AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27737

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27737.t1 MTR_7g077340 90.000 120 10 2 1 118 1 120 7.16e-74 215
MS.gene27737.t1 MTR_7g077215 80.808 99 17 1 13 111 14 110 4.13e-52 160
MS.gene27737.t1 MTR_7g077210 65.000 120 29 6 1 111 1 116 1.28e-42 136
MS.gene27737.t1 MTR_7g077180 62.162 111 17 2 1 111 1 86 4.54e-38 124
MS.gene27737.t1 MTR_7g077310 58.654 104 39 2 11 111 1 103 3.74e-37 122
MS.gene27737.t1 MTR_7g077220 64.583 96 22 1 10 105 1 84 1.20e-35 119
MS.gene27737.t1 MTR_7g077260 53.509 114 49 3 1 111 1 113 2.01e-34 115
MS.gene27737.t1 MTR_7g077300 54.622 119 43 4 1 118 1 109 1.22e-33 113
MS.gene27737.t1 MTR_7g077160 56.481 108 42 1 11 118 5 107 2.71e-30 104
MS.gene27737.t1 MTR_7g077350 50.877 114 46 5 6 112 23 133 4.57e-22 84.7
MS.gene27737.t1 MTR_7g077313 46.729 107 48 4 4 108 3 102 4.73e-22 83.6
MS.gene27737.t1 MTR_7g077190 55.000 60 27 0 54 113 22 81 1.27e-16 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTGTCACTGTGCTGATAT+TGG 0.334596 7.2:+28568795 MS.gene27737:CDS
AATGATGAAGTTAGCTTTGT+TGG 0.339085 7.2:+28568614 MS.gene27737:CDS
CAGTTGAAGTGAAGCCCAAA+AGG 0.468624 7.2:-28568641 None:intergenic
AACTTAGTGAGATCCACCTT+TGG 0.468934 7.2:-28568925 None:intergenic
CCACCTTTGGAGTAGCTACA+TGG 0.481606 7.2:-28568912 None:intergenic
ATCACAGCATGCTGTTGTTG+TGG 0.550411 7.2:-28568742 None:intergenic
ATCACTCAGCTCCTCTCCAA+TGG 0.555707 7.2:+28568696 MS.gene27737:CDS
ATCGTAAGTAGCTTCACCAT+TGG 0.558223 7.2:-28568712 None:intergenic
GGAGAGGAGCTGAGTGATGA+AGG 0.560141 7.2:-28568691 None:intergenic
CCATGTAGCTACTCCAAAGG+TGG 0.604280 7.2:+28568912 MS.gene27737:CDS
CAGCACAGTGACACTGAGGA+GGG 0.627236 7.2:-28568788 None:intergenic
GAGGAAAAGATCTTGTGCAA+AGG 0.628614 7.2:-28568848 None:intergenic
TCAGCACAGTGACACTGAGG+AGG 0.630876 7.2:-28568789 None:intergenic
TCAGTGCAGCGACACTGTGG+AGG 0.646863 7.2:-28568867 None:intergenic
GAACCATGTAGCTACTCCAA+AGG 0.655168 7.2:+28568909 MS.gene27737:CDS
AAGTAGCTTCACCATTGGAG+AGG 0.659642 7.2:-28568707 None:intergenic
TGGTTCATAGCAGAAGTCAG+TGG 0.693755 7.2:-28568892 None:intergenic
GTATCAGTGCAGCGACACTG+TGG 0.751185 7.2:-28568870 None:intergenic
ATATCAGCACAGTGACACTG+AGG 0.763170 7.2:-28568792 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGATGAAGTTAGCTTTGT+TGG + chr7.2:28568614-28568633 MS.gene27737:CDS 30.0%
!!! AGCTTTGTTGGTTTTCCTTT+TGG + chr7.2:28568626-28568645 MS.gene27737:CDS 35.0%
!!! GCTTTGTTGGTTTTCCTTTT+GGG + chr7.2:28568627-28568646 MS.gene27737:CDS 35.0%
ATCGTAAGTAGCTTCACCAT+TGG - chr7.2:28568715-28568734 None:intergenic 40.0%
GAGGAAAAGATCTTGTGCAA+AGG - chr7.2:28568851-28568870 None:intergenic 40.0%
!! GAGGGATTGATTTTGTGCAA+AGG - chr7.2:28568773-28568792 None:intergenic 40.0%
AAGTAGCTTCACCATTGGAG+AGG - chr7.2:28568710-28568729 None:intergenic 45.0%
ATATCAGCACAGTGACACTG+AGG - chr7.2:28568795-28568814 None:intergenic 45.0%
ATCACAGCATGCTGTTGTTG+TGG - chr7.2:28568745-28568764 None:intergenic 45.0%
CAGTTGAAGTGAAGCCCAAA+AGG - chr7.2:28568644-28568663 None:intergenic 45.0%
GAACCATGTAGCTACTCCAA+AGG + chr7.2:28568909-28568928 MS.gene27737:CDS 45.0%
TGGTTCATAGCAGAAGTCAG+TGG - chr7.2:28568895-28568914 None:intergenic 45.0%
! CAGTGTCACTGTGCTGATAT+TGG + chr7.2:28568795-28568814 MS.gene27737:CDS 45.0%
ATCACTCAGCTCCTCTCCAA+TGG + chr7.2:28568696-28568715 MS.gene27737:CDS 50.0%
CCATGTAGCTACTCCAAAGG+TGG + chr7.2:28568912-28568931 MS.gene27737:CDS 50.0%
!! CCACCTTTGGAGTAGCTACA+TGG - chr7.2:28568915-28568934 None:intergenic 50.0%
CAGCACAGTGACACTGAGGA+GGG - chr7.2:28568791-28568810 None:intergenic 55.0%
GGAGAGGAGCTGAGTGATGA+AGG - chr7.2:28568694-28568713 None:intergenic 55.0%
GTATCAGTGCAGCGACACTG+TGG - chr7.2:28568873-28568892 None:intergenic 55.0%
TCAGCACAGTGACACTGAGG+AGG - chr7.2:28568792-28568811 None:intergenic 55.0%
TCAGTGCAGCGACACTGTGG+AGG - chr7.2:28568870-28568889 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 28568588 28568944 28568588 ID=MS.gene27737
chr7.2 mRNA 28568588 28568944 28568588 ID=MS.gene27737.t1;Parent=MS.gene27737
chr7.2 exon 28568588 28568944 28568588 ID=MS.gene27737.t1.exon1;Parent=MS.gene27737.t1
chr7.2 CDS 28568588 28568944 28568588 ID=cds.MS.gene27737.t1;Parent=MS.gene27737.t1
Gene Sequence

>MS.gene27737

ATGGAGTTAATCATGAACAAGAATGCAATGATGAAGTTAGCTTTGTTGGTTTTCCTTTTGGGCTTCACTTCAACTGTTGTTGATGCTCGTTTCGATCGAGCATCCTTCATCACTCAGCTCCTCTCCAATGGTGAAGCTACTTACGATGTCAAATCCACAACAACAGCATGCTGTGATTTATGCCTTTGCACAAAATCAATCCCTCCTCAGTGTCACTGTGCTGATATTGGAGAAAAATGTCACTCAGCTTGCAAAAGATGCCTTTGCACAAGATCTTTTCCTCCACAGTGTCGCTGCACTGATACCACTGACTTCTGCTATGAACCATGTAGCTACTCCAAAGGTGGATCTCACTAA
Protein sequence

>MS.gene27737.t1

MELIMNKNAMMKLALLVFLLGFTSTVVDARFDRASFITQLLSNGEATYDVKSTTTACCDLCLCTKSIPPQCHCADIGEKCHSACKRCLCTRSFPPQCRCTDTTDFCYEPCSYSKGGSH