Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28349.t1 | XP_013456284.1 | 88.4 | 653 | 40 | 3 | 1 | 624 | 1 | 646 | 0.00E+00 | 1097.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28349.t1 | A0A072UWT7 | 88.4 | 653 | 40 | 3 | 1 | 624 | 1 | 646 | 0.0e+00 | 1097.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053144 | MS.gene28349 | 0.826759 | 2.27E-54 | -1.69E-46 |
| MS.gene054584 | MS.gene28349 | 0.817624 | 2.99E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene28349 | 0.807087 | 6.00E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene28349 | 0.800472 | 1.42E-48 | -1.69E-46 |
| MS.gene055580 | MS.gene28349 | -0.819581 | 1.08E-52 | -1.69E-46 |
| MS.gene056043 | MS.gene28349 | -0.806044 | 9.96E-50 | -1.69E-46 |
| MS.gene056368 | MS.gene28349 | 0.907867 | 3.18E-81 | -1.69E-46 |
| MS.gene059154 | MS.gene28349 | 0.818678 | 1.73E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28349.t1 | MTR_4g067090 | 88.668 | 653 | 38 | 4 | 1 | 624 | 1 | 646 | 0.0 | 1165 |
| MS.gene28349.t1 | MTR_2g098830 | 51.360 | 662 | 273 | 8 | 2 | 620 | 644 | 1299 | 0.0 | 623 |
| MS.gene28349.t1 | MTR_2g098830 | 40.000 | 155 | 54 | 4 | 271 | 388 | 8 | 160 | 2.89e-19 | 93.2 |
| MS.gene28349.t1 | MTR_2g098830 | 36.905 | 168 | 97 | 4 | 333 | 496 | 482 | 644 | 5.44e-17 | 85.9 |
| MS.gene28349.t1 | MTR_2g098830 | 51.360 | 662 | 273 | 8 | 2 | 620 | 911 | 1566 | 0.0 | 623 |
| MS.gene28349.t1 | MTR_2g098830 | 57.110 | 429 | 134 | 7 | 5 | 388 | 4 | 427 | 1.74e-146 | 462 |
| MS.gene28349.t1 | MTR_2g098830 | 36.905 | 168 | 97 | 4 | 333 | 496 | 749 | 911 | 2.97e-17 | 86.7 |
| MS.gene28349.t1 | MTR_2g098710 | 60.714 | 364 | 130 | 4 | 5 | 360 | 3 | 361 | 1.92e-145 | 428 |
| MS.gene28349.t1 | MTR_2g098720 | 62.029 | 345 | 124 | 3 | 4 | 348 | 3 | 340 | 7.45e-143 | 421 |
| MS.gene28349.t1 | MTR_2g098730 | 63.072 | 306 | 111 | 1 | 5 | 310 | 4 | 307 | 2.74e-129 | 387 |
| MS.gene28349.t1 | MTR_0002s1280 | 54.545 | 341 | 110 | 4 | 5 | 342 | 4 | 302 | 5.15e-115 | 347 |
| MS.gene28349.t1 | MTR_2g098770 | 46.765 | 340 | 99 | 5 | 5 | 341 | 4 | 264 | 5.31e-85 | 271 |
| MS.gene28349.t1 | MTR_2g098740 | 52.308 | 65 | 31 | 0 | 2 | 66 | 20 | 84 | 8.91e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28349.t1 | AT1G05950 | 32.404 | 574 | 298 | 17 | 4 | 553 | 13 | 520 | 1.23e-74 | 249 |
| MS.gene28349.t1 | AT1G05950 | 32.404 | 574 | 298 | 17 | 4 | 553 | 13 | 520 | 1.23e-74 | 249 |
| MS.gene28349.t1 | AT1G05950 | 32.236 | 577 | 301 | 17 | 1 | 553 | 10 | 520 | 1.46e-74 | 249 |
| MS.gene28349.t1 | AT1G05950 | 32.404 | 574 | 298 | 17 | 4 | 553 | 13 | 520 | 6.28e-74 | 249 |
| MS.gene28349.t1 | AT1G05950 | 32.404 | 574 | 298 | 17 | 4 | 553 | 13 | 520 | 6.70e-74 | 249 |
Find 111 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGAATCTTGAAGACTTT+GGG | 0.158603 | 4.2:+38740076 | None:intergenic |
| CTGTTGAGACTTCAGAATTT+TGG | 0.161594 | 4.2:+38743073 | None:intergenic |
| CAGCTATCATTGACAGAAAA+AGG | 0.162964 | 4.2:-38743285 | MS.gene28349:CDS |
| GGTTCTGAGGCCACCTTTAT+TGG | 0.191132 | 4.2:-38743364 | MS.gene28349:CDS |
| CCTTCTTGCTGTCGATTAAA+AGG | 0.199021 | 4.2:+38743172 | None:intergenic |
| TAACACTGATATTATGCTTT+TGG | 0.222359 | 4.2:-38742513 | MS.gene28349:CDS |
| AACTTGCAAAATTCGTTAAA+TGG | 0.228149 | 4.2:+38743393 | None:intergenic |
| TTATCTCACAGGGGATTTCT+AGG | 0.264933 | 4.2:-38741759 | MS.gene28349:intron |
| TGGCTCTGGCAATGCTATTC+AGG | 0.269758 | 4.2:-38740684 | MS.gene28349:intron |
| AAGGACAGAAACTTTCGATA+TGG | 0.276928 | 4.2:+38743191 | None:intergenic |
| GTTGAATAAACTGAGAAGTA+TGG | 0.279829 | 4.2:-38735896 | MS.gene28349:CDS |
| ATGGGCCATCCCTCGATATT+AGG | 0.291937 | 4.2:+38743210 | None:intergenic |
| ATCACCAATGCTATACAATT+TGG | 0.297771 | 4.2:-38740752 | MS.gene28349:CDS |
| TGTGAGATAACTTCAGAATA+AGG | 0.310344 | 4.2:+38741772 | None:intergenic |
| GTATAGCATTGGTGATCTTC+TGG | 0.317782 | 4.2:+38740759 | None:intergenic |
| ATAAGTAAGGACTTGGATTC+TGG | 0.324800 | 4.2:-38740938 | MS.gene28349:CDS |
| AATGGCAGATATTCTAGTTC+TGG | 0.328231 | 4.2:+38743411 | None:intergenic |
| ATATAGGACTCTGTCACTTC+AGG | 0.328571 | 4.2:+38740959 | None:intergenic |
| GCATAGCTTGCAAGGTCCTT+TGG | 0.341619 | 4.2:-38741854 | MS.gene28349:intron |
| TCCAACTGAGGATGCAATTC+AGG | 0.359131 | 4.2:-38744088 | MS.gene28349:CDS |
| TCTTTGAATCTTGAAGACTT+TGG | 0.360035 | 4.2:+38740075 | None:intergenic |
| ATTTGTTCACATCCATTTCA+TGG | 0.360457 | 4.2:+38740816 | None:intergenic |
| ATCAATGATAGAATGCATTA+TGG | 0.363892 | 4.2:-38737925 | MS.gene28349:CDS |
| TCAGGTGTTTCTACAACATT+TGG | 0.364760 | 4.2:-38744070 | MS.gene28349:CDS |
| TGCAAGGTCCTTTGGTCAAA+AGG | 0.391451 | 4.2:-38741846 | MS.gene28349:CDS |
| TTTACAAGATTTGAGGGTAG+TGG | 0.401313 | 4.2:-38741005 | MS.gene28349:CDS |
| AATTTCATATATGCCAATAA+AGG | 0.402771 | 4.2:+38743351 | None:intergenic |
| CCTTCAGCAGCGCAAGATAG+AGG | 0.405235 | 4.2:-38739372 | MS.gene28349:CDS |
| ATCCAAGTCCTTACTTATAT+AGG | 0.408398 | 4.2:+38740943 | None:intergenic |
| TAACCTCCTGATTGATTGAC+TGG | 0.422854 | 4.2:+38742423 | None:intergenic |
| TATATGCTTCCAACATTACT+TGG | 0.425020 | 4.2:+38735864 | None:intergenic |
| CAAATTCTGAGAATGTTAAC+TGG | 0.435144 | 4.2:-38739326 | MS.gene28349:intron |
| CATATAGTTTACAAGATTTG+AGG | 0.436673 | 4.2:-38741012 | MS.gene28349:CDS |
| ACAGCAAGTAATTGCCAAAC+AGG | 0.437795 | 4.2:-38743992 | MS.gene28349:intron |
| CTCTATCTTGCGCTGCTGAA+GGG | 0.440040 | 4.2:+38739373 | None:intergenic |
| GTTCCATCACTGAGGAGATA+TGG | 0.441755 | 4.2:-38743128 | MS.gene28349:CDS |
| CCTCTATCTTGCGCTGCTGA+AGG | 0.443269 | 4.2:+38739372 | None:intergenic |
| AATAGCATTGCCAGAGCCAT+TGG | 0.446449 | 4.2:+38740688 | None:intergenic |
| AAGAAGAAAACACACCTGTT+TGG | 0.450078 | 4.2:+38743978 | None:intergenic |
| TATCATGGAGACCCATGAAA+TGG | 0.452413 | 4.2:-38740828 | MS.gene28349:CDS |
| CCTTTCAATTACATCCTTGA+GGG | 0.460597 | 4.2:+38742391 | None:intergenic |
| AGGACAGAAACTTTCGATAT+GGG | 0.463069 | 4.2:+38743192 | None:intergenic |
| TATAGGACTCTGTCACTTCA+GGG | 0.467012 | 4.2:+38740960 | None:intergenic |
| TATACAATTTGGTGAACATC+AGG | 0.472255 | 4.2:-38740741 | MS.gene28349:CDS |
| TTGAATAAACTGAGAAGTAT+GGG | 0.480086 | 4.2:-38735895 | MS.gene28349:CDS |
| TTCACCAAATTGTATAGCAT+TGG | 0.480139 | 4.2:+38740748 | None:intergenic |
| GTCCTATATAAGTAAGGACT+TGG | 0.486129 | 4.2:-38740945 | MS.gene28349:CDS |
| GACAGAGTCCTATATAAGTA+AGG | 0.487508 | 4.2:-38740951 | MS.gene28349:CDS |
| ACAAGGATGCTCAACGGTAA+AGG | 0.491827 | 4.2:-38740224 | MS.gene28349:CDS |
| TATCTTCTCTAATAAGAAAG+AGG | 0.494834 | 4.2:-38740034 | MS.gene28349:CDS |
| CAGTTTAAATCCAATGGCTC+TGG | 0.497458 | 4.2:-38740698 | MS.gene28349:CDS |
| TTATTCTGAAGTTATCTCAC+AGG | 0.498048 | 4.2:-38741770 | MS.gene28349:CDS |
| GTGCTCCCAGTCAATCAATC+AGG | 0.501419 | 4.2:-38742429 | MS.gene28349:CDS |
| TTTGAATCTTGAAGACTTTG+GGG | 0.502496 | 4.2:+38740077 | None:intergenic |
| ATATAGTTTACAAGATTTGA+GGG | 0.508018 | 4.2:-38741011 | MS.gene28349:CDS |
| TTGGATTTAAACTGCACAGA+TGG | 0.508608 | 4.2:+38740707 | None:intergenic |
| TTTGTTCACATCCATTTCAT+GGG | 0.518510 | 4.2:+38740817 | None:intergenic |
| TTCTCTTGCTTCAAGAGGAG+TGG | 0.519482 | 4.2:+38735936 | None:intergenic |
| GTTTATATTAACAGTTACAT+TGG | 0.520185 | 4.2:+38735993 | None:intergenic |
| TCTCTTGCTTCAAGAGGAGT+GGG | 0.522171 | 4.2:+38735937 | None:intergenic |
| CCCTCAAGGATGTAATTGAA+AGG | 0.522915 | 4.2:-38742391 | MS.gene28349:intron |
| TCAAAAGGAGTTCTAGCAGT+TGG | 0.526233 | 4.2:-38741831 | MS.gene28349:CDS |
| GAGTTAGAATGATTTGAACT+TGG | 0.533019 | 4.2:+38740113 | None:intergenic |
| CTATTCTCAAAGCAAAGAAA+AGG | 0.537022 | 4.2:-38742477 | MS.gene28349:CDS |
| ATTTCAATATCACCCTCACT+TGG | 0.540894 | 4.2:+38740902 | None:intergenic |
| AAGTTTAAGTGTTCCGATGA+GGG | 0.544204 | 4.2:-38735970 | MS.gene28349:CDS |
| GTAATAAATCAAGAAGGGAC+AGG | 0.545367 | 4.2:-38737882 | MS.gene28349:intron |
| ACCTGAATTGCATCCTCAGT+TGG | 0.546001 | 4.2:+38744087 | None:intergenic |
| TAGACCATGTGATTCTATCA+TGG | 0.549522 | 4.2:-38740843 | MS.gene28349:CDS |
| GCTGATTCTCTTGCTTCAAG+AGG | 0.555429 | 4.2:+38735931 | None:intergenic |
| GCAAGTTGATTGTGGTTCTG+AGG | 0.556738 | 4.2:-38743377 | MS.gene28349:CDS |
| ACCTTTCAATTACATCCTTG+AGG | 0.562218 | 4.2:+38742390 | None:intergenic |
| AATAAGAAAGAGGAATGAGC+TGG | 0.567307 | 4.2:-38740024 | MS.gene28349:intron |
| TACTTGCTGTTGAGAAGCTG+TGG | 0.567536 | 4.2:+38744005 | None:intergenic |
| GGAAGGCTGCAATGATGTAT+CGG | 0.573097 | 4.2:-38737904 | MS.gene28349:CDS |
| CTATGAAGATTGACTCGACA+AGG | 0.577961 | 4.2:-38740241 | MS.gene28349:CDS |
| CTTCAGCAGCGCAAGATAGA+GGG | 0.579807 | 4.2:-38739371 | MS.gene28349:CDS |
| ACATGGAAGTAAACCACCAC+AGG | 0.580384 | 4.2:+38741799 | None:intergenic |
| CTTCAGAATAAGGAAGCACA+TGG | 0.581249 | 4.2:+38741782 | None:intergenic |
| TATTCTGAAGTTATCTCACA+GGG | 0.582725 | 4.2:-38741769 | MS.gene28349:CDS |
| ATGATAGAATGCATTATGGA+AGG | 0.584757 | 4.2:-38737921 | MS.gene28349:CDS |
| GGTTATTAAAGTTCCCCTCA+AGG | 0.585039 | 4.2:-38742405 | MS.gene28349:CDS |
| TCTGTGCAGTTTAAATCCAA+TGG | 0.586104 | 4.2:-38740704 | MS.gene28349:CDS |
| TTCATATATGCCAATAAAGG+TGG | 0.587022 | 4.2:+38743354 | None:intergenic |
| GTATCGGTAATAAATCAAGA+AGG | 0.589608 | 4.2:-38737888 | MS.gene28349:CDS |
| CAAGTTTAAGTGTTCCGATG+AGG | 0.593108 | 4.2:-38735971 | MS.gene28349:CDS |
| TCTGCTGTGAAGGAAGCTGC+AGG | 0.595596 | 4.2:-38742955 | MS.gene28349:intron |
| CTTTCAATTACATCCTTGAG+GGG | 0.598422 | 4.2:+38742392 | None:intergenic |
| GGGACACAAGTTACCCTCAT+CGG | 0.598530 | 4.2:+38735957 | None:intergenic |
| AAAGAATGTTCCTAATATCG+AGG | 0.599060 | 4.2:-38743220 | MS.gene28349:CDS |
| GGACCATATCTCCTCAGTGA+TGG | 0.601464 | 4.2:+38743125 | None:intergenic |
| TATTTATAGTAAACCAAGTG+AGG | 0.603401 | 4.2:-38740915 | MS.gene28349:CDS |
| TGAAGTAGAGCTCATAGATG+TGG | 0.603411 | 4.2:-38743313 | MS.gene28349:CDS |
| AAGAATGTTCCTAATATCGA+GGG | 0.604182 | 4.2:-38743219 | MS.gene28349:CDS |
| AGTCGGGTATTCTGCTGTGA+AGG | 0.608334 | 4.2:-38742965 | MS.gene28349:CDS |
| TATCGGTAATAAATCAAGAA+GGG | 0.616879 | 4.2:-38737887 | MS.gene28349:CDS |
| TTGGACGATCACTCCTGTGG+TGG | 0.625303 | 4.2:-38741812 | MS.gene28349:CDS |
| AACCTCCTGATTGATTGACT+GGG | 0.625426 | 4.2:+38742424 | None:intergenic |
| CATGAGCAGAAAGAGAACAA+TGG | 0.627353 | 4.2:-38740878 | MS.gene28349:CDS |
| ACTTGCTGTTGAGAAGCTGT+GGG | 0.635516 | 4.2:+38744006 | None:intergenic |
| CACTGAGGAGATATGGTCCG+TGG | 0.640115 | 4.2:-38743121 | MS.gene28349:CDS |
| CTCCCAGTCAATCAATCAGG+AGG | 0.644545 | 4.2:-38742426 | MS.gene28349:CDS |
| GACTCGACAAGGATGCTCAA+CGG | 0.644800 | 4.2:-38740230 | MS.gene28349:CDS |
| ATTTATAGTAAACCAAGTGA+GGG | 0.646293 | 4.2:-38740914 | MS.gene28349:CDS |
| GCTGTTTGGTTCCATCACTG+AGG | 0.651655 | 4.2:-38743136 | MS.gene28349:CDS |
| ATGTTCCTAATATCGAGGGA+TGG | 0.652051 | 4.2:-38743215 | MS.gene28349:CDS |
| AGGATGCTCAACGGTAAAGG+CGG | 0.660364 | 4.2:-38740221 | MS.gene28349:CDS |
| CAGTTGGACGATCACTCCTG+TGG | 0.662817 | 4.2:-38741815 | MS.gene28349:CDS |
| ATCAGATGTGTGTCCAACTG+AGG | 0.695565 | 4.2:-38744100 | MS.gene28349:CDS |
| GTCTCCATGATAGAATCACA+TGG | 0.715164 | 4.2:+38740839 | None:intergenic |
| ATTCTGAAGTTATCTCACAG+GGG | 0.731508 | 4.2:-38741768 | MS.gene28349:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 38735879 | 38744133 | 38735879 | ID=MS.gene28349 |
| chr4.2 | mRNA | 38735879 | 38744133 | 38735879 | ID=MS.gene28349.t1;Parent=MS.gene28349 |
| chr4.2 | exon | 38743993 | 38744133 | 38743993 | ID=MS.gene28349.t1.exon1;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38743993 | 38744133 | 38743993 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| chr4.2 | exon | 38742956 | 38743481 | 38742956 | ID=MS.gene28349.t1.exon2;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38742956 | 38743481 | 38742956 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| chr4.2 | exon | 38742392 | 38742542 | 38742392 | ID=MS.gene28349.t1.exon3;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38742392 | 38742542 | 38742392 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| chr4.2 | exon | 38741760 | 38741870 | 38741760 | ID=MS.gene28349.t1.exon4;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38741760 | 38741870 | 38741760 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| chr4.2 | exon | 38740685 | 38741045 | 38740685 | ID=MS.gene28349.t1.exon5;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38740685 | 38741045 | 38740685 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| chr4.2 | exon | 38740025 | 38740264 | 38740025 | ID=MS.gene28349.t1.exon6;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38740025 | 38740264 | 38740025 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| chr4.2 | exon | 38739327 | 38739396 | 38739327 | ID=MS.gene28349.t1.exon7;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38739327 | 38739396 | 38739327 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| chr4.2 | exon | 38737883 | 38737966 | 38737883 | ID=MS.gene28349.t1.exon8;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38737883 | 38737966 | 38737883 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| chr4.2 | exon | 38735879 | 38736069 | 38735879 | ID=MS.gene28349.t1.exon9;Parent=MS.gene28349.t1 |
| chr4.2 | CDS | 38735879 | 38736069 | 38735879 | ID=cds.MS.gene28349.t1;Parent=MS.gene28349.t1 |
| Gene Sequence |
| Protein sequence |
