AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31157

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31157.t1 MTR_2g096740 96.393 305 6 4 1 302 1 303 0.0 592
MS.gene31157.t1 MTR_2g096740 91.475 305 6 5 1 302 1 288 0.0 553
MS.gene31157.t1 MTR_4g063780 43.099 355 119 17 1 296 1 331 8.48e-62 199
MS.gene31157.t1 MTR_4g022370 40.549 328 108 17 1 261 1 308 1.56e-47 163
MS.gene31157.t1 MTR_7g059400 37.818 275 115 6 1 238 1 256 3.32e-43 151
MS.gene31157.t1 MTR_7g024670 52.500 160 45 5 1 146 1 143 8.29e-42 148
MS.gene31157.t1 MTR_8g068210 56.000 100 41 1 55 154 12 108 5.57e-33 124
MS.gene31157.t1 MTR_8g479350 62.069 87 29 1 78 164 68 150 2.19e-32 122
MS.gene31157.t1 MTR_3g077750 52.475 101 48 0 28 128 11 111 2.90e-31 119
MS.gene31157.t1 MTR_4g088580 68.919 74 18 1 79 147 56 129 3.40e-31 120
MS.gene31157.t1 MTR_8g015840 76.119 67 14 1 73 139 15 79 9.13e-31 115
MS.gene31157.t1 MTR_4g461080 76.923 65 14 1 67 130 17 81 1.23e-30 116
MS.gene31157.t1 MTR_2g093220 64.935 77 27 0 56 132 18 94 1.42e-30 117
MS.gene31157.t1 MTR_5g031440 82.143 56 10 0 73 128 38 93 2.28e-30 117
MS.gene31157.t1 MTR_3g091820 71.642 67 19 0 67 133 32 98 2.32e-30 116
MS.gene31157.t1 MTR_3g090430 70.149 67 20 0 68 134 41 107 7.33e-30 115
MS.gene31157.t1 MTR_8g079060 52.000 100 44 2 71 166 22 121 1.18e-29 112
MS.gene31157.t1 MTR_4g109980 86.538 52 7 0 77 128 39 90 1.45e-29 114
MS.gene31157.t1 MTR_2g059540 90.000 50 5 0 77 126 30 79 2.00e-28 108
MS.gene31157.t1 MTR_8g027295 72.581 62 17 0 75 136 21 82 3.56e-28 110
MS.gene31157.t1 MTR_2g014170 75.362 69 17 0 77 145 44 112 4.15e-28 110
MS.gene31157.t1 MTR_2g014060 84.314 51 8 0 79 129 26 76 2.00e-27 109
MS.gene31157.t1 MTR_1g077600 68.333 60 19 0 75 134 40 99 6.74e-27 106
MS.gene31157.t1 MTR_1g077600 68.333 60 19 0 75 134 40 99 8.10e-27 106
MS.gene31157.t1 MTR_4g089095 75.862 58 13 1 78 135 50 106 8.76e-27 107
MS.gene31157.t1 MTR_4g089095 75.862 58 13 1 78 135 16 72 1.00e-26 105
MS.gene31157.t1 MTR_2g013370 50.538 93 27 2 42 134 28 101 1.13e-26 105
MS.gene31157.t1 MTR_1g056810 77.358 53 12 0 80 132 14 66 1.29e-26 106
MS.gene31157.t1 MTR_5g041420 44.118 102 57 0 30 131 90 191 7.32e-26 104
MS.gene31157.t1 MTR_6g012450 52.273 88 41 1 44 131 103 189 7.61e-26 107
MS.gene31157.t1 MTR_2g016030 56.410 78 30 1 64 141 37 110 9.93e-26 100
MS.gene31157.t1 MTR_8g044220 51.163 86 42 0 56 141 96 181 2.08e-25 105
MS.gene31157.t1 MTR_6g027450 58.824 68 28 0 65 132 76 143 4.35e-24 100
MS.gene31157.t1 MTR_7g010950 61.538 65 25 0 67 131 124 188 6.31e-24 101
MS.gene31157.t1 MTR_7g086780 59.701 67 27 0 65 131 86 152 1.16e-23 100
MS.gene31157.t1 MTR_3g435480 57.534 73 30 1 59 131 118 189 1.23e-23 100
MS.gene31157.t1 MTR_5g041530 47.778 90 47 0 42 131 104 193 1.63e-23 99.4
MS.gene31157.t1 MTR_6g027460 56.716 67 29 0 65 131 83 149 2.56e-23 98.6
MS.gene31157.t1 MTR_4g082060 41.481 135 72 3 75 207 119 248 3.00e-23 99.4
MS.gene31157.t1 MTR_5g041380 47.126 87 45 1 45 131 95 180 1.68e-21 93.6
MS.gene31157.t1 MTR_5g041400 53.333 75 32 1 57 131 101 172 6.79e-21 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31157.t1 AT3G55370 39.498 319 157 14 1 290 1 312 1.05e-47 162
MS.gene31157.t1 AT3G55370 42.975 242 114 9 1 224 1 236 7.84e-46 158
MS.gene31157.t1 AT3G55370 37.742 310 158 13 9 290 55 357 4.97e-41 146
MS.gene31157.t1 AT2G37590 51.351 148 55 4 1 134 1 145 1.41e-40 144
MS.gene31157.t1 AT2G28810 48.408 157 41 4 1 129 1 145 1.00e-37 137
MS.gene31157.t1 AT5G65590 72.500 80 17 1 65 139 23 102 8.27e-35 128
MS.gene31157.t1 AT5G02460 56.303 119 35 2 39 157 73 174 1.24e-34 130
MS.gene31157.t1 AT2G28810 61.702 94 24 1 36 129 47 128 7.33e-34 126
MS.gene31157.t1 AT1G07640 73.611 72 18 1 64 135 8 78 2.18e-33 124
MS.gene31157.t1 AT5G60200 65.333 75 26 0 71 145 48 122 4.38e-33 122
MS.gene31157.t1 AT1G07640 73.611 72 18 1 64 135 64 134 4.99e-33 124
MS.gene31157.t1 AT1G07640 73.611 72 18 1 64 135 72 142 7.62e-33 124
MS.gene31157.t1 AT1G51700 47.934 121 63 0 53 173 8 128 1.88e-32 119
MS.gene31157.t1 AT5G60850 67.532 77 23 1 77 151 50 126 2.51e-32 122
MS.gene31157.t1 AT5G66940 56.322 87 38 0 64 150 18 104 1.04e-30 115
MS.gene31157.t1 AT3G45610 63.889 72 26 0 68 139 30 101 2.52e-30 115
MS.gene31157.t1 AT5G62940 78.333 60 13 0 69 128 64 123 1.10e-29 116
MS.gene31157.t1 AT2G28510 78.333 60 13 0 77 136 47 106 1.41e-29 114
MS.gene31157.t1 AT4G21050 57.647 85 33 1 64 148 13 94 1.51e-29 112
MS.gene31157.t1 AT3G21270 86.792 53 7 0 78 130 29 81 1.95e-29 111
MS.gene31157.t1 AT3G52440 63.158 76 28 0 74 149 21 96 2.72e-29 112
MS.gene31157.t1 AT3G52440 63.158 76 28 0 74 149 40 115 3.58e-29 112
MS.gene31157.t1 AT1G28310 77.586 58 13 0 78 135 27 84 4.91e-29 113
MS.gene31157.t1 AT4G38000 73.770 61 16 0 72 132 35 95 7.41e-29 111
MS.gene31157.t1 AT1G28310 77.586 58 13 0 78 135 51 108 7.78e-29 113
MS.gene31157.t1 AT1G21340 71.667 60 17 0 76 135 36 95 1.81e-28 110
MS.gene31157.t1 AT3G50410 74.576 59 15 0 78 136 30 88 8.22e-28 108
MS.gene31157.t1 AT4G24060 66.667 66 22 0 67 132 42 107 1.30e-27 110
MS.gene31157.t1 AT3G61850 41.667 144 76 4 22 165 20 155 5.62e-27 107
MS.gene31157.t1 AT3G61850 41.958 143 75 4 23 165 9 143 5.79e-27 107
MS.gene31157.t1 AT3G61850 41.958 143 75 4 23 165 9 143 5.79e-27 107
MS.gene31157.t1 AT3G61850 41.958 143 75 4 23 165 21 155 8.65e-27 107
MS.gene31157.t1 AT3G61850 53.398 103 38 4 63 165 51 143 8.74e-27 107
MS.gene31157.t1 AT1G47655 68.182 66 20 1 62 126 12 77 2.35e-26 103
MS.gene31157.t1 AT4G00940 77.358 53 12 0 80 132 70 122 2.73e-25 102
MS.gene31157.t1 AT4G00940 77.358 53 12 0 80 132 70 122 2.73e-25 102
MS.gene31157.t1 AT4G00940 77.358 53 12 0 80 132 70 122 2.73e-25 102
MS.gene31157.t1 AT1G64620 68.852 61 19 0 67 127 38 98 3.59e-25 103
MS.gene31157.t1 AT2G34140 70.175 57 17 0 75 131 55 111 7.55e-25 98.6
MS.gene31157.t1 AT1G29160 70.175 57 17 0 75 131 59 115 1.21e-24 98.2
MS.gene31157.t1 AT2G46590 78.431 51 11 0 75 125 65 115 2.15e-24 101
MS.gene31157.t1 AT2G46590 78.431 51 11 0 75 125 77 127 2.40e-24 101
MS.gene31157.t1 AT5G39660 58.108 74 30 1 58 131 119 191 5.72e-24 101
MS.gene31157.t1 AT5G39660 58.108 74 30 1 58 131 119 191 5.72e-24 101
MS.gene31157.t1 AT5G39660 58.108 74 30 1 58 131 119 191 5.72e-24 101
MS.gene31157.t1 AT3G47500 48.352 91 40 2 47 131 74 163 6.72e-24 101
MS.gene31157.t1 AT1G69570 63.492 63 23 0 75 137 129 191 2.07e-23 99.4
MS.gene31157.t1 AT1G26790 56.164 73 29 1 59 131 95 164 2.49e-23 99.0
MS.gene31157.t1 AT5G62430 47.126 87 43 1 45 131 24 107 3.41e-23 97.1
MS.gene31157.t1 AT4G21040 35.955 178 74 8 80 218 27 203 1.54e-22 94.0
MS.gene31157.t1 AT4G21080 61.905 63 20 1 80 142 27 85 1.09e-21 92.0
MS.gene31157.t1 AT4G21030 49.296 71 32 1 70 140 17 83 1.80e-15 73.9

Find 73 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGACATGTAGGCGTTAT+TGG 0.219753 2.4:-6632996 MS.gene31157:CDS
TTGAACCAACAACTACTTCT+AGG 0.230645 2.4:-6632585 MS.gene31157:CDS
TTCTCATTACTATTTGATAT+TGG 0.260955 2.4:+6632877 None:intergenic
CAGTTTCCTTTCATGAATAA+TGG 0.306796 2.4:-6632658 MS.gene31157:CDS
TCAACAATTTCACTTTGAAA+TGG 0.326972 2.4:+6632604 None:intergenic
TGAAGAGATGTCATGTAATT+AGG 0.330958 2.4:+6632784 None:intergenic
TTATTCTCTGACATATTCAT+TGG 0.339957 2.4:+6632493 None:intergenic
CAACAATTTCACTTTGAAAT+GGG 0.353913 2.4:+6632605 None:intergenic
ACAACCATTACAATAATTCA+TGG 0.381419 2.4:-6632471 MS.gene31157:CDS
GTTGGAAACATGTTTCAAAT+TGG 0.387187 2.4:-6632742 MS.gene31157:CDS
GGAAGCAACTGATGTTGATT+TGG 0.388221 2.4:+6633255 None:intergenic
AGCTTGATCCGGTATCGAAC+CGG 0.392442 2.4:+6633185 None:intergenic
TCATGGTCTTGCATCTTCTT+CGG 0.406074 2.4:-6632440 MS.gene31157:CDS
CAAGAGGAGTTGAAGTTGAA+TGG 0.407718 2.4:+6632833 None:intergenic
ATGGTGCAGGCTCAATTATC+AGG 0.408827 2.4:-6633209 MS.gene31157:CDS
GATGCCTGCATCGGTTAAAT+CGG 0.412501 2.4:-6632551 MS.gene31157:CDS
TGTAATTAGGTCTATTAGTT+GGG 0.420123 2.4:+6632797 None:intergenic
ATGTAATTAGGTCTATTAGT+TGG 0.434589 2.4:+6632796 None:intergenic
CTTATTTAGATCCTCTCAAT+TGG 0.441172 2.4:-6633483 MS.gene31157:CDS
AAGGAGGAGTTGTGGATCAG+TGG 0.442180 2.4:-6632690 MS.gene31157:CDS
CATTCAACTTCAACTCCTCT+TGG 0.446006 2.4:-6632832 MS.gene31157:CDS
TGAAGTTCAAACTGGTCATC+AGG 0.450714 2.4:-6633145 MS.gene31157:CDS
TATCAGGCCCGGTTCGATAC+CGG 0.455922 2.4:-6633193 MS.gene31157:CDS
CTACGACACCCTCCACCAAC+AGG 0.460856 2.4:+6632946 None:intergenic
AAAAGTGTCTTGGTTGAGAC+AGG 0.461945 2.4:+6633019 None:intergenic
TTGAGAGGATCTAAATAAGA+TGG 0.471759 2.4:+6633487 None:intergenic
CAGTCCATGAATTATTGTAA+TGG 0.478494 2.4:+6632467 None:intergenic
TCAAGTACTGGTGGAGGAGA+AGG 0.484627 2.4:-6632709 MS.gene31157:CDS
ACATGTTTCCAACACCGAAC+CGG 0.484940 2.4:+6632752 None:intergenic
GGTGATTATAGTTCAAGTAC+TGG 0.489772 2.4:-6632721 MS.gene31157:CDS
TGAACCAACAACTACTTCTA+GGG 0.497338 2.4:-6632584 MS.gene31157:CDS
GCCTGAGCTTGAGCTTGATC+CGG 0.498212 2.4:+6633174 None:intergenic
TGTTCCGATTTAACCGATGC+AGG 0.498710 2.4:+6632547 None:intergenic
GCAGCTCATGAAGTTCAAAC+TGG 0.502505 2.4:-6633153 MS.gene31157:CDS
CTTCAGAATCTGAACCGGTT+CGG 0.507338 2.4:-6632766 MS.gene31157:CDS
CATCTCTTCAGAATCTGAAC+CGG 0.507412 2.4:-6632771 MS.gene31157:CDS
AATCTGAACCGGTTCGGTGT+TGG 0.507539 2.4:-6632760 MS.gene31157:CDS
TACTACTTTGATGCCTGCAT+CGG 0.514344 2.4:-6632560 MS.gene31157:CDS
GTAACCCTAGAAGTAGTTGT+TGG 0.518165 2.4:+6632580 None:intergenic
TTAAGAAATGTTCCTGTTGG+TGG 0.525025 2.4:-6632958 MS.gene31157:CDS
ACCGGATCAAGCTCAAGCTC+AGG 0.531426 2.4:-6633175 MS.gene31157:CDS
CATGTCTTGCAAAAGTGTCT+TGG 0.535988 2.4:+6633009 None:intergenic
TCATCACAGCCACATGGTGC+AGG 0.545230 2.4:-6633222 MS.gene31157:CDS
TCAAAACCATTATTCATGAA+AGG 0.546534 2.4:+6632652 None:intergenic
CACTTCAAAGCTGTCTCTGA+AGG 0.551798 2.4:+6633093 None:intergenic
AGAAATGTTCCTGTTGGTGG+AGG 0.558097 2.4:-6632955 MS.gene31157:CDS
CTTACTTGCTGCCAATTGAG+AGG 0.568314 2.4:+6633472 None:intergenic
AAATCGGAACACAACGGAGG+AGG 0.573191 2.4:-6632535 MS.gene31157:CDS
GCATTAAGAAATGTTCCTGT+TGG 0.573758 2.4:-6632961 MS.gene31157:CDS
GCTTGATCCGGTATCGAACC+GGG 0.575393 2.4:+6633186 None:intergenic
GCAGGCTCAATTATCAGGCC+CGG 0.575596 2.4:-6633204 MS.gene31157:CDS
AAGGTGGTGGTAACTTAGCT+TGG 0.577015 2.4:+6633112 None:intergenic
AGTACTGGTGGAGGAGAAGG+AGG 0.578007 2.4:-6632706 MS.gene31157:CDS
AAAGCTGTCTCTGAAGGTGG+TGG 0.586444 2.4:+6633099 None:intergenic
TATAGTTCAAGTACTGGTGG+AGG 0.593548 2.4:-6632715 MS.gene31157:CDS
GATTATAGTTCAAGTACTGG+TGG 0.600312 2.4:-6632718 MS.gene31157:CDS
GTAGGCGTTATTGGACAAGA+GGG 0.605161 2.4:-6632987 MS.gene31157:CDS
TGTGGCTGTGATGAGGATAG+TGG 0.611946 2.4:+6633231 None:intergenic
TGGAGGAGAAGGAGGAGTTG+TGG 0.612099 2.4:-6632698 MS.gene31157:CDS
TGTAGGCGTTATTGGACAAG+AGG 0.616737 2.4:-6632988 MS.gene31157:CDS
TTCAAAGCTGTCTCTGAAGG+TGG 0.619079 2.4:+6633096 None:intergenic
GAAATGTTCCTGTTGGTGGA+GGG 0.644271 2.4:-6632954 MS.gene31157:CDS
CTATCCTCATCACAGCCACA+TGG 0.646501 2.4:-6633228 MS.gene31157:CDS
GGCTGTGATGAGGATAGTGG+TGG 0.646948 2.4:+6633234 None:intergenic
TGCACCATGTGGCTGTGATG+AGG 0.663808 2.4:+6633224 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 2.4:-6632986 MS.gene31157:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 2.4:-6632985 MS.gene31157:CDS
ATAATTGAGCCTGCACCATG+TGG 0.676504 2.4:+6633213 None:intergenic
TGGAATAGAAGAGAAAACCA+TGG 0.712223 2.4:+6633507 None:intergenic
GGGTGTAAGAATTCACCAAG+AGG 0.726422 2.4:+6632817 None:intergenic
GTGTTGGTTGATTCACAACG+AGG 0.729681 2.4:+6633069 None:intergenic
GTTAAATCGGAACACAACGG+AGG 0.733463 2.4:-6632538 MS.gene31157:CDS
TCGGTTAAATCGGAACACAA+CGG 0.745806 2.4:-6632541 MS.gene31157:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGATATTATTATTACAA+AGG + chr2.4:6632534-6632553 None:intergenic 15.0%
!! TATTATTATTACAAAGGAAT+AGG + chr2.4:6632528-6632547 None:intergenic 15.0%
!! TCATATATAGATATATCACA+AGG + chr2.4:6632625-6632644 None:intergenic 20.0%
!! TTCTCATTACTATTTGATAT+TGG + chr2.4:6633054-6633073 None:intergenic 20.0%
!!! TAAATAAATTCACTCTTTTG+TGG + chr2.4:6632503-6632522 None:intergenic 20.0%
! ACAACCATTACAATAATTCA+TGG - chr2.4:6633457-6633476 MS.gene31157:intron 25.0%
! ATGTAATTAGGTCTATTAGT+TGG + chr2.4:6633135-6633154 None:intergenic 25.0%
! TCAAAACCATTATTCATGAA+AGG + chr2.4:6633279-6633298 None:intergenic 25.0%
! TGTAATGAAAAAAAATCTGC+AGG + chr2.4:6632565-6632584 None:intergenic 25.0%
! TGTAATTAGGTCTATTAGTT+GGG + chr2.4:6633134-6633153 None:intergenic 25.0%
! TTATTCTCTGACATATTCAT+TGG + chr2.4:6633438-6633457 None:intergenic 25.0%
!! ATTTGATATTGGAGATTTTG+AGG + chr2.4:6633043-6633062 None:intergenic 25.0%
!! GTAGTAACAAAATTTTGTGT+TGG + chr2.4:6632878-6632897 None:intergenic 25.0%
!! TCAACAATTTCACTTTGAAA+TGG + chr2.4:6633327-6633346 None:intergenic 25.0%
!! TTTGATATTGGAGATTTTGA+GGG + chr2.4:6633042-6633061 None:intergenic 25.0%
!!! CAACAATTTCACTTTGAAAT+GGG + chr2.4:6633326-6633345 None:intergenic 25.0%
ATATCACAAGGTTACAAGAA+AGG + chr2.4:6632613-6632632 None:intergenic 30.0%
CAGTCCATGAATTATTGTAA+TGG + chr2.4:6633464-6633483 None:intergenic 30.0%
CAGTTTCCTTTCATGAATAA+TGG - chr2.4:6633270-6633289 MS.gene31157:intron 30.0%
CTTATTTAGATCCTCTCAAT+TGG - chr2.4:6632445-6632464 MS.gene31157:CDS 30.0%
GTTGGAAACATGTTTCAAAT+TGG - chr2.4:6633186-6633205 MS.gene31157:CDS 30.0%
TATCACAAGGTTACAAGAAA+GGG + chr2.4:6632612-6632631 None:intergenic 30.0%
TGAAGAGATGTCATGTAATT+AGG + chr2.4:6633147-6633166 None:intergenic 30.0%
TTGAGAGGATCTAAATAAGA+TGG + chr2.4:6632444-6632463 None:intergenic 30.0%
!! AATTCATGGACTGATTTTCA+TGG - chr2.4:6633471-6633490 MS.gene31157:intron 30.0%
GCATTAAGAAATGTTCCTGT+TGG - chr2.4:6632967-6632986 MS.gene31157:CDS 35.0%
GGTGATTATAGTTCAAGTAC+TGG - chr2.4:6633207-6633226 MS.gene31157:CDS 35.0%
TGAACCAACAACTACTTCTA+GGG - chr2.4:6633344-6633363 MS.gene31157:intron 35.0%
TTAAGAAATGTTCCTGTTGG+TGG - chr2.4:6632970-6632989 MS.gene31157:CDS 35.0%
TTGAACCAACAACTACTTCT+AGG - chr2.4:6633343-6633362 MS.gene31157:intron 35.0%
!! GATTATAGTTCAAGTACTGG+TGG - chr2.4:6633210-6633229 MS.gene31157:CDS 35.0%
!!! AGTAGAGGTGTTACTCTTTT+TGG + chr2.4:6633019-6633038 None:intergenic 35.0%
CAAGAGGAGTTGAAGTTGAA+TGG + chr2.4:6633098-6633117 None:intergenic 40.0%
CATCTCTTCAGAATCTGAAC+CGG - chr2.4:6633157-6633176 MS.gene31157:CDS 40.0%
CATTCAACTTCAACTCCTCT+TGG - chr2.4:6633096-6633115 MS.gene31157:CDS 40.0%
GTAACCCTAGAAGTAGTTGT+TGG + chr2.4:6633351-6633370 None:intergenic 40.0%
TACTACTTTGATGCCTGCAT+CGG - chr2.4:6633368-6633387 MS.gene31157:intron 40.0%
TCGGTTAAATCGGAACACAA+CGG - chr2.4:6633387-6633406 MS.gene31157:intron 40.0%
TGAAGTTCAAACTGGTCATC+AGG - chr2.4:6632783-6632802 MS.gene31157:CDS 40.0%
! GACACTTTTGCAAGACATGT+AGG - chr2.4:6632923-6632942 MS.gene31157:CDS 40.0%
! GGAAGCAACTGATGTTGATT+TGG + chr2.4:6632676-6632695 None:intergenic 40.0%
! TCATGGTCTTGCATCTTCTT+CGG - chr2.4:6633488-6633507 MS.gene31157:CDS 40.0%
!! AAAAGTGTCTTGGTTGAGAC+AGG + chr2.4:6632912-6632931 None:intergenic 40.0%
!! CATGTCTTGCAAAAGTGTCT+TGG + chr2.4:6632922-6632941 None:intergenic 40.0%
!! TATAGTTCAAGTACTGGTGG+AGG - chr2.4:6633213-6633232 MS.gene31157:CDS 40.0%
ACATGTTTCCAACACCGAAC+CGG + chr2.4:6633179-6633198 None:intergenic 45.0%
ATAATTGAGCCTGCACCATG+TGG + chr2.4:6632718-6632737 None:intergenic 45.0%
ATGGTGCAGGCTCAATTATC+AGG - chr2.4:6632719-6632738 MS.gene31157:CDS 45.0%
CACTTCAAAGCTGTCTCTGA+AGG + chr2.4:6632838-6632857 None:intergenic 45.0%
CTTCAGAATCTGAACCGGTT+CGG - chr2.4:6633162-6633181 MS.gene31157:CDS 45.0%
GCAAGACATGTAGGCGTTAT+TGG - chr2.4:6632932-6632951 MS.gene31157:CDS 45.0%
GCAGCTCATGAAGTTCAAAC+TGG - chr2.4:6632775-6632794 MS.gene31157:CDS 45.0%
GGGTGTAAGAATTCACCAAG+AGG + chr2.4:6633114-6633133 None:intergenic 45.0%
GTAGGCGTTATTGGACAAGA+GGG - chr2.4:6632941-6632960 MS.gene31157:CDS 45.0%
GTTAAATCGGAACACAACGG+AGG - chr2.4:6633390-6633409 MS.gene31157:intron 45.0%
TAGGCGTTATTGGACAAGAG+GGG - chr2.4:6632942-6632961 MS.gene31157:CDS 45.0%
TGTAGGCGTTATTGGACAAG+AGG - chr2.4:6632940-6632959 MS.gene31157:CDS 45.0%
TGTTCCGATTTAACCGATGC+AGG + chr2.4:6633384-6633403 None:intergenic 45.0%
! AAGGTGGTGGTAACTTAGCT+TGG + chr2.4:6632819-6632838 None:intergenic 45.0%
! AGAAATGTTCCTGTTGGTGG+AGG - chr2.4:6632973-6632992 MS.gene31157:CDS 45.0%
! CTTACTTGCTGCCAATTGAG+AGG + chr2.4:6632459-6632478 None:intergenic 45.0%
! GAAATGTTCCTGTTGGTGGA+GGG - chr2.4:6632974-6632993 MS.gene31157:CDS 45.0%
! TTCAAAGCTGTCTCTGAAGG+TGG + chr2.4:6632835-6632854 None:intergenic 45.0%
!! GATGCCTGCATCGGTTAAAT+CGG - chr2.4:6633377-6633396 MS.gene31157:intron 45.0%
!! GTGTTGGTTGATTCACAACG+AGG + chr2.4:6632862-6632881 None:intergenic 45.0%
!! TGGAGATTTTGAGGGAGTAG+AGG + chr2.4:6633034-6633053 None:intergenic 45.0%
AAATCGGAACACAACGGAGG+AGG - chr2.4:6633393-6633412 MS.gene31157:intron 50.0%
AAGGAGGAGTTGTGGATCAG+TGG - chr2.4:6633238-6633257 MS.gene31157:CDS 50.0%
AGGCGTTATTGGACAAGAGG+GGG - chr2.4:6632943-6632962 MS.gene31157:CDS 50.0%
CTATCCTCATCACAGCCACA+TGG - chr2.4:6632700-6632719 MS.gene31157:CDS 50.0%
TGTGGCTGTGATGAGGATAG+TGG + chr2.4:6632700-6632719 None:intergenic 50.0%
! AAAGCTGTCTCTGAAGGTGG+TGG + chr2.4:6632832-6632851 None:intergenic 50.0%
! AGCTTGATCCGGTATCGAAC+CGG + chr2.4:6632746-6632765 None:intergenic 50.0%
!! AATCTGAACCGGTTCGGTGT+TGG - chr2.4:6633168-6633187 MS.gene31157:CDS 50.0%
!! TCAAGTACTGGTGGAGGAGA+AGG - chr2.4:6633219-6633238 MS.gene31157:CDS 50.0%
ACCGGATCAAGCTCAAGCTC+AGG - chr2.4:6632753-6632772 MS.gene31157:CDS 55.0%
GCAGGCTCAATTATCAGGCC+CGG - chr2.4:6632724-6632743 MS.gene31157:CDS 55.0%
GCCTGAGCTTGAGCTTGATC+CGG + chr2.4:6632757-6632776 None:intergenic 55.0%
TCATCACAGCCACATGGTGC+AGG - chr2.4:6632706-6632725 MS.gene31157:CDS 55.0%
TGCACCATGTGGCTGTGATG+AGG + chr2.4:6632707-6632726 None:intergenic 55.0%
TGGAGGAGAAGGAGGAGTTG+TGG - chr2.4:6633230-6633249 MS.gene31157:CDS 55.0%
! GCTTGATCCGGTATCGAACC+GGG + chr2.4:6632745-6632764 None:intergenic 55.0%
! GGCTGTGATGAGGATAGTGG+TGG + chr2.4:6632697-6632716 None:intergenic 55.0%
! TATCAGGCCCGGTTCGATAC+CGG - chr2.4:6632735-6632754 MS.gene31157:CDS 55.0%
!! AGTACTGGTGGAGGAGAAGG+AGG - chr2.4:6633222-6633241 MS.gene31157:CDS 55.0%
CTACGACACCCTCCACCAAC+AGG + chr2.4:6632985-6633004 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6632423 6633527 6632423 ID=MS.gene31157
chr2.4 mRNA 6632423 6633527 6632423 ID=MS.gene31157.t1;Parent=MS.gene31157
chr2.4 exon 6633477 6633527 6633477 ID=MS.gene31157.t1.exon1;Parent=MS.gene31157.t1
chr2.4 CDS 6633477 6633527 6633477 ID=cds.MS.gene31157.t1;Parent=MS.gene31157.t1
chr2.4 exon 6632423 6633280 6632423 ID=MS.gene31157.t1.exon2;Parent=MS.gene31157.t1
chr2.4 CDS 6632423 6633280 6632423 ID=cds.MS.gene31157.t1;Parent=MS.gene31157.t1
Gene Sequence

>MS.gene31157

ATGGTTTTCTCTTCTATTCCATCTTATTTAGATCCTCTCAATTGGCAGCAAGTAAGTTTTTTCTTTTTCTTTTTTTTCCACAAAAGAGTGAATTTATTTATTCCTATTCCTTTGTAATAATAATATCTTCTTGAATATACCTGCAGATTTTTTTTCATTACAACTCAAGAATAATTTCATCTTTTTCCCTTTCTTGTAACCTTGTGATATATCTATATATGAATTTCATTATACATATGTTTTGCAGCAACCAAATCAACATCAGTTGCTTCCACCACTATCCTCATCACAGCCACATGGTGCAGGCTCAATTATCAGGCCCGGTTCGATACCGGATCAAGCTCAAGCTCAGGCAGCTCATGAAGTTCAAACTGGTCATCAGGTTCAAACTCACCAAGCTAAGTTACCACCACCTTCAGAGACAGCTTTGAAGTGTCCTCGTTGTGAATCAACCAACACAAAATTTTGTTACTACAACAACTATAACCTGTCTCAACCAAGACACTTTTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCATTAAGAAATGTTCCTGTTGGTGGAGGGTGTCGTAGAAGCAAAAAGACCAAAAAGAGTAACACCTCTACTCCCTCAAAATCTCCAATATCAAATAGTAATGAGAAAGATTATTCAACAAGTGCAATCCATTCAACTTCAACTCCTCTTGGTGAATTCTTACACCCAACTAATAGACCTAATTACATGACATCTCTTCAGAATCTGAACCGGTTCGGTGTTGGAAACATGTTTCAAATTGGTGATTATAGTTCAAGTACTGGTGGAGGAGAAGGAGGAGTTGTGGATCAGTGGAGATTTCAGCAGTTTCCTTTCATGAATAATGGTTTTGATTCAAGTACTTCAAATGTTTCTTTCCCATTTCAAAGTGAAATTGTTGAACCAACAACTACTTCTAGGGTTACTACTTTGATGCCTGCATCGGTTAAATCGGAACACAACGGAGGAGGTTTTAATCTGCTTAGATCTCCAATGAATATGTCAGAGAATAACAACCATTACAATAATTCATGGACTGATTTTCATGGTCTTGCATCTTCTTCGGCTAGTCGTCTGTTATAA
Protein sequence

>MS.gene31157.t1

MVFSSIPSYLDPLNWQQQPNQHQLLPPLSSSQPHGAGSIIRPGSIPDQAQAQAAHEVQTGHQVQTHQAKLPPPSETALKCPRCESTNTKFCYYNNYNLSQPRHFCKTCRRYWTRGGALRNVPVGGGCRRSKKTKKSNTSTPSKSPISNSNEKDYSTSAIHSTSTPLGEFLHPTNRPNYMTSLQNLNRFGVGNMFQIGDYSSSTGGGEGGVVDQWRFQQFPFMNNGFDSSTSNVSFPFQSEIVEPTTTSRVTTLMPASVKSEHNGGGFNLLRSPMNMSENNNHYNNSWTDFHGLASSSASRLL