Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32921.t1 | XP_013461718.1 | 92.6 | 108 | 8 | 0 | 1 | 108 | 1 | 108 | 1.90E-41 | 178.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32921.t1 | A0A072V157 | 92.6 | 108 | 8 | 0 | 1 | 108 | 1 | 108 | 1.3e-41 | 178.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061114 | MS.gene32921 | -0.800968 | 1.12E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32921.t1 | MTR_3g102270 | 94.624 | 93 | 5 | 0 | 16 | 108 | 16 | 108 | 6.44e-63 | 189 |
| MS.gene32921.t1 | MTR_3g102270 | 94.624 | 93 | 5 | 0 | 16 | 108 | 16 | 108 | 1.38e-62 | 189 |
| MS.gene32921.t1 | MTR_3g102270 | 94.624 | 93 | 5 | 0 | 16 | 108 | 16 | 108 | 9.45e-62 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGATTTAACGTGTTGTTAA+CGG | 0.274705 | 3.1:-81242148 | None:intergenic |
| AAGAATCTGATTATCGAAAA+TGG | 0.276535 | 3.1:+81242211 | MS.gene32921:CDS |
| TTGCGGCGCGCCATCGTTCA+TGG | 0.324166 | 3.1:+81242070 | MS.gene32921:CDS |
| TCCTTAGTAAGCACGATAAT+AGG | 0.329251 | 3.1:-81242270 | None:intergenic |
| CGGTGAAGTGTTTGTAGTTA+TGG | 0.331589 | 3.1:-81242038 | None:intergenic |
| TGGCCGCACCACTTCTTCTA+AGG | 0.356825 | 3.1:+81241989 | MS.gene32921:CDS |
| ACCTATTATCGTGCTTACTA+AGG | 0.426424 | 3.1:+81242269 | MS.gene32921:CDS |
| AACGTGTTGTTAACGGAGTT+AGG | 0.441542 | 3.1:-81242141 | None:intergenic |
| ACGGAGTTAGGTTTATCGTT+TGG | 0.446191 | 3.1:-81242129 | None:intergenic |
| CCGTTAACAACACGTTAAAT+CGG | 0.462228 | 3.1:+81242148 | MS.gene32921:CDS |
| CCCGTCGAGTAACTTCCGTT+TGG | 0.471763 | 3.1:+81242182 | MS.gene32921:CDS |
| CAGATTCTTCATCTTCCAAA+CGG | 0.510640 | 3.1:-81242197 | None:intergenic |
| CGGCGCTGCACCATGAACGA+TGG | 0.551636 | 3.1:-81242080 | None:intergenic |
| AGAGCTTGCCTTAGAAGAAG+TGG | 0.555098 | 3.1:-81241997 | None:intergenic |
| TTCCGACGGTGGACTGATGG+CGG | 0.556393 | 3.1:-81242103 | None:intergenic |
| TGAACGATGGCGCGCCGCAA+CGG | 0.567387 | 3.1:-81242067 | None:intergenic |
| CGCCGCCATCAGTCCACCGT+CGG | 0.579096 | 3.1:+81242101 | MS.gene32921:CDS |
| GCGCGCCGCAACGGTTCTGA+CGG | 0.584424 | 3.1:-81242058 | None:intergenic |
| CCAAACGGAAGTTACTCGAC+GGG | 0.593161 | 3.1:-81242182 | None:intergenic |
| ATCTGATTATCGAAAATGGA+AGG | 0.596386 | 3.1:+81242215 | MS.gene32921:CDS |
| CGACGGTGGACTGATGGCGG+CGG | 0.597651 | 3.1:-81242100 | None:intergenic |
| TCCAAACGGAAGTTACTCGA+CGG | 0.641479 | 3.1:-81242183 | None:intergenic |
| TTGCCTTAGAAGAAGTGGTG+CGG | 0.691027 | 3.1:-81241992 | None:intergenic |
| CTTCACCGTCAGAACCGTTG+CGG | 0.698015 | 3.1:+81242053 | MS.gene32921:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAATCTGATTATCGAAAA+TGG | + | chr3.1:81242211-81242230 | MS.gene32921:CDS | 25.0% |
| ATCTGATTATCGAAAATGGA+AGG | + | chr3.1:81242215-81242234 | MS.gene32921:CDS | 30.0% | |
| ACCTATTATCGTGCTTACTA+AGG | + | chr3.1:81242269-81242288 | MS.gene32921:CDS | 35.0% | |
| CAGATTCTTCATCTTCCAAA+CGG | - | chr3.1:81242200-81242219 | None:intergenic | 35.0% | |
| CCGTTAACAACACGTTAAAT+CGG | + | chr3.1:81242148-81242167 | MS.gene32921:CDS | 35.0% | |
| TCCTTAGTAAGCACGATAAT+AGG | - | chr3.1:81242273-81242292 | None:intergenic | 35.0% | |
| ! | CCGATTTAACGTGTTGTTAA+CGG | - | chr3.1:81242151-81242170 | None:intergenic | 35.0% |
| !!! | TTATCGTTTGGTTTTTCCGA+CGG | - | chr3.1:81242120-81242139 | None:intergenic | 35.0% |
| ACGGAGTTAGGTTTATCGTT+TGG | - | chr3.1:81242132-81242151 | None:intergenic | 40.0% | |
| ! | AACGTGTTGTTAACGGAGTT+AGG | - | chr3.1:81242144-81242163 | None:intergenic | 40.0% |
| ! | CGGTGAAGTGTTTGTAGTTA+TGG | - | chr3.1:81242041-81242060 | None:intergenic | 40.0% |
| AGAGCTTGCCTTAGAAGAAG+TGG | - | chr3.1:81242000-81242019 | None:intergenic | 45.0% | |
| TCCAAACGGAAGTTACTCGA+CGG | - | chr3.1:81242186-81242205 | None:intergenic | 45.0% | |
| TTGCCTTAGAAGAAGTGGTG+CGG | - | chr3.1:81241995-81242014 | None:intergenic | 45.0% | |
| CCAAACGGAAGTTACTCGAC+GGG | - | chr3.1:81242185-81242204 | None:intergenic | 50.0% | |
| !!! | TCGTTTGGTTTTTCCGACGG+TGG | - | chr3.1:81242117-81242136 | None:intergenic | 50.0% |
| !!! | TTTTTCCGACGGTGGACTGA+TGG | - | chr3.1:81242109-81242128 | None:intergenic | 50.0% |
| CCCGTCGAGTAACTTCCGTT+TGG | + | chr3.1:81242182-81242201 | MS.gene32921:CDS | 55.0% | |
| CTTCACCGTCAGAACCGTTG+CGG | + | chr3.1:81242053-81242072 | MS.gene32921:CDS | 55.0% | |
| TGGCCGCACCACTTCTTCTA+AGG | + | chr3.1:81241989-81242008 | MS.gene32921:CDS | 55.0% | |
| !! | TTCCGACGGTGGACTGATGG+CGG | - | chr3.1:81242106-81242125 | None:intergenic | 60.0% |
| CGGCGCTGCACCATGAACGA+TGG | - | chr3.1:81242083-81242102 | None:intergenic | 65.0% | |
| TGAACGATGGCGCGCCGCAA+CGG | - | chr3.1:81242070-81242089 | None:intergenic | 65.0% | |
| TTGCGGCGCGCCATCGTTCA+TGG | + | chr3.1:81242070-81242089 | MS.gene32921:CDS | 65.0% | |
| CGCCGCCATCAGTCCACCGT+CGG | + | chr3.1:81242101-81242120 | MS.gene32921:CDS | 70.0% | |
| ! | GCGCGCCGCAACGGTTCTGA+CGG | - | chr3.1:81242061-81242080 | None:intergenic | 70.0% |
| !! | CGACGGTGGACTGATGGCGG+CGG | - | chr3.1:81242103-81242122 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 81241988 | 81242314 | 81241988 | ID=MS.gene32921 |
| chr3.1 | mRNA | 81241988 | 81242314 | 81241988 | ID=MS.gene32921.t1;Parent=MS.gene32921 |
| chr3.1 | exon | 81241988 | 81242314 | 81241988 | ID=MS.gene32921.t1.exon1;Parent=MS.gene32921.t1 |
| chr3.1 | CDS | 81241988 | 81242314 | 81241988 | ID=cds.MS.gene32921.t1;Parent=MS.gene32921.t1 |
| Gene Sequence |
| Protein sequence |
