AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33012

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33012.t1 MTR_1g016080 93.640 283 14 1 1 283 2 280 1.68e-178 493
MS.gene33012.t1 MTR_3g104420 65.918 267 88 2 19 283 7 272 8.13e-130 369
MS.gene33012.t1 MTR_2g006770 43.404 235 133 0 41 275 17 251 1.20e-66 209
MS.gene33012.t1 MTR_8g089715 40.517 232 135 1 42 270 15 246 2.21e-63 200
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33012.t1 AT5G11870 51.825 274 125 4 11 282 1 269 6.22e-101 296
MS.gene33012.t1 AT5G11870 51.685 267 122 4 11 275 1 262 1.78e-97 287
MS.gene33012.t1 AT1G71190 41.245 257 150 1 25 280 1 257 1.27e-61 196
MS.gene33012.t1 AT1G71190 35.366 246 119 1 35 280 11 216 1.42e-48 161

Find 54 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAGGAGAGCGTGGAATTT+TGG 0.177744 1.1:-6050534 None:intergenic
CTATTCTATGCTGGAATTAT+AGG 0.182046 1.1:+6050885 MS.gene33012:CDS
TATTCTATGCTGGAATTATA+GGG 0.258418 1.1:+6050886 MS.gene33012:CDS
CGTTATCAGCGGGCACTCTT+TGG 0.268066 1.1:+6052323 MS.gene33012:CDS
GCTGACTTGCGCAATCTTCT+TGG 0.276021 1.1:+6050569 MS.gene33012:CDS
GGAATTATAGGGGTAGCTTT+TGG 0.299826 1.1:+6050897 MS.gene33012:CDS
ACTATACTCACTCAAGATAT+TGG 0.300455 1.1:+6052150 MS.gene33012:CDS
ACAGGGAGCGTAAGTTTAAA+AGG 0.336107 1.1:+6052397 MS.gene33012:CDS
GTGATCACATTCACCGTGTT+GGG 0.344613 1.1:-6050683 None:intergenic
TTCAGAATTGGAGTTTGAAT+TGG 0.355442 1.1:+6050399 MS.gene33012:CDS
GCAGAACTTATGATGATATT+CGG 0.357442 1.1:+6052060 MS.gene33012:intron
GGTTTAGTTCTTACTCTTGA+AGG 0.365060 1.1:+6050737 MS.gene33012:CDS
AGAGAATTGGCCAGCGGATT+GGG 0.369422 1.1:+6051860 MS.gene33012:CDS
AGTCTTGTGGGACACTTTGC+CGG 0.373549 1.1:+6050956 MS.gene33012:CDS
CACAAGACTCTATCGTTATC+AGG 0.387981 1.1:-6050942 None:intergenic
AGTTTGGTTATTGAGAGAAT+TGG 0.409279 1.1:+6051847 MS.gene33012:CDS
TGACGATGATGATTCAGAAT+TGG 0.423301 1.1:+6050387 None:intergenic
TTAGTGTGATGCTCATCTAC+AGG 0.457946 1.1:+6052379 MS.gene33012:CDS
GAGAGAATTGGCCAGCGGAT+TGG 0.461721 1.1:+6051859 MS.gene33012:CDS
TAGCTAAAGGAAGAATGAAC+TGG 0.473251 1.1:-6052097 None:intergenic
TTAGTTCTCAAGCAGAGATA+TGG 0.474235 1.1:+6050853 MS.gene33012:intron
TTCATAAACAACACAAGTAA+AGG 0.483567 1.1:-6051914 None:intergenic
TTTGGGAGAGAAGAGAAAGA+AGG 0.500392 1.1:-6050516 None:intergenic
GATTGCGCAAGTCAGCGAAT+TGG 0.503286 1.1:-6050562 None:intergenic
TTACTTGTGTTGTTTATGAA+CGG 0.506820 1.1:+6051917 MS.gene33012:CDS
TATCGTTATCAGGCTTCAAA+TGG 0.512826 1.1:-6050932 None:intergenic
TGATAACGATAGAGTCTTGT+GGG 0.513805 1.1:+6050944 MS.gene33012:CDS
TGTGATCACATTCACCGTGT+TGG 0.514498 1.1:-6050684 None:intergenic
CTCAAGCAGAGATATGGTCT+TGG 0.517070 1.1:+6050859 MS.gene33012:CDS
CACAAGTAAAGGCTGCGAAA+AGG 0.526322 1.1:-6051903 None:intergenic
TTTGGAACACTTGTGCTTAG+CGG 0.540650 1.1:+6052341 MS.gene33012:CDS
AAGTAAAGGCTGCGAAAAGG+AGG 0.541078 1.1:-6051900 None:intergenic
TGGTGGTGAGTAGGAGAGCG+TGG 0.543307 1.1:-6050542 None:intergenic
TGCGCAAGTCAGCGAATTGG+TGG 0.545924 1.1:-6050559 None:intergenic
GCAGCTGCTGCAATAGCTAA+AGG 0.548300 1.1:-6052110 None:intergenic
TCTTGGATACTATTCTATGC+TGG 0.553325 1.1:+6050876 MS.gene33012:CDS
TCAGCGAATTGGTGGTGAGT+AGG 0.573822 1.1:-6050551 None:intergenic
GCAAATAACTACGTTATCAG+CGG 0.576718 1.1:+6052312 MS.gene33012:CDS
GAATTGGAGTTTGAATTGGA+TGG 0.576928 1.1:+6050403 MS.gene33012:CDS
TGATCACATTCACCGTGTTG+GGG 0.577459 1.1:-6050682 None:intergenic
CAAATAACTACGTTATCAGC+GGG 0.577528 1.1:+6052313 MS.gene33012:CDS
TAGTGTGATGCTCATCTACA+GGG 0.584946 1.1:+6052380 MS.gene33012:CDS
TACAACACATCCCAATCCGC+TGG 0.586145 1.1:-6051870 None:intergenic
TTAGTTCTTACTCTTGAAGG+AGG 0.606045 1.1:+6050740 MS.gene33012:CDS
CTGATAACGATAGAGTCTTG+TGG 0.611073 1.1:+6050943 MS.gene33012:CDS
CTTGAGTGAGTATAGTCAGA+GGG 0.627291 1.1:-6052143 None:intergenic
AGTAAAGGCTGCGAAAAGGA+GGG 0.633786 1.1:-6051899 None:intergenic
ATTCTATGCTGGAATTATAG+GGG 0.637655 1.1:+6050887 MS.gene33012:CDS
TGATAACGTAGTTATTTGCA+CGG 0.644610 1.1:-6052308 None:intergenic
TCTTGAGTGAGTATAGTCAG+AGG 0.667258 1.1:-6052144 None:intergenic
TTTCACAGGTGTCCCCAACA+CGG 0.676395 1.1:+6050670 MS.gene33012:intron
GCACTAATGGCAGATGATGG+TGG 0.681415 1.1:+6051804 MS.gene33012:intron
AACCAAACTAGAGAAAAGTG+AGG 0.710937 1.1:-6051833 None:intergenic
TTATTGAGAGAATTGGCCAG+CGG 0.722249 1.1:+6051854 MS.gene33012:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATATATTCATATAGAG+AGG - chr1.1:6051005-6051024 None:intergenic 15.0%
!!! AACTATTTCTATATTTGAAT+AGG + chr1.1:6051404-6051423 MS.gene33012:intron 15.0%
!!! ACTATTTCTATATTTGAATA+GGG + chr1.1:6051405-6051424 MS.gene33012:intron 15.0%
!!! AGATTTCATTTTTCTTAATT+TGG + chr1.1:6050627-6050646 MS.gene33012:intron 15.0%
!!! TTTAAATGTTTTATACTTTG+AGG + chr1.1:6051986-6052005 MS.gene33012:intron 15.0%
!! AAACTAAAATAAGTCAATCA+GGG - chr1.1:6052831-6052850 None:intergenic 20.0%
!! AATAAGCTAAAAATAGCTTA+TGG + chr1.1:6051285-6051304 MS.gene33012:intron 20.0%
!! AGGAAACATTAATTACAAAA+AGG - chr1.1:6053123-6053142 None:intergenic 20.0%
!! CTTATAAAGAGAAAATGTTA+AGG + chr1.1:6051673-6051692 MS.gene33012:intron 20.0%
!! TAAACTAAAATAAGTCAATC+AGG - chr1.1:6052832-6052851 None:intergenic 20.0%
!! TCTGTCATAATTTAAACAAA+TGG - chr1.1:6051645-6051664 None:intergenic 20.0%
!!! ATGACATTTCAAATTTTTGA+AGG - chr1.1:6052574-6052593 None:intergenic 20.0%
!!! ATTGTTTTCATAAGATATCT+TGG - chr1.1:6052957-6052976 None:intergenic 20.0%
!!! CTATTTCTATATTTGAATAG+GGG + chr1.1:6051406-6051425 MS.gene33012:intron 20.0%
!!! TAAGCTATTTCAATAATGTA+AGG + chr1.1:6051150-6051169 MS.gene33012:intron 20.0%
!!! TAATTTTAGCATAAACTTAG+TGG - chr1.1:6052023-6052042 None:intergenic 20.0%
!!! TTCATTTTTCTTAATTTGGA+TGG + chr1.1:6050631-6050650 MS.gene33012:intron 20.0%
!!! TTTTGTAATTAATGTTTCCT+TGG + chr1.1:6053123-6053142 MS.gene33012:intron 20.0%
! AACTAAAATAAGTCAATCAG+GGG - chr1.1:6052830-6052849 None:intergenic 25.0%
! ATAAAGAGAAAATGTTAAGG+TGG + chr1.1:6051676-6051695 MS.gene33012:intron 25.0%
! ATATATTCATATAGAGAGGT+AGG - chr1.1:6051001-6051020 None:intergenic 25.0%
! GATAAAAGTAACTAACCTTA+CGG - chr1.1:6050774-6050793 None:intergenic 25.0%
! TAAAATTATGATCTGCTAGT+TGG + chr1.1:6052035-6052054 MS.gene33012:intron 25.0%
! TATTCTATGCTGGAATTATA+GGG + chr1.1:6050886-6050905 MS.gene33012:CDS 25.0%
! TGTGATTAGATTCTAATATG+AGG + chr1.1:6050814-6050833 MS.gene33012:intron 25.0%
! TTCATAAACAACACAAGTAA+AGG - chr1.1:6051917-6051936 None:intergenic 25.0%
!! TTAAGTTGTTTATCCAAACT+AGG - chr1.1:6051176-6051195 None:intergenic 25.0%
!! TTCTGAAGAAAAAAACTAGT+GGG - chr1.1:6052597-6052616 None:intergenic 25.0%
!! TTTTCATAAGATATCTTGGA+GGG - chr1.1:6052953-6052972 None:intergenic 25.0%
!!! AAATTTCCCATTTTTAGTTG+TGG + chr1.1:6050706-6050725 MS.gene33012:CDS 25.0%
!!! AATTTCCCATTTTTAGTTGT+GGG + chr1.1:6050707-6050726 MS.gene33012:CDS 25.0%
!!! AGATATTGGTTTTCATCAAT+AGG + chr1.1:6052164-6052183 MS.gene33012:CDS 25.0%
!!! TTACTTGTGTTGTTTATGAA+CGG + chr1.1:6051917-6051936 MS.gene33012:CDS 25.0%
!!! TTTCTTAATTTGGATGGTTT+TGG + chr1.1:6050637-6050656 MS.gene33012:intron 25.0%
AAAACGAACTAAGTACTAAG+AGG - chr1.1:6051756-6051775 None:intergenic 30.0%
AACCAAAAATTCCATCGAAT+AGG - chr1.1:6052437-6052456 None:intergenic 30.0%
AAGCTTGAGACTATTATTAG+AGG - chr1.1:6051957-6051976 None:intergenic 30.0%
ACAAAAGATAAACCAAAGTC+AGG + chr1.1:6051241-6051260 MS.gene33012:intron 30.0%
ACCAATGAGCTTCTTAATAA+AGG - chr1.1:6051783-6051802 None:intergenic 30.0%
ACTATACTCACTCAAGATAT+TGG + chr1.1:6052150-6052169 MS.gene33012:CDS 30.0%
AGCATCACACTAAGTAAAAA+CGG - chr1.1:6052372-6052391 None:intergenic 30.0%
AGTTTGGTTATTGAGAGAAT+TGG + chr1.1:6051847-6051866 MS.gene33012:CDS 30.0%
ATAAATGTTGCAAAAGTCTG+AGG - chr1.1:6052197-6052216 None:intergenic 30.0%
ATGCTATAAGCACTTAATTG+TGG + chr1.1:6053064-6053083 MS.gene33012:intron 30.0%
ATTATAGAAGAGAGCTTATG+TGG - chr1.1:6051445-6051464 None:intergenic 30.0%
ATTCTATGCTGGAATTATAG+GGG + chr1.1:6050887-6050906 MS.gene33012:CDS 30.0%
CAATGAGCTTCTTAATAAAG+GGG - chr1.1:6051781-6051800 None:intergenic 30.0%
CCAATGAGCTTCTTAATAAA+GGG - chr1.1:6051782-6051801 None:intergenic 30.0%
CCTATTCAAAAACCAATTCA+TGG - chr1.1:6052724-6052743 None:intergenic 30.0%
CTATTCTATGCTGGAATTAT+AGG + chr1.1:6050885-6050904 MS.gene33012:CDS 30.0%
GCAGAACTTATGATGATATT+CGG + chr1.1:6052060-6052079 MS.gene33012:intron 30.0%
TTAAACAAATGGAGCAACTA+GGG - chr1.1:6051634-6051653 None:intergenic 30.0%
TTCAGAATTGGAGTTTGAAT+TGG + chr1.1:6050399-6050418 MS.gene33012:CDS 30.0%
TTCTTTAGCCAAAACAATCA+TGG - chr1.1:6051072-6051091 None:intergenic 30.0%
TTGTGAAACCAAATTTCACA+AGG - chr1.1:6052460-6052479 None:intergenic 30.0%
TTTAAACAAATGGAGCAACT+AGG - chr1.1:6051635-6051654 None:intergenic 30.0%
! AACCTATTCGATGGAATTTT+TGG + chr1.1:6052432-6052451 MS.gene33012:intron 30.0%
! ATTTTTACCTCACCATGAAT+TGG + chr1.1:6052709-6052728 MS.gene33012:intron 30.0%
! CTTCTGAAGAAAAAAACTAG+TGG - chr1.1:6052598-6052617 None:intergenic 30.0%
! GTTTTCATAAGATATCTTGG+AGG - chr1.1:6052954-6052973 None:intergenic 30.0%
! TGATAACGTAGTTATTTGCA+CGG - chr1.1:6052311-6052330 None:intergenic 30.0%
! TTCCTTTTCCATGATTGTTT+TGG + chr1.1:6051061-6051080 MS.gene33012:intron 30.0%
! TTTGGTTTCACAAATTGTTC+TGG + chr1.1:6052467-6052486 MS.gene33012:intron 30.0%
!! TATGAGGTGTGTTTTTGATT+TGG + chr1.1:6050830-6050849 MS.gene33012:intron 30.0%
!!! AGTGTTTTAAAGGGCTTATT+TGG - chr1.1:6051611-6051630 None:intergenic 30.0%
!!! CCATGAATTGGTTTTTGAAT+AGG + chr1.1:6052721-6052740 MS.gene33012:intron 30.0%
!!! TTTTGGTTCCTTGTGAAATT+TGG + chr1.1:6052449-6052468 MS.gene33012:intron 30.0%
AACCAAACTAGAGAAAAGTG+AGG - chr1.1:6051836-6051855 None:intergenic 35.0%
AGAACACCCACAACTAAAAA+TGG - chr1.1:6050716-6050735 None:intergenic 35.0%
AGCCAAAACAATCATGGAAA+AGG - chr1.1:6051066-6051085 None:intergenic 35.0%
AGCCTAGACAAACTAGAATT+TGG - chr1.1:6052627-6052646 None:intergenic 35.0%
CAAATAACTACGTTATCAGC+GGG + chr1.1:6052313-6052332 MS.gene33012:CDS 35.0%
CAATAACACTTGTGTGAGTT+TGG - chr1.1:6051572-6051591 None:intergenic 35.0%
CAATAATGTAAGGCCTAGTT+TGG + chr1.1:6051160-6051179 MS.gene33012:intron 35.0%
CATATAGAGAGGTAGGTAAT+GGG - chr1.1:6050994-6051013 None:intergenic 35.0%
CCCTTTATTAAGAAGCTCAT+TGG + chr1.1:6051779-6051798 MS.gene33012:intron 35.0%
CTATATTTGAATAGGGGATG+CGG + chr1.1:6051412-6051431 MS.gene33012:intron 35.0%
GAACACCCACAACTAAAAAT+GGG - chr1.1:6050715-6050734 None:intergenic 35.0%
GAATTGGAGTTTGAATTGGA+TGG + chr1.1:6050403-6050422 MS.gene33012:CDS 35.0%
GCAAATAACTACGTTATCAG+CGG + chr1.1:6052312-6052331 MS.gene33012:CDS 35.0%
GCATCACACTAAGTAAAAAC+GGG - chr1.1:6052371-6052390 None:intergenic 35.0%
GTCTTGTGGATGTTTGAATT+TGG - chr1.1:6052539-6052558 None:intergenic 35.0%
TAGCTAAAGGAAGAATGAAC+TGG - chr1.1:6052100-6052119 None:intergenic 35.0%
TATCGTTATCAGGCTTCAAA+TGG - chr1.1:6050935-6050954 None:intergenic 35.0%
TCAAAAACCAATTCATGGTG+AGG - chr1.1:6052719-6052738 None:intergenic 35.0%
TCATATAGAGAGGTAGGTAA+TGG - chr1.1:6050995-6051014 None:intergenic 35.0%
TCTTGGATACTATTCTATGC+TGG + chr1.1:6050876-6050895 MS.gene33012:CDS 35.0%
! CTATTAGCACGTGTTTGTTT+TGG + chr1.1:6052744-6052763 MS.gene33012:intron 35.0%
! TGATAACGATAGAGTCTTGT+GGG + chr1.1:6050944-6050963 MS.gene33012:CDS 35.0%
! TGATCAAAATCGATTCCTGA+TGG - chr1.1:6052657-6052676 None:intergenic 35.0%
! TTAGTTCTCAAGCAGAGATA+TGG + chr1.1:6050853-6050872 MS.gene33012:intron 35.0%
! TTAGTTCTTACTCTTGAAGG+AGG + chr1.1:6050740-6050759 MS.gene33012:CDS 35.0%
!! GAATTTGGAAAACTGACTTG+AGG - chr1.1:6052524-6052543 None:intergenic 35.0%
!! GGTTTAGTTCTTACTCTTGA+AGG + chr1.1:6050737-6050756 MS.gene33012:CDS 35.0%
!! TATTTGAAGCCTGACCTTTT+TGG + chr1.1:6052231-6052250 MS.gene33012:intron 35.0%
!! TTCCTCACTTTTCTCTAGTT+TGG + chr1.1:6051831-6051850 MS.gene33012:CDS 35.0%
!! TTGATGATGAACCTATTCGA+TGG + chr1.1:6052423-6052442 MS.gene33012:intron 35.0%
!! TTTTTGAAGGACAGATGTTG+TGG - chr1.1:6052561-6052580 None:intergenic 35.0%
!!! AAGGAGGATTTTTTACCGTA+AGG + chr1.1:6050756-6050775 MS.gene33012:CDS 35.0%
!!! ATTTGGATGGTTTTGGTTTC+TGG + chr1.1:6050644-6050663 MS.gene33012:intron 35.0%
!!! CAACTAGGGAGTGTTTTAAA+GGG - chr1.1:6051620-6051639 None:intergenic 35.0%
!!! TTTGGATGGTTTTGGTTTCT+GGG + chr1.1:6050645-6050664 MS.gene33012:intron 35.0%
!!! TTTTTAGTTGTGGGTGTTCT+TGG + chr1.1:6050716-6050735 MS.gene33012:CDS 35.0%
ATACATGCGTAGTGTCTTCT+CGG - chr1.1:6051383-6051402 None:intergenic 40.0%
ATGTTTCCTTGGAAGAGCTT+TGG + chr1.1:6053134-6053153 MS.gene33012:intron 40.0%
ATTGTGGAGAACAACGTTGA+AGG + chr1.1:6053080-6053099 MS.gene33012:intron 40.0%
CACAAGACTCTATCGTTATC+AGG - chr1.1:6050945-6050964 None:intergenic 40.0%
CTTGAGTGAGTATAGTCAGA+GGG - chr1.1:6052146-6052165 None:intergenic 40.0%
TAATGGGAATGCTAACCAAC+CGG - chr1.1:6050978-6050997 None:intergenic 40.0%
TAGTGTGATGCTCATCTACA+GGG + chr1.1:6052380-6052399 MS.gene33012:CDS 40.0%
TCTTGAGTGAGTATAGTCAG+AGG - chr1.1:6052147-6052166 None:intergenic 40.0%
TTAGTGTGATGCTCATCTAC+AGG + chr1.1:6052379-6052398 MS.gene33012:CDS 40.0%
TTATTGAGAGAATTGGCCAG+CGG + chr1.1:6051854-6051873 MS.gene33012:CDS 40.0%
TTTGGGAGAGAAGAGAAAGA+AGG - chr1.1:6050519-6050538 None:intergenic 40.0%
! AAGCTCATTGGTTGCACTAA+TGG + chr1.1:6051791-6051810 MS.gene33012:intron 40.0%
! ACAGGGAGCGTAAGTTTAAA+AGG + chr1.1:6052397-6052416 MS.gene33012:CDS 40.0%
! CTGATAACGATAGAGTCTTG+TGG + chr1.1:6050943-6050962 MS.gene33012:CDS 40.0%
! GGAATTATAGGGGTAGCTTT+TGG + chr1.1:6050897-6050916 MS.gene33012:CDS 40.0%
! GGACTCTCATATCTTGTTGT+TGG + chr1.1:6053155-6053174 MS.gene33012:intron 40.0%
! TTTGGAACACTTGTGCTTAG+CGG + chr1.1:6052341-6052360 MS.gene33012:CDS 40.0%
!! CGCCAAATTCTAGTTTGTCT+AGG + chr1.1:6052622-6052641 MS.gene33012:intron 40.0%
!! CTCATATCTTGTTGTTGGCT+TGG + chr1.1:6053160-6053179 MS.gene33012:intron 40.0%
!! TTGGTTTCTGGGTTTTTCAC+AGG + chr1.1:6050656-6050675 MS.gene33012:intron 40.0%
!!! GCAACTAGGGAGTGTTTTAA+AGG - chr1.1:6051621-6051640 None:intergenic 40.0%
AAGTAAAGGCTGCGAAAAGG+AGG - chr1.1:6051903-6051922 None:intergenic 45.0%
AGTAAAGGCTGCGAAAAGGA+GGG - chr1.1:6051902-6051921 None:intergenic 45.0%
CACAAGTAAAGGCTGCGAAA+AGG - chr1.1:6051906-6051925 None:intergenic 45.0%
CTCAAGCAGAGATATGGTCT+TGG + chr1.1:6050859-6050878 MS.gene33012:CDS 45.0%
GATAAACCCCTGTGCCAAAA+AGG - chr1.1:6052248-6052267 None:intergenic 45.0%
GTGATCACATTCACCGTGTT+GGG - chr1.1:6050686-6050705 None:intergenic 45.0%
GTTGCACTAATGGCAGATGA+TGG + chr1.1:6051801-6051820 MS.gene33012:intron 45.0%
TAAACCAAAGTCAGGCTGTC+TGG + chr1.1:6051249-6051268 MS.gene33012:intron 45.0%
TGATCACATTCACCGTGTTG+GGG - chr1.1:6050685-6050704 None:intergenic 45.0%
TGATGCGAGCAGTGAGTTTA+TGG + chr1.1:6051477-6051496 MS.gene33012:intron 45.0%
TGTGATCACATTCACCGTGT+TGG - chr1.1:6050687-6050706 None:intergenic 45.0%
!! AGTAGGAGAGCGTGGAATTT+TGG - chr1.1:6050537-6050556 None:intergenic 45.0%
!! GTAGGAGAGCGTGGAATTTT+GGG - chr1.1:6050536-6050555 None:intergenic 45.0%
!! TGTTGGCTTGGTGACAGATT+AGG + chr1.1:6053172-6053191 MS.gene33012:intron 45.0%
AAGCTCCATCTCACATGCTG+CGG + chr1.1:6052677-6052696 MS.gene33012:intron 50.0%
GAGAGTCCAAAGCTCTTCCA+AGG - chr1.1:6053143-6053162 None:intergenic 50.0%
GATTGCGCAAGTCAGCGAAT+TGG - chr1.1:6050565-6050584 None:intergenic 50.0%
GCAGCTGCTGCAATAGCTAA+AGG - chr1.1:6052113-6052132 None:intergenic 50.0%
TACAACACATCCCAATCCGC+TGG - chr1.1:6051873-6051892 None:intergenic 50.0%
TCTAGGCTTTCGAGACCATC+AGG + chr1.1:6052639-6052658 MS.gene33012:intron 50.0%
TTTCACAGGTGTCCCCAACA+CGG + chr1.1:6050670-6050689 MS.gene33012:intron 50.0%
! AGAGAATTGGCCAGCGGATT+GGG + chr1.1:6051860-6051879 MS.gene33012:CDS 50.0%
! AGTCTTGTGGGACACTTTGC+CGG + chr1.1:6050956-6050975 MS.gene33012:CDS 50.0%
! GCACTAATGGCAGATGATGG+TGG + chr1.1:6051804-6051823 MS.gene33012:intron 50.0%
! GGACAGATGTTGTGGTCTTG+TGG - chr1.1:6052553-6052572 None:intergenic 50.0%
!! AAGCCTGACCTTTTTGGCAC+AGG + chr1.1:6052237-6052256 MS.gene33012:intron 50.0%
!! AGCCTGACCTTTTTGGCACA+GGG + chr1.1:6052238-6052257 MS.gene33012:intron 50.0%
!! GCTGACTTGCGCAATCTTCT+TGG + chr1.1:6050569-6050588 MS.gene33012:CDS 50.0%
!! TCAGCGAATTGGTGGTGAGT+AGG - chr1.1:6050554-6050573 None:intergenic 50.0%
ACCCCTGTGCCAAAAAGGTC+AGG - chr1.1:6052243-6052262 None:intergenic 55.0%
CGTTATCAGCGGGCACTCTT+TGG + chr1.1:6052323-6052342 MS.gene33012:CDS 55.0%
GAGAGAATTGGCCAGCGGAT+TGG + chr1.1:6051859-6051878 MS.gene33012:CDS 55.0%
TCTGCCAGACAGCCTGACTT+TGG - chr1.1:6051256-6051275 None:intergenic 55.0%
TGCGCAAGTCAGCGAATTGG+TGG - chr1.1:6050562-6050581 None:intergenic 55.0%
TTCGACCGCAGCATGTGAGA+TGG - chr1.1:6052685-6052704 None:intergenic 55.0%
TTGTGGGACACTTTGCCGGT+TGG + chr1.1:6050960-6050979 MS.gene33012:intron 55.0%
!! GCCTGACCTTTTTGGCACAG+GGG + chr1.1:6052239-6052258 MS.gene33012:intron 55.0%
! TGGTGGTGAGTAGGAGAGCG+TGG - chr1.1:6050545-6050564 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 6050392 6053216 6050392 ID=MS.gene33012
chr1.1 mRNA 6050392 6053216 6050392 ID=MS.gene33012.t1;Parent=MS.gene33012
chr1.1 exon 6050392 6050590 6050392 ID=MS.gene33012.t1.exon1;Parent=MS.gene33012.t1
chr1.1 CDS 6050392 6050590 6050392 ID=cds.MS.gene33012.t1;Parent=MS.gene33012.t1
chr1.1 exon 6050678 6050777 6050678 ID=MS.gene33012.t1.exon2;Parent=MS.gene33012.t1
chr1.1 CDS 6050678 6050777 6050678 ID=cds.MS.gene33012.t1;Parent=MS.gene33012.t1
chr1.1 exon 6050857 6050977 6050857 ID=MS.gene33012.t1.exon3;Parent=MS.gene33012.t1
chr1.1 CDS 6050857 6050977 6050857 ID=cds.MS.gene33012.t1;Parent=MS.gene33012.t1
chr1.1 exon 6051817 6051938 6051817 ID=MS.gene33012.t1.exon4;Parent=MS.gene33012.t1
chr1.1 CDS 6051817 6051938 6051817 ID=cds.MS.gene33012.t1;Parent=MS.gene33012.t1
chr1.1 exon 6052064 6052185 6052064 ID=MS.gene33012.t1.exon5;Parent=MS.gene33012.t1
chr1.1 CDS 6052064 6052185 6052064 ID=cds.MS.gene33012.t1;Parent=MS.gene33012.t1
chr1.1 exon 6052259 6052418 6052259 ID=MS.gene33012.t1.exon6;Parent=MS.gene33012.t1
chr1.1 CDS 6052259 6052418 6052259 ID=cds.MS.gene33012.t1;Parent=MS.gene33012.t1
chr1.1 exon 6053189 6053216 6053189 ID=MS.gene33012.t1.exon7;Parent=MS.gene33012.t1
chr1.1 CDS 6053189 6053216 6053189 ID=cds.MS.gene33012.t1;Parent=MS.gene33012.t1
Gene Sequence

>MS.gene33012

ATGATGATTCAGAATTGGAGTTTGAATTGGATGGTAGCACATGCAAACAAACAACAACAACAATTGAGAAGATTGAAGTTAACAACTCATCGTGTTATTGTAGCTACATTTCTCTTCTCTATCTCCTTCTTTCTCTTCTCTCCCAAAATTCCACGCTCTCCTACTCACCACCAATTCGCTGACTTGCGCAATCTTCTTGGTAAAAAATTTCACTTTCTATTTAATTTGATGAAAAAGATTTCATTTTTCTTAATTTGGATGGTTTTGGTTTCTGGGTTTTTCACAGGTGTCCCCAACACGGTGAATGTGATCACAAATTTCCCATTTTTAGTTGTGGGTGTTCTTGGTTTAGTTCTTACTCTTGAAGGAGGATTTTTTACCGTAAGGTTAGTTACTTTTATCATCACTGTAATAGAATTTACTGTGATTAGATTCTAATATGAGGTGTGTTTTTGATTTGGTTAGTTCTCAAGCAGAGATATGGTCTTGGATACTATTCTATGCTGGAATTATAGGGGTAGCTTTTGGCTCTGTTTATTACCATTTGAAGCCTGATAACGATAGAGTCTTGTGGGACACTTTGCCGGTTGGTTAGCATTCCCATTACCTACCTCTCTATATGAATATATTTATATATGTTATTACTATTTGTATGATTCTTGAGTTGTTTTCCTTTTCCATGATTGTTTTGGCTAAAGAACTTAATTAAGCACTTACTCGATACACACTTGTCAAGTTATCATATAAGAGTTTATGTGTAAGCTATTTCAATAATGTAAGGCCTAGTTTGGATAAACAACTTAATTAAGCGCTTATCATATAAGTGCTTATGTATAAGTTATTTCTGTAACAAAAGATAAACCAAAGTCAGGCTGTCTGGCAGAGCTTACGAAAATAAGCTAAAAATAGCTTATGGACATGTCATAAGCTGTTTTATAAGCTCTCTCAAAACTCTCACAAATACGTAGATAAGCTCAAATAAGTCAATCCGAGAAGACACTACGCATGTATAAACTATTTCTATATTTGAATAGGGGATGCGGTAAAACTCCACATAAGCTCTCTTCTATAATTCTATTAAGTTATGATGCGAGCAGTGAGTTTATGGAAATAAGCTCAACACAACTTATAAACTTGTCATAAGCTCTTTTTAAAATTAATCTCTTCAAAAATTATTCCAAACTCACACAAGTGTTATTGATGTCATTAGATAAAGCCAAATAAGCCCTTTAAAACACTCCCTAGTTGCTCCATTTGTTTAAATTATGACAGAAGTGAGCACTTATAAAGAGAAAATGTTAAGGTGGAATGCATTTTATCTGTTAAAAACACTTCTTTTGCTAGCTGAAAATCTAGACTTACCTCTTAGTACTTAGTTCGTTTTTTCCCCTTTATTAAGAAGCTCATTGGTTGCACTAATGGCAGATGATGGTGGCATATTCCTCACTTTTCTCTAGTTTGGTTATTGAGAGAATTGGCCAGCGGATTGGGATGTGTTGTATGTGTGCCCTCCTTTTCGCAGCCTTTACTTGTGTTGTTTATGAACGGTTAGTTCTTATCTTCCTCTAATAATAGTCTCAAGCTTATTCTCTCTTTTAAATGTTTTATACTTTGAGGTGCTGAACTTGCCACTAAGTTTATGCTAAAATTATGATCTGCTAGTTGGTTGCAGAACTTATGATGATATTCGGCTCTGCTTGATGTTCCAGTTCATTCTTCCTTTAGCTATTGCAGCAGCTGCATTTGTATATCCCTCTGACTATACTCACTCAAGATATTGGTTTTCATCAATAGGTATTATGCCTCAGACTTTTGCAACATTTATTCATAAATTTCAAATATTTGAAGCCTGACCTTTTTGGCACAGGGGTTTATCTGCTAGCAAAATTTGAAGCTGTTTCTGACAAGAAACTCTACCGTGCAAATAACTACGTTATCAGCGGGCACTCTTTGGAACACTTGTGCTTAGCGGTTATCCCGTTTTTACTTAGTGTGATGCTCATCTACAGGGAGCGTAAGTTTAAAAGGTATTTGATGATGAACCTATTCGATGGAATTTTTGGTTCCTTGTGAAATTTGGTTTCACAAATTGTTCTGGTTATTCTGTTTCTACAAATTTTTGAGTGAATCCTCAAGTCAGTTTTCCAAATTCAAACATCCACAAGACCACAACATCTGTCCTTCAAAAATTTGAAATGTCATCCCACTAGTTTTTTTCTTCAGAAGTAGTCGCCAAATTCTAGTTTGTCTAGGCTTTCGAGACCATCAGGAATCGATTTTGATCAAAGCTCCATCTCACATGCTGCGGTCGAAATCGATTTTTACCTCACCATGAATTGGTTTTTGAATAGGCTATTAGCACGTGTTTGTTTTGGTGATTGACAAAAAGTGAACGTGCATTGCTTTGTTAGCTACAATTTGTAGCTTGTGCATTTCCCCTGATTGACTTATTTTAGTTTATATACTGATATAAACATTTTTGAGACTGTTTGTGATAGCTAATGAAAACAGCTTATCATAATCTCTGTGATAAGCTGTTTTAAGCTTATTTTCATAAGCCCTCCAAGATATCTTATGAAAACAATTTATAGCTTATATGAAAACAACTTGACTTTCTTTTATCTTTTGTTATATAAATAACTTATATATAAACAATTATATGATAAATGCTTATGCTATAAGCACTTAATTGTGGAGAACAACGTTGAAGGTTTACTATTCTCTTTTTCCTTTTTGTAATTAATGTTTCCTTGGAAGAGCTTTGGACTCTCATATCTTGTTGTTGGCTTGGTGACAGATTAGGAGATGTTAAAGATCAGCTTTGA
Protein sequence

>MS.gene33012.t1

MMIQNWSLNWMVAHANKQQQQLRRLKLTTHRVIVATFLFSISFFLFSPKIPRSPTHHQFADLRNLLGVPNTVNVITNFPFLVVGVLGLVLTLEGGFFTVSSQAEIWSWILFYAGIIGVAFGSVYYHLKPDNDRVLWDTLPMMVAYSSLFSSLVIERIGQRIGMCCMCALLFAAFTCVVYERTYDDIRLCLMFQFILPLAIAAAAFVYPSDYTHSRYWFSSIGVYLLAKFEAVSDKKLYRANNYVISGHSLEHLCLAVIPFLLSVMLIYRERKFKRLGDVKDQL