Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32923.t1 | AFK37330.1 | 73.7 | 114 | 14 | 1 | 1 | 114 | 1 | 98 | 6.60E-37 | 163.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32923.t1 | I3SAN8 | 73.7 | 114 | 14 | 1 | 1 | 114 | 1 | 98 | 4.7e-37 | 163.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene32923 | 0.817194 | 3.74E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene32923 | 0.804412 | 2.19E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene32923 | 0.818024 | 2.43E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene32923 | 0.830946 | 2.21E-55 | -1.69E-46 |
| MS.gene054930 | MS.gene32923 | 0.805872 | 1.08E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene32923 | 0.824631 | 7.27E-54 | -1.69E-46 |
| MS.gene056723 | MS.gene32923 | 0.809466 | 1.87E-50 | -1.69E-46 |
| MS.gene057121 | MS.gene32923 | 0.810273 | 1.25E-50 | -1.69E-46 |
| MS.gene057469 | MS.gene32923 | 0.818466 | 1.93E-52 | -1.69E-46 |
| MS.gene058142 | MS.gene32923 | 0.816453 | 5.49E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32923.t1 | MTR_3g102270 | 56.000 | 100 | 39 | 3 | 16 | 113 | 16 | 112 | 4.73e-30 | 105 |
| MS.gene32923.t1 | MTR_3g102270 | 56.000 | 100 | 39 | 3 | 16 | 113 | 16 | 112 | 1.02e-29 | 105 |
| MS.gene32923.t1 | MTR_3g102270 | 56.000 | 100 | 39 | 3 | 16 | 113 | 16 | 112 | 2.44e-29 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGATTTAACGTGTTGTTAA+CGG | 0.274705 | 3.1:-81083648 | None:intergenic |
| AAGAATCTGATTATCGAAAA+TGG | 0.276535 | 3.1:+81083710 | MS.gene32923:CDS |
| TTGCGGCGCGCCATCGTTCA+TGG | 0.324166 | 3.1:+81083570 | MS.gene32923:CDS |
| TCCTTAGTAAGCACGATAAT+AGG | 0.329251 | 3.1:-81083769 | None:intergenic |
| CGGTGAAGTGTTTGTAGTTA+TGG | 0.331589 | 3.1:-81083538 | None:intergenic |
| TGGCCGCACCACTTCTTCTA+AGG | 0.356825 | 3.1:+81083489 | MS.gene32923:CDS |
| ACCTATTATCGTGCTTACTA+AGG | 0.426424 | 3.1:+81083768 | MS.gene32923:CDS |
| AACGTGTTGTTAACGGAGTT+AGG | 0.441542 | 3.1:-81083641 | None:intergenic |
| ACGGAGTTAGGTTTATCGTT+TGG | 0.446191 | 3.1:-81083629 | None:intergenic |
| CCGTTAACAACACGTTAAAT+CGG | 0.462228 | 3.1:+81083648 | MS.gene32923:CDS |
| CAGATTCTTCATCTTCCAAA+CGG | 0.510640 | 3.1:-81083696 | None:intergenic |
| ACCGTCGAGTAACTTCCGTT+TGG | 0.520429 | 3.1:+81083681 | MS.gene32923:CDS |
| CGGCGCTGCACCATGAACGA+TGG | 0.551636 | 3.1:-81083580 | None:intergenic |
| AGAGCTTGCCTTAGAAGAAG+TGG | 0.555098 | 3.1:-81083497 | None:intergenic |
| TTCCGACGGTGGACTGATGG+CGG | 0.556393 | 3.1:-81083603 | None:intergenic |
| TGAACGATGGCGCGCCGCAA+CGG | 0.567387 | 3.1:-81083567 | None:intergenic |
| CGCCGCCATCAGTCCACCGT+CGG | 0.579096 | 3.1:+81083601 | MS.gene32923:CDS |
| GCGCGCCGCAACGGTTCTGA+CGG | 0.584424 | 3.1:-81083558 | None:intergenic |
| ATCTGATTATCGAAAATGGA+AGG | 0.596386 | 3.1:+81083714 | MS.gene32923:CDS |
| CGACGGTGGACTGATGGCGG+CGG | 0.597651 | 3.1:-81083600 | None:intergenic |
| TTGTGTGCTAGGTATATGGA+TGG | 0.625552 | 3.1:+81083879 | MS.gene32923:intron |
| TGTGCTAGGTATATGGATGG+AGG | 0.667189 | 3.1:+81083882 | MS.gene32923:intron |
| GTGCTAGGTATATGGATGGA+GGG | 0.681449 | 3.1:+81083883 | MS.gene32923:intron |
| TCCAAACGGAAGTTACTCGA+CGG | 0.689176 | 3.1:-81083682 | None:intergenic |
| TTGCCTTAGAAGAAGTGGTG+CGG | 0.691027 | 3.1:-81083492 | None:intergenic |
| CTTCACCGTCAGAACCGTTG+CGG | 0.698015 | 3.1:+81083553 | MS.gene32923:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAATCTGATTATCGAAAA+TGG | + | chr3.1:81083710-81083729 | MS.gene32923:CDS | 25.0% |
| !!! | TGAATTTAATTTTGTGTGCT+AGG | + | chr3.1:81083868-81083887 | MS.gene32923:intron | 25.0% |
| ATCTGATTATCGAAAATGGA+AGG | + | chr3.1:81083714-81083733 | MS.gene32923:CDS | 30.0% | |
| ! | AATTTTGTGTGCTAGGTATA+TGG | + | chr3.1:81083875-81083894 | MS.gene32923:intron | 30.0% |
| ACCTATTATCGTGCTTACTA+AGG | + | chr3.1:81083768-81083787 | MS.gene32923:CDS | 35.0% | |
| CAGATTCTTCATCTTCCAAA+CGG | - | chr3.1:81083699-81083718 | None:intergenic | 35.0% | |
| CCGTTAACAACACGTTAAAT+CGG | + | chr3.1:81083648-81083667 | MS.gene32923:CDS | 35.0% | |
| TCCTTAGTAAGCACGATAAT+AGG | - | chr3.1:81083772-81083791 | None:intergenic | 35.0% | |
| ! | CCGATTTAACGTGTTGTTAA+CGG | - | chr3.1:81083651-81083670 | None:intergenic | 35.0% |
| !!! | TTATCGTTTGGTTTTTCCGA+CGG | - | chr3.1:81083620-81083639 | None:intergenic | 35.0% |
| ACGGAGTTAGGTTTATCGTT+TGG | - | chr3.1:81083632-81083651 | None:intergenic | 40.0% | |
| TTGTGTGCTAGGTATATGGA+TGG | + | chr3.1:81083879-81083898 | MS.gene32923:intron | 40.0% | |
| ! | AACGTGTTGTTAACGGAGTT+AGG | - | chr3.1:81083644-81083663 | None:intergenic | 40.0% |
| ! | CGGTGAAGTGTTTGTAGTTA+TGG | - | chr3.1:81083541-81083560 | None:intergenic | 40.0% |
| AGAGCTTGCCTTAGAAGAAG+TGG | - | chr3.1:81083500-81083519 | None:intergenic | 45.0% | |
| GTGCTAGGTATATGGATGGA+GGG | + | chr3.1:81083883-81083902 | MS.gene32923:intron | 45.0% | |
| TCCAAACGGAAGTTACTCGA+CGG | - | chr3.1:81083685-81083704 | None:intergenic | 45.0% | |
| TGTGCTAGGTATATGGATGG+AGG | + | chr3.1:81083882-81083901 | MS.gene32923:intron | 45.0% | |
| TTGCCTTAGAAGAAGTGGTG+CGG | - | chr3.1:81083495-81083514 | None:intergenic | 45.0% | |
| ACCGTCGAGTAACTTCCGTT+TGG | + | chr3.1:81083681-81083700 | MS.gene32923:CDS | 50.0% | |
| !!! | TCGTTTGGTTTTTCCGACGG+TGG | - | chr3.1:81083617-81083636 | None:intergenic | 50.0% |
| !!! | TTTTTCCGACGGTGGACTGA+TGG | - | chr3.1:81083609-81083628 | None:intergenic | 50.0% |
| CTTCACCGTCAGAACCGTTG+CGG | + | chr3.1:81083553-81083572 | MS.gene32923:CDS | 55.0% | |
| TGGCCGCACCACTTCTTCTA+AGG | + | chr3.1:81083489-81083508 | MS.gene32923:CDS | 55.0% | |
| !! | TTCCGACGGTGGACTGATGG+CGG | - | chr3.1:81083606-81083625 | None:intergenic | 60.0% |
| CGGCGCTGCACCATGAACGA+TGG | - | chr3.1:81083583-81083602 | None:intergenic | 65.0% | |
| TGAACGATGGCGCGCCGCAA+CGG | - | chr3.1:81083570-81083589 | None:intergenic | 65.0% | |
| TTGCGGCGCGCCATCGTTCA+TGG | + | chr3.1:81083570-81083589 | MS.gene32923:CDS | 65.0% | |
| CGCCGCCATCAGTCCACCGT+CGG | + | chr3.1:81083601-81083620 | MS.gene32923:CDS | 70.0% | |
| ! | GCGCGCCGCAACGGTTCTGA+CGG | - | chr3.1:81083561-81083580 | None:intergenic | 70.0% |
| !! | CGACGGTGGACTGATGGCGG+CGG | - | chr3.1:81083603-81083622 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 81083488 | 81083912 | 81083488 | ID=MS.gene32923 |
| chr3.1 | mRNA | 81083488 | 81083912 | 81083488 | ID=MS.gene32923.t1;Parent=MS.gene32923 |
| chr3.1 | exon | 81083488 | 81083809 | 81083488 | ID=MS.gene32923.t1.exon1;Parent=MS.gene32923.t1 |
| chr3.1 | CDS | 81083488 | 81083809 | 81083488 | ID=cds.MS.gene32923.t1;Parent=MS.gene32923.t1 |
| chr3.1 | exon | 81083890 | 81083912 | 81083890 | ID=MS.gene32923.t1.exon2;Parent=MS.gene32923.t1 |
| chr3.1 | CDS | 81083890 | 81083912 | 81083890 | ID=cds.MS.gene32923.t1;Parent=MS.gene32923.t1 |
| Gene Sequence |
| Protein sequence |
