AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32296

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32296.t1 MTR_8g461160 91.899 395 29 2 1 393 1 394 0.0 716
MS.gene32296.t1 MTR_3g101180 70.250 400 112 3 1 393 1 400 0.0 561
MS.gene32296.t1 MTR_3g101010 67.876 386 121 2 6 391 5 387 0.0 520
MS.gene32296.t1 MTR_7g451890 66.327 392 131 1 2 392 10 401 0.0 511
MS.gene32296.t1 MTR_7g047780 65.561 392 134 1 2 392 10 401 0.0 509
MS.gene32296.t1 MTR_7g050750 65.561 392 134 1 2 392 4 395 4.26e-180 506
MS.gene32296.t1 MTR_4g045707 64.051 395 127 3 1 390 1 385 3.63e-179 504
MS.gene32296.t1 MTR_3g018740 65.810 389 127 3 6 393 5 388 1.75e-178 502
MS.gene32296.t1 MTR_7g050810 63.706 394 138 2 2 392 12 403 3.66e-174 491
MS.gene32296.t1 MTR_3g101130 61.125 391 148 1 1 391 1 387 1.02e-172 487
MS.gene32296.t1 MTR_3g101030 61.461 397 132 5 12 391 35 427 3.45e-169 480
MS.gene32296.t1 MTR_8g027395 62.176 386 139 2 6 391 17 395 4.32e-169 478
MS.gene32296.t1 MTR_3g048000 60.925 389 128 4 4 391 25 390 3.32e-162 461
MS.gene32296.t1 MTR_8g027420 59.335 391 141 3 1 391 1 373 2.25e-158 450
MS.gene32296.t1 MTR_4g088525 60.221 362 139 1 30 391 11 367 6.34e-157 446
MS.gene32296.t1 MTR_2g046030 58.524 393 149 5 6 393 5 388 2.12e-153 438
MS.gene32296.t1 MTR_3g015620 59.391 394 123 6 6 392 16 379 2.15e-153 438
MS.gene32296.t1 MTR_2g045980 58.524 393 149 5 6 393 5 388 4.26e-152 435
MS.gene32296.t1 MTR_3g101050 59.079 391 140 5 4 393 27 398 6.09e-151 432
MS.gene32296.t1 MTR_4g088535 56.883 385 143 4 4 388 5 366 6.19e-147 422
MS.gene32296.t1 MTR_3g101200 54.822 394 150 4 1 393 26 392 4.74e-138 399
MS.gene32296.t1 MTR_3g101190 55.220 364 95 3 30 391 13 310 7.32e-130 375
MS.gene32296.t1 MTR_3g102920 52.488 402 135 7 4 391 49 408 1.28e-129 379
MS.gene32296.t1 MTR_7g050830 56.426 319 108 2 2 300 12 319 8.78e-122 355
MS.gene32296.t1 MTR_3g100995 64.591 257 87 2 136 392 11 263 6.29e-116 338
MS.gene32296.t1 MTR_3g015760 66.792 265 77 5 132 392 23 280 1.89e-110 325
MS.gene32296.t1 MTR_3g018747 61.887 265 80 5 132 393 9 255 1.45e-106 314
MS.gene32296.t1 MTR_3g100010 51.935 310 101 3 33 309 42 336 1.47e-102 307
MS.gene32296.t1 MTR_3g100520 60.181 221 72 4 173 391 1 207 1.43e-85 259
MS.gene32296.t1 MTR_3g111160 59.276 221 85 3 173 391 1 218 5.68e-82 250
MS.gene32296.t1 MTR_3g114310 40.102 394 210 11 12 390 25 407 2.76e-80 253
MS.gene32296.t1 MTR_3g101110 56.846 241 71 5 132 367 68 280 5.57e-79 245
MS.gene32296.t1 MTR_3g099970 61.735 196 58 2 132 327 20 198 4.33e-78 239
MS.gene32296.t1 MTR_2g046410 58.571 210 78 3 189 393 144 349 8.89e-77 241
MS.gene32296.t1 MTR_3g101150 68.790 157 46 2 238 391 1 157 8.35e-69 214
MS.gene32296.t1 MTR_3g101020 55.615 187 55 2 206 392 1 159 2.86e-60 192
MS.gene32296.t1 MTR_3g111150 53.073 179 58 2 8 186 1 153 2.50e-56 182
MS.gene32296.t1 MTR_3g102870 59.355 155 63 0 197 351 3 157 4.91e-56 182
MS.gene32296.t1 MTR_3g100990 56.849 146 61 1 6 151 5 148 1.09e-43 159
MS.gene32296.t1 MTR_4g093550 61.290 93 32 2 5 97 5 93 2.16e-25 99.0
MS.gene32296.t1 MTR_6g046890 59.155 71 26 1 29 99 20 87 1.42e-18 82.0
MS.gene32296.t1 MTR_3g101140 59.701 67 27 0 325 391 48 114 2.90e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32296.t1 AT1G47710 50.515 388 188 4 6 391 5 390 3.18e-135 392
MS.gene32296.t1 AT1G47710 50.127 393 182 5 6 391 32 417 1.58e-134 391
MS.gene32296.t1 AT3G45220 44.162 394 203 6 6 390 5 390 8.94e-116 343
MS.gene32296.t1 AT2G25240 45.501 389 197 8 6 390 5 382 1.70e-113 337
MS.gene32296.t1 AT2G26390 44.444 396 195 8 6 390 5 386 6.14e-109 325
MS.gene32296.t1 AT1G64030 42.931 389 196 5 6 390 5 371 3.00e-100 303
MS.gene32296.t1 AT1G62170 41.823 373 177 5 29 390 88 431 6.17e-98 298
MS.gene32296.t1 AT2G14540 42.010 388 199 5 6 390 33 397 2.42e-96 293
MS.gene32296.t1 AT2G35580 42.308 364 176 9 36 390 33 371 3.57e-93 284
MS.gene32296.t1 AT1G62170 38.424 406 177 6 29 390 88 464 1.11e-90 281
MS.gene32296.t1 AT1G64010 44.091 220 96 5 173 390 1 195 8.29e-58 187
MS.gene32296.t1 AT1G64010 43.415 205 89 5 188 390 2 181 7.45e-51 169
MS.gene32296.t1 AT1G62160 40.513 195 89 6 198 390 49 218 5.46e-37 134
MS.gene32296.t1 AT1G63280 55.932 118 48 2 129 244 5 120 6.70e-37 130
MS.gene32296.t1 AT1G51330 49.107 112 54 1 130 238 29 140 3.32e-26 104
MS.gene32296.t1 AT1G64020 32.143 112 73 3 13 123 12 121 1.35e-12 64.7

Find 79 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATGCACTATACTTTAA+AGG 0.146562 8.3:+49168329 MS.gene32296:CDS
CCGAAATTCAAGATTTCTTT+TGG 0.171353 8.3:+49168659 MS.gene32296:CDS
TCTGGAACCATACTCTTTAT+TGG 0.177113 8.3:+49168953 MS.gene32296:CDS
TAGTGGAAGTTGGTGCTTTC+AGG 0.177468 8.3:+49168633 MS.gene32296:CDS
CCTTCGTTGGCTTTCCTTAA+TGG 0.196609 8.3:+49167858 MS.gene32296:CDS
TGACAAAAGAAGCAAATTTA+TGG 0.225380 8.3:+49168228 MS.gene32296:CDS
AGCTTCCGAATCTAAGTTCT+TGG 0.253184 8.3:+49168589 MS.gene32296:CDS
TTGTCTTCACCTTGCGTATA+AGG 0.282322 8.3:-49168497 None:intergenic
AGCCTATGGGGAAAGCTATA+TGG 0.293869 8.3:+49168838 MS.gene32296:CDS
CTGGAACCATACTCTTTATT+GGG 0.301041 8.3:+49168954 MS.gene32296:CDS
GGTTTCTTTGTATTCTTGTT+TGG 0.323549 8.3:-49167655 None:intergenic
GAAATATGTCGGACACATAA+AGG 0.333781 8.3:-49168778 None:intergenic
CCAAAAGAAATCTTGAATTT+CGG 0.337465 8.3:-49168659 None:intergenic
CAGTATATCAGTGTTCTTGA+TGG 0.364509 8.3:+49168455 MS.gene32296:CDS
GTTCAACCCACATACCATTA+AGG 0.368568 8.3:-49167872 None:intergenic
CAAGCTTCCTCGCGAATTAG+TGG 0.375298 8.3:+49168616 MS.gene32296:CDS
CACTTGCCCAATAAAGAGTA+TGG 0.383332 8.3:-49168960 None:intergenic
AGCACGATGTGGAGCGATAA+TGG 0.391927 8.3:-49167693 None:intergenic
GCCTATGGGGAAAGCTATAT+GGG 0.398940 8.3:+49168839 MS.gene32296:CDS
TGGCTTTCCTTAATGGTATG+TGG 0.399840 8.3:+49167865 MS.gene32296:CDS
CTCTCCAATGCTGCTCCTGC+CGG 0.400280 8.3:+49167831 MS.gene32296:CDS
GTCAAAGCTCCTTCGGAGAA+AGG 0.403461 8.3:-49168725 None:intergenic
TTTCTTCCTGATGCAAAAGA+TGG 0.410392 8.3:+49168542 MS.gene32296:CDS
TTGTTTGGAGAACAAATGTT+TGG 0.415802 8.3:-49167640 None:intergenic
GACAAAAGAAGCAAATTTAT+GGG 0.422156 8.3:+49168229 MS.gene32296:CDS
TTGATCAGAGAAGATTTGTC+TGG 0.431879 8.3:+49168935 MS.gene32296:CDS
TCCTCCGGCAGGAGCAGCAT+TGG 0.439334 8.3:-49167835 None:intergenic
ATAGCTTTCCCCATAGGCTT+CGG 0.456657 8.3:-49168834 None:intergenic
GTGCTGAGCATCATCGCTGC+TGG 0.468288 8.3:+49167711 MS.gene32296:CDS
TTTATTGAAGTAAACGAAGA+AGG 0.470006 8.3:+49168809 MS.gene32296:CDS
TGATCAACTACGAAGTTTAT+CGG 0.477413 8.3:-49168899 None:intergenic
CCCCATATAGCTTTCCCCAT+AGG 0.478570 8.3:-49168840 None:intergenic
GTCATGAAGGGAACCTTGAT+CGG 0.480422 8.3:-49168419 None:intergenic
GTGTTCCAAGAACTTAGATT+CGG 0.487041 8.3:-49168594 None:intergenic
GAAGAAGGTACCGAAGCCTA+TGG 0.487750 8.3:+49168824 MS.gene32296:CDS
CAAAGAAACCTTGGTTAGGT+TGG 0.494142 8.3:-49167610 None:intergenic
ATCATCGCTGCTGGCTCTGA+AGG 0.496680 8.3:+49167720 MS.gene32296:CDS
TTTCCGTGGATTTGGATTGA+AGG 0.501160 8.3:-49167770 None:intergenic
TGGTCAAAGAAACCTTGGTT+AGG 0.502434 8.3:-49167614 None:intergenic
AGGAGCAGCATTGGAGAGCA+CGG 0.507039 8.3:-49167826 None:intergenic
GGCTTTCCTTAATGGTATGT+GGG 0.508630 8.3:+49167866 MS.gene32296:CDS
TCCTGCCGGAGGACCTTCGT+TGG 0.508638 8.3:+49167845 MS.gene32296:CDS
GATATACTGCATCTCATAGC+TGG 0.512249 8.3:-49168441 None:intergenic
TCTCAATGGTAGCCCGATCA+AGG 0.512612 8.3:+49168406 MS.gene32296:CDS
AAGAAGGTACCGAAGCCTAT+GGG 0.513028 8.3:+49168825 MS.gene32296:CDS
GAAAGCCAACGAAGGTCCTC+CGG 0.516566 8.3:-49167850 None:intergenic
GCCAACGAAGGTCCTCCGGC+AGG 0.525135 8.3:-49167846 None:intergenic
CATCTCATAGCTGGTCATGA+AGG 0.531770 8.3:-49168432 None:intergenic
GGCGATGGTCAAAGAAACCT+TGG 0.531986 8.3:-49167619 None:intergenic
TGCATTTGCAAAGATGAGGT+TGG 0.533879 8.3:-49168315 None:intergenic
AAAAGACTTGTGAAATATGT+CGG 0.536698 8.3:-49168789 None:intergenic
CTTTAAAGGAGTGTGGAAAG+AGG 0.539924 8.3:+49168343 MS.gene32296:CDS
GTTAAACTATTAAATGACTC+AGG 0.540790 8.3:-49168293 None:intergenic
TCCAATGCTGCTCCTGCCGG+AGG 0.541408 8.3:+49167834 MS.gene32296:CDS
TCATGAAGGGAACCTTGATC+GGG 0.545518 8.3:-49168418 None:intergenic
ATTCCTTCAATCCAAATCCA+CGG 0.547231 8.3:+49167767 MS.gene32296:CDS
TTTATCGGTGGCGGAGTACG+AGG 0.556228 8.3:-49168884 None:intergenic
TAATGGTGAAAACACAACAT+TGG 0.559392 8.3:-49167676 None:intergenic
CTTCGTCTTCCTTATACGCA+AGG 0.561814 8.3:+49168488 MS.gene32296:CDS
TTCAGTTGATTTCGTTAACA+AGG 0.566863 8.3:+49167959 MS.gene32296:CDS
CACTATACTTTAAAGGAGTG+TGG 0.566946 8.3:+49168336 MS.gene32296:CDS
ACTGATATGCTAAAAGAGCT+AGG 0.568471 8.3:+49168692 MS.gene32296:CDS
CCTCGCGAATTAGTGGAAGT+TGG 0.573383 8.3:+49168623 MS.gene32296:CDS
GGAGAACAAATGTTTGGCGA+TGG 0.575181 8.3:-49167634 None:intergenic
ATCTCATAGCTGGTCATGAA+GGG 0.593119 8.3:-49168431 None:intergenic
AGTTAAGATCACTTGTCAAG+AGG 0.597230 8.3:-49168989 None:intergenic
CGATGATGCTCAGCACGATG+TGG 0.607651 8.3:-49167704 None:intergenic
ACTACGAAGTTTATCGGTGG+CGG 0.608711 8.3:-49168893 None:intergenic
ATAGTGCATTTGCAAAGATG+AGG 0.626715 8.3:-49168319 None:intergenic
AGAAGGTACCGAAGCCTATG+GGG 0.654044 8.3:+49168826 MS.gene32296:CDS
CACGGAGGAAACAAGCTGAG+AGG 0.674325 8.3:-49167808 None:intergenic
ATCAATCACCAACCTAACCA+AGG 0.679210 8.3:+49167602 MS.gene32296:CDS
CCATTAAGGAAAGCCAACGA+AGG 0.699382 8.3:-49167858 None:intergenic
CCAACTTCCACTAATTCGCG+AGG 0.703336 8.3:-49168623 None:intergenic
CCTATGGGGAAAGCTATATG+GGG 0.715773 8.3:+49168840 MS.gene32296:CDS
AGCAGCATTGGAGAGCACGG+AGG 0.716416 8.3:-49167823 None:intergenic
CTTGCGTATAAGGAAGACGA+AGG 0.726910 8.3:-49168487 None:intergenic
TATGCTAAAAGAGCTAGGCG+TGG 0.728186 8.3:+49168697 MS.gene32296:CDS
TCAACTACGAAGTTTATCGG+TGG 0.732579 8.3:-49168896 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATCAAACATAAAATTAA+CGG - chr8.3:49168189-49168208 None:intergenic 10.0%
!! ATTATTTGATAAAAGAATTA+GGG + chr8.3:49168079-49168098 MS.gene32296:intron 10.0%
!! TATTATTTGATAAAAGAATT+AGG + chr8.3:49168078-49168097 MS.gene32296:intron 10.0%
!! CTTCAAGATAAATAAAAAAA+AGG - chr8.3:49168001-49168020 None:intergenic 15.0%
!!! TATGTTTGATATTGTTTTAA+AGG + chr8.3:49168196-49168215 MS.gene32296:intron 15.0%
!!! TTTTATGTGGTATTTTATTT+TGG + chr8.3:49168040-49168059 MS.gene32296:intron 15.0%
!!! AAAATAATAAAAGCACGATA+TGG - chr8.3:49168024-49168043 None:intergenic 20.0%
!!! AGCAATATTAGTTTTGAAAT+CGG - chr8.3:49167940-49167959 None:intergenic 20.0%
! AAAAGACTTGTGAAATATGT+CGG - chr8.3:49168792-49168811 None:intergenic 25.0%
! AAGAACAAAGATCGATATTT+AGG - chr8.3:49168115-49168134 None:intergenic 25.0%
! CCAAAAGAAATCTTGAATTT+CGG - chr8.3:49168662-49168681 None:intergenic 25.0%
! GACAAAAGAAGCAAATTTAT+GGG + chr8.3:49168229-49168248 MS.gene32296:CDS 25.0%
! GTTAAACTATTAAATGACTC+AGG - chr8.3:49168296-49168315 None:intergenic 25.0%
! TGACAAAAGAAGCAAATTTA+TGG + chr8.3:49168228-49168247 MS.gene32296:CDS 25.0%
! TTTATTGAAGTAAACGAAGA+AGG + chr8.3:49168809-49168828 MS.gene32296:CDS 25.0%
!! CGAAATTCAAGATTTCTTTT+GGG + chr8.3:49168660-49168679 MS.gene32296:CDS 25.0%
!! TATGATTTTCACATTCTCAA+TGG + chr8.3:49168392-49168411 MS.gene32296:CDS 25.0%
!!! CGTGCTTTTATTATTTTATG+TGG + chr8.3:49168027-49168046 MS.gene32296:intron 25.0%
CTCTGATCAAAAACAAGAAA+GGG - chr8.3:49168925-49168944 None:intergenic 30.0%
GCAAATGCACTATACTTTAA+AGG + chr8.3:49168329-49168348 MS.gene32296:CDS 30.0%
GGTTTCTTTGTATTCTTGTT+TGG - chr8.3:49167658-49167677 None:intergenic 30.0%
TAATGGTGAAAACACAACAT+TGG - chr8.3:49167679-49167698 None:intergenic 30.0%
TCTCTGATCAAAAACAAGAA+AGG - chr8.3:49168926-49168945 None:intergenic 30.0%
TGATCAACTACGAAGTTTAT+CGG - chr8.3:49168902-49168921 None:intergenic 30.0%
TTGTTTGGAGAACAAATGTT+TGG - chr8.3:49167643-49167662 None:intergenic 30.0%
! AAAGTTGTTTAGATTTTCCG+TGG - chr8.3:49167787-49167806 None:intergenic 30.0%
! CCGAAATTCAAGATTTCTTT+TGG + chr8.3:49168659-49168678 MS.gene32296:CDS 30.0%
! TTCAGTTGATTTCGTTAACA+AGG + chr8.3:49167959-49167978 MS.gene32296:CDS 30.0%
!!! GAAAGAACTTGTTTTTGTGT+AGG - chr8.3:49167747-49167766 None:intergenic 30.0%
ACTGATATGCTAAAAGAGCT+AGG + chr8.3:49168692-49168711 MS.gene32296:CDS 35.0%
ATAGTGCATTTGCAAAGATG+AGG - chr8.3:49168322-49168341 None:intergenic 35.0%
ATTCCTTCAATCCAAATCCA+CGG + chr8.3:49167767-49167786 MS.gene32296:CDS 35.0%
CAGTATATCAGTGTTCTTGA+TGG + chr8.3:49168455-49168474 MS.gene32296:CDS 35.0%
CTGGAACCATACTCTTTATT+GGG + chr8.3:49168954-49168973 MS.gene32296:CDS 35.0%
GAAATATGTCGGACACATAA+AGG - chr8.3:49168781-49168800 None:intergenic 35.0%
GTGTTCCAAGAACTTAGATT+CGG - chr8.3:49168597-49168616 None:intergenic 35.0%
TTGATCAGAGAAGATTTGTC+TGG + chr8.3:49168935-49168954 MS.gene32296:CDS 35.0%
TTTCTTCCTGATGCAAAAGA+TGG + chr8.3:49168542-49168561 MS.gene32296:CDS 35.0%
! AAAAGAATTAGGGTACCAAG+TGG + chr8.3:49168089-49168108 MS.gene32296:intron 35.0%
! AACAATCCATCTTTTGCATC+AGG - chr8.3:49168551-49168570 None:intergenic 35.0%
! AATCGGTAGCCACAATTTTT+CGG - chr8.3:49167923-49167942 None:intergenic 35.0%
! CACTATACTTTAAAGGAGTG+TGG + chr8.3:49168336-49168355 MS.gene32296:CDS 35.0%
! CATTTTTGTCAAAGCTCCTT+CGG - chr8.3:49168735-49168754 None:intergenic 35.0%
! GTTTAGATTTTCCGTGGATT+TGG - chr8.3:49167781-49167800 None:intergenic 35.0%
! TCTGGAACCATACTCTTTAT+TGG + chr8.3:49168953-49168972 MS.gene32296:CDS 35.0%
ACCTTCCTTCCGAAAAATTG+TGG + chr8.3:49167911-49167930 MS.gene32296:CDS 40.0%
AGATCGATATTTAGGCCACT+TGG - chr8.3:49168107-49168126 None:intergenic 40.0%
AGCTTCCGAATCTAAGTTCT+TGG + chr8.3:49168589-49168608 MS.gene32296:CDS 40.0%
ATCAATCACCAACCTAACCA+AGG + chr8.3:49167602-49167621 MS.gene32296:CDS 40.0%
ATCTCATAGCTGGTCATGAA+GGG - chr8.3:49168434-49168453 None:intergenic 40.0%
CAAAGAAACCTTGGTTAGGT+TGG - chr8.3:49167613-49167632 None:intergenic 40.0%
CACTTGCCCAATAAAGAGTA+TGG - chr8.3:49168963-49168982 None:intergenic 40.0%
GATATACTGCATCTCATAGC+TGG - chr8.3:49168444-49168463 None:intergenic 40.0%
GGCTTTCCTTAATGGTATGT+GGG + chr8.3:49167866-49167885 MS.gene32296:CDS 40.0%
GTTCAACCCACATACCATTA+AGG - chr8.3:49167875-49167894 None:intergenic 40.0%
TCAACTACGAAGTTTATCGG+TGG - chr8.3:49168899-49168918 None:intergenic 40.0%
TGCATTTGCAAAGATGAGGT+TGG - chr8.3:49168318-49168337 None:intergenic 40.0%
TGGCTTTCCTTAATGGTATG+TGG + chr8.3:49167865-49167884 MS.gene32296:CDS 40.0%
TGGGCTGAAAAAGAGACAAA+TGG + chr8.3:49168248-49168267 MS.gene32296:CDS 40.0%
TGGTCAAAGAAACCTTGGTT+AGG - chr8.3:49167617-49167636 None:intergenic 40.0%
TTGTCTTCACCTTGCGTATA+AGG - chr8.3:49168500-49168519 None:intergenic 40.0%
TTTCCGTGGATTTGGATTGA+AGG - chr8.3:49167773-49167792 None:intergenic 40.0%
! AGGAGCTTTGACAAAAATGG+TGG + chr8.3:49168736-49168755 MS.gene32296:CDS 40.0%
! CGAAGGAGCTTTGACAAAAA+TGG + chr8.3:49168733-49168752 MS.gene32296:CDS 40.0%
! CTTTAAAGGAGTGTGGAAAG+AGG + chr8.3:49168343-49168362 MS.gene32296:CDS 40.0%
ACTACGAAGTTTATCGGTGG+CGG - chr8.3:49168896-49168915 None:intergenic 45.0%
AGCCTATGGGGAAAGCTATA+TGG + chr8.3:49168838-49168857 MS.gene32296:CDS 45.0%
ATAGCTTTCCCCATAGGCTT+CGG - chr8.3:49168837-49168856 None:intergenic 45.0%
CATCTCATAGCTGGTCATGA+AGG - chr8.3:49168435-49168454 None:intergenic 45.0%
CCATTAAGGAAAGCCAACGA+AGG - chr8.3:49167861-49167880 None:intergenic 45.0%
CCTATGGGGAAAGCTATATG+GGG + chr8.3:49168840-49168859 MS.gene32296:CDS 45.0%
CTTCGTCTTCCTTATACGCA+AGG + chr8.3:49168488-49168507 MS.gene32296:CDS 45.0%
CTTGCGTATAAGGAAGACGA+AGG - chr8.3:49168490-49168509 None:intergenic 45.0%
GCCTATGGGGAAAGCTATAT+GGG + chr8.3:49168839-49168858 MS.gene32296:CDS 45.0%
GGAGAACAAATGTTTGGCGA+TGG - chr8.3:49167637-49167656 None:intergenic 45.0%
GTCATGAAGGGAACCTTGAT+CGG - chr8.3:49168422-49168441 None:intergenic 45.0%
TATGCTAAAAGAGCTAGGCG+TGG + chr8.3:49168697-49168716 MS.gene32296:CDS 45.0%
TCATGAAGGGAACCTTGATC+GGG - chr8.3:49168421-49168440 None:intergenic 45.0%
!! AAGAAGGTACCGAAGCCTAT+GGG + chr8.3:49168825-49168844 MS.gene32296:CDS 45.0%
!! CCTTCGTTGGCTTTCCTTAA+TGG + chr8.3:49167858-49167877 MS.gene32296:CDS 45.0%
!! GCCACAATTTTTCGGAAGGA+AGG - chr8.3:49167915-49167934 None:intergenic 45.0%
!! GGTAGCCACAATTTTTCGGA+AGG - chr8.3:49167919-49167938 None:intergenic 45.0%
!! GTGGTTTTACCTTTCTCCGA+AGG + chr8.3:49168716-49168735 MS.gene32296:CDS 45.0%
!! TAGTGGAAGTTGGTGCTTTC+AGG + chr8.3:49168633-49168652 MS.gene32296:CDS 45.0%
AGCACGATGTGGAGCGATAA+TGG - chr8.3:49167696-49167715 None:intergenic 50.0%
CAAGCTTCCTCGCGAATTAG+TGG + chr8.3:49168616-49168635 MS.gene32296:CDS 50.0%
CCAACTTCCACTAATTCGCG+AGG - chr8.3:49168626-49168645 None:intergenic 50.0%
CCCCATATAGCTTTCCCCAT+AGG - chr8.3:49168843-49168862 None:intergenic 50.0%
CCTCGCGAATTAGTGGAAGT+TGG + chr8.3:49168623-49168642 MS.gene32296:CDS 50.0%
GGCGATGGTCAAAGAAACCT+TGG - chr8.3:49167622-49167641 None:intergenic 50.0%
GTCAAAGCTCCTTCGGAGAA+AGG - chr8.3:49168728-49168747 None:intergenic 50.0%
TCTCAATGGTAGCCCGATCA+AGG + chr8.3:49168406-49168425 MS.gene32296:CDS 50.0%
!! AGAAGGTACCGAAGCCTATG+GGG + chr8.3:49168826-49168845 MS.gene32296:CDS 50.0%
!! GAAGAAGGTACCGAAGCCTA+TGG + chr8.3:49168824-49168843 MS.gene32296:CDS 50.0%
CACGGAGGAAACAAGCTGAG+AGG - chr8.3:49167811-49167830 None:intergenic 55.0%
CGATGATGCTCAGCACGATG+TGG - chr8.3:49167707-49167726 None:intergenic 55.0%
GAAAGCCAACGAAGGTCCTC+CGG - chr8.3:49167853-49167872 None:intergenic 55.0%
! AGGAGCAGCATTGGAGAGCA+CGG - chr8.3:49167829-49167848 None:intergenic 55.0%
!! ATCATCGCTGCTGGCTCTGA+AGG + chr8.3:49167720-49167739 MS.gene32296:CDS 55.0%
!! TTTATCGGTGGCGGAGTACG+AGG - chr8.3:49168887-49168906 None:intergenic 55.0%
CTCTCCAATGCTGCTCCTGC+CGG + chr8.3:49167831-49167850 MS.gene32296:CDS 60.0%
GTGCTGAGCATCATCGCTGC+TGG + chr8.3:49167711-49167730 MS.gene32296:CDS 60.0%
! AGCAGCATTGGAGAGCACGG+AGG - chr8.3:49167826-49167845 None:intergenic 60.0%
TCCAATGCTGCTCCTGCCGG+AGG + chr8.3:49167834-49167853 MS.gene32296:CDS 65.0%
TCCTCCGGCAGGAGCAGCAT+TGG - chr8.3:49167838-49167857 None:intergenic 65.0%
TCCTGCCGGAGGACCTTCGT+TGG + chr8.3:49167845-49167864 MS.gene32296:CDS 65.0%
GCCAACGAAGGTCCTCCGGC+AGG - chr8.3:49167849-49167868 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 49167585 49169003 49167585 ID=MS.gene32296
chr8.3 mRNA 49167585 49169003 49167585 ID=MS.gene32296.t1;Parent=MS.gene32296
chr8.3 exon 49167585 49167980 49167585 ID=MS.gene32296.t1.exon1;Parent=MS.gene32296.t1
chr8.3 CDS 49167585 49167980 49167585 ID=cds.MS.gene32296.t1;Parent=MS.gene32296.t1
chr8.3 exon 49168218 49169003 49168218 ID=MS.gene32296.t1.exon2;Parent=MS.gene32296.t1
chr8.3 CDS 49168218 49169003 49168218 ID=cds.MS.gene32296.t1;Parent=MS.gene32296.t1
Gene Sequence

>MS.gene32296

ATGAATTTTCAGAGCGAATCAATCACCAACCTAACCAAGGTTTCTTTGACCATCGCCAAACATTTGTTCTCCAAACAAGAATACAAAGAAACCAATGTTGTGTTTTCACCATTATCGCTCCACATCGTGCTGAGCATCATCGCTGCTGGCTCTGAAGGTCCTACACAAAAACAAGTTCTTTCATTCCTTCAATCCAAATCCACGGAAAATCTAAACAACTTTGCCTCTCAGCTTGTTTCCTCCGTGCTCTCCAATGCTGCTCCTGCCGGAGGACCTTCGTTGGCTTTCCTTAATGGTATGTGGGTTGAACAGACACTTTCTCTTGAACCTTCCTTCCGAAAAATTGTGGCTACCGATTTCAAAACTAATATTGCTTCAGTTGATTTCGTTAACAAGGTAAAATAATCTACAATCCTTTTTTTTATTTATCTTGAAGCCATATCGTGCTTTTATTATTTTATGTGGTATTTTATTTTGGTTGCTAACACTTCAATATTATTTGATAAAAGAATTAGGGTACCAAGTGGCCTAAATATCGATCTTTGTTCTTGTTTCATTCTATTAAAATAAGTTGTTACTAATTTCAATTGATCATTTTGCACCGTTAATTTTATGTTTGATATTGTTTTAAAGGCTCTCGAAGTGACAAAAGAAGCAAATTTATGGGCTGAAAAAGAGACAAATGGTCTTGTAAAAAATCTACTTTCACCTGAGTCATTTAATAGTTTAACCAACCTCATCTTTGCAAATGCACTATACTTTAAAGGAGTGTGGAAAGAGGAGTTTGATACTTCAGATACAAGAGACTATGATTTTCACATTCTCAATGGTAGCCCGATCAAGGTTCCCTTCATGACCAGCTATGAGATGCAGTATATCAGTGTTCTTGATGGTTTTAAAGTCCTTCGTCTTCCTTATACGCAAGGTGAAGACAAACGTCGATTCTCTATTTACTTTTTTCTTCCTGATGCAAAAGATGGATTGTTAGCTTTGATTAAGAAGCTAGCTTCCGAATCTAAGTTCTTGGAACACAAGCTTCCTCGCGAATTAGTGGAAGTTGGTGCTTTCAGGATTCCGAAATTCAAGATTTCTTTTGGGCTTGAATGTACTGATATGCTAAAAGAGCTAGGCGTGGTTTTACCTTTCTCCGAAGGAGCTTTGACAAAAATGGTGGACTCTCGTTTTGCTCAAAACCTTTATGTGTCCGACATATTTCACAAGTCTTTTATTGAAGTAAACGAAGAAGGTACCGAAGCCTATGGGGAAAGCTATATGGGGACAAGTTTGTGTGATGATTCTCCTCGTACTCCGCCACCGATAAACTTCGTAGTTGATCACCCTTTCTTGTTTTTGATCAGAGAAGATTTGTCTGGAACCATACTCTTTATTGGGCAAGTGCTCAATCCTCTTGACAAGTGA
Protein sequence

>MS.gene32296.t1

MNFQSESITNLTKVSLTIAKHLFSKQEYKETNVVFSPLSLHIVLSIIAAGSEGPTQKQVLSFLQSKSTENLNNFASQLVSSVLSNAAPAGGPSLAFLNGMWVEQTLSLEPSFRKIVATDFKTNIASVDFVNKALEVTKEANLWAEKETNGLVKNLLSPESFNSLTNLIFANALYFKGVWKEEFDTSDTRDYDFHILNGSPIKVPFMTSYEMQYISVLDGFKVLRLPYTQGEDKRRFSIYFFLPDAKDGLLALIKKLASESKFLEHKLPRELVEVGAFRIPKFKISFGLECTDMLKELGVVLPFSEGALTKMVDSRFAQNLYVSDIFHKSFIEVNEEGTEAYGESYMGTSLCDDSPRTPPPINFVVDHPFLFLIREDLSGTILFIGQVLNPLDK