Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30629.t1 | AFK43139.1 | 93.3 | 105 | 7 | 0 | 1 | 105 | 20 | 124 | 2.00E-48 | 201.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30629.t1 | O03982 | 59.6 | 57 | 22 | 1 | 1 | 56 | 20 | 76 | 8.3e-11 | 67.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30629.t1 | I3SS97 | 93.3 | 105 | 7 | 0 | 1 | 105 | 20 | 124 | 1.4e-48 | 201.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05410 | MS.gene30629 | 0.803542 | 3.32E-49 | -1.69E-46 |
| MS.gene058526 | MS.gene30629 | 0.842972 | 1.87E-58 | -1.69E-46 |
| MS.gene058527 | MS.gene30629 | 0.838568 | 2.66E-57 | -1.69E-46 |
| MS.gene058528 | MS.gene30629 | 0.841943 | 3.50E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30629.t1 | MTR_6g049320 | 92.381 | 105 | 8 | 0 | 1 | 105 | 50 | 154 | 3.65e-60 | 181 |
| MS.gene30629.t1 | MTR_6g049280 | 91.429 | 105 | 9 | 0 | 1 | 105 | 50 | 154 | 4.17e-60 | 181 |
| MS.gene30629.t1 | MTR_6g049280 | 91.429 | 105 | 9 | 0 | 1 | 105 | 20 | 124 | 2.08e-59 | 178 |
| MS.gene30629.t1 | MTR_6g049320 | 92.381 | 105 | 8 | 0 | 1 | 105 | 20 | 124 | 2.59e-59 | 178 |
| MS.gene30629.t1 | MTR_7g451200 | 55.056 | 89 | 38 | 1 | 1 | 89 | 20 | 106 | 5.03e-29 | 101 |
| MS.gene30629.t1 | MTR_1173s0010 | 54.545 | 88 | 38 | 1 | 1 | 88 | 20 | 105 | 6.14e-28 | 98.2 |
| MS.gene30629.t1 | MTR_1g099800 | 54.310 | 116 | 41 | 2 | 1 | 104 | 20 | 135 | 6.95e-28 | 99.0 |
| MS.gene30629.t1 | MTR_7g451210 | 56.818 | 88 | 36 | 1 | 1 | 88 | 20 | 105 | 2.51e-27 | 97.1 |
| MS.gene30629.t1 | MTR_3g434890 | 55.682 | 88 | 37 | 1 | 1 | 88 | 20 | 105 | 3.68e-27 | 96.3 |
| MS.gene30629.t1 | MTR_6g051690 | 54.545 | 110 | 43 | 3 | 1 | 105 | 20 | 127 | 7.93e-27 | 95.9 |
| MS.gene30629.t1 | MTR_3g434960 | 54.545 | 88 | 38 | 1 | 1 | 88 | 20 | 105 | 1.05e-26 | 95.1 |
| MS.gene30629.t1 | MTR_1g099810 | 50.833 | 120 | 42 | 3 | 1 | 104 | 1 | 119 | 2.34e-26 | 94.7 |
| MS.gene30629.t1 | MTR_1g099810 | 50.833 | 120 | 42 | 3 | 1 | 104 | 20 | 138 | 2.74e-26 | 95.1 |
| MS.gene30629.t1 | MTR_1g099815 | 53.448 | 116 | 38 | 3 | 1 | 104 | 20 | 131 | 1.43e-25 | 93.2 |
| MS.gene30629.t1 | MTR_3g435000 | 51.724 | 87 | 40 | 1 | 1 | 87 | 24 | 108 | 2.75e-25 | 91.7 |
| MS.gene30629.t1 | MTR_6g049260 | 55.682 | 88 | 37 | 2 | 1 | 88 | 20 | 105 | 3.17e-25 | 91.7 |
| MS.gene30629.t1 | MTR_3g434920 | 53.750 | 80 | 35 | 1 | 9 | 88 | 2 | 79 | 3.61e-23 | 85.5 |
| MS.gene30629.t1 | MTR_8g098915 | 45.536 | 112 | 50 | 3 | 1 | 105 | 1 | 108 | 9.61e-23 | 85.1 |
| MS.gene30629.t1 | MTR_8g098915 | 45.536 | 112 | 50 | 3 | 1 | 105 | 20 | 127 | 1.25e-22 | 85.5 |
| MS.gene30629.t1 | MTR_6g051680 | 72.549 | 51 | 14 | 0 | 1 | 51 | 20 | 70 | 1.31e-22 | 84.7 |
| MS.gene30629.t1 | MTR_1g102240 | 50.000 | 116 | 42 | 4 | 1 | 104 | 20 | 131 | 1.46e-22 | 85.5 |
| MS.gene30629.t1 | MTR_1g099640 | 44.348 | 115 | 50 | 2 | 1 | 105 | 20 | 130 | 3.85e-22 | 84.3 |
| MS.gene30629.t1 | MTR_0795s0020 | 47.826 | 115 | 43 | 3 | 1 | 105 | 20 | 127 | 2.41e-19 | 77.0 |
| MS.gene30629.t1 | MTR_1g099810 | 45.192 | 104 | 40 | 3 | 17 | 104 | 1 | 103 | 7.15e-16 | 67.4 |
| MS.gene30629.t1 | MTR_8g028615 | 65.957 | 47 | 16 | 0 | 3 | 49 | 22 | 68 | 1.34e-15 | 68.2 |
| MS.gene30629.t1 | MTR_7g079110 | 59.184 | 49 | 20 | 0 | 1 | 49 | 20 | 68 | 5.05e-15 | 67.0 |
| MS.gene30629.t1 | MTR_1g099640 | 40.404 | 99 | 45 | 2 | 17 | 105 | 1 | 95 | 4.16e-14 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30629.t1 | AT1G01490 | 61.538 | 52 | 19 | 1 | 1 | 51 | 20 | 71 | 2.71e-14 | 65.1 |
| MS.gene30629.t1 | AT1G01490 | 61.538 | 52 | 19 | 1 | 1 | 51 | 20 | 71 | 2.71e-14 | 65.1 |
| MS.gene30629.t1 | AT1G01490 | 61.538 | 52 | 19 | 1 | 1 | 51 | 47 | 98 | 3.30e-14 | 65.5 |
| MS.gene30629.t1 | AT5G52740 | 56.863 | 51 | 20 | 1 | 1 | 51 | 20 | 68 | 6.00e-11 | 55.5 |
Find 0 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTGAAATAATTAAGTAT+TGG | - | 43378:300625-300644 | MS.gene30629:intron | 10.0% |
| !! | TTTGAACGAATAATATATTA+AGG | - | 43378:300759-300778 | MS.gene30629:intron | 15.0% |
| !!! | TTATTTTGCTAAAATTGTAT+TGG | - | 43378:300994-301013 | MS.gene30629:intron | 15.0% |
| !! | ACATAATATGTTTGTATACA+CGG | - | 43378:300889-300908 | MS.gene30629:intron | 20.0% |
| !! | CGGAAACATTTAATATATTA+AGG | - | 43378:300730-300749 | MS.gene30629:intron | 20.0% |
| !! | TAGTTAAACATACCATAAAA+GGG | - | 43378:300804-300823 | MS.gene30629:intron | 20.0% |
| !! | TATTGGTATATTATTTACAG+GGG | - | 43378:301011-301030 | MS.gene30629:intron | 20.0% |
| !! | TTGTTGCATATGATAATAAT+GGG | + | 43378:301235-301254 | None:intergenic | 20.0% |
| !!! | ATGATATTCAAATTCTTTTG+AGG | + | 43378:300514-300533 | None:intergenic | 20.0% |
| !!! | GTATTGGTATATTATTTACA+GGG | - | 43378:301010-301029 | MS.gene30629:intron | 20.0% |
| !!! | TCAAAAATAGTTTCCTTTTA+CGG | + | 43378:300838-300857 | None:intergenic | 20.0% |
| !!! | TGTATTGGTATATTATTTAC+AGG | - | 43378:301009-301028 | MS.gene30629:intron | 20.0% |
| ! | CAGGAATATTGAATATATTG+TGG | + | 43378:301258-301277 | None:intergenic | 25.0% |
| ! | CTAGTTAAACATACCATAAA+AGG | - | 43378:300803-300822 | MS.gene30629:intron | 25.0% |
| ! | GTTGTTGCATATGATAATAA+TGG | + | 43378:301236-301255 | None:intergenic | 25.0% |
| ! | TACATGTAGTAAGTAAACTA+AGG | - | 43378:301097-301116 | MS.gene30629:intron | 25.0% |
| ! | TAGTAAGTAAACTAAGGAAA+TGG | - | 43378:301103-301122 | MS.gene30629:intron | 25.0% |
| ! | TGTTGCATATGATAATAATG+GGG | + | 43378:301234-301253 | None:intergenic | 25.0% |
| !! | CTTTGTCTAGTTTCAATATT+TGG | - | 43378:300315-300334 | MS.gene30629:CDS | 25.0% |
| AAGGGAATTATTACCGTAAA+AGG | - | 43378:300822-300841 | MS.gene30629:intron | 30.0% | |
| AGGCTTCAAGTAATTGAATT+TGG | + | 43378:301209-301228 | None:intergenic | 30.0% | |
| AGTTTACTTACTACATGTAC+TGG | + | 43378:301096-301115 | None:intergenic | 30.0% | |
| ATATTCCTGTGTTTCAAGAA+TGG | - | 43378:301269-301288 | MS.gene30629:intron | 30.0% | |
| ATGCTGAAATAGTTTCTGTT+GGG | - | 43378:301130-301149 | MS.gene30629:intron | 30.0% | |
| GACAAGAAATTAACCATAAC+TGG | - | 43378:301060-301079 | MS.gene30629:intron | 30.0% | |
| ! | ACGGTAATAATTCCCTTTTA+TGG | + | 43378:300819-300838 | None:intergenic | 30.0% |
| ! | CATATGATAATAATGGGGAT+AGG | + | 43378:301229-301248 | None:intergenic | 30.0% |
| ! | TTCTTCTTTTCATCTTTTGC+AGG | + | 43378:301156-301175 | None:intergenic | 30.0% |
| !! | ATCGTTTTTCACTGTTTATG+AGG | - | 43378:300467-300486 | MS.gene30629:CDS | 30.0% |
| !!! | CAAATTCTTTTGAGGTTACT+AGG | + | 43378:300506-300525 | None:intergenic | 30.0% |
| ACTCAAGTGGTAAAAGATTG+CGG | - | 43378:300710-300729 | MS.gene30629:intron | 35.0% | |
| CAAAGTTGACATTACCAGAA+AGG | + | 43378:300300-300319 | None:intergenic | 35.0% | |
| CATGCTGAAATAGTTTCTGT+TGG | - | 43378:301129-301148 | MS.gene30629:intron | 35.0% | |
| TTGTTCCATTCTTGAAACAC+AGG | + | 43378:301277-301296 | None:intergenic | 35.0% | |
| ! | GTTTTTCACTGTTTATGAGG+TGG | - | 43378:300470-300489 | MS.gene30629:CDS | 35.0% |
| !! | ATTTGGTTCTTCTTCACATC+AGG | + | 43378:301192-301211 | None:intergenic | 35.0% |
| AGAATGGAACAAGATCCTGT+TGG | - | 43378:301285-301304 | MS.gene30629:intron | 40.0% | |
| CAGCATATGATACATCCAAC+AGG | + | 43378:301303-301322 | None:intergenic | 40.0% | |
| CGACATAAGTGTAACTCAAG+TGG | - | 43378:300697-300716 | MS.gene30629:intron | 40.0% | |
| TTAAGGTCTAATTGTGCCCT+CGG | - | 43378:300776-300795 | MS.gene30629:intron | 40.0% | |
| ATGTTTAACTAGTGCCCCGA+GGG | + | 43378:300795-300814 | None:intergenic | 45.0% | |
| TAAGGTCTAATTGTGCCCTC+GGG | - | 43378:300777-300796 | MS.gene30629:intron | 45.0% | |
| TATGTTTAACTAGTGCCCCG+AGG | + | 43378:300796-300815 | None:intergenic | 45.0% | |
| TGGATCTGTGTCTCCAGTTA+TGG | + | 43378:301076-301095 | None:intergenic | 45.0% | |
| !!! | TATTTATTTTAATTGAAAAA+TGG | + | 43378:300563-300582 | None:intergenic | 5.0% |
| AAGACTGTCTCAGGCCTTTC+TGG | - | 43378:300283-300302 | MS.gene30629:CDS | 50.0% | |
| AAGGTCTAATTGTGCCCTCG+GGG | - | 43378:300778-300797 | MS.gene30629:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43378 | gene | 300280 | 301323 | 300280 | ID=MS.gene30629 |
| 43378 | mRNA | 300280 | 301323 | 300280 | ID=MS.gene30629.t1;Parent=MS.gene30629 |
| 43378 | exon | 301299 | 301323 | 301299 | ID=MS.gene30629.t1.exon1;Parent=MS.gene30629.t1 |
| 43378 | CDS | 301299 | 301323 | 301299 | ID=cds.MS.gene30629.t1;Parent=MS.gene30629.t1 |
| 43378 | exon | 300280 | 300572 | 300280 | ID=MS.gene30629.t1.exon2;Parent=MS.gene30629.t1 |
| 43378 | CDS | 300280 | 300572 | 300280 | ID=cds.MS.gene30629.t1;Parent=MS.gene30629.t1 |
| Gene Sequence |
| Protein sequence |
