Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30005.t1 | KEH22222.1 | 99.8 | 530 | 1 | 0 | 1 | 530 | 1 | 530 | 0.00E+00 | 1078.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30005.t1 | F4J117 | 67.9 | 529 | 165 | 4 | 4 | 530 | 65 | 590 | 2.8e-216 | 752.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30005.t1 | A0A072TXQ6 | 99.8 | 530 | 1 | 0 | 1 | 530 | 1 | 530 | 0.0e+00 | 1078.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056609 | MS.gene30005 | 0.80059 | 1.34E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30005.t1 | MTR_7g034465 | 99.811 | 530 | 1 | 0 | 1 | 530 | 1 | 530 | 0.0 | 1109 |
| MS.gene30005.t1 | MTR_7g034465 | 98.670 | 451 | 6 | 0 | 1 | 451 | 107 | 557 | 0.0 | 933 |
| MS.gene30005.t1 | MTR_1g090867 | 32.246 | 276 | 167 | 10 | 222 | 489 | 94 | 357 | 7.38e-35 | 135 |
| MS.gene30005.t1 | MTR_3g094300 | 39.773 | 176 | 104 | 2 | 208 | 381 | 72 | 247 | 1.44e-31 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30005.t1 | AT3G01510 | 67.235 | 528 | 170 | 3 | 4 | 530 | 65 | 590 | 0.0 | 751 |
| MS.gene30005.t1 | AT3G01510 | 66.222 | 450 | 149 | 3 | 82 | 530 | 1 | 448 | 0.0 | 630 |
| MS.gene30005.t1 | AT3G01510 | 66.222 | 450 | 149 | 3 | 82 | 530 | 1 | 448 | 0.0 | 630 |
| MS.gene30005.t1 | AT3G52180 | 31.518 | 257 | 163 | 7 | 209 | 461 | 74 | 321 | 2.44e-34 | 134 |
| MS.gene30005.t1 | AT3G52180 | 31.064 | 235 | 150 | 6 | 230 | 461 | 1 | 226 | 6.32e-31 | 122 |
| MS.gene30005.t1 | AT3G52180 | 35.593 | 177 | 110 | 4 | 209 | 382 | 74 | 249 | 3.27e-28 | 114 |
| MS.gene30005.t1 | AT3G52180 | 35.593 | 177 | 110 | 4 | 209 | 382 | 74 | 249 | 5.20e-28 | 114 |
| MS.gene30005.t1 | AT3G10940 | 34.091 | 176 | 114 | 2 | 208 | 381 | 68 | 243 | 1.85e-27 | 112 |
Find 123 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATATTATGTACAACTATT+TGG | 0.156659 | 7.1:+61906906 | None:intergenic |
| TACTACAAGTTCATTGTTAA+TGG | 0.178065 | 7.1:-61907257 | MS.gene30005:CDS |
| TAGTGGTTCTTTCCAATTTC+CGG | 0.188722 | 7.1:+61907524 | None:intergenic |
| GTGGAACTGAAGCTGAAAAT+TGG | 0.234882 | 7.1:-61912996 | MS.gene30005:CDS |
| AATAAAAGCCCAACACAAAA+TGG | 0.242291 | 7.1:+61912490 | None:intergenic |
| TTGGAGACTTTACCGGAAAT+TGG | 0.250275 | 7.1:-61907536 | MS.gene30005:CDS |
| CCCCGATGCTTTGCCTTTAG+TGG | 0.262670 | 7.1:+61907507 | None:intergenic |
| AGTGATAGCTGTGGGAATTC+TGG | 0.278980 | 7.1:-61914022 | MS.gene30005:CDS |
| CCCTACATGCAGCCCATACT+TGG | 0.290737 | 7.1:-61912363 | MS.gene30005:CDS |
| TATATTATGTACAACTATTT+GGG | 0.331592 | 7.1:+61906907 | None:intergenic |
| CGGTTTGCTCTTACTTCTGA+TGG | 0.338438 | 7.1:-61916151 | MS.gene30005:CDS |
| TCCACTTGAGGAATATATTC+AGG | 0.341950 | 7.1:-61913822 | MS.gene30005:CDS |
| GCATCCCAGTGACCCAAGTA+TGG | 0.349243 | 7.1:+61912350 | None:intergenic |
| TTGATAAGCCTCTTGGTATT+CGG | 0.362873 | 7.1:-61916171 | MS.gene30005:CDS |
| TGGAACTGAAGCTGAAAATT+GGG | 0.363423 | 7.1:-61912995 | MS.gene30005:CDS |
| TTTCCACATCATCTCCTGTT+TGG | 0.364108 | 7.1:+61913587 | None:intergenic |
| CATGTTGCCCATGCAATTGC+CGG | 0.376920 | 7.1:+61907899 | None:intergenic |
| TCAAAGTTTCTTAATTCAAA+TGG | 0.388446 | 7.1:-61913983 | MS.gene30005:CDS |
| ACCACTAAAGGCAAAGCATC+GGG | 0.395885 | 7.1:-61907508 | MS.gene30005:CDS |
| CTTATTATGCAGCAGTTGAA+TGG | 0.397899 | 7.1:+61914135 | None:intergenic |
| GTTAACAACATAATTATGAT+TGG | 0.402614 | 7.1:-61907182 | MS.gene30005:CDS |
| TCATCATCAACTATCCTATA+CGG | 0.410245 | 7.1:-61912924 | MS.gene30005:intron |
| GAGTAACAAGATACTCATTC+AGG | 0.410665 | 7.1:+61916194 | None:intergenic |
| GTCTTGTTCCATGTGACAAC+AGG | 0.413498 | 7.1:+61914073 | None:intergenic |
| AAGCGAGTAGAGATGGAGAT+TGG | 0.415212 | 7.1:-61913859 | MS.gene30005:CDS |
| TGGACCCTAGATTACCGTAT+AGG | 0.418003 | 7.1:+61912910 | None:intergenic |
| GTTACTCTTGATAAGCCTCT+TGG | 0.426192 | 7.1:-61916178 | MS.gene30005:CDS |
| CGGCAATTGCATGGGCAACA+TGG | 0.433325 | 7.1:-61907898 | MS.gene30005:CDS |
| ATCATCTCCTGTTTGGATGC+AGG | 0.436675 | 7.1:+61913594 | None:intergenic |
| AATTTGGTTGGAGACTTTAC+CGG | 0.441864 | 7.1:-61907543 | MS.gene30005:CDS |
| CCAAGTATGGGCTGCATGTA+GGG | 0.447468 | 7.1:+61912363 | None:intergenic |
| GAATGCTTTACTAACCCCTT+TGG | 0.450789 | 7.1:+61916105 | None:intergenic |
| GCAGCCCATACTTGGGTCAC+TGG | 0.460968 | 7.1:-61912355 | MS.gene30005:CDS |
| GTGGGATCCTGCATCCAAAC+AGG | 0.468080 | 7.1:-61913601 | MS.gene30005:CDS |
| AACATCTTCACCAGCTGAAA+GGG | 0.471958 | 7.1:-61907219 | MS.gene30005:CDS |
| TTCAATCACCTTTACAACAT+TGG | 0.473932 | 7.1:+61907014 | None:intergenic |
| TGCACACGTGCAGGCCTGAC+AGG | 0.474713 | 7.1:-61912330 | MS.gene30005:intron |
| TTTCCTCAGGAAGGTGCTAA+AGG | 0.475366 | 7.1:-61914209 | MS.gene30005:intron |
| TTCCCACAGCTATCACTAGA+TGG | 0.476938 | 7.1:+61914028 | None:intergenic |
| CCGCTATCATCCCTTTCAGC+TGG | 0.477134 | 7.1:+61907209 | None:intergenic |
| ACTGCAGGCCGGCAATTGCA+TGG | 0.478258 | 7.1:-61907907 | MS.gene30005:intron |
| TCTTGGTATGCGTTATAGTA+AGG | 0.480275 | 7.1:-61913759 | MS.gene30005:intron |
| TTGTCCACTCTCTCGCCAAA+GGG | 0.485813 | 7.1:-61916120 | MS.gene30005:CDS |
| AGCGAGTAGAGATGGAGATT+GGG | 0.487159 | 7.1:-61913858 | MS.gene30005:CDS |
| TTTCAGCTTCAGTTCCACTT+TGG | 0.489822 | 7.1:+61913001 | None:intergenic |
| GATTACCGAGCAAATATATG+TGG | 0.494561 | 7.1:-61913620 | MS.gene30005:CDS |
| CCCAAGTATGGGCTGCATGT+AGG | 0.497954 | 7.1:+61912362 | None:intergenic |
| TTATTATGCAGCAGTTGAAT+GGG | 0.498131 | 7.1:+61914136 | None:intergenic |
| ATAAAAGCCCAACACAAAAT+GGG | 0.499871 | 7.1:+61912491 | None:intergenic |
| AGAGATGGAGATTGGGCTCA+TGG | 0.504002 | 7.1:-61913851 | MS.gene30005:CDS |
| ATTTCTTTCACCGTGGGTGA+TGG | 0.504844 | 7.1:+61913928 | None:intergenic |
| GGGGAGGATGTAAATTTGGT+TGG | 0.505086 | 7.1:-61907555 | MS.gene30005:CDS |
| GATCTTATAGCTATGGTTGA+AGG | 0.508873 | 7.1:-61907875 | MS.gene30005:CDS |
| AGGGTAACGCGGAGAAGTCC+AGG | 0.513501 | 7.1:-61915310 | MS.gene30005:CDS |
| CTTGCACTTCTGGATTTGAT+CGG | 0.516485 | 7.1:-61912432 | MS.gene30005:CDS |
| CAGCCCATACTTGGGTCACT+GGG | 0.519078 | 7.1:-61912354 | MS.gene30005:CDS |
| GGCAATTGCATGGGCAACAT+GGG | 0.519858 | 7.1:-61907897 | MS.gene30005:CDS |
| GCCTGAATATATTCCTCAAG+TGG | 0.522255 | 7.1:+61913821 | None:intergenic |
| CTGCAGGCCGGCAATTGCAT+GGG | 0.526196 | 7.1:-61907906 | MS.gene30005:intron |
| CTTGAAATAGTATATAATAG+AGG | 0.527177 | 7.1:-61914103 | MS.gene30005:CDS |
| TATTTGGGAGCTAATCTGAC+AGG | 0.531178 | 7.1:+61906922 | None:intergenic |
| TCACTGGGATGCACACGTGC+AGG | 0.531651 | 7.1:-61912339 | MS.gene30005:CDS |
| ATTATAGTACATCTCATCCT+TGG | 0.534194 | 7.1:+61913787 | None:intergenic |
| TTTAGCAAGAATAACCTGTC+AGG | 0.534696 | 7.1:+61912316 | None:intergenic |
| CAACATCTTCACCAGCTGAA+AGG | 0.536122 | 7.1:-61907220 | MS.gene30005:CDS |
| GAGGTTTATGTTGGCGAAAG+CGG | 0.536500 | 7.1:-61906971 | MS.gene30005:CDS |
| ATTGTCCACTCTCTCGCCAA+AGG | 0.537036 | 7.1:-61916121 | MS.gene30005:CDS |
| AGGAGATGATGTGGAAACCT+TGG | 0.537654 | 7.1:-61913581 | MS.gene30005:CDS |
| GTCACCCACCATGATTATCC+TGG | 0.539215 | 7.1:+61915292 | None:intergenic |
| AACCACTAAAGGCAAAGCAT+CGG | 0.543234 | 7.1:-61907509 | MS.gene30005:CDS |
| ACTGACGAAGCGAGTAGAGA+TGG | 0.549177 | 7.1:-61913866 | MS.gene30005:CDS |
| ATGTACTATAATCACTCTCT+TGG | 0.549559 | 7.1:-61913776 | MS.gene30005:CDS |
| GGTTTGCTCTTACTTCTGAT+GGG | 0.552980 | 7.1:-61916150 | MS.gene30005:CDS |
| CTTGTTGAGATTAAAGACGT+TGG | 0.553051 | 7.1:-61915233 | MS.gene30005:CDS |
| AACATGGGATCTTATAGCTA+TGG | 0.554777 | 7.1:-61907882 | MS.gene30005:CDS |
| CAATCAATGACTATTGTCAA+AGG | 0.562058 | 7.1:-61912957 | MS.gene30005:CDS |
| TAACCCCTTTGGCGAGAGAG+TGG | 0.565876 | 7.1:+61916116 | None:intergenic |
| AGGTATGCAATCACACAAGC+AGG | 0.569325 | 7.1:+61912406 | None:intergenic |
| AAACATCTGTCATCCAATGT+AGG | 0.571704 | 7.1:+61912386 | None:intergenic |
| CCTACATGCAGCCCATACTT+GGG | 0.572114 | 7.1:-61912362 | MS.gene30005:CDS |
| GTCGAGTTCCTGTTGTCACA+TGG | 0.574747 | 7.1:-61914081 | MS.gene30005:CDS |
| AAATTGGAAAGAACCACTAA+AGG | 0.574783 | 7.1:-61907520 | MS.gene30005:CDS |
| AGTTCATTGTTAATGGACAA+TGG | 0.577080 | 7.1:-61907250 | MS.gene30005:CDS |
| TGAATGGGAAATGTAGATGC+GGG | 0.577092 | 7.1:+61914151 | None:intergenic |
| GGAAACCTTGGCAAAAGTTG+AGG | 0.590873 | 7.1:-61913569 | MS.gene30005:intron |
| GGAGAAGTCCAGGATAATCA+TGG | 0.591350 | 7.1:-61915300 | MS.gene30005:CDS |
| GGTGACACTTTGAAGAAAGC+TGG | 0.592970 | 7.1:-61915275 | MS.gene30005:CDS |
| CATCCAAACAGGAGATGATG+TGG | 0.600453 | 7.1:-61913590 | MS.gene30005:CDS |
| CACAAATGTGACAGCATGTG+TGG | 0.600778 | 7.1:+61907835 | None:intergenic |
| GTAGGATGCCAATGTTGTAA+AGG | 0.602731 | 7.1:-61907022 | MS.gene30005:intron |
| TAACGAATTTCTTTCACCGT+GGG | 0.604263 | 7.1:+61913922 | None:intergenic |
| GGATCCCACATATATTTGCT+CGG | 0.608187 | 7.1:+61913615 | None:intergenic |
| GCTGTCACATTTGTGTGGAA+TGG | 0.617368 | 7.1:-61907827 | MS.gene30005:CDS |
| AACCATCTAGTGATAGCTGT+GGG | 0.620367 | 7.1:-61914030 | MS.gene30005:CDS |
| GCTGTTCTGAACTTCCAAAG+TGG | 0.625552 | 7.1:-61913015 | MS.gene30005:CDS |
| CACATGCTGTCACATTTGTG+TGG | 0.626265 | 7.1:-61907832 | MS.gene30005:CDS |
| CATCCCAGTGACCCAAGTAT+GGG | 0.627109 | 7.1:+61912351 | None:intergenic |
| ATGGAGATTCTTATGACATG+AGG | 0.627266 | 7.1:-61912522 | MS.gene30005:CDS |
| TTGAATGGGAAATGTAGATG+CGG | 0.634352 | 7.1:+61914150 | None:intergenic |
| GGAACTGAAGCTGAAAATTG+GGG | 0.634841 | 7.1:-61912994 | MS.gene30005:CDS |
| CAACCATCTAGTGATAGCTG+TGG | 0.635422 | 7.1:-61914031 | MS.gene30005:CDS |
| CTTATAGCTATGGTTGAAGG+TGG | 0.641208 | 7.1:-61907872 | MS.gene30005:CDS |
| CTGAACAGAAGGACGAACGC+TGG | 0.641353 | 7.1:+61907151 | None:intergenic |
| ATTACCGAGCAAATATATGT+GGG | 0.647709 | 7.1:-61913619 | MS.gene30005:CDS |
| GTAGAAGTTAAGCTTCCGCA+AGG | 0.658658 | 7.1:-61907468 | MS.gene30005:CDS |
| AATCAACATGCCATCACCCA+CGG | 0.660027 | 7.1:-61913938 | MS.gene30005:CDS |
| GTGTGATTGCATACCTACAT+TGG | 0.665139 | 7.1:-61912399 | MS.gene30005:CDS |
| TAGCACACATACTTTCCTTG+CGG | 0.665443 | 7.1:+61907453 | None:intergenic |
| AAGTCCAGGATAATCATGGT+GGG | 0.669720 | 7.1:-61915296 | MS.gene30005:CDS |
| TCAGGCTCTAGATCGTGCCA+AGG | 0.672514 | 7.1:-61913804 | MS.gene30005:CDS |
| ATTTGGGAGCTAATCTGACA+GGG | 0.675948 | 7.1:+61906923 | None:intergenic |
| GAAGTCCAGGATAATCATGG+TGG | 0.676899 | 7.1:-61915297 | MS.gene30005:CDS |
| ACTTTGGAAGTTCAGAACAG+CGG | 0.681247 | 7.1:+61913017 | None:intergenic |
| CTAAAGGCAAAGCATCGGGG+TGG | 0.681617 | 7.1:-61907504 | MS.gene30005:CDS |
| TGTCCACTCTCTCGCCAAAG+GGG | 0.683682 | 7.1:-61916119 | MS.gene30005:intron |
| CTCAACAAGACTATTCTGAG+AGG | 0.688346 | 7.1:+61915247 | None:intergenic |
| AGAGCAAACCGAATACCAAG+AGG | 0.690168 | 7.1:+61916163 | None:intergenic |
| CATGTGTGGGAGCATCATGT+TGG | 0.696530 | 7.1:+61907849 | None:intergenic |
| CCAGCTGAAAGGGATGATAG+CGG | 0.703831 | 7.1:-61907209 | MS.gene30005:CDS |
| TTAACGAATTTCTTTCACCG+TGG | 0.706941 | 7.1:+61913921 | None:intergenic |
| CCACTAAAGGCAAAGCATCG+GGG | 0.708033 | 7.1:-61907507 | MS.gene30005:CDS |
| ATTTGTGTGGAATGGTCATG+AGG | 0.717145 | 7.1:-61907819 | MS.gene30005:intron |
| ACAAATGTGACAGCATGTGT+GGG | 0.717710 | 7.1:+61907836 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 61906921 | 61916251 | 61906921 | ID=MS.gene30005 |
| chr7.1 | mRNA | 61906921 | 61916251 | 61906921 | ID=MS.gene30005.t1;Parent=MS.gene30005 |
| chr7.1 | exon | 61916120 | 61916251 | 61916120 | ID=MS.gene30005.t1.exon1;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61916120 | 61916251 | 61916120 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61915224 | 61915330 | 61915224 | ID=MS.gene30005.t1.exon2;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61915224 | 61915330 | 61915224 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61913760 | 61914222 | 61913760 | ID=MS.gene30005.t1.exon3;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61913760 | 61914222 | 61913760 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61913570 | 61913641 | 61913570 | ID=MS.gene30005.t1.exon4;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61913570 | 61913641 | 61913570 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61912925 | 61913046 | 61912925 | ID=MS.gene30005.t1.exon5;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61912925 | 61913046 | 61912925 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61912331 | 61912546 | 61912331 | ID=MS.gene30005.t1.exon6;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61912331 | 61912546 | 61912331 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61907820 | 61907922 | 61907820 | ID=MS.gene30005.t1.exon7;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61907820 | 61907922 | 61907820 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61907465 | 61907577 | 61907465 | ID=MS.gene30005.t1.exon8;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61907465 | 61907577 | 61907465 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61907139 | 61907283 | 61907139 | ID=MS.gene30005.t1.exon9;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61907139 | 61907283 | 61907139 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| chr7.1 | exon | 61906921 | 61907040 | 61906921 | ID=MS.gene30005.t1.exon10;Parent=MS.gene30005.t1 |
| chr7.1 | CDS | 61906921 | 61907040 | 61906921 | ID=cds.MS.gene30005.t1;Parent=MS.gene30005.t1 |
| Gene Sequence |
| Protein sequence |
