Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28036.t1 | KEH20784.1 | 93.3 | 149 | 10 | 0 | 1 | 149 | 1 | 149 | 9.10E-71 | 276.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28036.t1 | A0A072TTE4 | 93.3 | 149 | 10 | 0 | 1 | 149 | 1 | 149 | 6.6e-71 | 276.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060808 | MS.gene28036 | 0.802561 | 5.29E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28036.t1 | MTR_8g089920 | 93.289 | 149 | 10 | 0 | 1 | 149 | 1 | 149 | 7.49e-102 | 288 |
| MS.gene28036.t1 | MTR_3g086830 | 43.137 | 153 | 75 | 4 | 1 | 143 | 4 | 154 | 3.35e-23 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATGGTTAATTTCAATTA+TGG | 0.201378 | 4.4:-11107045 | MS.gene28036:CDS |
| TGAACGTGTGAAAGCTTCTT+TGG | 0.251848 | 4.4:+11106888 | None:intergenic |
| TGATTTCCCTATCTCTGATT+TGG | 0.302555 | 4.4:+11106983 | None:intergenic |
| CAAGAAGCCAAATCAGAGAT+AGG | 0.355674 | 4.4:-11106990 | MS.gene28036:CDS |
| ATCATAAGAAAAGAAGCTTT+TGG | 0.366357 | 4.4:-11106942 | MS.gene28036:CDS |
| GAGAAGGTTTGAGGAGATTG+AGG | 0.374689 | 4.4:+11107153 | None:intergenic |
| AATCTGTCACACTAGAAGTT+TGG | 0.436538 | 4.4:+11107079 | None:intergenic |
| GATTTGGCTTCTTGAATCAA+AGG | 0.476574 | 4.4:+11106999 | None:intergenic |
| AATTGCTGAGGTTGAAGAGA+AGG | 0.495615 | 4.4:+11107137 | None:intergenic |
| GAGGTTGAAGAGAAGGTTTG+AGG | 0.504838 | 4.4:+11107144 | None:intergenic |
| AAGAAGCTTTCACACGTTCA+AGG | 0.506620 | 4.4:-11106885 | MS.gene28036:CDS |
| TAGAAGTTTGGCGGCGGTGA+CGG | 0.511622 | 4.4:+11107091 | None:intergenic |
| GCTACTCAAATTGACAAGAA+AGG | 0.520563 | 4.4:-11106914 | MS.gene28036:CDS |
| TTGTCAGAGTTAGAAGCAGC+AGG | 0.525527 | 4.4:-11107282 | MS.gene28036:CDS |
| GGAAATCAATGGAGATAACC+AGG | 0.535671 | 4.4:-11106968 | MS.gene28036:CDS |
| AAGAAGCCAAATCAGAGATA+GGG | 0.536255 | 4.4:-11106989 | MS.gene28036:CDS |
| ATCAGAGATAGGGAAATCAA+TGG | 0.542074 | 4.4:-11106979 | MS.gene28036:CDS |
| AAGTTTGGCGGCGGTGACGG+CGG | 0.545140 | 4.4:+11107094 | None:intergenic |
| CACACGTTCAAGGACAGTGA+GGG | 0.564456 | 4.4:-11106875 | MS.gene28036:CDS |
| CTGTCACACTAGAAGTTTGG+CGG | 0.564777 | 4.4:+11107082 | None:intergenic |
| TCAGCAATTTATATGTCTCA+TGG | 0.564801 | 4.4:-11107123 | MS.gene28036:CDS |
| TCAAATGACAAACAACTACC+TGG | 0.600195 | 4.4:-11107240 | MS.gene28036:CDS |
| TCACACTAGAAGTTTGGCGG+CGG | 0.649605 | 4.4:+11107085 | None:intergenic |
| CACGTTCAAGGACAGTGAGG+GGG | 0.652331 | 4.4:-11106873 | MS.gene28036:CDS |
| ATGAGACATATAAATTGCTG+AGG | 0.658796 | 4.4:+11107125 | None:intergenic |
| GAAATCAATGGAGATAACCA+GGG | 0.659361 | 4.4:-11106967 | MS.gene28036:CDS |
| ACACGTTCAAGGACAGTGAG+GGG | 0.663586 | 4.4:-11106874 | MS.gene28036:CDS |
| AAGCAATAACAACAAACACC+AGG | 0.676498 | 4.4:+11107222 | None:intergenic |
| TCACACGTTCAAGGACAGTG+AGG | 0.684808 | 4.4:-11106876 | MS.gene28036:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATGGTTAATTTCAATTA+TGG | - | chr4.4:11107104-11107123 | MS.gene28036:CDS | 15.0% |
| !!! | AGATTCTTTTTCTTCAAATA+TGG | - | chr4.4:11107088-11107107 | MS.gene28036:CDS | 20.0% |
| !! | ATCATAAGAAAAGAAGCTTT+TGG | - | chr4.4:11107207-11107226 | MS.gene28036:CDS | 25.0% |
| ATGAGACATATAAATTGCTG+AGG | + | chr4.4:11107027-11107046 | None:intergenic | 30.0% | |
| TCAGCAATTTATATGTCTCA+TGG | - | chr4.4:11107026-11107045 | MS.gene28036:CDS | 30.0% | |
| ! | TTCTTTTCTTATGATTTCCC+TGG | + | chr4.4:11107202-11107221 | None:intergenic | 30.0% |
| AAGAAGCCAAATCAGAGATA+GGG | - | chr4.4:11107160-11107179 | MS.gene28036:CDS | 35.0% | |
| AAGCAATAACAACAAACACC+AGG | + | chr4.4:11106930-11106949 | None:intergenic | 35.0% | |
| AATCTGTCACACTAGAAGTT+TGG | + | chr4.4:11107073-11107092 | None:intergenic | 35.0% | |
| ATCAGAGATAGGGAAATCAA+TGG | - | chr4.4:11107170-11107189 | MS.gene28036:CDS | 35.0% | |
| GAAATCAATGGAGATAACCA+GGG | - | chr4.4:11107182-11107201 | MS.gene28036:CDS | 35.0% | |
| GCTACTCAAATTGACAAGAA+AGG | - | chr4.4:11107235-11107254 | MS.gene28036:CDS | 35.0% | |
| TCAAATGACAAACAACTACC+TGG | - | chr4.4:11106909-11106928 | MS.gene28036:CDS | 35.0% | |
| TGATTTCCCTATCTCTGATT+TGG | + | chr4.4:11107169-11107188 | None:intergenic | 35.0% | |
| ! | GATTTGGCTTCTTGAATCAA+AGG | + | chr4.4:11107153-11107172 | None:intergenic | 35.0% |
| AAGAAGCTTTCACACGTTCA+AGG | - | chr4.4:11107264-11107283 | MS.gene28036:CDS | 40.0% | |
| AATTGCTGAGGTTGAAGAGA+AGG | + | chr4.4:11107015-11107034 | None:intergenic | 40.0% | |
| CAAGAAGCCAAATCAGAGAT+AGG | - | chr4.4:11107159-11107178 | MS.gene28036:CDS | 40.0% | |
| GGAAATCAATGGAGATAACC+AGG | - | chr4.4:11107181-11107200 | MS.gene28036:CDS | 40.0% | |
| TGAACGTGTGAAAGCTTCTT+TGG | + | chr4.4:11107264-11107283 | None:intergenic | 40.0% | |
| CTGTCACACTAGAAGTTTGG+CGG | + | chr4.4:11107070-11107089 | None:intergenic | 45.0% | |
| GAGAAGGTTTGAGGAGATTG+AGG | + | chr4.4:11106999-11107018 | None:intergenic | 45.0% | |
| GAGGTTGAAGAGAAGGTTTG+AGG | + | chr4.4:11107008-11107027 | None:intergenic | 45.0% | |
| TTGTCAGAGTTAGAAGCAGC+AGG | - | chr4.4:11106867-11106886 | MS.gene28036:CDS | 45.0% | |
| ACACGTTCAAGGACAGTGAG+GGG | - | chr4.4:11107275-11107294 | MS.gene28036:CDS | 50.0% | |
| CACACGTTCAAGGACAGTGA+GGG | - | chr4.4:11107274-11107293 | MS.gene28036:CDS | 50.0% | |
| TCACACGTTCAAGGACAGTG+AGG | - | chr4.4:11107273-11107292 | MS.gene28036:CDS | 50.0% | |
| TCACACTAGAAGTTTGGCGG+CGG | + | chr4.4:11107067-11107086 | None:intergenic | 50.0% | |
| AAGGACAGTGAGGGGGAGAA+TGG | - | chr4.4:11107283-11107302 | MS.gene28036:CDS | 55.0% | |
| AGGACAGTGAGGGGGAGAAT+GGG | - | chr4.4:11107284-11107303 | MS.gene28036:CDS | 55.0% | |
| CACGTTCAAGGACAGTGAGG+GGG | - | chr4.4:11107276-11107295 | MS.gene28036:CDS | 55.0% | |
| TAGAAGTTTGGCGGCGGTGA+CGG | + | chr4.4:11107061-11107080 | None:intergenic | 55.0% | |
| ! | AAGTTTGGCGGCGGTGACGG+CGG | + | chr4.4:11107058-11107077 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 11106861 | 11107310 | 11106861 | ID=MS.gene28036 |
| chr4.4 | mRNA | 11106861 | 11107310 | 11106861 | ID=MS.gene28036.t1;Parent=MS.gene28036 |
| chr4.4 | exon | 11106861 | 11107310 | 11106861 | ID=MS.gene28036.t1.exon1;Parent=MS.gene28036.t1 |
| chr4.4 | CDS | 11106861 | 11107310 | 11106861 | ID=cds.MS.gene28036.t1;Parent=MS.gene28036.t1 |
| Gene Sequence |
| Protein sequence |
