AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27858

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27858.t1 MTR_8g087750 88.791 678 15 2 1 617 1 678 0.0 1193
MS.gene27858.t1 MTR_8g087770 73.621 671 107 7 11 616 6 671 0.0 956
MS.gene27858.t1 MTR_8g087763 57.778 225 56 1 354 539 4 228 8.36e-81 259
MS.gene27858.t1 MTR_4g093750 39.716 282 148 8 69 333 35 311 2.34e-42 163
MS.gene27858.t1 MTR_4g093750 39.716 282 148 8 69 333 35 311 2.57e-42 163
MS.gene27858.t1 MTR_2g009690 36.027 297 172 7 65 344 40 335 1.77e-39 155
MS.gene27858.t1 MTR_5g009150 44.385 187 100 3 71 254 35 220 4.15e-34 137
MS.gene27858.t1 MTR_7g451010 40.094 212 117 5 71 278 40 245 8.82e-32 130
MS.gene27858.t1 MTR_8g105580 38.333 240 126 7 71 295 35 267 1.98e-31 129
MS.gene27858.t1 MTR_8g027675 38.389 211 123 4 71 276 34 242 2.04e-31 129
MS.gene27858.t1 MTR_8g105580 38.333 240 126 7 71 295 35 267 2.31e-31 129
MS.gene27858.t1 MTR_1g032550 40.107 187 108 3 71 254 35 220 3.12e-31 129
MS.gene27858.t1 MTR_8g099550 42.246 187 104 3 71 254 34 219 4.40e-31 129
MS.gene27858.t1 MTR_4g098920 41.063 207 106 6 71 270 34 231 1.57e-30 127
MS.gene27858.t1 MTR_8g009510 40.196 204 112 5 71 270 40 237 1.87e-29 124
MS.gene27858.t1 MTR_4g085860 72.368 76 20 1 208 282 2 77 3.03e-29 111
MS.gene27858.t1 MTR_6g045107 71.053 76 21 1 208 282 2 77 2.77e-27 105
MS.gene27858.t1 MTR_8g009510 36.567 134 79 2 138 270 2 130 5.62e-15 78.6
MS.gene27858.t1 MTR_8g463270 28.631 241 147 9 56 281 27 257 6.40e-15 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27858.t1 AT1G60500 54.134 641 229 6 35 612 29 667 0.0 684
MS.gene27858.t1 AT1G60530 70.934 289 78 1 22 310 19 301 3.00e-141 412
MS.gene27858.t1 AT4G33650 34.796 319 184 10 39 340 31 342 1.77e-38 152
MS.gene27858.t1 AT4G33650 34.796 319 184 10 39 340 31 342 1.81e-38 152
MS.gene27858.t1 AT2G14120 33.127 323 191 9 36 340 12 327 3.48e-36 145
MS.gene27858.t1 AT2G14120 33.127 323 191 9 36 340 12 327 3.64e-36 145
MS.gene27858.t1 AT2G14120 33.127 323 191 9 36 340 12 327 3.68e-36 145
MS.gene27858.t1 AT2G14120 33.127 323 191 9 36 340 12 327 4.33e-36 145
MS.gene27858.t1 AT1G14830 42.857 182 100 3 71 249 35 215 1.28e-32 133
MS.gene27858.t1 AT5G42080 39.810 211 121 4 71 276 34 243 1.48e-31 127
MS.gene27858.t1 AT2G44590 32.075 318 179 9 71 356 35 347 1.90e-31 130
MS.gene27858.t1 AT3G60190 39.810 211 121 4 71 276 40 249 3.08e-31 129
MS.gene27858.t1 AT5G42080 39.810 211 121 4 71 276 34 243 3.25e-31 129
MS.gene27858.t1 AT5G42080 39.810 211 121 4 71 276 34 243 3.35e-31 129
MS.gene27858.t1 AT5G42080 39.810 211 121 4 71 276 34 243 3.35e-31 129
MS.gene27858.t1 AT3G61760 41.667 204 109 5 71 270 34 231 5.10e-31 127
MS.gene27858.t1 AT3G61760 41.667 204 109 5 71 270 34 231 7.15e-31 128
MS.gene27858.t1 AT3G61760 41.667 204 109 5 71 270 34 231 1.07e-30 127
MS.gene27858.t1 AT2G44590 29.245 318 171 10 71 356 35 330 6.25e-22 100
MS.gene27858.t1 AT2G44590 29.245 318 171 10 71 356 35 330 6.62e-22 100
MS.gene27858.t1 AT1G53140 23.193 332 231 7 1 323 1 317 1.62e-14 77.4

Find 104 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGTCTCCGTCTATTTC+TGG 0.150723 4.1:+13008927 MS.gene27858:CDS
AGTCTCACTCACTTCTTTCT+AGG 0.252212 4.1:+13007876 MS.gene27858:CDS
ATTCTGAAGGCGCATAATTA+AGG 0.255203 4.1:-13007315 None:intergenic
TTACTACTTGTGAGTCTATC+AGG 0.258224 4.1:+13007660 MS.gene27858:CDS
TGGAAGCTGAATTCCTTCTT+TGG 0.309988 4.1:-13007189 None:intergenic
TTCAAATTATTATCAGCTTC+AGG 0.310159 4.1:+13008458 MS.gene27858:CDS
TTCTCAAGGGCGTCGAGAAC+TGG 0.318424 4.1:-13007146 None:intergenic
AGTATTTGCTCGAGTATAAT+AGG 0.323700 4.1:+13008595 MS.gene27858:CDS
CTTCCAACAATTGTTGTTGT+TGG 0.331397 4.1:+13007206 MS.gene27858:CDS
GGAGACTGTTGCAAGAATTA+TGG 0.333112 4.1:+13008998 MS.gene27858:CDS
GTTGTTGGAGATCAATCTTC+AGG 0.340523 4.1:+13007221 MS.gene27858:CDS
TCACAAATGGTTGATAAAAC+TGG 0.343252 4.1:+13007686 MS.gene27858:CDS
TTGAATTCTAACAAGAGTTC+TGG 0.352154 4.1:-13007353 None:intergenic
TTCAAGATAATACTTTCTTC+AGG 0.356152 4.1:-13007614 None:intergenic
CTTACCATGGTTGATCTTCC+AGG 0.356170 4.1:+13007512 MS.gene27858:CDS
TCAGATAAAAGATATTATTA+TGG 0.384953 4.1:+13007580 MS.gene27858:CDS
AGGCTAGGATGATTGCTTAC+TGG 0.386618 4.1:+13008763 MS.gene27858:CDS
AAGTTCAGTCCAACATGTTA+TGG 0.389392 4.1:+13008527 MS.gene27858:CDS
ACCTTCTCCAACAATCCTTC+AGG 0.403379 4.1:-13007749 None:intergenic
CAAAGTTCTTAGAGAAAGTA+AGG 0.404659 4.1:+13008977 MS.gene27858:CDS
CATAGGTCTCATCTCCAATT+CGG 0.409654 4.1:-13007820 None:intergenic
TCTCCAACAACAACAATTGT+TGG 0.419207 4.1:-13007209 None:intergenic
GTCTGTGTGCGAAACCGAAT+TGG 0.426739 4.1:+13007806 MS.gene27858:CDS
TCGACGCCCTTGAGAATCTA+AGG 0.430085 4.1:+13007153 MS.gene27858:CDS
ATCTGATCATAGATATTGTC+AGG 0.431759 4.1:-13007563 None:intergenic
CAGGTTTCCACTCGCAGAGC+AGG 0.433533 4.1:+13008477 MS.gene27858:CDS
CCAACACATGTGAAGCTTGA+AGG 0.436407 4.1:+13008672 MS.gene27858:CDS
CAATGCATGTGCTTGTCCTC+AGG 0.447247 4.1:-13008154 None:intergenic
AGGATTGACAAATCCATTGT+TGG 0.451509 4.1:+13007896 MS.gene27858:CDS
TAAATCTCCTGAAGGATTGT+TGG 0.453191 4.1:+13007742 MS.gene27858:CDS
GACCTATGAAGATGCTAGAT+TGG 0.455034 4.1:+13007835 MS.gene27858:CDS
ATCACCCGTGTACCTGTTCA+TGG 0.456607 4.1:+13007536 MS.gene27858:CDS
GAACTCTTGTTAGAATTCAA+TGG 0.458145 4.1:+13007356 MS.gene27858:CDS
TATGGAAGAGATTAAGGTGT+TGG 0.462977 4.1:+13008261 MS.gene27858:CDS
TTGGCATAGACCAATCCATT+TGG 0.463236 4.1:-13008290 None:intergenic
TGATACCAGCTAAAGATTCA+AGG 0.464998 4.1:-13007256 None:intergenic
TTCTGAAGGCGCATAATTAA+GGG 0.465234 4.1:-13007314 None:intergenic
AGTGTCCTTGAATCTTTAGC+TGG 0.467085 4.1:+13007251 MS.gene27858:CDS
AAGAGTTCTGGCTTTGAAAG+TGG 0.480074 4.1:-13007341 None:intergenic
CCTTCAAGCTTCACATGTGT+TGG 0.480389 4.1:-13008672 None:intergenic
CGTCTCAACATTGCCAAAGA+AGG 0.485759 4.1:+13007176 MS.gene27858:CDS
CTCTAAGAACTTTGACACTT+CGG 0.488083 4.1:-13008968 None:intergenic
CTCCGTCTATTTCTGGAAAG+AGG 0.488105 4.1:+13008934 MS.gene27858:CDS
ATAGCTACTGAAGAGATTGC+TGG 0.488901 4.1:+13007425 MS.gene27858:CDS
TCATTGTAGGATGAAACAAT+TGG 0.490493 4.1:-13007113 None:intergenic
TTGTCAGGTTGACCATGAAC+AGG 0.495557 4.1:-13007548 None:intergenic
AGGTGTTGGAAGAGGCCAAA+TGG 0.496352 4.1:+13008275 MS.gene27858:CDS
ACAGGTACACGGGTGATACC+TGG 0.496559 4.1:-13007530 None:intergenic
TTACCCTCATGTGCTAAGTC+AGG 0.501551 4.1:+13008728 MS.gene27858:CDS
GCAACAGAAGATAAATTAGC+AGG 0.505796 4.1:-13008043 None:intergenic
GCAAGAATTATGGACAGGAT+TGG 0.508761 4.1:+13009008 MS.gene27858:CDS
AGATCAACCATGGTAAGATC+AGG 0.510480 4.1:-13007506 None:intergenic
AATCAAAGTCAAAGGAGTGT+TGG 0.514073 4.1:-13007465 None:intergenic
TTCCAATCTAGCATCTTCAT+AGG 0.522397 4.1:-13007837 None:intergenic
GTCTATCAGGATGTCACAAA+TGG 0.523985 4.1:+13007673 MS.gene27858:CDS
GATGACGTTAACATCGGTCT+AGG 0.524437 4.1:+13007779 MS.gene27858:CDS
CTCTCAATCCCACCACCACT+TGG 0.524638 4.1:-13008897 None:intergenic
TTGGAAGAGGCCAAATGGAT+TGG 0.525795 4.1:+13008280 MS.gene27858:CDS
AGAGATTAAGGTGTTGGAAG+AGG 0.526369 4.1:+13008267 MS.gene27858:CDS
TTCTTGACAATCAAAGTCAA+AGG 0.538928 4.1:-13007473 None:intergenic
ATCAGCTTGCCACGTGGACA+AGG 0.540401 4.1:+13007275 MS.gene27858:CDS
CTGTTGCAAGAATTATGGAC+AGG 0.542640 4.1:+13009003 MS.gene27858:CDS
GAGGTTGAGCTTGCTCAACA+TGG 0.552600 4.1:-13007076 None:intergenic
AGGAGTGTTGGAAATCCCTC+TGG 0.553456 4.1:-13007453 None:intergenic
AATGCATGTGCTTGTCCTCA+GGG 0.555069 4.1:-13008153 None:intergenic
CAAGTGGTGGTGGGATTGAG+AGG 0.561054 4.1:+13008898 MS.gene27858:CDS
TGGTTGATAAAACTGGTTTG+AGG 0.562514 4.1:+13007693 MS.gene27858:CDS
ATTGGTGCAACAACAGAAAG+AGG 0.569115 4.1:-13007095 None:intergenic
TCCTGAAGGATTGTTGGAGA+AGG 0.571650 4.1:+13007748 MS.gene27858:CDS
AGCTAAAGATTCAAGGACAC+TGG 0.572468 4.1:-13007249 None:intergenic
TAAAATCAACAGTGGCGGAA+AGG 0.574096 4.1:-13007640 None:intergenic
TCCCTCTTTCCAGAAATAGA+CGG 0.574959 4.1:-13008936 None:intergenic
TCCGTCTATTTCTGGAAAGA+GGG 0.578371 4.1:+13008935 MS.gene27858:CDS
AAAGTGATGATTCATCTCGT+TGG 0.587870 4.1:-13007046 None:intergenic
ACTGGAAGATTGTTCTGAGG+AGG 0.593317 4.1:+13008781 MS.gene27858:CDS
CAACAGCTTCCATAACATGT+TGG 0.595030 4.1:-13008536 None:intergenic
TGTTATGGAAGCTGTTGAGA+TGG 0.595595 4.1:+13008542 MS.gene27858:CDS
GATTAACAATGATTTGCAGA+AGG 0.597025 4.1:+13008851 MS.gene27858:CDS
AAAGCCTGACTTAGCACATG+AGG 0.603423 4.1:-13008732 None:intergenic
CTTACTGGAAGATTGTTCTG+AGG 0.604360 4.1:+13008778 MS.gene27858:CDS
TTGAAAGTGGATGATTCTGA+AGG 0.605131 4.1:-13007328 None:intergenic
GAGATTGCTGGTTCTGCCAG+AGG 0.608773 4.1:+13007437 MS.gene27858:CDS
GTTGACCATGAACAGGTACA+CGG 0.609991 4.1:-13007541 None:intergenic
AGATTGCTGGTTCTGCCAGA+GGG 0.611506 4.1:+13007438 MS.gene27858:CDS
TTTCTCGCCTGCTCTGCGAG+TGG 0.614106 4.1:-13008484 None:intergenic
TGAGACGCCTTAGATTCTCA+AGG 0.620323 4.1:-13007160 None:intergenic
AAAGCAGATAAATCTCCTGA+AGG 0.623780 4.1:+13007734 MS.gene27858:CDS
GAGACGCCTTAGATTCTCAA+GGG 0.625868 4.1:-13007159 None:intergenic
TAAAACAGGAATACCAACAA+TGG 0.629558 4.1:-13007909 None:intergenic
TTATACTCGAGCAAATACTC+AGG 0.634135 4.1:-13008591 None:intergenic
TTGACCATGAACAGGTACAC+GGG 0.647300 4.1:-13007540 None:intergenic
AAATTGATGTTGTTAATCTG+AGG 0.650762 4.1:+13008703 MS.gene27858:CDS
AAGCCTGACTTAGCACATGA+GGG 0.658164 4.1:-13008731 None:intergenic
AGTAGTAAAATCAACAGTGG+CGG 0.662087 4.1:-13007645 None:intergenic
GTGCAGATTCCTTGTCCACG+TGG 0.668352 4.1:-13007284 None:intergenic
CATGTGAAGCTTGAAGGTGT+TGG 0.674753 4.1:+13008678 MS.gene27858:CDS
TGGTGTTCCTGATCTTACCA+TGG 0.685138 4.1:+13007499 MS.gene27858:CDS
ACTGCTGATGACGTTAACAT+CGG 0.693473 4.1:+13007773 MS.gene27858:CDS
GCTGGTATCAGCTTGCCACG+TGG 0.693476 4.1:+13007269 MS.gene27858:CDS
GTGGACAAGGAATCTGCACG+AGG 0.706834 4.1:+13007288 MS.gene27858:CDS
ACAAGTAGTAAAATCAACAG+TGG 0.707970 4.1:-13007648 None:intergenic
GATACCTGGAAGATCAACCA+TGG 0.712735 4.1:-13007516 None:intergenic
TGGACAAGGAATCTGCACGA+GGG 0.740882 4.1:+13007289 MS.gene27858:CDS
TGATTTCGAAGAGTACCCTG+AGG 0.804578 4.1:+13008138 MS.gene27858:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAGATAAAAGATATTATTA+TGG + chr4.1:13007580-13007599 MS.gene27858:CDS 15.0%
! AAATTGATGTTGTTAATCTG+AGG + chr4.1:13008703-13008722 MS.gene27858:CDS 25.0%
! ACTTTGATTGTCAAGAAAAA+TGG + chr4.1:13007479-13007498 MS.gene27858:CDS 25.0%
! TTCAAATTATTATCAGCTTC+AGG + chr4.1:13008458-13008477 MS.gene27858:CDS 25.0%
! TTCAAGATAATACTTTCTTC+AGG - chr4.1:13007617-13007636 None:intergenic 25.0%
!! TGCGAAAAAGAATTTTCTTA+TGG + chr4.1:13008243-13008262 MS.gene27858:CDS 25.0%
!! TTTTCTTATGGAAGAGATTA+AGG + chr4.1:13008255-13008274 MS.gene27858:CDS 25.0%
!!! CTAACTTTGTTGATAATGTT+TGG + chr4.1:13008391-13008410 MS.gene27858:intron 25.0%
AATACTGCAGAGAAAAGTTA+AGG + chr4.1:13008349-13008368 MS.gene27858:intron 30.0%
ACAAGTAGTAAAATCAACAG+TGG - chr4.1:13007651-13007670 None:intergenic 30.0%
AGTATTTGCTCGAGTATAAT+AGG + chr4.1:13008595-13008614 MS.gene27858:CDS 30.0%
ATACTGCAGAGAAAAGTTAA+GGG + chr4.1:13008350-13008369 MS.gene27858:intron 30.0%
ATCTGATCATAGATATTGTC+AGG - chr4.1:13007566-13007585 None:intergenic 30.0%
ATTGTCAAAAATGCAGTTCT+TGG - chr4.1:13008312-13008331 None:intergenic 30.0%
CAAAGTTCTTAGAGAAAGTA+AGG + chr4.1:13008977-13008996 MS.gene27858:CDS 30.0%
CTCAAAAAGATTCTTCTTAC+AGG + chr4.1:13008115-13008134 MS.gene27858:CDS 30.0%
GAACTCTTGTTAGAATTCAA+TGG + chr4.1:13007356-13007375 MS.gene27858:CDS 30.0%
TAAAACAGGAATACCAACAA+TGG - chr4.1:13007912-13007931 None:intergenic 30.0%
TCATTGTAGGATGAAACAAT+TGG - chr4.1:13007116-13007135 None:intergenic 30.0%
TTCTTGACAATCAAAGTCAA+AGG - chr4.1:13007476-13007495 None:intergenic 30.0%
TTGAATTCTAACAAGAGTTC+TGG - chr4.1:13007356-13007375 None:intergenic 30.0%
! ACAGCTTTTATGCATATTCT+TGG + chr4.1:13008076-13008095 MS.gene27858:CDS 30.0%
! GATTAACAATGATTTGCAGA+AGG + chr4.1:13008851-13008870 MS.gene27858:CDS 30.0%
! TTAATCTTCTTGATGATCTC+TGG - chr4.1:13007983-13008002 None:intergenic 30.0%
!! TCACAAATGGTTGATAAAAC+TGG + chr4.1:13007686-13007705 MS.gene27858:CDS 30.0%
!!! TGGACGTATTTTTTCATTGT+AGG - chr4.1:13007129-13007148 None:intergenic 30.0%
AAAGCAGATAAATCTCCTGA+AGG + chr4.1:13007734-13007753 MS.gene27858:CDS 35.0%
AAAGTGATGATTCATCTCGT+TGG - chr4.1:13007049-13007068 None:intergenic 35.0%
AAGTTAAGGGCAACATGTTA+TGG + chr4.1:13008363-13008382 MS.gene27858:intron 35.0%
AAGTTCAGTCCAACATGTTA+TGG + chr4.1:13008527-13008546 MS.gene27858:CDS 35.0%
AGGATTGACAAATCCATTGT+TGG + chr4.1:13007896-13007915 MS.gene27858:CDS 35.0%
AGTAGTAAAATCAACAGTGG+CGG - chr4.1:13007648-13007667 None:intergenic 35.0%
GCAACAGAAGATAAATTAGC+AGG - chr4.1:13008046-13008065 None:intergenic 35.0%
TAAATCTCCTGAAGGATTGT+TGG + chr4.1:13007742-13007761 MS.gene27858:CDS 35.0%
TATGGAAGAGATTAAGGTGT+TGG + chr4.1:13008261-13008280 MS.gene27858:CDS 35.0%
TCTCCAACAACAACAATTGT+TGG - chr4.1:13007212-13007231 None:intergenic 35.0%
TGATACCAGCTAAAGATTCA+AGG - chr4.1:13007259-13007278 None:intergenic 35.0%
TTACTACTTGTGAGTCTATC+AGG + chr4.1:13007660-13007679 MS.gene27858:CDS 35.0%
TTATACTCGAGCAAATACTC+AGG - chr4.1:13008594-13008613 None:intergenic 35.0%
TTCCAATCTAGCATCTTCAT+AGG - chr4.1:13007840-13007859 None:intergenic 35.0%
TTGAAAGTGGATGATTCTGA+AGG - chr4.1:13007331-13007350 None:intergenic 35.0%
! AATCAAAGTCAAAGGAGTGT+TGG - chr4.1:13007468-13007487 None:intergenic 35.0%
! AATGATCTTTTGTCTCCAAG+TGG + chr4.1:13008882-13008901 MS.gene27858:CDS 35.0%
! ACTAACACAAGCTTTTGTAG+TGG - chr4.1:13007021-13007040 None:intergenic 35.0%
! ATTCTGAAGGCGCATAATTA+AGG - chr4.1:13007318-13007337 None:intergenic 35.0%
! CTCTAAGAACTTTGACACTT+CGG - chr4.1:13008971-13008990 None:intergenic 35.0%
! CTTCCAACAATTGTTGTTGT+TGG + chr4.1:13007206-13007225 MS.gene27858:CDS 35.0%
! TTCTGAAGGCGCATAATTAA+GGG - chr4.1:13007317-13007336 None:intergenic 35.0%
! TTTAGCTCAGAAGCTTGTTT+AGG + chr4.1:13007928-13007947 MS.gene27858:intron 35.0%
!! TGGTTGATAAAACTGGTTTG+AGG + chr4.1:13007693-13007712 MS.gene27858:CDS 35.0%
!!! AAGTCAGGCTTTTGATTTGA+AGG + chr4.1:13008743-13008762 MS.gene27858:CDS 35.0%
ACAAGCTTCTGAGCTAAAAC+AGG - chr4.1:13007926-13007945 None:intergenic 40.0%
AGATCAACCATGGTAAGATC+AGG - chr4.1:13007509-13007528 None:intergenic 40.0%
AGCTAAAGATTCAAGGACAC+TGG - chr4.1:13007252-13007271 None:intergenic 40.0%
AGTCTCACTCACTTCTTTCT+AGG + chr4.1:13007876-13007895 MS.gene27858:CDS 40.0%
AGTGTCCTTGAATCTTTAGC+TGG + chr4.1:13007251-13007270 MS.gene27858:CDS 40.0%
ATAGCTACTGAAGAGATTGC+TGG + chr4.1:13007425-13007444 MS.gene27858:CDS 40.0%
ATTGGTGCAACAACAGAAAG+AGG - chr4.1:13007098-13007117 None:intergenic 40.0%
CAACAGCTTCCATAACATGT+TGG - chr4.1:13008539-13008558 None:intergenic 40.0%
CATAGGTCTCATCTCCAATT+CGG - chr4.1:13007823-13007842 None:intergenic 40.0%
CTGTTGCAAGAATTATGGAC+AGG + chr4.1:13009003-13009022 MS.gene27858:CDS 40.0%
CTTACTGGAAGATTGTTCTG+AGG + chr4.1:13008778-13008797 MS.gene27858:CDS 40.0%
GACCTATGAAGATGCTAGAT+TGG + chr4.1:13007835-13007854 MS.gene27858:CDS 40.0%
GCAAGAATTATGGACAGGAT+TGG + chr4.1:13009008-13009027 MS.gene27858:CDS 40.0%
GGAGACTGTTGCAAGAATTA+TGG + chr4.1:13008998-13009017 MS.gene27858:CDS 40.0%
GTCTATCAGGATGTCACAAA+TGG + chr4.1:13007673-13007692 MS.gene27858:CDS 40.0%
GTTGTTGGAGATCAATCTTC+AGG + chr4.1:13007221-13007240 MS.gene27858:CDS 40.0%
TCCCTCTTTCCAGAAATAGA+CGG - chr4.1:13008939-13008958 None:intergenic 40.0%
TGGAAGCTGAATTCCTTCTT+TGG - chr4.1:13007192-13007211 None:intergenic 40.0%
TGTTATGGAAGCTGTTGAGA+TGG + chr4.1:13008542-13008561 MS.gene27858:CDS 40.0%
TTGGCATAGACCAATCCATT+TGG - chr4.1:13008293-13008312 None:intergenic 40.0%
! ACTGCTGATGACGTTAACAT+CGG + chr4.1:13007773-13007792 MS.gene27858:CDS 40.0%
! TAAAATCAACAGTGGCGGAA+AGG - chr4.1:13007643-13007662 None:intergenic 40.0%
!! AAGAGTTCTGGCTTTGAAAG+TGG - chr4.1:13007344-13007363 None:intergenic 40.0%
!! AGAGATTAAGGTGTTGGAAG+AGG + chr4.1:13008267-13008286 MS.gene27858:CDS 40.0%
!! TCCGTCTATTTCTGGAAAGA+GGG + chr4.1:13008935-13008954 MS.gene27858:CDS 40.0%
!!! AGGCTTTTGATTTGAAGGCT+AGG + chr4.1:13008748-13008767 MS.gene27858:CDS 40.0%
AAAGCCTGACTTAGCACATG+AGG - chr4.1:13008735-13008754 None:intergenic 45.0%
AAGCCTGACTTAGCACATGA+GGG - chr4.1:13008734-13008753 None:intergenic 45.0%
AATGCATGTGCTTGTCCTCA+GGG - chr4.1:13008156-13008175 None:intergenic 45.0%
ACCTTCTCCAACAATCCTTC+AGG - chr4.1:13007752-13007771 None:intergenic 45.0%
AGGCTAGGATGATTGCTTAC+TGG + chr4.1:13008763-13008782 MS.gene27858:CDS 45.0%
CCAACACATGTGAAGCTTGA+AGG + chr4.1:13008672-13008691 MS.gene27858:CDS 45.0%
CCTTCAAGCTTCACATGTGT+TGG - chr4.1:13008675-13008694 None:intergenic 45.0%
CGTCTCAACATTGCCAAAGA+AGG + chr4.1:13007176-13007195 MS.gene27858:CDS 45.0%
CTTACCATGGTTGATCTTCC+AGG + chr4.1:13007512-13007531 MS.gene27858:CDS 45.0%
GAAGAGTCTCCGTCTATTTC+TGG + chr4.1:13008927-13008946 MS.gene27858:CDS 45.0%
GAGACGCCTTAGATTCTCAA+GGG - chr4.1:13007162-13007181 None:intergenic 45.0%
GATACCTGGAAGATCAACCA+TGG - chr4.1:13007519-13007538 None:intergenic 45.0%
GATGACGTTAACATCGGTCT+AGG + chr4.1:13007779-13007798 MS.gene27858:CDS 45.0%
GTTGACCATGAACAGGTACA+CGG - chr4.1:13007544-13007563 None:intergenic 45.0%
TCCTGAAGGATTGTTGGAGA+AGG + chr4.1:13007748-13007767 MS.gene27858:CDS 45.0%
TGAGACGCCTTAGATTCTCA+AGG - chr4.1:13007163-13007182 None:intergenic 45.0%
TGGTGTTCCTGATCTTACCA+TGG + chr4.1:13007499-13007518 MS.gene27858:CDS 45.0%
TTACCCTCATGTGCTAAGTC+AGG + chr4.1:13008728-13008747 MS.gene27858:CDS 45.0%
TTGACCATGAACAGGTACAC+GGG - chr4.1:13007543-13007562 None:intergenic 45.0%
TTGGAAGAGGCCAAATGGAT+TGG + chr4.1:13008280-13008299 MS.gene27858:CDS 45.0%
! CATGTGAAGCTTGAAGGTGT+TGG + chr4.1:13008678-13008697 MS.gene27858:CDS 45.0%
! GATCTTTTGTCTCCAAGTGG+TGG + chr4.1:13008885-13008904 MS.gene27858:CDS 45.0%
! TTTTGTCTCCAAGTGGTGGT+GGG + chr4.1:13008889-13008908 MS.gene27858:CDS 45.0%
!! ACTGGAAGATTGTTCTGAGG+AGG + chr4.1:13008781-13008800 MS.gene27858:CDS 45.0%
!! CTCCGTCTATTTCTGGAAAG+AGG + chr4.1:13008934-13008953 MS.gene27858:CDS 45.0%
!! TGATTTCGAAGAGTACCCTG+AGG + chr4.1:13008138-13008157 MS.gene27858:CDS 45.0%
!! TTGTCAGGTTGACCATGAAC+AGG - chr4.1:13007551-13007570 None:intergenic 45.0%
ATCACCCGTGTACCTGTTCA+TGG + chr4.1:13007536-13007555 MS.gene27858:CDS 50.0%
CAATGCATGTGCTTGTCCTC+AGG - chr4.1:13008157-13008176 None:intergenic 50.0%
GTCTGTGTGCGAAACCGAAT+TGG + chr4.1:13007806-13007825 MS.gene27858:CDS 50.0%
TCGACGCCCTTGAGAATCTA+AGG + chr4.1:13007153-13007172 MS.gene27858:CDS 50.0%
TGGACAAGGAATCTGCACGA+GGG + chr4.1:13007289-13007308 MS.gene27858:CDS 50.0%
! CTTTTGTCTCCAAGTGGTGG+TGG + chr4.1:13008888-13008907 MS.gene27858:CDS 50.0%
! GAGGTTGAGCTTGCTCAACA+TGG - chr4.1:13007079-13007098 None:intergenic 50.0%
!! AGATTGCTGGTTCTGCCAGA+GGG + chr4.1:13007438-13007457 MS.gene27858:CDS 50.0%
!! AGGAGTGTTGGAAATCCCTC+TGG - chr4.1:13007456-13007475 None:intergenic 50.0%
!! AGGTGTTGGAAGAGGCCAAA+TGG + chr4.1:13008275-13008294 MS.gene27858:CDS 50.0%
ACAGGTACACGGGTGATACC+TGG - chr4.1:13007533-13007552 None:intergenic 55.0%
ATCAGCTTGCCACGTGGACA+AGG + chr4.1:13007275-13007294 MS.gene27858:CDS 55.0%
CTCTCAATCCCACCACCACT+TGG - chr4.1:13008900-13008919 None:intergenic 55.0%
GTGCAGATTCCTTGTCCACG+TGG - chr4.1:13007287-13007306 None:intergenic 55.0%
GTGGACAAGGAATCTGCACG+AGG + chr4.1:13007288-13007307 MS.gene27858:CDS 55.0%
!! CAAGTGGTGGTGGGATTGAG+AGG + chr4.1:13008898-13008917 MS.gene27858:CDS 55.0%
!! GAGATTGCTGGTTCTGCCAG+AGG + chr4.1:13007437-13007456 MS.gene27858:CDS 55.0%
!! TTCTCAAGGGCGTCGAGAAC+TGG - chr4.1:13007149-13007168 None:intergenic 55.0%
CAGGTTTCCACTCGCAGAGC+AGG + chr4.1:13008477-13008496 MS.gene27858:CDS 60.0%
GCTGGTATCAGCTTGCCACG+TGG + chr4.1:13007269-13007288 MS.gene27858:CDS 60.0%
TTTCTCGCCTGCTCTGCGAG+TGG - chr4.1:13008487-13008506 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13006996 13009043 13006996 ID=MS.gene27858
chr4.1 mRNA 13006996 13009043 13006996 ID=MS.gene27858.t1;Parent=MS.gene27858
chr4.1 exon 13006996 13007917 13006996 ID=MS.gene27858.t1.exon1;Parent=MS.gene27858.t1
chr4.1 CDS 13006996 13007917 13006996 ID=cds.MS.gene27858.t1;Parent=MS.gene27858.t1
chr4.1 exon 13007984 13008301 13007984 ID=MS.gene27858.t1.exon2;Parent=MS.gene27858.t1
chr4.1 CDS 13007984 13008301 13007984 ID=cds.MS.gene27858.t1;Parent=MS.gene27858.t1
chr4.1 exon 13008430 13009043 13008430 ID=MS.gene27858.t1.exon3;Parent=MS.gene27858.t1
chr4.1 CDS 13008430 13009043 13008430 ID=cds.MS.gene27858.t1;Parent=MS.gene27858.t1
Gene Sequence

>MS.gene27858

ATGGTTGTGACTTCAAAGAGAACCACTACAAAAGCTTGTGTTAGTGCTAACCAACGAGATGAATCATCACTTTCATTAGTCCATGTTGAGCAAGCTCAACCTCTTTCTGTTGTTGCACCAATTGTTTCATCCTACAATGAAAAAATACGTCCAGTTCTCGACGCCCTTGAGAATCTAAGGCGTCTCAACATTGCCAAAGAAGGAATTCAGCTTCCAACAATTGTTGTTGTTGGAGATCAATCTTCAGGAAAGTCCAGTGTCCTTGAATCTTTAGCTGGTATCAGCTTGCCACGTGGACAAGGAATCTGCACGAGGGTACCCTTAATTATGCGCCTTCAGAATCATCCACTTTCAAAGCCAGAACTCTTGTTAGAATTCAATGGCAAGACAGTTTTCACTGATGAAAAAGATGTTTCTGATGCAATAAACATAGCTACTGAAGAGATTGCTGGTTCTGCCAGAGGGATTTCCAACACTCCTTTGACTTTGATTGTCAAGAAAAATGGTGTTCCTGATCTTACCATGGTTGATCTTCCAGGTATCACCCGTGTACCTGTTCATGGTCAACCTGACAATATCTATGATCAGATAAAAGATATTATTATGGAGTATATAACTCCTGAAGAAAGTATTATCTTGAATGTCCTTTCCGCCACTGTTGATTTTACTACTTGTGAGTCTATCAGGATGTCACAAATGGTTGATAAAACTGGTTTGAGGACACTAGCTGTTGTGACAAAAGCAGATAAATCTCCTGAAGGATTGTTGGAGAAGGTTACTGCTGATGACGTTAACATCGGTCTAGGTTATGTCTGTGTGCGAAACCGAATTGGAGATGAGACCTATGAAGATGCTAGATTGGAAGAAGAAAAGCTGTTCGAGTCTCACTCACTTCTTTCTAGGATTGACAAATCCATTGTTGGTATTCCTGTTTTAGCTCAGAAGCTTGTTTAGGTTCAAGCTATGATTATCTCAAAAACTTTGCCAGAGATCATCAAGAAGATTAATGAGAAGCTTGCTTCTAATGCAAATGAGTTAGAGAATTTACCTGCTAATTTATCTTCTGTTGCTGATGCTATGACAGCTTTTATGCATATTCTTGGTTTGTCTAGAGATTCTCTCAAAAAGATTCTTCTTACAGGTGATTTCGAAGAGTACCCTGAGGACAAGCACATGCATTGCACTGCTCGATTAGTTGAGATGCTTAACTTATATGCAAGCGATCTTGAAAACTGTGCTGAAAGTGATGCGAAAAAGAATTTTCTTATGGAAGAGATTAAGGTGTTGGAAGAGGCCAAATGGATTGGTCTATGCCAAGAACTGCATTTTTGACAATACTACTGCATTTTTGACAATACTGCAGAGAAAAGTTAAGGGCAACATGTTATGGAAGTTCTAACTTTGTTGATAATGTTTGGAACTATCTTGAATCAGTTGTGATTTCTATTTTGAATCATCATTCTTCAAATTATTATCAGCTTCAGGTTTCCACTCGCAGAGCAGGCGAGAAATTGATTGCTAAGAAGAAGAAAAGTTCAGTCCAACATGTTATGGAAGCTGTTGAGATGGAAAAACTGACAGATTACACTTGTAATCCTGAGTATTTGCTCGAGTATAATAGGCTGATAGCTCAACAAGAAGCGTTTGTGAAAGAAGTTCTAAGCAATGAAACAAAACCAACACATGTGAAGCTTGAAGGTGTTGGTGAAATTGATGTTGTTAATCTGAGGAATTACCCTCATGTGCTAAGTCAGGCTTTTGATTTGAAGGCTAGGATGATTGCTTACTGGAAGATTGTTCTGAGGAGGCTCATTGACAGCATTGCTCTGCACTTGATGCTAAGCATCAATGAACTGATTAACAATGATTTGCAGAAGGAGATTTGTAATGATCTTTTGTCTCCAAGTGGTGGTGGGATTGAGAGGCTGCTTGAAGAGTCTCCGTCTATTTCTGGAAAGAGGGAGAAGCTAAGCCGAAGTGTCAAAGTTCTTAGAGAAAGTAAGGAGACTGTTGCAAGAATTATGGACAGGATTGGAATCTATGATTAG
Protein sequence

>MS.gene27858.t1

MVVTSKRTTTKACVSANQRDESSLSLVHVEQAQPLSVVAPIVSSYNEKIRPVLDALENLRRLNIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLIMRLQNHPLSKPELLLEFNGKTVFTDEKDVSDAINIATEEIAGSARGISNTPLTLIVKKNGVPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFTTCESIRMSQMVDKTGLRTLAVVTKADKSPEGLLEKVTADDVNIGLGYVCVRNRIGDETYEDARLEEEKLFESHSLLSRIDKSIVEIIKKINEKLASNANELENLPANLSSVADAMTAFMHILGLSRDSLKKILLTGDFEEYPEDKHMHCTARLVEMLNLYASDLENCAESDAKKNFLMEEIKVLEEAKWIVVISILNHHSSNYYQLQVSTRRAGEKLIAKKKKSSVQHVMEAVEMEKLTDYTCNPEYLLEYNRLIAQQEAFVKEVLSNETKPTHVKLEGVGEIDVVNLRNYPHVLSQAFDLKARMIAYWKIVLRRLIDSIALHLMLSINELINNDLQKEICNDLLSPSGGGIERLLEESPSISGKREKLSRSVKVLRESKETVARIMDRIGIYD