AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31769

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31769.t1 MTR_1g027950 70.679 324 90 4 1 322 1 321 9.98e-160 451
MS.gene31769.t1 MTR_1g027880 70.807 322 82 7 1 321 1 311 1.84e-151 427
MS.gene31769.t1 MTR_6g044970 63.804 326 78 9 1 321 1 291 1.92e-130 374
MS.gene31769.t1 MTR_1g027710 63.363 333 81 10 1 321 1 304 1.23e-127 367
MS.gene31769.t1 MTR_1g028250 50.825 303 107 8 18 314 6 272 9.38e-91 272
MS.gene31769.t1 MTR_1g027830 50.645 310 116 9 10 316 8 283 7.28e-89 280
MS.gene31769.t1 MTR_3g028640 51.203 291 89 9 36 316 14 261 1.65e-83 253
MS.gene31769.t1 MTR_1g027050 50.329 304 121 9 25 302 1 300 1.05e-82 265
MS.gene31769.t1 MTR_1g027140 49.664 298 104 12 36 304 25 305 1.20e-75 247
MS.gene31769.t1 MTR_1g027030 49.515 309 119 9 19 302 9 305 7.46e-74 241
MS.gene31769.t1 MTR_1g027030 49.515 309 119 9 19 302 9 305 2.09e-73 241
MS.gene31769.t1 MTR_1g027070 47.557 307 122 12 15 314 6 280 3.65e-72 237
MS.gene31769.t1 MTR_1g026950 38.636 308 139 8 20 309 1 276 6.58e-61 196
MS.gene31769.t1 MTR_1g027450 37.049 305 141 10 23 314 6 272 1.25e-55 182
MS.gene31769.t1 MTR_1g027260 38.790 281 125 7 34 297 14 264 2.04e-55 182
MS.gene31769.t1 MTR_1g026970 40.613 261 115 7 50 299 31 262 9.46e-54 178
MS.gene31769.t1 MTR_1g027520 38.007 271 119 8 19 275 3 238 2.69e-52 172
MS.gene31769.t1 MTR_1g027180 38.790 281 126 10 34 299 19 268 2.73e-51 173
MS.gene31769.t1 MTR_1g027380 36.786 280 135 6 50 321 30 275 1.26e-50 170
MS.gene31769.t1 MTR_1g027360 35.274 292 135 7 50 323 31 286 7.19e-49 166
MS.gene31769.t1 MTR_1g028090 37.102 283 124 9 47 310 29 276 1.28e-42 150
MS.gene31769.t1 MTR_1g027990 33.438 317 171 13 13 324 7 288 1.77e-40 150
MS.gene31769.t1 MTR_1g028150 36.059 269 121 9 47 301 29 260 3.40e-40 142
MS.gene31769.t1 MTR_1g028280 35.125 279 135 8 21 299 4 236 6.80e-40 149
MS.gene31769.t1 MTR_1g028280 35.125 279 135 8 21 299 4 236 7.88e-40 148
MS.gene31769.t1 MTR_1g027680 31.420 331 174 15 1 324 1 285 4.43e-39 146
MS.gene31769.t1 MTR_1g031510 33.913 230 123 8 53 279 35 238 7.19e-23 99.8
MS.gene31769.t1 MTR_1g031490 33.478 230 123 10 55 281 36 238 1.44e-20 90.1
MS.gene31769.t1 MTR_1g031535 29.021 286 153 12 20 302 4 242 5.98e-20 88.2
MS.gene31769.t1 MTR_1g031560 31.599 269 136 14 22 279 4 235 1.11e-19 90.1
MS.gene31769.t1 MTR_1g031450 29.795 292 151 13 14 302 2 242 1.57e-18 86.3
MS.gene31769.t1 MTR_1g031520 32.189 233 119 11 55 279 33 234 8.89e-17 81.3
MS.gene31769.t1 MTR_1g031280 30.739 257 144 12 39 287 22 252 2.10e-16 80.5
MS.gene31769.t1 MTR_1g031200 42.574 101 54 2 37 136 9 106 1.04e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 64 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAATATCAAATCGATTA+TGG 0.188286 1.1:+13603095 MS.gene31769:CDS
CAAATCGATTATGGGTATTA+TGG 0.274093 1.1:+13603104 MS.gene31769:CDS
ACTATTGTAAGAAGAGAAAT+TGG 0.275794 1.1:-13603057 None:intergenic
CATAATCGATTTGATATTGA+TGG 0.311110 1.1:-13603094 None:intergenic
GCAATCACCCTCTTCAATTC+CGG 0.338049 1.1:-13603003 None:intergenic
CAGCAACCACCATCACAATT+TGG 0.341915 1.1:-13602722 None:intergenic
AAAAGCATAATGATAGCTTT+TGG 0.351683 1.1:-13603222 None:intergenic
TCAAGCCGCAGTCTATATTA+TGG 0.355766 1.1:-13603511 None:intergenic
CTTTCATACGAGGAAATTCA+TGG 0.368428 1.1:+13603428 MS.gene31769:CDS
TATGAAGATGAAAACGATCT+TGG 0.379940 1.1:+13603269 MS.gene31769:CDS
AGAAATTGGTTGATGTTAAG+AGG 0.388044 1.1:-13603043 None:intergenic
ATCAATATCAAATCGATTAT+GGG 0.394020 1.1:+13603096 MS.gene31769:CDS
CAATCACCCTCTTCAATTCC+GGG 0.397957 1.1:-13603002 None:intergenic
GCTTGTTGCTATGACATCTT+CGG 0.427497 1.1:+13603361 MS.gene31769:CDS
AAGATGGTGGACAATATGTT+TGG 0.448816 1.1:-13602800 None:intergenic
TTCTGGGCTTCAATTCCAAA+TGG 0.457782 1.1:-13603596 None:intergenic
AAGCACTGCTGATCCATGAT+AGG 0.459048 1.1:-13603472 None:intergenic
GGGTCATTCTTAAGTCGAAA+TGG 0.459923 1.1:-13602852 None:intergenic
TTTGGAGTTCGAGTTGATGC+AGG 0.462929 1.1:-13603204 None:intergenic
TGATATTGATTCCGTAGAGC+AGG 0.467154 1.1:-13602685 None:intergenic
TTCATAACATTCAATATGTC+CGG 0.475216 1.1:-13603543 None:intergenic
CATGAATTTCCTCGTATGAA+AGG 0.482628 1.1:-13603427 None:intergenic
ATGAATTTCCTCGTATGAAA+GGG 0.482848 1.1:-13603426 None:intergenic
ATGAAGATGAAAACGATCTT+GGG 0.489524 1.1:+13603270 MS.gene31769:CDS
TCACAGGATAACTCATACCT+CGG 0.490964 1.1:-13602891 None:intergenic
GTTGATGTTAAGAGGTAATG+AGG 0.491694 1.1:-13603035 None:intergenic
TCGGATCACCACATGATGTT+GGG 0.492384 1.1:-13602872 None:intergenic
TCTACGGATTTAGCCTATCA+TGG 0.493184 1.1:+13603459 MS.gene31769:CDS
CTCGGATCACCACATGATGT+TGG 0.524972 1.1:-13602873 None:intergenic
GATATTGATTCCGTAGAGCA+GGG 0.543742 1.1:-13602684 None:intergenic
GATATCCTTCCCCTGCTCTA+CGG 0.545723 1.1:+13602674 MS.gene31769:CDS
CGATTATGGGTATTATGGCG+TGG 0.549654 1.1:+13603109 MS.gene31769:CDS
TGCTGCAGACTTCTACGTGA+GGG 0.554587 1.1:+13603244 MS.gene31769:CDS
GATTATGGGTATTATGGCGT+GGG 0.561296 1.1:+13603110 MS.gene31769:CDS
TCGTATGAAAGGGATCGATC+TGG 0.563858 1.1:-13603416 None:intergenic
ACAATTCAGGTAGTTGATCC+CGG 0.567342 1.1:+13602984 MS.gene31769:CDS
GAATGATGATCCTGAGTATG+TGG 0.574657 1.1:+13603172 MS.gene31769:CDS
TTGGAGTTCGAGTTGATGCA+GGG 0.579240 1.1:-13603203 None:intergenic
ATTCATGGAATGCTACTCTA+CGG 0.580096 1.1:+13603443 MS.gene31769:CDS
TCATAGCAACAAGCTTCACT+TGG 0.581621 1.1:-13603352 None:intergenic
TACAATAGTATCAACGAGGA+TGG 0.586698 1.1:+13603071 MS.gene31769:CDS
TTTGGGTAAATACTACGTGA+AGG 0.590105 1.1:+13602941 MS.gene31769:CDS
CTTCCTCCAAATTGTGATGG+TGG 0.595760 1.1:+13602716 MS.gene31769:CDS
TGTTAAGAGGTAATGAGGAA+TGG 0.598884 1.1:-13603030 None:intergenic
AGTTGATCCCGGAATTGAAG+AGG 0.607068 1.1:+13602995 MS.gene31769:CDS
GGAGCTGTATCCACATACTC+AGG 0.616776 1.1:-13603182 None:intergenic
TGCGGCTTGAATTACAGCAC+CGG 0.619496 1.1:+13603524 MS.gene31769:CDS
TTGCTGCAGACTTCTACGTG+AGG 0.627162 1.1:+13603243 MS.gene31769:CDS
TGTTCTCTGAAACTGACACT+AGG 0.632826 1.1:-13603389 None:intergenic
GTTGATCCCGGAATTGAAGA+GGG 0.640516 1.1:+13602996 MS.gene31769:CDS
AAGAATGACCCAACATCATG+TGG 0.641945 1.1:+13602864 MS.gene31769:CDS
CAACCACCATCACAATTTGG+AGG 0.645295 1.1:-13602719 None:intergenic
TGATTCCGTAGAGCAGGGGA+AGG 0.648059 1.1:-13602679 None:intergenic
ATGCTTCCTCCAAATTGTGA+TGG 0.648693 1.1:+13602713 MS.gene31769:CDS
ATTGAAGCCCAGAAAAGATG+TGG 0.649227 1.1:+13603605 MS.gene31769:CDS
CAACATCATGTGGTGATCCG+AGG 0.653095 1.1:+13602874 MS.gene31769:CDS
TGAAAACGATCTTGGGTACG+AGG 0.653942 1.1:+13603277 MS.gene31769:CDS
GGTACGAGGAGTCTTACTCT+CGG 0.657085 1.1:+13603291 MS.gene31769:CDS
AGTGCTTGCGAAGAATCTTG+CGG 0.666410 1.1:+13603488 MS.gene31769:CDS
ATATTGATTCCGTAGAGCAG+GGG 0.667424 1.1:-13602683 None:intergenic
AGATCGATCCCTTTCATACG+AGG 0.671208 1.1:+13603418 MS.gene31769:CDS
TGCGGCCATAATATAGACTG+CGG 0.685811 1.1:+13603506 MS.gene31769:CDS
TATTGTCCACCATCTTCCTG+CGG 0.721788 1.1:+13602807 MS.gene31769:CDS
TTCTTACAATAGTATCAACG+AGG 0.774326 1.1:+13603067 MS.gene31769:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTTTATTTTTAGAAATT+CGG + chr1.1:13603642-13603661 MS.gene31769:intron 10.0%
!! ATCAATATCAAATCGATTAT+GGG + chr1.1:13603096-13603115 MS.gene31769:CDS 20.0%
! ACTATTGTAAGAAGAGAAAT+TGG - chr1.1:13603060-13603079 None:intergenic 25.0%
! CAAAAATCATCATTTAGATC+TGG - chr1.1:13603569-13603588 None:intergenic 25.0%
! CATCAATATCAAATCGATTA+TGG + chr1.1:13603095-13603114 MS.gene31769:CDS 25.0%
! TTCATAACATTCAATATGTC+CGG - chr1.1:13603546-13603565 None:intergenic 25.0%
!!! AAAAGCATAATGATAGCTTT+TGG - chr1.1:13603225-13603244 None:intergenic 25.0%
!!! CATAATCGATTTGATATTGA+TGG - chr1.1:13603097-13603116 None:intergenic 25.0%
!!! GATTTTTGCTATTATCCATT+TGG + chr1.1:13603581-13603600 MS.gene31769:CDS 25.0%
ATGAAGATGAAAACGATCTT+GGG + chr1.1:13603270-13603289 MS.gene31769:CDS 30.0%
ATGAATTTCCTCGTATGAAA+GGG - chr1.1:13603429-13603448 None:intergenic 30.0%
CAAATCGATTATGGGTATTA+TGG + chr1.1:13603104-13603123 MS.gene31769:CDS 30.0%
CTACAAAAACTACACAATTC+AGG + chr1.1:13602971-13602990 MS.gene31769:CDS 30.0%
TATGAAGATGAAAACGATCT+TGG + chr1.1:13603269-13603288 MS.gene31769:CDS 30.0%
TTCTTACAATAGTATCAACG+AGG + chr1.1:13603067-13603086 MS.gene31769:CDS 30.0%
TTTACCCAAAAACAGAGTTA+AGG - chr1.1:13602931-13602950 None:intergenic 30.0%
! AGAAATTGGTTGATGTTAAG+AGG - chr1.1:13603046-13603065 None:intergenic 30.0%
! ATTTCTTACCACATCTTTTC+TGG - chr1.1:13603616-13603635 None:intergenic 30.0%
! TTTCTTACCACATCTTTTCT+GGG - chr1.1:13603615-13603634 None:intergenic 30.0%
!! ACAACCTTAACTCTGTTTTT+GGG + chr1.1:13602924-13602943 MS.gene31769:CDS 30.0%
ATTCATGGAATGCTACTCTA+CGG + chr1.1:13603443-13603462 MS.gene31769:CDS 35.0%
CATGAATTTCCTCGTATGAA+AGG - chr1.1:13603430-13603449 None:intergenic 35.0%
CTTTCATACGAGGAAATTCA+TGG + chr1.1:13603428-13603447 MS.gene31769:CDS 35.0%
GTTGATGTTAAGAGGTAATG+AGG - chr1.1:13603038-13603057 None:intergenic 35.0%
TACAATAGTATCAACGAGGA+TGG + chr1.1:13603071-13603090 MS.gene31769:CDS 35.0%
TGTTAAGAGGTAATGAGGAA+TGG - chr1.1:13603033-13603052 None:intergenic 35.0%
TTTGGGTAAATACTACGTGA+AGG + chr1.1:13602941-13602960 MS.gene31769:CDS 35.0%
! CACAACCTTAACTCTGTTTT+TGG + chr1.1:13602923-13602942 MS.gene31769:CDS 35.0%
!! AAGATGGTGGACAATATGTT+TGG - chr1.1:13602803-13602822 None:intergenic 35.0%
AAGAATGACCCAACATCATG+TGG + chr1.1:13602864-13602883 MS.gene31769:CDS 40.0%
ACAATTCAGGTAGTTGATCC+CGG + chr1.1:13602984-13603003 MS.gene31769:CDS 40.0%
ATGCTTCCTCCAAATTGTGA+TGG + chr1.1:13602713-13602732 MS.gene31769:CDS 40.0%
ATTGAAGCCCAGAAAAGATG+TGG + chr1.1:13603605-13603624 MS.gene31769:CDS 40.0%
GAATGATGATCCTGAGTATG+TGG + chr1.1:13603172-13603191 MS.gene31769:CDS 40.0%
GATTATGGGTATTATGGCGT+GGG + chr1.1:13603110-13603129 MS.gene31769:CDS 40.0%
GGAAAAACTACTTGTCAGCT+GGG + chr1.1:13603312-13603331 MS.gene31769:CDS 40.0%
GGGTCATTCTTAAGTCGAAA+TGG - chr1.1:13602855-13602874 None:intergenic 40.0%
TCAAGCCGCAGTCTATATTA+TGG - chr1.1:13603514-13603533 None:intergenic 40.0%
TCACAGGATAACTCATACCT+CGG - chr1.1:13602894-13602913 None:intergenic 40.0%
TCATAGCAACAAGCTTCACT+TGG - chr1.1:13603355-13603374 None:intergenic 40.0%
TCTACGGATTTAGCCTATCA+TGG + chr1.1:13603459-13603478 MS.gene31769:CDS 40.0%
TTCTGGGCTTCAATTCCAAA+TGG - chr1.1:13603599-13603618 None:intergenic 40.0%
! GCTTGTTGCTATGACATCTT+CGG + chr1.1:13603361-13603380 MS.gene31769:CDS 40.0%
!! ATATTGATTCCGTAGAGCAG+GGG - chr1.1:13602686-13602705 None:intergenic 40.0%
!! GATATTGATTCCGTAGAGCA+GGG - chr1.1:13602687-13602706 None:intergenic 40.0%
!! TGATATTGATTCCGTAGAGC+AGG - chr1.1:13602688-13602707 None:intergenic 40.0%
!! TGTTCTCTGAAACTGACACT+AGG - chr1.1:13603392-13603411 None:intergenic 40.0%
!!! GGTTGTGATGTTGTTTTCAC+AGG - chr1.1:13602910-13602929 None:intergenic 40.0%
AGATCGATCCCTTTCATACG+AGG + chr1.1:13603418-13603437 MS.gene31769:CDS 45.0%
AGTGCTTGCGAAGAATCTTG+CGG + chr1.1:13603488-13603507 MS.gene31769:CDS 45.0%
AGTTGATCCCGGAATTGAAG+AGG + chr1.1:13602995-13603014 MS.gene31769:CDS 45.0%
CAACCACCATCACAATTTGG+AGG - chr1.1:13602722-13602741 None:intergenic 45.0%
CAATCACCCTCTTCAATTCC+GGG - chr1.1:13603005-13603024 None:intergenic 45.0%
CAGCAACCACCATCACAATT+TGG - chr1.1:13602725-13602744 None:intergenic 45.0%
CGATTATGGGTATTATGGCG+TGG + chr1.1:13603109-13603128 MS.gene31769:CDS 45.0%
CGGAAAAACTACTTGTCAGC+TGG + chr1.1:13603311-13603330 MS.gene31769:CDS 45.0%
CTTCCTCCAAATTGTGATGG+TGG + chr1.1:13602716-13602735 MS.gene31769:CDS 45.0%
GCAATCACCCTCTTCAATTC+CGG - chr1.1:13603006-13603025 None:intergenic 45.0%
GTTGATCCCGGAATTGAAGA+GGG + chr1.1:13602996-13603015 MS.gene31769:CDS 45.0%
TATTGTCCACCATCTTCCTG+CGG + chr1.1:13602807-13602826 MS.gene31769:CDS 45.0%
TCGGATCACCACATGATGTT+GGG - chr1.1:13602875-13602894 None:intergenic 45.0%
TCGTATGAAAGGGATCGATC+TGG - chr1.1:13603419-13603438 None:intergenic 45.0%
TGAAAACGATCTTGGGTACG+AGG + chr1.1:13603277-13603296 MS.gene31769:CDS 45.0%
TGCGGCCATAATATAGACTG+CGG + chr1.1:13603506-13603525 MS.gene31769:CDS 45.0%
! AAGCACTGCTGATCCATGAT+AGG - chr1.1:13603475-13603494 None:intergenic 45.0%
! ATTTTTCCGCAGGAAGATGG+TGG - chr1.1:13602816-13602835 None:intergenic 45.0%
! CTGATTTTTCCGCAGGAAGA+TGG - chr1.1:13602819-13602838 None:intergenic 45.0%
!! TTGGAGTTCGAGTTGATGCA+GGG - chr1.1:13603206-13603225 None:intergenic 45.0%
!! TTTGGAGTTCGAGTTGATGC+AGG - chr1.1:13603207-13603226 None:intergenic 45.0%
!!! GATGTTGCTGATTTTTCCGC+AGG - chr1.1:13602826-13602845 None:intergenic 45.0%
CAACATCATGTGGTGATCCG+AGG + chr1.1:13602874-13602893 MS.gene31769:CDS 50.0%
CTCGGATCACCACATGATGT+TGG - chr1.1:13602876-13602895 None:intergenic 50.0%
GATATCCTTCCCCTGCTCTA+CGG + chr1.1:13602674-13602693 MS.gene31769:CDS 50.0%
GGAGCTGTATCCACATACTC+AGG - chr1.1:13603185-13603204 None:intergenic 50.0%
GGTACGAGGAGTCTTACTCT+CGG + chr1.1:13603291-13603310 MS.gene31769:CDS 50.0%
TGCGGCTTGAATTACAGCAC+CGG + chr1.1:13603524-13603543 MS.gene31769:CDS 50.0%
TGCTGCAGACTTCTACGTGA+GGG + chr1.1:13603244-13603263 MS.gene31769:CDS 50.0%
TTGCTGCAGACTTCTACGTG+AGG + chr1.1:13603243-13603262 MS.gene31769:CDS 50.0%
TGATTCCGTAGAGCAGGGGA+AGG - chr1.1:13602682-13602701 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 13602663 13603667 13602663 ID=MS.gene31769
chr1.1 mRNA 13602663 13603667 13602663 ID=MS.gene31769.t1;Parent=MS.gene31769
chr1.1 exon 13602663 13603626 13602663 ID=MS.gene31769.t1.exon1;Parent=MS.gene31769.t1
chr1.1 CDS 13602663 13603626 13602663 ID=cds.MS.gene31769.t1;Parent=MS.gene31769.t1
chr1.1 exon 13603657 13603667 13603657 ID=MS.gene31769.t1.exon2;Parent=MS.gene31769.t1
chr1.1 CDS 13603657 13603667 13603657 ID=cds.MS.gene31769.t1;Parent=MS.gene31769.t1
Gene Sequence

>MS.gene31769

ATGAGGAGAGGGATATCCTTCCCCTGCTCTACGGAATCAATATCACTATCATGCTTCCTCCAAATTGTGATGGTGGTTGCTGTGCTACTGTTGCATCATCATCATCAAACATGTTATGCAATTACAAATACTAATAACCAAACATATTGTCCACCATCTTCCTGCGGAAAAATCAGCAACATCAAACATCCATTTCGACTTAAGAATGACCCAACATCATGTGGTGATCCGAGGTATGAGTTATCCTGTGAAAACAACATCACAACCTTAACTCTGTTTTTGGGTAAATACTACGTGAAGGAAATAAACTACAAAAACTACACAATTCAGGTAGTTGATCCCGGAATTGAAGAGGGTGATTGCTCATCCATTCCTCATTACCTCTTAACATCAACCAATTTCTCTTCTTACAATAGTATCAACGAGGATGGCCATCAATATCAAATCGATTATGGGTATTATGGCGTGGGATACGCGTACATAATATACTTGAAGTGTAGCAAACAAGTGAATGATGATCCTGAGTATGTGGATACAGCTCCCTGCATCAACTCGAACTCCAAAAGCTATCATTATGCTTTTGCTGCAGACTTCTACGTGAGGGACTATGAAGATGAAAACGATCTTGGGTACGAGGAGTCTTACTCTCGGAAAAACTACTTGTCAGCTGGGAGATTAAAAGATTACTGCCAAGTGAAGCTTGTTGCTATGACATCTTCGGTTTTTCCTAGTGTCAGTTTCAGAGAACATGTACCAGATCGATCCCTTTCATACGAGGAAATTCATGGAATGCTACTCTACGGATTTAGCCTATCATGGATCAGCAGTGCTTGCGAAGAATCTTGCGGCCATAATATAGACTGCGGCTTGAATTACAGCACCGGACATATTGAATGTTATGAACCAGATCTAAATGATGATTTTTGCTATTATCCATTTGGAATTGAAGCCCAGAAAAGATGTGGTAAGAAATACTGTTTCTTTTTATTTTTAGAAATTCGGTAA
Protein sequence

>MS.gene31769.t1

MRRGISFPCSTESISLSCFLQIVMVVAVLLLHHHHQTCYAITNTNNQTYCPPSSCGKISNIKHPFRLKNDPTSCGDPRYELSCENNITTLTLFLGKYYVKEINYKNYTIQVVDPGIEEGDCSSIPHYLLTSTNFSSYNSINEDGHQYQIDYGYYGVGYAYIIYLKCSKQVNDDPEYVDTAPCINSNSKSYHYAFAADFYVRDYEDENDLGYEESYSRKNYLSAGRLKDYCQVKLVAMTSSVFPSVSFREHVPDRSLSYEEIHGMLLYGFSLSWISSACEESCGHNIDCGLNYSTGHIECYEPDLNDDFCYYPFGIEAQKRCEIR