Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28513.t1 | KEH20450.1 | 77 | 139 | 17 | 3 | 3 | 131 | 24 | 157 | 1.30E-46 | 196.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28513.t1 | Q9SVM0 | 38.0 | 100 | 31 | 1 | 22 | 90 | 115 | 214 | 1.3e-10 | 67.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28513.t1 | A0A072U3Q3 | 77.0 | 139 | 17 | 3 | 3 | 131 | 24 | 157 | 9.4e-47 | 196.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056173 | MS.gene28513 | 0.812125 | 4.96E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28513.t1 | MTR_8g078690 | 76.978 | 139 | 17 | 3 | 3 | 131 | 24 | 157 | 6.96e-67 | 202 |
| MS.gene28513.t1 | MTR_8g078495 | 51.587 | 126 | 20 | 2 | 6 | 90 | 99 | 224 | 1.35e-28 | 110 |
| MS.gene28513.t1 | MTR_4g124670 | 82.979 | 47 | 8 | 0 | 85 | 131 | 1624 | 1670 | 2.35e-22 | 93.2 |
| MS.gene28513.t1 | MTR_4g124670 | 82.979 | 47 | 8 | 0 | 85 | 131 | 1573 | 1619 | 2.37e-22 | 93.2 |
| MS.gene28513.t1 | MTR_4g124670 | 82.979 | 47 | 8 | 0 | 85 | 131 | 1624 | 1670 | 2.58e-22 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28513.t1 | AT3G50780 | 38.000 | 100 | 31 | 1 | 22 | 90 | 115 | 214 | 3.64e-14 | 69.3 |
| MS.gene28513.t1 | AT3G50780 | 38.000 | 100 | 31 | 1 | 22 | 90 | 115 | 214 | 3.64e-14 | 69.3 |
Find 28 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCTGTGAATGCATTTCT+TGG | 0.260452 | 8.3:+25658404 | MS.gene28513:CDS |
| CTAAAACTGTGTGGACTAAT+AGG | 0.336332 | 8.3:+25658483 | MS.gene28513:CDS |
| TACTTTAGATGTCATGGCTT+TGG | 0.348992 | 8.3:+25658460 | MS.gene28513:CDS |
| GTATCTTTAGCTATCGAGTT+TGG | 0.354321 | 8.3:+25657293 | MS.gene28513:CDS |
| TTGGTTGTGCAAGTTTCTCT+TGG | 0.369421 | 8.3:+25658423 | MS.gene28513:CDS |
| CATTTCTTCCACCAATGAAA+TGG | 0.402898 | 8.3:-25658541 | None:intergenic |
| TGAAAAGATTTCGAAGCAAT+CGG | 0.431158 | 8.3:+25657424 | MS.gene28513:intron |
| GCTATCGAGTTTGGTGAACC+TGG | 0.462242 | 8.3:+25657302 | MS.gene28513:CDS |
| GGTTCAGAAAGGTCTTTAGC+TGG | 0.463315 | 8.3:+25657248 | MS.gene28513:intron |
| TCCACCTACTTTAGATGTCA+TGG | 0.474844 | 8.3:+25658454 | MS.gene28513:CDS |
| AATGGCAAGTGCCATTTCAT+TGG | 0.475482 | 8.3:+25658530 | MS.gene28513:CDS |
| ATAGGTCTGCTAATTGTTCA+AGG | 0.481144 | 8.3:+25658501 | MS.gene28513:CDS |
| GGCAAGTGCCATTTCATTGG+TGG | 0.503466 | 8.3:+25658533 | MS.gene28513:CDS |
| GGAATATATGTTGAAGCTGT+TGG | 0.512707 | 8.3:+25657485 | MS.gene28513:CDS |
| AATTGTTCAAGGAACTGTAA+TGG | 0.518269 | 8.3:+25658512 | MS.gene28513:CDS |
| ACCTTCATATCACAAGTTCC+AGG | 0.526980 | 8.3:-25657320 | None:intergenic |
| TCGCATTATACAAATTCTCA+AGG | 0.531143 | 8.3:+25657559 | MS.gene28513:CDS |
| CAAGAAATGCATTCACAGCA+TGG | 0.578896 | 8.3:-25658403 | None:intergenic |
| ATCAGCGATCGTGAGGACGT+TGG | 0.581532 | 8.3:+25657464 | MS.gene28513:CDS |
| TCTTCAAATCAGCGATCGTG+AGG | 0.586073 | 8.3:+25657457 | MS.gene28513:CDS |
| ACCTGGAACTTGTGATATGA+AGG | 0.591247 | 8.3:+25657319 | MS.gene28513:CDS |
| GCCATGACATCTAAAGTAGG+TGG | 0.602118 | 8.3:-25658455 | None:intergenic |
| ACAGCGGCGAATGAAATACA+AGG | 0.610336 | 8.3:+25657532 | MS.gene28513:CDS |
| AAAGCCATGACATCTAAAGT+AGG | 0.625166 | 8.3:-25658458 | None:intergenic |
| TCTAAAGTAGGTGGAACATG+AGG | 0.650866 | 8.3:-25658446 | None:intergenic |
| TGGCTTTGGCTAAAACTGTG+TGG | 0.682013 | 8.3:+25658474 | MS.gene28513:CDS |
| TGTTGGTTTGATGTACTGCA+AGG | 0.702424 | 8.3:+25657502 | MS.gene28513:CDS |
| ACTGCAAGGAAATTAAACAG+CGG | 0.787596 | 8.3:+25657516 | MS.gene28513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAAATATCTTTTATATAGA+TGG | + | chr8.3:25657950-25657969 | MS.gene28513:intron | 15.0% |
| !!! | TATTTAGCAATTATTTGTTT+TGG | + | chr8.3:25658254-25658273 | MS.gene28513:intron | 15.0% |
| !!! | TTATAGATGCATATTTTTTT+GGG | + | chr8.3:25657855-25657874 | MS.gene28513:intron | 15.0% |
| !!! | TTTATAGATGCATATTTTTT+TGG | + | chr8.3:25657854-25657873 | MS.gene28513:intron | 15.0% |
| !! | ATGATCATGTAACAAAATTT+GGG | - | chr8.3:25658164-25658183 | None:intergenic | 20.0% |
| !! | TATAATGAAACCATATATCA+AGG | - | chr8.3:25657675-25657694 | None:intergenic | 20.0% |
| !! | TTCAAAAATATATACATAGC+TGG | + | chr8.3:25658059-25658078 | MS.gene28513:intron | 20.0% |
| !!! | CAGTTAATTGAATTTTTGAA+AGG | + | chr8.3:25658347-25658366 | MS.gene28513:intron | 20.0% |
| !!! | TTTTCTAAGCTATAGTTAAT+GGG | + | chr8.3:25658018-25658037 | MS.gene28513:intron | 20.0% |
| !!! | TTTTTCTAAGCTATAGTTAA+TGG | + | chr8.3:25658017-25658036 | MS.gene28513:intron | 20.0% |
| ! | ATATGAAGGTGGTTTAATTA+GGG | + | chr8.3:25657333-25657352 | MS.gene28513:intron | 25.0% |
| ! | CATGAAATAACTCAACAAAA+AGG | - | chr8.3:25657620-25657639 | None:intergenic | 25.0% |
| ! | CATGATCATGTAACAAAATT+TGG | - | chr8.3:25658165-25658184 | None:intergenic | 25.0% |
| ! | GCCAGAAAAATTTATCATAA+GGG | - | chr8.3:25657645-25657664 | None:intergenic | 25.0% |
| ! | GTATGCAACTCTTTATAAAA+TGG | - | chr8.3:25657914-25657933 | None:intergenic | 25.0% |
| ! | TCATGTAACAAAAATAACCA+AGG | - | chr8.3:25657884-25657903 | None:intergenic | 25.0% |
| ! | TGATCATGTAACAAAATTTG+GGG | - | chr8.3:25658163-25658182 | None:intergenic | 25.0% |
| ! | TGCCAGAAAAATTTATCATA+AGG | - | chr8.3:25657646-25657665 | None:intergenic | 25.0% |
| !! | AACTGCATATTCATTTGTTA+TGG | + | chr8.3:25657780-25657799 | MS.gene28513:intron | 25.0% |
| !! | ATTTTGTTACATGATCATGA+TGG | + | chr8.3:25658166-25658185 | MS.gene28513:intron | 25.0% |
| !! | TATTTTGTTCGATACTCAAT+TGG | + | chr8.3:25657985-25658004 | MS.gene28513:intron | 25.0% |
| !! | TCCCTTATGATAAATTTTTC+TGG | + | chr8.3:25657641-25657660 | MS.gene28513:intron | 25.0% |
| !!! | AATTGAATTTTTGAAAGGCT+TGG | + | chr8.3:25658352-25658371 | MS.gene28513:CDS | 25.0% |
| !!! | TATTGTCTTTTATTTGACAG+TGG | + | chr8.3:25658200-25658219 | MS.gene28513:intron | 25.0% |
| AACTACTGCTTCTAATGATT+TGG | + | chr8.3:25657723-25657742 | MS.gene28513:intron | 30.0% | |
| AATTGTTCAAGGAACTGTAA+TGG | + | chr8.3:25658512-25658531 | MS.gene28513:CDS | 30.0% | |
| ACAAATGAATATGCAGTTGT+TGG | - | chr8.3:25657778-25657797 | None:intergenic | 30.0% | |
| CAACAATGACAATGTAGTAT+TGG | + | chr8.3:25657227-25657246 | MS.gene28513:intron | 30.0% | |
| GAAACCATATATCAAGGTAA+CGG | - | chr8.3:25657669-25657688 | None:intergenic | 30.0% | |
| GATATGAAGGTGGTTTAATT+AGG | + | chr8.3:25657332-25657351 | MS.gene28513:intron | 30.0% | |
| GTTTCATTATACATGTACCT+CGG | + | chr8.3:25657684-25657703 | MS.gene28513:intron | 30.0% | |
| TCGCATTATACAAATTCTCA+AGG | + | chr8.3:25657559-25657578 | MS.gene28513:CDS | 30.0% | |
| TGAAAAGATTTCGAAGCAAT+CGG | + | chr8.3:25657424-25657443 | MS.gene28513:intron | 30.0% | |
| TGTAGATCACGATATAATCA+TGG | + | chr8.3:25658133-25658152 | MS.gene28513:intron | 30.0% | |
| TGTATAAGTGAGTGATAGAT+AGG | + | chr8.3:25657164-25657183 | MS.gene28513:intron | 30.0% | |
| ! | AAAAAAGTGTGTTGTCAAGT+TGG | + | chr8.3:25658224-25658243 | MS.gene28513:intron | 30.0% |
| ! | CCAAAATTAGCTTATCTTAC+TGG | - | chr8.3:25658317-25658336 | None:intergenic | 30.0% |
| ! | CCAGTAAGATAAGCTAATTT+TGG | + | chr8.3:25658314-25658333 | MS.gene28513:intron | 30.0% |
| ! | GCATATACAAGTTTGAGTTT+AGG | + | chr8.3:25657804-25657823 | MS.gene28513:intron | 30.0% |
| !! | AATGTAGTATTGGTTCAGAA+AGG | + | chr8.3:25657237-25657256 | MS.gene28513:intron | 30.0% |
| !!! | TAGATGCATATTTTTTTGGG+AGG | + | chr8.3:25657858-25657877 | MS.gene28513:intron | 30.0% |
| !!! | TTTGTTTTGGAAAAACATGC+TGG | + | chr8.3:25658267-25658286 | MS.gene28513:intron | 30.0% |
| ACTGCAAGGAAATTAAACAG+CGG | + | chr8.3:25657516-25657535 | MS.gene28513:CDS | 35.0% | |
| AGTATTGTACTCTGTGTCAT+TGG | - | chr8.3:25658108-25658127 | None:intergenic | 35.0% | |
| ATAGGTCTGCTAATTGTTCA+AGG | + | chr8.3:25658501-25658520 | MS.gene28513:CDS | 35.0% | |
| ATCACTAATCTAGATGATGC+AGG | - | chr8.3:25657197-25657216 | None:intergenic | 35.0% | |
| CATTTCTTCCACCAATGAAA+TGG | - | chr8.3:25658544-25658563 | None:intergenic | 35.0% | |
| GATGTTCTGATAGAGAATAG+TGG | + | chr8.3:25657392-25657411 | MS.gene28513:intron | 35.0% | |
| GCATACAAAGTAAACTTGTG+AGG | + | chr8.3:25657928-25657947 | MS.gene28513:intron | 35.0% | |
| GGAATATATGTTGAAGCTGT+TGG | + | chr8.3:25657485-25657504 | MS.gene28513:CDS | 35.0% | |
| GTATCTTTAGCTATCGAGTT+TGG | + | chr8.3:25657293-25657312 | MS.gene28513:CDS | 35.0% | |
| GTGGTTTAATTAGGGAAAGA+AGG | + | chr8.3:25657341-25657360 | MS.gene28513:intron | 35.0% | |
| TACTTTAGATGTCATGGCTT+TGG | + | chr8.3:25658460-25658479 | MS.gene28513:CDS | 35.0% | |
| ! | AAAGCCATGACATCTAAAGT+AGG | - | chr8.3:25658461-25658480 | None:intergenic | 35.0% |
| ! | CTAAAACTGTGTGGACTAAT+AGG | + | chr8.3:25658483-25658502 | MS.gene28513:CDS | 35.0% |
| !! | GAATTTTTGAAAGGCTTGGT+CGG | + | chr8.3:25658356-25658375 | MS.gene28513:CDS | 35.0% |
| !!! | AAGAAATGCTTTTCAGGTCA+TGG | + | chr8.3:25658556-25658575 | MS.gene28513:CDS | 35.0% |
| ACCTGGAACTTGTGATATGA+AGG | + | chr8.3:25657319-25657338 | MS.gene28513:CDS | 40.0% | |
| ACCTTCATATCACAAGTTCC+AGG | - | chr8.3:25657323-25657342 | None:intergenic | 40.0% | |
| CAAGAAATGCATTCACAGCA+TGG | - | chr8.3:25658406-25658425 | None:intergenic | 40.0% | |
| CATGCTGTGAATGCATTTCT+TGG | + | chr8.3:25658404-25658423 | MS.gene28513:CDS | 40.0% | |
| TAATAGCTATCTACAAGCCG+AGG | - | chr8.3:25657704-25657723 | None:intergenic | 40.0% | |
| TCCACCTACTTTAGATGTCA+TGG | + | chr8.3:25658454-25658473 | MS.gene28513:CDS | 40.0% | |
| TGGAACTTGTGATATGAAGG+TGG | + | chr8.3:25657322-25657341 | MS.gene28513:intron | 40.0% | |
| TTGGTTGTGCAAGTTTCTCT+TGG | + | chr8.3:25658423-25658442 | MS.gene28513:CDS | 40.0% | |
| ! | TCTAAAGTAGGTGGAACATG+AGG | - | chr8.3:25658449-25658468 | None:intergenic | 40.0% |
| ! | TGGTGGAAGAAATGCTTTTC+AGG | + | chr8.3:25658550-25658569 | MS.gene28513:CDS | 40.0% |
| !! | AATGGCAAGTGCCATTTCAT+TGG | + | chr8.3:25658530-25658549 | MS.gene28513:CDS | 40.0% |
| !! | GTGTCATTGGTAGTGACATA+CGG | - | chr8.3:25658095-25658114 | None:intergenic | 40.0% |
| !! | TGTCAAGTTGAGTGTGCATA+TGG | + | chr8.3:25657370-25657389 | MS.gene28513:intron | 40.0% |
| !! | TGTCATTGGTAGTGACATAC+GGG | - | chr8.3:25658094-25658113 | None:intergenic | 40.0% |
| !! | TGTTGGTTTGATGTACTGCA+AGG | + | chr8.3:25657502-25657521 | MS.gene28513:CDS | 40.0% |
| !!! | CATATTTTTTTGGGAGGCCT+TGG | + | chr8.3:25657864-25657883 | MS.gene28513:intron | 40.0% |
| ACAGCGGCGAATGAAATACA+AGG | + | chr8.3:25657532-25657551 | MS.gene28513:CDS | 45.0% | |
| ATGCAGTTGTTGGTCGCATT+TGG | - | chr8.3:25657768-25657787 | None:intergenic | 45.0% | |
| GGCACCGTTACCTTGATATA+TGG | + | chr8.3:25657662-25657681 | MS.gene28513:intron | 45.0% | |
| GGTTCAGAAAGGTCTTTAGC+TGG | + | chr8.3:25657248-25657267 | MS.gene28513:intron | 45.0% | |
| TCTTCAAATCAGCGATCGTG+AGG | + | chr8.3:25657457-25657476 | MS.gene28513:CDS | 45.0% | |
| ! | GCCATGACATCTAAAGTAGG+TGG | - | chr8.3:25658458-25658477 | None:intergenic | 45.0% |
| !! | AGTGTGTTGTCAAGTTGGCA+TGG | + | chr8.3:25658229-25658248 | MS.gene28513:intron | 45.0% |
| !! | TGGCTTTGGCTAAAACTGTG+TGG | + | chr8.3:25658474-25658493 | MS.gene28513:CDS | 45.0% |
| !! | TTTTTGAAAGGCTTGGTCGG+TGG | + | chr8.3:25658359-25658378 | MS.gene28513:CDS | 45.0% |
| ! | GCTATCGAGTTTGGTGAACC+TGG | + | chr8.3:25657302-25657321 | MS.gene28513:CDS | 50.0% |
| !! | GGCAAGTGCCATTTCATTGG+TGG | + | chr8.3:25658533-25658552 | MS.gene28513:CDS | 50.0% |
| ATCAGCGATCGTGAGGACGT+TGG | + | chr8.3:25657464-25657483 | MS.gene28513:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 25657152 | 25658586 | 25657152 | ID=MS.gene28513 |
| chr8.3 | mRNA | 25657152 | 25658586 | 25657152 | ID=MS.gene28513.t1;Parent=MS.gene28513 |
| chr8.3 | exon | 25657152 | 25657170 | 25657152 | ID=MS.gene28513.t1.exon1;Parent=MS.gene28513.t1 |
| chr8.3 | CDS | 25657152 | 25657170 | 25657152 | ID=cds.MS.gene28513.t1;Parent=MS.gene28513.t1 |
| chr8.3 | exon | 25657255 | 25657340 | 25657255 | ID=MS.gene28513.t1.exon2;Parent=MS.gene28513.t1 |
| chr8.3 | CDS | 25657255 | 25657340 | 25657255 | ID=cds.MS.gene28513.t1;Parent=MS.gene28513.t1 |
| chr8.3 | exon | 25657431 | 25657580 | 25657431 | ID=MS.gene28513.t1.exon3;Parent=MS.gene28513.t1 |
| chr8.3 | CDS | 25657431 | 25657580 | 25657431 | ID=cds.MS.gene28513.t1;Parent=MS.gene28513.t1 |
| chr8.3 | exon | 25658350 | 25658586 | 25658350 | ID=MS.gene28513.t1.exon4;Parent=MS.gene28513.t1 |
| chr8.3 | CDS | 25658350 | 25658586 | 25658350 | ID=cds.MS.gene28513.t1;Parent=MS.gene28513.t1 |
| Gene Sequence |
| Protein sequence |
