Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30083.t1 | KEH22154.1 | 94.5 | 127 | 7 | 0 | 23 | 149 | 150 | 276 | 4.20E-60 | 240.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30083.t1 | A0A072TXC7 | 94.5 | 127 | 7 | 0 | 23 | 149 | 150 | 276 | 3.1e-60 | 240.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058279 | MS.gene30083 | 0.80982 | 1.57E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30083.t1 | MTR_7g033720 | 94.488 | 127 | 7 | 0 | 23 | 149 | 150 | 276 | 8.88e-81 | 243 |
| MS.gene30083.t1 | MTR_7g033720 | 95.200 | 125 | 6 | 0 | 25 | 149 | 219 | 343 | 6.54e-79 | 241 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30083.t1 | AT5G40470 | 52.800 | 125 | 56 | 2 | 26 | 149 | 251 | 373 | 9.63e-33 | 120 |
Find 28 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCGGAGATGATTATGGAAT+TGG | 0.274216 | 7.1:-63901722 | MS.gene30083:intron |
| TAACTCATTGGAATCACTTT+TGG | 0.292711 | 7.1:-63901528 | MS.gene30083:CDS |
| GGTATTCGCGGAGATGATTA+TGG | 0.301026 | 7.1:-63901728 | MS.gene30083:CDS |
| CAAAGTTGTTGTCTTGTAAC+TGG | 0.316411 | 7.1:-63901341 | MS.gene30083:CDS |
| GATGTTGTTGAAAGGGAAAA+AGG | 0.374834 | 7.1:-63901251 | MS.gene30083:intron |
| TTCGAACCCTTTCGAGTCTT+AGG | 0.375285 | 7.1:-63901367 | MS.gene30083:CDS |
| TGCACAACATTGTAACTCAT+TGG | 0.379704 | 7.1:-63901540 | MS.gene30083:CDS |
| TGTAACTGGTGAAGGGCTTA+AGG | 0.412343 | 7.1:-63901327 | MS.gene30083:CDS |
| TTGGTTGATTTGAAGGTTAG+AGG | 0.412803 | 7.1:-63901116 | None:intergenic |
| CCAATTCCTTATCAAAATCA+TGG | 0.425120 | 7.1:+63901774 | None:intergenic |
| ACTTGTAGAAGCGTGGTTGA+TGG | 0.438534 | 7.1:-63901578 | MS.gene30083:CDS |
| GAGTAATGGTCTTGAAGAAT+TGG | 0.438870 | 7.1:-63901288 | MS.gene30083:CDS |
| CTTTGTTTCGAGGGTATTCG+CGG | 0.480409 | 7.1:-63901740 | MS.gene30083:CDS |
| TGTTGTCTTGTAACTGGTGA+AGG | 0.496531 | 7.1:-63901335 | MS.gene30083:CDS |
| TTGAAGCCTAAGACTCGAAA+GGG | 0.498097 | 7.1:+63901361 | None:intergenic |
| TTGGAGAGTCTTTGTTTCGA+GGG | 0.512836 | 7.1:-63901749 | MS.gene30083:CDS |
| CTAAGTGTGGCGGTGAGTAA+TGG | 0.515597 | 7.1:-63901302 | MS.gene30083:CDS |
| CATGATGGTGGTAGCAGAGA+GGG | 0.526886 | 7.1:-63901503 | MS.gene30083:CDS |
| GTTGTCTTGTAACTGGTGAA+GGG | 0.535998 | 7.1:-63901334 | MS.gene30083:CDS |
| TTTGGAGAGTCTTTGTTTCG+AGG | 0.543255 | 7.1:-63901750 | MS.gene30083:CDS |
| AAACTGTGATGTTGTTGAAA+GGG | 0.550447 | 7.1:-63901258 | MS.gene30083:CDS |
| TTTGAAGCCTAAGACTCGAA+AGG | 0.575658 | 7.1:+63901360 | None:intergenic |
| ATGAGTTACAATGTTGTGCA+AGG | 0.576775 | 7.1:+63901543 | None:intergenic |
| TAAACTGTGATGTTGTTGAA+AGG | 0.581500 | 7.1:-63901259 | MS.gene30083:CDS |
| GCTCAAAACTTGTAGAAGCG+TGG | 0.644715 | 7.1:-63901585 | MS.gene30083:CDS |
| GCTTAAGGCTCTAAGTGTGG+CGG | 0.680396 | 7.1:-63901312 | MS.gene30083:CDS |
| AGGGCTTAAGGCTCTAAGTG+TGG | 0.683148 | 7.1:-63901315 | MS.gene30083:CDS |
| TCATGATGGTGGTAGCAGAG+AGG | 0.685433 | 7.1:-63901504 | MS.gene30083:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATGAAATGTTGTTTGATA+AGG | - | chr7.1:63901720-63901739 | MS.gene30083:intron | 20.0% |
| ! | TAAGAAGCTTCAATTTCTTA+AGG | + | chr7.1:63901219-63901238 | None:intergenic | 25.0% |
| !! | CAAATAGATGATTGTTTTCA+AGG | + | chr7.1:63901483-63901502 | None:intergenic | 25.0% |
| !!! | TATTCATTTGGTTGATTTGA+AGG | - | chr7.1:63901768-63901787 | MS.gene30083:CDS | 25.0% |
| AAACTGTGATGTTGTTGAAA+GGG | - | chr7.1:63901633-63901652 | MS.gene30083:intron | 30.0% | |
| TAAACTGTGATGTTGTTGAA+AGG | - | chr7.1:63901632-63901651 | MS.gene30083:intron | 30.0% | |
| ! | ACTTGATTTTCGTTTACCTA+TGG | - | chr7.1:63901456-63901475 | MS.gene30083:CDS | 30.0% |
| ! | ATTATGGAATTGGTTGGTTA+TGG | - | chr7.1:63901179-63901198 | MS.gene30083:intron | 30.0% |
| ! | GTTGGTTTCATGTATTCATT+TGG | - | chr7.1:63901756-63901775 | MS.gene30083:CDS | 30.0% |
| ! | TAACTCATTGGAATCACTTT+TGG | - | chr7.1:63901363-63901382 | MS.gene30083:CDS | 30.0% |
| ! | TAAGGATTTTGTGTCAATGT+TGG | - | chr7.1:63901738-63901757 | MS.gene30083:CDS | 30.0% |
| ! | TCTTTTGTTCAATGTTTGCA+GGG | - | chr7.1:63901268-63901287 | MS.gene30083:CDS | 30.0% |
| ! | TTCTTTTGTTCAATGTTTGC+AGG | - | chr7.1:63901267-63901286 | MS.gene30083:CDS | 30.0% |
| ! | TTTTGATAAGGAATTGGGTT+TGG | - | chr7.1:63901123-63901142 | MS.gene30083:intron | 30.0% |
| ATGAGTTACAATGTTGTGCA+AGG | + | chr7.1:63901351-63901370 | None:intergenic | 35.0% | |
| CAAAGTTGTTGTCTTGTAAC+TGG | - | chr7.1:63901550-63901569 | MS.gene30083:CDS | 35.0% | |
| CTTCTTAGTTGTCAAGGAAT+TGG | - | chr7.1:63901232-63901251 | MS.gene30083:intron | 35.0% | |
| GAGATGATTATGGAATTGGT+TGG | - | chr7.1:63901173-63901192 | MS.gene30083:intron | 35.0% | |
| GATGTTGTTGAAAGGGAAAA+AGG | - | chr7.1:63901640-63901659 | MS.gene30083:intron | 35.0% | |
| TGCACAACATTGTAACTCAT+TGG | - | chr7.1:63901351-63901370 | MS.gene30083:CDS | 35.0% | |
| TTGAAGCTTCTTAGTTGTCA+AGG | - | chr7.1:63901226-63901245 | MS.gene30083:intron | 35.0% | |
| ! | GAGTAATGGTCTTGAAGAAT+TGG | - | chr7.1:63901603-63901622 | MS.gene30083:CDS | 35.0% |
| !! | AAAGGTTTGCTTGCTACTTT+AGG | - | chr7.1:63901658-63901677 | MS.gene30083:intron | 35.0% |
| !! | AAGGTTTGCTTGCTACTTTA+GGG | - | chr7.1:63901659-63901678 | MS.gene30083:intron | 35.0% |
| !! | TGATTGTTTTCAAGGTCCAT+AGG | + | chr7.1:63901475-63901494 | None:intergenic | 35.0% |
| !!! | GAATCACTTTTGGTTCATGA+TGG | - | chr7.1:63901373-63901392 | MS.gene30083:CDS | 35.0% |
| CTTAGTTGTCAAGGAATTGG+TGG | - | chr7.1:63901235-63901254 | MS.gene30083:intron | 40.0% | |
| GTTGTCTTGTAACTGGTGAA+GGG | - | chr7.1:63901557-63901576 | MS.gene30083:CDS | 40.0% | |
| TGTTGTCTTGTAACTGGTGA+AGG | - | chr7.1:63901556-63901575 | MS.gene30083:CDS | 40.0% | |
| TTGAAGCCTAAGACTCGAAA+GGG | + | chr7.1:63901533-63901552 | None:intergenic | 40.0% | |
| ! | TTGGAGAGTCTTTGTTTCGA+GGG | - | chr7.1:63901142-63901161 | MS.gene30083:intron | 40.0% |
| ! | TTTGAAGCCTAAGACTCGAA+AGG | + | chr7.1:63901534-63901553 | None:intergenic | 40.0% |
| ! | TTTGGAGAGTCTTTGTTTCG+AGG | - | chr7.1:63901141-63901160 | MS.gene30083:intron | 40.0% |
| !! | CTACTTTAGGGCAACATTTG+AGG | - | chr7.1:63901671-63901690 | MS.gene30083:intron | 40.0% |
| !!! | TCACTTTTGGTTCATGATGG+TGG | - | chr7.1:63901376-63901395 | MS.gene30083:CDS | 40.0% |
| CGCGGAGATGATTATGGAAT+TGG | - | chr7.1:63901169-63901188 | MS.gene30083:intron | 45.0% | |
| GCTCAAAACTTGTAGAAGCG+TGG | - | chr7.1:63901306-63901325 | MS.gene30083:CDS | 45.0% | |
| GGTATTCGCGGAGATGATTA+TGG | - | chr7.1:63901163-63901182 | MS.gene30083:intron | 45.0% | |
| TGTAACTGGTGAAGGGCTTA+AGG | - | chr7.1:63901564-63901583 | MS.gene30083:CDS | 45.0% | |
| ! | ACTTGTAGAAGCGTGGTTGA+TGG | - | chr7.1:63901313-63901332 | MS.gene30083:CDS | 45.0% |
| ! | TTCGAACCCTTTCGAGTCTT+AGG | - | chr7.1:63901524-63901543 | MS.gene30083:CDS | 45.0% |
| !! | CTTTGTTTCGAGGGTATTCG+CGG | - | chr7.1:63901151-63901170 | MS.gene30083:intron | 45.0% |
| CTAAGTGTGGCGGTGAGTAA+TGG | - | chr7.1:63901589-63901608 | MS.gene30083:CDS | 50.0% | |
| !! | AGGGCTTAAGGCTCTAAGTG+TGG | - | chr7.1:63901576-63901595 | MS.gene30083:CDS | 50.0% |
| !! | CATGATGGTGGTAGCAGAGA+GGG | - | chr7.1:63901388-63901407 | MS.gene30083:CDS | 50.0% |
| !! | GCTTAAGGCTCTAAGTGTGG+CGG | - | chr7.1:63901579-63901598 | MS.gene30083:CDS | 50.0% |
| !! | TCATGATGGTGGTAGCAGAG+AGG | - | chr7.1:63901387-63901406 | MS.gene30083:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 63901119 | 63901794 | 63901119 | ID=MS.gene30083 |
| chr7.1 | mRNA | 63901119 | 63901794 | 63901119 | ID=MS.gene30083.t1;Parent=MS.gene30083 |
| chr7.1 | exon | 63901723 | 63901794 | 63901723 | ID=MS.gene30083.t1.exon1;Parent=MS.gene30083.t1 |
| chr7.1 | CDS | 63901723 | 63901794 | 63901723 | ID=cds.MS.gene30083.t1;Parent=MS.gene30083.t1 |
| chr7.1 | exon | 63901252 | 63901624 | 63901252 | ID=MS.gene30083.t1.exon2;Parent=MS.gene30083.t1 |
| chr7.1 | CDS | 63901252 | 63901624 | 63901252 | ID=cds.MS.gene30083.t1;Parent=MS.gene30083.t1 |
| chr7.1 | exon | 63901119 | 63901123 | 63901119 | ID=MS.gene30083.t1.exon3;Parent=MS.gene30083.t1 |
| chr7.1 | CDS | 63901119 | 63901123 | 63901119 | ID=cds.MS.gene30083.t1;Parent=MS.gene30083.t1 |
| Gene Sequence |
| Protein sequence |
