Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32862.t1 | KEH35837.1 | 94.4 | 108 | 2 | 1 | 1 | 108 | 1 | 104 | 1.90E-46 | 194.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32862.t1 | A0A072V1Y9 | 94.4 | 108 | 2 | 1 | 1 | 108 | 1 | 104 | 1.4e-46 | 194.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053538 | MS.gene32862 | 0.802853 | 4.61E-49 | -1.69E-46 |
| MS.gene059424 | MS.gene32862 | 0.802044 | 6.76E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32862.t1 | MTR_3g103330 | 94.444 | 108 | 2 | 1 | 1 | 108 | 1 | 104 | 1.04e-69 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCGAGTCAAAGCTCTTC+TGG | 0.318499 | 3.1:+81810144 | MS.gene32862:CDS |
| CGCCATGACGGTGGTGTAGC+TGG | 0.367268 | 3.1:+81810327 | MS.gene32862:CDS |
| GGTTGCAGTGGAAAGCTTGA+TGG | 0.410694 | 3.1:+81810165 | MS.gene32862:CDS |
| TTTACCGGTCCTTCTCCTGC+TGG | 0.444782 | 3.1:-81810355 | None:intergenic |
| GCAGGAGAAGGACCGGTAAA+GGG | 0.444911 | 3.1:+81810358 | MS.gene32862:CDS |
| AGCAGGAGAAGGACCGGTAA+AGG | 0.449501 | 3.1:+81810357 | MS.gene32862:CDS |
| GAAACCACTGCAGGTGGAAG+AGG | 0.474000 | 3.1:-81810097 | None:intergenic |
| TTCATCTAGATCATTGTTCG+TGG | 0.475117 | 3.1:-81810398 | None:intergenic |
| GAACATCAATGGCAACTCAT+TGG | 0.481610 | 3.1:-81810289 | None:intergenic |
| GTTGATCAATGGTGTCACTA+TGG | 0.489333 | 3.1:+81810200 | MS.gene32862:CDS |
| GTGTAGCTGGTTCCACCAGC+AGG | 0.495334 | 3.1:+81810340 | MS.gene32862:CDS |
| ACCGGTCCTTCTCCTGCTGG+TGG | 0.523534 | 3.1:-81810352 | None:intergenic |
| TTACCATCGTTGAACATCAA+TGG | 0.525954 | 3.1:-81810300 | None:intergenic |
| ATCTTCTCCATCTTCTTCTG+TGG | 0.543326 | 3.1:-81810265 | None:intergenic |
| ACTACCTCTTCCACCTGCAG+TGG | 0.560557 | 3.1:+81810093 | MS.gene32862:CDS |
| CAAAGCTCTTCTGGTTGCAG+TGG | 0.566167 | 3.1:+81810153 | MS.gene32862:CDS |
| TCCACCAGCAGGAGAAGGAC+CGG | 0.569119 | 3.1:+81810351 | MS.gene32862:CDS |
| CGCATCGCCACAGAAGAAGA+TGG | 0.574444 | 3.1:+81810258 | MS.gene32862:CDS |
| CTGGTTCCACCAGCAGGAGA+AGG | 0.578852 | 3.1:+81810346 | MS.gene32862:CDS |
| TCGAAGTATTCGATGATGAT+CGG | 0.586920 | 3.1:-81810128 | None:intergenic |
| GTAGCTATGTGGTTGATCAA+TGG | 0.596285 | 3.1:+81810189 | MS.gene32862:CDS |
| GAAGTTGAAGAAACCACTGC+AGG | 0.609331 | 3.1:-81810106 | None:intergenic |
| GTTGAAGAAACCACTGCAGG+TGG | 0.625992 | 3.1:-81810103 | None:intergenic |
| GGTAATCTCCGCCATGACGG+TGG | 0.628227 | 3.1:+81810318 | MS.gene32862:CDS |
| AACCAGCTACACCACCGTCA+TGG | 0.629069 | 3.1:-81810329 | None:intergenic |
| GATGGTAATCTCCGCCATGA+CGG | 0.678032 | 3.1:+81810315 | MS.gene32862:CDS |
| AGCTTGATGGAGTAGCTATG+TGG | 0.699449 | 3.1:+81810178 | MS.gene32862:CDS |
| CAGCTACACCACCGTCATGG+CGG | 0.723788 | 3.1:-81810326 | None:intergenic |
| TTGCCATTGATGTTCAACGA+TGG | 0.744804 | 3.1:+81810297 | MS.gene32862:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTACCATCGTTGAACATCAA+TGG | - | chr3.1:81810303-81810322 | None:intergenic | 35.0% | |
| ! | TACACTTTTCTTCCCTTTAC+CGG | - | chr3.1:81810373-81810392 | None:intergenic | 35.0% |
| ! | TCGAAGTATTCGATGATGAT+CGG | - | chr3.1:81810131-81810150 | None:intergenic | 35.0% |
| ATCTTCTCCATCTTCTTCTG+TGG | - | chr3.1:81810268-81810287 | None:intergenic | 40.0% | |
| GAACATCAATGGCAACTCAT+TGG | - | chr3.1:81810292-81810311 | None:intergenic | 40.0% | |
| GTAGCTATGTGGTTGATCAA+TGG | + | chr3.1:81810189-81810208 | MS.gene32862:CDS | 40.0% | |
| TTGCCATTGATGTTCAACGA+TGG | + | chr3.1:81810297-81810316 | MS.gene32862:CDS | 40.0% | |
| !! | GTTGATCAATGGTGTCACTA+TGG | + | chr3.1:81810200-81810219 | MS.gene32862:CDS | 40.0% |
| ACTTCGAGTCAAAGCTCTTC+TGG | + | chr3.1:81810144-81810163 | MS.gene32862:CDS | 45.0% | |
| AGCTTGATGGAGTAGCTATG+TGG | + | chr3.1:81810178-81810197 | MS.gene32862:CDS | 45.0% | |
| GAAGTTGAAGAAACCACTGC+AGG | - | chr3.1:81810109-81810128 | None:intergenic | 45.0% | |
| GATGGTAATCTCCGCCATGA+CGG | + | chr3.1:81810315-81810334 | MS.gene32862:CDS | 50.0% | |
| GGTTGCAGTGGAAAGCTTGA+TGG | + | chr3.1:81810165-81810184 | MS.gene32862:CDS | 50.0% | |
| GTTGAAGAAACCACTGCAGG+TGG | - | chr3.1:81810106-81810125 | None:intergenic | 50.0% | |
| ! | CAAAGCTCTTCTGGTTGCAG+TGG | + | chr3.1:81810153-81810172 | MS.gene32862:CDS | 50.0% |
| AACCAGCTACACCACCGTCA+TGG | - | chr3.1:81810332-81810351 | None:intergenic | 55.0% | |
| ACTACCTCTTCCACCTGCAG+TGG | + | chr3.1:81810093-81810112 | MS.gene32862:CDS | 55.0% | |
| AGCAGGAGAAGGACCGGTAA+AGG | + | chr3.1:81810357-81810376 | MS.gene32862:CDS | 55.0% | |
| CGCATCGCCACAGAAGAAGA+TGG | + | chr3.1:81810258-81810277 | MS.gene32862:CDS | 55.0% | |
| GAAACCACTGCAGGTGGAAG+AGG | - | chr3.1:81810100-81810119 | None:intergenic | 55.0% | |
| GCAGGAGAAGGACCGGTAAA+GGG | + | chr3.1:81810358-81810377 | MS.gene32862:CDS | 55.0% | |
| TTTACCGGTCCTTCTCCTGC+TGG | - | chr3.1:81810358-81810377 | None:intergenic | 55.0% | |
| CAGCTACACCACCGTCATGG+CGG | - | chr3.1:81810329-81810348 | None:intergenic | 60.0% | |
| CTGGTTCCACCAGCAGGAGA+AGG | + | chr3.1:81810346-81810365 | MS.gene32862:CDS | 60.0% | |
| GGTAATCTCCGCCATGACGG+TGG | + | chr3.1:81810318-81810337 | MS.gene32862:CDS | 60.0% | |
| TCCACCAGCAGGAGAAGGAC+CGG | + | chr3.1:81810351-81810370 | MS.gene32862:CDS | 60.0% | |
| ! | GTGTAGCTGGTTCCACCAGC+AGG | + | chr3.1:81810340-81810359 | MS.gene32862:CDS | 60.0% |
| ACCGGTCCTTCTCCTGCTGG+TGG | - | chr3.1:81810355-81810374 | None:intergenic | 65.0% | |
| CGCCATGACGGTGGTGTAGC+TGG | + | chr3.1:81810327-81810346 | MS.gene32862:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 81810084 | 81810410 | 81810084 | ID=MS.gene32862 |
| chr3.1 | mRNA | 81810084 | 81810410 | 81810084 | ID=MS.gene32862.t1;Parent=MS.gene32862 |
| chr3.1 | exon | 81810084 | 81810410 | 81810084 | ID=MS.gene32862.t1.exon1;Parent=MS.gene32862.t1 |
| chr3.1 | CDS | 81810084 | 81810410 | 81810084 | ID=cds.MS.gene32862.t1;Parent=MS.gene32862.t1 |
| Gene Sequence |
| Protein sequence |
