AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27953


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27953.t1 MTR_8g089110 99.174 121 1 0 1 121 1 121 1.21e-87 250
MS.gene27953.t1 MTR_8g088830 77.869 122 26 1 1 121 1 122 1.37e-56 172
MS.gene27953.t1 MTR_3g089035 60.800 125 44 1 1 120 1 125 1.93e-51 159
MS.gene27953.t1 MTR_3g089005 59.843 127 44 2 1 121 1 126 1.09e-50 157
MS.gene27953.t1 MTR_2g043730 54.839 124 52 1 1 120 1 124 2.94e-45 143
MS.gene27953.t1 MTR_2g043790 52.000 100 48 0 22 121 1 100 1.26e-31 108
MS.gene27953.t1 MTR_2g088990 40.870 115 63 4 9 119 6 119 1.26e-24 94.0
MS.gene27953.t1 MTR_7g086160 39.837 123 69 4 3 120 1 123 1.73e-22 86.7
MS.gene27953.t1 MTR_7g086140 36.885 122 73 3 3 120 1 122 1.38e-21 84.3
MS.gene27953.t1 MTR_7g086190 36.885 122 73 3 3 120 1 122 1.38e-21 84.3
MS.gene27953.t1 MTR_1g105130 40.171 117 66 3 7 119 4 120 2.10e-21 83.6
MS.gene27953.t1 MTR_1g105120 40.171 117 66 3 7 119 4 120 2.10e-21 83.6
MS.gene27953.t1 MTR_7g086220 39.344 122 67 4 1 118 1 119 5.20e-21 82.8
MS.gene27953.t1 MTR_1g104800 34.483 116 67 3 11 119 8 121 1.84e-20 82.0
MS.gene27953.t1 MTR_7g086230 39.344 122 69 3 1 119 1 120 6.09e-20 80.5
MS.gene27953.t1 MTR_7g086280 39.344 122 69 3 1 119 1 120 6.23e-20 80.5
MS.gene27953.t1 MTR_8g007020 41.379 116 60 5 11 119 5 119 1.37e-19 79.7
MS.gene27953.t1 MTR_5g006040 33.871 124 75 3 1 119 1 122 1.39e-19 79.7
MS.gene27953.t1 MTR_7g086100 38.525 122 70 3 1 119 1 120 2.07e-19 80.1
MS.gene27953.t1 MTR_1g112700 35.897 117 69 4 8 120 15 129 2.63e-18 76.3
MS.gene27953.t1 MTR_6g083240 38.211 123 65 3 5 118 6 126 3.53e-18 75.9
MS.gene27953.t1 MTR_8g007035 38.793 116 63 5 11 119 5 119 6.60e-18 75.9
MS.gene27953.t1 MTR_4g114870 40.000 100 53 2 27 119 190 289 1.21e-17 77.4
MS.gene27953.t1 MTR_4g114870 35.537 121 67 3 10 119 7 127 2.90e-15 70.5
MS.gene27953.t1 MTR_6g080660 36.170 94 56 3 28 118 28 120 3.85e-17 73.9
MS.gene27953.t1 MTR_0334s0010 33.065 124 76 3 1 118 1 123 7.22e-17 73.2
MS.gene27953.t1 MTR_6g023760 37.000 100 58 4 25 120 23 121 5.62e-16 70.1
MS.gene27953.t1 MTR_3g099980 38.596 114 64 5 11 118 7 120 1.72e-15 69.7
MS.gene27953.t1 MTR_7g086090 37.705 122 71 3 1 119 1 120 2.72e-15 69.7
MS.gene27953.t1 MTR_3g099570 38.793 116 61 6 11 119 7 119 4.25e-15 68.2
MS.gene27953.t1 MTR_3g099680 37.391 115 66 5 11 119 7 121 2.07e-14 67.0
MS.gene27953.t1 MTR_6g013200 30.952 126 79 3 1 120 8 131 3.62e-14 65.5
MS.gene27953.t1 MTR_8g086360 30.769 117 76 2 7 118 3 119 9.00e-14 64.7
MS.gene27953.t1 MTR_2g079030 33.333 114 68 3 13 118 7 120 3.80e-13 64.7
MS.gene27953.t1 MTR_3g099540 36.752 117 68 5 7 118 4 119 5.16e-13 61.6
MS.gene27953.t1 MTR_6g013420 33.613 119 74 3 7 120 16 134 5.81e-13 62.4
MS.gene27953.t1 MTR_8g099220 32.759 116 64 4 13 120 18 127 9.71e-13 60.8
MS.gene27953.t1 MTR_4g067200 25.385 130 88 2 1 121 1 130 1.32e-12 61.2
MS.gene27953.t1 MTR_4g066110 25.385 130 88 2 1 121 1 130 1.32e-12 61.2
MS.gene27953.t1 MTR_1g014120 31.897 116 75 2 7 118 8 123 2.98e-12 60.5
MS.gene27953.t1 MTR_8g463180 31.304 115 74 2 9 118 5 119 4.90e-12 61.2
MS.gene27953.t1 MTR_4g114830 33.019 106 59 4 21 115 21 125 1.52e-11 58.2
MS.gene27953.t1 MTR_4g007250 31.250 96 64 1 25 118 26 121 1.78e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27953.t1 AT2G02850 58.594 128 45 1 1 120 1 128 1.12e-50 157
MS.gene27953.t1 AT3G17675 36.066 122 73 3 5 121 7 128 8.82e-22 84.0
MS.gene27953.t1 AT2G32300 37.391 115 66 3 11 119 6 120 2.70e-20 83.2
MS.gene27953.t1 AT2G32300 37.391 115 66 3 11 119 36 150 4.45e-20 83.2
MS.gene27953.t1 AT1G17800 39.496 119 66 4 8 120 18 136 5.74e-20 79.7
MS.gene27953.t1 AT3G60270 42.857 98 52 3 25 119 23 119 6.06e-20 80.9
MS.gene27953.t1 AT5G26330 37.168 113 65 3 12 119 9 120 1.88e-19 79.3
MS.gene27953.t1 AT3G27200 36.134 119 71 4 7 121 5 122 4.35e-19 78.2
MS.gene27953.t1 AT3G60280 44.898 98 48 4 25 118 21 116 5.93e-18 76.3
MS.gene27953.t1 AT2G31050 33.858 127 73 4 1 119 1 124 3.76e-17 73.9
MS.gene27953.t1 AT2G26720 32.743 113 72 2 12 120 13 125 7.33e-17 73.2
MS.gene27953.t1 AT2G44790 43.137 102 48 5 23 119 27 123 1.37e-16 72.4
MS.gene27953.t1 AT5G07475 34.821 112 69 3 11 119 13 123 5.99e-16 70.5
MS.gene27953.t1 AT2G25060 36.190 105 59 3 22 118 25 129 1.48e-13 63.9
MS.gene27953.t1 AT3G20570 31.858 113 70 3 12 118 15 126 4.10e-13 63.2
MS.gene27953.t1 AT1G48940 33.077 130 65 8 6 120 3 125 1.76e-12 61.2
MS.gene27953.t1 AT4G12880 30.081 123 79 4 3 118 1 123 3.87e-12 59.3
MS.gene27953.t1 AT4G27520 33.913 115 67 3 9 118 16 126 1.02e-11 60.5
MS.gene27953.t1 AT3G18590 31.148 122 69 7 9 120 10 126 1.09e-11 59.3
MS.gene27953.t1 AT2G27035 30.508 118 76 3 8 120 8 124 1.14e-11 58.5
MS.gene27953.t1 AT1G22480 35.965 114 67 4 11 121 4 114 1.43e-11 58.5
MS.gene27953.t1 AT4G31840 28.814 118 76 3 9 118 8 125 1.56e-11 58.5
MS.gene27953.t1 AT1G72230 33.628 113 71 3 9 118 4 115 1.94e-11 58.5
MS.gene27953.t1 AT3G53330 32.039 103 64 3 22 118 182 284 4.31e-11 58.9

Find 38 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCTAATGGAAAACGCTTT+AGG 0.303848 4.4:+12031422 MS.gene27953:CDS
ACCTACACTGTTGGAGGTCC+TGG 0.340570 4.4:+12031373 MS.gene27953:CDS
AGGCCACCCAACAGTGTTAA+AGG 0.340614 4.4:-12031404 None:intergenic
GCCTAATGGAAAACGCTTTA+GGG 0.345587 4.4:+12031423 MS.gene27953:CDS
AACACTGTTGGGTGGCCTAA+TGG 0.351296 4.4:+12031409 MS.gene27953:CDS
TAAAAGTTAAGCAGCATTGA+TGG 0.375502 4.4:-12031905 None:intergenic
CTACTTCATTTGCAACTTTG+TGG 0.404030 4.4:+12031855 MS.gene27953:CDS
GTTGGACCTTTAACACTGTT+GGG 0.416893 4.4:+12031398 MS.gene27953:CDS
TACTTCATTTGCAACTTTGT+GGG 0.443056 4.4:+12031856 MS.gene27953:CDS
AAGATCAGATCAGGCTTGCT+AGG 0.444325 4.4:+12031824 MS.gene27953:CDS
TTTGTGGGTCACTGCGAGTC+CGG 0.457246 4.4:+12031871 MS.gene27953:CDS
CTAGTAGAGAAACATGGCTC+TGG 0.459629 4.4:+12031282 None:intergenic
CAAGAGGAGCAAAAGTGTAT+AGG 0.485165 4.4:+12031794 MS.gene27953:CDS
GGTTGGACCTTTAACACTGT+TGG 0.487153 4.4:+12031397 MS.gene27953:CDS
GGACAGGAAAAGATCAGATC+AGG 0.488069 4.4:+12031815 MS.gene27953:CDS
AGAAACATGGCTCTGGGAAG+AGG 0.491666 4.4:+12031289 None:intergenic
GGAGCAAAAGTGTATAGGAC+AGG 0.499425 4.4:+12031799 MS.gene27953:CDS
GTGTTAAAGGTCCAACCTCC+AGG 0.508532 4.4:-12031391 None:intergenic
GCTGCTACCTACACTGTTGG+AGG 0.517016 4.4:+12031367 MS.gene27953:CDS
CATGCTGCTACCTACACTGT+TGG 0.528640 4.4:+12031364 MS.gene27953:CDS
AGATCAGATCAGGCTTGCTA+GGG 0.531401 4.4:+12031825 MS.gene27953:CDS
CTGTTGGAGGTCCTGGAGGT+TGG 0.534102 4.4:+12031380 MS.gene27953:CDS
TTTAGGGCTGGTGACACACT+TGG 0.537054 4.4:+12031439 MS.gene27953:CDS
TCTGGGAAGAGGCAGTGCAT+TGG 0.544752 4.4:+12031300 MS.gene27953:CDS
AATGGAAAACGCTTTAGGGC+TGG 0.547621 4.4:+12031427 MS.gene27953:CDS
GCCACCACATTGTGAGCTGA+TGG 0.559088 4.4:-12031736 None:intergenic
GGACCTTTAACACTGTTGGG+TGG 0.562616 4.4:+12031401 MS.gene27953:CDS
ATCAGCTCACAATGTGGTGG+CGG 0.571374 4.4:+12031738 MS.gene27953:CDS
TAGTAGAGAAACATGGCTCT+GGG 0.578678 4.4:+12031283 None:intergenic
AATGTGGTGGCGGTGAACAA+AGG 0.599948 4.4:+12031748 MS.gene27953:CDS
GTGGTGGCGGTGAACAAAGG+AGG 0.602014 4.4:+12031751 MS.gene27953:CDS
TACACTGTTGGAGGTCCTGG+AGG 0.611395 4.4:+12031376 MS.gene27953:CDS
GATAGTTGCAAGACACCAAG+AGG 0.632266 4.4:+12031778 MS.gene27953:CDS
TCCATCAGCTCACAATGTGG+TGG 0.645459 4.4:+12031735 MS.gene27953:CDS
TGGTGGCGGTGAACAAAGGA+GGG 0.647438 4.4:+12031752 MS.gene27953:CDS
GATCAGATCAGGCTTGCTAG+GGG 0.679726 4.4:+12031826 MS.gene27953:CDS
TCCAGGACCTCCAACAGTGT+AGG 0.688043 4.4:-12031374 None:intergenic
TAGTCCATCAGCTCACAATG+TGG 0.719459 4.4:+12031732 MS.gene27953:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTAAGTACACTCTT+CGG + chr4.4:12031610-12031629 MS.gene27953:intron 20.0%
TACTTCATTTGCAACTTTGT+GGG + chr4.4:12031856-12031875 MS.gene27953:CDS 30.0%
TCTTCAAAACTCTCTTTCAT+CGG + chr4.4:12031587-12031606 MS.gene27953:intron 30.0%
!! ATTGATGGCAATTTTCATTC+CGG - chr4.4:12031893-12031912 None:intergenic 30.0%
ACTCCAAACATTACTTCATC+TGG + chr4.4:12031515-12031534 MS.gene27953:intron 35.0%
AGACCAGATGAAGTAATGTT+TGG - chr4.4:12031521-12031540 None:intergenic 35.0%
CTACTTCATTTGCAACTTTG+TGG + chr4.4:12031855-12031874 MS.gene27953:CDS 35.0%
GCCTAATGGAAAACGCTTTA+GGG + chr4.4:12031423-12031442 MS.gene27953:CDS 40.0%
GTTGGACCTTTAACACTGTT+GGG + chr4.4:12031398-12031417 MS.gene27953:CDS 40.0%
! CAAGAGGAGCAAAAGTGTAT+AGG + chr4.4:12031794-12031813 MS.gene27953:CDS 40.0%
! CTATACACTTTTGCTCCTCT+TGG - chr4.4:12031796-12031815 None:intergenic 40.0%
GATAGTTGCAAGACACCAAG+AGG + chr4.4:12031778-12031797 MS.gene27953:CDS 45.0%
GGACAGGAAAAGATCAGATC+AGG + chr4.4:12031815-12031834 MS.gene27953:CDS 45.0%
GGCCTAATGGAAAACGCTTT+AGG + chr4.4:12031422-12031441 MS.gene27953:CDS 45.0%
GGTTGGACCTTTAACACTGT+TGG + chr4.4:12031397-12031416 MS.gene27953:CDS 45.0%
TAGTCCATCAGCTCACAATG+TGG + chr4.4:12031732-12031751 MS.gene27953:CDS 45.0%
! AAGATCAGATCAGGCTTGCT+AGG + chr4.4:12031824-12031843 MS.gene27953:CDS 45.0%
! GGAGCAAAAGTGTATAGGAC+AGG + chr4.4:12031799-12031818 MS.gene27953:CDS 45.0%
!! AATGGAAAACGCTTTAGGGC+TGG + chr4.4:12031427-12031446 MS.gene27953:CDS 45.0%
!! AGATCAGATCAGGCTTGCTA+GGG + chr4.4:12031825-12031844 MS.gene27953:CDS 45.0%
!! GCCCTAAAGCGTTTTCCATT+AGG - chr4.4:12031427-12031446 None:intergenic 45.0%
AACACTGTTGGGTGGCCTAA+TGG + chr4.4:12031409-12031428 MS.gene27953:CDS 50.0%
AGGCCACCCAACAGTGTTAA+AGG - chr4.4:12031407-12031426 None:intergenic 50.0%
ATCAGCTCACAATGTGGTGG+CGG + chr4.4:12031738-12031757 MS.gene27953:CDS 50.0%
CATGCTGCTACCTACACTGT+TGG + chr4.4:12031364-12031383 MS.gene27953:CDS 50.0%
GGACCTTTAACACTGTTGGG+TGG + chr4.4:12031401-12031420 MS.gene27953:CDS 50.0%
GTGTTAAAGGTCCAACCTCC+AGG - chr4.4:12031394-12031413 None:intergenic 50.0%
TCCATCAGCTCACAATGTGG+TGG + chr4.4:12031735-12031754 MS.gene27953:CDS 50.0%
! TTTAGGGCTGGTGACACACT+TGG + chr4.4:12031439-12031458 MS.gene27953:CDS 50.0%
!! AATGTGGTGGCGGTGAACAA+AGG + chr4.4:12031748-12031767 MS.gene27953:CDS 50.0%
!! GATCAGATCAGGCTTGCTAG+GGG + chr4.4:12031826-12031845 MS.gene27953:CDS 50.0%
ACCTACACTGTTGGAGGTCC+TGG + chr4.4:12031373-12031392 MS.gene27953:CDS 55.0%
GCCACCACATTGTGAGCTGA+TGG - chr4.4:12031739-12031758 None:intergenic 55.0%
GCTGCTACCTACACTGTTGG+AGG + chr4.4:12031367-12031386 MS.gene27953:CDS 55.0%
TACACTGTTGGAGGTCCTGG+AGG + chr4.4:12031376-12031395 MS.gene27953:CDS 55.0%
TCCAGGACCTCCAACAGTGT+AGG - chr4.4:12031377-12031396 None:intergenic 55.0%
TCTGGGAAGAGGCAGTGCAT+TGG + chr4.4:12031300-12031319 MS.gene27953:CDS 55.0%
TTTGTGGGTCACTGCGAGTC+CGG + chr4.4:12031871-12031890 MS.gene27953:CDS 55.0%
!! TGGTGGCGGTGAACAAAGGA+GGG + chr4.4:12031752-12031771 MS.gene27953:CDS 55.0%
CTGTTGGAGGTCCTGGAGGT+TGG + chr4.4:12031380-12031399 MS.gene27953:CDS 60.0%
!! GTGGTGGCGGTGAACAAAGG+AGG + chr4.4:12031751-12031770 MS.gene27953:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 12031295 12031921 12031295 ID=MS.gene27953
chr4.4 mRNA 12031295 12031921 12031295 ID=MS.gene27953.t1;Parent=MS.gene27953
chr4.4 exon 12031295 12031460 12031295 ID=MS.gene27953.t1.exon1;Parent=MS.gene27953.t1
chr4.4 CDS 12031295 12031460 12031295 ID=cds.MS.gene27953.t1;Parent=MS.gene27953.t1
chr4.4 exon 12031722 12031921 12031722 ID=MS.gene27953.t1.exon2;Parent=MS.gene27953.t1
chr4.4 CDS 12031722 12031921 12031722 ID=cds.MS.gene27953.t1;Parent=MS.gene27953.t1
Gene Sequence

>MS.gene27953

ATGGCTCTGGGAAGAGGCAGTGCATTGGTTCTACTAGTCTGCTTTTTTGTTCTTAACTCTGAGCTTGCTCATGCTGCTACCTACACTGTTGGAGGTCCTGGAGGTTGGACCTTTAACACTGTTGGGTGGCCTAATGGAAAACGCTTTAGGGCTGGTGACACACTTGGTATGTATCTAGTATATTCTAATGTTATTAAATTTCACTCTAAATATGAATCTTACTCCAAACATTACTTCATCTGGTCTTATTTATAAGAGTGCGCAAATTCTCACTTTAACACTGTTTTTTTCTTCTTCAAAACTCTCTTTCATCGGATAAAATTAAGTACACTCTTCGGTCACTATTATAAGTAAAAATTTATATTTTAGATTTATTCAATTAATGATGTATATGATCTTTATTATAAATTTGTGTATGTTTGTGCAGTGTTCAACTATAGTCCATCAGCTCACAATGTGGTGGCGGTGAACAAAGGAGGGTATGATAGTTGCAAGACACCAAGAGGAGCAAAAGTGTATAGGACAGGAAAAGATCAGATCAGGCTTGCTAGGGGACAAAACTACTTCATTTGCAACTTTGTGGGTCACTGCGAGTCCGGAATGAAAATTGCCATCAATGCTGCTTAA

Protein sequence

>MS.gene27953.t1

MALGRGSALVLLVCFFVLNSELAHAATYTVGGPGGWTFNTVGWPNGKRFRAGDTLVFNYSPSAHNVVAVNKGGYDSCKTPRGAKVYRTGKDQIRLARGQNYFICNFVGHCESGMKIAINAA