr8.4:9738590-9738609 | None:intergenic | 25.0% |
| ! | TTCTTTCAATGACATTTCTA+AGG | + | chr8.4:9738591-9738610 | None:intergenic | 25.0% |
| !! | ACACATTTTTCATTTCTTCA+AGG | + | chr8.4:9738286-9738305 | None:intergenic | 25.0% |
| !!! | ATCTTTTCTTGTTTGATTAG+TGG | + | chr8.4:9738667-9738686 | None:intergenic | 25.0% |
| !!! | ATTTCTAGGAAAGTATTACT+TGG | - | chr8.4:9738435-9738454 | MS.gene26689:CDS | 25.0% |
| !!! | TTAATCACTTTTGTTGTTGT+TGG | - | chr8.4:9738218-9738237 | MS.gene26689:CDS | 25.0% |
| !!! | TTTGTTTTCCAATAACTTAG+TGG | + | chr8.4:9738884-9738903 | None:intergenic | 25.0% |
| AAAAGATTAATCGTACCTTC+AGG | + | chr8.4:9738320-9738339 | None:intergenic | 30.0% | |
| AAGATGAGGAATTCAACAAA+TGG | - | chr8.4:9738737-9738756 | MS.gene26689:intron | 30.0% | |
| AGATGAGGAATTCAACAAAT+GGG | - | chr8.4:9738738-9738757 | MS.gene26689:intron | 30.0% | |
| CATAACTTCATTCGAATATG+TGG | - | chr8.4:9738709-9738728 | MS.gene26689:intron | 30.0% | |
| GATAGATATCCTTTCATGTT+TGG | + | chr8.4:9738475-9738494 | None:intergenic | 30.0% | |
| TCAAATAGCCACTAAGTTAT+TGG | - | chr8.4:9738873-9738892 | MS.gene26689:CDS | 30.0% | |
| TGATATCTTTCACCTTTGTA+TGG | + | chr8.4:9738499-9738518 | None:intergenic | 30.0% | |
| ! | TCTTGTTTGATTAGTGGAAA+TGG | + | chr8.4:9738661-9738680 | None:intergenic | 30.0% |
| ! | TTGAAAGAACAATTGGTGTT+TGG | - | chr8.4:9738602-9738621 | MS.gene26689:CDS | 30.0% |
| GGATATCTATCACCATACAA+AGG | - | chr8.4:9738484-9738503 | MS.gene26689:CDS | 35.0% | |
| ! | AAGTATTACTTGGTTGATGC+AGG | - | chr8.4:9738445-9738464 | MS.gene26689:CDS | 35.0% |
| ! | CAATTGGTGTTTGGAAGAAA+AGG | - | chr8.4:9738611-9738630 | MS.gene26689:CDS | 35.0% |
| !! | CACATTCCTGATTTTAGAGA+TGG | - | chr8.4:9738517-9738536 | MS.gene26689:CDS | 35.0% |
| AACAAATGGGACCAAATCCA+AGG | - | chr8.4:9738751-9738770 | MS.gene26689:intron | 40.0% | |
| AATATGTGGCGTTCAAGATG+AGG | - | chr8.4:9738723-9738742 | MS.gene26689:intron | 40.0% | |
| AGATTAATCGTACCTTCAGG+AGG | + | chr8.4:9738317-9738336 | None:intergenic | 40.0% | |
| CGAGCATATATCCTTGGATT+TGG | + | chr8.4:9738765-9738784 | None:intergenic | 40.0% | |
| GCAGGATATCCAAACATGAA+AGG | - | chr8.4:9738463-9738482 | MS.gene26689:CDS | 40.0% | |
| ! | TTGGTGTTTGGAAGAAAAGG+TGG | - | chr8.4:9738614-9738633 | MS.gene26689:CDS | 40.0% |
| !! | TGACTGCCATCTCTAAAATC+AGG | + | chr8.4:9738526-9738545 | None:intergenic | 40.0% |
| !!! | TCACTTTTGTTGTTGTTGGC+TGG | - | chr8.4:9738222-9738241 | MS.gene26689:CDS | 40.0% |
| AGTACAAGATGGGAGCTACA+TGG | - | chr8.4:9738837-9738856 | MS.gene26689:CDS | 45.0% | |
| CTCGTTCGAGCATATATCCT+TGG | + | chr8.4:9738771-9738790 | None:intergenic | 45.0% | |
| !!! | TTTTATTTTTATATATTTCT+AGG | - | chr8.4:9738421-9738440 | MS.gene26689:CDS | 5.0% |
| AGAGATGGCAGTCAAGCACA+AGG | - | chr8.4:9738532-9738551 | MS.gene26689:CDS | 50.0% | |
| ATCGTACCTTCAGGAGGATG+AGG | + | chr8.4:9738311-9738330 | None:intergenic | 50.0% | |
| TCGTACCTTCAGGAGGATGA+GGG | + | chr8.4:9738310-9738329 | None:intergenic | 50.0% | |
| !! | TTTGTTGTTGTTGGCTGGCC+TGG | - | chr8.4:9738227-9738246 | MS.gene26689:CDS | 50.0% |
| GTGTTCCCTCATCCTCCTGA+AGG | - | chr8.4:9738302-9738321 | MS.gene26689:CDS | 55.0% | |
| TGTGTGTCATGCGCAGTACC+AGG | + | chr8.4:9738248-9738267 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9738215 | 9738921 | 9738215 | ID=MS.gene26689 |
| chr8.4 | mRNA | 9738215 | 9738921 | 9738215 | ID=MS.gene26689.t1;Parent=MS.gene26689 |
| chr8.4 | exon | 9738813 | 9738921 | 9738813 | ID=MS.gene26689.t1.exon1;Parent=MS.gene26689.t1 |
| chr8.4 | CDS | 9738813 | 9738921 | 9738813 | ID=cds.MS.gene26689.t1;Parent=MS.gene26689.t1 |
| chr8.4 | exon | 9738215 | 9738693 | 9738215 | ID=MS.gene26689.t1.exon2;Parent=MS.gene26689.t1 |
| chr8.4 | CDS | 9738215 | 9738693 | 9738215 | ID=cds.MS.gene26689.t1;Parent=MS.gene26689.t1 |
| Gene Sequence |
| Protein sequence |
