Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26893.t1 | XP_024626066.1 | 93.7 | 205 | 13 | 0 | 1 | 205 | 1 | 205 | 2.00E-97 | 365.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26893.t1 | Q9ZVZ9 | 67.1 | 155 | 45 | 1 | 57 | 205 | 45 | 199 | 4.7e-50 | 199.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26893.t1 | A0A072W0T5 | 93.7 | 205 | 13 | 0 | 1 | 205 | 1 | 205 | 1.5e-97 | 365.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05702 | MS.gene26893 | 0.801532 | 8.61E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26893.t1 | MTR_1g103810 | 93.659 | 205 | 13 | 0 | 1 | 205 | 1 | 205 | 6.26e-140 | 389 |
| MS.gene26893.t1 | MTR_1g103810 | 93.125 | 160 | 11 | 0 | 1 | 160 | 1 | 160 | 9.97e-107 | 303 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26893.t1 | AT1G05385 | 67.097 | 155 | 45 | 1 | 57 | 205 | 45 | 199 | 4.54e-68 | 207 |
| MS.gene26893.t1 | AT1G05385 | 72.028 | 143 | 37 | 1 | 63 | 205 | 6 | 145 | 3.84e-67 | 202 |
Find 46 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCAGCCCATACCATTAA+TGG | 0.191460 | 1.4:-80280071 | None:intergenic |
| TTACGGTTTATCACCATTAA+TGG | 0.231145 | 1.4:+80280058 | MS.gene26893:CDS |
| TCCTGACTTTGTCTTCTCAT+TGG | 0.243643 | 1.4:-80280159 | None:intergenic |
| GAAAATGATGATGGGGTTAT+TGG | 0.251430 | 1.4:+80280113 | MS.gene26893:CDS |
| CCTGACTTTGTCTTCTCATT+GGG | 0.271792 | 1.4:-80280158 | None:intergenic |
| ATCTGCAGTCCGTCTAAATT+TGG | 0.273557 | 1.4:-80280270 | None:intergenic |
| GGTAAAAGCCGTAAGATCTT+TGG | 0.281637 | 1.4:+80280511 | MS.gene26893:CDS |
| CTAAATTTGGCATTGTCATC+AGG | 0.305219 | 1.4:-80280257 | None:intergenic |
| AATCAGCGTCGGCACTTCAT+TGG | 0.383394 | 1.4:+80280019 | MS.gene26893:CDS |
| TCAGGAATCATATGTTGCTT+TGG | 0.386912 | 1.4:+80280490 | MS.gene26893:intron |
| TGCAGCAAAGGAGTCGATTC+GGG | 0.402932 | 1.4:+80280292 | MS.gene26893:CDS |
| GTTTATCACCATTAATGGTA+TGG | 0.415525 | 1.4:+80280063 | MS.gene26893:CDS |
| GCTGACTTTAAGTAAGCCTT+TGG | 0.427756 | 1.4:-80280191 | None:intergenic |
| GACTTAACTTCTGTCGGCAG+TGG | 0.434510 | 1.4:-80280566 | None:intergenic |
| ATGCAGCAAAGGAGTCGATT+CGG | 0.434911 | 1.4:+80280291 | MS.gene26893:CDS |
| CCCAATGAGAAGACAAAGTC+AGG | 0.437695 | 1.4:+80280158 | MS.gene26893:CDS |
| GGAACACATAATAGCTGAAA+AGG | 0.441979 | 1.4:-80279239 | None:intergenic |
| TTCGGGAGTATCTTGGTAGT+TGG | 0.447521 | 1.4:+80280309 | MS.gene26893:CDS |
| GTTACAATAACATGCTGTTA+CGG | 0.462256 | 1.4:+80280041 | MS.gene26893:CDS |
| ATCAAAAGATTTGACATTGA+AGG | 0.466070 | 1.4:+80279280 | MS.gene26893:CDS |
| ATACTTCTTGTGCAACAGTC+TGG | 0.475696 | 1.4:-80280340 | None:intergenic |
| AAAGTTTGCCAAAGATCTTA+CGG | 0.486034 | 1.4:-80280519 | None:intergenic |
| TCTGTCGGCAGTGGGGCTGA+TGG | 0.489076 | 1.4:-80280557 | None:intergenic |
| GACAAAGAGGAAAATGATGA+TGG | 0.495002 | 1.4:+80280104 | MS.gene26893:CDS |
| TAACAGATAAACTGCAATCC+AGG | 0.501310 | 1.4:+80279946 | MS.gene26893:intron |
| GGGAGTATCTTGGTAGTTGG+AGG | 0.505346 | 1.4:+80280312 | MS.gene26893:CDS |
| ACTTAACTTCTGTCGGCAGT+GGG | 0.516664 | 1.4:-80280565 | None:intergenic |
| TTTATCACCATTAATGGTAT+GGG | 0.518196 | 1.4:+80280064 | MS.gene26893:CDS |
| ATTTCAGACTTAACTTCTGT+CGG | 0.518402 | 1.4:-80280572 | None:intergenic |
| GAGTCGATTCGGGAGTATCT+TGG | 0.529772 | 1.4:+80280302 | MS.gene26893:CDS |
| GATACCTTCAGAACTTGTGA+TGG | 0.534488 | 1.4:-80279260 | None:intergenic |
| TAAATTTGGCATTGTCATCA+GGG | 0.539047 | 1.4:-80280256 | None:intergenic |
| TTACTTAAAGTCAGCAAGAG+AGG | 0.560221 | 1.4:+80280199 | MS.gene26893:CDS |
| AGAGAAGTCAGCTGACAAAG+AGG | 0.561684 | 1.4:+80280091 | MS.gene26893:CDS |
| ACAAAGAGGAAAATGATGAT+GGG | 0.572295 | 1.4:+80280105 | MS.gene26893:CDS |
| GGAGTATCTTGGTAGTTGGA+GGG | 0.581542 | 1.4:+80280313 | MS.gene26893:CDS |
| TGTTCCATCACAAGTTCTGA+AGG | 0.589057 | 1.4:+80279256 | MS.gene26893:CDS |
| ACGGACTGCAGATGCAGCAA+AGG | 0.596536 | 1.4:+80280280 | MS.gene26893:CDS |
| AAGCAGAGCAATATTAGACC+TGG | 0.607183 | 1.4:-80279964 | None:intergenic |
| ATGACAATGCCAAATTTAGA+CGG | 0.613681 | 1.4:+80280261 | MS.gene26893:CDS |
| AAGTGCCGACGCTGATTGAA+AGG | 0.617115 | 1.4:-80280013 | None:intergenic |
| CAAAGAGGAAAATGATGATG+GGG | 0.618349 | 1.4:+80280106 | MS.gene26893:CDS |
| GAGTATCTTGGTAGTTGGAG+GGG | 0.662716 | 1.4:+80280314 | MS.gene26893:CDS |
| AGACACCTTTCAATCAGCGT+CGG | 0.679519 | 1.4:+80280008 | MS.gene26893:CDS |
| CTTAACTTCTGTCGGCAGTG+GGG | 0.694539 | 1.4:-80280564 | None:intergenic |
| CTACGACTAGAAGCTTCGAG+AGG | 0.724826 | 1.4:-80279987 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATATATAATCTTGCTT+TGG | + | chr1.4:80280456-80280475 | MS.gene26893:intron | 15.0% |
| !! | AATATATATAATCTTGCTTT+GGG | + | chr1.4:80280457-80280476 | MS.gene26893:intron | 15.0% |
| !! | TTTGCAAAAAAAAAAAAAAC+TGG | - | chr1.4:80280439-80280458 | None:intergenic | 15.0% |
| !! | AATAAATTGTATCGTTGAAT+TGG | + | chr1.4:80279504-80279523 | MS.gene26893:intron | 20.0% |
| !! | AATAAGCTAAAAACAACTTA+TGG | - | chr1.4:80279683-80279702 | None:intergenic | 20.0% |
| !! | ATAAATTGTATCGTTGAATT+GGG | + | chr1.4:80279505-80279524 | MS.gene26893:intron | 20.0% |
| !! | ATAAGCTAAAAACAACTTAT+GGG | - | chr1.4:80279682-80279701 | None:intergenic | 20.0% |
| !!! | AAAAAGTGTTTGAAATAGTA+AGG | - | chr1.4:80279311-80279330 | None:intergenic | 20.0% |
| !!! | ACTGTTTTCATAAATTATTC+TGG | - | chr1.4:80279718-80279737 | None:intergenic | 20.0% |
| !!! | TAGTATATTTGTTAGCATTT+TGG | + | chr1.4:80280407-80280426 | MS.gene26893:intron | 20.0% |
| ! | AGCGAGTATAATTTAACAAA+AGG | - | chr1.4:80279334-80279353 | None:intergenic | 25.0% |
| ! | ATACATAAATAGAATGCAAG+TGG | + | chr1.4:80279437-80279456 | MS.gene26893:intron | 25.0% |
| ! | ATCAAAAGATTTGACATTGA+AGG | + | chr1.4:80279280-80279299 | MS.gene26893:CDS | 25.0% |
| ! | TTTATCACCATTAATGGTAT+GGG | + | chr1.4:80280064-80280083 | MS.gene26893:CDS | 25.0% |
| AAAGTTTGCCAAAGATCTTA+CGG | - | chr1.4:80280522-80280541 | None:intergenic | 30.0% | |
| ACAAAGAGGAAAATGATGAT+GGG | + | chr1.4:80280105-80280124 | MS.gene26893:CDS | 30.0% | |
| ATGACAATGCCAAATTTAGA+CGG | + | chr1.4:80280261-80280280 | MS.gene26893:CDS | 30.0% | |
| ATGATAAACCTAGTAACAGT+AGG | + | chr1.4:80279892-80279911 | MS.gene26893:intron | 30.0% | |
| ATTTCAGACTTAACTTCTGT+CGG | - | chr1.4:80280575-80280594 | None:intergenic | 30.0% | |
| GTTACAATAACATGCTGTTA+CGG | + | chr1.4:80280041-80280060 | MS.gene26893:CDS | 30.0% | |
| GTTTATCACCATTAATGGTA+TGG | + | chr1.4:80280063-80280082 | MS.gene26893:CDS | 30.0% | |
| TAAATTTGGCATTGTCATCA+GGG | - | chr1.4:80280259-80280278 | None:intergenic | 30.0% | |
| TGAAATAAGTCAATGCAAAC+AGG | - | chr1.4:80279589-80279608 | None:intergenic | 30.0% | |
| TTACGGTTTATCACCATTAA+TGG | + | chr1.4:80280058-80280077 | MS.gene26893:CDS | 30.0% | |
| TTGTCATCCAAAAAGATTAG+CGG | - | chr1.4:80279476-80279495 | None:intergenic | 30.0% | |
| ! | GGGATCAAATAACGTTTTTA+CGG | - | chr1.4:80280141-80280160 | None:intergenic | 30.0% |
| ! | TTTGGCAAACTTTTACTCAA+AGG | + | chr1.4:80280529-80280548 | MS.gene26893:CDS | 30.0% |
| ACGCATTTGTAAGACTGTTT+GGG | + | chr1.4:80279625-80279644 | MS.gene26893:intron | 35.0% | |
| ATAGGGACGTGTTTACATAT+AGG | - | chr1.4:80279362-80279381 | None:intergenic | 35.0% | |
| ATATGTAAACACGAGGGAAA+GGG | + | chr1.4:80279380-80279399 | MS.gene26893:intron | 35.0% | |
| CTAAATTTGGCATTGTCATC+AGG | - | chr1.4:80280260-80280279 | None:intergenic | 35.0% | |
| GAAAAGCTGTCAATAAACGA+AGG | - | chr1.4:80279919-80279938 | None:intergenic | 35.0% | |
| GACAAAGAGGAAAATGATGA+TGG | + | chr1.4:80280104-80280123 | MS.gene26893:CDS | 35.0% | |
| GGAACACATAATAGCTGAAA+AGG | - | chr1.4:80279242-80279261 | None:intergenic | 35.0% | |
| GTCCTGAGGAAACAATTTAA+GGG | - | chr1.4:80279567-80279586 | None:intergenic | 35.0% | |
| TAACAGATAAACTGCAATCC+AGG | + | chr1.4:80279946-80279965 | MS.gene26893:intron | 35.0% | |
| TAGGGACGTGTTTACATATA+GGG | - | chr1.4:80279361-80279380 | None:intergenic | 35.0% | |
| TATATGTAAACACGAGGGAA+AGG | + | chr1.4:80279379-80279398 | MS.gene26893:intron | 35.0% | |
| TCCTGAGGAAACAATTTAAG+GGG | - | chr1.4:80279566-80279585 | None:intergenic | 35.0% | |
| TTACTTAAAGTCAGCAAGAG+AGG | + | chr1.4:80280199-80280218 | MS.gene26893:CDS | 35.0% | |
| TTCCCTCGTGTTTACATATA+GGG | - | chr1.4:80279379-80279398 | None:intergenic | 35.0% | |
| TTTCCCTCGTGTTTACATAT+AGG | - | chr1.4:80279380-80279399 | None:intergenic | 35.0% | |
| ! | CAAACTTTTACTCAAAGGCA+GGG | + | chr1.4:80280534-80280553 | MS.gene26893:CDS | 35.0% |
| ! | CAAAGAGGAAAATGATGATG+GGG | + | chr1.4:80280106-80280125 | MS.gene26893:CDS | 35.0% |
| ! | TCAGGAATCATATGTTGCTT+TGG | + | chr1.4:80280490-80280509 | MS.gene26893:intron | 35.0% |
| !! | GAAAATGATGATGGGGTTAT+TGG | + | chr1.4:80280113-80280132 | MS.gene26893:CDS | 35.0% |
| AAGCAGAGCAATATTAGACC+TGG | - | chr1.4:80279967-80279986 | None:intergenic | 40.0% | |
| ATACTTCTTGTGCAACAGTC+TGG | - | chr1.4:80280343-80280362 | None:intergenic | 40.0% | |
| ATCTGCAGTCCGTCTAAATT+TGG | - | chr1.4:80280273-80280292 | None:intergenic | 40.0% | |
| CACGCATTTGTAAGACTGTT+TGG | + | chr1.4:80279624-80279643 | MS.gene26893:intron | 40.0% | |
| CCTGACTTTGTCTTCTCATT+GGG | - | chr1.4:80280161-80280180 | None:intergenic | 40.0% | |
| CGCATTTGTAAGACTGTTTG+GGG | + | chr1.4:80279626-80279645 | MS.gene26893:intron | 40.0% | |
| GACGTGTTTACATATAGGGA+CGG | - | chr1.4:80279357-80279376 | None:intergenic | 40.0% | |
| GATACCTTCAGAACTTGTGA+TGG | - | chr1.4:80279263-80279282 | None:intergenic | 40.0% | |
| GCTAAAAACAACTTATGGGC+AGG | - | chr1.4:80279678-80279697 | None:intergenic | 40.0% | |
| GGAAAGGGTGGTTACATATA+GGG | + | chr1.4:80279395-80279414 | MS.gene26893:intron | 40.0% | |
| GGTAAAAGCCGTAAGATCTT+TGG | + | chr1.4:80280511-80280530 | MS.gene26893:CDS | 40.0% | |
| GGTCCTGAGGAAACAATTTA+AGG | - | chr1.4:80279568-80279587 | None:intergenic | 40.0% | |
| GTCCCTATATGTAAACACGA+GGG | + | chr1.4:80279374-80279393 | MS.gene26893:intron | 40.0% | |
| TCCTGACTTTGTCTTCTCAT+TGG | - | chr1.4:80280162-80280181 | None:intergenic | 40.0% | |
| TCTTCAGCCCATACCATTAA+TGG | - | chr1.4:80280074-80280093 | None:intergenic | 40.0% | |
| TTGTATCGTTGAATTGGGAG+TGG | + | chr1.4:80279510-80279529 | MS.gene26893:intron | 40.0% | |
| ! | GCAAACTTTTACTCAAAGGC+AGG | + | chr1.4:80280533-80280552 | MS.gene26893:CDS | 40.0% |
| ! | GCTGACTTTAAGTAAGCCTT+TGG | - | chr1.4:80280194-80280213 | None:intergenic | 40.0% |
| ! | GTCAGGAAAAGTTTTGCCAA+AGG | + | chr1.4:80280175-80280194 | MS.gene26893:CDS | 40.0% |
| ! | TGTTCCATCACAAGTTCTGA+AGG | + | chr1.4:80279256-80279275 | MS.gene26893:CDS | 40.0% |
| !!! | GCTTTGGGTTGATTCTTTTC+AGG | + | chr1.4:80280472-80280491 | MS.gene26893:intron | 40.0% |
| AAGACTGTTTGGGGAAGTTG+TGG | + | chr1.4:80279635-80279654 | MS.gene26893:intron | 45.0% | |
| ACGAAGGACCTACTGTTACT+AGG | - | chr1.4:80279903-80279922 | None:intergenic | 45.0% | |
| ACTTAACTTCTGTCGGCAGT+GGG | - | chr1.4:80280568-80280587 | None:intergenic | 45.0% | |
| AGACACCTTTCAATCAGCGT+CGG | + | chr1.4:80280008-80280027 | MS.gene26893:CDS | 45.0% | |
| AGAGAAGTCAGCTGACAAAG+AGG | + | chr1.4:80280091-80280110 | MS.gene26893:CDS | 45.0% | |
| AGGGTGGTTACATATAGGGA+CGG | + | chr1.4:80279399-80279418 | MS.gene26893:intron | 45.0% | |
| ATGCAGCAAAGGAGTCGATT+CGG | + | chr1.4:80280291-80280310 | MS.gene26893:CDS | 45.0% | |
| CCCAATGAGAAGACAAAGTC+AGG | + | chr1.4:80280158-80280177 | MS.gene26893:CDS | 45.0% | |
| CGTCCCTATATGTAAACACG+AGG | + | chr1.4:80279355-80279374 | MS.gene26893:intron | 45.0% | |
| GCCCCTTAAATTGTTTCCTC+AGG | + | chr1.4:80279562-80279581 | MS.gene26893:intron | 45.0% | |
| GGGAAAGGGTGGTTACATAT+AGG | + | chr1.4:80279394-80279413 | MS.gene26893:intron | 45.0% | |
| TGGTTACATATAGGGACGGA+GGG | + | chr1.4:80279403-80279422 | MS.gene26893:intron | 45.0% | |
| ! | AGCTGCACCGCTAATCTTTT+TGG | + | chr1.4:80279466-80279485 | MS.gene26893:intron | 45.0% |
| ! | GAGTATCTTGGTAGTTGGAG+GGG | + | chr1.4:80280314-80280333 | MS.gene26893:CDS | 45.0% |
| ! | GGAGTATCTTGGTAGTTGGA+GGG | + | chr1.4:80280313-80280332 | MS.gene26893:CDS | 45.0% |
| !! | TTCGGGAGTATCTTGGTAGT+TGG | + | chr1.4:80280309-80280328 | MS.gene26893:CDS | 45.0% |
| CTACGACTAGAAGCTTCGAG+AGG | - | chr1.4:80279990-80280009 | None:intergenic | 50.0% | |
| CTTAACTTCTGTCGGCAGTG+GGG | - | chr1.4:80280567-80280586 | None:intergenic | 50.0% | |
| GACTTAACTTCTGTCGGCAG+TGG | - | chr1.4:80280569-80280588 | None:intergenic | 50.0% | |
| GTCAATGCAAACAGGTCCTG+AGG | - | chr1.4:80279581-80279600 | None:intergenic | 50.0% | |
| GTGGTTACATATAGGGACGG+AGG | + | chr1.4:80279402-80279421 | MS.gene26893:intron | 50.0% | |
| TGTAAACACGAGGGAAAGGG+TGG | + | chr1.4:80279383-80279402 | MS.gene26893:intron | 50.0% | |
| ! | TGCAGCAAAGGAGTCGATTC+GGG | + | chr1.4:80280292-80280311 | MS.gene26893:CDS | 50.0% |
| !! | AAGTGCCGACGCTGATTGAA+AGG | - | chr1.4:80280016-80280035 | None:intergenic | 50.0% |
| !! | AATCAGCGTCGGCACTTCAT+TGG | + | chr1.4:80280019-80280038 | MS.gene26893:CDS | 50.0% |
| !! | GAGTCGATTCGGGAGTATCT+TGG | + | chr1.4:80280302-80280321 | MS.gene26893:CDS | 50.0% |
| !! | GGGAGTATCTTGGTAGTTGG+AGG | + | chr1.4:80280312-80280331 | MS.gene26893:CDS | 50.0% |
| ! | ACGGACTGCAGATGCAGCAA+AGG | + | chr1.4:80280280-80280299 | MS.gene26893:CDS | 55.0% |
| TCTGTCGGCAGTGGGGCTGA+TGG | - | chr1.4:80280560-80280579 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 80279229 | 80280631 | 80279229 | ID=MS.gene26893 |
| chr1.4 | mRNA | 80279229 | 80280631 | 80279229 | ID=MS.gene26893.t1;Parent=MS.gene26893 |
| chr1.4 | exon | 80279229 | 80279301 | 80279229 | ID=MS.gene26893.t1.exon1;Parent=MS.gene26893.t1 |
| chr1.4 | CDS | 80279229 | 80279301 | 80279229 | ID=cds.MS.gene26893.t1;Parent=MS.gene26893.t1 |
| chr1.4 | exon | 80279952 | 80280358 | 80279952 | ID=MS.gene26893.t1.exon2;Parent=MS.gene26893.t1 |
| chr1.4 | CDS | 80279952 | 80280358 | 80279952 | ID=cds.MS.gene26893.t1;Parent=MS.gene26893.t1 |
| chr1.4 | exon | 80280494 | 80280631 | 80280494 | ID=MS.gene26893.t1.exon3;Parent=MS.gene26893.t1 |
| chr1.4 | CDS | 80280494 | 80280631 | 80280494 | ID=cds.MS.gene26893.t1;Parent=MS.gene26893.t1 |
| Gene Sequence |
| Protein sequence |
