Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30171.t1 | XP_003600087.1 | 94.8 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 4.00E-43 | 183.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30171.t1 | G7IXN7 | 94.8 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 2.9e-43 | 183.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049983 | MS.gene30171 | 0.811063 | 8.44E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30171.t1 | MTR_3g051610 | 94.845 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 3.90e-66 | 194 |
| MS.gene30171.t1 | MTR_3g051530 | 83.000 | 100 | 13 | 2 | 1 | 97 | 1 | 99 | 1.34e-55 | 167 |
| MS.gene30171.t1 | MTR_3g051570 | 81.188 | 101 | 15 | 2 | 1 | 97 | 1 | 101 | 2.44e-54 | 164 |
| MS.gene30171.t1 | MTR_3g051510 | 83.000 | 100 | 13 | 2 | 1 | 97 | 1 | 99 | 4.73e-54 | 163 |
| MS.gene30171.t1 | MTR_5g092710 | 47.312 | 93 | 38 | 3 | 1 | 93 | 1 | 82 | 6.33e-23 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30171.t1 | AT3G20340 | 33.913 | 115 | 58 | 5 | 1 | 97 | 1 | 115 | 3.74e-14 | 63.2 |
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCAAGCTCAATCAGCTTC+AGG | 0.287651 | 3.3:-42996339 | None:intergenic |
| CATTACTATGGTTCATCAAT+TGG | 0.291376 | 3.3:-42996269 | None:intergenic |
| GGCACCGGCACAGTTGCTTT+TGG | 0.315364 | 3.3:-42996145 | None:intergenic |
| CTTCACCTCAGTCGTTGCTT+TGG | 0.359740 | 3.3:-42996166 | None:intergenic |
| TCAGGGATGCTTTGAAGTGC+TGG | 0.396077 | 3.3:-42996321 | None:intergenic |
| GTGATTCGTAGCCATTACTA+TGG | 0.411614 | 3.3:-42996281 | None:intergenic |
| ACGAATCACTTCAAAGATCT+TGG | 0.412006 | 3.3:+42996295 | MS.gene30171:CDS |
| ATTACTATGGTTCATCAATT+GGG | 0.424384 | 3.3:-42996268 | None:intergenic |
| CGACGGACGACGAGGATGAT+TGG | 0.427371 | 3.3:+42996091 | MS.gene30171:CDS |
| CTCAGTCGTTGCTTTGGCAC+CGG | 0.469921 | 3.3:-42996160 | None:intergenic |
| TTCAAGCTCAATCAGCTTCA+GGG | 0.480069 | 3.3:-42996338 | None:intergenic |
| GACGGACGACGAGGATGATT+GGG | 0.480605 | 3.3:+42996092 | MS.gene30171:CDS |
| TGCATCAGAAGGTGACTCTC+CGG | 0.510614 | 3.3:+42996122 | MS.gene30171:CDS |
| AGAAGAGTTGTTAAGCAAAG+TGG | 0.524820 | 3.3:+42996212 | MS.gene30171:CDS |
| CCGGCCAAAAGCAACTGTGC+CGG | 0.538980 | 3.3:+42996141 | MS.gene30171:CDS |
| GGACATGAGGGAGTTGAGAG+TGG | 0.564983 | 3.3:+42996233 | MS.gene30171:CDS |
| CAATTGATGAACCATAGTAA+TGG | 0.594329 | 3.3:+42996270 | MS.gene30171:CDS |
| TGTTAAGCAAAGTGGACATG+AGG | 0.604541 | 3.3:+42996220 | MS.gene30171:CDS |
| GTTAAGCAAAGTGGACATGA+GGG | 0.611329 | 3.3:+42996221 | MS.gene30171:CDS |
| AAAATACGCGACGGACGACG+AGG | 0.658350 | 3.3:+42996083 | MS.gene30171:CDS |
| CGGTGCCAAAGCAACGACTG+AGG | 0.681594 | 3.3:+42996161 | MS.gene30171:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTACTATGGTTCATCAATT+GGG | - | chr3.3:42996271-42996290 | None:intergenic | 25.0% |
| CAATTGATGAACCATAGTAA+TGG | + | chr3.3:42996270-42996289 | MS.gene30171:CDS | 30.0% | |
| CATTACTATGGTTCATCAAT+TGG | - | chr3.3:42996272-42996291 | None:intergenic | 30.0% | |
| ACGAATCACTTCAAAGATCT+TGG | + | chr3.3:42996295-42996314 | MS.gene30171:CDS | 35.0% | |
| AGAAGAGTTGTTAAGCAAAG+TGG | + | chr3.3:42996212-42996231 | MS.gene30171:CDS | 35.0% | |
| GTGATTCGTAGCCATTACTA+TGG | - | chr3.3:42996284-42996303 | None:intergenic | 40.0% | |
| GTTAAGCAAAGTGGACATGA+GGG | + | chr3.3:42996221-42996240 | MS.gene30171:CDS | 40.0% | |
| TGTTAAGCAAAGTGGACATG+AGG | + | chr3.3:42996220-42996239 | MS.gene30171:CDS | 40.0% | |
| ! | GAAGCATCAAAAATACGCGA+CGG | + | chr3.3:42996074-42996093 | MS.gene30171:CDS | 40.0% |
| ! | TGGGATTTTCATGCATCAGA+AGG | + | chr3.3:42996111-42996130 | MS.gene30171:CDS | 40.0% |
| CTTCACCTCAGTCGTTGCTT+TGG | - | chr3.3:42996169-42996188 | None:intergenic | 50.0% | |
| TGCATCAGAAGGTGACTCTC+CGG | + | chr3.3:42996122-42996141 | MS.gene30171:CDS | 50.0% | |
| !! | TCAGGGATGCTTTGAAGTGC+TGG | - | chr3.3:42996324-42996343 | None:intergenic | 50.0% |
| AAAATACGCGACGGACGACG+AGG | + | chr3.3:42996083-42996102 | MS.gene30171:CDS | 55.0% | |
| GACGGACGACGAGGATGATT+GGG | + | chr3.3:42996092-42996111 | MS.gene30171:CDS | 55.0% | |
| GGACATGAGGGAGTTGAGAG+TGG | + | chr3.3:42996233-42996252 | MS.gene30171:CDS | 55.0% | |
| ! | CTCAGTCGTTGCTTTGGCAC+CGG | - | chr3.3:42996163-42996182 | None:intergenic | 55.0% |
| CCGGCCAAAAGCAACTGTGC+CGG | + | chr3.3:42996141-42996160 | MS.gene30171:CDS | 60.0% | |
| CGACGGACGACGAGGATGAT+TGG | + | chr3.3:42996091-42996110 | MS.gene30171:CDS | 60.0% | |
| ! | CGGTGCCAAAGCAACGACTG+AGG | + | chr3.3:42996161-42996180 | MS.gene30171:CDS | 60.0% |
| !!! | CCGGCACAGTTGCTTTTGGC+CGG | - | chr3.3:42996144-42996163 | None:intergenic | 60.0% |
| !!! | GGCACCGGCACAGTTGCTTT+TGG | - | chr3.3:42996148-42996167 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 42996060 | 42996353 | 42996060 | ID=MS.gene30171 |
| chr3.3 | mRNA | 42996060 | 42996353 | 42996060 | ID=MS.gene30171.t1;Parent=MS.gene30171 |
| chr3.3 | exon | 42996060 | 42996353 | 42996060 | ID=MS.gene30171.t1.exon1;Parent=MS.gene30171.t1 |
| chr3.3 | CDS | 42996060 | 42996353 | 42996060 | ID=cds.MS.gene30171.t1;Parent=MS.gene30171.t1 |
| Gene Sequence |
| Protein sequence |
