Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30633.t1 | AFK43139.1 | 96.7 | 122 | 4 | 0 | 1 | 122 | 3 | 124 | 2.20E-59 | 238 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30633.t1 | O03982 | 64.9 | 74 | 25 | 1 | 1 | 73 | 3 | 76 | 4.3e-19 | 95.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30633.t1 | I3SS97 | 96.7 | 122 | 4 | 0 | 1 | 122 | 3 | 124 | 1.6e-59 | 238.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058526 | MS.gene30633 | 0.895403 | 9.91E-76 | -1.69E-46 |
| MS.gene058527 | MS.gene30633 | 0.889883 | 1.63E-73 | -1.69E-46 |
| MS.gene058528 | MS.gene30633 | 0.895921 | 6.05E-76 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30633.t1 | MTR_6g049280 | 95.082 | 122 | 6 | 0 | 1 | 122 | 33 | 154 | 1.33e-74 | 218 |
| MS.gene30633.t1 | MTR_6g049280 | 95.082 | 122 | 6 | 0 | 1 | 122 | 3 | 124 | 8.06e-74 | 215 |
| MS.gene30633.t1 | MTR_6g049320 | 95.082 | 122 | 6 | 0 | 1 | 122 | 33 | 154 | 9.30e-74 | 216 |
| MS.gene30633.t1 | MTR_6g049320 | 95.082 | 122 | 6 | 0 | 1 | 122 | 3 | 124 | 8.16e-73 | 213 |
| MS.gene30633.t1 | MTR_6g051690 | 60.630 | 127 | 43 | 3 | 1 | 122 | 3 | 127 | 6.65e-38 | 124 |
| MS.gene30633.t1 | MTR_7g451200 | 56.604 | 106 | 44 | 1 | 1 | 106 | 3 | 106 | 4.70e-37 | 122 |
| MS.gene30633.t1 | MTR_1g099800 | 56.391 | 133 | 46 | 2 | 1 | 121 | 3 | 135 | 2.56e-36 | 121 |
| MS.gene30633.t1 | MTR_1173s0010 | 56.190 | 105 | 44 | 1 | 1 | 105 | 3 | 105 | 6.48e-36 | 119 |
| MS.gene30633.t1 | MTR_7g451210 | 56.250 | 112 | 44 | 2 | 1 | 109 | 3 | 112 | 2.26e-35 | 117 |
| MS.gene30633.t1 | MTR_3g434890 | 56.190 | 105 | 44 | 1 | 1 | 105 | 3 | 105 | 9.14e-35 | 116 |
| MS.gene30633.t1 | MTR_6g049260 | 59.048 | 105 | 41 | 2 | 1 | 105 | 3 | 105 | 1.09e-34 | 116 |
| MS.gene30633.t1 | MTR_1g099810 | 51.471 | 136 | 51 | 2 | 1 | 121 | 3 | 138 | 1.26e-34 | 117 |
| MS.gene30633.t1 | MTR_3g434960 | 55.238 | 105 | 45 | 1 | 1 | 105 | 3 | 105 | 3.18e-34 | 115 |
| MS.gene30633.t1 | MTR_6g051680 | 77.941 | 68 | 15 | 0 | 1 | 68 | 3 | 70 | 1.15e-33 | 113 |
| MS.gene30633.t1 | MTR_1g099815 | 55.639 | 133 | 43 | 3 | 1 | 121 | 3 | 131 | 3.24e-33 | 113 |
| MS.gene30633.t1 | MTR_8g098915 | 49.612 | 129 | 54 | 3 | 1 | 122 | 3 | 127 | 1.35e-32 | 111 |
| MS.gene30633.t1 | MTR_1g099640 | 50.000 | 132 | 52 | 3 | 1 | 122 | 3 | 130 | 1.57e-32 | 111 |
| MS.gene30633.t1 | MTR_3g435000 | 53.465 | 101 | 45 | 1 | 4 | 104 | 10 | 108 | 7.56e-32 | 108 |
| MS.gene30633.t1 | MTR_1g102240 | 52.632 | 133 | 47 | 4 | 1 | 121 | 3 | 131 | 1.07e-31 | 109 |
| MS.gene30633.t1 | MTR_0795s0020 | 52.273 | 132 | 46 | 3 | 1 | 122 | 3 | 127 | 1.24e-28 | 101 |
| MS.gene30633.t1 | MTR_1g099810 | 50.420 | 119 | 44 | 2 | 18 | 121 | 1 | 119 | 2.01e-26 | 95.5 |
| MS.gene30633.t1 | MTR_7g079110 | 62.121 | 66 | 25 | 0 | 1 | 66 | 3 | 68 | 2.39e-23 | 89.4 |
| MS.gene30633.t1 | MTR_8g098915 | 46.429 | 112 | 49 | 3 | 18 | 122 | 1 | 108 | 7.87e-23 | 85.9 |
| MS.gene30633.t1 | MTR_3g434920 | 53.750 | 80 | 35 | 1 | 26 | 105 | 2 | 79 | 2.19e-22 | 84.3 |
| MS.gene30633.t1 | MTR_1g099810 | 44.660 | 103 | 42 | 2 | 34 | 121 | 1 | 103 | 6.28e-16 | 68.2 |
| MS.gene30633.t1 | MTR_1g099640 | 44.444 | 99 | 41 | 3 | 34 | 122 | 1 | 95 | 2.38e-15 | 66.6 |
| MS.gene30633.t1 | MTR_8g028615 | 66.667 | 66 | 22 | 0 | 1 | 66 | 3 | 68 | 2.17e-14 | 65.5 |
| MS.gene30633.t1 | MTR_3g082250 | 43.210 | 81 | 42 | 4 | 1 | 77 | 5 | 85 | 1.25e-13 | 62.8 |
| MS.gene30633.t1 | MTR_5g020960 | 30.719 | 153 | 74 | 4 | 1 | 121 | 4 | 156 | 8.18e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30633.t1 | AT1G01490 | 66.667 | 69 | 22 | 1 | 1 | 68 | 3 | 71 | 1.55e-24 | 92.4 |
| MS.gene30633.t1 | AT1G01490 | 66.667 | 69 | 22 | 1 | 1 | 68 | 3 | 71 | 1.55e-24 | 92.4 |
| MS.gene30633.t1 | AT1G01490 | 66.667 | 69 | 22 | 1 | 1 | 68 | 30 | 98 | 2.05e-24 | 92.8 |
| MS.gene30633.t1 | AT5G52740 | 58.209 | 67 | 26 | 1 | 2 | 68 | 4 | 68 | 1.01e-18 | 75.9 |
| MS.gene30633.t1 | AT5G52760 | 43.269 | 104 | 49 | 4 | 3 | 103 | 7 | 103 | 3.43e-16 | 69.7 |
| MS.gene30633.t1 | AT5G52750 | 44.615 | 65 | 36 | 0 | 1 | 65 | 5 | 69 | 2.81e-12 | 59.7 |
Find 0 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTAGCAAAAAAATTAA+AGG | + | 43378:416910-416929 | None:intergenic | 10.0% |
| !!! | ACTTTTTTTTTTTACAAATT+GGG | + | 43378:416646-416665 | None:intergenic | 10.0% |
| !!! | TATTTTATATATTATTTGCA+GGG | - | 43378:416931-416950 | MS.gene30633:intron | 10.0% |
| !!! | TTTTTGAAATAATTAAGTAT+TGG | - | 43378:416667-416686 | MS.gene30633:intron | 10.0% |
| !!! | ATGATATTTAAATTCTTTTG+AGG | + | 43378:416556-416575 | None:intergenic | 15.0% |
| !!! | ATTTTATATATTATTTGCAG+GGG | - | 43378:416932-416951 | MS.gene30633:intron | 15.0% |
| !!! | CACTTTTTTTTTTTACAAAT+TGG | + | 43378:416647-416666 | None:intergenic | 15.0% |
| !!! | GTATTTTATATATTATTTGC+AGG | - | 43378:416930-416949 | MS.gene30633:intron | 15.0% |
| !! | GAAATTAAACTCATAATTGT+CGG | - | 43378:416719-416738 | MS.gene30633:intron | 20.0% |
| ! | CGGAAACATTTAATATATCA+AGG | - | 43378:416772-416791 | MS.gene30633:intron | 25.0% |
| ! | GAGGAATATTGAATATATTG+TGG | + | 43378:417179-417198 | None:intergenic | 25.0% |
| !! | ACTTGTCTAGTTTCAATATT+TGG | - | 43378:416359-416378 | MS.gene30633:CDS | 25.0% |
| !! | TCTAGTTTCAATATTTGGTT+TGG | - | 43378:416364-416383 | MS.gene30633:CDS | 25.0% |
| !!! | TAAATTCTTTTGAGGTTACT+AGG | + | 43378:416548-416567 | None:intergenic | 25.0% |
| ATATTCCTCTGTTTCAAGAA+TGG | - | 43378:417190-417209 | MS.gene30633:CDS | 30.0% | |
| ATGCTGAAATAGTTTCTGTT+GGG | - | 43378:417051-417070 | MS.gene30633:intron | 30.0% | |
| ATTTGATTCTTCTTCACATC+AGG | + | 43378:417113-417132 | None:intergenic | 30.0% | |
| CATGTAGTAATAATGAGGAT+AGG | + | 43378:417150-417169 | None:intergenic | 30.0% | |
| GACAAGAAATTAACCTTAAC+TGG | - | 43378:416981-417000 | MS.gene30633:intron | 30.0% | |
| TACATGTAGTAAGCAAACTA+AGG | - | 43378:417018-417037 | MS.gene30633:intron | 30.0% | |
| TAGTAAGCAAACTAAGGAAA+TGG | - | 43378:417024-417043 | MS.gene30633:intron | 30.0% | |
| TGTTGCATGTAGTAATAATG+AGG | + | 43378:417155-417174 | None:intergenic | 30.0% | |
| ! | TTCTTCTTTTCATCTTTTGC+AGG | + | 43378:417077-417096 | None:intergenic | 30.0% |
| !! | TTCGTTTTTCACTGTTTATG+AGG | - | 43378:416509-416528 | MS.gene30633:CDS | 30.0% |
| !! | TTCTTAGTGTGTTTGAGTTT+TGG | - | 43378:416831-416850 | MS.gene30633:intron | 30.0% |
| ACTCAAGTGGTAAAAGATTG+CGG | - | 43378:416752-416771 | MS.gene30633:intron | 35.0% | |
| CAACAGAAACTATTTCAGCA+TGG | + | 43378:417052-417071 | None:intergenic | 35.0% | |
| CATGCTGAAATAGTTTCTGT+TGG | - | 43378:417050-417069 | MS.gene30633:intron | 35.0% | |
| TTGTTCCATTCTTGAAACAG+AGG | + | 43378:417198-417217 | None:intergenic | 35.0% | |
| ! | ACAAGTTGACATTACCAGAA+AGG | + | 43378:416345-416364 | None:intergenic | 35.0% |
| ! | AGTCTTCATAGCTTTTTGCT+TGG | + | 43378:416314-416333 | None:intergenic | 35.0% |
| ! | AGTTTGCTTACTACATGTAC+TGG | + | 43378:417017-417036 | None:intergenic | 35.0% |
| ! | GTTTTTCACTGTTTATGAGG+TGG | - | 43378:416512-416531 | MS.gene30633:CDS | 35.0% |
| AAAGCTATGAAGACTGTCTC+AGG | - | 43378:416319-416338 | MS.gene30633:CDS | 40.0% | |
| AATATATCAAGGCGCGTGTT+TGG | - | 43378:416783-416802 | MS.gene30633:intron | 40.0% | |
| AGAATGGAACAAGATCCTGT+TGG | - | 43378:417206-417225 | MS.gene30633:CDS | 40.0% | |
| ATATATCAAGGCGCGTGTTT+GGG | - | 43378:416784-416803 | MS.gene30633:intron | 40.0% | |
| TAGCATATGACACATCCAAC+AGG | + | 43378:417224-417243 | None:intergenic | 40.0% | |
| CGGCATAAGTGTAACTCAAG+TGG | - | 43378:416739-416758 | MS.gene30633:intron | 45.0% | |
| TGGATCTGTGTCTCCAGTTA+AGG | + | 43378:416997-417016 | None:intergenic | 45.0% | |
| !!! | TATTTATTTTAATTGAAAAA+TGG | + | 43378:416605-416624 | None:intergenic | 5.0% |
| AAGACTGTCTCAGGCCTTTC+TGG | - | 43378:416328-416347 | MS.gene30633:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43378 | gene | 416274 | 417244 | 416274 | ID=MS.gene30633 |
| 43378 | mRNA | 416274 | 417244 | 416274 | ID=MS.gene30633.t1;Parent=MS.gene30633 |
| 43378 | exon | 417169 | 417244 | 417169 | ID=MS.gene30633.t1.exon1;Parent=MS.gene30633.t1 |
| 43378 | CDS | 417169 | 417244 | 417169 | ID=cds.MS.gene30633.t1;Parent=MS.gene30633.t1 |
| 43378 | exon | 416274 | 416566 | 416274 | ID=MS.gene30633.t1.exon2;Parent=MS.gene30633.t1 |
| 43378 | CDS | 416274 | 416566 | 416274 | ID=cds.MS.gene30633.t1;Parent=MS.gene30633.t1 |
| Gene Sequence |
| Protein sequence |
