AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32890.t1 MTR_3g102920 85.779 443 28 3 1 442 1 409 0.0 742
MS.gene32890.t1 MTR_4g045707 65.206 388 127 4 57 441 4 386 1.80e-173 491
MS.gene32890.t1 MTR_3g101010 68.865 379 110 4 67 441 13 387 1.05e-172 489
MS.gene32890.t1 MTR_3g048000 62.740 416 121 7 32 443 7 392 1.56e-171 486
MS.gene32890.t1 MTR_3g018740 64.377 393 126 5 52 441 5 386 7.25e-169 479
MS.gene32890.t1 MTR_8g027395 62.129 404 141 6 42 442 2 396 8.87e-164 467
MS.gene32890.t1 MTR_7g451890 58.706 402 150 4 54 441 1 400 2.45e-162 464
MS.gene32890.t1 MTR_3g101030 59.427 419 142 6 47 441 13 427 5.78e-161 461
MS.gene32890.t1 MTR_8g461160 62.821 390 131 4 63 443 10 394 1.72e-160 459
MS.gene32890.t1 MTR_3g101130 59.898 394 147 3 54 442 1 388 2.73e-159 456
MS.gene32890.t1 MTR_7g047780 58.955 402 149 4 54 441 1 400 8.68e-159 454
MS.gene32890.t1 MTR_7g050750 61.039 385 142 3 63 441 12 394 4.55e-158 452
MS.gene32890.t1 MTR_7g050810 58.680 409 152 6 41 441 3 402 2.53e-156 448
MS.gene32890.t1 MTR_3g101180 59.288 393 146 4 63 443 10 400 2.92e-153 441
MS.gene32890.t1 MTR_4g088525 62.810 363 127 3 83 442 11 368 1.69e-152 437
MS.gene32890.t1 MTR_8g027420 60.789 380 128 5 66 442 13 374 7.17e-152 436
MS.gene32890.t1 MTR_2g046030 61.880 383 131 6 66 441 12 386 8.12e-152 436
MS.gene32890.t1 MTR_2g045980 60.836 383 135 6 66 441 12 386 3.05e-148 427
MS.gene32890.t1 MTR_4g088535 57.289 391 141 6 55 442 3 370 4.21e-142 411
MS.gene32890.t1 MTR_3g101050 59.115 384 128 6 67 443 37 398 6.68e-140 406
MS.gene32890.t1 MTR_3g015620 57.105 380 135 4 66 441 23 378 3.28e-135 394
MS.gene32890.t1 MTR_3g101200 52.381 399 149 8 57 443 23 392 1.56e-117 349
MS.gene32890.t1 MTR_3g100995 64.062 256 85 2 189 441 11 262 4.94e-112 330
MS.gene32890.t1 MTR_7g050830 54.723 307 108 2 67 353 24 319 7.45e-111 330
MS.gene32890.t1 MTR_3g101190 49.180 366 119 4 80 442 10 311 9.97e-111 329
MS.gene32890.t1 MTR_3g100010 53.398 309 96 2 81 356 37 330 2.07e-105 317
MS.gene32890.t1 MTR_3g018747 63.077 260 78 3 185 441 9 253 2.97e-104 310
MS.gene32890.t1 MTR_3g015760 55.357 280 109 4 170 441 8 279 4.22e-96 290
MS.gene32890.t1 MTR_3g111160 63.636 220 74 4 226 441 1 218 5.53e-82 252
MS.gene32890.t1 MTR_3g114310 42.781 374 194 11 83 440 38 407 1.35e-81 258
MS.gene32890.t1 MTR_3g100520 58.824 221 74 4 226 442 1 208 3.39e-78 242
MS.gene32890.t1 MTR_2g046410 62.802 207 67 4 242 441 144 347 9.72e-76 240
MS.gene32890.t1 MTR_3g099970 63.636 198 50 5 185 379 20 198 1.12e-73 230
MS.gene32890.t1 MTR_3g101110 54.357 241 76 4 188 421 71 284 1.96e-71 227
MS.gene32890.t1 MTR_3g101020 54.645 183 58 1 259 441 1 158 3.13e-57 186
MS.gene32890.t1 MTR_3g101150 60.625 160 53 4 291 442 1 158 1.68e-52 174
MS.gene32890.t1 MTR_3g102870 58.861 158 60 2 248 402 1 156 5.16e-52 173
MS.gene32890.t1 MTR_3g111150 50.575 174 60 3 66 239 6 153 1.12e-49 167
MS.gene32890.t1 MTR_3g100990 57.664 137 49 2 50 186 3 130 6.75e-41 152
MS.gene32890.t1 MTR_4g093550 48.352 91 45 1 54 144 1 89 2.21e-19 83.2
MS.gene32890.t1 MTR_3g101140 46.667 120 48 2 324 441 9 114 1.34e-18 81.6
MS.gene32890.t1 MTR_6g046890 61.765 68 26 0 76 143 14 81 2.04e-18 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32890.t1 AT1G47710 49.081 381 187 4 66 441 12 390 3.23e-126 371
MS.gene32890.t1 AT1G47710 46.667 420 199 6 27 441 18 417 5.86e-126 372
MS.gene32890.t1 AT2G25240 46.114 386 190 8 61 440 9 382 6.18e-108 324
MS.gene32890.t1 AT3G45220 46.175 366 182 6 85 440 30 390 8.23e-106 319
MS.gene32890.t1 AT2G26390 44.386 383 195 6 67 440 13 386 1.03e-102 311
MS.gene32890.t1 AT1G62170 40.884 362 187 3 85 440 91 431 6.04e-91 283
MS.gene32890.t1 AT2G14540 42.105 380 193 5 66 440 40 397 8.05e-89 276
MS.gene32890.t1 AT1G64030 41.842 380 194 7 67 440 13 371 8.70e-87 270
MS.gene32890.t1 AT1G62170 37.468 395 187 4 85 440 91 464 8.68e-84 265
MS.gene32890.t1 AT2G35580 41.690 355 183 6 92 440 35 371 1.70e-81 256
MS.gene32890.t1 AT1G64010 44.444 216 98 4 226 440 1 195 1.35e-53 178
MS.gene32890.t1 AT1G64010 44.279 201 90 4 241 440 2 181 4.54e-47 160
MS.gene32890.t1 AT1G63280 51.304 115 52 2 185 297 8 120 1.07e-29 112
MS.gene32890.t1 AT1G62160 40.110 182 86 2 261 440 58 218 1.95e-28 112
MS.gene32890.t1 AT1G51330 46.847 111 56 2 184 291 30 140 2.53e-24 100
MS.gene32890.t1 AT1G64020 36.036 111 68 3 66 175 12 120 4.95e-13 66.2

Find 112 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGCCATTTGTCTCCTTAT+TGG 0.204637 3.1:+81450566 None:intergenic
AGCTTTCTCTTTGACTAAAA+TGG 0.229511 3.1:-81450094 MS.gene32890:CDS
AATGGAGAGAAGCAGTTTAT+TGG 0.238977 3.1:-81450375 MS.gene32890:CDS
TGAAAGACTTGTGAAATATC+TGG 0.264230 3.1:+81450031 None:intergenic
AATCGTCTCTACTGATTATA+AGG 0.274934 3.1:-81450933 MS.gene32890:CDS
GTATCTTCACCTTTGTTATA+AGG 0.300319 3.1:+81450321 None:intergenic
GACAGTCCATCTCTTCGATT+TGG 0.307963 3.1:+81450270 None:intergenic
TCAAATGAAACCTTGAATCT+TGG 0.321190 3.1:+81450159 None:intergenic
CGGCGGCTCCGGTGAGGGCG+AGG 0.349388 3.1:+81451412 None:intergenic
TCTGATCTCCTGAAGGAGTT+AGG 0.359345 3.1:-81450126 MS.gene32890:CDS
TGAGCACATGTCCAACAAAA+AGG 0.363030 3.1:+81449863 None:intergenic
GAAATATCTGGGAAACAAAA+TGG 0.364280 3.1:+81450043 None:intergenic
TGTGAATTCATGGGCCAATA+AGG 0.384788 3.1:-81450580 MS.gene32890:CDS
AACCCACTCCTAACTCCTTC+AGG 0.386430 3.1:+81450118 None:intergenic
GAGGAAGAAATTTCTCAATA+AGG 0.390097 3.1:+81450547 None:intergenic
TGAAGGAAGGTTGAAGAGAA+AGG 0.399705 3.1:+81450960 None:intergenic
GAATCAACAATTTGATGCTT+CGG 0.418605 3.1:-81450460 MS.gene32890:CDS
CTCCATCCCCTCGCCCTCAC+CGG 0.418960 3.1:-81451420 MS.gene32890:CDS
CATCATCGCGGCTGGCTCTG+AGG 0.420947 3.1:-81451140 MS.gene32890:CDS
AGTAGAGACGATTTGTTTGA+AGG 0.421065 3.1:+81450943 None:intergenic
CTAATGCACTGTACTTCAAA+GGG 0.430202 3.1:-81450488 MS.gene32890:CDS
AAGAGATGGACTGTCAGCTT+TGG 0.434375 3.1:-81450262 MS.gene32890:CDS
GAGAGATGCTGCGGCGGCTC+CGG 0.435055 3.1:+81451401 None:intergenic
ATTGAGAAATTTCTTCCTCC+AGG 0.436586 3.1:-81450543 MS.gene32890:CDS
CTCTCGTTCTCCTGTGCCAC+GGG 0.438774 3.1:-81451323 MS.gene32890:CDS
AAATGGTCTTGATCTTGATC+TGG 0.440586 3.1:+81450060 None:intergenic
CAAAAGCTTCTCGACTTCCT+TGG 0.441120 3.1:-81451106 MS.gene32890:CDS
GGCGGCTCCGGTGAGGGCGA+GGG 0.441327 3.1:+81451413 None:intergenic
GAAGGAAGGTTGAAGAGAAA+GGG 0.442090 3.1:+81450961 None:intergenic
CTCCGGTGAGGGCGAGGGGA+TGG 0.447953 3.1:+81451418 None:intergenic
GTTCTTGTTTGGAGAGCAAA+TGG 0.450572 3.1:+81451215 None:intergenic
GTGCTCAGCATCATCGCGGC+TGG 0.453046 3.1:-81451148 MS.gene32890:CDS
CGGTTGTAAGAAGGAGGTGG+AGG 0.454172 3.1:+81451346 None:intergenic
TGCGGCTGGTCCCGTGGCAC+AGG 0.459049 3.1:+81451313 None:intergenic
CTTCTCTCCATTGGTCATGA+AGG 0.460859 3.1:+81450386 None:intergenic
GAGACGATTTGTTTGAAGGA+AGG 0.460863 3.1:+81450947 None:intergenic
CGTCTTTCTAAAGTCAAAGT+AGG 0.462987 3.1:-81450195 MS.gene32890:CDS
TGCTGCGGCGGCTCCGGTGA+GGG 0.464534 3.1:+81451407 None:intergenic
ATCATCGCGGCTGGCTCTGA+GGG 0.469342 3.1:-81451139 MS.gene32890:CDS
TTTCAGAATTCCAAGATTCA+AGG 0.470578 3.1:-81450169 MS.gene32890:CDS
TGGTCATGGAAACCTTGATT+AGG 0.475061 3.1:+81451245 None:intergenic
GTCAGCTTTGGTTGAGAAGG+TGG 0.480324 3.1:-81450250 MS.gene32890:CDS
GAAAGACTTGTGAAATATCT+GGG 0.489574 3.1:+81450032 None:intergenic
ATGCTGCGGCGGCTCCGGTG+AGG 0.490256 3.1:+81451406 None:intergenic
AATAAACTGCTTCTCTCCAT+TGG 0.490699 3.1:+81450377 None:intergenic
GAGGGCGAGGGGATGGAGAA+TGG 0.491783 3.1:+81451425 None:intergenic
TGACGAATGATGTGAATTCA+TGG 0.493920 3.1:-81450590 MS.gene32890:CDS
GAGACGCAAGGCCCACTGGC+AGG 0.496918 3.1:+81451013 None:intergenic
TACCTTGTTCTTGAAATCTA+CGG 0.497341 3.1:+81450898 None:intergenic
GCGGCTCCGGTGAGGGCGAG+GGG 0.500491 3.1:+81451414 None:intergenic
GAACGAGAGCGGTTGTAAGA+AGG 0.500652 3.1:+81451337 None:intergenic
CAAATGGTTGGCCATGGTCA+TGG 0.504652 3.1:+81451231 None:intergenic
TCTCTAATTAGAAATAAGAA+AGG 0.509129 3.1:+81449904 None:intergenic
CGTTGCTTGTCTCGTGGCAG+AGG 0.512301 3.1:+81451379 None:intergenic
TTTCTTCCAAATCGAAGAGA+TGG 0.512506 3.1:-81450276 MS.gene32890:CDS
GCTAATGCACTGTACTTCAA+AGG 0.515272 3.1:-81450489 MS.gene32890:CDS
CACCTTTGTTATAAGGAAGA+CGG 0.517132 3.1:+81450328 None:intergenic
CACTGTACTTCAAAGGGGTG+TGG 0.520367 3.1:-81450482 MS.gene32890:CDS
CAAGGCCCACTGGCAGGAGA+AGG 0.528850 3.1:+81451019 None:intergenic
CTCTAATTAGAAATAAGAAA+GGG 0.536391 3.1:+81449905 None:intergenic
TGGGCCAATAAGGAGACAAA+TGG 0.540100 3.1:-81450570 MS.gene32890:CDS
TGAAAGGAGATGAGAGGCAA+AGG 0.542167 3.1:+81451057 None:intergenic
TTCATCGAAGTGAATGAAGA+AGG 0.545102 3.1:-81450012 MS.gene32890:CDS
TTCTCTCCATTGGTCATGAA+GGG 0.552364 3.1:+81450387 None:intergenic
GCTCTCGTTCTCCTGTGCCA+CGG 0.552729 3.1:-81451324 MS.gene32890:CDS
CGCTAAGAAAACTTAACAAG+AGG 0.552754 3.1:+81449838 None:intergenic
CTCCTGAAGGAGTTAGGAGT+GGG 0.552865 3.1:-81450120 MS.gene32890:CDS
TTGAGAAATTTCTTCCTCCA+GGG 0.555089 3.1:-81450542 MS.gene32890:CDS
CTCCGTCTTCCTTATAACAA+AGG 0.555897 3.1:-81450330 MS.gene32890:CDS
AATGATCCTTCTCCTGCCAG+TGG 0.556621 3.1:-81451025 MS.gene32890:CDS
AAAAGAGACGCAAGGCCCAC+TGG 0.559566 3.1:+81451009 None:intergenic
GTGAATTTGTTGACTAACCC+TGG 0.561723 3.1:+81450525 None:intergenic
GGAGAGCAAATGGTTGGCCA+TGG 0.562406 3.1:+81451225 None:intergenic
GAGCGGTTGTAAGAAGGAGG+TGG 0.563038 3.1:+81451343 None:intergenic
GATCATTGAGTACGGCTGAA+AGG 0.563262 3.1:+81451041 None:intergenic
AATGAGAATGTTGTTGCGGC+TGG 0.567626 3.1:+81451299 None:intergenic
AAGGTTCCCTTCATGACCAA+TGG 0.569018 3.1:-81450393 MS.gene32890:CDS
TGGAAACCTTGATTAGGTCA+TGG 0.569544 3.1:+81451251 None:intergenic
TTCCGTAGATTTCAAGAACA+AGG 0.570481 3.1:-81450900 MS.gene32890:intron
ATGATCCTTCTCCTGCCAGT+GGG 0.570580 3.1:-81451024 MS.gene32890:CDS
AGAAATTTCTGATCTCCTGA+AGG 0.571673 3.1:-81450133 MS.gene32890:CDS
TTGTTTGGAGAGCAAATGGT+TGG 0.575521 3.1:+81451219 None:intergenic
TTTCTCTTTGACTAAAATGG+TGG 0.577345 3.1:-81450091 MS.gene32890:CDS
TATGAACCATGACCTAATCA+AGG 0.578811 3.1:-81451257 MS.gene32890:CDS
GAAATCTACGGAAGACAAAG+TGG 0.582565 3.1:+81450910 None:intergenic
TGTTGTTGCGGCTGGTCCCG+TGG 0.588060 3.1:+81451307 None:intergenic
AGGAGAAGGATCATTGAGTA+CGG 0.591656 3.1:+81451033 None:intergenic
CTTTAGAAAGACGAAGCTTG+TGG 0.598117 3.1:+81450205 None:intergenic
AGCCGCGATGATGCTGAGCA+CGG 0.599331 3.1:+81451150 None:intergenic
AGAAGGAACTGAAGCTGCTG+CGG 0.604436 3.1:-81449995 MS.gene32890:CDS
ACTGTCAGCTTTGGTTGAGA+AGG 0.612453 3.1:-81450253 MS.gene32890:CDS
CGAGAGCGGTTGTAAGAAGG+AGG 0.618378 3.1:+81451340 None:intergenic
GCAGAGGAGAGATGCTGCGG+CGG 0.621598 3.1:+81451395 None:intergenic
TCATAATGGAAGCTCAGTCA+AGG 0.629742 3.1:-81450412 MS.gene32890:CDS
AAAGGAGTTGAGATGATCGA+TGG 0.632268 3.1:+81451075 None:intergenic
TCTCCTGAAGGAGTTAGGAG+TGG 0.638265 3.1:-81450121 MS.gene32890:CDS
GTGGCAGAGGAGAGATGCTG+CGG 0.653054 3.1:+81451392 None:intergenic
GACGAATGATGTGAATTCAT+GGG 0.653709 3.1:-81450589 MS.gene32890:CDS
TACGGCTGAAAGGAGATGAG+AGG 0.653894 3.1:+81451051 None:intergenic
AATCAAGGTTTCCATGACCA+TGG 0.656656 3.1:-81451242 MS.gene32890:CDS
CGATGATGCTGAGCACGGAG+TGG 0.656723 3.1:+81451155 None:intergenic
AGCACGGAGTGGATTAACAA+TGG 0.657807 3.1:+81451166 None:intergenic
TAATTAGAAATAAGAAAGGG+TGG 0.659871 3.1:+81449908 None:intergenic
GTGGCACAGGAGAACGAGAG+CGG 0.663796 3.1:+81451326 None:intergenic
AGAGAATGAGAATGTTGTTG+CGG 0.669476 3.1:+81451295 None:intergenic
CCATTGACAAAAGAGACGCA+AGG 0.678201 3.1:+81451001 None:intergenic
ATAACAAAGGTGAAGATACG+CGG 0.685150 3.1:-81450317 MS.gene32890:CDS
TCAGCTACAAAGTCTAGTCG+AGG 0.687215 3.1:+81449931 None:intergenic
TGATGACGTTGCTTGTCTCG+TGG 0.695779 3.1:+81451373 None:intergenic
TAATGCACTGTACTTCAAAG+GGG 0.705776 3.1:-81450487 MS.gene32890:CDS
CTCCGTGCTCAGCATCATCG+CGG 0.742024 3.1:-81451152 MS.gene32890:CDS
AATTTGTTGACTAACCCTGG+AGG 0.815735 3.1:+81450528 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GCATACTTTATTTTAAAATT+TGG - chr3.1:81450637-81450656 MS.gene32890:intron 15.0%
!! CTCTAATTAGAAATAAGAAA+GGG + chr3.1:81451371-81451390 None:intergenic 20.0%
!! TCTCTAATTAGAAATAAGAA+AGG + chr3.1:81451372-81451391 None:intergenic 20.0%
!!! AAAATTTGGTATGATTTTGT+AGG - chr3.1:81450651-81450670 MS.gene32890:intron 20.0%
!!! CTTTATTTTCTGTTAGTTAA+TGG - chr3.1:81450423-81450442 MS.gene32890:CDS 20.0%
!!! TATGATTTTCACATTCATAA+TGG - chr3.1:81450847-81450866 MS.gene32890:intron 20.0%
! AACTAACAGAAAATAAAGCA+TGG + chr3.1:81450421-81450440 None:intergenic 25.0%
! AATTGAAGCATATAATTCAG+AGG - chr3.1:81450509-81450528 MS.gene32890:CDS 25.0%
! TAATTAGAAATAAGAAAGGG+TGG + chr3.1:81451368-81451387 None:intergenic 25.0%
! TATTTCATAACATGGTTTAG+AGG - chr3.1:81450475-81450494 MS.gene32890:CDS 25.0%
!! GTGTACTTTATTTCATAACA+TGG - chr3.1:81450467-81450486 MS.gene32890:CDS 25.0%
AGCTTTCTCTTTGACTAAAA+TGG - chr3.1:81451179-81451198 MS.gene32890:CDS 30.0%
AGTACACTAATTGATTGTGA+AGG + chr3.1:81450454-81450473 None:intergenic 30.0%
GAAAGACTTGTGAAATATCT+GGG + chr3.1:81451244-81451263 None:intergenic 30.0%
GAAATATCTGGGAAACAAAA+TGG + chr3.1:81451233-81451252 None:intergenic 30.0%
GAATCAACAATTTGATGCTT+CGG - chr3.1:81450813-81450832 MS.gene32890:intron 30.0%
GAGGAAGAAATTTCTCAATA+AGG + chr3.1:81450729-81450748 None:intergenic 30.0%
GTTAGTAAAGAACGACTTAA+AGG + chr3.1:81450563-81450582 None:intergenic 30.0%
TACCTTGTTCTTGAAATCTA+CGG + chr3.1:81450378-81450397 None:intergenic 30.0%
TCAAATGAAACCTTGAATCT+TGG + chr3.1:81451117-81451136 None:intergenic 30.0%
TGAAAGACTTGTGAAATATC+TGG + chr3.1:81451245-81451264 None:intergenic 30.0%
TTTCAGAATTCCAAGATTCA+AGG - chr3.1:81451104-81451123 MS.gene32890:CDS 30.0%
TTTCTCTTTGACTAAAATGG+TGG - chr3.1:81451182-81451201 MS.gene32890:CDS 30.0%
! AATCGTCTCTACTGATTATA+AGG - chr3.1:81450340-81450359 MS.gene32890:CDS 30.0%
! GTATCTTCACCTTTGTTATA+AGG + chr3.1:81450955-81450974 None:intergenic 30.0%
!! CTTTTCTTCAAGTTCTTGTT+TGG + chr3.1:81450072-81450091 None:intergenic 30.0%
AATAAACTGCTTCTCTCCAT+TGG + chr3.1:81450899-81450918 None:intergenic 35.0%
AATGGAGAGAAGCAGTTTAT+TGG - chr3.1:81450898-81450917 MS.gene32890:intron 35.0%
AGAAATTTCTGATCTCCTGA+AGG - chr3.1:81451140-81451159 MS.gene32890:CDS 35.0%
AGAGAATGAGAATGTTGTTG+CGG + chr3.1:81449981-81450000 None:intergenic 35.0%
ATAACAAAGGTGAAGATACG+CGG - chr3.1:81450956-81450975 MS.gene32890:CDS 35.0%
ATTGAGAAATTTCTTCCTCC+AGG - chr3.1:81450730-81450749 MS.gene32890:intron 35.0%
CACTAATTGATTGTGAAGGT+AGG + chr3.1:81450450-81450469 None:intergenic 35.0%
CTAATGCACTGTACTTCAAA+GGG - chr3.1:81450785-81450804 MS.gene32890:intron 35.0%
GACGAATGATGTGAATTCAT+GGG - chr3.1:81450684-81450703 MS.gene32890:intron 35.0%
TAATGCACTGTACTTCAAAG+GGG - chr3.1:81450786-81450805 MS.gene32890:intron 35.0%
TATGAACCATGACCTAATCA+AGG - chr3.1:81450016-81450035 MS.gene32890:CDS 35.0%
TCCAACAAAAAGGATCGTTT+CGG + chr3.1:81451403-81451422 None:intergenic 35.0%
TGACGAATGATGTGAATTCA+TGG - chr3.1:81450683-81450702 MS.gene32890:intron 35.0%
TTCATCGAAGTGAATGAAGA+AGG - chr3.1:81451261-81451280 MS.gene32890:CDS 35.0%
TTCCGTAGATTTCAAGAACA+AGG - chr3.1:81450373-81450392 MS.gene32890:CDS 35.0%
TTGAGAAATTTCTTCCTCCA+GGG - chr3.1:81450731-81450750 MS.gene32890:intron 35.0%
TTTCTTCCAAATCGAAGAGA+TGG - chr3.1:81450997-81451016 MS.gene32890:CDS 35.0%
! AAATGGTCTTGATCTTGATC+TGG + chr3.1:81451216-81451235 None:intergenic 35.0%
! AGTAGAGACGATTTGTTTGA+AGG + chr3.1:81450333-81450352 None:intergenic 35.0%
! CACCTTTGTTATAAGGAAGA+CGG + chr3.1:81450948-81450967 None:intergenic 35.0%
!! CGTCTTTCTAAAGTCAAAGT+AGG - chr3.1:81451078-81451097 MS.gene32890:CDS 35.0%
AAAGGAGTTGAGATGATCGA+TGG + chr3.1:81450201-81450220 None:intergenic 40.0%
AAGGCCATTTGTCTCCTTAT+TGG + chr3.1:81450710-81450729 None:intergenic 40.0%
AATCAAGGTTTCCATGACCA+TGG - chr3.1:81450031-81450050 MS.gene32890:CDS 40.0%
AGGAGAAGGATCATTGAGTA+CGG + chr3.1:81450243-81450262 None:intergenic 40.0%
CTCCGTCTTCCTTATAACAA+AGG - chr3.1:81450943-81450962 MS.gene32890:CDS 40.0%
GAAATCTACGGAAGACAAAG+TGG + chr3.1:81450366-81450385 None:intergenic 40.0%
GAAGGAAGGTTGAAGAGAAA+GGG + chr3.1:81450315-81450334 None:intergenic 40.0%
GCTAATGCACTGTACTTCAA+AGG - chr3.1:81450784-81450803 MS.gene32890:intron 40.0%
TGAAGGAAGGTTGAAGAGAA+AGG + chr3.1:81450316-81450335 None:intergenic 40.0%
TGAGCACATGTCCAACAAAA+AGG + chr3.1:81451413-81451432 None:intergenic 40.0%
TGGAAACCTTGATTAGGTCA+TGG + chr3.1:81450025-81450044 None:intergenic 40.0%
TGGTCATGGAAACCTTGATT+AGG + chr3.1:81450031-81450050 None:intergenic 40.0%
TGTGAATTCATGGGCCAATA+AGG - chr3.1:81450693-81450712 MS.gene32890:intron 40.0%
TTCTCTCCATTGGTCATGAA+GGG + chr3.1:81450889-81450908 None:intergenic 40.0%
TTGTTTGGAGAGCAAATGGT+TGG + chr3.1:81450057-81450076 None:intergenic 40.0%
! ACCGAAACGATCCTTTTTGT+TGG - chr3.1:81451399-81451418 MS.gene32890:CDS 40.0%
! CTTGCGTCTCTTTTGTCAAT+GGG - chr3.1:81450273-81450292 MS.gene32890:CDS 40.0%
! CTTTAGAAAGACGAAGCTTG+TGG + chr3.1:81451071-81451090 None:intergenic 40.0%
! GAGACGATTTGTTTGAAGGA+AGG + chr3.1:81450329-81450348 None:intergenic 40.0%
! GTTCTTGTTTGGAGAGCAAA+TGG + chr3.1:81450061-81450080 None:intergenic 40.0%
! TCATAATGGAAGCTCAGTCA+AGG - chr3.1:81450861-81450880 MS.gene32890:intron 40.0%
!! AATTTGTTGACTAACCCTGG+AGG + chr3.1:81450748-81450767 None:intergenic 40.0%
!! GTGAATTTGTTGACTAACCC+TGG + chr3.1:81450751-81450770 None:intergenic 40.0%
!!! CTCTTTTGTCAATGGGCTAT+GGG - chr3.1:81450280-81450299 MS.gene32890:CDS 40.0%
!!! TCTCTTTTGTCAATGGGCTA+TGG - chr3.1:81450279-81450298 MS.gene32890:CDS 40.0%
AAGGTTCCCTTCATGACCAA+TGG - chr3.1:81450880-81450899 MS.gene32890:intron 45.0%
AATGAGAATGTTGTTGCGGC+TGG + chr3.1:81449977-81449996 None:intergenic 45.0%
CAAAAGCTTCTCGACTTCCT+TGG - chr3.1:81450167-81450186 MS.gene32890:CDS 45.0%
CCATTGACAAAAGAGACGCA+AGG + chr3.1:81450275-81450294 None:intergenic 45.0%
CTTCTCTCCATTGGTCATGA+AGG + chr3.1:81450890-81450909 None:intergenic 45.0%
GACAGTCCATCTCTTCGATT+TGG + chr3.1:81451006-81451025 None:intergenic 45.0%
GATCATTGAGTACGGCTGAA+AGG + chr3.1:81450235-81450254 None:intergenic 45.0%
TCAGCTACAAAGTCTAGTCG+AGG + chr3.1:81451345-81451364 None:intergenic 45.0%
TCTGATCTCCTGAAGGAGTT+AGG - chr3.1:81451147-81451166 MS.gene32890:CDS 45.0%
TGAAAGGAGATGAGAGGCAA+AGG + chr3.1:81450219-81450238 None:intergenic 45.0%
TGGGCCAATAAGGAGACAAA+TGG - chr3.1:81450703-81450722 MS.gene32890:intron 45.0%
! ACTGTCAGCTTTGGTTGAGA+AGG - chr3.1:81451020-81451039 MS.gene32890:CDS 45.0%
! CCTTGCGTCTCTTTTGTCAA+TGG - chr3.1:81450272-81450291 MS.gene32890:CDS 45.0%
!! AAGAGATGGACTGTCAGCTT+TGG - chr3.1:81451011-81451030 MS.gene32890:CDS 45.0%
!! AGCACGGAGTGGATTAACAA+TGG + chr3.1:81450110-81450129 None:intergenic 45.0%
!! GATCGATGGATTTTGAGCCA+AGG + chr3.1:81450187-81450206 None:intergenic 45.0%
!! TCGAGAAGCTTTTGCTGTGT+GGG + chr3.1:81450161-81450180 None:intergenic 45.0%
AACCCACTCCTAACTCCTTC+AGG + chr3.1:81451158-81451177 None:intergenic 50.0%
AATGATCCTTCTCCTGCCAG+TGG - chr3.1:81450248-81450267 MS.gene32890:CDS 50.0%
AGAAGGAACTGAAGCTGCTG+CGG - chr3.1:81451278-81451297 MS.gene32890:CDS 50.0%
ATGATCCTTCTCCTGCCAGT+GGG - chr3.1:81450249-81450268 MS.gene32890:CDS 50.0%
CACTGTACTTCAAAGGGGTG+TGG - chr3.1:81450791-81450810 MS.gene32890:intron 50.0%
CTCCTGAAGGAGTTAGGAGT+GGG - chr3.1:81451153-81451172 MS.gene32890:CDS 50.0%
GAACGAGAGCGGTTGTAAGA+AGG + chr3.1:81449939-81449958 None:intergenic 50.0%
TACGGCTGAAAGGAGATGAG+AGG + chr3.1:81450225-81450244 None:intergenic 50.0%
TCTCCTGAAGGAGTTAGGAG+TGG - chr3.1:81451152-81451171 MS.gene32890:CDS 50.0%
! CAAATGGTTGGCCATGGTCA+TGG + chr3.1:81450045-81450064 None:intergenic 50.0%
! GTCAGCTTTGGTTGAGAAGG+TGG - chr3.1:81451023-81451042 MS.gene32890:CDS 50.0%
! TGATGACGTTGCTTGTCTCG+TGG + chr3.1:81449903-81449922 None:intergenic 50.0%
!! GTCGAGAAGCTTTTGCTGTG+TGG + chr3.1:81450162-81450181 None:intergenic 50.0%
AAAAGAGACGCAAGGCCCAC+TGG + chr3.1:81450267-81450286 None:intergenic 55.0%
CGAGAGCGGTTGTAAGAAGG+AGG + chr3.1:81449936-81449955 None:intergenic 55.0%
CGGTTGTAAGAAGGAGGTGG+AGG + chr3.1:81449930-81449949 None:intergenic 55.0%
GAGCGGTTGTAAGAAGGAGG+TGG + chr3.1:81449933-81449952 None:intergenic 55.0%
! GGAGAGCAAATGGTTGGCCA+TGG + chr3.1:81450051-81450070 None:intergenic 55.0%
AGCCGCGATGATGCTGAGCA+CGG + chr3.1:81450126-81450145 None:intergenic 60.0%
CGATGATGCTGAGCACGGAG+TGG + chr3.1:81450121-81450140 None:intergenic 60.0%
CGTTGCTTGTCTCGTGGCAG+AGG + chr3.1:81449897-81449916 None:intergenic 60.0%
CTCCGTGCTCAGCATCATCG+CGG - chr3.1:81450121-81450140 MS.gene32890:CDS 60.0%
CTCTCGTTCTCCTGTGCCAC+GGG - chr3.1:81449950-81449969 MS.gene32890:CDS 60.0%
GCTCTCGTTCTCCTGTGCCA+CGG - chr3.1:81449949-81449968 MS.gene32890:CDS 60.0%
GTGGCACAGGAGAACGAGAG+CGG + chr3.1:81449950-81449969 None:intergenic 60.0%
GTGGCAGAGGAGAGATGCTG+CGG + chr3.1:81449884-81449903 None:intergenic 60.0%
!! ATCATCGCGGCTGGCTCTGA+GGG - chr3.1:81450134-81450153 MS.gene32890:CDS 60.0%
CAAGGCCCACTGGCAGGAGA+AGG + chr3.1:81450257-81450276 None:intergenic 65.0%
GAGGGCGAGGGGATGGAGAA+TGG + chr3.1:81449851-81449870 None:intergenic 65.0%
GCAGAGGAGAGATGCTGCGG+CGG + chr3.1:81449881-81449900 None:intergenic 65.0%
GTGCTCAGCATCATCGCGGC+TGG - chr3.1:81450125-81450144 MS.gene32890:CDS 65.0%
! TGTTGTTGCGGCTGGTCCCG+TGG + chr3.1:81449969-81449988 None:intergenic 65.0%
!! CATCATCGCGGCTGGCTCTG+AGG - chr3.1:81450133-81450152 MS.gene32890:CDS 65.0%
CTCCATCCCCTCGCCCTCAC+CGG - chr3.1:81449853-81449872 MS.gene32890:CDS 70.0%
GAGACGCAAGGCCCACTGGC+AGG + chr3.1:81450263-81450282 None:intergenic 70.0%
GAGAGATGCTGCGGCGGCTC+CGG + chr3.1:81449875-81449894 None:intergenic 70.0%
ATGCTGCGGCGGCTCCGGTG+AGG + chr3.1:81449870-81449889 None:intergenic 75.0%
CTCCGGTGAGGGCGAGGGGA+TGG + chr3.1:81449858-81449877 None:intergenic 75.0%
TGCTGCGGCGGCTCCGGTGA+GGG + chr3.1:81449869-81449888 None:intergenic 75.0%
! TGCGGCTGGTCCCGTGGCAC+AGG + chr3.1:81449963-81449982 None:intergenic 75.0%
! GCGGCTCCGGTGAGGGCGAG+GGG + chr3.1:81449862-81449881 None:intergenic 80.0%
! GGCGGCTCCGGTGAGGGCGA+GGG + chr3.1:81449863-81449882 None:intergenic 80.0%
! CGGCGGCTCCGGTGAGGGCG+AGG + chr3.1:81449864-81449883 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 81449840 81451455 81449840 ID=MS.gene32890
chr3.1 mRNA 81449840 81451455 81449840 ID=MS.gene32890.t1;Parent=MS.gene32890
chr3.1 exon 81450901 81451455 81450901 ID=MS.gene32890.t1.exon1;Parent=MS.gene32890.t1
chr3.1 CDS 81450901 81451455 81450901 ID=cds.MS.gene32890.t1;Parent=MS.gene32890.t1
chr3.1 exon 81449840 81450622 81449840 ID=MS.gene32890.t1.exon2;Parent=MS.gene32890.t1
chr3.1 CDS 81449840 81450622 81449840 ID=cds.MS.gene32890.t1;Parent=MS.gene32890.t1
Gene Sequence

>MS.gene32890

ATGGAACGCCATTCTCCATCCCCTCGCCCTCACCGGAGCCGCCGCAGCATCTCTCCTCTGCCACGAGACAAGCAACGTCATCATTCTCCTCCACCTCCTTCTTACAACCGCTCTCGTTCTCCTGTGCCACGGGACCAGCCGCAACAACATTCTCATTCTCTAACTAATAGAAGAGATATGAACCATGACCTAATCAAGGTTTCCATGACCATGGCCAACCATTTGCTCTCCAAACAAGAACTTGAAGAAAAGAATGTTGTGTTTTCGCCATTGTTAATCCACTCCGTGCTCAGCATCATCGCGGCTGGCTCTGAGGGTCCCACACAGCAAAAGCTTCTCGACTTCCTTGGCTCAAAATCCATCGATCATCTCAACTCCTTTGCCTCTCATCTCCTTTCAGCCGTACTCAATGATCCTTCTCCTGCCAGTGGGCCTTGCGTCTCTTTTGTCAATGGGCTATGGGTTGAACAATCCCTTTCTCTTCAACCTTCCTTCAAACAAATCGTCTCTACTGATTATAAGGCCACTTTGTCTTCCGTAGATTTCAAGAACAAGGTATTTTTCATTTTCACTGCAATCCATGCTTTATTTTCTGTTAGTTAATGGACCTACCTTCACAATCAATTAGTGTACTTTATTTCATAACATGGTTTAGAGGACATGTTTTACAATTGAAGCATATAATTCAGAGGACATGTTTTACAATTGAAGCATAAATAACCTTTAAGTCGTTCTTTACTAACAAGTATTGCTTTTATTTTTATCTATTTGCATAAGTTATTTATAAATTTGATGATGCATACTTTATTTTAAAATTTGGTATGATTTTGTAGGCTGATAAAGTGACGAATGATGTGAATTCATGGGCCAATAAGGAGACAAATGGCCTTATTGAGAAATTTCTTCCTCCAGGGTTAGTCAACAAATTCACAAAACTCATCTTTGCTAATGCACTGTACTTCAAAGGGGTGTGGAATCAACAATTTGATGCTTCGGAAACAAAAGACTATGATTTTCACATTCATAATGGAAGCTCAGTCAAGGTTCCCTTCATGACCAATGGAGAGAAGCAGTTTATTGGAGCTTTTGATGATTTTAAAGTTCTCCGTCTTCCTTATAACAAAGGTGAAGATACGCGGAAATTCTCTATGTACATTTTTCTTCCAAATCGAAGAGATGGACTGTCAGCTTTGGTTGAGAAGGTGGCTGCTGAATCTGAGTTACTACACCACAAGCTTCGTCTTTCTAAAGTCAAAGTAGGTGATTTCAGAATTCCAAGATTCAAGGTTTCATTTGAACAAGAAATTTCTGATCTCCTGAAGGAGTTAGGAGTGGGTTTAGCTTTCTCTTTGACTAAAATGGTGGACTCTCTTCCAGATCAAGATCAAGACCATTTTGTTTCCCAGATATTTCACAAGTCTTTCATCGAAGTGAATGAAGAAGGAACTGAAGCTGCTGCGGTCACTGCTAATACTGTTGAAATCAAGAGCTTTAGTATAAGTCCTCGACTAGACTTTGTAGCTGACCACCCTTTCTTATTTCTAATTAGAGAAGATTTGACCGAAACGATCCTTTTTGTTGGACATGTGCTCAATCCTCTTGTTAAGTTTTCTTAG
Protein sequence

>MS.gene32890.t1

MERHSPSPRPHRSRRSISPLPRDKQRHHSPPPPSYNRSRSPVPRDQPQQHSHSLTNRRDMNHDLIKVSMTMANHLLSKQELEEKNVVFSPLLIHSVLSIIAAGSEGPTQQKLLDFLGSKSIDHLNSFASHLLSAVLNDPSPASGPCVSFVNGLWVEQSLSLQPSFKQIVSTDYKATLSSVDFKNKADKVTNDVNSWANKETNGLIEKFLPPGLVNKFTKLIFANALYFKGVWNQQFDASETKDYDFHIHNGSSVKVPFMTNGEKQFIGAFDDFKVLRLPYNKGEDTRKFSMYIFLPNRRDGLSALVEKVAAESELLHHKLRLSKVKVGDFRIPRFKVSFEQEISDLLKELGVGLAFSLTKMVDSLPDQDQDHFVSQIFHKSFIEVNEEGTEAAAVTANTVEIKSFSISPRLDFVADHPFLFLIREDLTETILFVGHVLNPLVKFS