Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29789.t1 | XP_003602090.2 | 98.2 | 56 | 1 | 0 | 1 | 56 | 58 | 113 | 8.30E-25 | 122.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29789.t1 | Q84W56 | 89.3 | 56 | 6 | 0 | 1 | 56 | 90 | 145 | 4.1e-25 | 114.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29789.t1 | G7J5I7 | 98.2 | 56 | 1 | 0 | 1 | 56 | 58 | 113 | 6.0e-25 | 122.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055539 | MS.gene29789 | 0.817832 | 2.69E-52 | -1.69E-46 |
| MS.gene061231 | MS.gene29789 | 0.825209 | 5.31E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29789.t1 | MTR_3g089020 | 98.214 | 56 | 1 | 0 | 1 | 56 | 58 | 113 | 2.73e-36 | 127 |
| MS.gene29789.t1 | MTR_4g103770 | 92.727 | 55 | 4 | 0 | 1 | 55 | 62 | 116 | 1.75e-34 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29789.t1 | AT5G63420 | 89.286 | 56 | 6 | 0 | 1 | 56 | 90 | 145 | 3.29e-33 | 119 |
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTGGTTTGGGTGAAAT+TGG | 0.278425 | 3.3:-64657278 | MS.gene29789:CDS |
| CCATTACGTGTTCTTCCTAT+TGG | 0.296621 | 3.3:-64657296 | MS.gene29789:CDS |
| TGTTCTTCCTATTGGTGGTT+TGG | 0.367711 | 3.3:-64657288 | MS.gene29789:CDS |
| GTTCTTCCTATTGGTGGTTT+GGG | 0.382494 | 3.3:-64657287 | MS.gene29789:CDS |
| GAATGAATTGTATGCTTGTT+GGG | 0.394201 | 3.3:-64657256 | MS.gene29789:CDS |
| GGAATGAATTGTATGCTTGT+TGG | 0.453098 | 3.3:-64657257 | MS.gene29789:CDS |
| TTACGTGTTCTTCCTATTGG+TGG | 0.453954 | 3.3:-64657293 | MS.gene29789:CDS |
| ATTTCACCCAAACCACCAAT+AGG | 0.461698 | 3.3:+64657281 | None:intergenic |
| ACTATTAAGGACCACTTACT+CGG | 0.483080 | 3.3:+64657190 | None:intergenic |
| CGTTATATCCTCATTGATGC+TGG | 0.516389 | 3.3:-64657224 | MS.gene29789:CDS |
| CAGCATCAATGAGGATATAA+CGG | 0.531872 | 3.3:+64657225 | None:intergenic |
| AGATTCTGTTCAACGTAGGA+TGG | 0.542555 | 3.3:-64657348 | MS.gene29789:CDS |
| CTATTAAGGACCACTTACTC+GGG | 0.570977 | 3.3:+64657191 | None:intergenic |
| CCAATAGGAAGAACACGTAA+TGG | 0.600542 | 3.3:+64657296 | None:intergenic |
| ACATGATACCAGCATCAATG+AGG | 0.660800 | 3.3:+64657216 | None:intergenic |
| TGGAAGATTCTGTTCAACGT+AGG | 0.664297 | 3.3:-64657352 | MS.gene29789:CDS |
| ATAGGAAGAACACGTAATGG+CGG | 0.726743 | 3.3:+64657299 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAATGAATTGTATGCTTGTT+GGG | - | chr3.3:64657302-64657321 | MS.gene29789:CDS | 30.0% | |
| ! | CAATTTTATGAAGGAAACGA+CGG | - | chr3.3:64657235-64657254 | MS.gene29789:CDS | 30.0% |
| CAGCATCAATGAGGATATAA+CGG | + | chr3.3:64657336-64657355 | None:intergenic | 35.0% | |
| GGAATGAATTGTATGCTTGT+TGG | - | chr3.3:64657301-64657320 | MS.gene29789:CDS | 35.0% | |
| ! | AGGATGGAGCAATTTTATGA+AGG | - | chr3.3:64657226-64657245 | MS.gene29789:CDS | 35.0% |
| ACATGATACCAGCATCAATG+AGG | + | chr3.3:64657345-64657364 | None:intergenic | 40.0% | |
| AGATTCTGTTCAACGTAGGA+TGG | - | chr3.3:64657210-64657229 | MS.gene29789:CDS | 40.0% | |
| ATAGGAAGAACACGTAATGG+CGG | + | chr3.3:64657262-64657281 | None:intergenic | 40.0% | |
| ATTTCACCCAAACCACCAAT+AGG | + | chr3.3:64657280-64657299 | None:intergenic | 40.0% | |
| CCAATAGGAAGAACACGTAA+TGG | + | chr3.3:64657265-64657284 | None:intergenic | 40.0% | |
| CCATTACGTGTTCTTCCTAT+TGG | - | chr3.3:64657262-64657281 | MS.gene29789:CDS | 40.0% | |
| CGTTATATCCTCATTGATGC+TGG | - | chr3.3:64657334-64657353 | MS.gene29789:CDS | 40.0% | |
| TGGAAGATTCTGTTCAACGT+AGG | - | chr3.3:64657206-64657225 | MS.gene29789:CDS | 40.0% | |
| TTACGTGTTCTTCCTATTGG+TGG | - | chr3.3:64657265-64657284 | MS.gene29789:CDS | 40.0% | |
| ! | ATTGGTGGTTTGGGTGAAAT+TGG | - | chr3.3:64657280-64657299 | MS.gene29789:CDS | 40.0% |
| ! | GTTCTTCCTATTGGTGGTTT+GGG | - | chr3.3:64657271-64657290 | MS.gene29789:CDS | 40.0% |
| ! | TGTTCTTCCTATTGGTGGTT+TGG | - | chr3.3:64657270-64657289 | MS.gene29789:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 64657205 | 64657375 | 64657205 | ID=MS.gene29789 |
| chr3.3 | mRNA | 64657205 | 64657375 | 64657205 | ID=MS.gene29789.t1;Parent=MS.gene29789 |
| chr3.3 | exon | 64657205 | 64657375 | 64657205 | ID=MS.gene29789.t1.exon1;Parent=MS.gene29789.t1 |
| chr3.3 | CDS | 64657205 | 64657375 | 64657205 | ID=cds.MS.gene29789.t1;Parent=MS.gene29789.t1 |
| Gene Sequence |
| Protein sequence |
