AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27857

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27857.t1 MTR_8g087770 85.373 670 92 3 1 669 1 665 0.0 1152
MS.gene27857.t1 MTR_8g087750 82.406 665 112 3 6 669 11 671 0.0 1121
MS.gene27857.t1 MTR_8g087763 81.858 226 40 1 373 598 4 228 3.46e-131 391
MS.gene27857.t1 MTR_4g093750 33.666 401 241 10 66 451 35 425 8.08e-44 168
MS.gene27857.t1 MTR_4g093750 39.373 287 154 7 66 338 35 315 6.23e-43 166
MS.gene27857.t1 MTR_2g009690 38.246 285 164 6 62 336 40 322 3.56e-40 158
MS.gene27857.t1 MTR_5g009150 28.726 463 304 12 68 517 35 484 1.80e-34 139
MS.gene27857.t1 MTR_1g032550 34.062 320 179 10 68 368 35 341 3.08e-34 139
MS.gene27857.t1 MTR_8g105580 33.758 314 187 7 68 368 35 340 7.47e-34 137
MS.gene27857.t1 MTR_8g105580 33.758 314 187 7 68 368 35 340 9.93e-34 137
MS.gene27857.t1 MTR_8g009510 35.202 321 174 11 68 368 40 346 2.36e-33 136
MS.gene27857.t1 MTR_7g451010 34.688 320 177 10 68 368 40 346 1.52e-32 134
MS.gene27857.t1 MTR_4g098920 34.169 319 180 9 68 368 34 340 2.33e-31 130
MS.gene27857.t1 MTR_8g099550 32.803 314 191 7 68 368 34 340 5.08e-31 129
MS.gene27857.t1 MTR_8g027675 41.176 187 105 3 68 251 34 218 7.56e-31 128
MS.gene27857.t1 MTR_4g085860 73.684 76 19 1 205 279 2 77 6.33e-30 113
MS.gene27857.t1 MTR_6g045107 72.368 76 20 1 205 279 2 77 5.73e-28 107
MS.gene27857.t1 MTR_8g009510 31.474 251 142 8 135 368 2 239 1.99e-18 89.7
MS.gene27857.t1 MTR_8g463270 29.907 214 139 4 64 268 42 253 1.03e-16 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27857.t1 AT1G60500 59.027 637 258 2 34 669 31 665 0.0 784
MS.gene27857.t1 AT1G60530 71.379 290 75 1 17 306 19 300 1.58e-142 419
MS.gene27857.t1 AT2G14120 29.505 444 290 11 24 451 1 437 7.21e-39 154
MS.gene27857.t1 AT2G14120 33.234 337 207 7 24 345 1 334 8.07e-39 154
MS.gene27857.t1 AT2G14120 29.505 444 290 11 24 451 1 437 8.88e-39 154
MS.gene27857.t1 AT2G14120 29.505 444 290 11 24 451 1 437 1.01e-38 154
MS.gene27857.t1 AT4G33650 29.705 441 268 11 35 451 30 452 2.08e-38 153
MS.gene27857.t1 AT4G33650 29.705 441 268 11 35 451 30 452 2.33e-38 152
MS.gene27857.t1 AT1G14830 33.439 314 189 7 68 368 35 341 7.22e-35 140
MS.gene27857.t1 AT5G42080 29.806 463 299 11 68 517 34 483 1.53e-34 140
MS.gene27857.t1 AT5G42080 29.806 463 299 11 68 517 34 483 1.53e-34 140
MS.gene27857.t1 AT3G60190 34.177 316 184 9 68 368 40 346 1.74e-33 137
MS.gene27857.t1 AT2G44590 24.682 628 398 23 68 671 35 611 7.40e-33 134
MS.gene27857.t1 AT5G42080 29.501 461 297 11 68 517 34 477 1.80e-32 133
MS.gene27857.t1 AT5G42080 33.548 310 194 6 68 368 34 340 1.91e-32 130
MS.gene27857.t1 AT3G61760 33.228 316 187 9 68 368 34 340 2.45e-30 125
MS.gene27857.t1 AT3G61760 33.228 316 187 9 68 368 34 340 2.88e-30 126
MS.gene27857.t1 AT3G61760 33.228 316 187 9 68 368 34 340 3.07e-30 127
MS.gene27857.t1 AT2G44590 23.567 628 388 24 68 671 35 594 1.22e-23 106
MS.gene27857.t1 AT2G44590 29.641 334 186 12 68 385 35 335 5.53e-23 104
MS.gene27857.t1 AT1G53140 27.848 237 157 6 43 268 36 269 1.09e-14 78.6

Find 131 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAATAGATTTATCTATTT+TGG 0.051549 4.1:-13043452 None:intergenic
TTAATCTTCTTAATGATTTC+AGG 0.171207 4.1:-13043538 None:intergenic
GGAAAATTTGCCTGCCAATT+TGG 0.204709 4.1:+13043591 MS.gene27857:CDS
CTTGAGGAAGCCAAGATTAT+TGG 0.224459 4.1:+13043838 MS.gene27857:CDS
TTCTTAACAATCAAAGTTAA+AGG 0.234341 4.1:-13043031 None:intergenic
GCTGTTCTTGGCATGAAATT+TGG 0.254803 4.1:-13043865 None:intergenic
GAACTTTGATTTCGTCCATT+AGG 0.265918 4.1:-13043816 None:intergenic
GGGAAGTTGGATTCCCTCTT+TGG 0.273448 4.1:-13042747 None:intergenic
TGACAGTGTTTGGAACTATT+TGG 0.274905 4.1:+13043948 MS.gene27857:CDS
GAGGAGTCTCCTTCAATATC+AGG 0.303692 4.1:+13044477 MS.gene27857:CDS
TTACTACTTGTGAGTCCATT+AGG 0.307685 4.1:+13043218 MS.gene27857:CDS
GCCTGCCAATTTGGCATCAT+TGG 0.308754 4.1:+13043600 MS.gene27857:CDS
CAAAGCACCAGAAGGCTTGC+TGG 0.312662 4.1:+13043300 MS.gene27857:CDS
ATTTGATCATAGATATTATC+AGG 0.327362 4.1:-13043121 None:intergenic
TATTGTAGGAATTCCTGTTT+TGG 0.327596 4.1:+13043468 MS.gene27857:CDS
TTCAAGATAATACTTTCTTC+TGG 0.339329 4.1:-13043172 None:intergenic
ATCAGCCAATGATGCCAAAT+TGG 0.340224 4.1:-13043605 None:intergenic
AATGTAAGAAAAGCTGTTCT+TGG 0.343865 4.1:-13043877 None:intergenic
TAATATCTATGATCAAATTA+AGG 0.347385 4.1:+13043126 MS.gene27857:CDS
TTTGTGTAAGGAATAGAATT+GGG 0.352743 4.1:+13043365 MS.gene27857:CDS
TCTTAACAATCAAAGTTAAA+GGG 0.360721 4.1:-13043030 None:intergenic
AATGATTTCAGGCAAAGTTT+TGG 0.361226 4.1:-13043527 None:intergenic
TTAACCATGGTTGATCTTCC+AGG 0.367637 4.1:+13043070 MS.gene27857:CDS
CTTCCCACCATTGTTGTTGT+TGG 0.371021 4.1:+13042764 MS.gene27857:CDS
ACACTGTCAACAAAATTAAC+TGG 0.371215 4.1:-13043934 None:intergenic
TCACAAACTGTTGATAAAAC+TGG 0.375821 4.1:+13043244 MS.gene27857:CDS
GTTTGTGTAAGGAATAGAAT+TGG 0.375877 4.1:+13043364 MS.gene27857:CDS
AAAATAGATAAATCTATTGT+AGG 0.376484 4.1:+13043454 MS.gene27857:CDS
GACTCAGGCCTTTGACTTGA+AGG 0.382103 4.1:+13044293 MS.gene27857:CDS
ATTTGGAAGACCAATAATCT+TGG 0.383624 4.1:-13043848 None:intergenic
AGATCAACCATGGTTAAATC+TGG 0.385650 4.1:-13043064 None:intergenic
GGCTAATAGTTTACATGAGT+TGG 0.395161 4.1:+13043570 MS.gene27857:CDS
TCATTGTAAGATGATACTAT+TGG 0.397762 4.1:-13042671 None:intergenic
GTCTTGGTTATGTTTGTGTA+AGG 0.401697 4.1:+13043353 MS.gene27857:CDS
TGTCTCTTCCCTGATATTGA+AGG 0.410432 4.1:-13044486 None:intergenic
CATTACTTCAGAGGAATGTA+AGG 0.420635 4.1:+13043896 MS.gene27857:CDS
CAAGAGTACCATTGGTTATG+AGG 0.425077 4.1:+13042864 MS.gene27857:CDS
AACAGTTTGTGACATCCTAA+TGG 0.425745 4.1:-13043233 None:intergenic
ACAAGCTTCTGTGCCAAAAC+AGG 0.426561 4.1:-13043481 None:intergenic
ACAGCCACTGAGGAGCTTGC+TGG 0.430442 4.1:+13042983 MS.gene27857:CDS
TCAAGTACTGTGAATCTTGA+AGG 0.433218 4.1:+13044219 MS.gene27857:CDS
CAATGCATTTGCTTATCATC+AGG 0.438737 4.1:-13043712 None:intergenic
TAAGAAGATTAATGAGAAGT+TGG 0.440046 4.1:+13043549 MS.gene27857:CDS
CCTCAGCACAATCTTCCAAT+AGG 0.443004 4.1:-13044328 None:intergenic
GCATTGCACCGCTAGATTAG+TGG 0.443244 4.1:+13043729 MS.gene27857:CDS
ACCAGAAGGCTTGCTGGAAA+AGG 0.448222 4.1:+13043306 MS.gene27857:CDS
AGGCAAGGCTAATTGCCTAT+TGG 0.448827 4.1:+13044313 MS.gene27857:CDS
ATGTAACCCTGAGTACTTGC+AGG 0.450401 4.1:+13044131 MS.gene27857:CDS
ACAAGCTCTGGCTGTGGAAG+TGG 0.452099 4.1:-13042899 None:intergenic
TTATACTCCTGCAAGTACTC+AGG 0.459259 4.1:-13044138 None:intergenic
GGAAGAGACAGAAGTTGAGC+AGG 0.460748 4.1:+13044499 MS.gene27857:CDS
AGGGGTGTTGGAAATCCCTT+TGG 0.462637 4.1:-13043011 None:intergenic
CAACAACAATGGTGGGAAGT+TGG 0.467309 4.1:-13042760 None:intergenic
GATGATGTTAACATCGGTCT+TGG 0.467639 4.1:+13043337 MS.gene27857:CDS
AACAAAACAGACATGACAAA+AGG 0.469116 4.1:-13044606 None:intergenic
TTCGTCCATTAGGAAATCCT+TGG 0.470036 4.1:-13043806 None:intergenic
TTATCAGGTTGGCCATGTAC+AGG 0.471735 4.1:-13043106 None:intergenic
GAGCTTGCTGGCACTGCCAA+AGG 0.478600 4.1:+13042995 MS.gene27857:CDS
GTACTCTTGTGCAAATTCCT+TGG 0.481903 4.1:-13042850 None:intergenic
GCCAATGATGCCAAATTGGC+AGG 0.486397 4.1:-13043601 None:intergenic
TATGACCAAGGATTTCCTAA+TGG 0.487515 4.1:+13043801 MS.gene27857:CDS
TTATACTCTAAAACAAGCTC+TGG 0.488227 4.1:-13042911 None:intergenic
AATCAAAGTTAAAGGGGTGT+TGG 0.500893 4.1:-13043023 None:intergenic
TGTGCTGCAAGGTGTTGAAA+TGG 0.500919 4.1:+13044089 MS.gene27857:CDS
TGCAAACTATTATCAGCTGC+AGG 0.502339 4.1:+13044005 MS.gene27857:CDS
CTTGAATGTTCTTTCTGCCA+CGG 0.502379 4.1:+13043189 MS.gene27857:CDS
TCAGCATTTGTTGTTGACTC+AGG 0.505197 4.1:+13044278 MS.gene27857:CDS
ATTACTCGTGTCCCTGTACA+TGG 0.509421 4.1:+13043094 MS.gene27857:CDS
GTTGTTGGTGATCAATCGTC+TGG 0.509934 4.1:+13042779 MS.gene27857:CDS
GATCATAGATATTATCAGGT+TGG 0.512244 4.1:-13043117 None:intergenic
AATTTGCACAAGAGTACCAT+TGG 0.513388 4.1:+13042856 MS.gene27857:CDS
AGTGTCCTTGAGTCATTAGC+TGG 0.515596 4.1:+13042809 MS.gene27857:CDS
TCAAATTAAGGATATTATCA+TGG 0.518955 4.1:+13043138 MS.gene27857:CDS
ATATAATCATAGCTTGCACT+TGG 0.531392 4.1:-13043504 None:intergenic
AATGAACTGTTATCAGCAAA+TGG 0.537861 4.1:+13044432 MS.gene27857:CDS
ATAAGTTTGCCACGTGGCCA+AGG 0.541062 4.1:+13042833 MS.gene27857:CDS
TGAAGAATCATCACTTGCAT+TGG 0.541598 4.1:+13042604 MS.gene27857:CDS
AGCTTGCTGGCACTGCCAAA+GGG 0.547647 4.1:+13042996 MS.gene27857:CDS
CTTAACAATCAAAGTTAAAG+GGG 0.548616 4.1:-13043029 None:intergenic
TCTAAAACAAGCTCTGGCTG+TGG 0.552834 4.1:-13042905 None:intergenic
GGACGAAATCAAAGTTCTTG+AGG 0.554038 4.1:+13043822 MS.gene27857:CDS
AACTAAAACAAACATAATGT+TGG 0.557553 4.1:-13044559 None:intergenic
TAGCCTTGCCTTCAAGTCAA+AGG 0.560724 4.1:-13044301 None:intergenic
TGGCAATGTTGATACGCCGT+AGG 0.566250 4.1:-13042727 None:intergenic
ACAGGGACACGAGTAATGCC+TGG 0.569922 4.1:-13043088 None:intergenic
ATCACCAACAACAACAATGG+TGG 0.572674 4.1:-13042768 None:intergenic
AACAAACATAATGTTGGCCA+CGG 0.573159 4.1:-13044553 None:intergenic
CGTATCAACATTGCCAAAGA+GGG 0.575382 4.1:+13042734 MS.gene27857:CDS
AGTGCCAGCAAGCTCCTCAG+TGG 0.578957 4.1:-13042987 None:intergenic
TATACTCCTGCAAGTACTCA+GGG 0.580347 4.1:-13044137 None:intergenic
TGTGAATGTTGATCTGCAAA+AGG 0.585319 4.1:+13044401 MS.gene27857:CDS
AGGAGTCTCCTTCAATATCA+GGG 0.586223 4.1:+13044478 MS.gene27857:CDS
AGCTAATGACTCAAGGACAC+TGG 0.593764 4.1:-13042807 None:intergenic
ATTGGAAGATTGTGCTGAGG+AGG 0.597029 4.1:+13044331 MS.gene27857:CDS
ATTACTTCAGAGGAATGTAA+GGG 0.598482 4.1:+13043897 MS.gene27857:CDS
TTATACCAGCTAATGACTCA+AGG 0.606048 4.1:-13042814 None:intergenic
TATCAGGTTGGCCATGTACA+GGG 0.611782 4.1:-13043105 None:intergenic
CAAGCAAGTTTCAACTGATG+AGG 0.612510 4.1:+13042937 MS.gene27857:CDS
GCGTATCAACATTGCCAAAG+AGG 0.613041 4.1:+13042733 MS.gene27857:CDS
TCTATTCAACATGTGCTGCA+AGG 0.615822 4.1:+13044078 MS.gene27857:CDS
TAGATGCCCTAGAAAACCTA+CGG 0.617872 4.1:+13042711 MS.gene27857:CDS
TGGTGTTGAGAGGCTGCTTG+AGG 0.618613 4.1:+13044458 MS.gene27857:CDS
TCACCAACAACAACAATGGT+GGG 0.623546 4.1:-13042767 None:intergenic
AGGCCTTTGACTTGAAGGCA+AGG 0.625900 4.1:+13044298 MS.gene27857:CDS
CTGTTATCAGCAAATGGTGG+TGG 0.626367 4.1:+13044438 MS.gene27857:CDS
GTGCAAATTCCTTGGCCACG+TGG 0.626948 4.1:-13042842 None:intergenic
TGGTATTCCAGATTTAACCA+TGG 0.634354 4.1:+13043057 MS.gene27857:CDS
TTGATCACCAACAACAACAA+TGG 0.636905 4.1:-13042771 None:intergenic
CAAATGGTGGTGGTGTTGAG+AGG 0.637671 4.1:+13044448 MS.gene27857:CDS
TTGTGTAAGGAATAGAATTG+GGG 0.639370 4.1:+13043366 MS.gene27857:CDS
GCTCTGGCTGTGGAAGTGGA+TGG 0.648549 4.1:-13042895 None:intergenic
GGTGCAACAACAAGTGGTTG+AGG 0.648777 4.1:-13042650 None:intergenic
AAAGCTGACAAAGCACCAGA+AGG 0.649435 4.1:+13043292 MS.gene27857:CDS
AATTTGCAGAAAAGCAGACA+TGG 0.650037 4.1:-13043629 None:intergenic
TGCTGAAAGTAATATGACCA+AGG 0.653435 4.1:+13043789 MS.gene27857:CDS
GAGAGAAAGCAAAGAGACCG+TGG 0.654345 4.1:+13044536 MS.gene27857:CDS
CAACATATCCACTAATCTAG+CGG 0.654700 4.1:-13043737 None:intergenic
ACTATTGGTGCAACAACAAG+TGG 0.655043 4.1:-13042656 None:intergenic
TGCTATAAACACAGCCACTG+AGG 0.658388 4.1:+13042973 MS.gene27857:CDS
ACCGCTGATGATGTTAACAT+CGG 0.662734 4.1:+13043331 MS.gene27857:CDS
ACCGATGTTAACATCATCAG+CGG 0.666783 4.1:-13043332 None:intergenic
CTGTTGATAAAACTGGTCTG+AGG 0.690962 4.1:+13043251 MS.gene27857:CDS
TTACTTCAGAGGAATGTAAG+GGG 0.701485 4.1:+13043898 MS.gene27857:CDS
AATGCCTGGAAGATCAACCA+TGG 0.705380 4.1:-13043074 None:intergenic
CCTATTGGAAGATTGTGCTG+AGG 0.716472 4.1:+13044328 MS.gene27857:CDS
GTTGATCATCAACATCAACA+TGG 0.719635 4.1:-13042628 None:intergenic
ACAAGTAGTAAAATCAACCG+TGG 0.733510 4.1:-13043206 None:intergenic
GCTGATAATAGTTTGCAGAG+TGG 0.741343 4.1:-13043999 None:intergenic
GAACTGTTATCAGCAAATGG+TGG 0.747464 4.1:+13044435 MS.gene27857:CDS
GCTGGTATAAGTTTGCCACG+TGG 0.749894 4.1:+13042827 MS.gene27857:CDS
ACTGTGAATCTTGAAGGACA+TGG 0.765829 4.1:+13044225 MS.gene27857:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGATAAATCTATTGT+AGG + chr4.1:13043454-13043473 MS.gene27857:CDS 15.0%
!! TAATATCTATGATCAAATTA+AGG + chr4.1:13043126-13043145 MS.gene27857:CDS 15.0%
!!! TACAATAGATTTATCTATTT+TGG - chr4.1:13043455-13043474 None:intergenic 15.0%
!! AACTAAAACAAACATAATGT+TGG - chr4.1:13044562-13044581 None:intergenic 20.0%
!! ACTTTGATTGTTAAGAAAAA+TGG + chr4.1:13043037-13043056 MS.gene27857:CDS 20.0%
!! ATTTGATCATAGATATTATC+AGG - chr4.1:13043124-13043143 None:intergenic 20.0%
!! TCAAATTAAGGATATTATCA+TGG + chr4.1:13043138-13043157 MS.gene27857:CDS 20.0%
!! TCTTAACAATCAAAGTTAAA+GGG - chr4.1:13043033-13043052 None:intergenic 20.0%
!! TTAAAAAGATTCTTCTGATA+GGG + chr4.1:13043674-13043693 MS.gene27857:CDS 20.0%
!! TTAATCTTCTTAATGATTTC+AGG - chr4.1:13043541-13043560 None:intergenic 20.0%
!! TTCTTAACAATCAAAGTTAA+AGG - chr4.1:13043034-13043053 None:intergenic 20.0%
!!! AATAGAATTCTTTTTCTTCT+TGG - chr4.1:13044064-13044083 None:intergenic 20.0%
! CTTAAAAAGATTCTTCTGAT+AGG + chr4.1:13043673-13043692 MS.gene27857:CDS 25.0%
! CTTAACAATCAAAGTTAAAG+GGG - chr4.1:13043032-13043051 None:intergenic 25.0%
! TAAAAAGATTCTTCTGATAG+GGG + chr4.1:13043675-13043694 MS.gene27857:CDS 25.0%
! TAAGAAGATTAATGAGAAGT+TGG + chr4.1:13043549-13043568 MS.gene27857:CDS 25.0%
! TCATTGTAAGATGATACTAT+TGG - chr4.1:13042674-13042693 None:intergenic 25.0%
! TTCAAGATAATACTTTCTTC+TGG - chr4.1:13043175-13043194 None:intergenic 25.0%
! TTTGTGTAAGGAATAGAATT+GGG + chr4.1:13043365-13043384 MS.gene27857:CDS 25.0%
!! TTAATTTTGTTGACAGTGTT+TGG + chr4.1:13043938-13043957 MS.gene27857:CDS 25.0%
AACAAAACAGACATGACAAA+AGG - chr4.1:13044609-13044628 None:intergenic 30.0%
AATGAACTGTTATCAGCAAA+TGG + chr4.1:13044432-13044451 MS.gene27857:CDS 30.0%
AATGTAAGAAAAGCTGTTCT+TGG - chr4.1:13043880-13043899 None:intergenic 30.0%
ACACTGTCAACAAAATTAAC+TGG - chr4.1:13043937-13043956 None:intergenic 30.0%
ATTACTTCAGAGGAATGTAA+GGG + chr4.1:13043897-13043916 MS.gene27857:CDS 30.0%
ATTTGGAAGACCAATAATCT+TGG - chr4.1:13043851-13043870 None:intergenic 30.0%
GATCATAGATATTATCAGGT+TGG - chr4.1:13043120-13043139 None:intergenic 30.0%
GTTTGTGTAAGGAATAGAAT+TGG + chr4.1:13043364-13043383 MS.gene27857:CDS 30.0%
TTGTGTAAGGAATAGAATTG+GGG + chr4.1:13043366-13043385 MS.gene27857:CDS 30.0%
! AATGATTTCAGGCAAAGTTT+TGG - chr4.1:13043530-13043549 None:intergenic 30.0%
! ATATAATCATAGCTTGCACT+TGG - chr4.1:13043507-13043526 None:intergenic 30.0%
! CTTTTCTTACATTACTTCAG+AGG + chr4.1:13043887-13043906 MS.gene27857:CDS 30.0%
! TATTGTAGGAATTCCTGTTT+TGG + chr4.1:13043468-13043487 MS.gene27857:CDS 30.0%
! TCACAAACTGTTGATAAAAC+TGG + chr4.1:13043244-13043263 MS.gene27857:CDS 30.0%
! TTCACGTTTAGTAGATTTTG+TGG - chr4.1:13042588-13042607 None:intergenic 30.0%
!! TTATACTCTAAAACAAGCTC+TGG - chr4.1:13042914-13042933 None:intergenic 30.0%
!!! GAGCTTGTTTTAGAGTATAA+TGG + chr4.1:13042914-13042933 MS.gene27857:CDS 30.0%
AACAAACATAATGTTGGCCA+CGG - chr4.1:13044556-13044575 None:intergenic 35.0%
AACAGTTTGTGACATCCTAA+TGG - chr4.1:13043236-13043255 None:intergenic 35.0%
AATTTGCAGAAAAGCAGACA+TGG - chr4.1:13043632-13043651 None:intergenic 35.0%
ACAAGTAGTAAAATCAACCG+TGG - chr4.1:13043209-13043228 None:intergenic 35.0%
AGATCAACCATGGTTAAATC+TGG - chr4.1:13043067-13043086 None:intergenic 35.0%
CAACATATCCACTAATCTAG+CGG - chr4.1:13043740-13043759 None:intergenic 35.0%
CAATGCATTTGCTTATCATC+AGG - chr4.1:13043715-13043734 None:intergenic 35.0%
CATTACTTCAGAGGAATGTA+AGG + chr4.1:13043896-13043915 MS.gene27857:CDS 35.0%
GGCTAATAGTTTACATGAGT+TGG + chr4.1:13043570-13043589 MS.gene27857:CDS 35.0%
GTCTTGGTTATGTTTGTGTA+AGG + chr4.1:13043353-13043372 MS.gene27857:CDS 35.0%
GTTGATCATCAACATCAACA+TGG - chr4.1:13042631-13042650 None:intergenic 35.0%
TATGACCAAGGATTTCCTAA+TGG + chr4.1:13043801-13043820 MS.gene27857:CDS 35.0%
TGAAGAATCATCACTTGCAT+TGG + chr4.1:13042604-13042623 MS.gene27857:CDS 35.0%
TGCTGAAAGTAATATGACCA+AGG + chr4.1:13043789-13043808 MS.gene27857:CDS 35.0%
TGGTATTCCAGATTTAACCA+TGG + chr4.1:13043057-13043076 MS.gene27857:CDS 35.0%
TGTGAATGTTGATCTGCAAA+AGG + chr4.1:13044401-13044420 MS.gene27857:CDS 35.0%
TTACTACTTGTGAGTCCATT+AGG + chr4.1:13043218-13043237 MS.gene27857:CDS 35.0%
TTACTTCAGAGGAATGTAAG+GGG + chr4.1:13043898-13043917 MS.gene27857:CDS 35.0%
TTATACCAGCTAATGACTCA+AGG - chr4.1:13042817-13042836 None:intergenic 35.0%
TTGATCACCAACAACAACAA+TGG - chr4.1:13042774-13042793 None:intergenic 35.0%
! AATCAAAGTTAAAGGGGTGT+TGG - chr4.1:13043026-13043045 None:intergenic 35.0%
! GAACTTTGATTTCGTCCATT+AGG - chr4.1:13043819-13043838 None:intergenic 35.0%
! GAAGAAGAGAAGCTTTTTGA+GGG + chr4.1:13043415-13043434 MS.gene27857:CDS 35.0%
! TCAAGTACTGTGAATCTTGA+AGG + chr4.1:13044219-13044238 MS.gene27857:CDS 35.0%
! TGAAGAAGAGAAGCTTTTTG+AGG + chr4.1:13043414-13043433 MS.gene27857:CDS 35.0%
! TGACAGTGTTTGGAACTATT+TGG + chr4.1:13043948-13043967 MS.gene27857:CDS 35.0%
!! AATTTGCACAAGAGTACCAT+TGG + chr4.1:13042856-13042875 MS.gene27857:CDS 35.0%
!!! TGTCTGTTTTGTTTGTCTGT+TGG + chr4.1:13044616-13044635 MS.gene27857:CDS 35.0%
ACCGATGTTAACATCATCAG+CGG - chr4.1:13043335-13043354 None:intergenic 40.0%
ACTATTGGTGCAACAACAAG+TGG - chr4.1:13042659-13042678 None:intergenic 40.0%
ACTGTGAATCTTGAAGGACA+TGG + chr4.1:13044225-13044244 MS.gene27857:CDS 40.0%
AGGAGTCTCCTTCAATATCA+GGG + chr4.1:13044478-13044497 MS.gene27857:CDS 40.0%
ATCACCAACAACAACAATGG+TGG - chr4.1:13042771-13042790 None:intergenic 40.0%
ATCAGCCAATGATGCCAAAT+TGG - chr4.1:13043608-13043627 None:intergenic 40.0%
CAAGCAAGTTTCAACTGATG+AGG + chr4.1:13042937-13042956 MS.gene27857:CDS 40.0%
CGTATCAACATTGCCAAAGA+GGG + chr4.1:13042734-13042753 MS.gene27857:CDS 40.0%
CTTGAATGTTCTTTCTGCCA+CGG + chr4.1:13043189-13043208 MS.gene27857:CDS 40.0%
CTTGAGGAAGCCAAGATTAT+TGG + chr4.1:13043838-13043857 MS.gene27857:CDS 40.0%
GAACTGTTATCAGCAAATGG+TGG + chr4.1:13044435-13044454 MS.gene27857:CDS 40.0%
GATGATGTTAACATCGGTCT+TGG + chr4.1:13043337-13043356 MS.gene27857:CDS 40.0%
GCTGTTCTTGGCATGAAATT+TGG - chr4.1:13043868-13043887 None:intergenic 40.0%
GGAAAATTTGCCTGCCAATT+TGG + chr4.1:13043591-13043610 MS.gene27857:CDS 40.0%
GGACGAAATCAAAGTTCTTG+AGG + chr4.1:13043822-13043841 MS.gene27857:CDS 40.0%
GTACTCTTGTGCAAATTCCT+TGG - chr4.1:13042853-13042872 None:intergenic 40.0%
TAGATGCCCTAGAAAACCTA+CGG + chr4.1:13042711-13042730 MS.gene27857:CDS 40.0%
TATACTCCTGCAAGTACTCA+GGG - chr4.1:13044140-13044159 None:intergenic 40.0%
TCACCAACAACAACAATGGT+GGG - chr4.1:13042770-13042789 None:intergenic 40.0%
TCTATTCAACATGTGCTGCA+AGG + chr4.1:13044078-13044097 MS.gene27857:CDS 40.0%
TGCAAACTATTATCAGCTGC+AGG + chr4.1:13044005-13044024 MS.gene27857:CDS 40.0%
TGTCTCTTCCCTGATATTGA+AGG - chr4.1:13044489-13044508 None:intergenic 40.0%
TTAACCATGGTTGATCTTCC+AGG + chr4.1:13043070-13043089 MS.gene27857:CDS 40.0%
TTATACTCCTGCAAGTACTC+AGG - chr4.1:13044141-13044160 None:intergenic 40.0%
TTCGTCCATTAGGAAATCCT+TGG - chr4.1:13043809-13043828 None:intergenic 40.0%
! ACCGCTGATGATGTTAACAT+CGG + chr4.1:13043331-13043350 MS.gene27857:CDS 40.0%
! CTGTTGATAAAACTGGTCTG+AGG + chr4.1:13043251-13043270 MS.gene27857:CDS 40.0%
! GCTGATAATAGTTTGCAGAG+TGG - chr4.1:13044002-13044021 None:intergenic 40.0%
! TTTTGCAGCCTCATAACCAA+TGG - chr4.1:13042875-13042894 None:intergenic 40.0%
!! CAAGAGTACCATTGGTTATG+AGG + chr4.1:13042864-13042883 MS.gene27857:CDS 40.0%
!! TCAGCATTTGTTGTTGACTC+AGG + chr4.1:13044278-13044297 MS.gene27857:CDS 40.0%
!!! TTTTCTAGGGCATCTAGAAC+AGG - chr4.1:13042707-13042726 None:intergenic 40.0%
AATGCCTGGAAGATCAACCA+TGG - chr4.1:13043077-13043096 None:intergenic 45.0%
ACAAGCTTCTGTGCCAAAAC+AGG - chr4.1:13043484-13043503 None:intergenic 45.0%
AGCTAATGACTCAAGGACAC+TGG - chr4.1:13042810-13042829 None:intergenic 45.0%
AGGCAAGGCTAATTGCCTAT+TGG + chr4.1:13044313-13044332 MS.gene27857:CDS 45.0%
AGTGTCCTTGAGTCATTAGC+TGG + chr4.1:13042809-13042828 MS.gene27857:CDS 45.0%
ATGTAACCCTGAGTACTTGC+AGG + chr4.1:13044131-13044150 MS.gene27857:CDS 45.0%
ATTACTCGTGTCCCTGTACA+TGG + chr4.1:13043094-13043113 MS.gene27857:CDS 45.0%
ATTGGAAGATTGTGCTGAGG+AGG + chr4.1:13044331-13044350 MS.gene27857:CDS 45.0%
CCTATTGGAAGATTGTGCTG+AGG + chr4.1:13044328-13044347 MS.gene27857:CDS 45.0%
CCTCAGCACAATCTTCCAAT+AGG - chr4.1:13044331-13044350 None:intergenic 45.0%
CTGTTATCAGCAAATGGTGG+TGG + chr4.1:13044438-13044457 MS.gene27857:CDS 45.0%
GAGGAGTCTCCTTCAATATC+AGG + chr4.1:13044477-13044496 MS.gene27857:CDS 45.0%
GCGTATCAACATTGCCAAAG+AGG + chr4.1:13042733-13042752 MS.gene27857:CDS 45.0%
TGCTATAAACACAGCCACTG+AGG + chr4.1:13042973-13042992 MS.gene27857:CDS 45.0%
! AAAGCTGACAAAGCACCAGA+AGG + chr4.1:13043292-13043311 MS.gene27857:CDS 45.0%
! CAACAACAATGGTGGGAAGT+TGG - chr4.1:13042763-13042782 None:intergenic 45.0%
! CTTCCCACCATTGTTGTTGT+TGG + chr4.1:13042764-13042783 MS.gene27857:CDS 45.0%
! GATACGCCGTAGGTTTTCTA+GGG - chr4.1:13042720-13042739 None:intergenic 45.0%
! TAGCCTTGCCTTCAAGTCAA+AGG - chr4.1:13044304-13044323 None:intergenic 45.0%
! TCTAAAACAAGCTCTGGCTG+TGG - chr4.1:13042908-13042927 None:intergenic 45.0%
! TGATACGCCGTAGGTTTTCT+AGG - chr4.1:13042721-13042740 None:intergenic 45.0%
!! GTTGTTGGTGATCAATCGTC+TGG + chr4.1:13042779-13042798 MS.gene27857:CDS 45.0%
!! TATCAGGTTGGCCATGTACA+GGG - chr4.1:13043108-13043127 None:intergenic 45.0%
!! TGTGCTGCAAGGTGTTGAAA+TGG + chr4.1:13044089-13044108 MS.gene27857:CDS 45.0%
!! TTATCAGGTTGGCCATGTAC+AGG - chr4.1:13043109-13043128 None:intergenic 45.0%
ATAAGTTTGCCACGTGGCCA+AGG + chr4.1:13042833-13042852 MS.gene27857:CDS 50.0%
GAGAGAAAGCAAAGAGACCG+TGG + chr4.1:13044536-13044555 MS.gene27857:CDS 50.0%
GCATTGCACCGCTAGATTAG+TGG + chr4.1:13043729-13043748 MS.gene27857:CDS 50.0%
GCCAATGATGCCAAATTGGC+AGG - chr4.1:13043604-13043623 None:intergenic 50.0%
GCCTGCCAATTTGGCATCAT+TGG + chr4.1:13043600-13043619 MS.gene27857:CDS 50.0%
GCTGGTATAAGTTTGCCACG+TGG + chr4.1:13042827-13042846 MS.gene27857:CDS 50.0%
GGAAGAGACAGAAGTTGAGC+AGG + chr4.1:13044499-13044518 MS.gene27857:CDS 50.0%
GGGAAGTTGGATTCCCTCTT+TGG - chr4.1:13042750-13042769 None:intergenic 50.0%
GGTGCAACAACAAGTGGTTG+AGG - chr4.1:13042653-13042672 None:intergenic 50.0%
TGGCAATGTTGATACGCCGT+AGG - chr4.1:13042730-13042749 None:intergenic 50.0%
! ACCTTTTCCAGCAAGCCTTC+TGG - chr4.1:13043310-13043329 None:intergenic 50.0%
!! ACCAGAAGGCTTGCTGGAAA+AGG + chr4.1:13043306-13043325 MS.gene27857:CDS 50.0%
!! AGGCCTTTGACTTGAAGGCA+AGG + chr4.1:13044298-13044317 MS.gene27857:CDS 50.0%
!! AGGGGTGTTGGAAATCCCTT+TGG - chr4.1:13043014-13043033 None:intergenic 50.0%
!! CAAATGGTGGTGGTGTTGAG+AGG + chr4.1:13044448-13044467 MS.gene27857:CDS 50.0%
!! GACTCAGGCCTTTGACTTGA+AGG + chr4.1:13044293-13044312 MS.gene27857:CDS 50.0%
ACAGGGACACGAGTAATGCC+TGG - chr4.1:13043091-13043110 None:intergenic 55.0%
GTGCAAATTCCTTGGCCACG+TGG - chr4.1:13042845-13042864 None:intergenic 55.0%
! ACAAGCTCTGGCTGTGGAAG+TGG - chr4.1:13042902-13042921 None:intergenic 55.0%
! CAAAGCACCAGAAGGCTTGC+TGG + chr4.1:13043300-13043319 MS.gene27857:CDS 55.0%
! TGGTGTTGAGAGGCTGCTTG+AGG + chr4.1:13044458-13044477 MS.gene27857:CDS 55.0%
!! AGCTTGCTGGCACTGCCAAA+GGG + chr4.1:13042996-13043015 MS.gene27857:CDS 55.0%
ACAGCCACTGAGGAGCTTGC+TGG + chr4.1:13042983-13043002 MS.gene27857:CDS 60.0%
AGTGCCAGCAAGCTCCTCAG+TGG - chr4.1:13042990-13043009 None:intergenic 60.0%
GCTCTGGCTGTGGAAGTGGA+TGG - chr4.1:13042898-13042917 None:intergenic 60.0%
!! GAGCTTGCTGGCACTGCCAA+AGG + chr4.1:13042995-13043014 MS.gene27857:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13042563 13044647 13042563 ID=MS.gene27857
chr4.1 mRNA 13042563 13044647 13042563 ID=MS.gene27857.t1;Parent=MS.gene27857
chr4.1 exon 13042563 13044647 13042563 ID=MS.gene27857.t1.exon1;Parent=MS.gene27857.t1
chr4.1 CDS 13042563 13044647 13042563 ID=cds.MS.gene27857.t1;Parent=MS.gene27857.t1
Gene Sequence

>MS.gene27857

ATGGCTTTTGGAAAGAAGAGTTCCACAAAATCTACTAAACGTGAAGAATCATCACTTGCATTGGTCCATGTTGATGTTGATGATCAACCTCAACCACTTGTTGTTGCACCAATAGTATCATCTTACAATGAGAAGATACGTCCTGTTCTAGATGCCCTAGAAAACCTACGGCGTATCAACATTGCCAAAGAGGGAATCCAACTTCCCACCATTGTTGTTGTTGGTGATCAATCGTCTGGCAAGTCCAGTGTCCTTGAGTCATTAGCTGGTATAAGTTTGCCACGTGGCCAAGGAATTTGCACAAGAGTACCATTGGTTATGAGGCTGCAAAACCATCCACTTCCACAGCCAGAGCTTGTTTTAGAGTATAATGGCAAGCAAGTTTCAACTGATGAGGAAAATGTATCTGATGCTATAAACACAGCCACTGAGGAGCTTGCTGGCACTGCCAAAGGGATTTCCAACACCCCTTTAACTTTGATTGTTAAGAAAAATGGTATTCCAGATTTAACCATGGTTGATCTTCCAGGCATTACTCGTGTCCCTGTACATGGCCAACCTGATAATATCTATGATCAAATTAAGGATATTATCATGGAGTACATAACACCAGAAGAAAGTATTATCTTGAATGTTCTTTCTGCCACGGTTGATTTTACTACTTGTGAGTCCATTAGGATGTCACAAACTGTTGATAAAACTGGTCTGAGGACACTTGCTGTCGTAACAAAAGCTGACAAAGCACCAGAAGGCTTGCTGGAAAAGGTAACCGCTGATGATGTTAACATCGGTCTTGGTTATGTTTGTGTAAGGAATAGAATTGGGGATGAGTCTTATGAAGATGCTAGAAATGAAGAAGAGAAGCTTTTTGAGGGACATTCACTTCTTTCCAAAATAGATAAATCTATTGTAGGAATTCCTGTTTTGGCACAGAAGCTTGTCCAAGTGCAAGCTATGATTATATCCAAAACTTTGCCTGAAATCATTAAGAAGATTAATGAGAAGTTGGCTAATAGTTTACATGAGTTGGAAAATTTGCCTGCCAATTTGGCATCATTGGCTGATGCCATGTCTGCTTTTCTGCAAATTATCTCTTTGTCAAGAGACTCTCTTAAAAAGATTCTTCTGATAGGGGAGTTTGAAGAGTATCCTGATGATAAGCAAATGCATTGCACCGCTAGATTAGTGGATATGTTGAATTCATATGCAACTGAACTTCAAAACTGTGCTGAAAGTAATATGACCAAGGATTTCCTAATGGACGAAATCAAAGTTCTTGAGGAAGCCAAGATTATTGGTCTTCCAAATTTCATGCCAAGAACAGCTTTTCTTACATTACTTCAGAGGAATGTAAGGGGCGTTTCTTATTTGCCAGTTAATTTTGTTGACAGTGTTTGGAACTATTTGGAAACTGTTGTTACTTCTGTTTTGAATCGCCACTCTGCAAACTATTATCAGCTGCAGGTTTCAATTCGTAGAGCTGCTGAGCATCTTATTGCCAAGAAGAAAAAGAATTCTATTCAACATGTGCTGCAAGGTGTTGAAATGGAGAAGCTTACAGACTACACATGTAACCCTGAGTACTTGCAGGAGTATAACAAGCTGATGTCTAATCAAGAAGCGTTTTTGAAAGAAGTGTTGAATGCAAATAGAGAATCAAGTACTGTGAATCTTGAAGGACATGGTGAGATTGAAGTTGCTCATCTGAAGAACTATCAGCATTTGTTGTTGACTCAGGCCTTTGACTTGAAGGCAAGGCTAATTGCCTATTGGAAGATTGTGCTGAGGAGGCTCATTGATGTTATTGCTTTGCATTTGATGTTAAGCATCAATGAGCTTGTGAATGTTGATCTGCAAAAGGAGATCTGTAATGAACTGTTATCAGCAAATGGTGGTGGTGTTGAGAGGCTGCTTGAGGAGTCTCCTTCAATATCAGGGAAGAGACAGAAGTTGAGCAGGAGTGTGACAGTTTTGAGAGAAAGCAAAGAGACCGTGGCCAACATTATGTTTGTTTTAGTTTGTTTTTATCTATCTTGCAAGTGTCCTTTTGTCATGTCTGTTTTGTTTGTCTGTTGGAACTTTTAG
Protein sequence

>MS.gene27857.t1

MAFGKKSSTKSTKREESSLALVHVDVDDQPQPLVVAPIVSSYNEKIRPVLDALENLRRINIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLVMRLQNHPLPQPELVLEYNGKQVSTDEENVSDAINTATEELAGTAKGISNTPLTLIVKKNGIPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFTTCESIRMSQTVDKTGLRTLAVVTKADKAPEGLLEKVTADDVNIGLGYVCVRNRIGDESYEDARNEEEKLFEGHSLLSKIDKSIVGIPVLAQKLVQVQAMIISKTLPEIIKKINEKLANSLHELENLPANLASLADAMSAFLQIISLSRDSLKKILLIGEFEEYPDDKQMHCTARLVDMLNSYATELQNCAESNMTKDFLMDEIKVLEEAKIIGLPNFMPRTAFLTLLQRNVRGVSYLPVNFVDSVWNYLETVVTSVLNRHSANYYQLQVSIRRAAEHLIAKKKKNSIQHVLQGVEMEKLTDYTCNPEYLQEYNKLMSNQEAFLKEVLNANRESSTVNLEGHGEIEVAHLKNYQHLLLTQAFDLKARLIAYWKIVLRRLIDVIALHLMLSINELVNVDLQKEICNELLSANGGGVERLLEESPSISGKRQKLSRSVTVLRESKETVANIMFVLVCFYLSCKCPFVMSVLFVCWNF