AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27856

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27856.t1 MTR_8g087750 88.791 678 15 2 1 617 1 678 0.0 1193
MS.gene27856.t1 MTR_8g087770 73.621 671 107 7 11 616 6 671 0.0 956
MS.gene27856.t1 MTR_8g087763 57.778 225 56 1 354 539 4 228 8.36e-81 259
MS.gene27856.t1 MTR_4g093750 39.716 282 148 8 69 333 35 311 2.34e-42 163
MS.gene27856.t1 MTR_4g093750 39.716 282 148 8 69 333 35 311 2.57e-42 163
MS.gene27856.t1 MTR_2g009690 36.027 297 172 7 65 344 40 335 1.77e-39 155
MS.gene27856.t1 MTR_5g009150 44.385 187 100 3 71 254 35 220 4.15e-34 137
MS.gene27856.t1 MTR_7g451010 40.094 212 117 5 71 278 40 245 8.82e-32 130
MS.gene27856.t1 MTR_8g105580 38.333 240 126 7 71 295 35 267 1.98e-31 129
MS.gene27856.t1 MTR_8g027675 38.389 211 123 4 71 276 34 242 2.04e-31 129
MS.gene27856.t1 MTR_8g105580 38.333 240 126 7 71 295 35 267 2.31e-31 129
MS.gene27856.t1 MTR_1g032550 40.107 187 108 3 71 254 35 220 3.12e-31 129
MS.gene27856.t1 MTR_8g099550 42.246 187 104 3 71 254 34 219 4.40e-31 129
MS.gene27856.t1 MTR_4g098920 41.063 207 106 6 71 270 34 231 1.57e-30 127
MS.gene27856.t1 MTR_8g009510 40.196 204 112 5 71 270 40 237 1.87e-29 124
MS.gene27856.t1 MTR_4g085860 72.368 76 20 1 208 282 2 77 3.03e-29 111
MS.gene27856.t1 MTR_6g045107 71.053 76 21 1 208 282 2 77 2.77e-27 105
MS.gene27856.t1 MTR_8g009510 36.567 134 79 2 138 270 2 130 5.62e-15 78.6
MS.gene27856.t1 MTR_8g463270 28.631 241 147 9 56 281 27 257 6.40e-15 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27856.t1 AT1G60500 54.134 641 229 6 35 612 29 667 0.0 684
MS.gene27856.t1 AT1G60530 70.934 289 78 1 22 310 19 301 3.00e-141 412
MS.gene27856.t1 AT4G33650 34.796 319 184 10 39 340 31 342 1.77e-38 152
MS.gene27856.t1 AT4G33650 34.796 319 184 10 39 340 31 342 1.81e-38 152
MS.gene27856.t1 AT2G14120 33.127 323 191 9 36 340 12 327 3.48e-36 145
MS.gene27856.t1 AT2G14120 33.127 323 191 9 36 340 12 327 3.64e-36 145
MS.gene27856.t1 AT2G14120 33.127 323 191 9 36 340 12 327 3.68e-36 145
MS.gene27856.t1 AT2G14120 33.127 323 191 9 36 340 12 327 4.33e-36 145
MS.gene27856.t1 AT1G14830 42.857 182 100 3 71 249 35 215 1.28e-32 133
MS.gene27856.t1 AT5G42080 39.810 211 121 4 71 276 34 243 1.48e-31 127
MS.gene27856.t1 AT2G44590 32.075 318 179 9 71 356 35 347 1.90e-31 130
MS.gene27856.t1 AT3G60190 39.810 211 121 4 71 276 40 249 3.08e-31 129
MS.gene27856.t1 AT5G42080 39.810 211 121 4 71 276 34 243 3.25e-31 129
MS.gene27856.t1 AT5G42080 39.810 211 121 4 71 276 34 243 3.35e-31 129
MS.gene27856.t1 AT5G42080 39.810 211 121 4 71 276 34 243 3.35e-31 129
MS.gene27856.t1 AT3G61760 41.667 204 109 5 71 270 34 231 5.10e-31 127
MS.gene27856.t1 AT3G61760 41.667 204 109 5 71 270 34 231 7.15e-31 128
MS.gene27856.t1 AT3G61760 41.667 204 109 5 71 270 34 231 1.07e-30 127
MS.gene27856.t1 AT2G44590 29.245 318 171 10 71 356 35 330 6.25e-22 100
MS.gene27856.t1 AT2G44590 29.245 318 171 10 71 356 35 330 6.62e-22 100
MS.gene27856.t1 AT1G53140 23.193 332 231 7 1 323 1 317 1.62e-14 77.4

Find 104 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGTCTCCGTCTATTTC+TGG 0.150723 4.1:+13047791 MS.gene27856:CDS
AGTCTCACTCACTTCTTTCT+AGG 0.252212 4.1:+13046740 MS.gene27856:CDS
ATTCTGAAGGCGCATAATTA+AGG 0.255203 4.1:-13046179 None:intergenic
TTACTACTTGTGAGTCTATC+AGG 0.258224 4.1:+13046524 MS.gene27856:CDS
TGGAAGCTGAATTCCTTCTT+TGG 0.309988 4.1:-13046053 None:intergenic
TTCAAATTATTATCAGCTTC+AGG 0.310159 4.1:+13047322 MS.gene27856:CDS
TTCTCAAGGGCGTCGAGAAC+TGG 0.318424 4.1:-13046010 None:intergenic
AGTATTTGCTCGAGTATAAT+AGG 0.323700 4.1:+13047459 MS.gene27856:CDS
CTTCCAACAATTGTTGTTGT+TGG 0.331397 4.1:+13046070 MS.gene27856:CDS
GGAGACTGTTGCAAGAATTA+TGG 0.333112 4.1:+13047862 MS.gene27856:CDS
GTTGTTGGAGATCAATCTTC+AGG 0.340523 4.1:+13046085 MS.gene27856:CDS
TCACAAATGGTTGATAAAAC+TGG 0.343252 4.1:+13046550 MS.gene27856:CDS
TTGAATTCTAACAAGAGTTC+TGG 0.352154 4.1:-13046217 None:intergenic
TTCAAGATAATACTTTCTTC+AGG 0.356152 4.1:-13046478 None:intergenic
CTTACCATGGTTGATCTTCC+AGG 0.356170 4.1:+13046376 MS.gene27856:CDS
TCAGATAAAAGATATTATTA+TGG 0.384953 4.1:+13046444 MS.gene27856:CDS
AGGCTAGGATGATTGCTTAC+TGG 0.386618 4.1:+13047627 MS.gene27856:CDS
AAGTTCAGTCCAACATGTTA+TGG 0.389392 4.1:+13047391 MS.gene27856:CDS
ACCTTCTCCAACAATCCTTC+AGG 0.403379 4.1:-13046613 None:intergenic
CAAAGTTCTTAGAGAAAGTA+AGG 0.404659 4.1:+13047841 MS.gene27856:CDS
CATAGGTCTCATCTCCAATT+CGG 0.409654 4.1:-13046684 None:intergenic
TCTCCAACAACAACAATTGT+TGG 0.419207 4.1:-13046073 None:intergenic
GTCTGTGTGCGAAACCGAAT+TGG 0.426739 4.1:+13046670 MS.gene27856:CDS
TCGACGCCCTTGAGAATCTA+AGG 0.430085 4.1:+13046017 MS.gene27856:CDS
ATCTGATCATAGATATTGTC+AGG 0.431759 4.1:-13046427 None:intergenic
CAGGTTTCCACTCGCAGAGC+AGG 0.433533 4.1:+13047341 MS.gene27856:CDS
CCAACACATGTGAAGCTTGA+AGG 0.436407 4.1:+13047536 MS.gene27856:CDS
CAATGCATGTGCTTGTCCTC+AGG 0.447247 4.1:-13047018 None:intergenic
AGGATTGACAAATCCATTGT+TGG 0.451509 4.1:+13046760 MS.gene27856:CDS
TAAATCTCCTGAAGGATTGT+TGG 0.453191 4.1:+13046606 MS.gene27856:CDS
GACCTATGAAGATGCTAGAT+TGG 0.455034 4.1:+13046699 MS.gene27856:CDS
ATCACCCGTGTACCTGTTCA+TGG 0.456607 4.1:+13046400 MS.gene27856:CDS
GAACTCTTGTTAGAATTCAA+TGG 0.458145 4.1:+13046220 MS.gene27856:CDS
TATGGAAGAGATTAAGGTGT+TGG 0.462977 4.1:+13047125 MS.gene27856:CDS
TTGGCATAGACCAATCCATT+TGG 0.463236 4.1:-13047154 None:intergenic
TGATACCAGCTAAAGATTCA+AGG 0.464998 4.1:-13046120 None:intergenic
TTCTGAAGGCGCATAATTAA+GGG 0.465234 4.1:-13046178 None:intergenic
AGTGTCCTTGAATCTTTAGC+TGG 0.467085 4.1:+13046115 MS.gene27856:CDS
AAGAGTTCTGGCTTTGAAAG+TGG 0.480074 4.1:-13046205 None:intergenic
CCTTCAAGCTTCACATGTGT+TGG 0.480389 4.1:-13047536 None:intergenic
CGTCTCAACATTGCCAAAGA+AGG 0.485759 4.1:+13046040 MS.gene27856:CDS
CTCTAAGAACTTTGACACTT+CGG 0.488083 4.1:-13047832 None:intergenic
CTCCGTCTATTTCTGGAAAG+AGG 0.488105 4.1:+13047798 MS.gene27856:CDS
ATAGCTACTGAAGAGATTGC+TGG 0.488901 4.1:+13046289 MS.gene27856:CDS
TCATTGTAGGATGAAACAAT+TGG 0.490493 4.1:-13045977 None:intergenic
TTGTCAGGTTGACCATGAAC+AGG 0.495557 4.1:-13046412 None:intergenic
AGGTGTTGGAAGAGGCCAAA+TGG 0.496352 4.1:+13047139 MS.gene27856:CDS
ACAGGTACACGGGTGATACC+TGG 0.496559 4.1:-13046394 None:intergenic
TTACCCTCATGTGCTAAGTC+AGG 0.501551 4.1:+13047592 MS.gene27856:CDS
GCAACAGAAGATAAATTAGC+AGG 0.505796 4.1:-13046907 None:intergenic
GCAAGAATTATGGACAGGAT+TGG 0.508761 4.1:+13047872 MS.gene27856:CDS
AGATCAACCATGGTAAGATC+AGG 0.510480 4.1:-13046370 None:intergenic
AATCAAAGTCAAAGGAGTGT+TGG 0.514073 4.1:-13046329 None:intergenic
TTCCAATCTAGCATCTTCAT+AGG 0.522397 4.1:-13046701 None:intergenic
GTCTATCAGGATGTCACAAA+TGG 0.523985 4.1:+13046537 MS.gene27856:CDS
GATGACGTTAACATCGGTCT+AGG 0.524437 4.1:+13046643 MS.gene27856:CDS
CTCTCAATCCCACCACCACT+TGG 0.524638 4.1:-13047761 None:intergenic
TTGGAAGAGGCCAAATGGAT+TGG 0.525795 4.1:+13047144 MS.gene27856:CDS
AGAGATTAAGGTGTTGGAAG+AGG 0.526369 4.1:+13047131 MS.gene27856:CDS
TTCTTGACAATCAAAGTCAA+AGG 0.538928 4.1:-13046337 None:intergenic
ATCAGCTTGCCACGTGGACA+AGG 0.540401 4.1:+13046139 MS.gene27856:CDS
CTGTTGCAAGAATTATGGAC+AGG 0.542640 4.1:+13047867 MS.gene27856:CDS
GAGGTTGAGCTTGCTCAACA+TGG 0.552600 4.1:-13045940 None:intergenic
AGGAGTGTTGGAAATCCCTC+TGG 0.553456 4.1:-13046317 None:intergenic
AATGCATGTGCTTGTCCTCA+GGG 0.555069 4.1:-13047017 None:intergenic
CAAGTGGTGGTGGGATTGAG+AGG 0.561054 4.1:+13047762 MS.gene27856:CDS
TGGTTGATAAAACTGGTTTG+AGG 0.562514 4.1:+13046557 MS.gene27856:CDS
ATTGGTGCAACAACAGAAAG+AGG 0.569115 4.1:-13045959 None:intergenic
TCCTGAAGGATTGTTGGAGA+AGG 0.571650 4.1:+13046612 MS.gene27856:CDS
AGCTAAAGATTCAAGGACAC+TGG 0.572468 4.1:-13046113 None:intergenic
TAAAATCAACAGTGGCGGAA+AGG 0.574096 4.1:-13046504 None:intergenic
TCCCTCTTTCCAGAAATAGA+CGG 0.574959 4.1:-13047800 None:intergenic
TCCGTCTATTTCTGGAAAGA+GGG 0.578371 4.1:+13047799 MS.gene27856:CDS
AAAGTGATGATTCATCTCGT+TGG 0.587870 4.1:-13045910 None:intergenic
ACTGGAAGATTGTTCTGAGG+AGG 0.593317 4.1:+13047645 MS.gene27856:CDS
CAACAGCTTCCATAACATGT+TGG 0.595030 4.1:-13047400 None:intergenic
TGTTATGGAAGCTGTTGAGA+TGG 0.595595 4.1:+13047406 MS.gene27856:CDS
GATTAACAATGATTTGCAGA+AGG 0.597025 4.1:+13047715 MS.gene27856:CDS
AAAGCCTGACTTAGCACATG+AGG 0.603423 4.1:-13047596 None:intergenic
CTTACTGGAAGATTGTTCTG+AGG 0.604360 4.1:+13047642 MS.gene27856:CDS
TTGAAAGTGGATGATTCTGA+AGG 0.605131 4.1:-13046192 None:intergenic
GAGATTGCTGGTTCTGCCAG+AGG 0.608773 4.1:+13046301 MS.gene27856:CDS
GTTGACCATGAACAGGTACA+CGG 0.609991 4.1:-13046405 None:intergenic
AGATTGCTGGTTCTGCCAGA+GGG 0.611506 4.1:+13046302 MS.gene27856:CDS
TTTCTCGCCTGCTCTGCGAG+TGG 0.614106 4.1:-13047348 None:intergenic
TGAGACGCCTTAGATTCTCA+AGG 0.620323 4.1:-13046024 None:intergenic
AAAGCAGATAAATCTCCTGA+AGG 0.623780 4.1:+13046598 MS.gene27856:CDS
GAGACGCCTTAGATTCTCAA+GGG 0.625868 4.1:-13046023 None:intergenic
TAAAACAGGAATACCAACAA+TGG 0.629558 4.1:-13046773 None:intergenic
TTATACTCGAGCAAATACTC+AGG 0.634135 4.1:-13047455 None:intergenic
TTGACCATGAACAGGTACAC+GGG 0.647300 4.1:-13046404 None:intergenic
AAATTGATGTTGTTAATCTG+AGG 0.650762 4.1:+13047567 MS.gene27856:CDS
AAGCCTGACTTAGCACATGA+GGG 0.658164 4.1:-13047595 None:intergenic
AGTAGTAAAATCAACAGTGG+CGG 0.662087 4.1:-13046509 None:intergenic
GTGCAGATTCCTTGTCCACG+TGG 0.668352 4.1:-13046148 None:intergenic
CATGTGAAGCTTGAAGGTGT+TGG 0.674753 4.1:+13047542 MS.gene27856:CDS
TGGTGTTCCTGATCTTACCA+TGG 0.685138 4.1:+13046363 MS.gene27856:CDS
ACTGCTGATGACGTTAACAT+CGG 0.693473 4.1:+13046637 MS.gene27856:CDS
GCTGGTATCAGCTTGCCACG+TGG 0.693476 4.1:+13046133 MS.gene27856:CDS
GTGGACAAGGAATCTGCACG+AGG 0.706834 4.1:+13046152 MS.gene27856:CDS
ACAAGTAGTAAAATCAACAG+TGG 0.707970 4.1:-13046512 None:intergenic
GATACCTGGAAGATCAACCA+TGG 0.712735 4.1:-13046380 None:intergenic
TGGACAAGGAATCTGCACGA+GGG 0.740882 4.1:+13046153 MS.gene27856:CDS
TGATTTCGAAGAGTACCCTG+AGG 0.804578 4.1:+13047002 MS.gene27856:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAGATAAAAGATATTATTA+TGG + chr4.1:13046444-13046463 MS.gene27856:CDS 15.0%
! AAATTGATGTTGTTAATCTG+AGG + chr4.1:13047567-13047586 MS.gene27856:CDS 25.0%
! ACTTTGATTGTCAAGAAAAA+TGG + chr4.1:13046343-13046362 MS.gene27856:CDS 25.0%
! TTCAAATTATTATCAGCTTC+AGG + chr4.1:13047322-13047341 MS.gene27856:CDS 25.0%
! TTCAAGATAATACTTTCTTC+AGG - chr4.1:13046481-13046500 None:intergenic 25.0%
!! TGCGAAAAAGAATTTTCTTA+TGG + chr4.1:13047107-13047126 MS.gene27856:CDS 25.0%
!! TTTTCTTATGGAAGAGATTA+AGG + chr4.1:13047119-13047138 MS.gene27856:CDS 25.0%
!!! CTAACTTTGTTGATAATGTT+TGG + chr4.1:13047255-13047274 MS.gene27856:intron 25.0%
AATACTGCAGAGAAAAGTTA+AGG + chr4.1:13047213-13047232 MS.gene27856:intron 30.0%
ACAAGTAGTAAAATCAACAG+TGG - chr4.1:13046515-13046534 None:intergenic 30.0%
AGTATTTGCTCGAGTATAAT+AGG + chr4.1:13047459-13047478 MS.gene27856:CDS 30.0%
ATACTGCAGAGAAAAGTTAA+GGG + chr4.1:13047214-13047233 MS.gene27856:intron 30.0%
ATCTGATCATAGATATTGTC+AGG - chr4.1:13046430-13046449 None:intergenic 30.0%
ATTGTCAAAAATGCAGTTCT+TGG - chr4.1:13047176-13047195 None:intergenic 30.0%
CAAAGTTCTTAGAGAAAGTA+AGG + chr4.1:13047841-13047860 MS.gene27856:CDS 30.0%
CTCAAAAAGATTCTTCTTAC+AGG + chr4.1:13046979-13046998 MS.gene27856:CDS 30.0%
GAACTCTTGTTAGAATTCAA+TGG + chr4.1:13046220-13046239 MS.gene27856:CDS 30.0%
TAAAACAGGAATACCAACAA+TGG - chr4.1:13046776-13046795 None:intergenic 30.0%
TCATTGTAGGATGAAACAAT+TGG - chr4.1:13045980-13045999 None:intergenic 30.0%
TTCTTGACAATCAAAGTCAA+AGG - chr4.1:13046340-13046359 None:intergenic 30.0%
TTGAATTCTAACAAGAGTTC+TGG - chr4.1:13046220-13046239 None:intergenic 30.0%
! ACAGCTTTTATGCATATTCT+TGG + chr4.1:13046940-13046959 MS.gene27856:CDS 30.0%
! GATTAACAATGATTTGCAGA+AGG + chr4.1:13047715-13047734 MS.gene27856:CDS 30.0%
! TTAATCTTCTTGATGATCTC+TGG - chr4.1:13046847-13046866 None:intergenic 30.0%
!! TCACAAATGGTTGATAAAAC+TGG + chr4.1:13046550-13046569 MS.gene27856:CDS 30.0%
!!! TGGACGTATTTTTTCATTGT+AGG - chr4.1:13045993-13046012 None:intergenic 30.0%
AAAGCAGATAAATCTCCTGA+AGG + chr4.1:13046598-13046617 MS.gene27856:CDS 35.0%
AAAGTGATGATTCATCTCGT+TGG - chr4.1:13045913-13045932 None:intergenic 35.0%
AAGTTAAGGGCAACATGTTA+TGG + chr4.1:13047227-13047246 MS.gene27856:intron 35.0%
AAGTTCAGTCCAACATGTTA+TGG + chr4.1:13047391-13047410 MS.gene27856:CDS 35.0%
AGGATTGACAAATCCATTGT+TGG + chr4.1:13046760-13046779 MS.gene27856:CDS 35.0%
AGTAGTAAAATCAACAGTGG+CGG - chr4.1:13046512-13046531 None:intergenic 35.0%
GCAACAGAAGATAAATTAGC+AGG - chr4.1:13046910-13046929 None:intergenic 35.0%
TAAATCTCCTGAAGGATTGT+TGG + chr4.1:13046606-13046625 MS.gene27856:CDS 35.0%
TATGGAAGAGATTAAGGTGT+TGG + chr4.1:13047125-13047144 MS.gene27856:CDS 35.0%
TCTCCAACAACAACAATTGT+TGG - chr4.1:13046076-13046095 None:intergenic 35.0%
TGATACCAGCTAAAGATTCA+AGG - chr4.1:13046123-13046142 None:intergenic 35.0%
TTACTACTTGTGAGTCTATC+AGG + chr4.1:13046524-13046543 MS.gene27856:CDS 35.0%
TTATACTCGAGCAAATACTC+AGG - chr4.1:13047458-13047477 None:intergenic 35.0%
TTCCAATCTAGCATCTTCAT+AGG - chr4.1:13046704-13046723 None:intergenic 35.0%
TTGAAAGTGGATGATTCTGA+AGG - chr4.1:13046195-13046214 None:intergenic 35.0%
! AATCAAAGTCAAAGGAGTGT+TGG - chr4.1:13046332-13046351 None:intergenic 35.0%
! AATGATCTTTTGTCTCCAAG+TGG + chr4.1:13047746-13047765 MS.gene27856:CDS 35.0%
! ACTAACACAAGCTTTTGTAG+TGG - chr4.1:13045885-13045904 None:intergenic 35.0%
! ATTCTGAAGGCGCATAATTA+AGG - chr4.1:13046182-13046201 None:intergenic 35.0%
! CTCTAAGAACTTTGACACTT+CGG - chr4.1:13047835-13047854 None:intergenic 35.0%
! CTTCCAACAATTGTTGTTGT+TGG + chr4.1:13046070-13046089 MS.gene27856:CDS 35.0%
! TTCTGAAGGCGCATAATTAA+GGG - chr4.1:13046181-13046200 None:intergenic 35.0%
! TTTAGCTCAGAAGCTTGTTT+AGG + chr4.1:13046792-13046811 MS.gene27856:intron 35.0%
!! TGGTTGATAAAACTGGTTTG+AGG + chr4.1:13046557-13046576 MS.gene27856:CDS 35.0%
!!! AAGTCAGGCTTTTGATTTGA+AGG + chr4.1:13047607-13047626 MS.gene27856:CDS 35.0%
ACAAGCTTCTGAGCTAAAAC+AGG - chr4.1:13046790-13046809 None:intergenic 40.0%
AGATCAACCATGGTAAGATC+AGG - chr4.1:13046373-13046392 None:intergenic 40.0%
AGCTAAAGATTCAAGGACAC+TGG - chr4.1:13046116-13046135 None:intergenic 40.0%
AGTCTCACTCACTTCTTTCT+AGG + chr4.1:13046740-13046759 MS.gene27856:CDS 40.0%
AGTGTCCTTGAATCTTTAGC+TGG + chr4.1:13046115-13046134 MS.gene27856:CDS 40.0%
ATAGCTACTGAAGAGATTGC+TGG + chr4.1:13046289-13046308 MS.gene27856:CDS 40.0%
ATTGGTGCAACAACAGAAAG+AGG - chr4.1:13045962-13045981 None:intergenic 40.0%
CAACAGCTTCCATAACATGT+TGG - chr4.1:13047403-13047422 None:intergenic 40.0%
CATAGGTCTCATCTCCAATT+CGG - chr4.1:13046687-13046706 None:intergenic 40.0%
CTGTTGCAAGAATTATGGAC+AGG + chr4.1:13047867-13047886 MS.gene27856:CDS 40.0%
CTTACTGGAAGATTGTTCTG+AGG + chr4.1:13047642-13047661 MS.gene27856:CDS 40.0%
GACCTATGAAGATGCTAGAT+TGG + chr4.1:13046699-13046718 MS.gene27856:CDS 40.0%
GCAAGAATTATGGACAGGAT+TGG + chr4.1:13047872-13047891 MS.gene27856:CDS 40.0%
GGAGACTGTTGCAAGAATTA+TGG + chr4.1:13047862-13047881 MS.gene27856:CDS 40.0%
GTCTATCAGGATGTCACAAA+TGG + chr4.1:13046537-13046556 MS.gene27856:CDS 40.0%
GTTGTTGGAGATCAATCTTC+AGG + chr4.1:13046085-13046104 MS.gene27856:CDS 40.0%
TCCCTCTTTCCAGAAATAGA+CGG - chr4.1:13047803-13047822 None:intergenic 40.0%
TGGAAGCTGAATTCCTTCTT+TGG - chr4.1:13046056-13046075 None:intergenic 40.0%
TGTTATGGAAGCTGTTGAGA+TGG + chr4.1:13047406-13047425 MS.gene27856:CDS 40.0%
TTGGCATAGACCAATCCATT+TGG - chr4.1:13047157-13047176 None:intergenic 40.0%
! ACTGCTGATGACGTTAACAT+CGG + chr4.1:13046637-13046656 MS.gene27856:CDS 40.0%
! TAAAATCAACAGTGGCGGAA+AGG - chr4.1:13046507-13046526 None:intergenic 40.0%
!! AAGAGTTCTGGCTTTGAAAG+TGG - chr4.1:13046208-13046227 None:intergenic 40.0%
!! AGAGATTAAGGTGTTGGAAG+AGG + chr4.1:13047131-13047150 MS.gene27856:CDS 40.0%
!! TCCGTCTATTTCTGGAAAGA+GGG + chr4.1:13047799-13047818 MS.gene27856:CDS 40.0%
!!! AGGCTTTTGATTTGAAGGCT+AGG + chr4.1:13047612-13047631 MS.gene27856:CDS 40.0%
AAAGCCTGACTTAGCACATG+AGG - chr4.1:13047599-13047618 None:intergenic 45.0%
AAGCCTGACTTAGCACATGA+GGG - chr4.1:13047598-13047617 None:intergenic 45.0%
AATGCATGTGCTTGTCCTCA+GGG - chr4.1:13047020-13047039 None:intergenic 45.0%
ACCTTCTCCAACAATCCTTC+AGG - chr4.1:13046616-13046635 None:intergenic 45.0%
AGGCTAGGATGATTGCTTAC+TGG + chr4.1:13047627-13047646 MS.gene27856:CDS 45.0%
CCAACACATGTGAAGCTTGA+AGG + chr4.1:13047536-13047555 MS.gene27856:CDS 45.0%
CCTTCAAGCTTCACATGTGT+TGG - chr4.1:13047539-13047558 None:intergenic 45.0%
CGTCTCAACATTGCCAAAGA+AGG + chr4.1:13046040-13046059 MS.gene27856:CDS 45.0%
CTTACCATGGTTGATCTTCC+AGG + chr4.1:13046376-13046395 MS.gene27856:CDS 45.0%
GAAGAGTCTCCGTCTATTTC+TGG + chr4.1:13047791-13047810 MS.gene27856:CDS 45.0%
GAGACGCCTTAGATTCTCAA+GGG - chr4.1:13046026-13046045 None:intergenic 45.0%
GATACCTGGAAGATCAACCA+TGG - chr4.1:13046383-13046402 None:intergenic 45.0%
GATGACGTTAACATCGGTCT+AGG + chr4.1:13046643-13046662 MS.gene27856:CDS 45.0%
GTTGACCATGAACAGGTACA+CGG - chr4.1:13046408-13046427 None:intergenic 45.0%
TCCTGAAGGATTGTTGGAGA+AGG + chr4.1:13046612-13046631 MS.gene27856:CDS 45.0%
TGAGACGCCTTAGATTCTCA+AGG - chr4.1:13046027-13046046 None:intergenic 45.0%
TGGTGTTCCTGATCTTACCA+TGG + chr4.1:13046363-13046382 MS.gene27856:CDS 45.0%
TTACCCTCATGTGCTAAGTC+AGG + chr4.1:13047592-13047611 MS.gene27856:CDS 45.0%
TTGACCATGAACAGGTACAC+GGG - chr4.1:13046407-13046426 None:intergenic 45.0%
TTGGAAGAGGCCAAATGGAT+TGG + chr4.1:13047144-13047163 MS.gene27856:CDS 45.0%
! CATGTGAAGCTTGAAGGTGT+TGG + chr4.1:13047542-13047561 MS.gene27856:CDS 45.0%
! GATCTTTTGTCTCCAAGTGG+TGG + chr4.1:13047749-13047768 MS.gene27856:CDS 45.0%
! TTTTGTCTCCAAGTGGTGGT+GGG + chr4.1:13047753-13047772 MS.gene27856:CDS 45.0%
!! ACTGGAAGATTGTTCTGAGG+AGG + chr4.1:13047645-13047664 MS.gene27856:CDS 45.0%
!! CTCCGTCTATTTCTGGAAAG+AGG + chr4.1:13047798-13047817 MS.gene27856:CDS 45.0%
!! TGATTTCGAAGAGTACCCTG+AGG + chr4.1:13047002-13047021 MS.gene27856:CDS 45.0%
!! TTGTCAGGTTGACCATGAAC+AGG - chr4.1:13046415-13046434 None:intergenic 45.0%
ATCACCCGTGTACCTGTTCA+TGG + chr4.1:13046400-13046419 MS.gene27856:CDS 50.0%
CAATGCATGTGCTTGTCCTC+AGG - chr4.1:13047021-13047040 None:intergenic 50.0%
GTCTGTGTGCGAAACCGAAT+TGG + chr4.1:13046670-13046689 MS.gene27856:CDS 50.0%
TCGACGCCCTTGAGAATCTA+AGG + chr4.1:13046017-13046036 MS.gene27856:CDS 50.0%
TGGACAAGGAATCTGCACGA+GGG + chr4.1:13046153-13046172 MS.gene27856:CDS 50.0%
! CTTTTGTCTCCAAGTGGTGG+TGG + chr4.1:13047752-13047771 MS.gene27856:CDS 50.0%
! GAGGTTGAGCTTGCTCAACA+TGG - chr4.1:13045943-13045962 None:intergenic 50.0%
!! AGATTGCTGGTTCTGCCAGA+GGG + chr4.1:13046302-13046321 MS.gene27856:CDS 50.0%
!! AGGAGTGTTGGAAATCCCTC+TGG - chr4.1:13046320-13046339 None:intergenic 50.0%
!! AGGTGTTGGAAGAGGCCAAA+TGG + chr4.1:13047139-13047158 MS.gene27856:CDS 50.0%
ACAGGTACACGGGTGATACC+TGG - chr4.1:13046397-13046416 None:intergenic 55.0%
ATCAGCTTGCCACGTGGACA+AGG + chr4.1:13046139-13046158 MS.gene27856:CDS 55.0%
CTCTCAATCCCACCACCACT+TGG - chr4.1:13047764-13047783 None:intergenic 55.0%
GTGCAGATTCCTTGTCCACG+TGG - chr4.1:13046151-13046170 None:intergenic 55.0%
GTGGACAAGGAATCTGCACG+AGG + chr4.1:13046152-13046171 MS.gene27856:CDS 55.0%
!! CAAGTGGTGGTGGGATTGAG+AGG + chr4.1:13047762-13047781 MS.gene27856:CDS 55.0%
!! GAGATTGCTGGTTCTGCCAG+AGG + chr4.1:13046301-13046320 MS.gene27856:CDS 55.0%
!! TTCTCAAGGGCGTCGAGAAC+TGG - chr4.1:13046013-13046032 None:intergenic 55.0%
CAGGTTTCCACTCGCAGAGC+AGG + chr4.1:13047341-13047360 MS.gene27856:CDS 60.0%
GCTGGTATCAGCTTGCCACG+TGG + chr4.1:13046133-13046152 MS.gene27856:CDS 60.0%
TTTCTCGCCTGCTCTGCGAG+TGG - chr4.1:13047351-13047370 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13045860 13047907 13045860 ID=MS.gene27856
chr4.1 mRNA 13045860 13047907 13045860 ID=MS.gene27856.t1;Parent=MS.gene27856
chr4.1 exon 13045860 13046781 13045860 ID=MS.gene27856.t1.exon1;Parent=MS.gene27856.t1
chr4.1 CDS 13045860 13046781 13045860 ID=cds.MS.gene27856.t1;Parent=MS.gene27856.t1
chr4.1 exon 13046848 13047165 13046848 ID=MS.gene27856.t1.exon2;Parent=MS.gene27856.t1
chr4.1 CDS 13046848 13047165 13046848 ID=cds.MS.gene27856.t1;Parent=MS.gene27856.t1
chr4.1 exon 13047294 13047907 13047294 ID=MS.gene27856.t1.exon3;Parent=MS.gene27856.t1
chr4.1 CDS 13047294 13047907 13047294 ID=cds.MS.gene27856.t1;Parent=MS.gene27856.t1
Gene Sequence

>MS.gene27856

ATGGTTGTGACTTCAAAGAGAACCACTACAAAAGCTTGTGTTAGTGCTAACCAACGAGATGAATCATCACTTTCATTAGTCCATGTTGAGCAAGCTCAACCTCTTTCTGTTGTTGCACCAATTGTTTCATCCTACAATGAAAAAATACGTCCAGTTCTCGACGCCCTTGAGAATCTAAGGCGTCTCAACATTGCCAAAGAAGGAATTCAGCTTCCAACAATTGTTGTTGTTGGAGATCAATCTTCAGGAAAGTCCAGTGTCCTTGAATCTTTAGCTGGTATCAGCTTGCCACGTGGACAAGGAATCTGCACGAGGGTACCCTTAATTATGCGCCTTCAGAATCATCCACTTTCAAAGCCAGAACTCTTGTTAGAATTCAATGGCAAGACAGTTTTCACTGATGAAAAAGATGTTTCTGATGCAATAAACATAGCTACTGAAGAGATTGCTGGTTCTGCCAGAGGGATTTCCAACACTCCTTTGACTTTGATTGTCAAGAAAAATGGTGTTCCTGATCTTACCATGGTTGATCTTCCAGGTATCACCCGTGTACCTGTTCATGGTCAACCTGACAATATCTATGATCAGATAAAAGATATTATTATGGAGTATATAACTCCTGAAGAAAGTATTATCTTGAATGTCCTTTCCGCCACTGTTGATTTTACTACTTGTGAGTCTATCAGGATGTCACAAATGGTTGATAAAACTGGTTTGAGGACACTAGCTGTTGTGACAAAAGCAGATAAATCTCCTGAAGGATTGTTGGAGAAGGTTACTGCTGATGACGTTAACATCGGTCTAGGTTATGTCTGTGTGCGAAACCGAATTGGAGATGAGACCTATGAAGATGCTAGATTGGAAGAAGAAAAGCTGTTCGAGTCTCACTCACTTCTTTCTAGGATTGACAAATCCATTGTTGGTATTCCTGTTTTAGCTCAGAAGCTTGTTTAGGTTCAAGCTATGATTATCTCAAAAACTTTGCCAGAGATCATCAAGAAGATTAATGAGAAGCTTGCTTCTAATGCAAATGAGTTAGAGAATTTACCTGCTAATTTATCTTCTGTTGCTGATGCTATGACAGCTTTTATGCATATTCTTGGTTTGTCTAGAGATTCTCTCAAAAAGATTCTTCTTACAGGTGATTTCGAAGAGTACCCTGAGGACAAGCACATGCATTGCACTGCTCGATTAGTTGAGATGCTTAACTTATATGCAAGCGATCTTGAAAACTGTGCTGAAAGTGATGCGAAAAAGAATTTTCTTATGGAAGAGATTAAGGTGTTGGAAGAGGCCAAATGGATTGGTCTATGCCAAGAACTGCATTTTTGACAATACTACTGCATTTTTGACAATACTGCAGAGAAAAGTTAAGGGCAACATGTTATGGAAGTTCTAACTTTGTTGATAATGTTTGGAACTATCTTGAATCAGTTGTGATTTCTATTTTGAATCATCATTCTTCAAATTATTATCAGCTTCAGGTTTCCACTCGCAGAGCAGGCGAGAAATTGATTGCTAAGAAGAAGAAAAGTTCAGTCCAACATGTTATGGAAGCTGTTGAGATGGAAAAACTGACAGATTACACTTGTAATCCTGAGTATTTGCTCGAGTATAATAGGCTGATAGCTCAACAAGAAGCGTTTGTGAAAGAAGTTCTAAGCAATGAAACAAAACCAACACATGTGAAGCTTGAAGGTGTTGGTGAAATTGATGTTGTTAATCTGAGGAATTACCCTCATGTGCTAAGTCAGGCTTTTGATTTGAAGGCTAGGATGATTGCTTACTGGAAGATTGTTCTGAGGAGGCTCATTGACAGCATTGCTCTGCACTTGATGCTAAGCATCAATGAACTGATTAACAATGATTTGCAGAAGGAGATTTGTAATGATCTTTTGTCTCCAAGTGGTGGTGGGATTGAGAGGCTGCTTGAAGAGTCTCCGTCTATTTCTGGAAAGAGGGAGAAGCTAAGCCGAAGTGTCAAAGTTCTTAGAGAAAGTAAGGAGACTGTTGCAAGAATTATGGACAGGATTGGAATCTATGATTAG
Protein sequence

>MS.gene27856.t1

MVVTSKRTTTKACVSANQRDESSLSLVHVEQAQPLSVVAPIVSSYNEKIRPVLDALENLRRLNIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLIMRLQNHPLSKPELLLEFNGKTVFTDEKDVSDAINIATEEIAGSARGISNTPLTLIVKKNGVPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFTTCESIRMSQMVDKTGLRTLAVVTKADKSPEGLLEKVTADDVNIGLGYVCVRNRIGDETYEDARLEEEKLFESHSLLSRIDKSIVEIIKKINEKLASNANELENLPANLSSVADAMTAFMHILGLSRDSLKKILLTGDFEEYPEDKHMHCTARLVEMLNLYASDLENCAESDAKKNFLMEEIKVLEEAKWIVVISILNHHSSNYYQLQVSTRRAGEKLIAKKKKSSVQHVMEAVEMEKLTDYTCNPEYLLEYNRLIAQQEAFVKEVLSNETKPTHVKLEGVGEIDVVNLRNYPHVLSQAFDLKARMIAYWKIVLRRLIDSIALHLMLSINELINNDLQKEICNDLLSPSGGGIERLLEESPSISGKREKLSRSVKVLRESKETVARIMDRIGIYD