Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30678.t1 | XP_004488690.1 | 47.3 | 224 | 112 | 4 | 1 | 221 | 1 | 221 | 1.20E-45 | 193.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30678.t1 | P08688 | 38.1 | 226 | 130 | 7 | 6 | 223 | 7 | 230 | 4.7e-35 | 149.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30678.t1 | A0A1S2XHS6 | 47.3 | 224 | 112 | 4 | 1 | 221 | 1 | 221 | 8.4e-46 | 193.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054742 | MS.gene30678 | 0.807479 | 4.95E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene30678 | 0.809176 | 2.15E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30678.t1 | MTR_0807s0020 | 43.750 | 224 | 116 | 4 | 1 | 220 | 1 | 218 | 2.79e-58 | 183 |
| MS.gene30678.t1 | MTR_6g073540 | 45.778 | 225 | 113 | 3 | 1 | 216 | 1 | 225 | 3.53e-57 | 181 |
| MS.gene30678.t1 | MTR_6g045077 | 45.495 | 222 | 110 | 5 | 5 | 220 | 4 | 220 | 2.70e-55 | 176 |
| MS.gene30678.t1 | MTR_0695s0030 | 42.273 | 220 | 122 | 4 | 6 | 220 | 65 | 284 | 4.14e-55 | 178 |
| MS.gene30678.t1 | MTR_1g066530 | 41.704 | 223 | 126 | 2 | 1 | 221 | 1 | 221 | 2.75e-54 | 173 |
| MS.gene30678.t1 | MTR_6g048620 | 45.291 | 223 | 115 | 5 | 5 | 220 | 4 | 226 | 8.22e-54 | 172 |
| MS.gene30678.t1 | MTR_6g048910 | 46.188 | 223 | 109 | 6 | 5 | 220 | 4 | 222 | 1.56e-53 | 171 |
| MS.gene30678.t1 | MTR_8g038070 | 41.518 | 224 | 126 | 4 | 6 | 224 | 219 | 442 | 1.93e-53 | 178 |
| MS.gene30678.t1 | MTR_8g038100 | 42.727 | 220 | 121 | 4 | 6 | 220 | 281 | 500 | 2.82e-53 | 179 |
| MS.gene30678.t1 | MTR_6g072470 | 47.938 | 194 | 93 | 2 | 6 | 191 | 6 | 199 | 1.01e-52 | 169 |
| MS.gene30678.t1 | MTR_6g072470 | 34.559 | 136 | 77 | 3 | 68 | 192 | 14 | 148 | 2.96e-18 | 80.1 |
| MS.gene30678.t1 | MTR_0216s0030 | 43.005 | 193 | 104 | 3 | 6 | 194 | 3 | 193 | 2.66e-49 | 160 |
| MS.gene30678.t1 | MTR_0057s0100 | 40.271 | 221 | 125 | 6 | 6 | 220 | 7 | 226 | 1.25e-48 | 159 |
| MS.gene30678.t1 | MTR_0057s0170 | 39.819 | 221 | 126 | 6 | 6 | 220 | 7 | 226 | 3.13e-47 | 155 |
| MS.gene30678.t1 | MTR_6g040290 | 38.117 | 223 | 130 | 5 | 6 | 220 | 7 | 229 | 3.36e-41 | 140 |
| MS.gene30678.t1 | MTR_7g103990 | 38.739 | 222 | 128 | 7 | 6 | 220 | 7 | 227 | 5.33e-39 | 134 |
| MS.gene30678.t1 | MTR_7g012000 | 37.143 | 210 | 102 | 4 | 6 | 211 | 7 | 190 | 3.14e-37 | 129 |
| MS.gene30678.t1 | MTR_6g049040 | 45.578 | 147 | 58 | 5 | 1 | 143 | 1 | 129 | 5.02e-33 | 116 |
| MS.gene30678.t1 | MTR_6g049040 | 33.333 | 117 | 67 | 2 | 70 | 185 | 16 | 122 | 9.82e-13 | 63.5 |
| MS.gene30678.t1 | MTR_6g048810 | 32.911 | 237 | 124 | 6 | 5 | 209 | 4 | 237 | 1.17e-31 | 117 |
| MS.gene30678.t1 | MTR_6g048810 | 38.356 | 146 | 76 | 4 | 82 | 220 | 162 | 300 | 5.87e-21 | 89.0 |
| MS.gene30678.t1 | MTR_6g048880 | 39.106 | 179 | 99 | 4 | 48 | 220 | 4 | 178 | 6.81e-31 | 112 |
| MS.gene30678.t1 | MTR_4g102470 | 37.714 | 175 | 82 | 6 | 6 | 174 | 7 | 160 | 5.43e-26 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTGGTTTTAAATTATTAT+AGG | - | 42993:39871-39890 | MS.gene30678:CDS | 15.0% |
| !!! | TGTGGTTTTAAATTATTATA+GGG | - | 42993:39872-39891 | MS.gene30678:CDS | 15.0% |
| !! | GAAAAAAAAGAAATTGTTAG+TGG | - | 42993:39894-39913 | MS.gene30678:CDS | 20.0% |
| ! | GCTAACACTAAATTTGAAAA+AGG | - | 42993:39951-39970 | MS.gene30678:CDS | 25.0% |
| ! | TTACTGCTCCTATTAAAATT+GGG | - | 42993:40075-40094 | MS.gene30678:CDS | 25.0% |
| ! | TTTACTGCTCCTATTAAAAT+TGG | - | 42993:40074-40093 | MS.gene30678:CDS | 25.0% |
| AACACAAGTGACTATATTGT+CGG | - | 42993:40380-40399 | MS.gene30678:CDS | 30.0% | |
| AATTTAGTGTTAGCAAGCAT+TGG | + | 42993:39945-39964 | None:intergenic | 30.0% | |
| ATCTATTCCATGTTCAAAGT+GGG | + | 42993:40126-40145 | None:intergenic | 30.0% | |
| TACTGCTCCTATTAAAATTG+GGG | - | 42993:40076-40095 | MS.gene30678:CDS | 30.0% | |
| ! | AAAACGATGAGCAATCTTTT+CGG | + | 42993:40240-40259 | None:intergenic | 30.0% |
| ! | AATAGGAGCAGTAAACATTT+TGG | + | 42993:40069-40088 | None:intergenic | 30.0% |
| ! | ATCATTTTCTTCGACATCAA+AGG | + | 42993:39988-40007 | None:intergenic | 30.0% |
| !! | AACATCTCCCCAATTTTAAT+AGG | + | 42993:40086-40105 | None:intergenic | 30.0% |
| !! | ACGTGGAGTGTATTTTATTT+TGG | + | 42993:40039-40058 | None:intergenic | 30.0% |
| !!! | CATCGTTTTAGTTGTTTGTA+TGG | - | 42993:40251-40270 | MS.gene30678:CDS | 30.0% |
| !!! | CGTCGATTTTACATTTTCAA+AGG | - | 42993:40323-40342 | MS.gene30678:CDS | 30.0% |
| CATCTATTCCATGTTCAAAG+TGG | + | 42993:40127-40146 | None:intergenic | 35.0% | |
| GACAATATAGTCACTTGTGT+TGG | + | 42993:40381-40400 | None:intergenic | 35.0% | |
| TTTCGTGAAAGATCAGTATG+TGG | - | 42993:39854-39873 | MS.gene30678:CDS | 35.0% | |
| !! | TAACACGAATCAGTGTTGAT+TGG | - | 42993:40411-40430 | MS.gene30678:CDS | 35.0% |
| AAAGAAATTGTTAGTGGCCC+TGG | - | 42993:39900-39919 | MS.gene30678:CDS | 40.0% | |
| ACAAGTGACTATATTGTCGG+AGG | - | 42993:40383-40402 | MS.gene30678:CDS | 40.0% | |
| TATGGTACAGTGTTTGAGAG+TGG | - | 42993:40269-40288 | MS.gene30678:CDS | 40.0% | |
| TCTACCATCTCATGAAGAAC+AGG | + | 42993:40437-40456 | None:intergenic | 40.0% | |
| TGAATAAGAGAGCTCTCCAA+CGG | + | 42993:40166-40185 | None:intergenic | 40.0% | |
| TGGAGAGCTCTCTTATTCAA+AGG | - | 42993:40167-40186 | MS.gene30678:CDS | 40.0% | |
| TGTGCATTAATCGACTACAC+TGG | - | 42993:40353-40372 | MS.gene30678:CDS | 40.0% | |
| !! | GATGCAGCTTTTAGAGAGTA+TGG | - | 42993:40296-40315 | MS.gene30678:CDS | 40.0% |
| !!! | AACATTTTGGCTTCTGAACG+TGG | + | 42993:40056-40075 | None:intergenic | 40.0% |
| AACCCATCAGTAATATCGCC+AGG | + | 42993:39921-39940 | None:intergenic | 45.0% | |
| ACCCATCAGTAATATCGCCA+GGG | + | 42993:39920-39939 | None:intergenic | 45.0% | |
| ATTGATGTCCGACAGATGAC+TGG | + | 42993:39827-39846 | None:intergenic | 45.0% | |
| CTGCTGTTAGTTATGACCGT+TGG | - | 42993:40147-40166 | MS.gene30678:CDS | 45.0% | |
| GAGATGTTTCCTTGTCTCGT+GGG | - | 42993:40098-40117 | MS.gene30678:CDS | 45.0% | |
| TTGGCCTGTTCTTCATGAGA+TGG | - | 42993:40430-40449 | MS.gene30678:CDS | 45.0% | |
| ! | CCACTTCTCAAAGTCTTGTG+AGG | - | 42993:40211-40230 | MS.gene30678:CDS | 45.0% |
| ! | CCTCACAAGACTTTGAGAAG+TGG | + | 42993:40214-40233 | None:intergenic | 45.0% |
| GCCCTGGCGATATTACTGAT+GGG | - | 42993:39916-39935 | MS.gene30678:CDS | 50.0% | |
| GGAGATGTTTCCTTGTCTCG+TGG | - | 42993:40097-40116 | MS.gene30678:CDS | 50.0% | |
| GTGGGAACCCACTTTGAACA+TGG | - | 42993:40116-40135 | MS.gene30678:CDS | 50.0% | |
| TCCGACAGATGACTGGAATG+CGG | + | 42993:39820-39839 | None:intergenic | 50.0% | |
| GCCGCATTCCAGTCATCTGT+CGG | - | 42993:39816-39835 | MS.gene30678:CDS | 55.0% | |
| GGCCCTGGCGATATTACTGA+TGG | - | 42993:39915-39934 | MS.gene30678:CDS | 55.0% | |
| ! | AAGTGGGTTCCCACGAGACA+AGG | + | 42993:40110-40129 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42993 | gene | 39795 | 40469 | 39795 | ID=MS.gene30678 |
| 42993 | mRNA | 39795 | 40469 | 39795 | ID=MS.gene30678.t1;Parent=MS.gene30678 |
| 42993 | exon | 39795 | 40469 | 39795 | ID=MS.gene30678.t1.exon1;Parent=MS.gene30678.t1 |
| 42993 | CDS | 39795 | 40469 | 39795 | ID=cds.MS.gene30678.t1;Parent=MS.gene30678.t1 |
| Gene Sequence |
| Protein sequence |
