AlfalfaGEDB | MS.gene27844

M. sativa cultivar XinJiangDaYe / MS.gene27844

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27844.t1	GAU18803.1	88.6	236	21	5	16	248	82	314	2.20E-98	368.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27844.t1	Q8LEE9	56.2	169	66	5	13	178	26	189	1.0e-38	161.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27844.t1	A0A2Z6M9N0	88.6	236	21	5	16	248	82	314	1.6e-98	368.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056470	MS.gene27844	0.904732	9.02E-80	-1.69E-46
MS.gene058848	MS.gene27844	0.84826	6.87E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 63 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTCAATGGTGGGTCCTTT+TGG	0.235120	4.1:+13187462	None:intergenic
GCCTCTTGATCTTGTTCTTC+CGG	0.272392	4.1:-13187007	MS.gene27844:CDS
TAGCAAACTTGCTATTTATC+AGG	0.275231	4.1:-13187046	MS.gene27844:CDS
GAGGAACCCTGCTTTGAGTT+TGG	0.336926	4.1:+13187287	None:intergenic
GCAGTTAATGCTACCGTTAC+TGG	0.358591	4.1:-13187084	MS.gene27844:CDS
TTGGAGAATGCAGAATCTTC+TGG	0.359282	4.1:+13187306	None:intergenic
GTTGGTGGAGTTGTTCTATA+AGG	0.359667	4.1:-13186793	None:intergenic
CCTGCTGATGTTTCTGCTGC+AGG	0.360987	4.1:-13186859	MS.gene27844:CDS
TTTGGCTCCTGCACCGGCTA+AGG	0.362528	4.1:-13186971	MS.gene27844:CDS
CAGTGGCAATCAAGTTAGTA+TGG	0.363282	4.1:-13187115	MS.gene27844:CDS
TCCGGCTAAGGCTCCAGCTT+TGG	0.363612	4.1:-13186989	MS.gene27844:CDS
TCACCGGCTTGTGTTTGAAC+AGG	0.366430	4.1:+13187171	None:intergenic
TGCTATTTATCAGGTTGATA+AGG	0.394299	4.1:-13187037	MS.gene27844:CDS
AATTAGAACGGAGAACCTTT+TGG	0.395304	4.1:+13187419	None:intergenic
TTTGGAATGGGTTTGGTTGG+TGG	0.406116	4.1:-13186808	MS.gene27844:CDS
GGTGCACCAGTTGGTGTTGT+TGG	0.418778	4.1:+13187504	None:intergenic
TGATCTTGTTCTTCCGGCTA+AGG	0.420269	4.1:-13187001	MS.gene27844:CDS
GCCGGTGCAGGAGCCAAAGC+TGG	0.431263	4.1:+13186976	None:intergenic
TGTTTGAACAGGGTTGGTTA+AGG	0.439720	4.1:+13187182	None:intergenic
CTGCTGCAGGATCTGTGCAT+TGG	0.442652	4.1:-13186846	MS.gene27844:CDS
AAGGGTTTGTTGCCTAAGGC+TGG	0.443081	4.1:-13186952	MS.gene27844:CDS
GTATCATCACCATCAGGTTC+AGG	0.443291	4.1:-13187348	MS.gene27844:CDS
CTTGTTCCAACAACACCAAC+TGG	0.444147	4.1:-13187510	MS.gene27844:CDS
AAGAATGTTGATTATGTCAA+TGG	0.455371	4.1:+13187449	None:intergenic
TTGGCTCCTGCACCGGCTAA+GGG	0.463641	4.1:-13186970	MS.gene27844:CDS
AACAAACCCTTAGCCGGTGC+AGG	0.463951	4.1:+13186964	None:intergenic
GCATTCTCCAAACTCAAAGC+AGG	0.466381	4.1:-13187294	MS.gene27844:CDS
GGCTAAGGGTTTGTTGCCTA+AGG	0.468336	4.1:-13186956	MS.gene27844:CDS
AATTCTTCGGTTGTAGATGA+TGG	0.470488	4.1:-13186922	MS.gene27844:CDS
GTAGATGATGGTAGCGGTGC+TGG	0.479324	4.1:-13186910	MS.gene27844:CDS
TCCAGCTTTGGCTCCTGCAC+CGG	0.487588	4.1:-13186977	MS.gene27844:CDS
GTCTATCAGTGAGGGAGTTG+AGG	0.498053	4.1:+13187268	None:intergenic
GGCTTGTGTTTGAACAGGGT+TGG	0.506531	4.1:+13187176	None:intergenic
CACCGGCTTGTGTTTGAACA+GGG	0.508119	4.1:+13187172	None:intergenic
ATACTAGCTGCGAGTTGAGT+TGG	0.509205	4.1:+13187367	None:intergenic
GCCAAAGCTGGAGCCTTAGC+CGG	0.517768	4.1:+13186988	None:intergenic
AACGGTAGCATTAACTGCTC+CGG	0.527378	4.1:+13187089	None:intergenic
CAGCTAGTATCATCACCATC+AGG	0.530331	4.1:-13187354	MS.gene27844:CDS
CCCACCAAAGCAATCCCAAA+AGG	0.532318	4.1:-13187477	MS.gene27844:CDS
AAATAGAAACAAACGAAGCA+AGG	0.540997	4.1:+13187217	None:intergenic
AGCGGTGCTGGCAGTGATGA+TGG	0.560075	4.1:-13186898	MS.gene27844:CDS
TTAGGCAACAAACCCTTAGC+CGG	0.560846	4.1:+13186958	None:intergenic
ATAAATAGCAAGTTTGCTAT+CGG	0.562446	4.1:+13187050	None:intergenic
GTTGGTGTTGTTGGAACAAG+CGG	0.574823	4.1:+13187513	None:intergenic
AACCCTGTTCAAACACAAGC+CGG	0.576254	4.1:-13187174	MS.gene27844:CDS
CACAAGCCGGTGATGATGCT+AGG	0.584174	4.1:-13187161	MS.gene27844:CDS
AATCAAGTTAGTATGGCTAC+CGG	0.593663	4.1:-13187108	MS.gene27844:CDS
TCGGTTGTAGATGATGGTAG+CGG	0.599850	4.1:-13186916	MS.gene27844:CDS
CCTGCAGCAGAAACATCAGC+AGG	0.607919	4.1:+13186859	None:intergenic
TCATCACCATCAGGTTCAGG+AGG	0.610251	4.1:-13187345	MS.gene27844:CDS
CATTCTCCAAACTCAAAGCA+GGG	0.610410	4.1:-13187293	MS.gene27844:CDS
TTTGAGAAGACGAATTAGAA+CGG	0.612652	4.1:+13187407	None:intergenic
GCCGGAAGAACAAGATCAAG+AGG	0.613702	4.1:+13187006	None:intergenic
AATGTTGATTATGTCAATGG+TGG	0.616748	4.1:+13187452	None:intergenic
AATAGAAACAAACGAAGCAA+GGG	0.620497	4.1:+13187218	None:intergenic
TTGAAGCCTAGCATCATCAC+CGG	0.621788	4.1:+13187155	None:intergenic
CAGTGATGATGGTGATGGAA+AGG	0.627687	4.1:-13186887	MS.gene27844:CDS
GGTATAAACAGTACCAGTAA+CGG	0.640082	4.1:+13187071	None:intergenic
GCTGGCAGTGATGATGGTGA+TGG	0.645590	4.1:-13186892	MS.gene27844:CDS
GTTAACCCTCCTGAACCTGA+TGG	0.664853	4.1:+13187339	None:intergenic
CTGAACGTCACTACTTACAG+TGG	0.677815	4.1:-13187132	MS.gene27844:CDS
ATGTTGATTATGTCAATGGT+GGG	0.681774	4.1:+13187453	None:intergenic
CATCACCATCAGGTTCAGGA+GGG	0.700637	4.1:-13187344	MS.gene27844:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATGTTGATTATGTCAA+TGG	+	chr4.1:13186869-13186888	None:intergenic	25.0%
!!!	AATGTTGTTGTTTTTGGAAT+GGG	-	chr4.1:13187495-13187514	MS.gene27844:CDS	25.0%
!!!	ATAAATAGCAAGTTTGCTAT+CGG	+	chr4.1:13187268-13187287	None:intergenic	25.0%
	AAATAGAAACAAACGAAGCA+AGG	+	chr4.1:13187101-13187120	None:intergenic	30.0%
	AATAGAAACAAACGAAGCAA+GGG	+	chr4.1:13187100-13187119	None:intergenic	30.0%
	ACATTCTTCAAAAAGCCAAA+AGG	-	chr4.1:13186881-13186900	MS.gene27844:CDS	30.0%
	TTTGAGAAGACGAATTAGAA+CGG	+	chr4.1:13186911-13186930	None:intergenic	30.0%
!	AACTTTTTGTCTATCAGTGA+GGG	+	chr4.1:13187058-13187077	None:intergenic	30.0%
!	AATGTTGATTATGTCAATGG+TGG	+	chr4.1:13186866-13186885	None:intergenic	30.0%
!	ATGTTGATTATGTCAATGGT+GGG	+	chr4.1:13186865-13186884	None:intergenic	30.0%
!!	TAGCAAACTTGCTATTTATC+AGG	-	chr4.1:13187269-13187288	MS.gene27844:CDS	30.0%
!!	TGCTATTTATCAGGTTGATA+AGG	-	chr4.1:13187278-13187297	MS.gene27844:CDS	30.0%
!!!	GAATGTTGTTGTTTTTGGAA+TGG	-	chr4.1:13187494-13187513	MS.gene27844:CDS	30.0%
	AATCAAGTTAGTATGGCTAC+CGG	-	chr4.1:13187207-13187226	MS.gene27844:CDS	35.0%
	AATTCTTCGGTTGTAGATGA+TGG	-	chr4.1:13187393-13187412	MS.gene27844:CDS	35.0%
	AGAATCTTCTGGTGCAAAAA+TGG	+	chr4.1:13187001-13187020	None:intergenic	35.0%
	GGCTGGAAAAACAAATTCTT+CGG	-	chr4.1:13187380-13187399	MS.gene27844:CDS	35.0%
	GGTATAAACAGTACCAGTAA+CGG	+	chr4.1:13187247-13187266	None:intergenic	35.0%
!	AATTAGAACGGAGAACCTTT+TGG	+	chr4.1:13186899-13186918	None:intergenic	35.0%
!	CAACTTTTTGTCTATCAGTG+AGG	+	chr4.1:13187059-13187078	None:intergenic	35.0%
!!	GAATTTGTTTTTCCAGCCTT+AGG	+	chr4.1:13187378-13187397	None:intergenic	35.0%
!!!	TGGCTGAATGTTGTTGTTTT+TGG	-	chr4.1:13187489-13187508	MS.gene27844:CDS	35.0%
!!!	TGTTGTTTTTGGAATGGGTT+TGG	-	chr4.1:13187500-13187519	MS.gene27844:CDS	35.0%
	CAGTGGCAATCAAGTTAGTA+TGG	-	chr4.1:13187200-13187219	MS.gene27844:CDS	40.0%
	CATTCTCCAAACTCAAAGCA+GGG	-	chr4.1:13187022-13187041	MS.gene27844:CDS	40.0%
	TTGGAGAATGCAGAATCTTC+TGG	+	chr4.1:13187012-13187031	None:intergenic	40.0%
!!	TGTTTGAACAGGGTTGGTTA+AGG	+	chr4.1:13187136-13187155	None:intergenic	40.0%
!!!	GTTTTTGGAATGGGTTTGGT+TGG	-	chr4.1:13187504-13187523	MS.gene27844:CDS	40.0%
	AACCCTGTTCAAACACAAGC+CGG	-	chr4.1:13187141-13187160	MS.gene27844:CDS	45.0%
	AACGGTAGCATTAACTGCTC+CGG	+	chr4.1:13187229-13187248	None:intergenic	45.0%
	CAGCTAGTATCATCACCATC+AGG	-	chr4.1:13186961-13186980	MS.gene27844:CDS	45.0%
	CTGAACGTCACTACTTACAG+TGG	-	chr4.1:13187183-13187202	MS.gene27844:CDS	45.0%
	CTTGTTCCAACAACACCAAC+TGG	-	chr4.1:13186805-13186824	MS.gene27844:CDS	45.0%
	GCAGTTAATGCTACCGTTAC+TGG	-	chr4.1:13187231-13187250	MS.gene27844:CDS	45.0%
	GCATTCTCCAAACTCAAAGC+AGG	-	chr4.1:13187021-13187040	MS.gene27844:CDS	45.0%
	GCCTCTTGATCTTGTTCTTC+CGG	-	chr4.1:13187308-13187327	MS.gene27844:CDS	45.0%
	GTATCATCACCATCAGGTTC+AGG	-	chr4.1:13186967-13186986	MS.gene27844:CDS	45.0%
	TGATCTTGTTCTTCCGGCTA+AGG	-	chr4.1:13187314-13187333	MS.gene27844:CDS	45.0%
	TTAGGCAACAAACCCTTAGC+CGG	+	chr4.1:13187360-13187379	None:intergenic	45.0%
	TTGAAGCCTAGCATCATCAC+CGG	+	chr4.1:13187163-13187182	None:intergenic	45.0%
!	ATACTAGCTGCGAGTTGAGT+TGG	+	chr4.1:13186951-13186970	None:intergenic	45.0%
!	TCGGTTGTAGATGATGGTAG+CGG	-	chr4.1:13187399-13187418	MS.gene27844:CDS	45.0%
!	TTTGGAATGGGTTTGGTTGG+TGG	-	chr4.1:13187507-13187526	MS.gene27844:CDS	45.0%
!!	CAGTGATGATGGTGATGGAA+AGG	-	chr4.1:13187428-13187447	MS.gene27844:CDS	45.0%
!!	GTTGGTGTTGTTGGAACAAG+CGG	+	chr4.1:13186805-13186824	None:intergenic	45.0%
!!!	ATGTCAATGGTGGGTCCTTT+TGG	+	chr4.1:13186856-13186875	None:intergenic	45.0%
!!!	CCTTTTGGGATTGCTTTGGT+GGG	+	chr4.1:13186841-13186860	None:intergenic	45.0%
!!!	CTTTTGGGATTGCTTTGGTG+GGG	+	chr4.1:13186840-13186859	None:intergenic	45.0%
!!!	TCCTTTTGGGATTGCTTTGG+TGG	+	chr4.1:13186842-13186861	None:intergenic	45.0%
!!!	TGTCAATGGTGGGTCCTTTT+GGG	+	chr4.1:13186855-13186874	None:intergenic	45.0%
!!!	TTTTGGGATTGCTTTGGTGG+GGG	+	chr4.1:13186839-13186858	None:intergenic	45.0%
	CATCACCATCAGGTTCAGGA+GGG	-	chr4.1:13186971-13186990	MS.gene27844:CDS	50.0%
	CCCACCAAAGCAATCCCAAA+AGG	-	chr4.1:13186838-13186857	MS.gene27844:CDS	50.0%
	GCCGGAAGAACAAGATCAAG+AGG	+	chr4.1:13187312-13187331	None:intergenic	50.0%
	GGCTTGTGTTTGAACAGGGT+TGG	+	chr4.1:13187142-13187161	None:intergenic	50.0%
	GTCTATCAGTGAGGGAGTTG+AGG	+	chr4.1:13187050-13187069	None:intergenic	50.0%
	GTTAACCCTCCTGAACCTGA+TGG	+	chr4.1:13186979-13186998	None:intergenic	50.0%
	TCATCACCATCAGGTTCAGG+AGG	-	chr4.1:13186970-13186989	MS.gene27844:CDS	50.0%
!	GAGGAACCCTGCTTTGAGTT+TGG	+	chr4.1:13187031-13187050	None:intergenic	50.0%
!!	AAGGGTTTGTTGCCTAAGGC+TGG	-	chr4.1:13187363-13187382	MS.gene27844:CDS	50.0%
!!	CACCGGCTTGTGTTTGAACA+GGG	+	chr4.1:13187146-13187165	None:intergenic	50.0%
!!	GGCTAAGGGTTTGTTGCCTA+AGG	-	chr4.1:13187359-13187378	MS.gene27844:CDS	50.0%
!!	TCACCGGCTTGTGTTTGAAC+AGG	+	chr4.1:13187147-13187166	None:intergenic	50.0%
!!!	GGGTCCTTTTGGGATTGCTT+TGG	+	chr4.1:13186845-13186864	None:intergenic	50.0%
	AACAAACCCTTAGCCGGTGC+AGG	+	chr4.1:13187354-13187373	None:intergenic	55.0%
	CCTGCAGCAGAAACATCAGC+AGG	+	chr4.1:13187459-13187478	None:intergenic	55.0%
	CTGCTGCAGGATCTGTGCAT+TGG	-	chr4.1:13187469-13187488	MS.gene27844:CDS	55.0%
!	CACAAGCCGGTGATGATGCT+AGG	-	chr4.1:13187154-13187173	MS.gene27844:CDS	55.0%
!	CCTGCTGATGTTTCTGCTGC+AGG	-	chr4.1:13187456-13187475	MS.gene27844:CDS	55.0%
!!	GCTGGCAGTGATGATGGTGA+TGG	-	chr4.1:13187423-13187442	MS.gene27844:CDS	55.0%
!!	GGTGCACCAGTTGGTGTTGT+TGG	+	chr4.1:13186814-13186833	None:intergenic	55.0%
!!	GTAGATGATGGTAGCGGTGC+TGG	-	chr4.1:13187405-13187424	MS.gene27844:CDS	55.0%
	GCCAAAGCTGGAGCCTTAGC+CGG	+	chr4.1:13187330-13187349	None:intergenic	60.0%
	GGGATTGCTTTGGTGGGGGA+TGG	+	chr4.1:13186835-13186854	None:intergenic	60.0%
	TCCGGCTAAGGCTCCAGCTT+TGG	-	chr4.1:13187326-13187345	MS.gene27844:CDS	60.0%
!	TCCAGCTTTGGCTCCTGCAC+CGG	-	chr4.1:13187338-13187357	MS.gene27844:CDS	60.0%
!!	AGCGGTGCTGGCAGTGATGA+TGG	-	chr4.1:13187417-13187436	MS.gene27844:CDS	60.0%
!!	TTGGCTCCTGCACCGGCTAA+GGG	-	chr4.1:13187345-13187364	MS.gene27844:CDS	60.0%
!!	TTTGGCTCCTGCACCGGCTA+AGG	-	chr4.1:13187344-13187363	MS.gene27844:CDS	60.0%
	GTGGGGGATGGTGCACCAGT+TGG	+	chr4.1:13186823-13186842	None:intergenic	65.0%
	GCCGGTGCAGGAGCCAAAGC+TGG	+	chr4.1:13187342-13187361	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	13186795	13187542	13186795	ID=MS.gene27844
chr4.1	mRNA	13186795	13187542	13186795	ID=MS.gene27844.t1;Parent=MS.gene27844
chr4.1	exon	13186795	13187542	13186795	ID=MS.gene27844.t1.exon1;Parent=MS.gene27844.t1
chr4.1	CDS	13186795	13187542	13186795	ID=cds.MS.gene27844.t1;Parent=MS.gene27844.t1

Gene Sequence

>MS.gene27844

CACAGTTCCGCTTGTTCCAACAACACCAACTGGTGCACCATCCCCCACCAAAGCAATCCCAAAAGGACCCACCATTGACATAATCAACATTCTTCAAAAAGCCAAAAGGTTCTCCGTTCTAATTCGTCTTCTCAAAACAACACAATTAATAAACCAACTCAACTCGCAGCTAGTATCATCACCATCAGGTTCAGGAGGGTTAACCATTTTTGCACCAGAAGATTCTGCATTCTCCAAACTCAAAGCAGGGTTCCTCAACTCCCTCACTGATAGACAAAAAGTTGAACTCTTACAGTTTCACTCCCTTGCTTCGTTTGTTTCTATTTCAAACTTTGATACCTTAACCAACCCTGTTCAAACACAAGCCGGTGATGATGCTAGGCTTCAACTGAACGTCACTACTTACAGTGGCAATCAAGTTAGTATGGCTACCGGAGCAGTTAATGCTACCGTTACTGGTACTGTTTATACCGATAGCAAACTTGCTATTTATCAGGTTGATAAGGTACTTATGCCTCTTGATCTTGTTCTTCCGGCTAAGGCTCCAGCTTTGGCTCCTGCACCGGCTAAGGGTTTGTTGCCTAAGGCTGGAAAAACAAATTCTTCGGTTGTAGATGATGGTAGCGGTGCTGGCAGTGATGATGGTGATGGAAAGGCTTTACCTGCTGATGTTTCTGCTGCAGGATCTGTGCATTGGCTGAATGTTGTTGTTTTTGGAATGGGTTTGGTTGGTGGAGTTGTTCTATAA

Protein sequence

>MS.gene27844.t1

HSSACSNNTNWCTIPHQSNPKRTHHUHNQHSSKSQKVLRSNSSSQNNTINKPTQLAASIITIRFRRVNHFCTRRFCILQTQSRVPQLPHUUTKSUTLTVSLPCFVCFYFKLUYLNQPCSNTSRUUCUASTERHYLQWQSSUYGYRSSUCYRYWYCLYRUQTCYLSGUUGTYASUSCSSGUGSSFGSCTGUGFVAUGWKNKFFGCRUWURCWQUUWUWKGFTCUCFCCRICALAECCCFWNGFGWWSCSI