Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28521.t1 | XP_028762943.1 | 70.1 | 368 | 84 | 2 | 1 | 362 | 1 | 348 | 6.80E-112 | 414.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28521.t1 | A0A1U8KII2 | 63.2 | 348 | 116 | 1 | 1 | 348 | 1 | 336 | 9.3e-103 | 383.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059272 | MS.gene28521 | 0.867344 | 1.47E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28521.t1 | MTR_7g062720 | 65.909 | 132 | 45 | 0 | 1 | 132 | 1 | 132 | 1.72e-59 | 189 |
| MS.gene28521.t1 | MTR_5g061150 | 62.992 | 127 | 47 | 0 | 20 | 146 | 10 | 136 | 5.61e-48 | 159 |
| MS.gene28521.t1 | MTR_4g037355 | 55.714 | 140 | 37 | 4 | 151 | 289 | 1 | 116 | 4.73e-41 | 141 |
| MS.gene28521.t1 | MTR_4g112730 | 69.863 | 73 | 20 | 1 | 138 | 210 | 1 | 71 | 9.41e-27 | 102 |
| MS.gene28521.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 282 | 362 | 5 | 80 | 1.09e-22 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 66 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCTCTTAATATTTC+TGG | 0.080746 | 8.4:+27940661 | MS.gene28521:CDS |
| TTCGGCATTTAAATGAATTT+TGG | 0.166319 | 8.4:-27940713 | None:intergenic |
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 8.4:+27941562 | MS.gene28521:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 8.4:+27941554 | MS.gene28521:CDS |
| TATGGAAGGGGACGTGGTTT+TGG | 0.259444 | 8.4:+27941327 | MS.gene28521:CDS |
| TGCGTTGCTACATTCACTTC+TGG | 0.264941 | 8.4:-27941225 | None:intergenic |
| TAGAAGATCCAAAAGATCTT+TGG | 0.269782 | 8.4:+27940851 | MS.gene28521:CDS |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 8.4:+27940675 | MS.gene28521:CDS |
| ATGGTTGTATTTGCGTTTAC+AGG | 0.299146 | 8.4:+27940936 | MS.gene28521:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 8.4:-27941716 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 8.4:-27941531 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 8.4:+27941614 | MS.gene28521:CDS |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 8.4:+27940680 | MS.gene28521:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.361577 | 8.4:+27940681 | MS.gene28521:CDS |
| CGTGGACGTGGTCGTGGTTA+TGG | 0.362984 | 8.4:+27941309 | MS.gene28521:CDS |
| TTTGGGAAAATTTAAAAGAA+AGG | 0.385472 | 8.4:+27940869 | MS.gene28521:CDS |
| AACCATTCGTTACGAGCATT+AGG | 0.390171 | 8.4:-27940919 | None:intergenic |
| AGAAGATCCAAAAGATCTTT+GGG | 0.393236 | 8.4:+27940852 | MS.gene28521:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.394855 | 8.4:+27941606 | MS.gene28521:CDS |
| CGTGGTCGTGGCCATGGTTA+TGG | 0.406960 | 8.4:+27941273 | MS.gene28521:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 8.4:+27941483 | MS.gene28521:CDS |
| CGTGGTTATGGAAGGGGACG+TGG | 0.429618 | 8.4:+27941321 | MS.gene28521:CDS |
| GCGTTGCTACATTCACTTCT+GGG | 0.431019 | 8.4:-27941224 | None:intergenic |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 8.4:+27940804 | MS.gene28521:CDS |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 8.4:+27940765 | MS.gene28521:CDS |
| CAAACTCGTGGTCGTGGTCG+TGG | 0.459890 | 8.4:+27941261 | MS.gene28521:CDS |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 8.4:-27941644 | None:intergenic |
| AATGGTATCTCCATGCCCTT+CGG | 0.470113 | 8.4:-27940731 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 8.4:+27941432 | MS.gene28521:CDS |
| CGTGGTCGTGGTCGTGGCCA+TGG | 0.475975 | 8.4:+27941267 | MS.gene28521:CDS |
| ATTGACACTGTGTGGAGAAA+AGG | 0.483584 | 8.4:+27941011 | MS.gene28521:CDS |
| GTAGCAACGCATGATCACTA+TGG | 0.484635 | 8.4:+27941237 | MS.gene28521:CDS |
| GCAGCAACAATACCGCGAAA+AGG | 0.486558 | 8.4:+27941095 | MS.gene28521:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 8.4:+27941563 | MS.gene28521:CDS |
| GCTACATTCACTTCTGGGAA+TGG | 0.488554 | 8.4:-27941219 | None:intergenic |
| TACATTAGATGCATGAAAAG+TGG | 0.490606 | 8.4:-27941067 | None:intergenic |
| CAGCAACAATACCGCGAAAA+GGG | 0.491478 | 8.4:+27941096 | MS.gene28521:CDS |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 8.4:+27940721 | MS.gene28521:CDS |
| GACGTGGTCGTGGTTATGGA+AGG | 0.501950 | 8.4:+27941313 | MS.gene28521:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 8.4:+27941490 | MS.gene28521:CDS |
| TATGGACAAACTCGTGGTCG+TGG | 0.513531 | 8.4:+27941255 | MS.gene28521:CDS |
| CATGGAGATACCATTAAAGA+AGG | 0.521442 | 8.4:+27940739 | MS.gene28521:CDS |
| CGTGGACGTGGACGTGGTCG+TGG | 0.524981 | 8.4:+27941303 | MS.gene28521:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 8.4:+27941698 | MS.gene28521:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.537172 | 8.4:+27940715 | MS.gene28521:CDS |
| CATGGTTATGGTCGTGGACG+TGG | 0.538890 | 8.4:+27941285 | MS.gene28521:CDS |
| CGTGGCCATGGTTATGGTCG+TGG | 0.540024 | 8.4:+27941279 | MS.gene28521:CDS |
| CGTGGACGTGGACGTGGACG+TGG | 0.546682 | 8.4:+27941297 | MS.gene28521:CDS |
| CGTGGTCGTGGTTATGGAAG+GGG | 0.551769 | 8.4:+27941315 | MS.gene28521:CDS |
| TATGGTCGTGGACGTGGACG+TGG | 0.556253 | 8.4:+27941291 | MS.gene28521:CDS |
| TTCCTAATGCTCGTAACGAA+TGG | 0.575736 | 8.4:+27940917 | MS.gene28521:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 8.4:+27940771 | MS.gene28521:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 8.4:+27941474 | MS.gene28521:CDS |
| ATTCGTTACGAGCATTAGGA+AGG | 0.589689 | 8.4:-27940915 | None:intergenic |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 8.4:-27940804 | None:intergenic |
| ACGTGGTCGTGGTTATGGAA+GGG | 0.598966 | 8.4:+27941314 | MS.gene28521:CDS |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 8.4:+27941477 | MS.gene28521:CDS |
| AATCACACGACTCGTCCCAC+TGG | 0.600994 | 8.4:+27941189 | MS.gene28521:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 8.4:-27941643 | None:intergenic |
| AAATTCATTTAAATGCCGAA+GGG | 0.631745 | 8.4:+27940716 | MS.gene28521:CDS |
| CACGTCCACGACCATAACCA+TGG | 0.649591 | 8.4:-27941284 | None:intergenic |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 8.4:-27940794 | None:intergenic |
| ACTTCTCTATTGACACTGTG+TGG | 0.668278 | 8.4:+27941003 | MS.gene28521:CDS |
| TGGGAATGGAGCACTACCAG+TGG | 0.672955 | 8.4:-27941205 | None:intergenic |
| GGGAATGGAGCACTACCAGT+GGG | 0.676344 | 8.4:-27941204 | None:intergenic |
| GATCACTATGGACAAACTCG+TGG | 0.716183 | 8.4:+27941249 | MS.gene28521:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | + | chr8.4:27941432-27941451 | MS.gene28521:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | + | chr8.4:27941563-27941582 | MS.gene28521:CDS | 20.0% |
| !! | TTTGGGAAAATTTAAAAGAA+AGG | + | chr8.4:27940869-27940888 | MS.gene28521:CDS | 20.0% |
| !!! | TATTTCTGGAAATAATTATC+TGG | + | chr8.4:27940675-27940694 | MS.gene28521:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | + | chr8.4:27940715-27940734 | MS.gene28521:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | + | chr8.4:27940716-27940735 | MS.gene28521:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | + | chr8.4:27941562-27941581 | MS.gene28521:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | + | chr8.4:27941606-27941625 | MS.gene28521:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | + | chr8.4:27941614-27941633 | MS.gene28521:CDS | 25.0% |
| !! | AAATTTTCCCAAAGATCTTT+TGG | - | chr8.4:27940862-27940881 | None:intergenic | 25.0% |
| !! | ATCATTTTCACATGCAAAAT+GGG | - | chr8.4:27941595-27941614 | None:intergenic | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | - | chr8.4:27941534-27941553 | None:intergenic | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | - | chr8.4:27941422-27941441 | None:intergenic | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | - | chr8.4:27940716-27940735 | None:intergenic | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | - | chr8.4:27940819-27940838 | None:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | - | chr8.4:27941398-27941417 | None:intergenic | 25.0% |
| !!! | TAAATGAATTTTGGCATCAA+GGG | - | chr8.4:27940707-27940726 | None:intergenic | 25.0% |
| !!! | TGTTTTGTTTCCTTCTTTAA+TGG | - | chr8.4:27940752-27940771 | None:intergenic | 25.0% |
| !!! | TTAAATGAATTTTGGCATCA+AGG | - | chr8.4:27940708-27940727 | None:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | + | chr8.4:27940852-27940871 | MS.gene28521:CDS | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | + | chr8.4:27940765-27940784 | MS.gene28521:CDS | 30.0% | |
| CATTAGAGATGAAAATATCC+AGG | + | chr8.4:27941698-27941717 | MS.gene28521:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | + | chr8.4:27941554-27941573 | MS.gene28521:CDS | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | - | chr8.4:27941070-27941089 | None:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | + | chr8.4:27940851-27940870 | MS.gene28521:CDS | 30.0% | |
| ! | AAGATCTTTTGGATCTTCTA+TGG | - | chr8.4:27940851-27940870 | None:intergenic | 30.0% |
| ! | AGATGATCAATCTTTTCATC+AGG | - | chr8.4:27941678-27941697 | None:intergenic | 30.0% |
| ! | CATCATTTTCACATGCAAAA+TGG | - | chr8.4:27941596-27941615 | None:intergenic | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | + | chr8.4:27940681-27940700 | MS.gene28521:CDS | 30.0% |
| ! | TTTGAGGCTCTTAATATTTC+TGG | + | chr8.4:27940661-27940680 | MS.gene28521:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | - | chr8.4:27940816-27940835 | None:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | + | chr8.4:27941348-27941367 | MS.gene28521:CDS | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | - | chr8.4:27941557-27941576 | None:intergenic | 30.0% |
| AACTGAACAACAAAAGGCTA+AGG | + | chr8.4:27940771-27940790 | MS.gene28521:CDS | 35.0% | |
| ATGGTTGTATTTGCGTTTAC+AGG | + | chr8.4:27940936-27940955 | MS.gene28521:CDS | 35.0% | |
| CATGGAGATACCATTAAAGA+AGG | + | chr8.4:27940739-27940758 | MS.gene28521:CDS | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | + | chr8.4:27940680-27940699 | MS.gene28521:CDS | 35.0% | |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | - | chr8.4:27940648-27940667 | None:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | + | chr8.4:27940645-27940664 | MS.gene28521:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | - | chr8.4:27940846-27940865 | None:intergenic | 35.0% |
| !!! | ACCTTTTAAAACCCTTTTCG+CGG | - | chr8.4:27941110-27941129 | None:intergenic | 35.0% |
| AACCATTCGTTACGAGCATT+AGG | - | chr8.4:27940922-27940941 | None:intergenic | 40.0% | |
| ACTTCTCTATTGACACTGTG+TGG | + | chr8.4:27941003-27941022 | MS.gene28521:CDS | 40.0% | |
| AGAAATCAGCAGCATCTAAG+TGG | - | chr8.4:27941647-27941666 | None:intergenic | 40.0% | |
| ATTCGTTACGAGCATTAGGA+AGG | - | chr8.4:27940918-27940937 | None:intergenic | 40.0% | |
| ATTGACACTGTGTGGAGAAA+AGG | + | chr8.4:27941011-27941030 | MS.gene28521:CDS | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | - | chr8.4:27941646-27941665 | None:intergenic | 40.0% | |
| TTCCTAATGCTCGTAACGAA+TGG | + | chr8.4:27940917-27940936 | MS.gene28521:CDS | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | - | chr8.4:27940900-27940919 | None:intergenic | 40.0% |
| !! | AGTATTTGCTATCGTTGTGG+AGG | + | chr8.4:27941477-27941496 | MS.gene28521:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | + | chr8.4:27941474-27941493 | MS.gene28521:CDS | 40.0% |
| !!! | ACCGCGAAAAGGGTTTTAAA+AGG | + | chr8.4:27941106-27941125 | MS.gene28521:CDS | 40.0% |
| AATGGTATCTCCATGCCCTT+CGG | - | chr8.4:27940734-27940753 | None:intergenic | 45.0% | |
| CAGCAACAATACCGCGAAAA+GGG | + | chr8.4:27941096-27941115 | MS.gene28521:CDS | 45.0% | |
| CATTTAAATGCCGAAGGGCA+TGG | + | chr8.4:27940721-27940740 | MS.gene28521:CDS | 45.0% | |
| GATCACTATGGACAAACTCG+TGG | + | chr8.4:27941249-27941268 | MS.gene28521:CDS | 45.0% | |
| GCGTTGCTACATTCACTTCT+GGG | - | chr8.4:27941227-27941246 | None:intergenic | 45.0% | |
| GTAGCAACGCATGATCACTA+TGG | + | chr8.4:27941237-27941256 | MS.gene28521:CDS | 45.0% | |
| GTTGTGGAGGAAAAGGTCAT+TGG | + | chr8.4:27941490-27941509 | MS.gene28521:CDS | 45.0% | |
| TGCGTTGCTACATTCACTTC+TGG | - | chr8.4:27941228-27941247 | None:intergenic | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | + | chr8.4:27941483-27941502 | MS.gene28521:CDS | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | - | chr8.4:27941222-27941241 | None:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | - | chr8.4:27940797-27940816 | None:intergenic | 45.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | - | chr8.4:27941523-27941542 | None:intergenic | 45.0% |
| ACGTGGTCGTGGTTATGGAA+GGG | + | chr8.4:27941314-27941333 | MS.gene28521:CDS | 50.0% | |
| CCTTCGTCACCACCTTCATA+AGG | + | chr8.4:27940804-27940823 | MS.gene28521:CDS | 50.0% | |
| GCAGCAACAATACCGCGAAA+AGG | + | chr8.4:27941095-27941114 | MS.gene28521:CDS | 50.0% | |
| TATGGACAAACTCGTGGTCG+TGG | + | chr8.4:27941255-27941274 | MS.gene28521:CDS | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | - | chr8.4:27940807-27940826 | None:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | + | chr8.4:27941327-27941346 | MS.gene28521:CDS | 50.0% |
| AATCACACGACTCGTCCCAC+TGG | + | chr8.4:27941189-27941208 | MS.gene28521:CDS | 55.0% | |
| CACGTCCACGACCATAACCA+TGG | - | chr8.4:27941287-27941306 | None:intergenic | 55.0% | |
| CATGGTTATGGTCGTGGACG+TGG | + | chr8.4:27941285-27941304 | MS.gene28521:CDS | 55.0% | |
| CGTGGTCGTGGTTATGGAAG+GGG | + | chr8.4:27941315-27941334 | MS.gene28521:CDS | 55.0% | |
| GACGTGGTCGTGGTTATGGA+AGG | + | chr8.4:27941313-27941332 | MS.gene28521:CDS | 55.0% | |
| ! | GGGAATGGAGCACTACCAGT+GGG | - | chr8.4:27941207-27941226 | None:intergenic | 55.0% |
| ! | TGGGAATGGAGCACTACCAG+TGG | - | chr8.4:27941208-27941227 | None:intergenic | 55.0% |
| CAAACTCGTGGTCGTGGTCG+TGG | + | chr8.4:27941261-27941280 | MS.gene28521:CDS | 60.0% | |
| CGTGGACGTGGTCGTGGTTA+TGG | + | chr8.4:27941309-27941328 | MS.gene28521:CDS | 60.0% | |
| CGTGGCCATGGTTATGGTCG+TGG | + | chr8.4:27941279-27941298 | MS.gene28521:CDS | 60.0% | |
| CGTGGTCGTGGCCATGGTTA+TGG | + | chr8.4:27941273-27941292 | MS.gene28521:CDS | 60.0% | |
| CGTGGTTATGGAAGGGGACG+TGG | + | chr8.4:27941321-27941340 | MS.gene28521:CDS | 60.0% | |
| TATGGTCGTGGACGTGGACG+TGG | + | chr8.4:27941291-27941310 | MS.gene28521:CDS | 60.0% | |
| CGTGGACGTGGACGTGGACG+TGG | + | chr8.4:27941297-27941316 | MS.gene28521:CDS | 70.0% | |
| CGTGGACGTGGACGTGGTCG+TGG | + | chr8.4:27941303-27941322 | MS.gene28521:CDS | 70.0% | |
| CGTGGTCGTGGTCGTGGCCA+TGG | + | chr8.4:27941267-27941286 | MS.gene28521:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 27940637 | 27941725 | 27940637 | ID=MS.gene28521 |
| chr8.4 | mRNA | 27940637 | 27941725 | 27940637 | ID=MS.gene28521.t1;Parent=MS.gene28521 |
| chr8.4 | exon | 27940637 | 27941725 | 27940637 | ID=MS.gene28521.t1.exon1;Parent=MS.gene28521.t1 |
| chr8.4 | CDS | 27940637 | 27941725 | 27940637 | ID=cds.MS.gene28521.t1;Parent=MS.gene28521.t1 |
| Gene Sequence |
| Protein sequence |
