Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31908.t1 | PNY04666.1 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 5.00E-76 | 293.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31908.t1 | Q8GYC1 | 91.7 | 133 | 11 | 0 | 4 | 136 | 64 | 196 | 1.3e-72 | 273.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31908.t1 | A0A2K3NNQ4 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 3.6e-76 | 293.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene31908.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057469 | MS.gene31908 | 0.802595 | 5.21E-49 | -1.69E-46 |
| MS.gene057929 | MS.gene31908 | 0.801005 | 1.10E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31908.t1 | MTR_1g112270 | 93.939 | 132 | 8 | 0 | 5 | 136 | 66 | 197 | 3.62e-93 | 280 |
| MS.gene31908.t1 | MTR_1g112270 | 93.233 | 133 | 9 | 0 | 4 | 136 | 54 | 186 | 6.23e-93 | 279 |
| MS.gene31908.t1 | MTR_1g094115 | 93.985 | 133 | 8 | 0 | 4 | 136 | 57 | 189 | 3.35e-92 | 276 |
| MS.gene31908.t1 | MTR_8g017210 | 87.218 | 133 | 17 | 0 | 4 | 136 | 72 | 204 | 1.20e-87 | 264 |
| MS.gene31908.t1 | MTR_2g099990 | 85.714 | 133 | 19 | 0 | 4 | 136 | 56 | 188 | 1.91e-86 | 259 |
| MS.gene31908.t1 | MTR_4g059870 | 83.582 | 134 | 21 | 1 | 4 | 136 | 48 | 181 | 2.35e-83 | 253 |
| MS.gene31908.t1 | MTR_1g016010 | 81.343 | 134 | 24 | 1 | 4 | 136 | 51 | 184 | 1.66e-82 | 249 |
| MS.gene31908.t1 | MTR_2g093960 | 84.328 | 134 | 20 | 1 | 4 | 136 | 42 | 175 | 6.86e-82 | 246 |
| MS.gene31908.t1 | MTR_2g090745 | 81.343 | 134 | 25 | 0 | 3 | 136 | 16 | 149 | 1.60e-81 | 246 |
| MS.gene31908.t1 | MTR_1g094115 | 93.103 | 116 | 8 | 0 | 21 | 136 | 57 | 172 | 1.68e-79 | 243 |
| MS.gene31908.t1 | MTR_2g099990 | 85.714 | 119 | 17 | 0 | 18 | 136 | 1 | 119 | 2.32e-77 | 234 |
| MS.gene31908.t1 | MTR_2g017720 | 73.276 | 116 | 26 | 1 | 3 | 118 | 322 | 432 | 3.82e-55 | 178 |
| MS.gene31908.t1 | MTR_3g102000 | 62.500 | 104 | 32 | 2 | 16 | 115 | 31 | 131 | 1.41e-38 | 130 |
| MS.gene31908.t1 | MTR_5g030560 | 80.435 | 46 | 9 | 0 | 3 | 48 | 15 | 60 | 1.09e-22 | 85.5 |
| MS.gene31908.t1 | MTR_1g093095 | 36.434 | 129 | 70 | 3 | 17 | 134 | 109 | 236 | 6.82e-19 | 80.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31908.t1 | AT2G02080 | 91.729 | 133 | 11 | 0 | 4 | 136 | 64 | 196 | 4.64e-94 | 281 |
| MS.gene31908.t1 | AT2G02080 | 91.729 | 133 | 11 | 0 | 4 | 136 | 64 | 196 | 4.64e-94 | 281 |
| MS.gene31908.t1 | AT2G02080 | 91.729 | 133 | 11 | 0 | 4 | 136 | 64 | 196 | 4.64e-94 | 281 |
| MS.gene31908.t1 | AT2G02080 | 91.729 | 133 | 11 | 0 | 4 | 136 | 64 | 196 | 4.64e-94 | 281 |
| MS.gene31908.t1 | AT1G14580 | 90.226 | 133 | 13 | 0 | 4 | 136 | 63 | 195 | 7.94e-91 | 271 |
| MS.gene31908.t1 | AT1G14580 | 90.226 | 133 | 13 | 0 | 4 | 136 | 63 | 195 | 7.94e-91 | 271 |
| MS.gene31908.t1 | AT1G14580 | 90.226 | 133 | 13 | 0 | 4 | 136 | 77 | 209 | 1.11e-90 | 271 |
| MS.gene31908.t1 | AT2G02070 | 91.729 | 133 | 11 | 0 | 4 | 136 | 62 | 194 | 1.43e-90 | 274 |
| MS.gene31908.t1 | AT2G02070 | 91.729 | 133 | 11 | 0 | 4 | 136 | 62 | 194 | 1.43e-90 | 274 |
| MS.gene31908.t1 | AT3G50700 | 86.466 | 133 | 18 | 0 | 4 | 136 | 44 | 176 | 5.31e-87 | 261 |
| MS.gene31908.t1 | AT2G02080 | 93.277 | 119 | 8 | 0 | 18 | 136 | 1 | 119 | 1.31e-85 | 256 |
| MS.gene31908.t1 | AT2G02080 | 93.277 | 119 | 8 | 0 | 18 | 136 | 1 | 119 | 1.31e-85 | 256 |
| MS.gene31908.t1 | AT5G66730 | 86.466 | 133 | 18 | 0 | 4 | 136 | 42 | 174 | 1.40e-85 | 258 |
| MS.gene31908.t1 | AT1G55110 | 86.567 | 134 | 17 | 1 | 4 | 136 | 73 | 206 | 5.17e-85 | 256 |
| MS.gene31908.t1 | AT1G55110 | 86.567 | 134 | 17 | 1 | 4 | 136 | 73 | 206 | 5.17e-85 | 256 |
| MS.gene31908.t1 | AT1G55110 | 86.567 | 134 | 17 | 1 | 4 | 136 | 73 | 206 | 5.17e-85 | 256 |
| MS.gene31908.t1 | AT3G45260 | 85.821 | 134 | 18 | 1 | 4 | 136 | 49 | 182 | 9.99e-84 | 252 |
| MS.gene31908.t1 | AT3G45260 | 85.821 | 134 | 18 | 1 | 4 | 136 | 49 | 182 | 9.99e-84 | 252 |
| MS.gene31908.t1 | AT3G13810 | 87.313 | 134 | 16 | 1 | 4 | 136 | 61 | 194 | 6.76e-83 | 251 |
| MS.gene31908.t1 | AT3G13810 | 87.313 | 134 | 16 | 1 | 4 | 136 | 80 | 213 | 7.04e-83 | 252 |
| MS.gene31908.t1 | AT3G13810 | 87.313 | 134 | 16 | 1 | 4 | 136 | 80 | 213 | 7.04e-83 | 252 |
| MS.gene31908.t1 | AT3G13810 | 87.313 | 134 | 16 | 1 | 4 | 136 | 75 | 208 | 7.22e-83 | 251 |
| MS.gene31908.t1 | AT5G03150 | 85.075 | 134 | 19 | 1 | 4 | 136 | 63 | 196 | 1.37e-82 | 251 |
| MS.gene31908.t1 | AT1G03840 | 81.955 | 133 | 24 | 0 | 4 | 136 | 51 | 183 | 1.54e-82 | 251 |
| MS.gene31908.t1 | AT3G13810 | 87.218 | 133 | 16 | 1 | 5 | 136 | 68 | 200 | 5.43e-82 | 249 |
| MS.gene31908.t1 | AT1G03840 | 81.203 | 133 | 25 | 0 | 4 | 136 | 49 | 181 | 5.95e-82 | 249 |
| MS.gene31908.t1 | AT3G13810 | 87.218 | 133 | 16 | 1 | 5 | 136 | 82 | 214 | 6.94e-82 | 249 |
| MS.gene31908.t1 | AT4G02670 | 83.582 | 134 | 21 | 1 | 4 | 136 | 65 | 198 | 1.85e-81 | 245 |
| MS.gene31908.t1 | AT4G02670 | 83.582 | 134 | 21 | 1 | 4 | 136 | 63 | 196 | 1.93e-81 | 245 |
| MS.gene31908.t1 | AT5G60470 | 82.090 | 134 | 23 | 1 | 4 | 136 | 54 | 187 | 3.11e-81 | 246 |
| MS.gene31908.t1 | AT5G60470 | 82.090 | 134 | 23 | 1 | 4 | 136 | 50 | 183 | 3.29e-81 | 246 |
| MS.gene31908.t1 | AT5G60470 | 81.955 | 133 | 23 | 1 | 5 | 136 | 46 | 178 | 7.45e-81 | 245 |
| MS.gene31908.t1 | AT5G44160 | 80.147 | 136 | 27 | 0 | 1 | 136 | 5 | 140 | 9.30e-81 | 244 |
| MS.gene31908.t1 | AT5G44160 | 81.955 | 133 | 24 | 0 | 4 | 136 | 47 | 179 | 1.64e-80 | 244 |
| MS.gene31908.t1 | AT1G25250 | 62.774 | 137 | 48 | 2 | 3 | 136 | 19 | 155 | 1.18e-63 | 198 |
| MS.gene31908.t1 | AT2G01940 | 63.704 | 135 | 47 | 2 | 4 | 136 | 54 | 188 | 3.82e-63 | 199 |
| MS.gene31908.t1 | AT1G25250 | 62.500 | 136 | 48 | 2 | 4 | 136 | 43 | 178 | 7.45e-63 | 197 |
| MS.gene31908.t1 | AT1G68130 | 62.963 | 135 | 48 | 2 | 4 | 136 | 51 | 185 | 7.98e-63 | 197 |
| MS.gene31908.t1 | AT2G01940 | 62.687 | 134 | 48 | 2 | 4 | 135 | 54 | 187 | 8.09e-61 | 193 |
| MS.gene31908.t1 | AT1G68130 | 63.636 | 99 | 34 | 2 | 40 | 136 | 1 | 99 | 1.26e-41 | 140 |
| MS.gene31908.t1 | AT2G01940 | 64.646 | 99 | 33 | 2 | 40 | 136 | 1 | 99 | 2.40e-41 | 140 |
| MS.gene31908.t1 | AT1G25250 | 62.000 | 100 | 35 | 2 | 40 | 136 | 1 | 100 | 8.27e-41 | 138 |
| MS.gene31908.t1 | AT1G34370 | 34.266 | 143 | 80 | 3 | 8 | 136 | 19 | 161 | 3.35e-20 | 84.0 |
| MS.gene31908.t1 | AT1G34370 | 34.266 | 143 | 80 | 3 | 8 | 136 | 229 | 371 | 2.00e-19 | 83.6 |
| MS.gene31908.t1 | AT1G34370 | 34.266 | 143 | 80 | 3 | 8 | 136 | 229 | 371 | 2.00e-19 | 83.6 |
| MS.gene31908.t1 | AT3G20880 | 33.835 | 133 | 79 | 3 | 10 | 134 | 243 | 374 | 1.99e-18 | 80.5 |
| MS.gene31908.t1 | AT5G22890 | 32.867 | 143 | 82 | 3 | 8 | 136 | 202 | 344 | 2.63e-18 | 79.7 |
| MS.gene31908.t1 | AT3G57670 | 35.338 | 133 | 77 | 4 | 10 | 134 | 204 | 335 | 3.70e-18 | 79.3 |
| MS.gene31908.t1 | AT1G51220 | 33.835 | 133 | 79 | 4 | 10 | 134 | 165 | 296 | 3.99e-18 | 79.0 |
| MS.gene31908.t1 | AT1G08290 | 34.694 | 147 | 80 | 6 | 3 | 134 | 154 | 299 | 1.66e-17 | 77.4 |
| MS.gene31908.t1 | AT1G34790 | 34.586 | 133 | 78 | 2 | 10 | 134 | 132 | 263 | 4.36e-17 | 75.9 |
| MS.gene31908.t1 | AT1G13290 | 35.938 | 128 | 71 | 4 | 17 | 134 | 95 | 221 | 6.43e-17 | 75.5 |
Find 25 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAAATAAACCTTTCTCTT+TGG | 0.255380 | 1.4:-19719545 | None:intergenic |
| GTTTGTTGCCATTAGGCTCT+TGG | 0.316327 | 1.4:-19719411 | None:intergenic |
| GTATGTGAAGTGTGCCAAAA+AGG | 0.353724 | 1.4:+19719440 | MS.gene31908:CDS |
| CAAATCTGTTTGTTGCCATT+AGG | 0.375978 | 1.4:-19719418 | None:intergenic |
| TTTGTTGCCATTAGGCTCTT+GGG | 0.385028 | 1.4:-19719410 | None:intergenic |
| TGGTGAACACATGTTGGTTC+TGG | 0.406411 | 1.4:-19719569 | None:intergenic |
| AGTGCTATCACTTCTGCATC+TGG | 0.408067 | 1.4:-19719383 | None:intergenic |
| AGTATGCTGTTCAATCTGAT+TGG | 0.446846 | 1.4:+19719690 | MS.gene31908:CDS |
| TTCTAAGACTTGTGGTACTA+AGG | 0.456129 | 1.4:+19719721 | MS.gene31908:CDS |
| GTGTTCACCATGACCCTTCA+AGG | 0.470912 | 1.4:+19719582 | MS.gene31908:CDS |
| ACTATCTCCCAAGAGCCTAA+TGG | 0.486645 | 1.4:+19719403 | MS.gene31908:CDS |
| GAAGAGGACACAATTTGCCT+TGG | 0.500777 | 1.4:+19719495 | MS.gene31908:CDS |
| TAACAAAGAGCCAAAGAGAA+AGG | 0.517289 | 1.4:+19719535 | MS.gene31908:CDS |
| ACGTATAAGTTGTTATTACC+TGG | 0.517908 | 1.4:-19719774 | None:intergenic |
| AGGGCACTTGGTGATCTCAC+TGG | 0.527339 | 1.4:+19719602 | MS.gene31908:CDS |
| CATGACCCTTCAAGGGCACT+TGG | 0.527782 | 1.4:+19719590 | MS.gene31908:CDS |
| AGATCACCAAGTGCCCTTGA+AGG | 0.560455 | 1.4:-19719596 | None:intergenic |
| ACTGTGGTACTCTCTTCTCC+AGG | 0.561759 | 1.4:+19719756 | MS.gene31908:CDS |
| CAAGTGCCCTTGAAGGGTCA+TGG | 0.580343 | 1.4:-19719589 | None:intergenic |
| GATCACCAAGTGCCCTTGAA+GGG | 0.585984 | 1.4:-19719595 | None:intergenic |
| AAAGCTCATTCTAAGACTTG+TGG | 0.613886 | 1.4:+19719713 | MS.gene31908:CDS |
| TGTTCACCATGACCCTTCAA+GGG | 0.614167 | 1.4:+19719583 | MS.gene31908:CDS |
| AATTTACAACTACATAGAAG+AGG | 0.622328 | 1.4:+19719479 | MS.gene31908:CDS |
| GGGTCATGGTGAACACATGT+TGG | 0.698022 | 1.4:-19719575 | None:intergenic |
| AAGGAGTATAGATGTGACTG+TGG | 0.709247 | 1.4:+19719740 | MS.gene31908:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTTACAACTACATAGAAG+AGG | + | chr1.4:19719479-19719498 | MS.gene31908:CDS | 25.0% |
| !!! | AAACACTATTTTAGAAAGCA+TGG | + | chr1.4:19719632-19719651 | MS.gene31908:CDS | 25.0% |
| CACAAATAAACCTTTCTCTT+TGG | - | chr1.4:19719548-19719567 | None:intergenic | 30.0% | |
| AGTATGCTGTTCAATCTGAT+TGG | + | chr1.4:19719690-19719709 | MS.gene31908:CDS | 35.0% | |
| TAACAAAGAGCCAAAGAGAA+AGG | + | chr1.4:19719535-19719554 | MS.gene31908:CDS | 35.0% | |
| TATGTGAAGTGTGCCAAAAA+GGG | + | chr1.4:19719441-19719460 | MS.gene31908:CDS | 35.0% | |
| ! | CAAATCTGTTTGTTGCCATT+AGG | - | chr1.4:19719421-19719440 | None:intergenic | 35.0% |
| ! | GAAAGCATGGTGAGAAAAAA+TGG | + | chr1.4:19719645-19719664 | MS.gene31908:CDS | 35.0% |
| ! | GTTGTAAATTTTGCTCCCTT+TGG | - | chr1.4:19719469-19719488 | None:intergenic | 35.0% |
| !! | AAAGCTCATTCTAAGACTTG+TGG | + | chr1.4:19719713-19719732 | MS.gene31908:CDS | 35.0% |
| !! | TTCTAAGACTTGTGGTACTA+AGG | + | chr1.4:19719721-19719740 | MS.gene31908:CDS | 35.0% |
| GTATGTGAAGTGTGCCAAAA+AGG | + | chr1.4:19719440-19719459 | MS.gene31908:CDS | 40.0% | |
| TTTGTTGCCATTAGGCTCTT+GGG | - | chr1.4:19719413-19719432 | None:intergenic | 40.0% | |
| ! | AAGGAGTATAGATGTGACTG+TGG | + | chr1.4:19719740-19719759 | MS.gene31908:CDS | 40.0% |
| !! | CTCTTTTGCTTTAGCTTCCA+AGG | - | chr1.4:19719515-19719534 | None:intergenic | 40.0% |
| ACTATCTCCCAAGAGCCTAA+TGG | + | chr1.4:19719403-19719422 | MS.gene31908:CDS | 45.0% | |
| AGTGCTATCACTTCTGCATC+TGG | - | chr1.4:19719386-19719405 | None:intergenic | 45.0% | |
| GAAGAGGACACAATTTGCCT+TGG | + | chr1.4:19719495-19719514 | MS.gene31908:CDS | 45.0% | |
| GTGCCAAAAAGGGTTCCAAA+GGG | + | chr1.4:19719451-19719470 | MS.gene31908:CDS | 45.0% | |
| TGTGCCAAAAAGGGTTCCAA+AGG | + | chr1.4:19719450-19719469 | MS.gene31908:CDS | 45.0% | |
| TGTTCACCATGACCCTTCAA+GGG | + | chr1.4:19719583-19719602 | MS.gene31908:CDS | 45.0% | |
| ! | GTTTGTTGCCATTAGGCTCT+TGG | - | chr1.4:19719414-19719433 | None:intergenic | 45.0% |
| !! | TGGTGAACACATGTTGGTTC+TGG | - | chr1.4:19719572-19719591 | None:intergenic | 45.0% |
| ACTGTGGTACTCTCTTCTCC+AGG | + | chr1.4:19719756-19719775 | MS.gene31908:CDS | 50.0% | |
| AGATCACCAAGTGCCCTTGA+AGG | - | chr1.4:19719599-19719618 | None:intergenic | 50.0% | |
| GATCACCAAGTGCCCTTGAA+GGG | - | chr1.4:19719598-19719617 | None:intergenic | 50.0% | |
| GGGTCATGGTGAACACATGT+TGG | - | chr1.4:19719578-19719597 | None:intergenic | 50.0% | |
| GTGTTCACCATGACCCTTCA+AGG | + | chr1.4:19719582-19719601 | MS.gene31908:CDS | 50.0% | |
| !!! | GCTCCCTTTGGAACCCTTTT+TGG | - | chr1.4:19719457-19719476 | None:intergenic | 50.0% |
| CAAGTGCCCTTGAAGGGTCA+TGG | - | chr1.4:19719592-19719611 | None:intergenic | 55.0% | |
| ! | AGGGCACTTGGTGATCTCAC+TGG | + | chr1.4:19719602-19719621 | MS.gene31908:CDS | 55.0% |
| !! | CATGACCCTTCAAGGGCACT+TGG | + | chr1.4:19719590-19719609 | MS.gene31908:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 19719371 | 19719781 | 19719371 | ID=MS.gene31908 |
| chr1.4 | mRNA | 19719371 | 19719781 | 19719371 | ID=MS.gene31908.t1;Parent=MS.gene31908 |
| chr1.4 | exon | 19719371 | 19719781 | 19719371 | ID=MS.gene31908.t1.exon1;Parent=MS.gene31908.t1 |
| chr1.4 | CDS | 19719371 | 19719781 | 19719371 | ID=cds.MS.gene31908.t1;Parent=MS.gene31908.t1 |
| Gene Sequence |
| Protein sequence |
