Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27763.t1 | XP_003623967.1 | 95.6 | 137 | 6 | 0 | 4 | 140 | 776 | 912 | 2.90E-71 | 277.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27763.t1 | Q2HTJ8 | 95.6 | 137 | 6 | 0 | 4 | 140 | 776 | 912 | 2.1e-71 | 277.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051744 | MS.gene27763 | 0.801303 | 9.59E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene27763 | 0.808204 | 3.47E-50 | -1.69E-46 |
| MS.gene060962 | MS.gene27763 | 0.805756 | 1.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27763.t1 | MTR_7g077740 | 95.620 | 137 | 6 | 0 | 4 | 140 | 776 | 912 | 3.76e-90 | 280 |
| MS.gene27763.t1 | MTR_8g030750 | 69.403 | 134 | 41 | 0 | 4 | 137 | 723 | 856 | 2.58e-62 | 204 |
| MS.gene27763.t1 | MTR_7g078520 | 91.667 | 60 | 5 | 0 | 74 | 133 | 17 | 76 | 7.52e-34 | 115 |
| MS.gene27763.t1 | MTR_2g096870 | 27.338 | 139 | 90 | 3 | 5 | 132 | 554 | 692 | 2.28e-12 | 63.5 |
| MS.gene27763.t1 | MTR_2g096870 | 27.338 | 139 | 90 | 3 | 5 | 132 | 592 | 730 | 2.48e-12 | 63.2 |
| MS.gene27763.t1 | MTR_7g076780 | 80.645 | 31 | 6 | 0 | 18 | 48 | 36 | 66 | 3.42e-11 | 55.5 |
| MS.gene27763.t1 | MTR_1g047660 | 30.435 | 138 | 83 | 4 | 8 | 132 | 710 | 847 | 6.04e-11 | 59.3 |
| MS.gene27763.t1 | MTR_8g098335 | 30.370 | 135 | 78 | 4 | 11 | 140 | 449 | 572 | 7.24e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27763.t1 | AT2G45900 | 51.562 | 128 | 58 | 1 | 3 | 130 | 580 | 703 | 2.08e-37 | 134 |
| MS.gene27763.t1 | AT2G45900 | 51.562 | 128 | 58 | 1 | 3 | 130 | 580 | 703 | 2.08e-37 | 134 |
| MS.gene27763.t1 | AT2G45900 | 51.562 | 128 | 58 | 1 | 3 | 130 | 580 | 703 | 2.08e-37 | 134 |
| MS.gene27763.t1 | AT3G61380 | 46.154 | 130 | 70 | 0 | 3 | 132 | 571 | 700 | 6.96e-35 | 127 |
| MS.gene27763.t1 | AT3G61380 | 46.154 | 130 | 70 | 0 | 3 | 132 | 571 | 700 | 6.96e-35 | 127 |
| MS.gene27763.t1 | AT3G61380 | 46.154 | 130 | 70 | 0 | 3 | 132 | 571 | 700 | 6.96e-35 | 127 |
| MS.gene27763.t1 | AT4G00440 | 49.138 | 116 | 55 | 1 | 3 | 118 | 683 | 794 | 2.12e-27 | 106 |
| MS.gene27763.t1 | AT4G00440 | 49.138 | 116 | 55 | 1 | 3 | 118 | 683 | 794 | 2.12e-27 | 106 |
| MS.gene27763.t1 | AT4G00440 | 49.138 | 116 | 55 | 1 | 3 | 118 | 683 | 794 | 2.16e-27 | 106 |
| MS.gene27763.t1 | AT4G00440 | 49.138 | 116 | 55 | 1 | 3 | 118 | 683 | 794 | 2.20e-27 | 106 |
| MS.gene27763.t1 | AT4G00440 | 49.138 | 116 | 55 | 1 | 3 | 118 | 683 | 794 | 2.20e-27 | 106 |
| MS.gene27763.t1 | AT1G01695 | 39.496 | 119 | 67 | 2 | 4 | 121 | 300 | 414 | 2.61e-22 | 91.3 |
Find 38 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTTTGTTGGCATTACTTT+GGG | 0.241383 | 7.3:-29093985 | MS.gene27763:CDS |
| GCCAACTCCAAATATGAAAA+AGG | 0.305070 | 7.3:-29093924 | MS.gene27763:CDS |
| GAGGTTTGTTGGCATTACTT+TGG | 0.368132 | 7.3:-29093986 | MS.gene27763:CDS |
| TATTGATGAAATGTCTTTCT+CGG | 0.369351 | 7.3:-29094113 | None:intergenic |
| ATTACTTTGGGGTCTCGCCT+TGG | 0.381794 | 7.3:-29093973 | MS.gene27763:CDS |
| TTACTTTGGGGTCTCGCCTT+GGG | 0.384536 | 7.3:-29093972 | MS.gene27763:CDS |
| AAAAGGTTATTCTCAAGGTC+TGG | 0.384863 | 7.3:-29093907 | MS.gene27763:CDS |
| CGAATAGGAGCTTCTGTTCA+TGG | 0.386503 | 7.3:+29094032 | None:intergenic |
| AGATCCTTCGTTGTTTGATC+AGG | 0.424810 | 7.3:-29094080 | MS.gene27763:CDS |
| CCTCGTTGATACAGTCGAAT+AGG | 0.429863 | 7.3:+29094017 | None:intergenic |
| CATTTGTAAACTCTAGTATA+AGG | 0.433003 | 7.3:-29093946 | MS.gene27763:CDS |
| CGACTCGATGCTGAGATAGT+TGG | 0.454596 | 7.3:-29093785 | MS.gene27763:CDS |
| TTCCATCAGTTCTGCAAGAA+TGG | 0.457038 | 7.3:+29093745 | None:intergenic |
| AGGTTCTCATGGAGGTTTGT+TGG | 0.459209 | 7.3:-29093997 | MS.gene27763:CDS |
| TTTGCCTCCACCGCACACTT+TGG | 0.460330 | 7.3:-29093858 | MS.gene27763:CDS |
| TGTTCCAAAGTGTGCGGTGG+AGG | 0.469771 | 7.3:+29093854 | None:intergenic |
| CTGTATCAACGAGGTTCTCA+TGG | 0.487452 | 7.3:-29094008 | MS.gene27763:CDS |
| CACCATTCTTGCAGAACTGA+TGG | 0.495569 | 7.3:-29093747 | MS.gene27763:CDS |
| AAAGGTTATTCTCAAGGTCT+GGG | 0.495861 | 7.3:-29093906 | MS.gene27763:CDS |
| CACACTTTGGAACAAATTGT+CGG | 0.507212 | 7.3:-29093845 | MS.gene27763:CDS |
| AGGTCTGGGAAGGAGTTTGT+TGG | 0.511200 | 7.3:-29093892 | MS.gene27763:CDS |
| GGAAAAGACATGGCGAGGAA+TGG | 0.538407 | 7.3:-29093824 | MS.gene27763:CDS |
| GGTTTGTTGGCATTACTTTG+GGG | 0.549876 | 7.3:-29093984 | MS.gene27763:CDS |
| AAAGTGTGCGGTGGAGGCAA+AGG | 0.555155 | 7.3:+29093860 | None:intergenic |
| CTATCTCAGCATCGAGTCGA+AGG | 0.558438 | 7.3:+29093789 | None:intergenic |
| GAGTCTTGTGAGTCAAAGTA+CGG | 0.578745 | 7.3:-29093711 | MS.gene27763:CDS |
| GGCGAGGAATGGAACATGGA+TGG | 0.579182 | 7.3:-29093813 | MS.gene27763:CDS |
| ACATGGCGAGGAATGGAACA+TGG | 0.601786 | 7.3:-29093817 | MS.gene27763:CDS |
| ACAAATTGTCGGAAAAGACA+TGG | 0.608217 | 7.3:-29093834 | MS.gene27763:CDS |
| ATTTGTTCCAAAGTGTGCGG+TGG | 0.613422 | 7.3:+29093851 | None:intergenic |
| TTGTCGGAAAAGACATGGCG+AGG | 0.616578 | 7.3:-29093829 | MS.gene27763:CDS |
| GTTATTCTCAAGGTCTGGGA+AGG | 0.618775 | 7.3:-29093902 | MS.gene27763:CDS |
| TGTGCGGTGGAGGCAAAGGA+AGG | 0.625762 | 7.3:+29093864 | None:intergenic |
| ACAATTTGTTCCAAAGTGTG+CGG | 0.645595 | 7.3:+29093848 | None:intergenic |
| TCTACCTGATCAAACAACGA+AGG | 0.666820 | 7.3:+29094076 | None:intergenic |
| GAGTTTACAAATGATACCCA+AGG | 0.668169 | 7.3:+29093956 | None:intergenic |
| TATCAACGAGGTTCTCATGG+AGG | 0.685357 | 7.3:-29094005 | MS.gene27763:CDS |
| CCTATTCGACTGTATCAACG+AGG | 0.741358 | 7.3:-29094017 | MS.gene27763:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAATAACCTTTTTCATATT+TGG | + | chr7.3:29093892-29093911 | None:intergenic | 20.0% |
| ! | CATTTGTAAACTCTAGTATA+AGG | - | chr7.3:29093860-29093879 | MS.gene27763:CDS | 25.0% |
| ! | TATGAAAAAGGTTATTCTCA+AGG | - | chr7.3:29093894-29093913 | MS.gene27763:CDS | 25.0% |
| !! | ACCTTTTTCATATTTGGAGT+TGG | + | chr7.3:29093886-29093905 | None:intergenic | 30.0% |
| !!! | GAGATAGTTGGTTTTGAAAT+GGG | - | chr7.3:29094033-29094052 | MS.gene27763:CDS | 30.0% |
| !!! | TGAGATAGTTGGTTTTGAAA+TGG | - | chr7.3:29094032-29094051 | MS.gene27763:CDS | 30.0% |
| AAAAGGTTATTCTCAAGGTC+TGG | - | chr7.3:29093899-29093918 | MS.gene27763:CDS | 35.0% | |
| AAAGGTTATTCTCAAGGTCT+GGG | - | chr7.3:29093900-29093919 | MS.gene27763:CDS | 35.0% | |
| ACAAATTGTCGGAAAAGACA+TGG | - | chr7.3:29093972-29093991 | MS.gene27763:CDS | 35.0% | |
| GAGTTTACAAATGATACCCA+AGG | + | chr7.3:29093853-29093872 | None:intergenic | 35.0% | |
| GCCAACTCCAAATATGAAAA+AGG | - | chr7.3:29093882-29093901 | MS.gene27763:CDS | 35.0% | |
| ! | ACAATTTGTTCCAAAGTGTG+CGG | + | chr7.3:29093961-29093980 | None:intergenic | 35.0% |
| !! | AGGTTTGTTGGCATTACTTT+GGG | - | chr7.3:29093821-29093840 | MS.gene27763:CDS | 35.0% |
| !! | CACACTTTGGAACAAATTGT+CGG | - | chr7.3:29093961-29093980 | MS.gene27763:CDS | 35.0% |
| AGATCCTTCGTTGTTTGATC+AGG | - | chr7.3:29093726-29093745 | MS.gene27763:CDS | 40.0% | |
| TCTACCTGATCAAACAACGA+AGG | + | chr7.3:29093733-29093752 | None:intergenic | 40.0% | |
| TTCCATCAGTTCTGCAAGAA+TGG | + | chr7.3:29094064-29094083 | None:intergenic | 40.0% | |
| ! | GAGTCTTGTGAGTCAAAGTA+CGG | - | chr7.3:29094095-29094114 | MS.gene27763:CDS | 40.0% |
| !! | GAGGTTTGTTGGCATTACTT+TGG | - | chr7.3:29093820-29093839 | MS.gene27763:CDS | 40.0% |
| !! | GGTTTGTTGGCATTACTTTG+GGG | - | chr7.3:29093822-29093841 | MS.gene27763:CDS | 40.0% |
| AGGTTCTCATGGAGGTTTGT+TGG | - | chr7.3:29093809-29093828 | MS.gene27763:CDS | 45.0% | |
| ATTTGTTCCAAAGTGTGCGG+TGG | + | chr7.3:29093958-29093977 | None:intergenic | 45.0% | |
| CACCATTCTTGCAGAACTGA+TGG | - | chr7.3:29094059-29094078 | MS.gene27763:CDS | 45.0% | |
| CCTATTCGACTGTATCAACG+AGG | - | chr7.3:29093789-29093808 | MS.gene27763:CDS | 45.0% | |
| CCTCGTTGATACAGTCGAAT+AGG | + | chr7.3:29093792-29093811 | None:intergenic | 45.0% | |
| CGAATAGGAGCTTCTGTTCA+TGG | + | chr7.3:29093777-29093796 | None:intergenic | 45.0% | |
| CTGTATCAACGAGGTTCTCA+TGG | - | chr7.3:29093798-29093817 | MS.gene27763:CDS | 45.0% | |
| GTTATTCTCAAGGTCTGGGA+AGG | - | chr7.3:29093904-29093923 | MS.gene27763:CDS | 45.0% | |
| TATCAACGAGGTTCTCATGG+AGG | - | chr7.3:29093801-29093820 | MS.gene27763:CDS | 45.0% | |
| ACATGGCGAGGAATGGAACA+TGG | - | chr7.3:29093989-29094008 | MS.gene27763:CDS | 50.0% | |
| AGGTCTGGGAAGGAGTTTGT+TGG | - | chr7.3:29093914-29093933 | MS.gene27763:CDS | 50.0% | |
| CGACTCGATGCTGAGATAGT+TGG | - | chr7.3:29094021-29094040 | MS.gene27763:CDS | 50.0% | |
| GGAAAAGACATGGCGAGGAA+TGG | - | chr7.3:29093982-29094001 | MS.gene27763:CDS | 50.0% | |
| TTGTCGGAAAAGACATGGCG+AGG | - | chr7.3:29093977-29093996 | MS.gene27763:CDS | 50.0% | |
| ! | CTATCTCAGCATCGAGTCGA+AGG | + | chr7.3:29094020-29094039 | None:intergenic | 50.0% |
| !! | ATTACTTTGGGGTCTCGCCT+TGG | - | chr7.3:29093833-29093852 | MS.gene27763:CDS | 50.0% |
| !! | TTACTTTGGGGTCTCGCCTT+GGG | - | chr7.3:29093834-29093853 | MS.gene27763:CDS | 50.0% |
| AAAGTGTGCGGTGGAGGCAA+AGG | + | chr7.3:29093949-29093968 | None:intergenic | 55.0% | |
| GGCGAGGAATGGAACATGGA+TGG | - | chr7.3:29093993-29094012 | MS.gene27763:CDS | 55.0% | |
| TGTTCCAAAGTGTGCGGTGG+AGG | + | chr7.3:29093955-29093974 | None:intergenic | 55.0% | |
| TTTGCCTCCACCGCACACTT+TGG | - | chr7.3:29093948-29093967 | MS.gene27763:CDS | 55.0% | |
| TGTGCGGTGGAGGCAAAGGA+AGG | + | chr7.3:29093945-29093964 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 29093703 | 29094125 | 29093703 | ID=MS.gene27763 |
| chr7.3 | mRNA | 29093703 | 29094125 | 29093703 | ID=MS.gene27763.t1;Parent=MS.gene27763 |
| chr7.3 | exon | 29093703 | 29094125 | 29093703 | ID=MS.gene27763.t1.exon1;Parent=MS.gene27763.t1 |
| chr7.3 | CDS | 29093703 | 29094125 | 29093703 | ID=cds.MS.gene27763.t1;Parent=MS.gene27763.t1 |
| Gene Sequence |
| Protein sequence |
