Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30816.t1 | XP_013452604.2 | 92.5 | 347 | 26 | 0 | 1 | 347 | 1 | 347 | 6.00E-174 | 620.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30816.t1 | Q84JE8 | 60.0 | 375 | 108 | 6 | 2 | 345 | 322 | 685 | 9.2e-115 | 414.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30816.t1 | A0A072UB23 | 92.5 | 347 | 26 | 0 | 1 | 347 | 1 | 347 | 4.3e-174 | 620.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene30816 | 0.823038 | 1.72E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30816.t1 | MTR_6g466500 | 93.948 | 347 | 21 | 0 | 1 | 347 | 1 | 347 | 0.0 | 639 |
| MS.gene30816.t1 | MTR_6g466500 | 93.948 | 347 | 21 | 0 | 1 | 347 | 27 | 373 | 0.0 | 638 |
| MS.gene30816.t1 | MTR_6g052000 | 88.462 | 104 | 11 | 1 | 1 | 104 | 1 | 103 | 6.21e-59 | 186 |
| MS.gene30816.t1 | MTR_6g052005 | 95.556 | 45 | 2 | 0 | 143 | 187 | 50 | 94 | 5.06e-23 | 92.0 |
| MS.gene30816.t1 | MTR_6g464930 | 76.786 | 56 | 4 | 1 | 253 | 308 | 40 | 86 | 5.50e-23 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30816.t1 | AT3G14890 | 59.467 | 375 | 110 | 6 | 2 | 345 | 322 | 685 | 2.07e-145 | 427 |
| MS.gene30816.t1 | AT3G14890 | 55.294 | 85 | 36 | 2 | 2 | 85 | 159 | 242 | 2.76e-14 | 74.3 |
| MS.gene30816.t1 | AT3G14890 | 57.647 | 85 | 33 | 3 | 8 | 91 | 50 | 132 | 3.02e-14 | 74.3 |
| MS.gene30816.t1 | AT3G14890 | 59.467 | 375 | 110 | 6 | 2 | 345 | 312 | 675 | 2.83e-145 | 426 |
| MS.gene30816.t1 | AT3G14890 | 55.294 | 85 | 36 | 2 | 2 | 85 | 159 | 242 | 3.03e-14 | 74.3 |
| MS.gene30816.t1 | AT3G14890 | 57.647 | 85 | 33 | 3 | 8 | 91 | 50 | 132 | 3.34e-14 | 74.3 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTAACCAATCCCAATCTT+AGG | 0.178998 | 6.4:+38523035 | None:intergenic |
| GTTCTTCCAACGATCAATAT+TGG | 0.242158 | 6.4:+38516068 | None:intergenic |
| AGTTGCCACATCCCTGGTTT+TGG | 0.274463 | 6.4:+38514402 | None:intergenic |
| TATAAGCTTTGCAATTTATC+AGG | 0.281391 | 6.4:+38516590 | None:intergenic |
| ACCAGGGATGTGGCAACTTA+TGG | 0.316391 | 6.4:-38514397 | MS.gene30816:CDS |
| AGCTGCATCACCGACATAAA+AGG | 0.318945 | 6.4:+38514184 | None:intergenic |
| GTCTGGAACGCCTTCCATTT+AGG | 0.329596 | 6.4:+38518547 | None:intergenic |
| CTTACCCTTTGTTCCAGCTT+TGG | 0.342514 | 6.4:+38514467 | None:intergenic |
| GAGGCTTTGATGTTACTAAA+TGG | 0.345137 | 6.4:-38523000 | MS.gene30816:CDS |
| CTCCGGCATTACCATCGATA+TGG | 0.355582 | 6.4:-38514361 | MS.gene30816:CDS |
| CACGAAGTTATTTGCTGAAC+AGG | 0.365764 | 6.4:-38521904 | MS.gene30816:CDS |
| GGAAGTGAAGGTCCCAATTC+AGG | 0.370405 | 6.4:-38515992 | MS.gene30816:intron |
| CATGCGGCAAAGTACTCTTC+AGG | 0.376017 | 6.4:+38513480 | None:intergenic |
| ACAATCTAAAACCCTAAGAT+TGG | 0.376386 | 6.4:-38523047 | MS.gene30816:CDS |
| TTTCTTTGAATCCCTTTCTT+CGG | 0.389015 | 6.4:+38520696 | None:intergenic |
| ACGCCTTCCATTTAGGCAAA+AGG | 0.414840 | 6.4:+38518554 | None:intergenic |
| TGTAACCAATCCCAATCTTA+GGG | 0.434916 | 6.4:+38523036 | None:intergenic |
| ATAACTTCGTGAGAGCTTCC+TGG | 0.434935 | 6.4:+38521916 | None:intergenic |
| GCTGGAACAAAGGGTAAGTC+TGG | 0.439275 | 6.4:-38514462 | MS.gene30816:CDS |
| TTTCAATGGTTGCAGGCAAA+TGG | 0.446269 | 6.4:-38513519 | MS.gene30816:intron |
| CGCCTTCCATTTAGGCAAAA+GGG | 0.450173 | 6.4:+38518555 | None:intergenic |
| TTGCAATTTATCAGGAATTG+AGG | 0.450407 | 6.4:+38516598 | None:intergenic |
| AAGGGTAAGTCTGGCAAAGC+TGG | 0.451313 | 6.4:-38514453 | MS.gene30816:CDS |
| GAGGAGTGAGAGGAGGGTGC+AGG | 0.451892 | 6.4:+38522959 | None:intergenic |
| TCCACCTCTGATGTCAAAGC+TGG | 0.452974 | 6.4:-38518775 | MS.gene30816:CDS |
| TAATTTGCAGGATGATGGTT+TGG | 0.455564 | 6.4:-38518341 | MS.gene30816:intron |
| TCCATAAGTTGCCACATCCC+TGG | 0.455866 | 6.4:+38514396 | None:intergenic |
| CAGAGCAAGATGCCGAAGAA+AGG | 0.459869 | 6.4:-38520708 | MS.gene30816:CDS |
| TCAGAGTAGGTCCAGATGCT+TGG | 0.460873 | 6.4:-38516634 | MS.gene30816:intron |
| GTGTTTCCAGAGCGGTGACC+AGG | 0.464804 | 6.4:-38521934 | MS.gene30816:intron |
| CAATCTAAAACCCTAAGATT+GGG | 0.467743 | 6.4:-38523046 | MS.gene30816:CDS |
| TAAAACCCTAAGATTGGGAT+TGG | 0.470686 | 6.4:-38523041 | MS.gene30816:CDS |
| TACATGAGAGACCAAGCATC+TGG | 0.487557 | 6.4:+38516623 | None:intergenic |
| GTCAGCCAAAGCTGGAACAA+AGG | 0.492870 | 6.4:-38514472 | MS.gene30816:CDS |
| ATCCATATCGATGGTAATGC+CGG | 0.503903 | 6.4:+38514359 | None:intergenic |
| TTTGATAATTTGCAGGATGA+TGG | 0.506720 | 6.4:-38518346 | MS.gene30816:intron |
| ACGATCAATATTGGATTCGT+TGG | 0.507287 | 6.4:+38516077 | None:intergenic |
| TCAGCCAAAGCTGGAACAAA+GGG | 0.508080 | 6.4:-38514471 | MS.gene30816:CDS |
| ACAGGTAAGTCAGCCAAAGC+TGG | 0.508804 | 6.4:-38514480 | MS.gene30816:CDS |
| AGGAGTGAGAGGAGGGTGCA+GGG | 0.515843 | 6.4:+38522960 | None:intergenic |
| TGCAATTTATCAGGAATTGA+GGG | 0.516407 | 6.4:+38516599 | None:intergenic |
| ATGCGGCAAAGTACTCTTCA+GGG | 0.542352 | 6.4:+38513481 | None:intergenic |
| TCCTCAGTTGATAAAATCAC+TGG | 0.544261 | 6.4:-38522941 | MS.gene30816:CDS |
| GGAAACCAAAACCAGGGATG+TGG | 0.546676 | 6.4:-38514407 | MS.gene30816:CDS |
| ACGTCTAAATCAGTTTATTG+AGG | 0.548165 | 6.4:-38516013 | MS.gene30816:CDS |
| AGAGCAAGATGCCGAAGAAA+GGG | 0.550468 | 6.4:-38520707 | MS.gene30816:CDS |
| AGGGTGCAGGGAAGCAAGTG+AGG | 0.555812 | 6.4:+38522972 | None:intergenic |
| CCACATATCAATGGCGCCGT+CGG | 0.557926 | 6.4:-38523137 | None:intergenic |
| TTTATGTCGGTGATGCAGCT+GGG | 0.560240 | 6.4:-38514181 | MS.gene30816:CDS |
| ATGGAGAAACACTTCAACTC+CGG | 0.561719 | 6.4:-38514378 | MS.gene30816:CDS |
| TCAGTTTATTGAGGAAGTGA+AGG | 0.562837 | 6.4:-38516004 | MS.gene30816:CDS |
| TTTATCAACTGAGGAGTGAG+AGG | 0.563190 | 6.4:+38522949 | None:intergenic |
| GTCGGAGTCGTCCGTTGTAG+CGG | 0.566031 | 6.4:-38523119 | MS.gene30816:CDS |
| AGCATTTCTTACACGTCGAA+CGG | 0.578226 | 6.4:+38523080 | None:intergenic |
| ACGAATCCAATATTGATCGT+TGG | 0.581245 | 6.4:-38516074 | MS.gene30816:CDS |
| TGATGTCAAGAGCACATACA+AGG | 0.586119 | 6.4:-38518728 | MS.gene30816:intron |
| ATCAACTGAGGAGTGAGAGG+AGG | 0.588802 | 6.4:+38522952 | None:intergenic |
| GAAGTTATTTGCTGAACAGG+AGG | 0.595985 | 6.4:-38521901 | MS.gene30816:CDS |
| CCACCTCTGATGTCAAAGCT+GGG | 0.596638 | 6.4:-38518774 | MS.gene30816:CDS |
| AACTAACTGATCCATATCGA+TGG | 0.597552 | 6.4:+38514350 | None:intergenic |
| TTGCAAAGCTTATACAACAA+TGG | 0.601553 | 6.4:-38516581 | MS.gene30816:CDS |
| CCCAGCTTTGACATCAGAGG+TGG | 0.602812 | 6.4:+38518774 | None:intergenic |
| TCAACTGAGGAGTGAGAGGA+GGG | 0.604902 | 6.4:+38522953 | None:intergenic |
| GTTACAAGAGACAAGAGTAG+AGG | 0.608203 | 6.4:-38523019 | MS.gene30816:CDS |
| GCCCAAATTATATGCTCATG+CGG | 0.608509 | 6.4:+38513464 | None:intergenic |
| ACAGGAGGGCAACTCTGAAG+AGG | 0.608524 | 6.4:-38521886 | MS.gene30816:intron |
| AAGTTATTTGCTGAACAGGA+GGG | 0.608900 | 6.4:-38521900 | MS.gene30816:CDS |
| AATCCCAGCTTTGACATCAG+AGG | 0.611765 | 6.4:+38518771 | None:intergenic |
| ATACAACAATGGCTACAAGC+TGG | 0.623223 | 6.4:-38516570 | MS.gene30816:intron |
| GCTACAACGGACGACTCCGA+CGG | 0.635767 | 6.4:+38523121 | None:intergenic |
| CTTCGCGTACTCCGCTACAA+CGG | 0.636368 | 6.4:+38523108 | None:intergenic |
| GTGCAGGGAAGCAAGTGAGG+TGG | 0.660439 | 6.4:+38522975 | None:intergenic |
| TGTTGATCTTAAATTTGCAG+AGG | 0.661189 | 6.4:-38514138 | MS.gene30816:intron |
| GAACAGCAGACAGAAAGCCG+TGG | 0.685426 | 6.4:-38516049 | MS.gene30816:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 38513479 | 38523149 | 38513479 | ID=MS.gene30816 |
| chr6.4 | mRNA | 38513479 | 38523149 | 38513479 | ID=MS.gene30816.t1;Parent=MS.gene30816 |
| chr6.4 | exon | 38522925 | 38523149 | 38522925 | ID=MS.gene30816.t1.exon1;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38522925 | 38523149 | 38522925 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38521887 | 38521946 | 38521887 | ID=MS.gene30816.t1.exon2;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38521887 | 38521946 | 38521887 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38520690 | 38520761 | 38520690 | ID=MS.gene30816.t1.exon3;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38520690 | 38520761 | 38520690 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38518729 | 38518800 | 38518729 | ID=MS.gene30816.t1.exon4;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38518729 | 38518800 | 38518729 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38518528 | 38518584 | 38518528 | ID=MS.gene30816.t1.exon5;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38518528 | 38518584 | 38518528 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38518277 | 38518353 | 38518277 | ID=MS.gene30816.t1.exon6;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38518277 | 38518353 | 38518277 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38516571 | 38516652 | 38516571 | ID=MS.gene30816.t1.exon7;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38516571 | 38516652 | 38516571 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38515993 | 38516109 | 38515993 | ID=MS.gene30816.t1.exon8;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38515993 | 38516109 | 38515993 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38514357 | 38514520 | 38514357 | ID=MS.gene30816.t1.exon9;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38514357 | 38514520 | 38514357 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38514139 | 38514208 | 38514139 | ID=MS.gene30816.t1.exon10;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38514139 | 38514208 | 38514139 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| chr6.4 | exon | 38513479 | 38513526 | 38513479 | ID=MS.gene30816.t1.exon11;Parent=MS.gene30816.t1 |
| chr6.4 | CDS | 38513479 | 38513526 | 38513479 | ID=cds.MS.gene30816.t1;Parent=MS.gene30816.t1 |
| Gene Sequence |
| Protein sequence |
