AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29019

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29019.t1 MTR_3g073640 98.526 475 6 1 1 475 1 474 0.0 962
MS.gene29019.t1 MTR_2g008440 62.914 453 161 3 25 475 25 472 0.0 574
MS.gene29019.t1 MTR_8g085600 25.901 444 235 14 41 475 75 433 9.13e-37 140
MS.gene29019.t1 MTR_4g114110 27.778 378 187 14 43 411 81 381 3.85e-32 127
MS.gene29019.t1 MTR_2g019230 27.174 368 193 13 21 382 32 330 4.25e-32 127
MS.gene29019.t1 MTR_8g080370 24.552 391 213 12 29 413 37 351 1.23e-21 97.1
MS.gene29019.t1 MTR_3g094850 23.121 346 186 7 38 382 4 270 2.27e-18 86.7
MS.gene29019.t1 MTR_3g085570 23.662 355 222 12 26 360 13 338 2.24e-17 84.0
MS.gene29019.t1 MTR_8g030940 23.057 386 223 14 29 410 7 322 3.98e-17 83.2
MS.gene29019.t1 MTR_4g022400 25.137 366 193 15 27 385 55 346 2.65e-16 81.3
MS.gene29019.t1 MTR_2g094093 24.571 350 212 13 14 348 2 314 3.21e-16 80.5
MS.gene29019.t1 MTR_0105s0130 22.857 315 199 9 34 326 69 361 8.36e-16 79.7
MS.gene29019.t1 MTR_0345s0010 22.857 315 199 9 34 326 69 361 8.51e-16 79.7
MS.gene29019.t1 MTR_1330s0010 22.857 315 199 9 34 326 82 374 1.01e-15 79.7
MS.gene29019.t1 MTR_4g022260 24.219 256 161 8 34 267 51 295 1.66e-15 79.0
MS.gene29019.t1 MTR_6g023805 25.311 241 156 7 44 267 57 290 1.85e-15 78.6
MS.gene29019.t1 MTR_0105s0020 22.540 315 200 9 34 326 69 361 1.91e-15 78.6
MS.gene29019.t1 MTR_0105s0170 22.857 315 199 9 34 326 82 374 2.53e-15 78.6
MS.gene29019.t1 MTR_7g029290 25.417 240 145 7 46 267 58 281 3.07e-15 77.8
MS.gene29019.t1 MTR_0496s0010 23.438 256 163 8 34 267 51 295 3.17e-15 77.8
MS.gene29019.t1 MTR_0105s0040 22.857 315 199 9 34 326 167 459 3.36e-15 78.2
MS.gene29019.t1 MTR_4g108280 23.797 374 204 14 23 385 9 312 1.77e-14 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29019.t1 AT1G22040 63.941 477 164 5 1 475 1 471 0.0 592
MS.gene29019.t1 AT1G55270 25.821 457 241 15 28 475 63 430 7.73e-36 138
MS.gene29019.t1 AT1G30090 24.800 375 206 14 43 411 53 357 2.43e-28 116
MS.gene29019.t1 AT1G55270 25.510 392 194 15 93 475 2 304 7.63e-23 99.0
MS.gene29019.t1 AT2G24540 24.051 395 229 12 16 408 2 327 2.73e-21 95.9
MS.gene29019.t1 AT1G26930 25.984 254 173 6 43 287 71 318 6.21e-19 89.4
MS.gene29019.t1 AT2G21950 24.348 345 186 10 42 382 19 292 2.18e-17 84.0
MS.gene29019.t1 AT4G11770 28.511 235 151 7 38 266 6 229 3.58e-16 80.9
MS.gene29019.t1 AT1G19460 23.262 374 205 14 44 408 56 356 4.42e-16 80.5
MS.gene29019.t1 AT3G61350 25.074 339 168 15 30 359 9 270 9.18e-16 79.0
MS.gene29019.t1 AT3G61350 25.074 339 168 15 30 359 9 270 9.18e-16 79.0
MS.gene29019.t1 AT4G39550 25.444 338 200 10 45 382 25 310 1.26e-15 79.0
MS.gene29019.t1 AT1G19470 23.947 380 204 15 44 414 56 359 1.98e-15 78.6
MS.gene29019.t1 AT3G27150 25.185 270 161 9 45 289 72 325 7.48e-15 77.0
MS.gene29019.t1 AT3G27150 25.185 270 161 9 45 289 72 325 7.48e-15 77.0
MS.gene29019.t1 AT5G49000 28.216 241 135 7 28 266 5 209 1.36e-14 75.5
MS.gene29019.t1 AT1G26930 25.203 246 133 6 43 287 71 266 1.56e-14 75.5
MS.gene29019.t1 AT1G74510 24.047 341 166 14 64 385 116 382 4.29e-13 71.6
MS.gene29019.t1 AT1G74510 24.047 341 166 14 64 385 116 382 4.29e-13 71.6
MS.gene29019.t1 AT1G74510 24.047 341 166 14 64 385 116 382 4.29e-13 71.6
MS.gene29019.t1 AT3G06570 27.149 221 128 5 47 265 29 218 8.77e-13 70.1
MS.gene29019.t1 AT5G60570 23.810 231 163 4 43 267 49 272 1.38e-12 69.7
MS.gene29019.t1 AT5G60570 23.810 231 163 4 43 267 49 272 1.38e-12 69.7
MS.gene29019.t1 AT5G60570 23.810 231 163 4 43 267 49 272 1.38e-12 69.7
MS.gene29019.t1 AT5G38680 27.706 231 134 6 45 273 17 216 9.39e-12 67.0
MS.gene29019.t1 AT5G49000 28.436 211 120 7 28 236 5 186 1.64e-11 65.9
MS.gene29019.t1 AT4G39600 27.149 221 127 6 45 265 15 201 2.02e-11 65.9
MS.gene29019.t1 AT4G39600 27.149 221 127 6 45 265 26 212 2.06e-11 65.9
MS.gene29019.t1 AT2G44130 25.773 291 179 11 43 325 19 280 3.96e-11 65.1

Find 122 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATCCATGAGTTTGTTTC+AGG 0.204153 3.2:+66400281 None:intergenic
GGCCGAGCTGCGCAGTAATT+TGG 0.229110 3.2:-66399979 MS.gene29019:CDS
GGTTCTTCCAAATGCTTTCT+TGG 0.236360 3.2:-66400420 MS.gene29019:CDS
TTCCACAAAGTTTATATTCA+TGG 0.271003 3.2:-66400340 MS.gene29019:CDS
GAAGTTATTAGAGGCTTTCT+TGG 0.279878 3.2:-66400773 MS.gene29019:CDS
TGATGAAGAAGAATCTAAAA+AGG 0.280259 3.2:-66400849 MS.gene29019:CDS
AGAATTGCTGAAGTTATTAG+AGG 0.298500 3.2:-66400782 MS.gene29019:CDS
GAGTCTCCTTATCTACTTGC+TGG 0.303026 3.2:-66400062 MS.gene29019:CDS
GATAGTTGCTAGTAGGTGTT+TGG 0.310903 3.2:-66399862 MS.gene29019:CDS
AGGGTTCATCTTCTAGGCTT+TGG 0.313263 3.2:-66400829 MS.gene29019:CDS
AATAACAAGCTTTATGTTGT+TGG 0.331129 3.2:-66400554 MS.gene29019:CDS
GCTGTATTTGTTGGTTAAGT+TGG 0.334835 3.2:-66400951 MS.gene29019:CDS
ATTGGAAAAGTCCCTATATA+TGG 0.351212 3.2:-66400098 MS.gene29019:CDS
GATACCTCATCAGGGAGATT+TGG 0.365248 3.2:+66401106 None:intergenic
GAATCATCAGAACCATATAT+AGG 0.378338 3.2:+66400086 None:intergenic
CCGAAACAGACGCAGTTGTT+TGG 0.378938 3.2:-66399887 MS.gene29019:CDS
TGCTAGTAGGTGTTTGGGAC+AGG 0.379732 3.2:-66399856 MS.gene29019:CDS
CTGTATTTGTTGGTTAAGTT+GGG 0.385502 3.2:-66400950 MS.gene29019:CDS
CCAAACAACTGCGTCTGTTT+CGG 0.386041 3.2:+66399887 None:intergenic
AATCATCAGAACCATATATA+GGG 0.398150 3.2:+66400087 None:intergenic
ATCTGAATCGATACCTCATC+AGG 0.403634 3.2:+66401097 None:intergenic
CACAGGTTTGACAGCATACA+TGG 0.407493 3.2:-66400375 MS.gene29019:CDS
TATCTACTTGCTGGTTTCCA+TGG 0.416465 3.2:-66400053 MS.gene29019:CDS
TCTTCTTCATCAACAACATT+AGG 0.419662 3.2:+66400860 None:intergenic
TAGATCCGAGATCGAGGATA+TGG 0.422429 3.2:-66400901 MS.gene29019:CDS
GCTCTCGAAAACGGCATACT+AGG 0.424644 3.2:+66400446 None:intergenic
CCTTCCACTGTCCATAGTAT+TGG 0.428663 3.2:+66400163 None:intergenic
CATCTTCTAGGCTTTGGATG+TGG 0.429788 3.2:-66400823 MS.gene29019:CDS
GGCCATGAATATAAACTTTG+TGG 0.433006 3.2:+66400338 None:intergenic
GGGAGATTTGGAAACAGTCT+TGG 0.433358 3.2:+66401118 None:intergenic
GATGCTTGGAAAGTTGTCAT+TGG 0.434345 3.2:-66400116 MS.gene29019:CDS
ATGTCGGCCAAGAAAGCATT+TGG 0.438307 3.2:+66400413 None:intergenic
GGAAGAGTGGCTGTATTTGT+TGG 0.438529 3.2:-66400960 MS.gene29019:CDS
TCAACAACATTAGGCATAGT+AGG 0.441980 3.2:+66400869 None:intergenic
GACTTCTATGTCTATTGGTC+GGG 0.449376 3.2:-66400600 MS.gene29019:CDS
GATAGAAATGCCACTTGGAA+TGG 0.451326 3.2:-66400264 MS.gene29019:CDS
TTAGTACCTGCTTGCCGTGC+GGG 0.453647 3.2:+66400230 None:intergenic
TTTGGAAGATAGTTGCTAGT+AGG 0.459506 3.2:-66399869 MS.gene29019:CDS
TCCAATAGCACAGCCGCAAA+AGG 0.467282 3.2:+66400724 None:intergenic
ATAGTTGCTAGTAGGTGTTT+GGG 0.476162 3.2:-66399861 MS.gene29019:CDS
AAGTTCCTTACAAGAATTTG+CGG 0.476864 3.2:-66399919 MS.gene29019:CDS
TCATAACATTGCTGTTCTGC+AGG 0.486624 3.2:-66400000 MS.gene29019:CDS
CTTCAACTATGAAATGTGTC+TGG 0.488133 3.2:-66400658 MS.gene29019:CDS
GGCTGTGCTATTGGAGCTGT+TGG 0.490714 3.2:-66400716 MS.gene29019:CDS
TGTGGCGAAGAATACTAATT+CGG 0.492174 3.2:-66401221 MS.gene29019:CDS
CTGCGTCTGTTTCGGCCGGC+GGG 0.492966 3.2:+66399895 None:intergenic
TGAGGATGGTGTCGAGGAAG+TGG 0.493675 3.2:-66401039 MS.gene29019:CDS
GAAGTCGACTGTCACGAGTT+CGG 0.494689 3.2:-66401017 MS.gene29019:CDS
TTGATCCCTCGACAGGTACA+TGG 0.494889 3.2:-66400478 MS.gene29019:CDS
ACATTGAAGTAACAAATTCT+CGG 0.495616 3.2:+66401061 None:intergenic
TTAGAGGCTTTCTTGGAAAG+AGG 0.497593 3.2:-66400766 MS.gene29019:CDS
ATGTTAAAGCCGATTGCCAC+AGG 0.497640 3.2:-66400392 MS.gene29019:CDS
CGGGTCCGCAAATTCTTGTA+AGG 0.501840 3.2:+66399914 None:intergenic
GGTCGGGCATACTGTAAGAC+AGG 0.502459 3.2:-66400584 MS.gene29019:CDS
AACAAGCTTTATGTTGTTGG+AGG 0.503886 3.2:-66400551 MS.gene29019:CDS
CACTTGGAATGGGCGAAGGT+TGG 0.505733 3.2:-66400253 MS.gene29019:CDS
GGTTGGCCCGCACGGCAAGC+AGG 0.507113 3.2:-66400236 MS.gene29019:CDS
GCTGTCAAACCTGTGGCAAT+CGG 0.509192 3.2:+66400383 None:intergenic
CCAATAGCACAGCCGCAAAA+GGG 0.509237 3.2:+66400725 None:intergenic
TAGAGGCTTTCTTGGAAAGA+GGG 0.509564 3.2:-66400765 MS.gene29019:CDS
ATGATCAAGGAGAAGATGCT+TGG 0.511442 3.2:-66400130 MS.gene29019:CDS
ACTGCGTCTGTTTCGGCCGG+CGG 0.511651 3.2:+66399894 None:intergenic
AGAACCTGAGCTCTCGAAAA+CGG 0.513808 3.2:+66400437 None:intergenic
CAAGCTTTATGTTGTTGGAG+GGG 0.516776 3.2:-66400549 MS.gene29019:CDS
TGGGCGAAGGTTGGCCCGCA+CGG 0.518716 3.2:-66400244 MS.gene29019:CDS
CTCTGCCATATCCTCGATCT+CGG 0.519593 3.2:+66400896 None:intergenic
ATAGAAATGCCACTTGGAAT+GGG 0.519646 3.2:-66400263 MS.gene29019:CDS
TTGATCCGATGCAGAATACA+TGG 0.520704 3.2:-66400631 MS.gene29019:CDS
ATCCAAATTACTGCGCAGCT+CGG 0.524747 3.2:+66399977 None:intergenic
CTTTGAATGATACATGTAAG+AGG 0.528568 3.2:-66401174 MS.gene29019:CDS
GGGGTTAGTCAAGGTCCAGC+TGG 0.528816 3.2:-66400530 MS.gene29019:CDS
ACAAGCTTTATGTTGTTGGA+GGG 0.533435 3.2:-66400550 MS.gene29019:CDS
AGTGTTGATGAGGGTGATGA+TGG 0.533910 3.2:+66399948 None:intergenic
GTTGTTGGAGGGGTTAGTCA+AGG 0.535118 3.2:-66400539 MS.gene29019:CDS
TATAAAGTAAGAAAAGAACT+AGG 0.535380 3.2:-66400989 MS.gene29019:CDS
AAGGTGACTTCTATGTCTAT+TGG 0.543860 3.2:-66400605 MS.gene29019:CDS
AGGATCAAGGTATATGATCA+AGG 0.543954 3.2:-66400143 MS.gene29019:CDS
TGGAAACCAGCAAGTAGATA+AGG 0.544164 3.2:+66400056 None:intergenic
TGACTTCTATGTCTATTGGT+CGG 0.545501 3.2:-66400601 MS.gene29019:CDS
TATCCTCGATCTCGGATCTA+CGG 0.546450 3.2:+66400904 None:intergenic
ATGATCCTGAAACAAACTCA+TGG 0.549672 3.2:-66400286 MS.gene29019:CDS
TGAAGAGGTATCAATCCAGC+TGG 0.549753 3.2:+66400515 None:intergenic
TTGCTCCATGTATTCTGCAT+CGG 0.552823 3.2:+66400626 None:intergenic
TATGGACAGTGGAAGGATCA+AGG 0.557539 3.2:-66400156 MS.gene29019:CDS
TAGGCTTTGGATGTGGAACA+TGG 0.558491 3.2:-66400816 MS.gene29019:CDS
ACAAGAATTTGCGGACCCGC+CGG 0.560358 3.2:-66399910 MS.gene29019:CDS
TTGTTACTTCAATGTGAGGA+TGG 0.575358 3.2:-66401053 MS.gene29019:CDS
ACAACTGCGTCTGTTTCGGC+CGG 0.576574 3.2:+66399891 None:intergenic
CCAATACTATGGACAGTGGA+AGG 0.580690 3.2:-66400163 MS.gene29019:CDS
AAATTGAGTGTCGTAGTCGA+TGG 0.583833 3.2:-66400209 MS.gene29019:CDS
TGTGCTATTGGAGCTGTTGG+TGG 0.583999 3.2:-66400713 MS.gene29019:CDS
GAATTTGTTACTTCAATGTG+AGG 0.591869 3.2:-66401057 MS.gene29019:CDS
ATAAAGTAAGAAAAGAACTA+GGG 0.591911 3.2:-66400988 MS.gene29019:CDS
TCTTGGATTCTCTTCATCAA+CGG 0.595585 3.2:+66401135 None:intergenic
GGCAATCGGCTTTAACATGT+CGG 0.596973 3.2:+66400397 None:intergenic
ATGCCACTTGGAATGGGCGA+AGG 0.602736 3.2:-66400257 MS.gene29019:CDS
TCATGGATAGAAATGCCACT+TGG 0.603202 3.2:-66400269 MS.gene29019:CDS
AACTAGGGACGACGGAAGAG+TGG 0.605467 3.2:-66400973 MS.gene29019:CDS
GTCTGACCATGTACCTGTCG+AGG 0.606013 3.2:+66400472 None:intergenic
CCACTGTCCATAGTATTGGA+AGG 0.612205 3.2:+66400167 None:intergenic
AAAACTTCAGCAGATTGAAG+AGG 0.613948 3.2:+66400500 None:intergenic
GTTTCCAAATCTCCCTGATG+AGG 0.618301 3.2:-66401110 MS.gene29019:CDS
TTTAGTACCTGCTTGCCGTG+CGG 0.623460 3.2:+66400229 None:intergenic
ATGCCGTAGATCCGAGATCG+AGG 0.624971 3.2:-66400907 MS.gene29019:CDS
TCGGATCAAAGTGAGTGGGA+TGG 0.635544 3.2:-66401202 MS.gene29019:CDS
GTTTGACAGCATACATGGGA+AGG 0.643241 3.2:-66400370 MS.gene29019:CDS
CTAATTCGGATCAAAGTGAG+TGG 0.647666 3.2:-66401207 MS.gene29019:CDS
TAATTCGGATCAAAGTGAGT+GGG 0.647966 3.2:-66401206 MS.gene29019:CDS
GATGCAGAATACATGGAGCA+AGG 0.650802 3.2:-66400624 MS.gene29019:CDS
CCTTCCAATACTATGGACAG+TGG 0.652140 3.2:-66400167 MS.gene29019:CDS
ACTTTGTGACAGTGTTGATG+AGG 0.658181 3.2:+66399938 None:intergenic
AAGAAAAGAACTAGGGACGA+CGG 0.661261 3.2:-66400981 MS.gene29019:CDS
CTTTGTGACAGTGTTGATGA+GGG 0.663670 3.2:+66399939 None:intergenic
TCTGACCATGTACCTGTCGA+GGG 0.664162 3.2:+66400473 None:intergenic
TCAATGTGAGGATGGTGTCG+AGG 0.665635 3.2:-66401045 MS.gene29019:CDS
TCTGAATCGATACCTCATCA+GGG 0.669169 3.2:+66401098 None:intergenic
CAACCTTCGCCCATTCCAAG+TGG 0.676112 3.2:+66400254 None:intergenic
ACAGGTTTGACAGCATACAT+GGG 0.691995 3.2:-66400374 MS.gene29019:CDS
GTCGGGCATACTGTAAGACA+GGG 0.692352 3.2:-66400583 MS.gene29019:CDS
TGGATGTGGAACATGGTGGA+AGG 0.693870 3.2:-66400809 MS.gene29019:CDS
CATGTATGCTGTCAAACCTG+TGG 0.714854 3.2:+66400376 None:intergenic
GCTTTGGATGTGGAACATGG+TGG 0.780393 3.2:-66400813 MS.gene29019:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAGTAAGAAAAGAACTA+GGG - chr3.2:66400086-66400105 MS.gene29019:CDS 20.0%
!! TATAAAGTAAGAAAAGAACT+AGG - chr3.2:66400085-66400104 MS.gene29019:CDS 20.0%
! AATCATCAGAACCATATATA+GGG + chr3.2:66400990-66401009 None:intergenic 25.0%
! ACATTGAAGTAACAAATTCT+CGG + chr3.2:66400016-66400035 None:intergenic 25.0%
! GATGAAGAAGAATCTAAAAA+GGG - chr3.2:66400226-66400245 MS.gene29019:CDS 25.0%
! TGATGAAGAAGAATCTAAAA+AGG - chr3.2:66400225-66400244 MS.gene29019:CDS 25.0%
! TTCCACAAAGTTTATATTCA+TGG - chr3.2:66400734-66400753 MS.gene29019:CDS 25.0%
!! AATAACAAGCTTTATGTTGT+TGG - chr3.2:66400520-66400539 MS.gene29019:CDS 25.0%
AAGTTCCTTACAAGAATTTG+CGG - chr3.2:66401155-66401174 MS.gene29019:CDS 30.0%
AGAATTGCTGAAGTTATTAG+AGG - chr3.2:66400292-66400311 MS.gene29019:CDS 30.0%
ATTGGAAAAGTCCCTATATA+TGG - chr3.2:66400976-66400995 MS.gene29019:CDS 30.0%
CTTTGAATGATACATGTAAG+AGG - chr3.2:66399900-66399919 MS.gene29019:CDS 30.0%
GAATCATCAGAACCATATAT+AGG + chr3.2:66400991-66401010 None:intergenic 30.0%
TCTTCTTCATCAACAACATT+AGG + chr3.2:66400217-66400236 None:intergenic 30.0%
! CTGTATTTGTTGGTTAAGTT+GGG - chr3.2:66400124-66400143 MS.gene29019:CDS 30.0%
! TTTTGATCCTTCCAATACTA+TGG - chr3.2:66400900-66400919 MS.gene29019:CDS 30.0%
!! GAATTTGTTACTTCAATGTG+AGG - chr3.2:66400017-66400036 MS.gene29019:CDS 30.0%
AAAACTTCAGCAGATTGAAG+AGG + chr3.2:66400577-66400596 None:intergenic 35.0%
AAGGTGACTTCTATGTCTAT+TGG - chr3.2:66400469-66400488 MS.gene29019:CDS 35.0%
AGGATCAAGGTATATGATCA+AGG - chr3.2:66400931-66400950 MS.gene29019:CDS 35.0%
ATAGTTGCTAGTAGGTGTTT+GGG - chr3.2:66401213-66401232 MS.gene29019:CDS 35.0%
ATGATCCTGAAACAAACTCA+TGG - chr3.2:66400788-66400807 MS.gene29019:CDS 35.0%
CCTCCAACATCAACAAAAAA+GGG + chr3.2:66400760-66400779 None:intergenic 35.0%
CTAAAAAGGGTTCATCTTCT+AGG - chr3.2:66400239-66400258 MS.gene29019:CDS 35.0%
CTTCAACTATGAAATGTGTC+TGG - chr3.2:66400416-66400435 MS.gene29019:CDS 35.0%
GAAGTTATTAGAGGCTTTCT+TGG - chr3.2:66400301-66400320 MS.gene29019:CDS 35.0%
GGCCATGAATATAAACTTTG+TGG + chr3.2:66400739-66400758 None:intergenic 35.0%
TAATTCGGATCAAAGTGAGT+GGG - chr3.2:66399868-66399887 MS.gene29019:CDS 35.0%
TCAACAACATTAGGCATAGT+AGG + chr3.2:66400208-66400227 None:intergenic 35.0%
TCCTCCAACATCAACAAAAA+AGG + chr3.2:66400761-66400780 None:intergenic 35.0%
TCTTGGATTCTCTTCATCAA+CGG + chr3.2:66399942-66399961 None:intergenic 35.0%
TGACTTCTATGTCTATTGGT+CGG - chr3.2:66400473-66400492 MS.gene29019:CDS 35.0%
TGTGGCGAAGAATACTAATT+CGG - chr3.2:66399853-66399872 MS.gene29019:CDS 35.0%
TTTGGAAGATAGTTGCTAGT+AGG - chr3.2:66401205-66401224 MS.gene29019:CDS 35.0%
! AACAAGCTTTATGTTGTTGG+AGG - chr3.2:66400523-66400542 MS.gene29019:CDS 35.0%
! ACAAGCTTTATGTTGTTGGA+GGG - chr3.2:66400524-66400543 MS.gene29019:CDS 35.0%
! ATAGAAATGCCACTTGGAAT+GGG - chr3.2:66400811-66400830 MS.gene29019:CDS 35.0%
! GCTGTATTTGTTGGTTAAGT+TGG - chr3.2:66400123-66400142 MS.gene29019:CDS 35.0%
! TCTATCCATGAGTTTGTTTC+AGG + chr3.2:66400796-66400815 None:intergenic 35.0%
! TGATGACATGAAGTTTTCCA+TGG + chr3.2:66401041-66401060 None:intergenic 35.0%
! TGGGAGAAAATAAGCTTTTG+TGG - chr3.2:66400143-66400162 MS.gene29019:CDS 35.0%
! TTGTTACTTCAATGTGAGGA+TGG - chr3.2:66400021-66400040 MS.gene29019:CDS 35.0%
! TTTGATGAAATGCCCTTTTG+CGG - chr3.2:66400337-66400356 MS.gene29019:CDS 35.0%
AAGAAAAGAACTAGGGACGA+CGG - chr3.2:66400093-66400112 MS.gene29019:CDS 40.0%
ACAGGTTTGACAGCATACAT+GGG - chr3.2:66400700-66400719 MS.gene29019:CDS 40.0%
ATCTGAATCGATACCTCATC+AGG + chr3.2:66399980-66399999 None:intergenic 40.0%
ATGATCAAGGAGAAGATGCT+TGG - chr3.2:66400944-66400963 MS.gene29019:CDS 40.0%
CTAATTCGGATCAAAGTGAG+TGG - chr3.2:66399867-66399886 MS.gene29019:CDS 40.0%
GACTTCTATGTCTATTGGTC+GGG - chr3.2:66400474-66400493 MS.gene29019:CDS 40.0%
GATAGTTGCTAGTAGGTGTT+TGG - chr3.2:66401212-66401231 MS.gene29019:CDS 40.0%
GATGCTTGGAAAGTTGTCAT+TGG - chr3.2:66400958-66400977 MS.gene29019:CDS 40.0%
GGTTCTTCCAAATGCTTTCT+TGG - chr3.2:66400654-66400673 MS.gene29019:CDS 40.0%
TAGAGGCTTTCTTGGAAAGA+GGG - chr3.2:66400309-66400328 MS.gene29019:CDS 40.0%
TCATGGATAGAAATGCCACT+TGG - chr3.2:66400805-66400824 MS.gene29019:CDS 40.0%
TCTGAATCGATACCTCATCA+GGG + chr3.2:66399979-66399998 None:intergenic 40.0%
TGGAAACCAGCAAGTAGATA+AGG + chr3.2:66401021-66401040 None:intergenic 40.0%
TTAGAGGCTTTCTTGGAAAG+AGG - chr3.2:66400308-66400327 MS.gene29019:CDS 40.0%
TTGATCCGATGCAGAATACA+TGG - chr3.2:66400443-66400462 MS.gene29019:CDS 40.0%
TTGCTCCATGTATTCTGCAT+CGG + chr3.2:66400451-66400470 None:intergenic 40.0%
! AAATTGAGTGTCGTAGTCGA+TGG - chr3.2:66400865-66400884 MS.gene29019:CDS 40.0%
! CAAGCTTTATGTTGTTGGAG+GGG - chr3.2:66400525-66400544 MS.gene29019:CDS 40.0%
! GATAGAAATGCCACTTGGAA+TGG - chr3.2:66400810-66400829 MS.gene29019:CDS 40.0%
! GGTGGATGTCTCTATGTTTT+AGG - chr3.2:66400379-66400398 MS.gene29019:CDS 40.0%
! TCATAACATTGCTGTTCTGC+AGG - chr3.2:66401074-66401093 MS.gene29019:CDS 40.0%
!! ACTTTGTGACAGTGTTGATG+AGG + chr3.2:66401139-66401158 None:intergenic 40.0%
!! CTTTGTGACAGTGTTGATGA+GGG + chr3.2:66401138-66401157 None:intergenic 40.0%
!! GGATGTCTCTATGTTTTAGG+TGG - chr3.2:66400382-66400401 MS.gene29019:CDS 40.0%
!! TATCTACTTGCTGGTTTCCA+TGG - chr3.2:66401021-66401040 MS.gene29019:CDS 40.0%
!!! CCCTTTTTTGTTGATGTTGG+AGG - chr3.2:66400757-66400776 MS.gene29019:CDS 40.0%
!!! TGGCCCTTTTTTGTTGATGT+TGG - chr3.2:66400754-66400773 MS.gene29019:CDS 40.0%
AGAACCTGAGCTCTCGAAAA+CGG + chr3.2:66400640-66400659 None:intergenic 45.0%
AGGGTTCATCTTCTAGGCTT+TGG - chr3.2:66400245-66400264 MS.gene29019:CDS 45.0%
ATCCAAATTACTGCGCAGCT+CGG + chr3.2:66401100-66401119 None:intergenic 45.0%
ATGTTAAAGCCGATTGCCAC+AGG - chr3.2:66400682-66400701 MS.gene29019:CDS 45.0%
CACAGGTTTGACAGCATACA+TGG - chr3.2:66400699-66400718 MS.gene29019:CDS 45.0%
CATGTATGCTGTCAAACCTG+TGG + chr3.2:66400701-66400720 None:intergenic 45.0%
CCAAACAACTGCGTCTGTTT+CGG + chr3.2:66401190-66401209 None:intergenic 45.0%
CCAATACTATGGACAGTGGA+AGG - chr3.2:66400911-66400930 MS.gene29019:CDS 45.0%
CCTTCCAATACTATGGACAG+TGG - chr3.2:66400907-66400926 MS.gene29019:CDS 45.0%
CCTTCCACTGTCCATAGTAT+TGG + chr3.2:66400914-66400933 None:intergenic 45.0%
GAGTCTCCTTATCTACTTGC+TGG - chr3.2:66401012-66401031 MS.gene29019:CDS 45.0%
GATACCTCATCAGGGAGATT+TGG + chr3.2:66399971-66399990 None:intergenic 45.0%
GATGCAGAATACATGGAGCA+AGG - chr3.2:66400450-66400469 MS.gene29019:CDS 45.0%
GGAAGAGTGGCTGTATTTGT+TGG - chr3.2:66400114-66400133 MS.gene29019:CDS 45.0%
GGGAGATTTGGAAACAGTCT+TGG + chr3.2:66399959-66399978 None:intergenic 45.0%
GTTTCCAAATCTCCCTGATG+AGG - chr3.2:66399964-66399983 MS.gene29019:CDS 45.0%
GTTTGACAGCATACATGGGA+AGG - chr3.2:66400704-66400723 MS.gene29019:CDS 45.0%
TAGATCCGAGATCGAGGATA+TGG - chr3.2:66400173-66400192 MS.gene29019:CDS 45.0%
TATCCTCGATCTCGGATCTA+CGG + chr3.2:66400173-66400192 None:intergenic 45.0%
TATGGACAGTGGAAGGATCA+AGG - chr3.2:66400918-66400937 MS.gene29019:CDS 45.0%
! CCACTGTCCATAGTATTGGA+AGG + chr3.2:66400910-66400929 None:intergenic 45.0%
! GAAGTTTTTGATCCCTCGAC+AGG - chr3.2:66400589-66400608 MS.gene29019:CDS 45.0%
! GGCAATCGGCTTTAACATGT+CGG + chr3.2:66400680-66400699 None:intergenic 45.0%
!! AGTGTTGATGAGGGTGATGA+TGG + chr3.2:66401129-66401148 None:intergenic 45.0%
!! ATGTCGGCCAAGAAAGCATT+TGG + chr3.2:66400664-66400683 None:intergenic 45.0%
!! CATCTTCTAGGCTTTGGATG+TGG - chr3.2:66400251-66400270 MS.gene29019:CDS 45.0%
!! TAGGCTTTGGATGTGGAACA+TGG - chr3.2:66400258-66400277 MS.gene29019:CDS 45.0%
!! TGAAGAGGTATCAATCCAGC+TGG + chr3.2:66400562-66400581 None:intergenic 45.0%
CCAATAGCACAGCCGCAAAA+GGG + chr3.2:66400352-66400371 None:intergenic 50.0%
CCGAAACAGACGCAGTTGTT+TGG - chr3.2:66401187-66401206 MS.gene29019:CDS 50.0%
CGGGTCCGCAAATTCTTGTA+AGG + chr3.2:66401163-66401182 None:intergenic 50.0%
CTCTGCCATATCCTCGATCT+CGG + chr3.2:66400181-66400200 None:intergenic 50.0%
GAAGTCGACTGTCACGAGTT+CGG - chr3.2:66400057-66400076 MS.gene29019:CDS 50.0%
GCTCTCGAAAACGGCATACT+AGG + chr3.2:66400631-66400650 None:intergenic 50.0%
GCTGTCAAACCTGTGGCAAT+CGG + chr3.2:66400694-66400713 None:intergenic 50.0%
GTCGGGCATACTGTAAGACA+GGG - chr3.2:66400491-66400510 MS.gene29019:CDS 50.0%
TCCAATAGCACAGCCGCAAA+AGG + chr3.2:66400353-66400372 None:intergenic 50.0%
TCGGATCAAAGTGAGTGGGA+TGG - chr3.2:66399872-66399891 MS.gene29019:CDS 50.0%
TCTGACCATGTACCTGTCGA+GGG + chr3.2:66400604-66400623 None:intergenic 50.0%
TTGATCCCTCGACAGGTACA+TGG - chr3.2:66400596-66400615 MS.gene29019:CDS 50.0%
TTTAGTACCTGCTTGCCGTG+CGG + chr3.2:66400848-66400867 None:intergenic 50.0%
! GCTTTGGATGTGGAACATGG+TGG - chr3.2:66400261-66400280 MS.gene29019:CDS 50.0%
! TCAATGTGAGGATGGTGTCG+AGG - chr3.2:66400029-66400048 MS.gene29019:CDS 50.0%
! TGCTAGTAGGTGTTTGGGAC+AGG - chr3.2:66401218-66401237 MS.gene29019:CDS 50.0%
! TGGATGTGGAACATGGTGGA+AGG - chr3.2:66400265-66400284 MS.gene29019:CDS 50.0%
! TGTGCTATTGGAGCTGTTGG+TGG - chr3.2:66400361-66400380 MS.gene29019:CDS 50.0%
!! GTTGTTGGAGGGGTTAGTCA+AGG - chr3.2:66400535-66400554 MS.gene29019:CDS 50.0%
!!! TATGCCGTTTTCGAGAGCTC+AGG - chr3.2:66400633-66400652 MS.gene29019:CDS 50.0%
AACTAGGGACGACGGAAGAG+TGG - chr3.2:66400101-66400120 MS.gene29019:CDS 55.0%
ACAACTGCGTCTGTTTCGGC+CGG + chr3.2:66401186-66401205 None:intergenic 55.0%
ACAAGAATTTGCGGACCCGC+CGG - chr3.2:66401164-66401183 MS.gene29019:CDS 55.0%
ATGCCGTAGATCCGAGATCG+AGG - chr3.2:66400167-66400186 MS.gene29019:CDS 55.0%
CAACCTTCGCCCATTCCAAG+TGG + chr3.2:66400823-66400842 None:intergenic 55.0%
CACTTGGAATGGGCGAAGGT+TGG - chr3.2:66400821-66400840 MS.gene29019:CDS 55.0%
GGTCGGGCATACTGTAAGAC+AGG - chr3.2:66400490-66400509 MS.gene29019:CDS 55.0%
GTCTGACCATGTACCTGTCG+AGG + chr3.2:66400605-66400624 None:intergenic 55.0%
TTAGTACCTGCTTGCCGTGC+GGG + chr3.2:66400847-66400866 None:intergenic 55.0%
! ATGCCACTTGGAATGGGCGA+AGG - chr3.2:66400817-66400836 MS.gene29019:CDS 55.0%
! CCCTTTTGCGGCTGTGCTAT+TGG - chr3.2:66400349-66400368 MS.gene29019:CDS 55.0%
! GGCTGTGCTATTGGAGCTGT+TGG - chr3.2:66400358-66400377 MS.gene29019:CDS 55.0%
! TGAGGATGGTGTCGAGGAAG+TGG - chr3.2:66400035-66400054 MS.gene29019:CDS 55.0%
GGCCGAGCTGCGCAGTAATT+TGG - chr3.2:66401095-66401114 MS.gene29019:CDS 60.0%
!! GGGGTTAGTCAAGGTCCAGC+TGG - chr3.2:66400544-66400563 MS.gene29019:CDS 60.0%
ACTGCGTCTGTTTCGGCCGG+CGG + chr3.2:66401183-66401202 None:intergenic 65.0%
CTGCGTCTGTTTCGGCCGGC+GGG + chr3.2:66401182-66401201 None:intergenic 70.0%
! TGGGCGAAGGTTGGCCCGCA+CGG - chr3.2:66400830-66400849 MS.gene29019:CDS 70.0%
! GGTTGGCCCGCACGGCAAGC+AGG - chr3.2:66400838-66400857 MS.gene29019:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 66399836 66401260 66399836 ID=MS.gene29019
chr3.2 mRNA 66399836 66401260 66399836 ID=MS.gene29019.t1;Parent=MS.gene29019
chr3.2 exon 66399836 66401260 66399836 ID=MS.gene29019.t1.exon1;Parent=MS.gene29019.t1
chr3.2 CDS 66399836 66401260 66399836 ID=cds.MS.gene29019.t1;Parent=MS.gene29019.t1
Gene Sequence

>MS.gene29019

ATGGGTTCGTTTTTCAGTGTGGCGAAGAATACTAATTCGGATCAAAGTGAGTGGGATGGAGTCTCTTTGAATGATACATGTAAGAGGCAAAGAATGTCGCATACCGTTGATGAAGAGAATCCAAGACTGTTTCCAAATCTCCCTGATGAGGTATCGATTCAGATTATTGCTAGACTTCCGAGAATTTGTTACTTCAATGTGAGGATGGTGTCGAGGAAGTGGAAGTCGACTGTCACGAGTTCGGAACTATATAAAGTAAGAAAAGAACTAGGGACGACGGAAGAGTGGCTGTATTTGTTGGTTAAGTTGGGAGAAAATAAGCTTTTGTGGCATGCCGTAGATCCGAGATCGAGGATATGGCAGAGATTGCCTACTATGCCTAATGTTGTTGATGAAGAAGAATCTAAAAAGGGTTCATCTTCTAGGCTTTGGATGTGGAACATGGTGGAAGGTATTAGAATTGCTGAAGTTATTAGAGGCTTTCTTGGAAAGAGGGATGCATTTGATGAAATGCCCTTTTGCGGCTGTGCTATTGGAGCTGTTGGTGGATGTCTCTATGTTTTAGGTGGTTTCTCTAAAGCTTCAACTATGAAATGTGTCTGGCGATTTGATCCGATGCAGAATACATGGAGCAAGGTGACTTCTATGTCTATTGGTCGGGCATACTGTAAGACAGGGATCTTAAATAACAAGCTTTATGTTGTTGGAGGGGTTAGTCAAGGTCCAGCTGGATTGATACCTCTTCAATCTGCTGAAGTTTTTGATCCCTCGACAGGTACATGGTCAGACGTGCCTAGTATGCCGTTTTCGAGAGCTCAGGTTCTTCCAAATGCTTTCTTGGCCGACATGTTAAAGCCGATTGCCACAGGTTTGACAGCATACATGGGAAGGTTATGCGTTCCACAAAGTTTATATTCATGGCCCTTTTTTGTTGATGTTGGAGGAGAAATCTATGATCCTGAAACAAACTCATGGATAGAAATGCCACTTGGAATGGGCGAAGGTTGGCCCGCACGGCAAGCAGGTACTAAATTGAGTGTCGTAGTCGATGGCGAATTATACGCTTTTGATCCTTCCAATACTATGGACAGTGGAAGGATCAAGGTATATGATCAAGGAGAAGATGCTTGGAAAGTTGTCATTGGAAAAGTCCCTATATATGGTTCTGATGATTCAGAGTCTCCTTATCTACTTGCTGGTTTCCATGGAAAACTTCATGTCATCACAAAAGATGCTGATCATAACATTGCTGTTCTGCAGGCCGAGCTGCGCAGTAATTTGGATTCTTCACCATCATCACCCTCATCAACACTGTCACAAAGTTCCTTACAAGAATTTGCGGACCCGCCGGCCGAAACAGACGCAGTTGTTTGGAAGATAGTTGCTAGTAGGTGTTTGGGACAGGCTGAACTAGTTAGTTGCCAA
Protein sequence

>MS.gene29019.t1

MGSFFSVAKNTNSDQSEWDGVSLNDTCKRQRMSHTVDEENPRLFPNLPDEVSIQIIARLPRICYFNVRMVSRKWKSTVTSSELYKVRKELGTTEEWLYLLVKLGENKLLWHAVDPRSRIWQRLPTMPNVVDEEESKKGSSSRLWMWNMVEGIRIAEVIRGFLGKRDAFDEMPFCGCAIGAVGGCLYVLGGFSKASTMKCVWRFDPMQNTWSKVTSMSIGRAYCKTGILNNKLYVVGGVSQGPAGLIPLQSAEVFDPSTGTWSDVPSMPFSRAQVLPNAFLADMLKPIATGLTAYMGRLCVPQSLYSWPFFVDVGGEIYDPETNSWIEMPLGMGEGWPARQAGTKLSVVVDGELYAFDPSNTMDSGRIKVYDQGEDAWKVVIGKVPIYGSDDSESPYLLAGFHGKLHVITKDADHNIAVLQAELRSNLDSSPSSPSSTLSQSSLQEFADPPAETDAVVWKIVASRCLGQAELVSCQ