Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28463.t1 | RHN42128.1 | 100 | 120 | 0 | 0 | 1 | 120 | 1 | 120 | 1.40E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28463.t1 | Q42404 | 81.4 | 118 | 22 | 0 | 3 | 120 | 6 | 123 | 3.0e-49 | 195.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28463.t1 | G7LEU2 | 100.0 | 120 | 0 | 0 | 1 | 120 | 1 | 120 | 1.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060349 | MS.gene28463 | 0.804231 | 2.39E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28463.t1 | MTR_8g077840 | 100.000 | 120 | 0 | 0 | 1 | 120 | 1 | 120 | 9.50e-83 | 250 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28463.t1 | AT3G50670 | 81.356 | 118 | 22 | 0 | 3 | 120 | 6 | 123 | 2.65e-66 | 199 |
| MS.gene28463.t1 | AT3G50670 | 81.356 | 118 | 22 | 0 | 3 | 120 | 6 | 123 | 7.56e-65 | 202 |
Find 0 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTCGTATATTTTTTA+TGG | - | 15146:2660-2679 | MS.gene28463:intron | 10.0% |
| !! | AATTAACTAATCTAATATCT+AGG | + | 15146:2161-2180 | None:intergenic | 15.0% |
| !! | ATTAACTAATCTAATATCTA+GGG | + | 15146:2160-2179 | None:intergenic | 15.0% |
| !!! | TTGTTTATGTTTTTTGTTTA+GGG | - | 15146:2422-2441 | MS.gene28463:intron | 15.0% |
| !! | CGTTAATTATGAAGAAAATT+AGG | + | 15146:2088-2107 | None:intergenic | 20.0% |
| !! | TTAAAAGATAAGAACATTAC+AGG | + | 15146:2523-2542 | None:intergenic | 20.0% |
| !! | TTATATTCTCAACTAATATG+TGG | + | 15146:3010-3029 | None:intergenic | 20.0% |
| !!! | AGAGTTTGAAGTTATTTTAT+GGG | - | 15146:2685-2704 | MS.gene28463:intron | 20.0% |
| !!! | CTTGTTTATGTTTTTTGTTT+AGG | - | 15146:2421-2440 | MS.gene28463:intron | 20.0% |
| !!! | GTTTGATTATTGAATTTGTT+AGG | - | 15146:2340-2359 | MS.gene28463:intron | 20.0% |
| !!! | TTGTTGATTTTCAATAGTTA+GGG | - | 15146:2120-2139 | MS.gene28463:intron | 20.0% |
| !!! | TTTGTTGATTTTCAATAGTT+AGG | - | 15146:2119-2138 | MS.gene28463:intron | 20.0% |
| ! | AAATTAACAACGAAAAGTGA+GGG | + | 15146:2063-2082 | None:intergenic | 25.0% |
| ! | AATAATCAAACACCTGTTAA+CGG | + | 15146:2331-2350 | None:intergenic | 25.0% |
| ! | ATGACCACAAAACATTTATT+CGG | + | 15146:2972-2991 | None:intergenic | 25.0% |
| ! | CAAAACATTTATTCGGATTT+CGG | + | 15146:2965-2984 | None:intergenic | 25.0% |
| ! | CATATAGTTAATGTAGCAAT+CGG | - | 15146:3158-3177 | MS.gene28463:intron | 25.0% |
| ! | CTTCAAATTACTTAAACAAG+TGG | - | 15146:3070-3089 | MS.gene28463:intron | 25.0% |
| ! | TAAGTAATTTGAAGAGAAAC+TGG | + | 15146:3064-3083 | None:intergenic | 25.0% |
| !! | AAATCCGAATAAATGTTTTG+TGG | - | 15146:2965-2984 | MS.gene28463:intron | 25.0% |
| !! | TTAACGTAACTGTTTTCATA+AGG | + | 15146:2775-2794 | None:intergenic | 25.0% |
| !!! | CAGAGTTTGAAGTTATTTTA+TGG | - | 15146:2684-2703 | MS.gene28463:intron | 25.0% |
| !!! | TCTTTTAAGACTAATTATGC+TGG | - | 15146:2535-2554 | MS.gene28463:intron | 25.0% |
| !!! | TTATGTTTTTTGTTTAGGGA+TGG | - | 15146:2426-2445 | MS.gene28463:intron | 25.0% |
| ACTCTTCACTATTGAAACAA+GGG | + | 15146:3219-3238 | None:intergenic | 30.0% | |
| ATTAGTACCATACAAAATGC+AGG | + | 15146:2925-2944 | None:intergenic | 30.0% | |
| CAAATTAACAACGAAAAGTG+AGG | + | 15146:2064-2083 | None:intergenic | 30.0% | |
| GTGGTTATTATAATGTACAG+TGG | - | 15146:3089-3108 | MS.gene28463:intron | 30.0% | |
| TACTCTTCACTATTGAAACA+AGG | + | 15146:3220-3239 | None:intergenic | 30.0% | |
| TATGGTACTAATTCGTTCTT+AGG | - | 15146:2933-2952 | MS.gene28463:intron | 30.0% | |
| ! | GAATAAATGTTTTGTGGTCA+TGG | - | 15146:2971-2990 | MS.gene28463:CDS | 30.0% |
| !!! | CGTGTTTTTTTTTTTTGCAC+TGG | - | 15146:2599-2618 | MS.gene28463:intron | 30.0% |
| AAAAGCAATGAGACACAACA+TGG | + | 15146:2742-2761 | None:intergenic | 35.0% | |
| AGTCAGTATGTACTATGAAG+CGG | + | 15146:3125-3144 | None:intergenic | 35.0% | |
| ATTACAGGAGTTTCAACTAC+TGG | + | 15146:2508-2527 | None:intergenic | 35.0% | |
| TATGGGCAAGAATGTAAGAT+TGG | - | 15146:2702-2721 | MS.gene28463:intron | 35.0% | |
| TTTGAAGAGAAACTGGAATC+TGG | + | 15146:3057-3076 | None:intergenic | 35.0% | |
| ! | AAGAGTACACAAGTTAAGAC+TGG | - | 15146:3294-3313 | MS.gene28463:CDS | 35.0% |
| !! | GTAATTGATTTGACGCAGAT+TGG | - | 15146:2205-2224 | MS.gene28463:intron | 35.0% |
| !! | TCTATACCAGTTTGAGTTGA+AGG | + | 15146:2029-2048 | None:intergenic | 35.0% |
| AAATTCGCAGTCAATCCAGT+TGG | + | 15146:2241-2260 | None:intergenic | 40.0% | |
| AATCAAACACCTGTTAACGG+AGG | + | 15146:2328-2347 | None:intergenic | 40.0% | |
| ACACAAGTTAAGACTGGAGA+AGG | - | 15146:3300-3319 | MS.gene28463:CDS | 40.0% | |
| CACAAGTTAAGACTGGAGAA+GGG | - | 15146:3301-3320 | MS.gene28463:CDS | 40.0% | |
| GTGCAAGCACAATGTTTAAC+CGG | - | 15146:2880-2899 | MS.gene28463:intron | 40.0% | |
| ! | TGATACGTTTTTGTGCACTC+AGG | - | 15146:3258-3277 | MS.gene28463:intron | 40.0% |
| !! | GTGAGTAAATTTGCTGAACC+TGG | - | 15146:2457-2476 | MS.gene28463:intron | 40.0% |
| ACAACGAAAAGTGAGGGAAG+AGG | + | 15146:2057-2076 | None:intergenic | 45.0% | |
| ATTGGACAGAGTCATCCAAC+TGG | - | 15146:2223-2242 | MS.gene28463:intron | 45.0% | |
| CAACGTCCTTCAACTCAAAC+TGG | - | 15146:2020-2039 | MS.gene28463:intron | 45.0% | |
| CCATACAAAATGCAGGCACA+AGG | + | 15146:2918-2937 | None:intergenic | 45.0% | |
| CGAAAATGTCCTCCGTTAAC+AGG | - | 15146:2316-2335 | MS.gene28463:intron | 45.0% | |
| GGTGGAGGTTTATACTCCAA+CGG | + | 15146:2289-2308 | None:intergenic | 45.0% | |
| GTTGGAGTATAAACCTCCAC+CGG | - | 15146:2288-2307 | MS.gene28463:intron | 45.0% | |
| TGTTGAAGCTGTTTGAGCCT+AGG | - | 15146:2260-2279 | MS.gene28463:intron | 45.0% | |
| ! | AGCTTTGGTACGAGCTTGAA+CGG | + | 15146:1989-2008 | None:intergenic | 45.0% |
| ! | ATTTGCTGAACCTGGTGAAC+CGG | - | 15146:2465-2484 | MS.gene28463:intron | 45.0% |
| ! | CCTTGTGCCTGCATTTTGTA+TGG | - | 15146:2915-2934 | MS.gene28463:intron | 45.0% |
| ! | GGAGGACATTTTCGTTTCTC+CGG | + | 15146:2310-2329 | None:intergenic | 45.0% |
| ! | TTTTGCACTGGACATGTCTG+TGG | - | 15146:2611-2630 | MS.gene28463:intron | 45.0% |
| !! | CAGTTTGAGTTGAAGGACGT+TGG | + | 15146:2022-2041 | None:intergenic | 45.0% |
| CACAAGGAGTCAGAGATAGC+CGG | + | 15146:2902-2921 | None:intergenic | 50.0% | |
| GGAGAATATTCCGGTTCACC+AGG | + | 15146:2478-2497 | None:intergenic | 50.0% | |
| GGAGGTTTATACTCCAACGG+AGG | + | 15146:2286-2305 | None:intergenic | 50.0% | |
| GGAGTTTCAACTACTGGCAC+AGG | + | 15146:2502-2521 | None:intergenic | 50.0% | |
| GTACTATGAAGCGGCTACGT+CGG | + | 15146:3116-3135 | None:intergenic | 50.0% | |
| GTGCACTCAGGCAGAAAGAA+GGG | - | 15146:3270-3289 | MS.gene28463:CDS | 50.0% | |
| GTTTCAACTACTGGCACAGG+CGG | + | 15146:2499-2518 | None:intergenic | 50.0% | |
| TGTGCACTCAGGCAGAAAGA+AGG | - | 15146:3269-3288 | MS.gene28463:CDS | 50.0% | |
| TTATACTCCAACGGAGGCCT+AGG | + | 15146:2280-2299 | None:intergenic | 50.0% | |
| TTTGGTACGAGCTTGAACGG+CGG | + | 15146:1986-2005 | None:intergenic | 50.0% | |
| ! | CATTTTCGTTTCTCCGGTGG+AGG | + | 15146:2304-2323 | None:intergenic | 50.0% |
| ! | GGACATTTTCGTTTCTCCGG+TGG | + | 15146:2307-2326 | None:intergenic | 50.0% |
| GGCACAGGCGGAGAATATTC+CGG | + | 15146:2487-2506 | None:intergenic | 55.0% | |
| GTTGGCGCGATTCTGAGCTT+TGG | + | 15146:2004-2023 | None:intergenic | 55.0% | |
| TGCACTGGACATGTCTGTGG+CGG | - | 15146:2614-2633 | MS.gene28463:intron | 55.0% | |
| !!! | GCGTTTTGGTTGCGCAGAAG+AGG | + | 15146:1964-1983 | None:intergenic | 55.0% |
| GCACTGGACATGTCTGTGGC+GGG | - | 15146:2615-2634 | MS.gene28463:intron | 60.0% | |
| GTTTGAGCCTAGGCCTCCGT+TGG | - | 15146:2270-2289 | MS.gene28463:intron | 60.0% | |
| ! | GAGCTTGAACGGCGGCGTTT+TGG | + | 15146:1978-1997 | None:intergenic | 60.0% |
| GGGAGCTGCGAAAGCTGCTG+AGG | - | 15146:3321-3340 | MS.gene28463:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15146 | gene | 1952 | 3355 | 1952 | ID=MS.gene28463 |
| 15146 | mRNA | 1952 | 3355 | 1952 | ID=MS.gene28463.t1;Parent=MS.gene28463 |
| 15146 | exon | 3266 | 3355 | 3266 | ID=MS.gene28463.t1.exon1;Parent=MS.gene28463.t1 |
| 15146 | CDS | 3266 | 3355 | 3266 | ID=cds.MS.gene28463.t1;Parent=MS.gene28463.t1 |
| 15146 | exon | 2970 | 3084 | 2970 | ID=MS.gene28463.t1.exon2;Parent=MS.gene28463.t1 |
| 15146 | CDS | 2970 | 3084 | 2970 | ID=cds.MS.gene28463.t1;Parent=MS.gene28463.t1 |
| 15146 | exon | 2785 | 2864 | 2785 | ID=MS.gene28463.t1.exon3;Parent=MS.gene28463.t1 |
| 15146 | CDS | 2785 | 2864 | 2785 | ID=cds.MS.gene28463.t1;Parent=MS.gene28463.t1 |
| 15146 | exon | 1952 | 2027 | 1952 | ID=MS.gene28463.t1.exon4;Parent=MS.gene28463.t1 |
| 15146 | CDS | 1952 | 2027 | 1952 | ID=cds.MS.gene28463.t1;Parent=MS.gene28463.t1 |
| Gene Sequence |
| Protein sequence |
