Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28146.t1 | XP_024629482.1 | 89.7 | 725 | 39 | 4 | 630 | 1324 | 43 | 761 | 3.80E-293 | 1018.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28146.t1 | O94481 | 28.6 | 175 | 111 | 3 | 1138 | 1311 | 353 | 514 | 8.9e-09 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28146.t1 | A0A396GS65 | 89.4 | 727 | 39 | 4 | 630 | 1324 | 43 | 763 | 4.6e-293 | 1017.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene28146.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056403 | MS.gene28146 | 0.823638 | 1.24E-53 | -1.69E-46 |
| MS.gene056605 | MS.gene28146 | 0.817082 | 3.97E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28146.t1 | MTR_8g091830 | 90.838 | 764 | 17 | 7 | 591 | 1324 | 18 | 758 | 0.0 | 1234 |
| MS.gene28146.t1 | MTR_8g091830 | 66.903 | 423 | 117 | 9 | 118 | 520 | 326 | 745 | 2.04e-127 | 412 |
| MS.gene28146.t1 | MTR_8g091830 | 50.993 | 151 | 48 | 6 | 1 | 131 | 1 | 145 | 7.76e-21 | 99.8 |
| MS.gene28146.t1 | MTR_8g091830 | 90.777 | 759 | 15 | 7 | 591 | 1317 | 18 | 753 | 0.0 | 1217 |
| MS.gene28146.t1 | MTR_8g091830 | 65.517 | 435 | 127 | 9 | 118 | 532 | 328 | 759 | 2.53e-127 | 414 |
| MS.gene28146.t1 | MTR_8g091830 | 50.327 | 153 | 48 | 6 | 1 | 131 | 1 | 147 | 1.95e-20 | 98.6 |
| MS.gene28146.t1 | MTR_8g091850 | 76.842 | 285 | 21 | 2 | 157 | 441 | 55 | 294 | 8.52e-130 | 401 |
| MS.gene28146.t1 | MTR_8g091850 | 55.738 | 305 | 70 | 4 | 927 | 1230 | 53 | 293 | 1.30e-81 | 270 |
| MS.gene28146.t1 | MTR_4g067250 | 48.108 | 555 | 209 | 20 | 3 | 529 | 77 | 580 | 5.26e-107 | 351 |
| MS.gene28146.t1 | MTR_4g067250 | 54.115 | 401 | 145 | 11 | 931 | 1324 | 212 | 580 | 5.17e-95 | 318 |
| MS.gene28146.t1 | MTR_8g091840 | 92.857 | 56 | 3 | 1 | 470 | 524 | 5 | 60 | 4.42e-27 | 105 |
| MS.gene28146.t1 | MTR_8g091840 | 59.701 | 67 | 19 | 4 | 1260 | 1324 | 5 | 65 | 7.24e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28146.t1 | AT4G39160 | 28.261 | 552 | 312 | 16 | 733 | 1245 | 15 | 521 | 8.54e-44 | 169 |
| MS.gene28146.t1 | AT4G39160 | 37.826 | 230 | 126 | 7 | 236 | 454 | 298 | 521 | 5.91e-29 | 124 |
| MS.gene28146.t1 | AT4G39160 | 28.261 | 552 | 312 | 16 | 733 | 1245 | 15 | 521 | 8.54e-44 | 169 |
| MS.gene28146.t1 | AT4G39160 | 37.826 | 230 | 126 | 7 | 236 | 454 | 298 | 521 | 5.91e-29 | 124 |
| MS.gene28146.t1 | AT4G39160 | 28.261 | 552 | 312 | 16 | 733 | 1245 | 15 | 521 | 1.26e-43 | 169 |
| MS.gene28146.t1 | AT4G39160 | 37.826 | 230 | 126 | 7 | 236 | 454 | 298 | 521 | 9.22e-29 | 124 |
Find 255 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCAACAGCCTTGTTAAA+TGG | 0.149899 | 4.4:-9778565 | None:intergenic |
| CCAATATTCCTAACCCTTTC+CGG | 0.169829 | 4.4:-9784598 | None:intergenic |
| TGTTGATGCGCTTGTCTTCT+TGG | 0.180256 | 4.4:-9786048 | None:intergenic |
| TGCATTGGAATTTCAGATAT+TGG | 0.186110 | 4.4:-9777328 | None:intergenic |
| CCGATTCCTCTTCTCATTTC+CGG | 0.203819 | 4.4:+9783261 | MS.gene28146:CDS |
| CTTCTATTATCTTCAGAAAA+TGG | 0.204672 | 4.4:-9784871 | None:intergenic |
| TCGATCACCGATTTATAAAA+AGG | 0.211257 | 4.4:-9780401 | None:intergenic |
| GAAGAAATCAGTGGCTAAAA+AGG | 0.211844 | 4.4:+9786079 | MS.gene28146:CDS |
| TCCTAACCCTTTCCGGTTCC+AGG | 0.250680 | 4.4:-9784591 | None:intergenic |
| ATCAACGAACAATACCTATT+AGG | 0.275169 | 4.4:+9786325 | MS.gene28146:CDS |
| AAAGACTGGTTATTATATAA+AGG | 0.275999 | 4.4:-9779871 | None:intergenic |
| CCCTTTCCGGTTCCAGGATC+AGG | 0.297732 | 4.4:-9784585 | None:intergenic |
| ACTCTGAAGGCATAGCATTC+AGG | 0.302380 | 4.4:-9784927 | None:intergenic |
| TCTAGGTCGTGGCTTAATCT+TGG | 0.307370 | 4.4:-9784700 | None:intergenic |
| GATCTTCTATGCTTCTTTAG+AGG | 0.313417 | 4.4:-9778460 | None:intergenic |
| GACCCTCCTTATGTTATTGA+TGG | 0.314436 | 4.4:+9785978 | MS.gene28146:CDS |
| ATTGAATTACCAGTCTTTCA+TGG | 0.319869 | 4.4:+9787657 | MS.gene28146:CDS |
| TGGATCAATGCCAGATTTCA+TGG | 0.322835 | 4.4:-9785594 | None:intergenic |
| ACAGAAACAAACTTCTAATA+AGG | 0.327322 | 4.4:+9780427 | MS.gene28146:CDS |
| TCAGCCAATGTGGGACTATC+TGG | 0.334761 | 4.4:-9785656 | None:intergenic |
| ACAGAAACAAAATTCTAATA+AGG | 0.334895 | 4.4:+9788186 | MS.gene28146:CDS |
| GCGGGCTTCAAAAGCGCTTC+AGG | 0.341307 | 4.4:+9784366 | MS.gene28146:CDS |
| GACCCTCCTTATGAGTTTGA+TGG | 0.342811 | 4.4:+9778601 | MS.gene28146:CDS |
| TCAACGAACAATACCTATTA+GGG | 0.342822 | 4.4:+9786326 | MS.gene28146:CDS |
| AGACGATACCGAAGAGAAAA+AGG | 0.345945 | 4.4:+9777408 | MS.gene28146:CDS |
| AAGACTGGTTATTATATAAA+GGG | 0.352288 | 4.4:-9779870 | None:intergenic |
| GATGCCTCAGATTTAATGAT+AGG | 0.358895 | 4.4:+9788226 | MS.gene28146:CDS |
| ATATAGGCTATTAGGCAGTT+TGG | 0.359903 | 4.4:+9787861 | MS.gene28146:intron |
| AGTAAAATAATATCCCTAAT+AGG | 0.364611 | 4.4:-9786339 | None:intergenic |
| TCTTGACAGCAACAAGAGTT+TGG | 0.369143 | 4.4:+9784308 | MS.gene28146:CDS |
| CATGATATCAATCTTAATAA+TGG | 0.369196 | 4.4:+9776248 | MS.gene28146:CDS |
| CCTCAACAGCCTTGTTAAAT+GGG | 0.374079 | 4.4:-9778564 | None:intergenic |
| GGTGGTTTGGGCTTAGCCTT+AGG | 0.384423 | 4.4:-9783536 | None:intergenic |
| AATGAACCTTCCCCATTTGT+AGG | 0.385631 | 4.4:-9784285 | None:intergenic |
| CATAAAATATCTTCTATTGC+TGG | 0.387802 | 4.4:+9785849 | MS.gene28146:CDS |
| AAAGACTGGTAATTCAATAA+AGG | 0.392051 | 4.4:-9787652 | None:intergenic |
| CCCCGTTTCGATTTAACCTT+AGG | 0.393024 | 4.4:-9776146 | None:intergenic |
| TAAAGATCAAGAAGATTCTA+TGG | 0.395924 | 4.4:+9789031 | MS.gene28146:CDS |
| AGTGTTAGTGTACCTTTGAT+TGG | 0.396209 | 4.4:-9786516 | None:intergenic |
| TCAATGGGGCAGTCAGGATC+AGG | 0.398869 | 4.4:-9785548 | None:intergenic |
| TTTGTCAAGCCTTCATGCCT+TGG | 0.399449 | 4.4:-9785629 | None:intergenic |
| GCTTCTGCTTCTGCTTCATC+TGG | 0.399801 | 4.4:+9784744 | MS.gene28146:CDS |
| TGTGCGCAAACCTTAGTTCC+AGG | 0.400334 | 4.4:-9788268 | None:intergenic |
| AACAAAGAACTGCCTACAAA+TGG | 0.403333 | 4.4:+9784273 | MS.gene28146:CDS |
| AGTGATGTCCTCCCTGATCC+TGG | 0.404496 | 4.4:+9784573 | MS.gene28146:CDS |
| TCGTCAGATTGCTCTGGAAC+TGG | 0.405106 | 4.4:-9789059 | None:intergenic |
| CTAGGTCGTGGCTTAATCTT+GGG | 0.405320 | 4.4:-9784699 | None:intergenic |
| GGTTCATGAACAACGATCAT+AGG | 0.406073 | 4.4:-9781686 | None:intergenic |
| ATATAATAACCAGTCTTTCA+AGG | 0.406319 | 4.4:+9779876 | MS.gene28146:CDS |
| CAAACCTTAGTTCCAGGTGT+TGG | 0.407219 | 4.4:-9788262 | None:intergenic |
| CAACATAGAAGTTGAAGATC+AGG | 0.407390 | 4.4:+9781231 | MS.gene28146:CDS |
| CTTAAATCTTTCAATGTGAA+TGG | 0.409452 | 4.4:+9785342 | MS.gene28146:CDS |
| AGCATTATGAGAATCATGTT+TGG | 0.410582 | 4.4:-9783663 | None:intergenic |
| ACAAGGCACCTAGGGGAAAA+TGG | 0.411092 | 4.4:+9787680 | MS.gene28146:CDS |
| GATTATCAATCCATGAAATC+TGG | 0.414632 | 4.4:+9785584 | MS.gene28146:CDS |
| TCCCATGGAAAATCAAATTC+CGG | 0.417667 | 4.4:+9781258 | MS.gene28146:CDS |
| CATAGTGGACAAGGCTCTGC+TGG | 0.417669 | 4.4:+9778910 | MS.gene28146:intron |
| CATAGTGGACAAGGCTCTGC+TGG | 0.417669 | 4.4:+9786287 | MS.gene28146:intron |
| AGAGGTTGCAACAGCTGAGC+AGG | 0.420001 | 4.4:+9788989 | MS.gene28146:CDS |
| ACCTAAGGTTAAATCGAAAC+GGG | 0.420062 | 4.4:+9776145 | MS.gene28146:CDS |
| CTCTGAAGGCATAGCATTCA+GGG | 0.422534 | 4.4:-9784926 | None:intergenic |
| TGCCTTGGAATTTCAGATGT+TGG | 0.423028 | 4.4:-9785614 | None:intergenic |
| GGCTAATGATGATTTAGAAA+AGG | 0.423170 | 4.4:+9778750 | MS.gene28146:CDS |
| GCATTATGAGAATCATGTTT+GGG | 0.424036 | 4.4:-9783662 | None:intergenic |
| CAAAAGTAGGTCGGAAATAC+AGG | 0.424081 | 4.4:+9785401 | MS.gene28146:CDS |
| GAGAGGCAGGTTTGCGCCTA+AGG | 0.424149 | 4.4:+9783520 | MS.gene28146:CDS |
| AAACCTTAGTTCCAGGTGTT+GGG | 0.426302 | 4.4:-9788261 | None:intergenic |
| GTCCAATGCCATTTGTTCTC+AGG | 0.429782 | 4.4:-9781664 | None:intergenic |
| CATAGAAGATCTCCTATTGC+TGG | 0.429813 | 4.4:+9778472 | MS.gene28146:CDS |
| AGGTGGCAAGTTTGCACCTA+AGG | 0.436477 | 4.4:+9776130 | MS.gene28146:CDS |
| ATTGCTGGTGTGGAGGATAA+AGG | 0.436946 | 4.4:+9778487 | MS.gene28146:CDS |
| ACAAGACACCTAGGGCAAAA+TGG | 0.437516 | 4.4:+9779899 | MS.gene28146:CDS |
| ACAAAGAACTGCCTACAAAT+GGG | 0.438146 | 4.4:+9784274 | MS.gene28146:CDS |
| GTCAAAGCTTCTTCATGCAT+TGG | 0.440699 | 4.4:-9777343 | None:intergenic |
| CACCTAAGGTTAAATCGAAA+CGG | 0.441683 | 4.4:+9776144 | MS.gene28146:CDS |
| ACATTGAAAGATTTAAGATC+TGG | 0.441714 | 4.4:-9785336 | None:intergenic |
| TGTGGATACCTCAGGAATAT+CGG | 0.442586 | 4.4:-9781726 | None:intergenic |
| TTATCCTCCACACCAGCAAT+AGG | 0.442858 | 4.4:-9778484 | None:intergenic |
| GCTTTGAACAATCATGCAAC+AGG | 0.445071 | 4.4:+9780226 | MS.gene28146:CDS |
| GTCCTCCCTGATCCTGGAAC+CGG | 0.446592 | 4.4:+9784579 | MS.gene28146:CDS |
| CCGGAAATGAGAAGAGGAAT+CGG | 0.449413 | 4.4:-9783261 | None:intergenic |
| CAGTTCTTCCATCCTACCTA+TGG | 0.454716 | 4.4:-9777200 | None:intergenic |
| CCTGATCCTGGAACCGGAAA+GGG | 0.454744 | 4.4:+9784585 | MS.gene28146:CDS |
| GGACCCAACACCTGGAACTA+AGG | 0.455739 | 4.4:+9788258 | MS.gene28146:CDS |
| ATGGTTAAGCGAGTAGTTGA+TGG | 0.457692 | 4.4:-9777181 | None:intergenic |
| GATGCCTCGGATTTCATGCC+AGG | 0.457944 | 4.4:+9780470 | MS.gene28146:CDS |
| AAAGGTTGCAACAGCTGAGC+AGG | 0.459179 | 4.4:+9781207 | MS.gene28146:CDS |
| CTACTTAGCCTGAGAACAAA+TGG | 0.460151 | 4.4:+9781656 | MS.gene28146:CDS |
| ACTGATTTACCAAAGCTAGA+AGG | 0.460360 | 4.4:-9784979 | None:intergenic |
| GCTAATGATGATTTAGAAAA+GGG | 0.461578 | 4.4:+9778751 | MS.gene28146:CDS |
| GCCATGAATAATCGTGGAAA+AGG | 0.463451 | 4.4:+9787990 | MS.gene28146:CDS |
| GGATTTCATGCCAGGGGAGG+TGG | 0.466274 | 4.4:+9780478 | MS.gene28146:CDS |
| ACATTGTCAATGGGGCAGTC+AGG | 0.469696 | 4.4:-9785554 | None:intergenic |
| GGGTCTTCAACCACCTCCCC+TGG | 0.469715 | 4.4:-9780488 | None:intergenic |
| TGCAACAGCTGAGCAGGAGC+AGG | 0.470277 | 4.4:+9788995 | MS.gene28146:CDS |
| ATTGCTGGTGTGGAGGATAA+AGG | 0.470324 | 4.4:+9785864 | MS.gene28146:CDS |
| GTGTCTTGTCCTTGAAAGAC+TGG | 0.471184 | 4.4:-9779885 | None:intergenic |
| TCATCATAGACATTGTCAAT+GGG | 0.474029 | 4.4:-9785563 | None:intergenic |
| CCCATTTAACAAGGCTGTTG+AGG | 0.477110 | 4.4:+9778564 | MS.gene28146:CDS |
| TCATTGAATGAGGCTGTTAA+CGG | 0.478665 | 4.4:+9785942 | MS.gene28146:CDS |
| GAGAATGAGGACCCAACACC+TGG | 0.480041 | 4.4:+9788250 | MS.gene28146:CDS |
| AATAACTTGCAAACTTTCGT+TGG | 0.480571 | 4.4:-9777254 | None:intergenic |
| TAGCATTCAGGGAGTCAGAA+TGG | 0.488808 | 4.4:-9784915 | None:intergenic |
| TCATCTCCATCAAACTCATA+AGG | 0.489388 | 4.4:-9778607 | None:intergenic |
| CAGTATCGTCTTGCTGCAAA+AGG | 0.489581 | 4.4:-9785679 | None:intergenic |
| TTCTGAAGATAATAGAAGCT+TGG | 0.493783 | 4.4:+9784876 | MS.gene28146:CDS |
| ATCATCATAGACATTGTCAA+TGG | 0.494759 | 4.4:-9785564 | None:intergenic |
| TTCTTCGCCGAGATGAATGC+GGG | 0.496692 | 4.4:-9778798 | None:intergenic |
| CGGAAATGAGAAGAGGAATC+GGG | 0.500025 | 4.4:-9783260 | None:intergenic |
| ATTCTCAACCATGATGCAAC+TGG | 0.501051 | 4.4:+9785126 | MS.gene28146:CDS |
| TTGAATTGTTCTCGACCTGC+CGG | 0.502242 | 4.4:+9783186 | MS.gene28146:CDS |
| TGACGTAGTGTACCTTTGAT+TGG | 0.502991 | 4.4:-9779147 | None:intergenic |
| AACCATGATGCAACTGGTAC+AGG | 0.505460 | 4.4:+9785132 | MS.gene28146:CDS |
| CTTGATCTTTAACATTGATG+TGG | 0.506223 | 4.4:-9789019 | None:intergenic |
| GGCTAATGATGATTCAGAAA+AGG | 0.506523 | 4.4:+9786127 | MS.gene28146:CDS |
| TCATCTCCATCAATAACATA+AGG | 0.510363 | 4.4:-9785984 | None:intergenic |
| TCACTATCGTCAGATTGCTC+TGG | 0.510890 | 4.4:-9789065 | None:intergenic |
| CTTGTCTGCGGAAATCGAGC+TGG | 0.511468 | 4.4:+9784542 | MS.gene28146:CDS |
| TATTAGACGTGCAGAACGTA+TGG | 0.514226 | 4.4:+9778976 | MS.gene28146:CDS |
| ATACATTAGTTCCATGTGTC+GGG | 0.514518 | 4.4:-9780508 | None:intergenic |
| TTTCATGGACAAGGCACCTA+GGG | 0.515232 | 4.4:+9787672 | MS.gene28146:CDS |
| TAACATTTACCACGTGTGAC+AGG | 0.515968 | 4.4:-9776020 | None:intergenic |
| AATAAGGAAGAAGATACTGC+TGG | 0.517268 | 4.4:+9788202 | MS.gene28146:CDS |
| ATGCCTCAGATTTAATGATA+GGG | 0.518508 | 4.4:+9788227 | MS.gene28146:CDS |
| CCCTGATCCTGGAACCGGAA+AGG | 0.518602 | 4.4:+9784584 | MS.gene28146:CDS |
| GACCTGCCGGAAAACGCGAC+CGG | 0.520846 | 4.4:+9783199 | MS.gene28146:CDS |
| TGATAATTATCAGACCTCAT+AGG | 0.521077 | 4.4:-9776227 | None:intergenic |
| AAACGGGGAATTTCATCTAA+AGG | 0.522905 | 4.4:+9776161 | MS.gene28146:CDS |
| GCAGAAGCAGAAGCAGTAGC+TGG | 0.523053 | 4.4:-9784735 | None:intergenic |
| ATGATACAACTGAGTTTCCC+TGG | 0.524533 | 4.4:+9780130 | MS.gene28146:CDS |
| GTGCCTTGTCCATGAAAGAC+TGG | 0.528971 | 4.4:-9787666 | None:intergenic |
| CATCGAACATTACCTCTTCG+AGG | 0.531532 | 4.4:+9778950 | MS.gene28146:CDS |
| GCATCATATAACCACTGTAA+AGG | 0.531639 | 4.4:-9780205 | None:intergenic |
| ATGGCATCAGTTAACAGTAA+AGG | 0.533326 | 4.4:-9787972 | None:intergenic |
| AGCATTCAGGGAGTCAGAAT+GGG | 0.534554 | 4.4:-9784914 | None:intergenic |
| TTTAATGATAGGGGAGAATG+AGG | 0.536462 | 4.4:+9788237 | MS.gene28146:CDS |
| AAAAGTTCCCGCATTCATCT+CGG | 0.541414 | 4.4:+9778791 | MS.gene28146:CDS |
| CCTTTCCGGTTCCAGGATCA+GGG | 0.543738 | 4.4:-9784584 | None:intergenic |
| CGGGATACGATGGCAAAAGT+AGG | 0.544927 | 4.4:+9785388 | MS.gene28146:CDS |
| TAGAGATGAATTTAGAGACC+TGG | 0.547077 | 4.4:+9787588 | MS.gene28146:CDS |
| GCTGATATCGATGTCAATAA+CGG | 0.547205 | 4.4:+9783683 | MS.gene28146:CDS |
| CTGCAAAAGGTCAGCCAATG+TGG | 0.547743 | 4.4:-9785666 | None:intergenic |
| CATACATTAGTTCCATGTGT+CGG | 0.547764 | 4.4:-9780509 | None:intergenic |
| TGCAGAACATAAGGAGCGGT+TGG | 0.551127 | 4.4:+9786362 | MS.gene28146:CDS |
| AATATCTTCTATTGCTGGTG+TGG | 0.552691 | 4.4:+9785854 | MS.gene28146:CDS |
| GTGTACCTTTGATTGGTGGA+AGG | 0.552725 | 4.4:-9786509 | None:intergenic |
| CTTTCATGGACAAGGCACCT+AGG | 0.554899 | 4.4:+9787671 | MS.gene28146:CDS |
| AGCCTGAGAACAAATGGCAT+TGG | 0.555984 | 4.4:+9781662 | MS.gene28146:CDS |
| TCACTATCATCAGATTGCTC+CGG | 0.557302 | 4.4:-9781277 | None:intergenic |
| CACCTGTACCAGTTGCATCA+TGG | 0.562328 | 4.4:-9785134 | None:intergenic |
| TGGCTCGATCATCATCATCC+AGG | 0.563395 | 4.4:-9787606 | None:intergenic |
| TCTGAAATTCCAAGGCATGA+AGG | 0.563472 | 4.4:+9785620 | MS.gene28146:CDS |
| ATATCGGAGGAAAGATAAGA+TGG | 0.563579 | 4.4:-9781710 | None:intergenic |
| AAGATCTCCTATTGCTGGTG+TGG | 0.563647 | 4.4:+9778477 | MS.gene28146:CDS |
| ATCTCCTATTGCTGGTGTGG+AGG | 0.564088 | 4.4:+9778480 | MS.gene28146:CDS |
| TCGCTTAACCATAGGTAGGA+TGG | 0.564291 | 4.4:+9777192 | MS.gene28146:CDS |
| GTTTCCGGCGAGCGAAACTC+CGG | 0.565422 | 4.4:-9783218 | None:intergenic |
| ATCTTCTATTGCTGGTGTGG+AGG | 0.566330 | 4.4:+9785857 | MS.gene28146:CDS |
| CTTCAACTGAAGAACCTATG+AGG | 0.569557 | 4.4:+9776213 | MS.gene28146:CDS |
| TGAAGATCAGGAAGATCCCA+TGG | 0.569852 | 4.4:+9781243 | MS.gene28146:CDS |
| TTTATATGCATGCAGTGACA+GGG | 0.572330 | 4.4:+9779759 | MS.gene28146:intron |
| TTTCTTCGCCGAGATGAATG+CGG | 0.572858 | 4.4:-9778799 | None:intergenic |
| CGTAGTGTACCTTTGATTGG+TGG | 0.573053 | 4.4:-9779144 | None:intergenic |
| GTTAGTGTACCTTTGATTGG+TGG | 0.573797 | 4.4:-9786513 | None:intergenic |
| ATCCAACATCTGAAATTCCA+AGG | 0.574184 | 4.4:+9785612 | MS.gene28146:CDS |
| AGATACCGCTAGAGATGCCT+CGG | 0.577005 | 4.4:+9780457 | MS.gene28146:CDS |
| CTCCCCTATCATTAAATCTG+AGG | 0.577536 | 4.4:-9788230 | None:intergenic |
| AAATGATGATGAGGACGAAG+TGG | 0.578250 | 4.4:+9778639 | MS.gene28146:CDS |
| TGCAGAACGTATGGAGCGAT+CGG | 0.579460 | 4.4:+9778985 | MS.gene28146:CDS |
| TCTCCATCAATAACATAAGG+AGG | 0.580123 | 4.4:-9785981 | None:intergenic |
| TCGTGAACTGCAGACTCTGA+AGG | 0.582141 | 4.4:-9784940 | None:intergenic |
| GCGACCGGAGTTTCGCTCGC+CGG | 0.582555 | 4.4:+9783214 | MS.gene28146:CDS |
| TCAACTACTCGCTTAACCAT+AGG | 0.587367 | 4.4:+9777184 | MS.gene28146:CDS |
| AGACTGGTTATTATATAAAG+GGG | 0.587808 | 4.4:-9779869 | None:intergenic |
| TCTCCATCAAACTCATAAGG+AGG | 0.588456 | 4.4:-9778604 | None:intergenic |
| TGCCTCAGATTTAATGATAG+GGG | 0.588626 | 4.4:+9788228 | MS.gene28146:CDS |
| CGAGGATGCTAATAATGAAG+AGG | 0.589996 | 4.4:+9779792 | MS.gene28146:CDS |
| TGAGTTTGGAGAAGTGCGAG+AGG | 0.590260 | 4.4:+9784434 | MS.gene28146:CDS |
| GTCATCGAGTGATTCTAGCC+CGG | 0.591218 | 4.4:-9785103 | None:intergenic |
| CTCGGATTTCATGCCAGGGG+AGG | 0.592720 | 4.4:+9780475 | MS.gene28146:CDS |
| CTTTGGTAAATCAGTTGCAG+AGG | 0.593038 | 4.4:+9784987 | MS.gene28146:CDS |
| GCTGGTGTATTGCTGACTCT+AGG | 0.593908 | 4.4:-9784717 | None:intergenic |
| AAACATCTTCCACCAATCAA+AGG | 0.593927 | 4.4:+9779135 | MS.gene28146:CDS |
| CAATCTGACGATAGTGACGA+TGG | 0.595092 | 4.4:+9789071 | MS.gene28146:CDS |
| AATTAAGTTGAAGTACAAGA+AGG | 0.595648 | 4.4:+9787935 | MS.gene28146:CDS |
| TTTGTCCTTCCGGGATACGA+TGG | 0.597883 | 4.4:+9785378 | MS.gene28146:CDS |
| AGATACTGAACTGTTCTATG+AGG | 0.599256 | 4.4:+9779930 | MS.gene28146:CDS |
| TACAAAATAATGAAGACGCA+TGG | 0.600991 | 4.4:+9789096 | MS.gene28146:CDS |
| CTGGTTATTATATAAAGGGG+TGG | 0.601015 | 4.4:-9779866 | None:intergenic |
| TTTCTTCGTCGAGATGCATG+GGG | 0.601698 | 4.4:-9786176 | None:intergenic |
| TCTTTCCTCCGATATTCCTG+AGG | 0.602906 | 4.4:+9781718 | MS.gene28146:CDS |
| GGGGAATTTCATCTAAAGGT+AGG | 0.604367 | 4.4:+9776165 | MS.gene28146:CDS |
| AAACACCTTCCACCAATCAA+AGG | 0.604763 | 4.4:+9786504 | MS.gene28146:CDS |
| CAATAACATAAGGAGGGTCG+AGG | 0.606753 | 4.4:-9785974 | None:intergenic |
| CTTTCAAGGACAAGACACCT+AGG | 0.606828 | 4.4:+9779890 | MS.gene28146:CDS |
| CTCCATCAAACTCATAAGGA+GGG | 0.607122 | 4.4:-9778603 | None:intergenic |
| GCTAGAAGTCAGCCTAAACG+TGG | 0.607960 | 4.4:+9777285 | MS.gene28146:CDS |
| ATAAAGGTGGTAAAACCTCA+AGG | 0.609003 | 4.4:+9785880 | MS.gene28146:CDS |
| ATCTTTCAATGTGAATGGCG+CGG | 0.609959 | 4.4:+9785347 | MS.gene28146:CDS |
| TTAGGTGCAAACTTGCCACC+TGG | 0.610561 | 4.4:-9776128 | None:intergenic |
| ATGCCTCGGATTTCATGCCA+GGG | 0.613701 | 4.4:+9780471 | MS.gene28146:CDS |
| ACTGATGCCATGAATAATCG+TGG | 0.614136 | 4.4:+9787984 | MS.gene28146:CDS |
| GTGATGATGAGTTACAAAGA+TGG | 0.615643 | 4.4:+9781296 | MS.gene28146:CDS |
| GGCTGATGCTAATTATGAAG+AGG | 0.617871 | 4.4:+9787540 | MS.gene28146:CDS |
| CTCCATCAATAACATAAGGA+GGG | 0.625247 | 4.4:-9785980 | None:intergenic |
| TGCAAAAGGTCAGCCAATGT+GGG | 0.626313 | 4.4:-9785665 | None:intergenic |
| ATAAAGGTAGTAAAACCTCA+AGG | 0.627073 | 4.4:+9778503 | MS.gene28146:CDS |
| TTGGTGGAAGGTGTTTGCGA+AGG | 0.627136 | 4.4:-9786497 | None:intergenic |
| CAAACTCATAAGGAGGGTCG+AGG | 0.627209 | 4.4:-9778597 | None:intergenic |
| GTGGTTGAAGACCCGACACA+TGG | 0.629822 | 4.4:+9780497 | MS.gene28146:CDS |
| CAATGGGGCAGTCAGGATCA+GGG | 0.632141 | 4.4:-9785547 | None:intergenic |
| CAAAGAACTGCCTACAAATG+GGG | 0.632541 | 4.4:+9784275 | MS.gene28146:CDS |
| TTATGTTCAGGTCTTGTCTG+CGG | 0.632639 | 4.4:+9784530 | MS.gene28146:intron |
| TTACCAGTCTTTCATGGACA+AGG | 0.633575 | 4.4:+9787663 | MS.gene28146:CDS |
| TAGTTGAGATTCTTTGTGAG+TGG | 0.635988 | 4.4:-9783633 | None:intergenic |
| TTCTGCAGATAGTCCCACAT+TGG | 0.636578 | 4.4:+9777369 | MS.gene28146:CDS |
| TTCTCCAGATAGTCCCACAT+TGG | 0.636578 | 4.4:+9785652 | MS.gene28146:CDS |
| GTATTGCTGACTCTAGGTCG+TGG | 0.639699 | 4.4:-9784711 | None:intergenic |
| GAACTGCCTACAAATGGGGA+AGG | 0.640951 | 4.4:+9784279 | MS.gene28146:CDS |
| GCGCTACCGGAAATGAGAAG+AGG | 0.643266 | 4.4:-9783267 | None:intergenic |
| TTCAGGGAGTCAGAATGGGA+AGG | 0.643999 | 4.4:-9784910 | None:intergenic |
| CTACTCGCTTAACCATAGGT+AGG | 0.644131 | 4.4:+9777188 | MS.gene28146:CDS |
| CTCCCCTGGCATGAAATCCG+AGG | 0.647352 | 4.4:-9780474 | None:intergenic |
| TCAGGGAGGACATCACTGAA+AGG | 0.647641 | 4.4:-9784567 | None:intergenic |
| AATATCTATATGCAGTGATG+GGG | 0.653102 | 4.4:+9787486 | MS.gene28146:intron |
| AGAACGTATGGAGCGATCGG+CGG | 0.656384 | 4.4:+9778988 | MS.gene28146:CDS |
| GAAATCCGAGGCATCTCTAG+CGG | 0.659596 | 4.4:-9780462 | None:intergenic |
| TCAGAGTCTGCAGTTCACGA+TGG | 0.663364 | 4.4:+9784943 | MS.gene28146:CDS |
| CGTCTAATAATACCTCGAAG+AGG | 0.664501 | 4.4:-9778962 | None:intergenic |
| TTTCAAGGACAAGACACCTA+GGG | 0.665399 | 4.4:+9779891 | MS.gene28146:CDS |
| AATCTGACGATAGTGACGAT+GGG | 0.666315 | 4.4:+9789072 | MS.gene28146:CDS |
| CATCATAGACATTGTCAATG+GGG | 0.666547 | 4.4:-9785562 | None:intergenic |
| TACTTGCAGAACATAAGGAG+CGG | 0.669846 | 4.4:+9786358 | MS.gene28146:CDS |
| TCTGATGCTCCTGTCACACG+TGG | 0.672401 | 4.4:+9776011 | MS.gene28146:CDS |
| TTATATGCATGCAGTGACAG+GGG | 0.675034 | 4.4:+9779760 | MS.gene28146:intron |
| AGCGGCAACATCCTTTACAG+TGG | 0.676327 | 4.4:+9780194 | MS.gene28146:CDS |
| CTAGAGATTAGCCTAAACGC+GGG | 0.676355 | 4.4:+9784348 | MS.gene28146:CDS |
| TTTCAATGTGAATGGCGCGG+AGG | 0.676830 | 4.4:+9785350 | MS.gene28146:CDS |
| ATACGATGGCAAAAGTAGGT+CGG | 0.677358 | 4.4:+9785392 | MS.gene28146:CDS |
| ACTTGAAGAAAATGATGATG+AGG | 0.680891 | 4.4:+9778630 | MS.gene28146:CDS |
| TGCCTCGGATTTCATGCCAG+GGG | 0.682068 | 4.4:+9780472 | MS.gene28146:CDS |
| TTCTTCGTCGAGATGCATGG+GGG | 0.683443 | 4.4:-9786175 | None:intergenic |
| TGAAGTACAAGAAAGAAGAG+CGG | 0.690863 | 4.4:+9780176 | MS.gene28146:CDS |
| CCTAAGGTTAAATCGAAACG+GGG | 0.691493 | 4.4:+9776146 | MS.gene28146:CDS |
| ACTAGAGATTAGCCTAAACG+CGG | 0.695442 | 4.4:+9784347 | MS.gene28146:CDS |
| GCTTCAGGCGACATTAACAA+TGG | 0.697080 | 4.4:+9784381 | MS.gene28146:CDS |
| AAGTTCAAAGAAGAAATCAG+TGG | 0.701783 | 4.4:+9778693 | MS.gene28146:CDS |
| AAGTTCAAAGAAGAAATCAG+TGG | 0.701783 | 4.4:+9786070 | MS.gene28146:CDS |
| TCGGAGGAAAGATAAGATGG+TGG | 0.701969 | 4.4:-9781707 | None:intergenic |
| TTCATGGACAAGGCACCTAG+GGG | 0.703716 | 4.4:+9787673 | MS.gene28146:CDS |
| CTAGAAGTCAGCCTAAACGT+GGG | 0.712351 | 4.4:+9777286 | MS.gene28146:CDS |
| TTCCGGTTCCAGGATCAGGG+AGG | 0.714895 | 4.4:-9784581 | None:intergenic |
| GGATACCTCAGGAATATCGG+AGG | 0.719269 | 4.4:-9781723 | None:intergenic |
| GCTGGTGTGGAGGATAAAGG+TGG | 0.724198 | 4.4:+9785867 | MS.gene28146:CDS |
| AAATGACGATGAGAACGACG+TGG | 0.754814 | 4.4:+9786016 | MS.gene28146:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 9775984 | 9789142 | 9775984 | ID=MS.gene28146 |
| chr4.4 | mRNA | 9775984 | 9789142 | 9775984 | ID=MS.gene28146.t1;Parent=MS.gene28146 |
| chr4.4 | exon | 9775984 | 9776032 | 9775984 | ID=MS.gene28146.t1.exon1;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9775984 | 9776032 | 9775984 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9776123 | 9776269 | 9776123 | ID=MS.gene28146.t1.exon2;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9776123 | 9776269 | 9776123 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9777155 | 9777429 | 9777155 | ID=MS.gene28146.t1.exon3;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9777155 | 9777429 | 9777155 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9778439 | 9778829 | 9778439 | ID=MS.gene28146.t1.exon4;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9778439 | 9778829 | 9778439 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9778915 | 9779009 | 9778915 | ID=MS.gene28146.t1.exon5;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9778915 | 9779009 | 9778915 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9779113 | 9779156 | 9779113 | ID=MS.gene28146.t1.exon6;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9779113 | 9779156 | 9779113 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9779774 | 9779951 | 9779774 | ID=MS.gene28146.t1.exon7;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9779774 | 9779951 | 9779774 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9780103 | 9780247 | 9780103 | ID=MS.gene28146.t1.exon8;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9780103 | 9780247 | 9780103 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9780399 | 9780526 | 9780399 | ID=MS.gene28146.t1.exon9;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9780399 | 9780526 | 9780399 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9781205 | 9781317 | 9781205 | ID=MS.gene28146.t1.exon10;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9781205 | 9781317 | 9781205 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9781637 | 9781739 | 9781637 | ID=MS.gene28146.t1.exon11;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9781637 | 9781739 | 9781637 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9783180 | 9783282 | 9783180 | ID=MS.gene28146.t1.exon12;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9783180 | 9783282 | 9783180 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9783519 | 9783704 | 9783519 | ID=MS.gene28146.t1.exon13;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9783519 | 9783704 | 9783519 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9784268 | 9784455 | 9784268 | ID=MS.gene28146.t1.exon14;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9784268 | 9784455 | 9784268 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9784540 | 9784606 | 9784540 | ID=MS.gene28146.t1.exon15;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9784540 | 9784606 | 9784540 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9784685 | 9784765 | 9784685 | ID=MS.gene28146.t1.exon16;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9784685 | 9784765 | 9784685 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9784866 | 9785008 | 9784866 | ID=MS.gene28146.t1.exon17;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9784866 | 9785008 | 9784866 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9785087 | 9785153 | 9785087 | ID=MS.gene28146.t1.exon18;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9785087 | 9785153 | 9785087 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9785331 | 9785422 | 9785331 | ID=MS.gene28146.t1.exon19;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9785331 | 9785422 | 9785331 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9785530 | 9785712 | 9785530 | ID=MS.gene28146.t1.exon20;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9785530 | 9785712 | 9785530 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9785807 | 9786206 | 9785807 | ID=MS.gene28146.t1.exon21;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9785807 | 9786206 | 9785807 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9786292 | 9786386 | 9786292 | ID=MS.gene28146.t1.exon22;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9786292 | 9786386 | 9786292 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9786482 | 9786525 | 9786482 | ID=MS.gene28146.t1.exon23;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9786482 | 9786525 | 9786482 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9787501 | 9787732 | 9787501 | ID=MS.gene28146.t1.exon24;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9787501 | 9787732 | 9787501 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9787867 | 9788011 | 9787867 | ID=MS.gene28146.t1.exon25;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9787867 | 9788011 | 9787867 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9788155 | 9788279 | 9788155 | ID=MS.gene28146.t1.exon26;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9788155 | 9788279 | 9788155 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| chr4.4 | exon | 9788987 | 9789142 | 9788987 | ID=MS.gene28146.t1.exon27;Parent=MS.gene28146.t1 |
| chr4.4 | CDS | 9788987 | 9789142 | 9788987 | ID=cds.MS.gene28146.t1;Parent=MS.gene28146.t1 |
| Gene Sequence |
| Protein sequence |
