AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27777


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27777.t1 MTR_7g074820 94.118 187 11 0 1 187 143 329 2.36e-130 369
MS.gene27777.t1 MTR_7g074850 89.305 187 20 0 1 187 143 329 2.19e-123 351
MS.gene27777.t1 MTR_7g074730 88.235 187 21 1 1 187 143 328 1.16e-121 347
MS.gene27777.t1 MTR_7g074880 73.262 187 47 2 1 187 143 326 9.20e-101 294
MS.gene27777.t1 MTR_7g074870 71.123 187 51 2 1 187 105 288 1.53e-96 281
MS.gene27777.t1 MTR_7g074710 63.102 187 66 1 1 187 143 326 3.74e-83 249
MS.gene27777.t1 MTR_1g022440 43.229 192 96 3 1 187 142 325 1.94e-47 158
MS.gene27777.t1 MTR_1g022445 43.455 191 95 3 2 187 143 325 4.21e-46 154
MS.gene27777.t1 MTR_4g023730 43.617 188 103 2 1 187 144 329 6.27e-44 149
MS.gene27777.t1 MTR_4g092080 37.368 190 112 3 1 185 149 336 8.13e-39 135
MS.gene27777.t1 MTR_4g081440 37.306 193 112 4 1 187 165 354 4.26e-38 134
MS.gene27777.t1 MTR_4g081440 37.306 193 111 5 1 187 161 349 1.11e-36 130
MS.gene27777.t1 MTR_2g101330 36.898 187 109 6 1 187 142 319 8.20e-31 114
MS.gene27777.t1 MTR_5g056600 34.043 188 115 4 1 187 140 319 8.54e-29 109
MS.gene27777.t1 MTR_2g013230 33.816 207 105 7 1 187 56 250 7.44e-25 97.4
MS.gene27777.t1 MTR_2g013230 33.816 207 105 7 1 187 146 340 4.17e-24 97.1
MS.gene27777.t1 MTR_3g031650 33.158 190 109 6 3 187 143 319 2.35e-22 92.0
MS.gene27777.t1 MTR_2g013250 32.000 200 114 5 1 185 145 337 8.34e-21 88.2
MS.gene27777.t1 MTR_5g072620 33.158 190 109 5 3 187 143 319 8.62e-21 87.8
MS.gene27777.t1 MTR_3g005210 31.915 188 117 4 1 187 143 320 1.17e-20 87.4
MS.gene27777.t1 MTR_4g009690 31.383 188 115 5 1 187 148 322 5.89e-19 82.8
MS.gene27777.t1 MTR_3g005170 31.016 187 118 5 1 186 116 292 1.27e-18 81.6
MS.gene27777.t1 MTR_8g062110 31.522 184 116 4 1 184 60 233 1.29e-18 80.5
MS.gene27777.t1 MTR_4g009040 30.851 188 117 5 1 187 152 327 1.60e-18 81.6
MS.gene27777.t1 MTR_4g009040 30.851 188 117 5 1 187 148 323 1.70e-18 81.6
MS.gene27777.t1 MTR_3g005170 31.016 187 118 5 1 186 147 323 2.45e-18 81.3
MS.gene27777.t1 MTR_4g009690 30.688 189 116 5 1 187 148 323 3.48e-18 80.9
MS.gene27777.t1 MTR_2g028620 31.122 196 110 7 1 187 147 326 4.27e-18 80.5
MS.gene27777.t1 MTR_8g062110 31.522 184 116 4 1 184 155 328 5.38e-18 80.5
MS.gene27777.t1 MTR_8g062110 31.522 184 116 4 1 184 146 319 5.66e-18 80.1
MS.gene27777.t1 MTR_3g005170 34.058 138 84 3 1 138 147 277 2.69e-17 77.8
MS.gene27777.t1 MTR_4g077130 35.252 139 81 5 1 138 146 276 4.62e-17 77.8
MS.gene27777.t1 MTR_7g117190 31.000 200 121 8 1 187 35 230 5.94e-17 75.9
MS.gene27777.t1 MTR_2g013260 33.659 205 104 8 1 185 75 267 1.17e-16 75.9
MS.gene27777.t1 MTR_3g031650 33.333 135 84 2 3 137 143 271 1.65e-16 75.5
MS.gene27777.t1 MTR_8g062110 33.577 137 83 2 1 137 60 188 1.78e-16 73.9
MS.gene27777.t1 MTR_2g013260 33.659 205 104 8 1 185 146 338 2.59e-16 75.9
MS.gene27777.t1 MTR_7g117190 31.000 200 121 8 1 187 145 340 2.63e-16 75.9
MS.gene27777.t1 MTR_4g006940 29.630 189 122 5 1 187 147 326 3.45e-16 75.5
MS.gene27777.t1 MTR_2g104960 31.217 189 119 6 1 187 150 329 3.77e-16 75.5
MS.gene27777.t1 MTR_8g062110 33.577 137 83 2 1 137 146 274 7.31e-16 73.9
MS.gene27777.t1 MTR_8g062110 33.103 145 88 3 1 145 146 281 7.50e-16 73.9
MS.gene27777.t1 MTR_0685s0030 35.593 118 70 3 22 138 3 115 2.97e-15 68.9
MS.gene27777.t1 MTR_2g028620 34.586 133 80 3 1 132 147 273 3.31e-15 72.4
MS.gene27777.t1 MTR_0685s0030 35.593 118 70 3 22 138 3 115 8.27e-15 68.9
MS.gene27777.t1 MTR_8g062440 29.787 188 120 5 1 187 145 321 2.87e-14 69.7
MS.gene27777.t1 MTR_8g062440 29.787 188 120 5 1 187 146 322 3.13e-14 69.7
MS.gene27777.t1 MTR_4g006940 31.655 139 89 2 1 139 147 279 2.23e-13 67.0
MS.gene27777.t1 MTR_4g006940 31.655 139 89 2 1 139 147 279 2.23e-13 67.0
MS.gene27777.t1 MTR_8g062440 33.333 135 82 3 1 135 145 271 2.10e-12 64.7
MS.gene27777.t1 MTR_4g077100 26.812 138 94 2 1 138 148 278 2.96e-11 61.2
MS.gene27777.t1 MTR_4g077100 26.812 138 94 2 1 138 148 278 3.28e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27777.t1 AT2G45400 49.735 189 90 3 1 187 122 307 5.38e-52 169
MS.gene27777.t1 AT2G45400 49.735 189 90 3 1 187 179 364 1.52e-51 169
MS.gene27777.t1 AT5G42800 46.032 189 93 4 2 187 143 325 1.22e-45 154
MS.gene27777.t1 AT2G45400 51.064 141 65 2 1 139 179 317 2.11e-39 137
MS.gene27777.t1 AT4G35420 40.107 187 103 5 1 187 144 321 5.15e-38 133
MS.gene27777.t1 AT1G61720 36.458 192 115 3 1 187 148 337 1.30e-33 122
MS.gene27777.t1 AT1G68540 36.170 188 111 4 1 187 35 214 3.37e-29 107
MS.gene27777.t1 AT1G68540 36.170 188 111 4 1 187 142 321 2.53e-28 108
MS.gene27777.t1 AT2G02400 35.233 193 105 6 1 187 140 318 2.61e-28 107
MS.gene27777.t1 AT1G25460 34.574 188 114 4 1 187 140 319 3.30e-27 105
MS.gene27777.t1 AT5G58490 36.649 191 103 6 2 187 146 323 5.69e-24 96.7
MS.gene27777.t1 AT4G27250 30.435 184 123 2 1 179 155 338 3.03e-22 92.4
MS.gene27777.t1 AT4G27250 29.508 183 116 2 1 179 155 328 8.26e-22 90.9
MS.gene27777.t1 AT1G51410 30.319 188 120 5 1 187 146 323 4.83e-20 85.9
MS.gene27777.t1 AT1G76470 30.508 177 117 3 2 178 143 313 1.57e-19 84.3
MS.gene27777.t1 AT2G45400 56.579 76 31 1 1 74 179 254 6.08e-19 82.0
MS.gene27777.t1 AT1G09480 34.043 188 113 6 1 187 145 322 1.68e-18 81.6
MS.gene27777.t1 AT5G19440 32.620 187 115 4 1 186 147 323 2.56e-18 81.3
MS.gene27777.t1 AT1G09480 34.574 188 112 7 1 187 192 369 2.83e-18 81.6
MS.gene27777.t1 AT1G09490 34.507 142 86 4 1 142 114 248 5.55e-17 77.0
MS.gene27777.t1 AT1G09490 33.803 142 87 3 1 142 145 279 8.96e-17 77.0
MS.gene27777.t1 AT1G15950 30.159 189 121 5 1 187 45 224 3.09e-16 74.3
MS.gene27777.t1 AT1G15950 30.159 189 121 5 1 187 143 322 1.11e-15 73.9
MS.gene27777.t1 AT1G15950 30.159 189 121 5 1 187 143 322 1.11e-15 73.9
MS.gene27777.t1 AT1G09510 33.155 187 110 6 1 184 145 319 1.44e-15 73.6
MS.gene27777.t1 AT1G09500 32.394 142 89 4 1 142 112 246 1.71e-13 67.4
MS.gene27777.t1 AT1G15950 29.143 175 118 2 1 175 143 311 1.92e-13 67.8
MS.gene27777.t1 AT2G33600 28.415 183 113 6 1 179 144 312 2.26e-13 67.4
MS.gene27777.t1 AT1G09500 31.690 142 90 3 1 142 146 280 2.35e-13 67.4
MS.gene27777.t1 AT1G09500 32.593 135 84 3 1 135 146 273 3.06e-13 66.6
MS.gene27777.t1 AT1G66800 34.074 135 82 4 1 135 141 268 1.59e-12 65.1
MS.gene27777.t1 AT1G80820 28.947 190 122 6 1 187 138 317 2.79e-12 64.3
MS.gene27777.t1 AT1G15950 32.143 140 89 2 1 140 143 276 4.14e-12 63.5
MS.gene27777.t1 AT1G15950 32.143 140 89 2 1 140 143 276 4.92e-12 63.2
MS.gene27777.t1 AT1G15950 32.374 139 88 2 1 139 143 275 5.22e-12 63.2

Find 38 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCACTAGCTTTCTTATT+TGG 0.183198 7.3:-30578077 MS.gene27777:intron
GAGAAAGCTGTTCTAGAATT+TGG 0.187155 7.3:-30578188 MS.gene27777:CDS
TTCATCTTCTAGAGAGCTTA+TGG 0.209568 7.3:-30577656 MS.gene27777:intron
GAGCAATTATATCTTCCTTT+TGG 0.266827 7.3:+30577837 None:intergenic
AGAGAGCTTATGGGAGTTAA+AGG 0.273617 7.3:-30577646 MS.gene27777:CDS
GATGTTGGCATTTGAAGTTC+AGG 0.351951 7.3:+30577762 None:intergenic
AGGTAATGGATGAAAGTTAT+TGG 0.353449 7.3:-30578280 None:intergenic
CATTTGAAGTTCAGGATACT+TGG 0.362756 7.3:+30577770 None:intergenic
ATTTGGAGAACAACATGGTT+TGG 0.364972 7.3:-30578171 MS.gene27777:CDS
AATTGAATGTTGCAAGGAAA+AGG 0.371801 7.3:-30577536 MS.gene27777:CDS
ATAAGAAAGCTAGTGAACTA+TGG 0.381083 7.3:+30578081 None:intergenic
GAGGTTAAATGTGGAAACTT+AGG 0.398400 7.3:+30577622 None:intergenic
TCATCTTCTAGAGAGCTTAT+GGG 0.409352 7.3:-30577655 MS.gene27777:intron
TCTTCAATAGTTGCAATGAA+TGG 0.434791 7.3:+30577813 None:intergenic
ATCATCCACGAGTTTCTTTG+AGG 0.447908 7.3:+30577603 None:intergenic
GTACGAGATACAAGCACTAA+TGG 0.458074 7.3:+30577921 None:intergenic
TTCATTTGTCCTAAGCTACC+TGG 0.469552 7.3:-30578110 MS.gene27777:CDS
CGTAGAACTCACTTGGATGT+TGG 0.480075 7.3:+30577747 None:intergenic
TCAAAGAAACTCGTGGATGA+TGG 0.487540 7.3:-30577601 MS.gene27777:CDS
CTTGAACATCCTGATCCAAA+AGG 0.491543 7.3:-30577852 MS.gene27777:CDS
ATTGAATGTTGCAAGGAAAA+GGG 0.495429 7.3:-30577535 MS.gene27777:CDS
TGGACAGATCCAGGTAGCTT+AGG 0.512154 7.3:+30578101 None:intergenic
ACAGCATATGACCAAGCAAA+AGG 0.516501 7.3:+30578227 None:intergenic
TAGCCTTGAGGAAATGTTTG+AGG 0.521228 7.3:-30577563 MS.gene27777:CDS
AAAGGTCCAACAACAAAAGT+TGG 0.534220 7.3:+30578131 None:intergenic
GCTTGTATCTCGTACCGGTA+TGG 0.538328 7.3:-30577913 MS.gene27777:CDS
AGTGAACTATGGACAGATCC+AGG 0.545594 7.3:+30578092 None:intergenic
ATCATCAACATGCACCATAC+CGG 0.559899 7.3:+30577899 None:intergenic
AGTTTCTTTGAGGTTAAATG+TGG 0.564214 7.3:+30577613 None:intergenic
CTAGAATTTGGAGAACAACA+TGG 0.590067 7.3:-30578176 MS.gene27777:CDS
CGTCCTCAAACATTTCCTCA+AGG 0.591440 7.3:+30577560 None:intergenic
CAGGATACTTGGAAGAAACA+AGG 0.598723 7.3:+30577781 None:intergenic
TTAGTGCTTGTATCTCGTAC+CGG 0.600363 7.3:-30577918 MS.gene27777:CDS
GGACGCAATTGAATGTTGCA+AGG 0.609289 7.3:-30577542 MS.gene27777:CDS
TTTAACCTCAAAGAAACTCG+TGG 0.633314 7.3:-30577608 MS.gene27777:CDS
TATGGTGCATGTTGATGATG+TGG 0.643007 7.3:-30577895 MS.gene27777:CDS
GGTAGCTTAGGACAAATGAA+AGG 0.676303 7.3:+30578113 None:intergenic
CATGTTCAAGTATAGCCTTG+AGG 0.686468 7.3:-30577575 MS.gene27777:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGATAAAAATTAACAA+AGG - chr7.3:30577826-30577845 MS.gene27777:CDS 10.0%
AATTGAATGTTGCAAGGAAA+AGG - chr7.3:30578264-30578283 MS.gene27777:CDS 30.0%
ATAAGAAAGCTAGTGAACTA+TGG + chr7.3:30577722-30577741 None:intergenic 30.0%
ATTATTTCGTAGAACTCACT+TGG + chr7.3:30578063-30578082 None:intergenic 30.0%
ATTGAATGTTGCAAGGAAAA+GGG - chr7.3:30578265-30578284 MS.gene27777:CDS 30.0%
TCTTCAATAGTTGCAATGAA+TGG + chr7.3:30577990-30578009 None:intergenic 30.0%
! GAGCAATTATATCTTCCTTT+TGG + chr7.3:30577966-30577985 None:intergenic 30.0%
! TAACAAAGGAATTTTCATGC+AGG - chr7.3:30577840-30577859 MS.gene27777:CDS 30.0%
! TTATATCTTCCTTTTGGATC+AGG + chr7.3:30577960-30577979 None:intergenic 30.0%
!! AGTTCACTAGCTTTCTTATT+TGG - chr7.3:30577723-30577742 MS.gene27777:intron 30.0%
!! AGTTTCTTTGAGGTTAAATG+TGG + chr7.3:30578190-30578209 None:intergenic 30.0%
!! CTAATACCAACTTTTGTTGT+TGG - chr7.3:30577663-30577682 MS.gene27777:intron 30.0%
AAAGGTCCAACAACAAAAGT+TGG + chr7.3:30577672-30577691 None:intergenic 35.0%
ATTTGGAGAACAACATGGTT+TGG - chr7.3:30577629-30577648 MS.gene27777:CDS 35.0%
CTAGAATTTGGAGAACAACA+TGG - chr7.3:30577624-30577643 MS.gene27777:CDS 35.0%
CTTCTTACCATGTTTCTCAT+TGG - chr7.3:30577758-30577777 MS.gene27777:intron 35.0%
GAGGTTAAATGTGGAAACTT+AGG + chr7.3:30578181-30578200 None:intergenic 35.0%
TTTAACCTCAAAGAAACTCG+TGG - chr7.3:30578192-30578211 MS.gene27777:CDS 35.0%
! CATTTGAAGTTCAGGATACT+TGG + chr7.3:30578033-30578052 None:intergenic 35.0%
! TCATCTTCTAGAGAGCTTAT+GGG - chr7.3:30578145-30578164 MS.gene27777:CDS 35.0%
! TTCATCTTCTAGAGAGCTTA+TGG - chr7.3:30578144-30578163 MS.gene27777:CDS 35.0%
!! GAAATTTGAAGCCTTTTGCT+TGG - chr7.3:30577562-30577581 MS.gene27777:CDS 35.0%
!! GAGAAAGCTGTTCTAGAATT+TGG - chr7.3:30577612-30577631 MS.gene27777:CDS 35.0%
ACAGCATATGACCAAGCAAA+AGG + chr7.3:30577576-30577595 None:intergenic 40.0%
AGAGAGCTTATGGGAGTTAA+AGG - chr7.3:30578154-30578173 MS.gene27777:CDS 40.0%
ATCATCAACATGCACCATAC+CGG + chr7.3:30577904-30577923 None:intergenic 40.0%
ATCATCCACGAGTTTCTTTG+AGG + chr7.3:30578200-30578219 None:intergenic 40.0%
CAGGATACTTGGAAGAAACA+AGG + chr7.3:30578022-30578041 None:intergenic 40.0%
CCAAGAACCAATGAGAAACA+TGG + chr7.3:30577768-30577787 None:intergenic 40.0%
CTTGAACATCCTGATCCAAA+AGG - chr7.3:30577948-30577967 MS.gene27777:intron 40.0%
GGTAGCTTAGGACAAATGAA+AGG + chr7.3:30577690-30577709 None:intergenic 40.0%
GTACGAGATACAAGCACTAA+TGG + chr7.3:30577882-30577901 None:intergenic 40.0%
TCAAAGAAACTCGTGGATGA+TGG - chr7.3:30578199-30578218 MS.gene27777:CDS 40.0%
TTAGTGCTTGTATCTCGTAC+CGG - chr7.3:30577882-30577901 MS.gene27777:CDS 40.0%
TTCATTTGTCCTAAGCTACC+TGG - chr7.3:30577690-30577709 MS.gene27777:intron 40.0%
! CATGTTCAAGTATAGCCTTG+AGG - chr7.3:30578225-30578244 MS.gene27777:CDS 40.0%
! GATGTTGGCATTTGAAGTTC+AGG + chr7.3:30578041-30578060 None:intergenic 40.0%
! TAGCCTTGAGGAAATGTTTG+AGG - chr7.3:30578237-30578256 MS.gene27777:CDS 40.0%
!! CCATGTTTCTCATTGGTTCT+TGG - chr7.3:30577765-30577784 MS.gene27777:CDS 40.0%
!! TATGGTGCATGTTGATGATG+TGG - chr7.3:30577905-30577924 MS.gene27777:CDS 40.0%
AGTGAACTATGGACAGATCC+AGG + chr7.3:30577711-30577730 None:intergenic 45.0%
CGTAGAACTCACTTGGATGT+TGG + chr7.3:30578056-30578075 None:intergenic 45.0%
CGTCCTCAAACATTTCCTCA+AGG + chr7.3:30578243-30578262 None:intergenic 45.0%
GGACGCAATTGAATGTTGCA+AGG - chr7.3:30578258-30578277 MS.gene27777:CDS 45.0%
TGGACAGATCCAGGTAGCTT+AGG + chr7.3:30577702-30577721 None:intergenic 50.0%
! GCTTGTATCTCGTACCGGTA+TGG - chr7.3:30577887-30577906 MS.gene27777:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 30577525 30578297 30577525 ID=MS.gene27777
chr7.3 mRNA 30577525 30578297 30577525 ID=MS.gene27777.t1;Parent=MS.gene27777
chr7.3 exon 30578078 30578297 30578078 ID=MS.gene27777.t1.exon1;Parent=MS.gene27777.t1
chr7.3 CDS 30578078 30578297 30578078 ID=cds.MS.gene27777.t1;Parent=MS.gene27777.t1
chr7.3 exon 30577759 30577960 30577759 ID=MS.gene27777.t1.exon2;Parent=MS.gene27777.t1
chr7.3 CDS 30577759 30577960 30577759 ID=cds.MS.gene27777.t1;Parent=MS.gene27777.t1
chr7.3 exon 30577525 30577666 30577525 ID=MS.gene27777.t1.exon3;Parent=MS.gene27777.t1
chr7.3 CDS 30577525 30577666 30577525 ID=cds.MS.gene27777.t1;Parent=MS.gene27777.t1
Gene Sequence

>MS.gene27777

ATGGATGAAAGTTATTGGAGTGATGTGAATATTCTTAGAAATTTGAAGCCTTTTGCTTGGTCATATGCTGTTTCTAAGACACTAGCTGAGAAAGCTGTTCTAGAATTTGGAGAACAACATGGTTTGGATATCGTGACTCTAATACCAACTTTTGTTGTTGGACCTTTCATTTGTCCTAAGCTACCTGGATCTGTCCATAGTTCACTAGCTTTCTTATTTGGTAAGTGAAGTTTCTTCTTACCATGTTTCTCATTGGTTCTTGGACTATTGTGAAAGTATTTTCTTGATTATTTTATTTCTTAAATTGATAAAAATTAACAAAGGAATTTTCATGCAGGTGATATTGACAAGAATCCATTAGTGCTTGTATCTCGTACCGGTATGGTGCATGTTGATGATGTGGCTAGAGCACATATATTCTTACTTGAACATCCTGATCCAAAAGGAAGATATAATTGCTCACCATTCATTGCAACTATTGAAGAAATAGTTGACCTTGTTTCTTCCAAGTATCCTGAACTTCAAATGCCAACATCCAAGTGAGTTCTACGAAATAATTAGTTACATTAATTTGTGTGAGCTTTCTTTTGTTTTTACTAATAATATTATTCAATGTATTTTCATCTTCTAGAGAGCTTATGGGAGTTAAAGGTCCTAAGTTTCCACATTTAACCTCAAAGAAACTCGTGGATGATGGATTCATGTTCAAGTATAGCCTTGAGGAAATGTTTGAGGACGCAATTGAATGTTGCAAGGAAAAGGGTTATCTTTGA

Protein sequence

>MS.gene27777.t1

MDESYWSDVNILRNLKPFAWSYAVSKTLAEKAVLEFGEQHGLDIVTLIPTFVVGPFICPKLPGSVHSSLAFLFGDIDKNPLVLVSRTGMVHVDDVARAHIFLLEHPDPKGRYNCSPFIATIEEIVDLVSSKYPELQMPTSKELMGVKGPKFPHLTSKKLVDDGFMFKYSLEEMFEDAIECCKEKGYL