Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31452.t1 | RHN75755.1 | 78.9 | 175 | 33 | 1 | 1 | 171 | 1 | 175 | 4.70E-64 | 254.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31452.t1 | A0A396JHB0 | 78.9 | 175 | 33 | 1 | 1 | 171 | 1 | 175 | 3.4e-64 | 254.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054636 | MS.gene31452 | 0.81291 | 3.34E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31452.t1 | MTR_2g090555 | 80.460 | 174 | 30 | 1 | 1 | 170 | 1 | 174 | 3.56e-92 | 271 |
| MS.gene31452.t1 | MTR_2g090520 | 58.621 | 174 | 69 | 2 | 3 | 176 | 21 | 191 | 1.37e-62 | 195 |
| MS.gene31452.t1 | MTR_2g090520 | 58.621 | 174 | 69 | 2 | 3 | 176 | 21 | 191 | 4.18e-61 | 194 |
| MS.gene31452.t1 | MTR_2g090520 | 58.621 | 174 | 69 | 2 | 3 | 176 | 21 | 191 | 9.63e-61 | 194 |
| MS.gene31452.t1 | MTR_0370s0010 | 56.140 | 171 | 54 | 3 | 1 | 171 | 1 | 150 | 1.17e-53 | 172 |
| MS.gene31452.t1 | MTR_0370s0010 | 56.140 | 171 | 54 | 3 | 1 | 171 | 1 | 150 | 9.43e-53 | 172 |
| MS.gene31452.t1 | MTR_2g090525 | 53.684 | 190 | 65 | 5 | 1 | 176 | 1 | 181 | 1.25e-52 | 171 |
| MS.gene31452.t1 | MTR_2g090525 | 53.684 | 190 | 65 | 5 | 1 | 176 | 1 | 181 | 2.12e-52 | 171 |
| MS.gene31452.t1 | MTR_2g090525 | 61.039 | 77 | 27 | 2 | 100 | 176 | 1 | 74 | 1.47e-18 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTTGGTGAATTGAATTTC+AGG | 0.210142 | 2.4:-10111241 | MS.gene31452:CDS |
| GATATTGATTCTGGATCTAT+TGG | 0.257693 | 2.4:+10111558 | None:intergenic |
| TTTCACACCATATCTGATTC+TGG | 0.264582 | 2.4:+10110374 | None:intergenic |
| GTAGAATGGATGAACAAATT+TGG | 0.265621 | 2.4:+10111596 | None:intergenic |
| AAGACTATTGATGACAATTC+AGG | 0.300491 | 2.4:-10111627 | MS.gene31452:CDS |
| AGAAATGATCACAAAGAAAT+TGG | 0.309060 | 2.4:-10111475 | MS.gene31452:intron |
| CCTTAAGCACTTCTAGTGAT+TGG | 0.326958 | 2.4:+10110977 | None:intergenic |
| AACTATAAAAGATCATATTA+AGG | 0.368319 | 2.4:-10110500 | MS.gene31452:intron |
| GAAGCAAATGGAGGATGCTT+GGG | 0.404754 | 2.4:-10111209 | MS.gene31452:CDS |
| CCAATCACTAGAAGTGCTTA+AGG | 0.405041 | 2.4:-10110977 | MS.gene31452:CDS |
| GATACTAAGAGGTTTATATA+TGG | 0.410534 | 2.4:-10111264 | MS.gene31452:CDS |
| CTATTCTCCATACGGTGTCT+TGG | 0.434462 | 2.4:+10111293 | None:intergenic |
| ATCTAGATTCTGTCTTGAAT+CGG | 0.435568 | 2.4:-10111337 | MS.gene31452:CDS |
| TTGAATTATTTGAAGCTACA+TGG | 0.443340 | 2.4:-10111078 | MS.gene31452:CDS |
| AATAGAGAAAAGATACTAAG+AGG | 0.464194 | 2.4:-10111275 | MS.gene31452:CDS |
| AGAAGCAAATGGAGGATGCT+TGG | 0.472328 | 2.4:-10111210 | MS.gene31452:CDS |
| TGTGGTAGTTTCTATGTGAA+TGG | 0.486409 | 2.4:-10110410 | MS.gene31452:intron |
| ACTAGAAGTGCTTAAGGAAA+CGG | 0.498440 | 2.4:-10110971 | MS.gene31452:intron |
| AGGAGTATGAAAGAAGCAAA+TGG | 0.508483 | 2.4:-10111221 | MS.gene31452:CDS |
| GAAAGCTTGACAAAGTAGAA+TGG | 0.522169 | 2.4:+10111582 | None:intergenic |
| TCTGAGACCAGAATCAGATA+TGG | 0.523416 | 2.4:-10110381 | MS.gene31452:CDS |
| AGACTATTGATGACAATTCA+GGG | 0.531257 | 2.4:-10111626 | MS.gene31452:CDS |
| TGAATTATTTGAAGCTACAT+GGG | 0.531515 | 2.4:-10111077 | MS.gene31452:CDS |
| ATATTATCCAAGACACCGTA+TGG | 0.543146 | 2.4:-10111300 | MS.gene31452:CDS |
| AAGCAAATGGAGGATGCTTG+GGG | 0.595993 | 2.4:-10111208 | MS.gene31452:CDS |
| AGAGGTTTATATATGGCACT+TGG | 0.598402 | 2.4:-10111257 | MS.gene31452:CDS |
| GTAGTTTCTATGTGAATGGA+AGG | 0.613515 | 2.4:-10110406 | MS.gene31452:intron |
| AGTATGAAAGAAGCAAATGG+AGG | 0.695966 | 2.4:-10111218 | MS.gene31452:CDS |
| TGGGAGCAAAAGTTTCGCTG+AGG | 0.715877 | 2.4:-10111058 | MS.gene31452:CDS |
| AGCAAATGGAGGATGCTTGG+GGG | 0.725402 | 2.4:-10111207 | MS.gene31452:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTAAGGTAATTTATAAT+TGG | - | chr2.4:10111535-10111554 | MS.gene31452:CDS | 10.0% |
| !!! | TAATATATTTTTTGTTGTTT+TGG | - | chr2.4:10110897-10110916 | MS.gene31452:intron | 10.0% |
| !! | AACTATAAAAGATCATATTA+AGG | - | chr2.4:10111521-10111540 | MS.gene31452:CDS | 15.0% |
| !! | AATCAATATCAAAAATCAAA+AGG | - | chr2.4:10110476-10110495 | MS.gene31452:intron | 15.0% |
| !! | ATAAACATCAATAACTAAAA+AGG | - | chr2.4:10111199-10111218 | MS.gene31452:CDS | 15.0% |
| !! | ATATAACAGTTATTATGTAA+CGG | - | chr2.4:10111327-10111346 | MS.gene31452:CDS | 15.0% |
| !! | ATATTTAAATTTATCATGGA+TGG | + | chr2.4:10110603-10110622 | None:intergenic | 15.0% |
| !! | ATCAATATCAAAAATCAAAA+GGG | - | chr2.4:10110477-10110496 | MS.gene31452:intron | 15.0% |
| !! | ATCCATGATAAATTTAAATA+TGG | - | chr2.4:10110602-10110621 | MS.gene31452:intron | 15.0% |
| !! | ATGATAAATTTAAATATGGA+AGG | - | chr2.4:10110606-10110625 | MS.gene31452:intron | 15.0% |
| !! | TGATAAATTTAAATATGGAA+GGG | - | chr2.4:10110607-10110626 | MS.gene31452:intron | 15.0% |
| !! | TTCCATATTTAAATTTATCA+TGG | + | chr2.4:10110607-10110626 | None:intergenic | 15.0% |
| !!! | CTTTTTAGTTTTTTTTTTGA+GGG | + | chr2.4:10111234-10111253 | None:intergenic | 15.0% |
| !!! | GACATATATTTTATATTTTG+TGG | - | chr2.4:10111593-10111612 | MS.gene31452:CDS | 15.0% |
| !! | CCTCAAAAAAAAAACTAAAA+AGG | - | chr2.4:10111232-10111251 | MS.gene31452:CDS | 20.0% |
| !! | TGATGTTTATAGTACAAATT+AGG | + | chr2.4:10111189-10111208 | None:intergenic | 20.0% |
| !!! | ATCTTTTATAGTTTTCATCA+AGG | + | chr2.4:10111514-10111533 | None:intergenic | 20.0% |
| !!! | CCTTTTTAGTTTTTTTTTTG+AGG | + | chr2.4:10111235-10111254 | None:intergenic | 20.0% |
| !!! | TTGATTTTTGATATTGATTC+TGG | + | chr2.4:10110475-10110494 | None:intergenic | 20.0% |
| ! | AATAGAGAAAAGATACTAAG+AGG | - | chr2.4:10110746-10110765 | MS.gene31452:intron | 25.0% |
| ! | ACAATTCCAAATCAAACATA+TGG | - | chr2.4:10111437-10111456 | MS.gene31452:intron | 25.0% |
| ! | AGAAATGATCACAAAGAAAT+TGG | - | chr2.4:10110546-10110565 | MS.gene31452:intron | 25.0% |
| ! | GATACTAAGAGGTTTATATA+TGG | - | chr2.4:10110757-10110776 | MS.gene31452:intron | 25.0% |
| ! | TTATCGGAAAGTTAAAAACT+TGG | - | chr2.4:10111365-10111384 | MS.gene31452:intron | 25.0% |
| ! | TTTCATCAAGGATTTATAGT+TGG | + | chr2.4:10111502-10111521 | None:intergenic | 25.0% |
| !! | TGAATTATTTGAAGCTACAT+GGG | - | chr2.4:10110944-10110963 | MS.gene31452:intron | 25.0% |
| !! | TTGAATTATTTGAAGCTACA+TGG | - | chr2.4:10110943-10110962 | MS.gene31452:intron | 25.0% |
| !!! | TTCTTGTAATGATTTTGAGA+AGG | - | chr2.4:10111467-10111486 | MS.gene31452:intron | 25.0% |
| AAAACTTGGAGCAGATTAAA+CGG | - | chr2.4:10111379-10111398 | MS.gene31452:intron | 30.0% | |
| ATCTAGATTCTGTCTTGAAT+CGG | - | chr2.4:10110684-10110703 | MS.gene31452:intron | 30.0% | |
| GTAGAATGGATGAACAAATT+TGG | + | chr2.4:10110428-10110447 | None:intergenic | 30.0% | |
| GTCTTTCCATATGTTTGATT+TGG | + | chr2.4:10111446-10111465 | None:intergenic | 30.0% | |
| ! | AACAGTTATTATGTAACGGA+TGG | - | chr2.4:10111331-10111350 | MS.gene31452:CDS | 30.0% |
| ! | AAGACTATTGATGACAATTC+AGG | - | chr2.4:10110394-10110413 | MS.gene31452:CDS | 30.0% |
| ! | AGACTATTGATGACAATTCA+GGG | - | chr2.4:10110395-10110414 | MS.gene31452:CDS | 30.0% |
| ! | TCTTTTCTCTATTCTCCATA+CGG | + | chr2.4:10110739-10110758 | None:intergenic | 30.0% |
| !! | GATATTGATTCTGGATCTAT+TGG | + | chr2.4:10110466-10110485 | None:intergenic | 30.0% |
| AGAGGTTTATATATGGCACT+TGG | - | chr2.4:10110764-10110783 | MS.gene31452:intron | 35.0% | |
| AGTATGAAAGAAGCAAATGG+AGG | - | chr2.4:10110803-10110822 | MS.gene31452:intron | 35.0% | |
| ATATTATCCAAGACACCGTA+TGG | - | chr2.4:10110721-10110740 | MS.gene31452:intron | 35.0% | |
| GAAAGCTTGACAAAGTAGAA+TGG | + | chr2.4:10110442-10110461 | None:intergenic | 35.0% | |
| GATGGAGTATGCTCAATTAT+CGG | - | chr2.4:10111349-10111368 | MS.gene31452:CDS | 35.0% | |
| GTAGTTTCTATGTGAATGGA+AGG | - | chr2.4:10111615-10111634 | MS.gene31452:CDS | 35.0% | |
| GTGCATGTACTCAACATTAT+TGG | - | chr2.4:10111108-10111127 | MS.gene31452:intron | 35.0% | |
| TGTGGTAGTTTCTATGTGAA+TGG | - | chr2.4:10111611-10111630 | MS.gene31452:CDS | 35.0% | |
| ! | AGGAGTATGAAAGAAGCAAA+TGG | - | chr2.4:10110800-10110819 | MS.gene31452:intron | 35.0% |
| ! | CACTTGGTGAATTGAATTTC+AGG | - | chr2.4:10110780-10110799 | MS.gene31452:intron | 35.0% |
| !! | ACTAGAAGTGCTTAAGGAAA+CGG | - | chr2.4:10111050-10111069 | MS.gene31452:CDS | 35.0% |
| CCTTAAGCACTTCTAGTGAT+TGG | + | chr2.4:10111047-10111066 | None:intergenic | 40.0% | |
| ! | TCTGAGACCAGAATCAGATA+TGG | - | chr2.4:10111640-10111659 | MS.gene31452:CDS | 40.0% |
| !! | CCAATCACTAGAAGTGCTTA+AGG | - | chr2.4:10111044-10111063 | MS.gene31452:CDS | 40.0% |
| AAGCAAATGGAGGATGCTTG+GGG | - | chr2.4:10110813-10110832 | MS.gene31452:intron | 45.0% | |
| AGAAGCAAATGGAGGATGCT+TGG | - | chr2.4:10110811-10110830 | MS.gene31452:intron | 45.0% | |
| CTATTCTCCATACGGTGTCT+TGG | + | chr2.4:10110731-10110750 | None:intergenic | 45.0% | |
| GAAGCAAATGGAGGATGCTT+GGG | - | chr2.4:10110812-10110831 | MS.gene31452:intron | 45.0% | |
| AGCAAATGGAGGATGCTTGG+GGG | - | chr2.4:10110814-10110833 | MS.gene31452:intron | 50.0% | |
| TGGGAGCAAAAGTTTCGCTG+AGG | - | chr2.4:10110963-10110982 | MS.gene31452:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 10110376 | 10111667 | 10110376 | ID=MS.gene31452 |
| chr2.4 | mRNA | 10110376 | 10111667 | 10110376 | ID=MS.gene31452.t1;Parent=MS.gene31452 |
| chr2.4 | exon | 10111476 | 10111667 | 10111476 | ID=MS.gene31452.t1.exon1;Parent=MS.gene31452.t1 |
| chr2.4 | CDS | 10111476 | 10111667 | 10111476 | ID=cds.MS.gene31452.t1;Parent=MS.gene31452.t1 |
| chr2.4 | exon | 10111190 | 10111372 | 10111190 | ID=MS.gene31452.t1.exon2;Parent=MS.gene31452.t1 |
| chr2.4 | CDS | 10111190 | 10111372 | 10111190 | ID=cds.MS.gene31452.t1;Parent=MS.gene31452.t1 |
| chr2.4 | exon | 10110972 | 10111103 | 10110972 | ID=MS.gene31452.t1.exon3;Parent=MS.gene31452.t1 |
| chr2.4 | CDS | 10110972 | 10111103 | 10110972 | ID=cds.MS.gene31452.t1;Parent=MS.gene31452.t1 |
| chr2.4 | exon | 10110501 | 10110511 | 10110501 | ID=MS.gene31452.t1.exon4;Parent=MS.gene31452.t1 |
| chr2.4 | CDS | 10110501 | 10110511 | 10110501 | ID=cds.MS.gene31452.t1;Parent=MS.gene31452.t1 |
| chr2.4 | exon | 10110376 | 10110424 | 10110376 | ID=MS.gene31452.t1.exon5;Parent=MS.gene31452.t1 |
| chr2.4 | CDS | 10110376 | 10110424 | 10110376 | ID=cds.MS.gene31452.t1;Parent=MS.gene31452.t1 |
| Gene Sequence |
| Protein sequence |
