Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28849.t1 | AES82246.2 | 82.4 | 267 | 11 | 2 | 1 | 252 | 1 | 246 | 1.30E-109 | 406 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28849.t1 | Q94K41 | 58.1 | 258 | 90 | 2 | 1 | 243 | 1 | 255 | 8.5e-78 | 291.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28849.t1 | G7KTR6 | 82.4 | 267 | 11 | 2 | 1 | 252 | 1 | 246 | 9.3e-110 | 406.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050430 | MS.gene28849 | 0.8277 | 1.35E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28849.t1 | MTR_7g110160 | 82.397 | 267 | 11 | 2 | 1 | 252 | 1 | 246 | 9.92e-152 | 422 |
| MS.gene28849.t1 | MTR_7g110130 | 82.239 | 259 | 29 | 2 | 1 | 244 | 1 | 257 | 1.63e-150 | 420 |
| MS.gene28849.t1 | MTR_2g090165 | 76.493 | 268 | 42 | 3 | 1 | 250 | 1 | 265 | 8.93e-144 | 403 |
| MS.gene28849.t1 | MTR_7g110140 | 65.116 | 258 | 72 | 2 | 1 | 243 | 1 | 255 | 5.03e-115 | 330 |
| MS.gene28849.t1 | MTR_6g035395 | 56.705 | 261 | 82 | 3 | 3 | 248 | 2 | 246 | 3.04e-96 | 282 |
| MS.gene28849.t1 | MTR_6g023590 | 50.000 | 252 | 108 | 3 | 5 | 240 | 12 | 261 | 3.07e-81 | 244 |
| MS.gene28849.t1 | MTR_6g023950 | 49.804 | 255 | 110 | 3 | 5 | 243 | 12 | 264 | 7.54e-79 | 238 |
| MS.gene28849.t1 | MTR_6g023610 | 49.206 | 252 | 110 | 3 | 5 | 240 | 12 | 261 | 4.06e-78 | 237 |
| MS.gene28849.t1 | MTR_6g023910 | 48.235 | 255 | 114 | 3 | 2 | 240 | 9 | 261 | 1.49e-76 | 233 |
| MS.gene28849.t1 | MTR_6g023990 | 47.451 | 255 | 116 | 3 | 2 | 240 | 10 | 262 | 4.59e-76 | 231 |
| MS.gene28849.t1 | MTR_7g071680 | 47.426 | 272 | 109 | 3 | 2 | 241 | 25 | 294 | 3.86e-75 | 230 |
| MS.gene28849.t1 | MTR_6g023600 | 48.016 | 252 | 113 | 3 | 5 | 240 | 12 | 261 | 1.74e-74 | 228 |
| MS.gene28849.t1 | MTR_4g052320 | 48.221 | 253 | 115 | 3 | 5 | 244 | 7 | 256 | 3.60e-74 | 227 |
| MS.gene28849.t1 | MTR_4g052300 | 47.266 | 256 | 120 | 3 | 2 | 245 | 4 | 256 | 1.71e-73 | 225 |
| MS.gene28849.t1 | MTR_4g052350 | 48.819 | 254 | 115 | 3 | 4 | 245 | 12 | 262 | 2.40e-73 | 225 |
| MS.gene28849.t1 | MTR_4g052340 | 47.244 | 254 | 119 | 3 | 4 | 245 | 12 | 262 | 7.97e-72 | 221 |
| MS.gene28849.t1 | MTR_6g023600 | 47.619 | 252 | 108 | 4 | 5 | 240 | 12 | 255 | 6.83e-71 | 218 |
| MS.gene28849.t1 | MTR_7g105460 | 45.882 | 255 | 120 | 3 | 5 | 244 | 12 | 263 | 2.96e-68 | 212 |
| MS.gene28849.t1 | MTR_7g083870 | 44.487 | 263 | 123 | 5 | 4 | 246 | 33 | 292 | 2.08e-67 | 211 |
| MS.gene28849.t1 | MTR_7g107670 | 43.411 | 258 | 122 | 4 | 4 | 241 | 31 | 284 | 1.32e-66 | 208 |
| MS.gene28849.t1 | MTR_4g078630 | 43.820 | 267 | 121 | 5 | 1 | 244 | 14 | 274 | 1.20e-61 | 195 |
| MS.gene28849.t1 | MTR_7g105580 | 42.188 | 256 | 112 | 3 | 4 | 244 | 6 | 240 | 2.17e-61 | 194 |
| MS.gene28849.t1 | MTR_3g020670 | 42.697 | 267 | 128 | 4 | 4 | 248 | 14 | 277 | 1.14e-57 | 185 |
| MS.gene28849.t1 | MTR_7g105590 | 48.691 | 191 | 95 | 2 | 4 | 194 | 6 | 193 | 1.63e-55 | 177 |
| MS.gene28849.t1 | MTR_3g113870 | 44.865 | 185 | 98 | 3 | 14 | 198 | 29 | 209 | 1.80e-46 | 155 |
| MS.gene28849.t1 | MTR_7g105570 | 35.496 | 262 | 96 | 6 | 2 | 244 | 13 | 220 | 6.14e-40 | 138 |
| MS.gene28849.t1 | MTR_4g084800 | 48.571 | 140 | 70 | 1 | 4 | 143 | 11 | 148 | 2.27e-39 | 134 |
| MS.gene28849.t1 | MTR_6g023940 | 35.814 | 215 | 59 | 5 | 2 | 208 | 9 | 152 | 5.62e-29 | 107 |
| MS.gene28849.t1 | MTR_4g058840 | 30.657 | 274 | 148 | 9 | 1 | 242 | 1 | 264 | 8.80e-24 | 97.1 |
| MS.gene28849.t1 | MTR_4g058820 | 30.657 | 274 | 148 | 9 | 1 | 242 | 1 | 264 | 8.80e-24 | 97.1 |
| MS.gene28849.t1 | MTR_3g085740 | 26.275 | 255 | 165 | 8 | 4 | 242 | 73 | 320 | 4.81e-21 | 90.5 |
| MS.gene28849.t1 | MTR_4g064887 | 28.077 | 260 | 159 | 7 | 2 | 242 | 11 | 261 | 3.19e-20 | 87.4 |
| MS.gene28849.t1 | MTR_4g058810 | 29.562 | 274 | 151 | 9 | 1 | 242 | 1 | 264 | 4.35e-20 | 87.0 |
| MS.gene28849.t1 | MTR_1g051165 | 29.167 | 264 | 159 | 7 | 4 | 242 | 12 | 272 | 2.52e-19 | 85.1 |
| MS.gene28849.t1 | MTR_7g113650 | 26.718 | 262 | 162 | 8 | 5 | 244 | 39 | 292 | 8.80e-18 | 80.9 |
| MS.gene28849.t1 | MTR_1g082760 | 27.481 | 262 | 160 | 8 | 5 | 244 | 39 | 292 | 1.47e-17 | 80.5 |
| MS.gene28849.t1 | MTR_4g132850 | 28.458 | 253 | 158 | 6 | 3 | 240 | 4 | 248 | 3.15e-17 | 79.0 |
| MS.gene28849.t1 | MTR_4g131740 | 26.538 | 260 | 163 | 9 | 5 | 242 | 39 | 292 | 9.58e-17 | 78.2 |
| MS.gene28849.t1 | MTR_3g068175 | 29.070 | 258 | 161 | 7 | 1 | 242 | 12 | 263 | 2.90e-16 | 76.6 |
| MS.gene28849.t1 | MTR_4g064887 | 29.703 | 202 | 128 | 5 | 2 | 197 | 11 | 204 | 3.05e-16 | 75.5 |
| MS.gene28849.t1 | MTR_3g068070 | 27.734 | 256 | 165 | 7 | 2 | 242 | 11 | 261 | 7.33e-16 | 75.5 |
| MS.gene28849.t1 | MTR_7g105560 | 62.500 | 56 | 21 | 0 | 4 | 59 | 11 | 66 | 7.15e-15 | 70.5 |
| MS.gene28849.t1 | MTR_4g086400 | 27.984 | 243 | 140 | 6 | 8 | 226 | 6 | 237 | 1.06e-14 | 73.6 |
| MS.gene28849.t1 | MTR_3g068155 | 28.125 | 256 | 162 | 8 | 3 | 242 | 16 | 265 | 1.23e-14 | 72.0 |
| MS.gene28849.t1 | MTR_4g086410 | 28.807 | 243 | 138 | 6 | 8 | 226 | 6 | 237 | 1.49e-14 | 73.2 |
| MS.gene28849.t1 | MTR_7g094510 | 27.586 | 261 | 161 | 8 | 1 | 240 | 1 | 254 | 1.54e-14 | 71.6 |
| MS.gene28849.t1 | MTR_3g068085 | 27.381 | 252 | 163 | 7 | 6 | 242 | 15 | 261 | 3.04e-14 | 70.9 |
| MS.gene28849.t1 | MTR_3g068175 | 28.626 | 262 | 161 | 8 | 1 | 242 | 12 | 267 | 3.90e-14 | 70.5 |
| MS.gene28849.t1 | MTR_7g063320 | 28.839 | 267 | 152 | 10 | 2 | 242 | 11 | 265 | 3.64e-13 | 67.8 |
| MS.gene28849.t1 | MTR_7g094520 | 28.854 | 253 | 158 | 8 | 6 | 240 | 11 | 259 | 1.04e-12 | 66.2 |
| MS.gene28849.t1 | MTR_7g063360 | 26.718 | 262 | 164 | 8 | 2 | 242 | 12 | 266 | 2.24e-12 | 65.5 |
| MS.gene28849.t1 | MTR_7g063270 | 27.203 | 261 | 159 | 8 | 3 | 241 | 13 | 264 | 3.31e-12 | 65.1 |
| MS.gene28849.t1 | MTR_4g097510 | 26.042 | 192 | 135 | 4 | 7 | 197 | 3 | 188 | 1.22e-11 | 63.5 |
| MS.gene28849.t1 | MTR_7g063390 | 26.515 | 264 | 160 | 7 | 3 | 242 | 13 | 266 | 2.11e-11 | 62.8 |
| MS.gene28849.t1 | MTR_7g063260 | 26.437 | 261 | 162 | 7 | 3 | 241 | 13 | 265 | 3.73e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28849.t1 | AT2G47140 | 58.140 | 258 | 90 | 2 | 1 | 243 | 1 | 255 | 5.33e-104 | 302 |
| MS.gene28849.t1 | AT3G29250 | 55.212 | 259 | 98 | 3 | 1 | 244 | 1 | 256 | 1.87e-93 | 276 |
| MS.gene28849.t1 | AT3G29250 | 55.426 | 258 | 97 | 3 | 1 | 243 | 37 | 291 | 4.64e-93 | 276 |
| MS.gene28849.t1 | AT2G47130 | 53.101 | 258 | 104 | 2 | 1 | 244 | 1 | 255 | 4.82e-93 | 275 |
| MS.gene28849.t1 | AT3G29260 | 54.651 | 258 | 100 | 3 | 1 | 244 | 1 | 255 | 1.59e-92 | 273 |
| MS.gene28849.t1 | AT3G29250 | 55.078 | 256 | 97 | 3 | 3 | 243 | 41 | 293 | 5.28e-92 | 273 |
| MS.gene28849.t1 | AT2G47120 | 52.490 | 261 | 106 | 3 | 1 | 247 | 1 | 257 | 1.42e-90 | 268 |
| MS.gene28849.t1 | AT2G47120 | 52.529 | 257 | 104 | 3 | 5 | 247 | 6 | 258 | 1.16e-89 | 266 |
| MS.gene28849.t1 | AT3G51680 | 46.840 | 269 | 115 | 2 | 1 | 241 | 27 | 295 | 2.15e-73 | 226 |
| MS.gene28849.t1 | AT3G26760 | 40.076 | 262 | 132 | 4 | 2 | 241 | 32 | 290 | 4.70e-62 | 197 |
| MS.gene28849.t1 | AT4G03140 | 43.462 | 260 | 123 | 4 | 4 | 243 | 76 | 331 | 4.73e-62 | 198 |
| MS.gene28849.t1 | AT1G52340 | 41.852 | 270 | 129 | 4 | 4 | 248 | 16 | 282 | 5.44e-59 | 189 |
| MS.gene28849.t1 | AT3G26770 | 41.473 | 258 | 128 | 4 | 4 | 241 | 39 | 293 | 9.76e-59 | 189 |
| MS.gene28849.t1 | AT3G42960 | 45.076 | 264 | 115 | 5 | 2 | 241 | 4 | 261 | 3.74e-56 | 181 |
| MS.gene28849.t1 | AT2G47150 | 37.083 | 240 | 108 | 5 | 6 | 244 | 1 | 198 | 2.94e-39 | 135 |
| MS.gene28849.t1 | AT2G29340 | 29.297 | 256 | 159 | 6 | 3 | 242 | 4 | 253 | 2.21e-21 | 90.5 |
| MS.gene28849.t1 | AT2G29340 | 29.297 | 256 | 159 | 6 | 3 | 242 | 4 | 253 | 6.31e-21 | 90.1 |
| MS.gene28849.t1 | AT3G55290 | 28.794 | 257 | 160 | 6 | 6 | 242 | 17 | 270 | 1.80e-20 | 88.2 |
| MS.gene28849.t1 | AT3G55290 | 28.794 | 257 | 160 | 6 | 6 | 242 | 18 | 271 | 1.81e-20 | 88.2 |
| MS.gene28849.t1 | AT2G29310 | 28.731 | 268 | 163 | 7 | 3 | 251 | 4 | 262 | 3.14e-20 | 87.4 |
| MS.gene28849.t1 | AT3G55310 | 29.730 | 259 | 155 | 8 | 6 | 242 | 17 | 270 | 4.11e-20 | 87.4 |
| MS.gene28849.t1 | AT2G29290 | 31.086 | 267 | 140 | 8 | 3 | 242 | 4 | 253 | 1.11e-19 | 85.9 |
| MS.gene28849.t1 | AT1G07450 | 29.183 | 257 | 161 | 6 | 1 | 242 | 3 | 253 | 1.58e-19 | 85.5 |
| MS.gene28849.t1 | AT1G62610 | 28.295 | 258 | 161 | 6 | 6 | 242 | 15 | 269 | 1.88e-19 | 85.5 |
| MS.gene28849.t1 | AT1G62610 | 28.295 | 258 | 161 | 6 | 6 | 242 | 13 | 267 | 2.06e-19 | 85.5 |
| MS.gene28849.t1 | AT1G62610 | 28.295 | 258 | 161 | 6 | 6 | 242 | 19 | 273 | 2.23e-19 | 85.5 |
| MS.gene28849.t1 | AT1G62610 | 28.295 | 258 | 161 | 6 | 6 | 242 | 14 | 268 | 2.29e-19 | 85.1 |
| MS.gene28849.t1 | AT2G29360 | 30.483 | 269 | 141 | 10 | 2 | 242 | 12 | 262 | 3.63e-19 | 84.7 |
| MS.gene28849.t1 | AT1G63380 | 28.571 | 259 | 159 | 8 | 6 | 242 | 19 | 273 | 4.85e-19 | 84.3 |
| MS.gene28849.t1 | AT1G63380 | 28.571 | 259 | 159 | 8 | 6 | 242 | 15 | 269 | 4.87e-19 | 84.3 |
| MS.gene28849.t1 | AT1G24360 | 26.667 | 255 | 164 | 8 | 4 | 242 | 72 | 319 | 5.39e-19 | 84.7 |
| MS.gene28849.t1 | AT1G63380 | 28.571 | 259 | 159 | 8 | 6 | 242 | 17 | 271 | 6.26e-19 | 84.0 |
| MS.gene28849.t1 | AT1G63380 | 28.571 | 259 | 159 | 8 | 6 | 242 | 22 | 276 | 7.95e-19 | 84.0 |
| MS.gene28849.t1 | AT2G30670 | 28.358 | 268 | 164 | 8 | 3 | 251 | 4 | 262 | 1.66e-18 | 82.4 |
| MS.gene28849.t1 | AT2G29350 | 29.963 | 267 | 144 | 10 | 2 | 242 | 11 | 260 | 3.05e-18 | 82.0 |
| MS.gene28849.t1 | AT5G06060 | 29.730 | 259 | 154 | 7 | 3 | 242 | 6 | 255 | 1.21e-17 | 80.1 |
| MS.gene28849.t1 | AT1G07440 | 27.547 | 265 | 151 | 8 | 3 | 242 | 9 | 257 | 5.14e-17 | 78.6 |
| MS.gene28849.t1 | AT2G29340 | 29.902 | 204 | 135 | 4 | 3 | 203 | 4 | 202 | 8.46e-17 | 76.6 |
| MS.gene28849.t1 | AT2G29340 | 29.902 | 204 | 135 | 4 | 3 | 203 | 4 | 202 | 8.46e-17 | 76.6 |
| MS.gene28849.t1 | AT3G46170 | 27.519 | 258 | 164 | 6 | 5 | 242 | 25 | 279 | 9.18e-17 | 78.2 |
| MS.gene28849.t1 | AT2G29330 | 27.626 | 257 | 162 | 6 | 3 | 242 | 4 | 253 | 1.52e-16 | 77.0 |
| MS.gene28849.t1 | AT2G29290 | 31.797 | 217 | 115 | 7 | 42 | 242 | 37 | 236 | 1.81e-16 | 76.6 |
| MS.gene28849.t1 | AT2G17845 | 28.889 | 270 | 148 | 10 | 6 | 243 | 47 | 304 | 2.70e-16 | 77.4 |
| MS.gene28849.t1 | AT2G29150 | 30.224 | 268 | 142 | 11 | 2 | 242 | 12 | 261 | 4.94e-16 | 75.9 |
| MS.gene28849.t1 | AT4G05530 | 31.008 | 258 | 145 | 8 | 3 | 240 | 6 | 250 | 5.37e-16 | 75.5 |
| MS.gene28849.t1 | AT2G29260 | 29.259 | 270 | 145 | 9 | 2 | 242 | 62 | 314 | 5.75e-16 | 76.3 |
| MS.gene28849.t1 | AT1G54870 | 26.718 | 262 | 162 | 7 | 5 | 244 | 83 | 336 | 2.05e-15 | 75.1 |
| MS.gene28849.t1 | AT2G29370 | 28.839 | 267 | 148 | 9 | 2 | 242 | 12 | 262 | 2.23e-15 | 73.9 |
| MS.gene28849.t1 | AT1G54870 | 26.718 | 262 | 162 | 7 | 5 | 244 | 81 | 334 | 2.50e-15 | 74.7 |
| MS.gene28849.t1 | AT2G29350 | 30.047 | 213 | 117 | 8 | 45 | 242 | 27 | 222 | 6.81e-15 | 72.0 |
| MS.gene28849.t1 | AT2G29300 | 28.044 | 271 | 162 | 11 | 3 | 251 | 4 | 263 | 1.56e-14 | 71.6 |
| MS.gene28849.t1 | AT1G49670 | 28.910 | 211 | 119 | 5 | 7 | 197 | 5 | 204 | 1.58e-14 | 73.2 |
| MS.gene28849.t1 | AT1G49670 | 28.910 | 211 | 119 | 5 | 7 | 197 | 5 | 204 | 1.70e-14 | 73.2 |
| MS.gene28849.t1 | AT2G29310 | 29.208 | 202 | 127 | 6 | 3 | 197 | 4 | 196 | 2.22e-14 | 70.1 |
| MS.gene28849.t1 | AT3G05260 | 26.154 | 260 | 162 | 6 | 5 | 242 | 36 | 287 | 3.46e-14 | 70.9 |
| MS.gene28849.t1 | AT2G29320 | 29.231 | 260 | 149 | 11 | 6 | 242 | 13 | 260 | 9.96e-14 | 69.3 |
| MS.gene28849.t1 | AT2G29300 | 27.969 | 261 | 155 | 11 | 3 | 241 | 4 | 253 | 1.25e-13 | 69.3 |
| MS.gene28849.t1 | AT2G29350 | 30.476 | 210 | 116 | 8 | 2 | 197 | 11 | 204 | 4.38e-13 | 67.0 |
| MS.gene28849.t1 | AT2G29310 | 26.829 | 246 | 152 | 7 | 25 | 251 | 24 | 260 | 6.33e-13 | 67.0 |
Find 55 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGTGGCTGGCTGTGTTGC+TGG | 0.229497 | 7.4:-5434402 | MS.gene28849:CDS |
| GTTAATTCAATTTCACCTTC+TGG | 0.256250 | 7.4:-5434288 | MS.gene28849:CDS |
| TTTATGTAGAGCACTGAATA+TGG | 0.259202 | 7.4:-5434250 | MS.gene28849:CDS |
| ACATCCAAACATGGTCTTAT+AGG | 0.260415 | 7.4:-5434357 | MS.gene28849:CDS |
| AGCTGATCCACGAACATTAA+TGG | 0.267172 | 7.4:+5434494 | None:intergenic |
| CACACTGGCCGTGCAAATTA+TGG | 0.271414 | 7.4:+5434419 | None:intergenic |
| GCAGATATCAATGATGAATT+TGG | 0.272072 | 7.4:-5434729 | MS.gene28849:CDS |
| GTAAAGACATTTGTGGAAAA+TGG | 0.275212 | 7.4:-5434768 | MS.gene28849:CDS |
| CACCAAGTTGCAACTTCAAT+TGG | 0.283127 | 7.4:-5434705 | MS.gene28849:CDS |
| TGCAGTGAGCTTGGAGCTTA+TGG | 0.289846 | 7.4:-5434318 | MS.gene28849:CDS |
| GGCTGTGTTGCTGGATGTGC+AGG | 0.325612 | 7.4:-5434393 | MS.gene28849:CDS |
| GATGAATCGGCTTATATTAG+TGG | 0.352269 | 7.4:-5434125 | MS.gene28849:CDS |
| CAAGCACGCTGCGCGTGTTA+TGG | 0.373393 | 7.4:-5434463 | MS.gene28849:CDS |
| AAGGCCTATAAGACCATGTT+TGG | 0.388328 | 7.4:+5434353 | None:intergenic |
| GTTTCTTGCTTCTGATGAAT+CGG | 0.388679 | 7.4:-5434138 | MS.gene28849:CDS |
| CGCTCGACGTGCAGTGAGCT+TGG | 0.402697 | 7.4:-5434327 | MS.gene28849:CDS |
| ATTGCTGAACATGATGTCTA+AGG | 0.405158 | 7.4:+5434593 | None:intergenic |
| ACTGGTGGAGCAAGCGGAAT+AGG | 0.405788 | 7.4:-5434801 | MS.gene28849:CDS |
| TTATATTAGTGGACAAAACT+TGG | 0.437085 | 7.4:-5434114 | MS.gene28849:CDS |
| GACATCATGTTCAGCAATGT+TGG | 0.440291 | 7.4:-5434588 | MS.gene28849:CDS |
| ACATCATGTTCAGCAATGTT+GGG | 0.442033 | 7.4:-5434587 | MS.gene28849:CDS |
| TTATGTAGAGCACTGAATAT+GGG | 0.444828 | 7.4:-5434249 | MS.gene28849:CDS |
| ACCAAGTTGCAACTTCAATT+GGG | 0.462862 | 7.4:-5434704 | MS.gene28849:CDS |
| CACTGCACGTCGAGCGAACA+AGG | 0.479956 | 7.4:+5434334 | None:intergenic |
| ATTTGCACGGCCAGTGTGGC+TGG | 0.483627 | 7.4:-5434414 | MS.gene28849:CDS |
| ATGTTCAGCAATGTTGGGAT+TGG | 0.487016 | 7.4:-5434582 | MS.gene28849:CDS |
| TTCAGCAATGTTGGGATTGG+AGG | 0.491721 | 7.4:-5434579 | MS.gene28849:CDS |
| ACCATGGCCATTAATGTTCG+TGG | 0.509551 | 7.4:-5434501 | MS.gene28849:CDS |
| TTTGAAAGGAATTACACTAA+AGG | 0.511106 | 7.4:-5434186 | MS.gene28849:intron |
| ACCCAATTGAAGTTGCAACT+TGG | 0.520892 | 7.4:+5434703 | None:intergenic |
| CAATTGAAGTTGCAACTTGG+TGG | 0.525100 | 7.4:+5434706 | None:intergenic |
| TTCAGTGCTCTACATAAAAG+AGG | 0.531671 | 7.4:+5434255 | None:intergenic |
| AGAGACAGTAAAGACATTTG+TGG | 0.542046 | 7.4:-5434775 | MS.gene28849:CDS |
| GGTAAGGTTGCTATAGTGAC+TGG | 0.551471 | 7.4:-5434819 | MS.gene28849:CDS |
| AGCAACACAGCCAGCCACAC+TGG | 0.557849 | 7.4:+5434404 | None:intergenic |
| TATGTAGAGCACTGAATATG+GGG | 0.559108 | 7.4:-5434248 | MS.gene28849:CDS |
| GGACAAAACTTGGTGGTCGA+TGG | 0.563116 | 7.4:-5434104 | MS.gene28849:CDS |
| GCACGCTGCGCGTGTTATGG+TGG | 0.567971 | 7.4:-5434460 | MS.gene28849:CDS |
| TCCACGAACATTAATGGCCA+TGG | 0.568390 | 7.4:+5434500 | None:intergenic |
| CAAAACTTGGTGGTCGATGG+AGG | 0.576243 | 7.4:-5434101 | MS.gene28849:CDS |
| TCGTGGATCCATAATTTGCA+CGG | 0.579852 | 7.4:-5434427 | MS.gene28849:CDS |
| CATAATTTGCACGGCCAGTG+TGG | 0.586134 | 7.4:-5434418 | MS.gene28849:CDS |
| AGAGGTGTGGCAACTCCAGA+AGG | 0.591710 | 7.4:+5434273 | None:intergenic |
| TATTAGTGGACAAAACTTGG+TGG | 0.609451 | 7.4:-5434111 | MS.gene28849:CDS |
| GAATAATCATGTCAAAACAA+AGG | 0.611671 | 7.4:-5434985 | None:intergenic |
| TTTCATCTCTCACGTCACAA+TGG | 0.615719 | 7.4:+5434661 | None:intergenic |
| ATAGTGACTGGTGGAGCAAG+CGG | 0.615867 | 7.4:-5434807 | MS.gene28849:CDS |
| CATCTCTCACGTCACAATGG+TGG | 0.625388 | 7.4:+5434664 | None:intergenic |
| TGCTCTACATAAAAGAGGTG+TGG | 0.627277 | 7.4:+5434260 | None:intergenic |
| AAGGTTGCTATAGTGACTGG+TGG | 0.636382 | 7.4:-5434816 | MS.gene28849:CDS |
| ATGTAGAGCACTGAATATGG+GGG | 0.636447 | 7.4:-5434247 | MS.gene28849:CDS |
| GCTTCACTGTCGTAAACCGA+TGG | 0.650644 | 7.4:-5434079 | MS.gene28849:CDS |
| GATTATACAACATCCAAACA+TGG | 0.670645 | 7.4:-5434366 | MS.gene28849:CDS |
| AAATGAATTTGACAACACCA+TGG | 0.693926 | 7.4:-5434517 | MS.gene28849:CDS |
| TGTAGAGCACTGAATATGGG+GGG | 0.706314 | 7.4:-5434246 | MS.gene28849:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATAGTATTCATTAAT+TGG | + | chr7.4:5434182-5434201 | None:intergenic | 10.0% |
| !!! | TGAATACTATATTTTTTCAT+AGG | - | chr7.4:5434187-5434206 | MS.gene28849:intron | 15.0% |
| !! | AAATATGTATATACACTACT+AGG | + | chr7.4:5434148-5434167 | None:intergenic | 20.0% |
| !!! | GTGTATATACATATTTCTAT+TGG | - | chr7.4:5434152-5434171 | MS.gene28849:CDS | 20.0% |
| !!! | TACTATATTTTTTCATAGGT+TGG | - | chr7.4:5434191-5434210 | MS.gene28849:intron | 20.0% |
| !!! | TTTCTACCTTTTTCTTTTTT+TGG | - | chr7.4:5434105-5434124 | MS.gene28849:CDS | 20.0% |
| ! | AATGATTCTGCTAATTTGAA+AGG | - | chr7.4:5434835-5434854 | MS.gene28849:intron | 25.0% |
| ! | GTTTGTCCAAAAAAAGAAAA+AGG | + | chr7.4:5434114-5434133 | None:intergenic | 25.0% |
| ! | TTTGAAAGGAATTACACTAA+AGG | - | chr7.4:5434849-5434868 | MS.gene28849:intron | 25.0% |
| !! | TTATATTAGTGGACAAAACT+TGG | - | chr7.4:5434921-5434940 | MS.gene28849:intron | 25.0% |
| !!! | ATATTTTTTCATAGGTTGGA+GGG | - | chr7.4:5434195-5434214 | MS.gene28849:intron | 25.0% |
| !!! | TATATTTTTTCATAGGTTGG+AGG | - | chr7.4:5434194-5434213 | MS.gene28849:intron | 25.0% |
| AAATGAATTTGACAACACCA+TGG | - | chr7.4:5434518-5434537 | MS.gene28849:CDS | 30.0% | |
| AGAAAAAGGTAGAAAGAAAG+AGG | + | chr7.4:5434100-5434119 | None:intergenic | 30.0% | |
| GAAAAAGGTAGAAAGAAAGA+GGG | + | chr7.4:5434099-5434118 | None:intergenic | 30.0% | |
| GATTATACAACATCCAAACA+TGG | - | chr7.4:5434669-5434688 | MS.gene28849:CDS | 30.0% | |
| GCAGATATCAATGATGAATT+TGG | - | chr7.4:5434306-5434325 | MS.gene28849:CDS | 30.0% | |
| GTAAAGACATTTGTGGAAAA+TGG | - | chr7.4:5434267-5434286 | MS.gene28849:CDS | 30.0% | |
| GTTAATTCAATTTCACCTTC+TGG | - | chr7.4:5434747-5434766 | MS.gene28849:CDS | 30.0% | |
| ! | GCTTTTGCATTGGAAAAATA+TGG | - | chr7.4:5434417-5434436 | MS.gene28849:CDS | 30.0% |
| ! | TTTTTCCAATGCAAAAGCTA+CGG | + | chr7.4:5434415-5434434 | None:intergenic | 30.0% |
| !! | TTATGTAGAGCACTGAATAT+GGG | - | chr7.4:5434786-5434805 | MS.gene28849:CDS | 30.0% |
| !! | TTTATGTAGAGCACTGAATA+TGG | - | chr7.4:5434785-5434804 | MS.gene28849:CDS | 30.0% |
| ACATCATGTTCAGCAATGTT+GGG | - | chr7.4:5434448-5434467 | MS.gene28849:CDS | 35.0% | |
| ACCAAGTTGCAACTTCAATT+GGG | - | chr7.4:5434331-5434350 | MS.gene28849:CDS | 35.0% | |
| AGAGACAGTAAAGACATTTG+TGG | - | chr7.4:5434260-5434279 | MS.gene28849:CDS | 35.0% | |
| ATTGCTGAACATGATGTCTA+AGG | + | chr7.4:5434445-5434464 | None:intergenic | 35.0% | |
| GAGAGATGAAAAACAAGTTG+AGG | - | chr7.4:5434386-5434405 | MS.gene28849:CDS | 35.0% | |
| GTTTCTTGCTTCTGATGAAT+CGG | - | chr7.4:5434897-5434916 | MS.gene28849:intron | 35.0% | |
| TGAGTGAAGTTGAAGCTATT+GGG | - | chr7.4:5434812-5434831 | MS.gene28849:CDS | 35.0% | |
| TTCAGTGCTCTACATAAAAG+AGG | + | chr7.4:5434783-5434802 | None:intergenic | 35.0% | |
| ! | ATGGTGGAAAGAAAAATTCG+TGG | - | chr7.4:5434591-5434610 | MS.gene28849:CDS | 35.0% |
| ! | GATGAATCGGCTTATATTAG+TGG | - | chr7.4:5434910-5434929 | MS.gene28849:intron | 35.0% |
| ! | TATTAGTGGACAAAACTTGG+TGG | - | chr7.4:5434924-5434943 | MS.gene28849:intron | 35.0% |
| !! | ACATCCAAACATGGTCTTAT+AGG | - | chr7.4:5434678-5434697 | MS.gene28849:CDS | 35.0% |
| !! | TATGTAGAGCACTGAATATG+GGG | - | chr7.4:5434787-5434806 | MS.gene28849:CDS | 35.0% |
| !!! | TAGCTTGTTTTTTACGCCAT+CGG | + | chr7.4:5434975-5434994 | None:intergenic | 35.0% |
| AAGGCCTATAAGACCATGTT+TGG | + | chr7.4:5434685-5434704 | None:intergenic | 40.0% | |
| ACCCAATTGAAGTTGCAACT+TGG | + | chr7.4:5434335-5434354 | None:intergenic | 40.0% | |
| AGCTGATCCACGAACATTAA+TGG | + | chr7.4:5434544-5434563 | None:intergenic | 40.0% | |
| ATGTTCAGCAATGTTGGGAT+TGG | - | chr7.4:5434453-5434472 | MS.gene28849:CDS | 40.0% | |
| CAATTGAAGTTGCAACTTGG+TGG | + | chr7.4:5434332-5434351 | None:intergenic | 40.0% | |
| CACCAAGTTGCAACTTCAAT+TGG | - | chr7.4:5434330-5434349 | MS.gene28849:CDS | 40.0% | |
| GACATCATGTTCAGCAATGT+TGG | - | chr7.4:5434447-5434466 | MS.gene28849:CDS | 40.0% | |
| GTGAGTGAAGTTGAAGCTAT+TGG | - | chr7.4:5434811-5434830 | MS.gene28849:CDS | 40.0% | |
| TGCTCTACATAAAAGAGGTG+TGG | + | chr7.4:5434778-5434797 | None:intergenic | 40.0% | |
| TTTCATCTCTCACGTCACAA+TGG | + | chr7.4:5434377-5434396 | None:intergenic | 40.0% | |
| ! | TCGTGGATCCATAATTTGCA+CGG | - | chr7.4:5434608-5434627 | MS.gene28849:CDS | 40.0% |
| !! | ATGTAGAGCACTGAATATGG+GGG | - | chr7.4:5434788-5434807 | MS.gene28849:CDS | 40.0% |
| !! | TTTTCATAGGTTGGAGGGTA+AGG | - | chr7.4:5434200-5434219 | MS.gene28849:intron | 40.0% |
| AAGGTTGCTATAGTGACTGG+TGG | - | chr7.4:5434219-5434238 | MS.gene28849:intron | 45.0% | |
| ACCATGGCCATTAATGTTCG+TGG | - | chr7.4:5434534-5434553 | MS.gene28849:CDS | 45.0% | |
| GGTAAGGTTGCTATAGTGAC+TGG | - | chr7.4:5434216-5434235 | MS.gene28849:intron | 45.0% | |
| TCCACGAACATTAATGGCCA+TGG | + | chr7.4:5434538-5434557 | None:intergenic | 45.0% | |
| TTCAGCAATGTTGGGATTGG+AGG | - | chr7.4:5434456-5434475 | MS.gene28849:CDS | 45.0% | |
| ! | GGAAACCGTAGCTTTTGCAT+TGG | - | chr7.4:5434407-5434426 | MS.gene28849:CDS | 45.0% |
| !! | TGTAGAGCACTGAATATGGG+GGG | - | chr7.4:5434789-5434808 | MS.gene28849:CDS | 45.0% |
| ATAGTGACTGGTGGAGCAAG+CGG | - | chr7.4:5434228-5434247 | MS.gene28849:intron | 50.0% | |
| CACACTGGCCGTGCAAATTA+TGG | + | chr7.4:5434619-5434638 | None:intergenic | 50.0% | |
| CATCTCTCACGTCACAATGG+TGG | + | chr7.4:5434374-5434393 | None:intergenic | 50.0% | |
| GCTTCACTGTCGTAAACCGA+TGG | - | chr7.4:5434956-5434975 | MS.gene28849:intron | 50.0% | |
| TGCAGTGAGCTTGGAGCTTA+TGG | - | chr7.4:5434717-5434736 | MS.gene28849:CDS | 50.0% | |
| ! | CAAAACTTGGTGGTCGATGG+AGG | - | chr7.4:5434934-5434953 | MS.gene28849:intron | 50.0% |
| ! | CATAATTTGCACGGCCAGTG+TGG | - | chr7.4:5434617-5434636 | MS.gene28849:CDS | 50.0% |
| ! | GGACAAAACTTGGTGGTCGA+TGG | - | chr7.4:5434931-5434950 | MS.gene28849:intron | 50.0% |
| ACTGGTGGAGCAAGCGGAAT+AGG | - | chr7.4:5434234-5434253 | MS.gene28849:intron | 55.0% | |
| ! | AGAGGTGTGGCAACTCCAGA+AGG | + | chr7.4:5434765-5434784 | None:intergenic | 55.0% |
| AGCAACACAGCCAGCCACAC+TGG | + | chr7.4:5434634-5434653 | None:intergenic | 60.0% | |
| ATTTGCACGGCCAGTGTGGC+TGG | - | chr7.4:5434621-5434640 | MS.gene28849:CDS | 60.0% | |
| CAAGCACGCTGCGCGTGTTA+TGG | - | chr7.4:5434572-5434591 | MS.gene28849:CDS | 60.0% | |
| CACTGCACGTCGAGCGAACA+AGG | + | chr7.4:5434704-5434723 | None:intergenic | 60.0% | |
| ! | AGTGTGGCTGGCTGTGTTGC+TGG | - | chr7.4:5434633-5434652 | MS.gene28849:CDS | 60.0% |
| ! | GGCTGTGTTGCTGGATGTGC+AGG | - | chr7.4:5434642-5434661 | MS.gene28849:CDS | 60.0% |
| CGCTCGACGTGCAGTGAGCT+TGG | - | chr7.4:5434708-5434727 | MS.gene28849:CDS | 65.0% | |
| GCACGCTGCGCGTGTTATGG+TGG | - | chr7.4:5434575-5434594 | MS.gene28849:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 5434058 | 5434999 | 5434058 | ID=MS.gene28849 |
| chr7.4 | mRNA | 5434058 | 5434999 | 5434058 | ID=MS.gene28849.t1;Parent=MS.gene28849 |
| chr7.4 | exon | 5434986 | 5434999 | 5434986 | ID=MS.gene28849.t1.exon1;Parent=MS.gene28849.t1 |
| chr7.4 | CDS | 5434986 | 5434999 | 5434986 | ID=cds.MS.gene28849.t1;Parent=MS.gene28849.t1 |
| chr7.4 | exon | 5434247 | 5434848 | 5434247 | ID=MS.gene28849.t1.exon2;Parent=MS.gene28849.t1 |
| chr7.4 | CDS | 5434247 | 5434848 | 5434247 | ID=cds.MS.gene28849.t1;Parent=MS.gene28849.t1 |
| chr7.4 | exon | 5434058 | 5434200 | 5434058 | ID=MS.gene28849.t1.exon3;Parent=MS.gene28849.t1 |
| chr7.4 | CDS | 5434058 | 5434200 | 5434058 | ID=cds.MS.gene28849.t1;Parent=MS.gene28849.t1 |
| Gene Sequence |
| Protein sequence |
