AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32221.t1 MTR_4g091510 98.240 625 11 0 1 625 1 625 0.0 1281
MS.gene32221.t1 MTR_4g091510 98.240 625 11 0 1 625 6 630 0.0 1281
MS.gene32221.t1 MTR_2g011640 75.917 627 146 2 1 625 52 675 0.0 982
MS.gene32221.t1 MTR_8g464860 59.160 595 224 6 42 625 36 622 0.0 707
MS.gene32221.t1 MTR_3g082750 29.781 319 195 7 224 537 212 506 7.71e-34 137
MS.gene32221.t1 MTR_3g094740 29.316 307 188 8 209 509 200 483 1.44e-28 121
MS.gene32221.t1 MTR_3g094740 29.316 307 188 8 209 509 200 483 1.63e-28 121
MS.gene32221.t1 MTR_8g091710 28.713 303 187 8 209 505 191 470 1.52e-26 115
MS.gene32221.t1 MTR_3g105760 24.938 405 261 9 211 601 123 498 3.47e-25 111
MS.gene32221.t1 MTR_6g038750 26.302 384 254 6 216 599 132 486 1.02e-23 106
MS.gene32221.t1 MTR_5g068050 26.346 353 225 8 216 554 128 459 2.01e-23 104
MS.gene32221.t1 MTR_3g070410 27.619 315 201 11 222 525 96 394 2.20e-22 102
MS.gene32221.t1 MTR_4g026450 24.289 387 255 8 222 599 161 518 5.29e-22 101
MS.gene32221.t1 MTR_1g046620 26.804 291 178 7 224 509 195 455 3.97e-21 98.6
MS.gene32221.t1 MTR_1g009860 27.899 276 172 6 224 495 142 394 4.52e-21 97.8
MS.gene32221.t1 MTR_1g009860 27.899 276 172 6 224 495 142 394 5.87e-21 97.8
MS.gene32221.t1 MTR_4g104070 29.260 311 172 11 224 515 79 360 3.21e-20 94.7
MS.gene32221.t1 MTR_1g008980 27.246 345 186 14 200 509 21 335 5.17e-20 95.1
MS.gene32221.t1 MTR_5g077080 26.460 291 182 9 227 511 98 362 4.50e-19 91.3
MS.gene32221.t1 MTR_0053s0030 27.835 291 180 7 224 509 151 416 1.19e-17 87.4
MS.gene32221.t1 MTR_1g095480 27.491 291 172 6 215 490 202 468 2.10e-16 83.6
MS.gene32221.t1 MTR_1g095480 28.846 260 158 5 215 459 202 449 4.15e-16 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32221.t1 AT1G11390 63.301 624 217 5 5 625 10 624 0.0 814
MS.gene32221.t1 AT1G61640 63.309 556 200 4 72 625 68 621 0.0 733
MS.gene32221.t1 AT1G61640 62.476 517 190 4 72 586 68 582 0.0 669
MS.gene32221.t1 AT1G61640 56.486 370 158 3 72 439 68 436 8.00e-145 429
MS.gene32221.t1 AT3G07700 28.793 323 199 7 224 543 201 495 1.12e-31 131
MS.gene32221.t1 AT3G07700 28.793 323 199 7 224 543 201 495 1.12e-31 131
MS.gene32221.t1 AT3G07700 28.793 323 199 7 224 543 201 495 1.12e-31 131
MS.gene32221.t1 AT3G07700 29.545 308 190 6 224 528 201 484 1.34e-31 131
MS.gene32221.t1 AT5G24970 26.136 440 276 11 184 601 148 560 1.56e-28 121
MS.gene32221.t1 AT5G24970 25.909 440 277 11 184 601 109 521 1.87e-28 121
MS.gene32221.t1 AT5G64940 28.990 307 189 8 209 509 215 498 1.32e-27 119
MS.gene32221.t1 AT5G64940 28.990 307 189 8 209 509 215 498 1.32e-27 119
MS.gene32221.t1 AT1G79600 30.537 298 179 9 216 509 150 423 1.52e-26 115
MS.gene32221.t1 AT1G65950 30.435 299 180 9 175 461 53 335 9.46e-26 111
MS.gene32221.t1 AT1G65950 30.435 299 180 9 175 461 53 335 2.37e-25 110
MS.gene32221.t1 AT5G24970 25.455 440 276 12 184 601 109 518 2.71e-25 111
MS.gene32221.t1 AT4G24810 29.299 314 170 8 224 516 33 315 7.57e-24 103
MS.gene32221.t1 AT5G50330 31.561 301 160 9 224 506 76 348 8.79e-24 105
MS.gene32221.t1 AT4G24810 29.393 313 169 8 224 515 33 314 1.61e-23 104
MS.gene32221.t1 AT4G24810 29.393 313 169 8 224 515 33 314 1.61e-23 104
MS.gene32221.t1 AT4G24810 29.393 313 169 8 224 515 76 357 2.17e-23 104
MS.gene32221.t1 AT4G24810 31.660 259 137 7 224 461 33 272 3.44e-23 101
MS.gene32221.t1 AT4G24810 29.063 320 168 9 224 515 33 321 2.28e-22 100
MS.gene32221.t1 AT1G71810 25.445 393 256 7 207 599 122 477 7.57e-22 100
MS.gene32221.t1 AT5G05200 25.816 337 212 10 216 541 129 438 3.35e-20 95.1
MS.gene32221.t1 AT3G24190 26.481 287 184 7 224 509 203 463 3.93e-20 95.5
MS.gene32221.t1 AT4G31390 27.305 282 176 7 224 500 178 435 6.88e-20 94.4
MS.gene32221.t1 AT2G39190 27.055 292 175 7 215 490 201 470 1.59e-18 90.5
MS.gene32221.t1 AT5G24810 26.741 359 188 14 190 509 60 382 6.66e-18 88.6
MS.gene32221.t1 AT2G40090 25.325 308 207 9 222 520 98 391 1.78e-17 86.3
MS.gene32221.t1 AT5G50330 28.571 301 153 9 224 506 76 332 7.45e-17 84.0
MS.gene32221.t1 AT2G39190 28.743 167 108 3 215 380 201 357 9.21e-13 70.9

Find 150 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACCATTTGCAGATTATTT+TGG 0.167715 4.4:-22508522 MS.gene32221:CDS
AGTTCATCCCTGCACTTAAT+TGG 0.229978 4.4:-22508086 MS.gene32221:CDS
TGGAAAAGGCATTTACCTTT+TGG 0.276940 4.4:-22505730 MS.gene32221:CDS
GTAGAAGGCCTGGTCTTGTT+AGG 0.291072 4.4:-22508591 MS.gene32221:CDS
CAGGTCCGGCATTCATAAAA+TGG 0.303106 4.4:-22508446 MS.gene32221:CDS
ACTTGTGCATCCCGCAGTTT+TGG 0.315016 4.4:-22507920 MS.gene32221:CDS
AAAACTATGCTCAGGGGCTT+TGG 0.338841 4.4:+22508353 None:intergenic
TCAAGCAGTGAGCCTGGCTT+TGG 0.346858 4.4:-22508778 MS.gene32221:CDS
TGCCATCAATATTAACTCTA+TGG 0.356498 4.4:+22505639 None:intergenic
TTATTTACAATTTCCGTCAA+TGG 0.356698 4.4:-22507975 MS.gene32221:CDS
TCAATATAAGCCTTCGGATC+AGG 0.360819 4.4:+22506492 None:intergenic
TTGGTGGAAACATATGAAAA+TGG 0.369350 4.4:-22507901 MS.gene32221:CDS
TCCCTGCACTTAATTGGTTA+AGG 0.369612 4.4:-22508080 MS.gene32221:CDS
TTTGCCTTTATTGTGGTAGA+AGG 0.371174 4.4:-22508606 MS.gene32221:CDS
TATTCTTCCAATGGTTATAG+TGG 0.376964 4.4:-22508825 MS.gene32221:CDS
CGCCATAGAGTTAATATTGA+TGG 0.387144 4.4:-22505641 MS.gene32221:CDS
CATTATGTTGATGAGCTTCA+AGG 0.387369 4.4:-22507865 MS.gene32221:CDS
ATGGGCTTTGCTTGCTGACC+AGG 0.400663 4.4:+22508198 None:intergenic
AAAAGCGCTCTTGCTCATAT+TGG 0.402007 4.4:-22507829 MS.gene32221:CDS
AAAACTATAGAAAGCGCATT+TGG 0.402533 4.4:-22508318 MS.gene32221:CDS
TGTCCTGCATCGGTTGATAT+TGG 0.406194 4.4:+22509013 None:intergenic
ACAAGTGGATAAACAGGCTT+AGG 0.406606 4.4:+22507937 None:intergenic
AGCAATTACTTGAGAAAGTT+AGG 0.407683 4.4:-22505664 MS.gene32221:CDS
AATTGCTCCATGCACTCGGC+TGG 0.409786 4.4:+22505680 None:intergenic
AGGTCCGGCATTCATAAAAT+GGG 0.412415 4.4:-22508445 MS.gene32221:CDS
AACAAGAACGAGCCAAAGCC+AGG 0.418604 4.4:+22508766 None:intergenic
GAAGAATTGCCTGTTGCATC+TGG 0.432722 4.4:-22508264 MS.gene32221:CDS
GGATGCACAAGTGGATAAAC+AGG 0.432971 4.4:+22507931 None:intergenic
CAGGCCTTCTACCACAATAA+AGG 0.433283 4.4:+22508602 None:intergenic
CTTTATTGTGGTAGAAGGCC+TGG 0.436081 4.4:-22508601 MS.gene32221:CDS
CACATAAATCTCAAATTGCT+TGG 0.437682 4.4:-22508866 MS.gene32221:CDS
ATGGATTCTAAGAATAGATT+CGG 0.438036 4.4:+22509088 None:intergenic
TGCACTTAATTGGTTAAGGT+TGG 0.453314 4.4:-22508076 MS.gene32221:CDS
TGGCAGCGCAAGCTTGATCC+CGG 0.453902 4.4:-22505374 MS.gene32221:CDS
GATTGAACTACATCCTTCAC+TGG 0.455273 4.4:+22508971 None:intergenic
TGTTAGTTGCAGTGAAGGTC+AGG 0.455861 4.4:-22508176 MS.gene32221:CDS
GTCCTGCATCGGTTGATATT+GGG 0.456084 4.4:+22509014 None:intergenic
ATTCATGCAGACATGCATCC+TGG 0.462391 4.4:-22506723 MS.gene32221:CDS
GCACTCATACACAAAGCATT+TGG 0.464871 4.4:+22508657 None:intergenic
TTTCTAACAGGAAGTGGAAA+AGG 0.472158 4.4:-22505744 MS.gene32221:intron
TACACTAGAAAGAGCAGGTC+CGG 0.473539 4.4:-22508460 MS.gene32221:CDS
CAGCAAAATCAGTCAGATGC+AGG 0.476405 4.4:-22508693 MS.gene32221:CDS
GTGTAGCTAAAACTATGCTC+AGG 0.476963 4.4:+22508345 None:intergenic
TAGCGATCGGGTAAATTTAG+TGG 0.477735 4.4:-22506595 MS.gene32221:CDS
GACCTTCACTGCAACTAACA+TGG 0.486515 4.4:+22508179 None:intergenic
GATTGCTCAAGCAGTGAGCC+TGG 0.489155 4.4:-22508784 MS.gene32221:CDS
GGCATGACGGCTGAACTCTC+TGG 0.490763 4.4:-22506618 MS.gene32221:CDS
ACTCGAACAAGAATATTTCC+AGG 0.491498 4.4:+22506705 None:intergenic
GCAGTGAAGGTCAGGCATCC+TGG 0.494938 4.4:-22508168 MS.gene32221:CDS
AACCTTAACCAATTAAGTGC+AGG 0.495535 4.4:+22508078 None:intergenic
CAATATAAGCCTTCGGATCA+GGG 0.495816 4.4:+22506493 None:intergenic
TGTCTCAAGTTGACCTTGCA+AGG 0.500238 4.4:-22508020 MS.gene32221:CDS
GCAATACTTCCAGATGCAAC+AGG 0.505670 4.4:+22508255 None:intergenic
GGGGTCAGTGGGCGGCTACA+CGG 0.505755 4.4:-22508425 MS.gene32221:CDS
ATTACCTCAATATAAGCCTT+CGG 0.506641 4.4:+22506486 None:intergenic
TTATGCTTGGACACATTGGG+AGG 0.510559 4.4:-22509115 MS.gene32221:CDS
TGTTCGAGTGCCTCAGAGCA+AGG 0.513395 4.4:-22506691 MS.gene32221:CDS
TGAACTCTCTGGTAGCGATC+GGG 0.513964 4.4:-22506607 MS.gene32221:CDS
CATTGCTGCTTAGAGCTGAC+TGG 0.514309 4.4:-22505325 MS.gene32221:CDS
TTAAAGACGCACGATGCACT+TGG 0.516442 4.4:+22508232 None:intergenic
AATGAGCAGCTTCCCTTGCA+AGG 0.519250 4.4:+22508007 None:intergenic
CTGTGTGTCAGCAGAAATGC+AGG 0.519862 4.4:-22509053 MS.gene32221:CDS
GGGCCAAAATAATCTGCAAA+TGG 0.520579 4.4:+22508519 None:intergenic
TGTCAGCAGAAATGCAGGTT+TGG 0.521589 4.4:-22509048 MS.gene32221:CDS
AAAGCTGTTGCCCGCAGAGA+TGG 0.526628 4.4:-22506564 MS.gene32221:CDS
ATATCTTGAGGAAATAGATC+AGG 0.530009 4.4:+22508402 None:intergenic
CACCCAATATCAACCGATGC+AGG 0.531406 4.4:-22509016 MS.gene32221:CDS
GCCCATGTTAGTTGCAGTGA+AGG 0.532016 4.4:-22508181 MS.gene32221:CDS
TAGAAGGCCTGGTCTTGTTA+GGG 0.534001 4.4:-22508590 MS.gene32221:CDS
TATGTTTCCACCAAAACTGC+GGG 0.536343 4.4:+22507910 None:intergenic
TACTGTCATGGTGACCACCT+TGG 0.536843 4.4:-22505609 MS.gene32221:CDS
ACCTTCTCGAGTTCCCCAAA+AGG 0.537128 4.4:+22505715 None:intergenic
TGAGGCTTTGATTTGAAGAA+CGG 0.538324 4.4:+22506660 None:intergenic
GTTCAGGAAAATGTGGCTCC+AGG 0.540062 4.4:-22508493 MS.gene32221:CDS
ATTCATAAAATGGGGTCAGT+GGG 0.541035 4.4:-22508436 MS.gene32221:CDS
CATAAAATATATTCTTCCAA+TGG 0.541989 4.4:-22508834 MS.gene32221:CDS
GGTAGATCCACTATAACCAT+TGG 0.542434 4.4:+22508818 None:intergenic
CTGAACTCTCTGGTAGCGAT+CGG 0.542589 4.4:-22506608 MS.gene32221:CDS
ACCTTCACTGCAACTAACAT+GGG 0.543161 4.4:+22508180 None:intergenic
AATGGTGCCATCAGTATGCT+TGG 0.544641 4.4:+22508537 None:intergenic
AGCGGTCCGACCATCTCTGC+GGG 0.550823 4.4:+22506554 None:intergenic
GAAGGTCAGGCATCCTGGTG+TGG 0.551469 4.4:-22508163 MS.gene32221:CDS
AAGTAATTGCTCCATGCACT+CGG 0.552365 4.4:+22505676 None:intergenic
AAGACACGTCCCTCCATTGA+CGG 0.554021 4.4:+22507962 None:intergenic
GTTTGCATTTGCCTTTATTG+TGG 0.554906 4.4:-22508613 MS.gene32221:CDS
CAGTATAAGTTTCCCAGTGA+AGG 0.559877 4.4:-22508984 MS.gene32221:CDS
GACGGAAATTGTAAATAAAG+CGG 0.560686 4.4:+22507980 None:intergenic
GATGAGCTTCAAGGACATGA+AGG 0.561596 4.4:-22507856 MS.gene32221:CDS
TACACAAAGCATTTGGTGAA+GGG 0.561670 4.4:+22508664 None:intergenic
CTGATAGATTCTCCCACACC+AGG 0.564921 4.4:+22508150 None:intergenic
AAGAATTACCTCTAAAACCA+AGG 0.567303 4.4:+22505592 None:intergenic
ATTGCTCCATGCACTCGGCT+GGG 0.575831 4.4:+22505681 None:intergenic
TTTACAATTTCCGTCAATGG+AGG 0.577225 4.4:-22507972 MS.gene32221:CDS
TGTAGCTAAAACTATGCTCA+GGG 0.577705 4.4:+22508346 None:intergenic
CATTCATAAAATGGGGTCAG+TGG 0.577994 4.4:-22508437 MS.gene32221:CDS
TATGCTTGGACACATTGGGA+GGG 0.577995 4.4:-22509114 MS.gene32221:CDS
TCTTTAAAATATCGTTACCC+TGG 0.578971 4.4:-22508216 MS.gene32221:CDS
ACAGAACTGCCCTGATCCGA+AGG 0.579301 4.4:-22506502 MS.gene32221:CDS
CAAGTGGATAAACAGGCTTA+GGG 0.583288 4.4:+22507938 None:intergenic
GGCAGCGCAAGCTTGATCCC+GGG 0.583620 4.4:-22505373 MS.gene32221:CDS
AAGGTCAGGCATCCTGGTGT+GGG 0.584830 4.4:-22508162 MS.gene32221:CDS
TGTCTCCATCACATTATACC+CGG 0.586292 4.4:+22505355 None:intergenic
CTAGTGTACGATGAACAACC+TGG 0.586872 4.4:+22508475 None:intergenic
TTACAATTTCCGTCAATGGA+GGG 0.587427 4.4:-22507971 MS.gene32221:CDS
CATAAAATGGGGTCAGTGGG+CGG 0.590431 4.4:-22508433 MS.gene32221:CDS
TGGCAATGTTTGTACTGTCA+TGG 0.591925 4.4:-22505621 MS.gene32221:CDS
GTGTGGGAGAATCTATCAGA+AGG 0.592449 4.4:-22508146 MS.gene32221:CDS
ACCTTAACCAATTAAGTGCA+GGG 0.592733 4.4:+22508079 None:intergenic
GGTTCACCCAGCCGAGTGCA+TGG 0.595900 4.4:-22505687 MS.gene32221:CDS
ATTGCTGCTTAGAGCTGACT+GGG 0.597161 4.4:-22505324 MS.gene32221:CDS
TGCATGTGGAGAGCTAGCAT+TGG 0.597506 4.4:-22508718 MS.gene32221:CDS
ATTCTCTCCAAGCATACTGA+TGG 0.598290 4.4:-22508544 MS.gene32221:CDS
TGATCCCGGGTATAATGTGA+TGG 0.598520 4.4:-22505360 MS.gene32221:CDS
TAAAGACGCACGATGCACTT+GGG 0.599670 4.4:+22508233 None:intergenic
ATACACAAAGCATTTGGTGA+AGG 0.601176 4.4:+22508663 None:intergenic
AGCTTAGTACATATATCTTG+AGG 0.603331 4.4:+22508390 None:intergenic
GCCCCGAGTTCAGGAAAATG+TGG 0.603655 4.4:-22508500 MS.gene32221:CDS
TAGCTCTCCCTAACAAGACC+AGG 0.606113 4.4:+22508583 None:intergenic
ACTGCATATATGTCCTGCAT+CGG 0.608798 4.4:+22509003 None:intergenic
CCGCTGCTGAGTGCACACTC+AGG 0.611189 4.4:-22506536 MS.gene32221:CDS
TGATCCGAAGGCTTATATTG+AGG 0.613318 4.4:-22506490 MS.gene32221:intron
CATCGTACACTAGAAAGAGC+AGG 0.614986 4.4:-22508465 MS.gene32221:CDS
GCTAGAGAAAACTTTCACAA+AGG 0.615426 4.4:-22508936 MS.gene32221:CDS
AAAACTGCGGGATGCACAAG+TGG 0.616371 4.4:+22507922 None:intergenic
AGCAAAATCAGTCAGATGCA+GGG 0.618278 4.4:-22508692 MS.gene32221:CDS
CAGAGTCTGATGGCCCCTTG+AGG 0.619099 4.4:-22505278 None:intergenic
TTACACAATTCAGAGTCTGA+TGG 0.621107 4.4:-22505288 MS.gene32221:CDS
TTTCCTCGATGTAGGCATGA+CGG 0.621991 4.4:-22506631 MS.gene32221:CDS
ATGAGCTTCAAGGACATGAA+GGG 0.622640 4.4:-22507855 MS.gene32221:CDS
GTCTCAAGTTGACCTTGCAA+GGG 0.622912 4.4:-22508019 MS.gene32221:CDS
TGGGCTTTGCTTGCTGACCA+GGG 0.624719 4.4:+22508199 None:intergenic
AAAGAAAGAACCTCCTCAAG+GGG 0.634321 4.4:+22505265 None:intergenic
ATATGTTTCCACCAAAACTG+CGG 0.642456 4.4:+22507909 None:intergenic
GTCTCCATCACATTATACCC+GGG 0.643086 4.4:+22505356 None:intergenic
TTGTGTATGAGTGCACAAGA+TGG 0.646764 4.4:-22508648 MS.gene32221:CDS
CCTGAGTGTGCACTCAGCAG+CGG 0.653822 4.4:+22506536 None:intergenic
GGTTTGCGAGCCTTGCTCTG+AGG 0.654052 4.4:+22506681 None:intergenic
CTGTTGCCCGCAGAGATGGT+CGG 0.657647 4.4:-22506560 MS.gene32221:CDS
AAGGATGTAGTTCAATCATG+TGG 0.658916 4.4:-22508965 MS.gene32221:CDS
CAGCGGTCCGACCATCTCTG+CGG 0.665411 4.4:+22506553 None:intergenic
TGTAGTTCAATCATGTGGCG+AGG 0.668343 4.4:-22508960 MS.gene32221:CDS
ATTGAACTACATCCTTCACT+GGG 0.674087 4.4:+22508972 None:intergenic
GGTCCGGCATTCATAAAATG+GGG 0.675314 4.4:-22508444 MS.gene32221:CDS
TGTGGGAGAATCTATCAGAA+GGG 0.684154 4.4:-22508145 MS.gene32221:CDS
TTGAGCAATCATATGTACAG+AGG 0.703226 4.4:+22508797 None:intergenic
ACATCGAGGAAAATTACATG+AGG 0.705947 4.4:+22506642 None:intergenic
GGGAACTCGAGAAGGTGACG+CGG 0.730457 4.4:-22505708 MS.gene32221:CDS
AATTACCTCTAAAACCAAGG+TGG 0.739212 4.4:+22505595 None:intergenic
CAGCCGTCATGCCTACATCG+AGG 0.745012 4.4:+22506628 None:intergenic
GTAGCTAAAACTATGCTCAG+GGG 0.756366 4.4:+22508347 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGAGATACATATTTAA+TGG + chr4.4:22507059-22507078 None:intergenic 15.0%
!! AAAAATTAAATTTCAGCATA+AGG - chr4.4:22507383-22507402 MS.gene32221:intron 15.0%
!! CAATATATATATAACAAGAA+AGG + chr4.4:22506644-22506663 None:intergenic 15.0%
!! TAAAATCTAATTTATGCTTT+CGG + chr4.4:22508899-22508918 None:intergenic 15.0%
!!! TTATTATGTATATTAGCTTA+TGG - chr4.4:22507934-22507953 MS.gene32221:CDS 15.0%
!!! TTCTTGTTATATATATATTG+TGG - chr4.4:22506644-22506663 MS.gene32221:CDS 15.0%
!! CATAAAATATATTCTTCCAA+TGG - chr4.4:22505559-22505578 MS.gene32221:intron 20.0%
!! TAATTTACATTGTTAGTCAA+AGG - chr4.4:22507078-22507097 MS.gene32221:intron 20.0%
!! TACAAATATAAGATTTAGAG+TGG + chr4.4:22508223-22508242 None:intergenic 20.0%
!! TGTTATATATATATTGTGGT+TGG - chr4.4:22506648-22506667 MS.gene32221:CDS 20.0%
!! TTAAATTTAATCCTGATCAT+CGG + chr4.4:22508121-22508140 None:intergenic 20.0%
!! TTAATAATTACCAATGTAGA+TGG + chr4.4:22508949-22508968 None:intergenic 20.0%
!! TTACAAGATTGAATTTCATA+AGG + chr4.4:22507360-22507379 None:intergenic 20.0%
!! TTCCAAAAACTAAAGTTATT+AGG + chr4.4:22507280-22507299 None:intergenic 20.0%
!!! AAGTATTGTAATAAAAACTG+TGG + chr4.4:22506972-22506991 None:intergenic 20.0%
!!! ACAAATATAAGATTTAGAGT+GGG + chr4.4:22508222-22508241 None:intergenic 20.0%
!!! TGGTGAATATATAATTTGTA+GGG - chr4.4:22508995-22509014 MS.gene32221:CDS 20.0%
!!! TTGGTGAATATATAATTTGT+AGG - chr4.4:22508994-22509013 MS.gene32221:CDS 20.0%
! AGATATATAGCTATTGTTAG+TGG - chr4.4:22506784-22506803 MS.gene32221:intron 25.0%
! ATACAATTAATATACGCTAC+TGG - chr4.4:22508440-22508459 MS.gene32221:CDS 25.0%
! GATGATCAGGATTAAATTTA+AGG - chr4.4:22508120-22508139 MS.gene32221:CDS 25.0%
! GTAGCGTATATTAATTGTAT+TGG + chr4.4:22508440-22508459 None:intergenic 25.0%
! TACAATTAATATACGCTACT+GGG - chr4.4:22508441-22508460 MS.gene32221:CDS 25.0%
! TCCATATTAACTTCATTTCA+GGG + chr4.4:22506758-22506777 None:intergenic 25.0%
! TGTTGGCAAAAATATTAACA+GGG + chr4.4:22507159-22507178 None:intergenic 25.0%
! TTATAAAAAGCCAATGACAA+TGG + chr4.4:22508526-22508545 None:intergenic 25.0%
! TTATTTACAATTTCCGTCAA+TGG - chr4.4:22506418-22506437 MS.gene32221:intron 25.0%
! TTGTTGGCAAAAATATTAAC+AGG + chr4.4:22507160-22507179 None:intergenic 25.0%
!! ATGGATTCTAAGAATAGATT+CGG + chr4.4:22505308-22505327 None:intergenic 25.0%
!! ATTTGTTGATCAAACAAACT+TGG + chr4.4:22506868-22506887 None:intergenic 25.0%
!! CACAAACACAATAGAATTTT+TGG - chr4.4:22508387-22508406 MS.gene32221:CDS 25.0%
!! CATTGGTAATTATTAAAAGC+TGG - chr4.4:22508953-22508972 MS.gene32221:CDS 25.0%
!! GAATATATAATTTGTAGGGT+TGG - chr4.4:22508999-22509018 MS.gene32221:CDS 25.0%
!! TATGTATATTAGCTTATGGT+TGG - chr4.4:22507938-22507957 MS.gene32221:CDS 25.0%
!! TTTCGCTATTATCAATTTTG+TGG - chr4.4:22506272-22506291 MS.gene32221:intron 25.0%
!!! CACCTAATAACTTTAGTTTT+TGG - chr4.4:22507275-22507294 MS.gene32221:intron 25.0%
!!! TGTGAGTCATTTTATACATT+TGG - chr4.4:22508614-22508633 MS.gene32221:CDS 25.0%
!!! TTATACATTTGGTTTTCTCT+TGG - chr4.4:22508625-22508644 MS.gene32221:CDS 25.0%
AAACATAAACTCTGTTACAG+TGG + chr4.4:22508837-22508856 None:intergenic 30.0%
AAGTGAAGTTCCAAAAAGAA+CGG + chr4.4:22505483-22505502 None:intergenic 30.0%
AATTACCAATGTAGATGGTT+TGG + chr4.4:22508944-22508963 None:intergenic 30.0%
AATTCTATTGTGTTTGTGTG+TGG + chr4.4:22508385-22508404 None:intergenic 30.0%
AGCAATTACTTGAGAAAGTT+AGG - chr4.4:22508729-22508748 MS.gene32221:CDS 30.0%
AGCTTAGTACATATATCTTG+AGG + chr4.4:22506006-22506025 None:intergenic 30.0%
AGGTACTATAATAGATGACA+AGG + chr4.4:22507242-22507261 None:intergenic 30.0%
ATAGTATCCGTTTAGATATG+TGG + chr4.4:22508300-22508319 None:intergenic 30.0%
ATATCTTGAGGAAATAGATC+AGG + chr4.4:22505994-22506013 None:intergenic 30.0%
ATTACCTCAATATAAGCCTT+CGG + chr4.4:22507910-22507929 None:intergenic 30.0%
ATTCAAAACATACCAGTAGA+TGG + chr4.4:22508175-22508194 None:intergenic 30.0%
ATTCTATTGTGTTTGTGTGT+GGG + chr4.4:22508384-22508403 None:intergenic 30.0%
CACATAAATCTCAAATTGCT+TGG - chr4.4:22505527-22505546 MS.gene32221:intron 30.0%
CATAGTGTAGAAAAATGTAC+AGG + chr4.4:22507010-22507029 None:intergenic 30.0%
CTCCATATTAACTTCATTTC+AGG + chr4.4:22506759-22506778 None:intergenic 30.0%
GACGGAAATTGTAAATAAAG+CGG + chr4.4:22506416-22506435 None:intergenic 30.0%
TATATAGCTATTGTTAGTGG+TGG - chr4.4:22506787-22506806 MS.gene32221:intron 30.0%
TATTCTTCCAATGGTTATAG+TGG - chr4.4:22505568-22505587 MS.gene32221:intron 30.0%
TCAAACAAACTTGGTTAAGT+AGG + chr4.4:22506859-22506878 None:intergenic 30.0%
TGCCATCAATATTAACTCTA+TGG + chr4.4:22508757-22508776 None:intergenic 30.0%
TTCAAGCAACTTAAGTAAGT+AGG - chr4.4:22507493-22507512 MS.gene32221:intron 30.0%
TTGACATGCATGGAAAATAA+TGG - chr4.4:22508866-22508885 MS.gene32221:CDS 30.0%
TTGGAAGCTAAACAATTGTA+TGG + chr4.4:22507615-22507634 None:intergenic 30.0%
TTGGTGGAAACATATGAAAA+TGG - chr4.4:22506492-22506511 MS.gene32221:CDS 30.0%
TTTCGTCACTTCAAATTTCA+GGG + chr4.4:22506895-22506914 None:intergenic 30.0%
! AAAACTATAGAAAGCGCATT+TGG - chr4.4:22506075-22506094 MS.gene32221:intron 30.0%
! AATAGAAGTTGATTATCCAG+TGG + chr4.4:22507036-22507055 None:intergenic 30.0%
! ATAGAAGTTGATTATCCAGT+GGG + chr4.4:22507035-22507054 None:intergenic 30.0%
! CAAGTTTAAAGCCATCAAAA+GGG + chr4.4:22507588-22507607 None:intergenic 30.0%
! TATTTTGTGATTGACATGCA+TGG - chr4.4:22508856-22508875 MS.gene32221:CDS 30.0%
! TCAAGTTTAAAGCCATCAAA+AGG + chr4.4:22507589-22507608 None:intergenic 30.0%
! TTTCATCCAAAAAGTTTGCT+GGG - chr4.4:22507562-22507581 MS.gene32221:intron 30.0%
! TTTGTAATGTCTTGTTAGTC+TGG - chr4.4:22506928-22506947 MS.gene32221:intron 30.0%
! TTTTCGTCACTTCAAATTTC+AGG + chr4.4:22506896-22506915 None:intergenic 30.0%
!! AAGAATTACCTCTAAAACCA+AGG + chr4.4:22508804-22508823 None:intergenic 30.0%
!! AGAGCATAGTTGATAATTGT+TGG + chr4.4:22507176-22507195 None:intergenic 30.0%
!! TCTTTAAAATATCGTTACCC+TGG - chr4.4:22506177-22506196 MS.gene32221:intron 30.0%
!! TTTTCTCTTGGTTTTCTAAC+AGG - chr4.4:22508637-22508656 MS.gene32221:CDS 30.0%
!!! AACTTTAGTTTTTGGAACAG+TGG - chr4.4:22507283-22507302 MS.gene32221:intron 30.0%
!!! ACTTTAGTTTTTGGAACAGT+GGG - chr4.4:22507284-22507303 MS.gene32221:intron 30.0%
AACCTTAACCAATTAAGTGC+AGG + chr4.4:22506318-22506337 None:intergenic 35.0%
AAGGATGTAGTTCAATCATG+TGG - chr4.4:22505428-22505447 MS.gene32221:intron 35.0%
AATTTCAGCATAAGGTGAGA+TGG - chr4.4:22507391-22507410 MS.gene32221:intron 35.0%
ACATCGAGGAAAATTACATG+AGG + chr4.4:22507754-22507773 None:intergenic 35.0%
ACCTTAACCAATTAAGTGCA+GGG + chr4.4:22506317-22506336 None:intergenic 35.0%
ACTCGAACAAGAATATTTCC+AGG + chr4.4:22507691-22507710 None:intergenic 35.0%
ATATGTTTCCACCAAAACTG+CGG + chr4.4:22506487-22506506 None:intergenic 35.0%
ATTCATAAAATGGGGTCAGT+GGG - chr4.4:22505957-22505976 MS.gene32221:intron 35.0%
ATTGAACTACATCCTTCACT+GGG + chr4.4:22505424-22505443 None:intergenic 35.0%
ATTTCAGCATAAGGTGAGAT+GGG - chr4.4:22507392-22507411 MS.gene32221:intron 35.0%
CATACGGATCTATTTCACTT+TGG - chr4.4:22508485-22508504 MS.gene32221:CDS 35.0%
CATGCAAACGCAAAAATACT+AGG + chr4.4:22505661-22505680 None:intergenic 35.0%
CGCCATAGAGTTAATATTGA+TGG - chr4.4:22508752-22508771 MS.gene32221:CDS 35.0%
CTTTCATCCAAAAAGTTTGC+TGG - chr4.4:22507561-22507580 MS.gene32221:intron 35.0%
GCAACTTAAGTAAGTAGGAT+TGG - chr4.4:22507498-22507517 MS.gene32221:intron 35.0%
GCATGGAAAATAATGGTGAA+AGG - chr4.4:22508873-22508892 MS.gene32221:CDS 35.0%
GCCCTGAAATGAAGTTAATA+TGG - chr4.4:22506754-22506773 MS.gene32221:intron 35.0%
GCTAGAGAAAACTTTCACAA+AGG - chr4.4:22505457-22505476 MS.gene32221:intron 35.0%
GTCTGGTACAAAAAACAAAG+TGG - chr4.4:22506945-22506964 MS.gene32221:intron 35.0%
GTTTGCATTTGCCTTTATTG+TGG - chr4.4:22505780-22505799 MS.gene32221:intron 35.0%
TACAATCATAACCGATGATC+AGG - chr4.4:22508107-22508126 MS.gene32221:CDS 35.0%
TGCACTTAATTGGTTAAGGT+TGG - chr4.4:22506317-22506336 MS.gene32221:intron 35.0%
TGTAGCTAAAACTATGCTCA+GGG + chr4.4:22506050-22506069 None:intergenic 35.0%
TTACAATTTCCGTCAATGGA+GGG - chr4.4:22506422-22506441 MS.gene32221:intron 35.0%
TTACACAATTCAGAGTCTGA+TGG - chr4.4:22509105-22509124 MS.gene32221:CDS 35.0%
TTAGAACCTTTATGACCAAG+TGG - chr4.4:22507321-22507340 MS.gene32221:intron 35.0%
TTCGTCACTTCAAATTTCAG+GGG + chr4.4:22506894-22506913 None:intergenic 35.0%
TTGAGCAATCATATGTACAG+AGG + chr4.4:22505599-22505618 None:intergenic 35.0%
TTTACAATTTCCGTCAATGG+AGG - chr4.4:22506421-22506440 MS.gene32221:intron 35.0%
TTTCTAACAGGAAGTGGAAA+AGG - chr4.4:22508649-22508668 MS.gene32221:CDS 35.0%
TTTGCCTTTATTGTGGTAGA+AGG - chr4.4:22505787-22505806 MS.gene32221:intron 35.0%
! AAGGTTCTAATACCATGTCA+AGG + chr4.4:22507311-22507330 None:intergenic 35.0%
! ATACACAAAGCATTTGGTGA+AGG + chr4.4:22505733-22505752 None:intergenic 35.0%
! CATGTAATTTTCCTCGATGT+AGG - chr4.4:22507754-22507773 MS.gene32221:intron 35.0%
! CATTATGTTGATGAGCTTCA+AGG - chr4.4:22506528-22506547 MS.gene32221:CDS 35.0%
! CTTTGTGTGTTGTGTCATTA+AGG - chr4.4:22506710-22506729 MS.gene32221:CDS 35.0%
! GCACCATTTGCAGATTATTT+TGG - chr4.4:22505871-22505890 MS.gene32221:intron 35.0%
! GTGTGACTGACTTTATACAT+TGG - chr4.4:22508975-22508994 MS.gene32221:CDS 35.0%
! GTTGGATGAGTTTTACATGA+AGG - chr4.4:22507956-22507975 MS.gene32221:CDS 35.0%
! TACACAAAGCATTTGGTGAA+GGG + chr4.4:22505732-22505751 None:intergenic 35.0%
!! AATTACCTCTAAAACCAAGG+TGG + chr4.4:22508801-22508820 None:intergenic 35.0%
!! AGTATTTTTGCGTTTGCATG+TGG - chr4.4:22505661-22505680 MS.gene32221:CDS 35.0%
!! CACACAGATATCTGCTTTTA+TGG + chr4.4:22505327-22505346 None:intergenic 35.0%
!! TGAGGCTTTGATTTGAAGAA+CGG + chr4.4:22507736-22507755 None:intergenic 35.0%
!!! GAAAAGGCATTTACCTTTTG+GGG - chr4.4:22508665-22508684 MS.gene32221:CDS 35.0%
!!! GGAAAAGGCATTTACCTTTT+GGG - chr4.4:22508664-22508683 MS.gene32221:CDS 35.0%
!!! TGGAAAAGGCATTTACCTTT+TGG - chr4.4:22508663-22508682 MS.gene32221:CDS 35.0%
AAAAGCCAATGACAATGGCT+AGG + chr4.4:22508521-22508540 None:intergenic 40.0%
AAGTAATTGCTCCATGCACT+CGG + chr4.4:22508720-22508739 None:intergenic 40.0%
ACAAGTGGATAAACAGGCTT+AGG + chr4.4:22506459-22506478 None:intergenic 40.0%
ACCTTCACTGCAACTAACAT+GGG + chr4.4:22506216-22506235 None:intergenic 40.0%
ACTGCATATATGTCCTGCAT+CGG + chr4.4:22505393-22505412 None:intergenic 40.0%
ACTTGTCCCTACTAAGTCTA+GGG + chr4.4:22506824-22506843 None:intergenic 40.0%
AGCAAAATCAGTCAGATGCA+GGG - chr4.4:22505701-22505720 MS.gene32221:CDS 40.0%
AGGTCCGGCATTCATAAAAT+GGG - chr4.4:22505948-22505967 MS.gene32221:intron 40.0%
AGTGAAATAGATCCGTATGC+AGG + chr4.4:22508484-22508503 None:intergenic 40.0%
AGTTCATCCCTGCACTTAAT+TGG - chr4.4:22506307-22506326 MS.gene32221:intron 40.0%
ATCAGGCTAAAGCATGTGAA+AGG + chr4.4:22508352-22508371 None:intergenic 40.0%
ATGACTCACAATCCTAACCA+AGG + chr4.4:22508604-22508623 None:intergenic 40.0%
ATGAGCTTCAAGGACATGAA+GGG - chr4.4:22506538-22506557 MS.gene32221:CDS 40.0%
ATGCACCAAACCATCTACAT+TGG - chr4.4:22508936-22508955 MS.gene32221:CDS 40.0%
ATTCTCTCCAAGCATACTGA+TGG - chr4.4:22505849-22505868 MS.gene32221:intron 40.0%
CAAGTGGATAAACAGGCTTA+GGG + chr4.4:22506458-22506477 None:intergenic 40.0%
CAATATAAGCCTTCGGATCA+GGG + chr4.4:22507903-22507922 None:intergenic 40.0%
CAGTATAAGTTTCCCAGTGA+AGG - chr4.4:22505409-22505428 MS.gene32221:intron 40.0%
CATTCATAAAATGGGGTCAG+TGG - chr4.4:22505956-22505975 MS.gene32221:intron 40.0%
GATTGAACTACATCCTTCAC+TGG + chr4.4:22505425-22505444 None:intergenic 40.0%
GGATTGAATTCTTGACCACT+TGG + chr4.4:22507339-22507358 None:intergenic 40.0%
GGGCCAAAATAATCTGCAAA+TGG + chr4.4:22505877-22505896 None:intergenic 40.0%
GGTAGATCCACTATAACCAT+TGG + chr4.4:22505578-22505597 None:intergenic 40.0%
GTAAAGTCTAAGTACGAGGA+AGG + chr4.4:22507212-22507231 None:intergenic 40.0%
GTAGCTAAAACTATGCTCAG+GGG + chr4.4:22506049-22506068 None:intergenic 40.0%
GTGAAATAGATCCGTATGCA+GGG + chr4.4:22508483-22508502 None:intergenic 40.0%
GTGTAGCTAAAACTATGCTC+AGG + chr4.4:22506051-22506070 None:intergenic 40.0%
TACTTGTCCCTACTAAGTCT+AGG + chr4.4:22506825-22506844 None:intergenic 40.0%
TAGCGATCGGGTAAATTTAG+TGG - chr4.4:22507798-22507817 MS.gene32221:intron 40.0%
TATGTTTCCACCAAAACTGC+GGG + chr4.4:22506486-22506505 None:intergenic 40.0%
TCAATATAAGCCTTCGGATC+AGG + chr4.4:22507904-22507923 None:intergenic 40.0%
TCCCTGCACTTAATTGGTTA+AGG - chr4.4:22506313-22506332 MS.gene32221:intron 40.0%
TCTTGACCACTTGGTCATAA+AGG + chr4.4:22507330-22507349 None:intergenic 40.0%
TGATCCGAAGGCTTATATTG+AGG - chr4.4:22507903-22507922 MS.gene32221:CDS 40.0%
TGCTGCACCACATATCTAAA+CGG - chr4.4:22508290-22508309 MS.gene32221:CDS 40.0%
TGGCAATGTTTGTACTGTCA+TGG - chr4.4:22508772-22508791 MS.gene32221:CDS 40.0%
TGTCTCCATCACATTATACC+CGG + chr4.4:22509041-22509060 None:intergenic 40.0%
TGTGGGAGAATCTATCAGAA+GGG - chr4.4:22506248-22506267 MS.gene32221:intron 40.0%
TGTGTTTGTGTGTGGGTATT+GGG + chr4.4:22508377-22508396 None:intergenic 40.0%
TTAGGTGAGCTGTATCTAAC+AGG + chr4.4:22507262-22507281 None:intergenic 40.0%
TTGTGTATGAGTGCACAAGA+TGG - chr4.4:22505745-22505764 MS.gene32221:intron 40.0%
TTGTGTTTGTGTGTGGGTAT+TGG + chr4.4:22508378-22508397 None:intergenic 40.0%
TTTCTACACTATGAACCCAC+TGG - chr4.4:22507017-22507036 MS.gene32221:intron 40.0%
! AGATTTAGAGTGGGTGCAAT+AGG + chr4.4:22508213-22508232 None:intergenic 40.0%
! GCACTCATACACAAAGCATT+TGG + chr4.4:22505739-22505758 None:intergenic 40.0%
! TGACCCCATTTTATGAATGC+CGG + chr4.4:22505955-22505974 None:intergenic 40.0%
! TTCACAAAGGCCGTTCTTTT+TGG - chr4.4:22505470-22505489 MS.gene32221:intron 40.0%
! TTTTGCCTAGCCATTGTCAT+TGG - chr4.4:22508513-22508532 MS.gene32221:CDS 40.0%
!! AAAAGCGCTCTTGCTCATAT+TGG - chr4.4:22506564-22506583 MS.gene32221:CDS 40.0%
!! CCCATATTTTAAGCCCCAAA+GGG - chr4.4:22507426-22507445 MS.gene32221:intron 40.0%
!! CCCTTTGGGGCTTAAAATAT+GGG + chr4.4:22507429-22507448 None:intergenic 40.0%
!! CTGGTATGTTTTGAATCACG+CGG - chr4.4:22508179-22508198 MS.gene32221:CDS 40.0%
!! CTTGGTTTTCTAACAGGAAG+TGG - chr4.4:22508643-22508662 MS.gene32221:CDS 40.0%
!! TGCACTTTTGAAGATGCTTC+TGG - chr4.4:22506593-22506612 MS.gene32221:CDS 40.0%
!! TGGTATGTTTTGAATCACGC+GGG - chr4.4:22508180-22508199 MS.gene32221:CDS 40.0%
!!! TTTGGCTCGTTCTTGTTTTC+TGG - chr4.4:22505633-22505652 MS.gene32221:CDS 40.0%
AAAACTATGCTCAGGGGCTT+TGG + chr4.4:22506043-22506062 None:intergenic 45.0%
ATTCATGCAGACATGCATCC+TGG - chr4.4:22507670-22507689 MS.gene32221:intron 45.0%
CAGCAAAATCAGTCAGATGC+AGG - chr4.4:22505700-22505719 MS.gene32221:CDS 45.0%
CAGGCCTTCTACCACAATAA+AGG + chr4.4:22505794-22505813 None:intergenic 45.0%
CAGGTCCGGCATTCATAAAA+TGG - chr4.4:22505947-22505966 MS.gene32221:intron 45.0%
CATCGTACACTAGAAAGAGC+AGG - chr4.4:22505928-22505947 MS.gene32221:intron 45.0%
CCAAGACCCTAGACTTAGTA+GGG - chr4.4:22506815-22506834 MS.gene32221:intron 45.0%
CCTCGTAAAGTCTAAGTACG+AGG + chr4.4:22507216-22507235 None:intergenic 45.0%
CCTCGTACTTAGACTTTACG+AGG - chr4.4:22507213-22507232 MS.gene32221:intron 45.0%
CTAGTGTACGATGAACAACC+TGG + chr4.4:22505921-22505940 None:intergenic 45.0%
CTTTATTGTGGTAGAAGGCC+TGG - chr4.4:22505792-22505811 MS.gene32221:intron 45.0%
GAAGAATTGCCTGTTGCATC+TGG - chr4.4:22506129-22506148 MS.gene32221:intron 45.0%
GACCTTCACTGCAACTAACA+TGG + chr4.4:22506217-22506236 None:intergenic 45.0%
GATGAGCTTCAAGGACATGA+AGG - chr4.4:22506537-22506556 MS.gene32221:CDS 45.0%
GCAATACTTCCAGATGCAAC+AGG + chr4.4:22506141-22506160 None:intergenic 45.0%
GCTAAAGCATGTGAAAGGTG+AGG + chr4.4:22508347-22508366 None:intergenic 45.0%
GGATGCACAAGTGGATAAAC+AGG + chr4.4:22506465-22506484 None:intergenic 45.0%
GGTCCGGCATTCATAAAATG+GGG - chr4.4:22505949-22505968 MS.gene32221:intron 45.0%
GTCTCCATCACATTATACCC+GGG + chr4.4:22509040-22509059 None:intergenic 45.0%
GTGTGGGAGAATCTATCAGA+AGG - chr4.4:22506247-22506266 MS.gene32221:intron 45.0%
TAAAGACGCACGATGCACTT+GGG + chr4.4:22506163-22506182 None:intergenic 45.0%
TACACTAGAAAGAGCAGGTC+CGG - chr4.4:22505933-22505952 MS.gene32221:intron 45.0%
TCCAACACGTGTATTGGGAT+GGG - chr4.4:22508259-22508278 MS.gene32221:CDS 45.0%
TCGTCTTCTTGACCATCTAC+TGG - chr4.4:22508160-22508179 MS.gene32221:CDS 45.0%
TGATCCCGGGTATAATGTGA+TGG - chr4.4:22509033-22509052 MS.gene32221:CDS 45.0%
TGTAGTTCAATCATGTGGCG+AGG - chr4.4:22505433-22505452 MS.gene32221:intron 45.0%
TGTCAGCAGAAATGCAGGTT+TGG - chr4.4:22505345-22505364 MS.gene32221:CDS 45.0%
TGTTAGTTGCAGTGAAGGTC+AGG - chr4.4:22506217-22506236 MS.gene32221:intron 45.0%
TTAAAGACGCACGATGCACT+TGG + chr4.4:22506164-22506183 None:intergenic 45.0%
TTGCGTCCAACACGTGTATT+GGG - chr4.4:22508254-22508273 MS.gene32221:CDS 45.0%
TTTCCTCGATGTAGGCATGA+CGG - chr4.4:22507762-22507781 MS.gene32221:intron 45.0%
! AAAGGGCCCAGCAAACTTTT+TGG + chr4.4:22507571-22507590 None:intergenic 45.0%
! AAGAAGACGCGTCAGTGTTT+TGG + chr4.4:22507634-22507653 None:intergenic 45.0%
! AGCCACATTTTCCTGAACTC+GGG + chr4.4:22505898-22505917 None:intergenic 45.0%
! ATTGCTGCTTAGAGCTGACT+GGG - chr4.4:22509069-22509088 MS.gene32221:CDS 45.0%
! GAGCCACATTTTCCTGAACT+CGG + chr4.4:22505899-22505918 None:intergenic 45.0%
! TCGCTTTGAACTCCTTGGTT+AGG - chr4.4:22508589-22508608 MS.gene32221:CDS 45.0%
! TTCACTCGCTTTGAACTCCT+TGG - chr4.4:22508584-22508603 MS.gene32221:CDS 45.0%
! TTGATGGTAGACCCTGCATA+CGG - chr4.4:22508469-22508488 MS.gene32221:CDS 45.0%
!! AATGGTGCCATCAGTATGCT+TGG + chr4.4:22505859-22505878 None:intergenic 45.0%
!! GCCCATATTTTAAGCCCCAA+AGG - chr4.4:22507425-22507444 MS.gene32221:intron 45.0%
!! GCCCTTTGGGGCTTAAAATA+TGG + chr4.4:22507430-22507449 None:intergenic 45.0%
!! GTCCTGCATCGGTTGATATT+GGG + chr4.4:22505382-22505401 None:intergenic 45.0%
!! GTCTCAAGTTGACCTTGCAA+GGG - chr4.4:22506374-22506393 MS.gene32221:intron 45.0%
!! TAGAAGGCCTGGTCTTGTTA+GGG - chr4.4:22505803-22505822 MS.gene32221:intron 45.0%
!! TGTCCTGCATCGGTTGATAT+TGG + chr4.4:22505383-22505402 None:intergenic 45.0%
!! TGTCTCAAGTTGACCTTGCA+AGG - chr4.4:22506373-22506392 MS.gene32221:intron 45.0%
!! TGTGTGGGTATTGGGTAATC+AGG + chr4.4:22508369-22508388 None:intergenic 45.0%
!!! ATTATTTTGGCCCCGAGTTC+AGG - chr4.4:22505884-22505903 MS.gene32221:intron 45.0%
AAAACTGCGGGATGCACAAG+TGG + chr4.4:22506474-22506493 None:intergenic 50.0%
AACAAGAACGAGCCAAAGCC+AGG + chr4.4:22505630-22505649 None:intergenic 50.0%
AAGACACGTCCCTCCATTGA+CGG + chr4.4:22506434-22506453 None:intergenic 50.0%
AATGAGCAGCTTCCCTTGCA+AGG + chr4.4:22506389-22506408 None:intergenic 50.0%
ACACTGACGCGTCTTCTTTC+AGG - chr4.4:22507636-22507655 MS.gene32221:intron 50.0%
ACCTTCTCGAGTTCCCCAAA+AGG + chr4.4:22508681-22508700 None:intergenic 50.0%
AGAGTGGGTGCAATAGGTGA+GGG + chr4.4:22508207-22508226 None:intergenic 50.0%
CACCCAATATCAACCGATGC+AGG - chr4.4:22505377-22505396 MS.gene32221:CDS 50.0%
CATAAAATGGGGTCAGTGGG+CGG - chr4.4:22505960-22505979 MS.gene32221:intron 50.0%
CTGATAGATTCTCCCACACC+AGG + chr4.4:22506246-22506265 None:intergenic 50.0%
CTGTGTGTCAGCAGAAATGC+AGG - chr4.4:22505340-22505359 MS.gene32221:CDS 50.0%
GCCAAGACCCTAGACTTAGT+AGG - chr4.4:22506814-22506833 MS.gene32221:intron 50.0%
GCCCATGTTAGTTGCAGTGA+AGG - chr4.4:22506212-22506231 MS.gene32221:intron 50.0%
GTCCAACACGTGTATTGGGA+TGG - chr4.4:22508258-22508277 MS.gene32221:CDS 50.0%
GTTCAGGAAAATGTGGCTCC+AGG - chr4.4:22505900-22505919 MS.gene32221:intron 50.0%
GTTGCGTCCAACACGTGTAT+TGG - chr4.4:22508253-22508272 MS.gene32221:CDS 50.0%
TACTGTCATGGTGACCACCT+TGG - chr4.4:22508784-22508803 MS.gene32221:CDS 50.0%
TAGAGTGGGTGCAATAGGTG+AGG + chr4.4:22508208-22508227 None:intergenic 50.0%
TAGCTCTCCCTAACAAGACC+AGG + chr4.4:22505813-22505832 None:intergenic 50.0%
TCCCATCCCAATACACGTGT+TGG + chr4.4:22508263-22508282 None:intergenic 50.0%
TGCATGTGGAGAGCTAGCAT+TGG - chr4.4:22505675-22505694 MS.gene32221:CDS 50.0%
! ACTTGTGCATCCCGCAGTTT+TGG - chr4.4:22506473-22506492 MS.gene32221:intron 50.0%
! CATTGCTGCTTAGAGCTGAC+TGG - chr4.4:22509068-22509087 MS.gene32221:CDS 50.0%
! CCCTACTAAGTCTAGGGTCT+TGG + chr4.4:22506818-22506837 None:intergenic 50.0%
! CTGAACTCTCTGGTAGCGAT+CGG - chr4.4:22507785-22507804 MS.gene32221:intron 50.0%
! GCCACATTTTCCTGAACTCG+GGG + chr4.4:22505897-22505916 None:intergenic 50.0%
! TGAACTCTCTGGTAGCGATC+GGG - chr4.4:22507786-22507805 MS.gene32221:intron 50.0%
!! ACCTTTTGGGGAACTCGAGA+AGG - chr4.4:22508677-22508696 MS.gene32221:CDS 50.0%
!! GTAGAAGGCCTGGTCTTGTT+AGG - chr4.4:22505802-22505821 MS.gene32221:intron 50.0%
!!! AGTTTGCTGGGCCCTTTTGA+TGG - chr4.4:22507574-22507593 MS.gene32221:intron 50.0%
!!! GGTGACCACCTTGGTTTTAG+AGG - chr4.4:22508793-22508812 MS.gene32221:CDS 50.0%
AAAGCTGTTGCCCGCAGAGA+TGG - chr4.4:22507829-22507848 MS.gene32221:CDS 55.0%
AAGGTCAGGCATCCTGGTGT+GGG - chr4.4:22506231-22506250 MS.gene32221:intron 55.0%
AATTGCTCCATGCACTCGGC+TGG + chr4.4:22508716-22508735 None:intergenic 55.0%
ACAGAACTGCCCTGATCCGA+AGG - chr4.4:22507891-22507910 MS.gene32221:CDS 55.0%
ACGCTACTGGGCGATCTTGA+TGG - chr4.4:22508453-22508472 MS.gene32221:CDS 55.0%
ATTGCTCCATGCACTCGGCT+GGG + chr4.4:22508715-22508734 None:intergenic 55.0%
CCCAAAGGGCTCTTGCATGA+GGG - chr4.4:22507440-22507459 MS.gene32221:intron 55.0%
CCCTCATGCAAGAGCCCTTT+GGG + chr4.4:22507443-22507462 None:intergenic 55.0%
CCTCATGCAAGAGCCCTTTG+GGG + chr4.4:22507442-22507461 None:intergenic 55.0%
CTGACGCGTCTTCTTTCAGG+TGG - chr4.4:22507639-22507658 MS.gene32221:intron 55.0%
GATTGCTCAAGCAGTGAGCC+TGG - chr4.4:22505609-22505628 MS.gene32221:CDS 55.0%
GCCCCGAGTTCAGGAAAATG+TGG - chr4.4:22505893-22505912 MS.gene32221:intron 55.0%
GGAACAGTGGGTCCTTGACA+TGG - chr4.4:22507296-22507315 MS.gene32221:intron 55.0%
TCAAGCAGTGAGCCTGGCTT+TGG - chr4.4:22505615-22505634 MS.gene32221:CDS 55.0%
TCCCTCATGCAAGAGCCCTT+TGG + chr4.4:22507444-22507463 None:intergenic 55.0%
TGTTCGAGTGCCTCAGAGCA+AGG - chr4.4:22507702-22507721 MS.gene32221:intron 55.0%
! ATGGGCTTTGCTTGCTGACC+AGG + chr4.4:22506198-22506217 None:intergenic 55.0%
! TGGGCTTTGCTTGCTGACCA+GGG + chr4.4:22506197-22506216 None:intergenic 55.0%
!! TGTGCATCCCGCAGTTTTGG+TGG - chr4.4:22506476-22506495 MS.gene32221:intron 55.0%
CAGCCGTCATGCCTACATCG+AGG + chr4.4:22507768-22507787 None:intergenic 60.0%
CCCCAAAGGGCTCTTGCATG+AGG - chr4.4:22507439-22507458 MS.gene32221:intron 60.0%
CCTGAGTGTGCACTCAGCAG+CGG + chr4.4:22507860-22507879 None:intergenic 60.0%
CTGTTGCCCGCAGAGATGGT+CGG - chr4.4:22507833-22507852 MS.gene32221:CDS 60.0%
GAAGGTCAGGCATCCTGGTG+TGG - chr4.4:22506230-22506249 MS.gene32221:intron 60.0%
GCAGTGAAGGTCAGGCATCC+TGG - chr4.4:22506225-22506244 MS.gene32221:intron 60.0%
GGCATGACGGCTGAACTCTC+TGG - chr4.4:22507775-22507794 MS.gene32221:intron 60.0%
GGGAACTCGAGAAGGTGACG+CGG - chr4.4:22508685-22508704 MS.gene32221:CDS 60.0%
GGTTTGCGAGCCTTGCTCTG+AGG + chr4.4:22507715-22507734 None:intergenic 60.0%
TGGCAGCGCAAGCTTGATCC+CGG - chr4.4:22509019-22509038 MS.gene32221:CDS 60.0%
AGCGGTCCGACCATCTCTGC+GGG + chr4.4:22507842-22507861 None:intergenic 65.0%
CAGCGGTCCGACCATCTCTG+CGG + chr4.4:22507843-22507862 None:intergenic 65.0%
CCGCTGCTGAGTGCACACTC+AGG - chr4.4:22507857-22507876 MS.gene32221:CDS 65.0%
GGCAGCGCAAGCTTGATCCC+GGG - chr4.4:22509020-22509039 MS.gene32221:CDS 65.0%
GGTTCACCCAGCCGAGTGCA+TGG - chr4.4:22508706-22508725 MS.gene32221:CDS 65.0%
GGGGTCAGTGGGCGGCTACA+CGG - chr4.4:22505968-22505987 MS.gene32221:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 22505280 22509135 22505280 ID=MS.gene32221
chr4.4 mRNA 22505280 22509135 22505280 ID=MS.gene32221.t1;Parent=MS.gene32221
chr4.4 exon 22507801 22509135 22507801 ID=MS.gene32221.t1.exon1;Parent=MS.gene32221.t1
chr4.4 CDS 22507801 22509135 22507801 ID=cds.MS.gene32221.t1;Parent=MS.gene32221.t1
chr4.4 exon 22506491 22506757 22506491 ID=MS.gene32221.t1.exon2;Parent=MS.gene32221.t1
chr4.4 CDS 22506491 22506757 22506491 ID=cds.MS.gene32221.t1;Parent=MS.gene32221.t1
chr4.4 exon 22505601 22505756 22505601 ID=MS.gene32221.t1.exon3;Parent=MS.gene32221.t1
chr4.4 CDS 22505601 22505756 22505601 ID=cds.MS.gene32221.t1;Parent=MS.gene32221.t1
chr4.4 exon 22505280 22505399 22505280 ID=MS.gene32221.t1.exon4;Parent=MS.gene32221.t1
chr4.4 CDS 22505280 22505399 22505280 ID=cds.MS.gene32221.t1;Parent=MS.gene32221.t1
Gene Sequence

>MS.gene32221

ATGCTTGGACACATTGGGAGGGCTGCCGAATCTATTCTTAGAATCCATAAAAGCAGATATCTGTGTGTCAGCAGAAATGCAGGTTTGGTCTCAGTTGCACCCAATATCAACCGATGCAGGACATATATGCAGTATAAGTTTCCCAGTGAAGGATGTAGTTCAATCATGTGGCGAGGAGCTAGAGAAAACTTTCACAAAGGCCGTTCTTTTTGGAACTTCACTTTATTCTCAGCAAAAAATATTACAGCACATAAATCTCAAATTGCTTGGAAATTGCTGCATAAAATATATTCTTCCAATGGTTATAGTGGATCTACCTCTGTACATATGATTGCTCAAGCAGTGAGCCTGGCTTTGGCTCGTTCTTGTTTTCTGGTTCCTAGTATTTTTGCGTTTGCATGTGGAGAGCTAGCATTGGCTCAGCAAAATCAGTCAGATGCAGGGAGTTACCCTTCACCAAATGCTTTGTGTATGAGTGCACAAGATGGATACAGTTACATGTTTGCATTTGCCTTTATTGTGGTAGAAGGCCTGGTCTTGTTAGGGAGAGCTATATATCTAGCAATATTATTCTCTCCAAGCATACTGATGGCACCATTTGCAGATTATTTTGGCCCCGAGTTCAGGAAAATGTGGCTCCAGGTTGTTCATCGTACACTAGAAAGAGCAGGTCCGGCATTCATAAAATGGGGTCAGTGGGCGGCTACACGGCCTGATCTATTTCCTCAAGATATATGTACTAAGCTTGCAGAACTTCATTCCAAAGCCCCTGAGCATAGTTTTAGCTACACAAAGAAAACTATAGAAAGCGCATTTGGTCGTAAAATTTCTGAAATTTTTGAGAATTTTGAAGAATTGCCTGTTGCATCTGGAAGTATTGCCCAAGTGCATCGTGCGTCTTTAAAATATCGTTACCCTGGTCAGCAAGCAAAGCCCATGTTAGTTGCAGTGAAGGTCAGGCATCCTGGTGTGGGAGAATCTATCAGAAGGGATTTCGCTATTATCAATTTTGTGGCAAAATCTTCAAAGTTCATCCCTGCACTTAATTGGTTAAGGTTGGACGAAAGTGTTCAGCAGTTTGCAGTTTTCATGATGTCTCAAGTTGACCTTGCAAGGGAAGCTGCTCATTTGAACCGCTTTATTTACAATTTCCGTCAATGGAGGGACGTGTCTTTCCCTAAGCCTGTTTATCCACTTGTGCATCCCGCAGTTTTGGTGGAAACATATGAAAATGGAGAAAGTGTCTCGCATTATGTTGATGAGCTTCAAGGACATGAAGGGATCAAAAGCGCTCTTGCTCATATTGGTAGTAATGCACTTTTGAAGATGCTTCTGGTAAGATATCATCTATTTACATTTTTCCTTTCTTGTTATATATATATTGTGGTTGGTGACAGAGCTGCTATTAAATACTTCTTTTTATTAGCATTCTTTGTGTGTTGTGTCATTAAGGAAAATTCATGATAGCTAATGTGCCCTGAAATGAAGTTAATATGGAGTAGAGAGATATATAGCTATTGTTAGTGGTGGCAGAGCCAAGACCCTAGACTTAGTAGGGACAAGTATTCTTTTTTTTCCTACTTAACCAAGTTTGTTTGATCAACAAATTTACCCCTGAAATTTGAAGTGACGAAAAATTAAGTACTTATTTGTAATGTCTTGTTAGTCTGGTACAAAAAACAAAGTGGACCACAGTTTTTATTACAATACTTATAGTTTATATTTGTCCTGTACATTTTTCTACACTATGAACCCACTGGATAATCAACTTCTATTCCATTAAATATGTATCTCTTTTTAATTTACATTGTTAGTCAAAGGTTATTTGACTTGTGTGTTATCTTATCTTGTGTTTGTTTTTAATAAAAGAAAATTACCCTGTTAATATTTTTGCCAACAATTATCAACTATGCTCTGTAGTTTATAACGCCTTCCTCGTACTTAGACTTTACGAGGATACCTTGTCATCTATTATAGTACCTGTTAGATACAGCTCACCTAATAACTTTAGTTTTTGGAACAGTGGGTCCTTGACATGGTATTAGAACCTTTATGACCAAGTGGTCAAGAATTCAATCCTTATGAAATTCAATCTTGTAAATAAAAAATTAAATTTCAGCATAAGGTGAGATGGGTATATTCACTGCCCATATTTTAAGCCCCAAAGGGCTCTTGCATGAGGGAGTGTGCTAGATATAAACTATTCGCAAACATTCAAGCAACTTAAGTAAGTAGGATTGGTTCTTGACAGTACTCATTTCTCTTTTATTTCATAAATTTTCTTTCATCCAAAAAGTTTGCTGGGCCCTTTTGATGGCTTTAAACTTGAATGCCATACAATTGTTTAGCTTCCAAAACACTGACGCGTCTTCTTTCAGGTGGACAACTTCATTCATGCAGACATGCATCCTGGAAATATTCTTGTTCGAGTGCCTCAGAGCAAGGCTCGCAAACCGTTCTTCAAATCAAAGCCTCATGTAATTTTCCTCGATGTAGGCATGACGGCTGAACTCTCTGGTAGCGATCGGGTAAATTTAGTGGAGTTTTTCAAAGCTGTTGCCCGCAGAGATGGTCGGACCGCTGCTGAGTGCACACTCAGGTTGTCAAAGCAACAGAACTGCCCTGATCCGAAGGCTTATATTGAGGTAATTTAGTTATTATGTATATTAGCTTATGGTTGGATGAGTTTTACATGAAGGTACAAGTTTTATTTTATCACTTGCATGCGCGCACGCACACACACACACACACACACACTCACACATATATATATGTACACTGAAAGTGTATCGTGTATTTATGCGAGAGCATACTCGTGTGATAATTTTACAATCATAACCGATGATCAGGATTAAATTTAAGGAAAATAATCACGAGTTCTCGTCTTCTTGACCATCTACTGGTATGTTTTGAATCACGCGGGACCCTCACCTATTGCACCCACTCTAAATCTTATATTTGTAGTTTAATAAAGTTGCGTCCAACACGTGTATTGGGATGGGAGAATTTGTGCTGCACCACATATCTAAACGGATACTATTTGTGATTGTATTGTCTATTAATTCCTCACCTTTCACATGCTTTAGCCTGATTACCCAATACCCACACACAAACACAATAGAATTTTTGGTATCTACTTTCAATGTTTATGTGCATACCAATACAATTAATATACGCTACTGGGCGATCTTGATGGTAGACCCTGCATACGGATCTATTTCACTTTGGTAAATTTTTGCCTAGCCATTGTCATTGGCTTTTTATAAACTGCTTCATCTACAAATGTTGTTTTTTCTTATTTAAGTTCACTCGCTTTGAACTCCTTGGTTAGGATTGTGAGTCATTTTATACATTTGGTTTTCTCTTGGTTTTCTAACAGGAAGTGGAAAAGGCATTTACCTTTTGGGGAACTCGAGAAGGTGACGCGGTTCACCCAGCCGAGTGCATGGAGCAATTACTTGAGAAAGTTAGGCGCCATAGAGTTAATATTGATGGCAATGTTTGTACTGTCATGGTGACCACCTTGGTTTTAGAGGTAATTCTTTGTTACATTACCACTGTAACAGAGTTTATGTTTATTTTGTGATTGACATGCATGGAAAATAATGGTGAAAGGCCGAAAGCATAAATTAGATTTTAGAATGTTTTCTTTTCGCATGCACCAAACCATCTACATTGGTAATTATTAAAAGCTGGTGTGACTGACTTTATACATTGGTGAATATATAATTTGTAGGGTTGGCAGCGCAAGCTTGATCCCGGGTATAATGTGATGGAGACACTGCAGACATTGCTGCTTAGAGCTGACTGGGCAAAGTCTTTTGCTTACACAATTCAGAGTCTGATGGCCCCTTGA
Protein sequence

>MS.gene32221.t1

MLGHIGRAAESILRIHKSRYLCVSRNAGLVSVAPNINRCRTYMQYKFPSEGCSSIMWRGARENFHKGRSFWNFTLFSAKNITAHKSQIAWKLLHKIYSSNGYSGSTSVHMIAQAVSLALARSCFLVPSIFAFACGELALAQQNQSDAGSYPSPNALCMSAQDGYSYMFAFAFIVVEGLVLLGRAIYLAILFSPSILMAPFADYFGPEFRKMWLQVVHRTLERAGPAFIKWGQWAATRPDLFPQDICTKLAELHSKAPEHSFSYTKKTIESAFGRKISEIFENFEELPVASGSIAQVHRASLKYRYPGQQAKPMLVAVKVRHPGVGESIRRDFAIINFVAKSSKFIPALNWLRLDESVQQFAVFMMSQVDLAREAAHLNRFIYNFRQWRDVSFPKPVYPLVHPAVLVETYENGESVSHYVDELQGHEGIKSALAHIGSNALLKMLLVDNFIHADMHPGNILVRVPQSKARKPFFKSKPHVIFLDVGMTAELSGSDRVNLVEFFKAVARRDGRTAAECTLRLSKQQNCPDPKAYIEEVEKAFTFWGTREGDAVHPAECMEQLLEKVRRHRVNIDGNVCTVMVTTLVLEGWQRKLDPGYNVMETLQTLLLRADWAKSFAYTIQSLMAP